CN106416973A - Seed-germination-visualization culturing medium and preparing method and application thereof - Google Patents
Seed-germination-visualization culturing medium and preparing method and application thereof Download PDFInfo
- Publication number
- CN106416973A CN106416973A CN201610823226.0A CN201610823226A CN106416973A CN 106416973 A CN106416973 A CN 106416973A CN 201610823226 A CN201610823226 A CN 201610823226A CN 106416973 A CN106416973 A CN 106416973A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culturing medium
- germination
- plant
- visualization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention relates to a seed-germination-visualization culturing medium and a preparing method and application thereof. Every 1 L of the culturing medium is prepared from 0.55 g-0.65 g of KNO3, 0.9 g-1.0 g of Ca(NO3)2.4H2O, 0.2 g-0.3 g of NH4H2PO4, 0.2 g-0.3 g of MgSO4.7H2O, 2.00 g-4.00 g of a coagulator and the balance water. The culturing medium is a toxicity-injury-free transparent solid culturing medium, can replace soil to allow seeds to germinate and grow on the culturing medium, and can provide the moisture and the nutrients required by growth of plants. The culturing medium is highly transparent, and the growth of plant roots can be clearly observed. When the culturing medium is adopted, a user only needs to place soaked seeds on the surface of the culturing medium, watering is avoided, the seeds can germinate and grow after 2 days to 3 days, and the user can visibly and clearly observe the whole growing process of plant root systems and buds.
Description
Technical field
The present invention relates to a kind of transparent solid culture medium with agarose as main component, and its in plant growing and culture
The application of aspect.
Background technology
Cultivation of plants mode mainly has earth culture, colored mud of water planting, ornamental crystal mud cultivation and flower arrangement use etc. at present.
Normal soil cultivation is as modal traditional cultivation mode at present, although easy and simple to handle during planting plants, cultivation
Effect is preferable, soil sources are extensively easy to get, but cannot clearly represent the growing way of root in growing process.For ensureing plant length
Gesture, earth culture plant need to periodically be watered, and this will arrange plant to the need crowd of often travelling on business trips and make troubles.
Compare soil cultivation, although the transparency of plant root is substantially increased it can be observed that planting using water planting mode
The root of thing, but water planting environment can not provide adhesive force needed for growth for root system of plant, so water planting mode cannot represent plant
The growing way of thing root in its natural state.And hydroponic plant growing way is substantially not so good as soil cultivation, or even some plants cannot at all
Cultivated using water planting mode.Another defect of water planting mode is that plant culture is liquid, and preservation is had with transport and is necessarily stranded
Difficult.Secondly hydroponic plant need to be refreshed the water periodically, and brings certain inconvenience to plant culture.
Although crystal mud is glittering and translucent, color is bright and glittering, and soil, peat, loose phosphorus, volcanic rock, Vermiculitum etc. can be replaced
Substrate is gone to plant and is grown flowers and nursed the grass, but itself has toxicity, has certain risk in particular for child when therefore using.And crystal mud
Formula and processing mode are relative complex, relatively costly during processing.
Content of the invention
In order to solve problem above, the present invention provides a kind of low cost, nontoxic, and transparency is high, is increasing plants ' aestheticses
While also can represent the form that root system of plant grows in underground germination visualization culture medium.
The technical solution used in the present invention is：A kind of germination visualizes culture medium, and described culture medium consists of：Often
1L culture medium contains：
KNO30.55-0.65g、Ca(NO3)2·4H2O 0.9-1.0g、NH4H2PO40.2-0.3g、
MgSO4·7H2O 0.2-0.3g, coagulator 2.00-4.00g, water surplus.
Preferably, above-mentioned germination visualization culture medium, described culture medium consists of：Every 1L culture medium contains,
KNO30.61g、Ca(NO3)2·4H2O 0.94g、NH4H2PO40.23g、
MgSO4·7H2O 0.25g, coagulator 3.00g, water surplus.
Preferably, above-mentioned germination visualization culture medium, described coagulator is agarose.
A kind of germination visualizes the preparation method of culture medium, and method is as follows：Weigh 0.55-0.65g KNO3、0.9-
1.0g Ca(NO3)2·4H2O、0.2-0.3g NH4H2PO4、0.2-0.3g MgSO4·7H2O, 2.00-4.00g coagulator, adds
It is sufficiently stirred in distilled water configuring 1L solution, boil rear room temperature and place to solidification..
Above-mentioned germination visualization application in plant growing and culture for the culture medium.Method is as follows：By culture medium
It is placed in container, after then plant seed being soaked one day in clear water, be directly put in media surface.
The invention has the beneficial effects as follows：
1. the culture medium of the present invention, is a kind of nontoxic, transparent solid plant culture of alternative soil.This culture medium
Alternative soil provides growth adhesive force and highly transparent for root system of plant, and user can be clearly observable root system of plant in soil
Natural growth conditions in earth, increase and also can represent the form in underground for the root system of plant while plants ' aestheticses.
