CN106495829A - Nutritional solution for rice rich in selenium Rice Cropping - Google Patents
Nutritional solution for rice rich in selenium Rice Cropping Download PDFInfo
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- CN106495829A CN106495829A CN201610869554.4A CN201610869554A CN106495829A CN 106495829 A CN106495829 A CN 106495829A CN 201610869554 A CN201610869554 A CN 201610869554A CN 106495829 A CN106495829 A CN 106495829A
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- rice
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses for the nutritional solution of rice rich in selenium Rice Cropping, being prepared by the following preparation method:1) raw material prepares:Prepare 100 parts of pure water, 1.5 2.5 parts of L Selenomethionines, 0.5 1.0 parts of hydroxyethyl cellulose;2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa, it is heated to 150 DEG C, 2min is kept, then quick release pressure obtains serosity in 1 3s, be 5 10% to access the ripe bacillus subtilises liquid spawns of culture at 25 DEG C by percent by volume, it is put in fermentation tank, filtrated air is passed through in fermentation tank, fermentation is stirred, after fermentation ends, temperature is risen to 70 DEG C rapidly and keeps 0.5 1.0h inactivations, obtained.
Description
Technical field
The present invention relates to Rice Cropping technical field, the more particularly, to nutritional solution of rice rich in selenium Rice Cropping.
Background technology
Oryza sativa L., originates in China, and Oryza sativa L. knot is Oryza glutinosa, claims rice or rice, be world's staple food crop after shelling
One of.Before 7000 years, Yangtze River in China basin is with regard to rice cultivation.Rice in China sowing face accounts for the 1/4 of national cereal crops, and yield
More than half is then accounted for.According to different methods, Oryza sativa L. can be divided into long-grained nonglutinous rice and japonica rice, early rice and middle late rice, Oryza sativa L. var. glutinosa Matsum. and non-Oryza sativa L. var. glutinosa Matsum..
Oryza sativa L. knot is Oryza glutinosa, and Oryza glutinosa (grain) claims rice, scented rice, rice after shelling.Nearly half population in the world, all with rice be
Food.The existing 14000-18000 of cultivation history.For important cereal crops, can starch processed, wine brewing, vinegar processed, Testa oryzae can refine sugar, squeeze
Oil, extraction furfural, for industrial and pharmaceutical;Rice straw is good feedstuff and paper making raw material and braided material, and Fructus Setariae Germinatuss and rice root are available for
Medicinal.The eating method of rice is varied, has rice, congee, rice cake, rice cake, rice wine etc..Oryza sativa L. may be used also in addition to edible
Make the raw material of industry to make wine, refine sugar, rice husk, rice straw can be used as feedstuffs.China Oryza sativa L. main producing region is mainly the Northeast, length
River basin, Pearl River Delta.Belong to direct economy crop.Still the staple food of 1/3rd mankind in the world.
Selenium is trace element needed by human.Selenium participates in multiple selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang
Sweet peptide peroxidase, is catalyzed hydroperoxides in vivo or lipid peroxide is changed into water or various alcohols, eliminates
Free radical protects biomembrane from oxidative damage to biomembranous attack;Selenium participates in constituting the de- iodine enzyme of iodate thyroid amino acid.?
Selenium and vitamin E collaboration, can protect cell membrane, prevent the oxidation of unsaturated fatty acid in vivo.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, promote the propagation of lymphocyte and the synthesis of antibody and immunoglobulin.
Selenium has the effect for significantly suppressing and protecting to kinds cancers such as colon cancer, skin carcinoma, hepatocarcinoma, breast carcinoma, and which is in body
Mesostate methyl enol have stronger active anticancer.Selenium and the battalion such as vitamin E, garlicin, linoleic acid, germanium, zinc
Support element and there is collaboration antioxidative effect, increase antioxidant activity.Meanwhile, selenium has the work for mitigating and alleviating heavy metal toxicity
With.
Scientific circles' research finds that the height of blood selenium level is closely bound up with the generation of cancer.Substantial amounts of survey data explanation, one
Individual regional food and the height of Selenium in Soils content have direct relation with the sickness rate of cancer, for example:This regional food and soil
Se content in earth is high, and the M & M of cancer is just low, conversely, this regional cancer morbidity and mortality rate are just
The generation close relation of height, true explanation selenium and cancer.There is prophylaxis of cancer to act on while scientific circles also identify selenium, be
" king of anti-cancer " of body trace element.As inorganic selenium salt toxicity is larger, forbid in food and feedstuff within 1993 in Japan
Middle interpolation, supports the organic form selenium such as the Selenonic protein higher using safety, aminoacid, or Se-rich farm products.
At present to Selenium rich rice, the research of rice rich in selenium, such as Chinese patent CN1608435A, a kind of Selenium rich rice
Cultural method, discloses a kind of selenium-rich agent cultivation selenium-rich water being made up of foliage-spray inorganic compound containing selenium and phosphate
The method of rice;CN101228834A, a kind of production method of nutritious rice rich in Se, disclose a kind of in paddy growth not
Same time, the mixed liquor being mixed by RE microbial fertilizer, sodium selenite and boric acid solution is sprayed, to produce selenium-rich nutritional rice
Method;CN1844062A is a kind of to spray selenium-enriched fertilizer and preparation method thereof in leaf, disclose a kind of containing selenium and with not on year-on-year basis
Foliage fertilizer formulation of composition such as trace element and preparation method thereof in the multiple beneficial such as the nitrogen of example, phosphorus, potassium and zinc, magnesium, copper.
Above-mentioned prior art is all that the selenium using inorganic selenium (selenate or selenite) as fertilizer is originated, and inorganic selenium are remained
Amount is big, and inorganic selenium have obvious toxic action to body, and safety is relatively low;Using the method for foliage-spray inorganic selenium, which is excellent
Point is that Crop is fast, but its shortcoming is it is also obvious that foliage-spray requires that selenium fertilizer comparision contents are high first, such as
CN1382374A, the content of the salt of selenic acid or Monohydrated selenium dioxide in plant rich in selenium nutrition regulating agent is 4-25%, the selenium fertilizer of high concentration
Increased with fertile cost.
Content of the invention
It is an object of the invention to provide for the nutritional solution of rice rich in selenium Rice Cropping, by spraying on rice tillering latter stage blade face
Apply the nutritional solution, reach realize rice high yield increase income, the effect of selenium element stable content.
Nutritional solution for rice rich in selenium Rice Cropping of the present invention, is prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, L- Selenomethionine 1.5-2.5 parts, hydroxyethyl cellulose 0.5-1.0 parts;
2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keep 2min,
Then quick release pressure obtains serosity in the 1-3s, is that 5-10% accesses the ripe hay of culture at 25 DEG C by percent by volume
Bacillus cereuss liquid strain, is put in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, after fermentation ends, fast
Temperature is risen to 70 DEG C and keeps 0.5-1.0h inactivations by speed, is obtained.
Step 1 of the present invention) described in raw material, preferably 100 parts of pure water, 2.0 parts of L- Selenomethionines, hydroxyethyl cellulose
0.8 part, described L- Selenomethionines, i.e. L- selenomethionines are also called Selenomethionine.
Step 2) described in the preferred 250r/min of speed of agitator, the fermentation, preferable temperature are kept for 35-37 DEG C, during fermentation
Between be 3-4h.
The cultural method of the bacillus subtilises liquid spawn is:Using triangular flask strain cultivation, below attach most importance to
Amount percent, by albumen slurry 30.0-35.0% for processing, KH2PO41.0%, MgSO42.0%, NaCl0.5% are put into
In triangular flask, stirring, pH being adjusted to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, access through the withered of slant culture
Careless Bacillus, 28 DEG C of shaking table cultures 60-100h are inoculated with for fermentation tank.
Nutritional solution of the present invention is used for rice rich in selenium Rice Cropping, sprays nutritional solution 1-2 time in rice tillering latter stage, preferably general
Nutritional solution is sprayed after diluting 3-5 times, so that blade face uniform fold to be defined.