2. the culture medium of the present invention, alternative water planting planting type in terms of transparency and sight, and culture medium is solid
Body state, comparing fluid medium, to carry transport more convenient.
3. the culture medium of the present invention, containing nutrient substance necessary to plant growing and moisture, need not periodically pour during plantation
The water and preferable growing way of plant can be ensured, it is possible to resolve often the crowd of going on business arranges the problem of flowers and plants inconvenience.
4. the culture medium of the present invention, formula is nontoxic, safety and environmental protection, and child also can be safe to use.In addition, culture basigamy
Fang Chengfen is simple, and operating process is simple and easy, compares existing crystal mud production technology, can substantially reduce production cost.
5. the present invention, method is easy to learn, and the plant seed of required cultivation only need to be soaked one day by user with clear water, puts
Plant in media surface, culture medium be placed in sunny place, next week of room temperature left and right plant will complete from seed
Sprout the process growing into seedling.Growing process need not water and apply nutritional solution.Consumer can be at one week
In observe plant roots and the continuous growth course of bud, the root system of plant particularly not observed at ordinary times life in its natural state
Long status.
Specific embodiment
Embodiment 1
(1) formula
Germination visualizes culture medium：Every 1L culture medium contains：0.61g KNO3、0.94g Ca(NO3)2·4H2O、
0.23g NH4H2PO4、0.25g MgSO4·7H2O, 3.00g agarose, water surplus.
(2) preparation method
Weigh 0.61g KNO3、0.94g Ca(NO3)2·4H2O、0.23g NH4H2PO4、0.25g MgSO4·7H2O、
3.00g agarose, adds and is sufficiently stirred in distilled water configuring 1L solution, boil rear room temperature and place to solidification.
(3) apply
Culture medium is placed in container, by plants such as the pulse families such as garden pea, Semen Tritici aestivi, Chinese cabbage, Radix Raphani, grass family, Cruciferae
Seed is directly put in media surface respectively after soaking one day in clear water and is cultivated.
(4) effect：
Place a plant into basal culture medium surface, next week of room temperature left and right plant will complete to sprout from seed to grow into children
The process of Seedling.Growing process need not water and apply nutritional solution, plant can be always maintained at preferable growth conditions,
And culture medium can keep highly transparent for a long time, consumer is sustainable to observe plant roots and the continuous growth course of bud, particularly
The root system of plant not observed at ordinary times growth conditions in its natural state.
Embodiment 2
The choice experiment of coagulator, the same embodiment of method, simply change the species of coagulator, result such as table 1.
Table 1
From table 1,5% sodium carboxymethyl cellulose transparency is best, but more sticky, is unsuitable for seed growth, and
Because solution temperature not can exceed that 80 DEG C, course of dissolution is complicated；Remaining coagulator transparency is all bad.Compare other compositions culture
Base, the transparency of agarose media preferably and compares suitable seed growth and transport.
Test result indicate that the agarose transparency of 3g/L is preferably, and it is suitable to plant growing.And agarose raw material is cheap, raw
Produce flow process simple, we determine using agarose as culture medium raw materials for production.
Claims (6)
1. a kind of germination visualization culture medium it is characterised in that：Described culture medium consists of：Every 1L culture medium contains,
2. germination according to claim 1 visualization culture medium it is characterised in that：Described culture medium consists of：
Every 1L culture medium contains,
3. a kind of germination visualization culture medium according to claim 1 and 2 it is characterised in that：Described coagulator
For agarose.
4. a kind of germination visualize culture medium preparation method it is characterised in that：Method is as follows：Weigh 0.55-
0.65gKNO3、0.9-1.0g Ca(NO3)2·4H2O、0.2-0.3g NH4H2PO4、0.2-0.3g MgSO4·7H2O、2.00-
4.00g coagulator, adds and is sufficiently stirred in distilled water configuring 1L solution, boil rear room temperature and place to solidification.
5. the germination visualization application in plant growing and culture for the culture medium described in claim 1 or 2.