Compared to existing technology, it is an advantage of the current invention that:
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium
Sour potassium and potassium selenate etc., obtain from the side-product of metal mineral reserves, and inorganic selenium have larger toxicity, and are difficult to be absorbed, as
Nutritional solution, assimilation effect are bad.The Selenomethionine that the present invention is used, is a kind of organic selenium source, compared with inorganic selenium, seleno egg ammonia
Acid has safe and efficient advantage, and it can substitute some methionine and be attached to the reversible selenium storage of formation one on body protein
Storehouse, and there is no toxic hazard.
2nd, Selenomethionine is that selenium is present in a kind of seleno amino in plant and feed grain with organic form in nature
The plant growings such as acid, frumentum and forage crop are not necessarily required to Selenomethionine, but with methionine synthesis they can utilize ring
The selenite and selenate synthesis Selenomethionine being particularly in border in soil.More using selenium in soil, in plant
Selenomethionine content is higher.At present, Selenomethionine is mainly derived from natural selenium yeast, is that organic selenium is primarily present in selenium yeast
Form, but Selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether final product
The taste and flavor of product.Bacillus subtillis, are one kind of Bacilluss, and bacillus subtilises thalline itself synthesizes alphalise starch
The enzymes such as enzyme, protease, Digestive Enzyme, cellulase, equivalent to compound enzyme, the present invention is by Selenomethionine mixing Ko subtilis bar
Bacterium after fermentation, is fermented using the zymasises that Selenomethionine product is carried, on the one hand enters Oryza sativa L. beneficial to selenium element, on the other hand
With methionine synthesis they can utilize in environment selenite and selenate synthesis Selenomethionine in particularly soil, also
Can be effectively improved, dissolve yeast flavor.
3rd, steam explosion be using high-temperature high-pressure steam process biomass material, and realized by moment pressure leak process right
The surface texture change of raw material, Component seperation and hemicellulose degradation, are generally used for corn straw, straw and Wooden Biomass etc.
The pretreatment of raw material.The present invention is by " being put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keeping 2min, then in 1-
Quick release pressure in 3s ", on the one hand can change to sterilize by the moment of pressure, on the other hand can effectively refine, divide
Scattered Selenomethionine, advantageously in the absorption of Oryza sativa L..
4th, the present invention with the addition of hydroxyethyl cellulose in nutritional solution, with good thickening, suspensions, dispersion, emulsifying, glue
The characteristics such as conjunction, film forming, protection moisture and offer protecting colloid, can cause Selenomethionine fully to stick to used in nutritional solution
On blade face, branch, beneficial to absorption of the blade face to nutritional labeling, the rice obtained using the technology of the present invention, according to GB/T 22499-
2008《The standard test of selenium-rich Oryza glutinosa》In rice, the content of selenium is 0.27-0.38mg/kg.
Specific embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1:
For the nutritional solution of rice rich in selenium Rice Cropping, it is prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, 1.5 parts of L- Selenomethionines, 1.0 parts of hydroxyethyl cellulose;
2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keep 2min,
Then quick release pressure obtains serosity in the 1-3s, is the ripe hay bud of 5% access culture at 25 DEG C by percent by volume
Spore bacillus liquid spawn, is put in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator 250r/
Min, fermentation time are 3h, and temperature is kept for 35-37 DEG C, after fermentation ends, temperature is risen to 70 DEG C rapidly and keeps 0.5h inactivations, obtained
Arrive.
Embodiment 2:
For the nutritional solution of rice rich in selenium Rice Cropping, it is prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, 2.5 parts of L- Selenomethionines, 0.5 part of hydroxyethyl cellulose;
2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keep 2min,
Then quick release pressure obtains serosity in the 1-3s, is the ripe hay bud of 10% access culture at 25 DEG C by percent by volume
Spore bacillus liquid spawn, is put in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator 250r/
Min, fermentation time are 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, temperature is risen to 70 DEG C rapidly and keeps 1.0h inactivations, obtained
Arrive.
Embodiment 3:
For the nutritional solution of rice rich in selenium Rice Cropping, it is prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, 2.0 parts of L- Selenomethionines, 0.8 part of hydroxyethyl cellulose;
2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keep 2min,
Then quick release pressure obtains serosity in the 1-3s, is the ripe hay bud of 8% access culture at 25 DEG C by percent by volume
Spore bacillus liquid spawn, is put in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator 250r/
Min, fermentation time are 3h, and temperature is kept for 35-37 DEG C, after fermentation ends, temperature is risen to 70 DEG C rapidly and keeps 0.5h inactivations, obtained
Arrive.
Comparative example:
For the nutritional solution of rice rich in selenium Rice Cropping, it is prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, 2.0 parts of L- Selenomethionines, 0.8 part of hydroxyethyl cellulose;
2) by step 1) raw material mixing after obtain.
Experimental example:
The three batch rice obtained using the embodiment of the present invention 3, the selenium element content of rice is 0.27mg/ respectively after testing
kg、0.30mg/kg、0.38mg/kg;
The three batch rice obtained using comparative example, after testing the selenium element content of rice be respectively 0.08mg/kg,
0.07mg/kg、0.06mg/kg.
Claims (4)
1. the nutritional solution of rice rich in selenium Rice Cropping is used for, it is characterised in that be prepared by the following preparation method:
1) raw material prepares:Prepare 100 parts of pure water, L- Selenomethionine 1.5-2.5 parts, hydroxyethyl cellulose 0.5-1.0 parts;
2) by step 1) raw material mixing after, be put into steam explosion tank, pressure 4MPa is heated to 150 DEG C, keep 2min, then
Quick release pressure obtains serosity in the 1-3s, is that 5-10% accesses the ripe hay spore of culture at 25 DEG C by percent by volume
Bacillus liquid spawn, is put in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, after fermentation ends, rapidly will
Temperature rises to 70 DEG C and keeps 0.5-1.0h inactivations, obtains.
2. the nutritional solution for rice rich in selenium Rice Cropping according to claim 1, it is characterised in that:Step 1) described in original
Material, is 100 parts of pure water, 2.0 parts of L- Selenomethionines, 0.8 part of hydroxyethyl cellulose.
3. the nutritional solution for rice rich in selenium Rice Cropping according to claim 1, it is characterised in that:Step 2) described in stir
Mix rotating speed 250r/min, the fermentation, temperature are kept for 35-37 DEG C, and fermentation time is 3-4h.
4. the nutritional solution for rice rich in selenium Rice Cropping according to claim 1, it is characterised in that:Step 2) hay
The cultural method of bacillus cereuss liquid strain is:Using triangular flask strain cultivation, it is percetage by weight below, will processes
Albumen slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl0.5% are put in triangular flask, are stirred,
PH is adjusted to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, Bacillus subtilis strain of the access through slant culture, 28 DEG C
Shaking table culture 60-100h, is inoculated with for fermentation tank.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107382519A (en) * | 2017-08-28 | 2017-11-24 | 广西钟山县富强雅瑶华香农业有限公司 | A kind of selenium-rich rice plants nutrient solution |
CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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CN102415252A (en) * | 2011-08-23 | 2012-04-18 | 南京远望富硒农产品有限责任公司 | Method for preparing selenium-rich rice |
CN102503632A (en) * | 2011-10-25 | 2012-06-20 | 惠州市海纳粮油食品有限公司 | Selenium-rich organic/inorganic fertilizer for producing selenium-rick rice as well as application method thereof |
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CN105272761A (en) * | 2015-11-16 | 2016-01-27 | 南宁穗康农业科技有限公司 | Selenium-enriched biological organic fertilizer and production method thereof |
CN105950503A (en) * | 2016-05-19 | 2016-09-21 | 湖北民族学院 | Bacillus subtilis SE201412 and organic-selenium-rich bactericide |
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CN102391047A (en) * | 2011-08-08 | 2012-03-28 | 江苏浩波农业科技开发有限公司 | Sustained-release soil selenium fertilizer used for degrading residual pesticide and reducing heavy metal content, and preparation method thereof |
CN102415252A (en) * | 2011-08-23 | 2012-04-18 | 南京远望富硒农产品有限责任公司 | Method for preparing selenium-rich rice |
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Cited By (2)
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CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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Application publication date: 20170315 |