6. application according to claim 5 is it is characterised in that method is as follows：Culture medium is placed in container, by plant speciess
Son is directly put in media surface after soaking one day in clear water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610823226.0A CN106416973A (en) | 2016-09-13 | 2016-09-13 | Seed-germination-visualization culturing medium and preparing method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610823226.0A CN106416973A (en) | 2016-09-13 | 2016-09-13 | Seed-germination-visualization culturing medium and preparing method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106416973A true CN106416973A (en) | 2017-02-22 |
Family
ID=58168152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610823226.0A Pending CN106416973A (en) | 2016-09-13 | 2016-09-13 | Seed-germination-visualization culturing medium and preparing method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106416973A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107396823A (en) * | 2017-08-28 | 2017-11-28 | 上海大学 | The method that quickly breeding transplants submerged plant |
| CN108409433A (en) * | 2018-04-25 | 2018-08-17 | 江苏医药职业学院 | A kind of new soil medium and its preparation method and application |
| CN115836641A (en) * | 2022-12-27 | 2023-03-24 | 广东省农业科学院农业质量标准与监测技术研究所 | Water culture seedling raising method for cruciferae hyper-enriched plants |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| CN2740436Y (en) * | 2004-01-15 | 2005-11-16 | 厦门博欣生物技术有限公司 | Ornamental plant products |
| US20060088935A1 (en) * | 2003-12-04 | 2006-04-27 | Shihe Fan | Method of ex vitro sowing, germination, growth and conversion of plant somatic embryos or germinants, and nutrient medium used therefor |
| CN101663968A (en) * | 2009-09-18 | 2010-03-10 | 华南农业大学 | Plant cultivating method for dynamically observing and measuring roots in situ |
| CN104302165A (en) * | 2011-12-21 | 2015-01-21 | 纳幕尔杜邦公司 | Plant artificial seeds having multilayers and methods for the production thereof |
-
2016
- 2016-09-13 CN CN201610823226.0A patent/CN106416973A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| US20060088935A1 (en) * | 2003-12-04 | 2006-04-27 | Shihe Fan | Method of ex vitro sowing, germination, growth and conversion of plant somatic embryos or germinants, and nutrient medium used therefor |
| CN2740436Y (en) * | 2004-01-15 | 2005-11-16 | 厦门博欣生物技术有限公司 | Ornamental plant products |
| CN101663968A (en) * | 2009-09-18 | 2010-03-10 | 华南农业大学 | Plant cultivating method for dynamically observing and measuring roots in situ |
| CN104302165A (en) * | 2011-12-21 | 2015-01-21 | 纳幕尔杜邦公司 | Plant artificial seeds having multilayers and methods for the production thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张传海 邓辉: "《植物生物技术》", 30 September 2015, 合肥工业大学出版社 * |
| 许加超: "《海藻化学与工艺学》", 30 September 2014, 中国海洋大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107396823A (en) * | 2017-08-28 | 2017-11-28 | 上海大学 | The method that quickly breeding transplants submerged plant |
| CN108409433A (en) * | 2018-04-25 | 2018-08-17 | 江苏医药职业学院 | A kind of new soil medium and its preparation method and application |
| CN115836641A (en) * | 2022-12-27 | 2023-03-24 | 广东省农业科学院农业质量标准与监测技术研究所 | Water culture seedling raising method for cruciferae hyper-enriched plants |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101960954B (en) | Liquorice planting method | |
| CN104429858B (en) | A kind of cultivation matrix of quickly breeding Banana Seedlings and preparation method thereof | |
| CN104322285B (en) | The seedling-cultivating method of a kind of brake | |
| CN103947531B (en) | A kind of Taxus media water planting making method for its potted landscape | |
| CN105613003B (en) | It is a kind of improve cane seedlings select index efficiency method for culturing seedlings and application | |
| CN105123183A (en) | Method for planting Suaeda salsa on saline-alkali land | |
| CN106416973A (en) | Seed-germination-visualization culturing medium and preparing method and application thereof | |
| CN103694039B (en) | The light cultivation matrix of Hubei Province, a kind of river Camellia fraternal breeding and container seedling culture method thereof | |
| CN105494065A (en) | Method for carrying out cutting seedling by Rosa multiflora Thunb seedling clods | |
| CN104365359A (en) | Tea tree planting method | |
| CN104221696A (en) | Jonquil large-scale atomizing cuttage seedling breeding method | |
| Oh et al. | Growth characteristics by plug tray cell size, soil type, and fertilizer concentration for plug seedling production of Veronica pusanensis YN Lee | |
| CN106258340A (en) | A kind of method for culturing seedlings of Paeonia suffruticosa | |
| CN105557430A (en) | Cutting seedling method for seedling block of rosa chinensis | |
| CN103229691B (en) | Constructing method for wide-narrow row J.sigillata Dode hard-twig cion cutting orchard | |
| CN102090310B (en) | Soilless culture substrate for violet | |
| CN105532398B (en) | A kind of hosta tissue culture seedling Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method | |
| CN107926530B (en) | Tomato seedling culture method | |
| CN102823504A (en) | Eucalypt tissue culture medium | |
| CN109566224A (en) | A kind of container seedling culture method and implantation methods of Ammopiptanthus mongolicus | |
| CN105638188B (en) | A kind of method for culturing seedlings of new pteris fern | |
| CN104737747A (en) | Propagation and culture method for red maple seedlings | |
| CN107197764A (en) | A kind of greenhouse cultivation method of kaffir lily | |
| CN103141368A (en) | Transparent device and method for directly observing relation between plant root growth and microorganism | |
| CN109757337B (en) | Method for culturing perilla substrate by using plant factory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |