CN106396910A - Pleurotus ostreatus culture medium and preparation method thereof - Google Patents
Pleurotus ostreatus culture medium and preparation method thereof Download PDFInfo
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- CN106396910A CN106396910A CN201610772701.6A CN201610772701A CN106396910A CN 106396910 A CN106396910 A CN 106396910A CN 201610772701 A CN201610772701 A CN 201610772701A CN 106396910 A CN106396910 A CN 106396910A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a pleurotus ostreatus culture medium comprising the following raw materials in parts by weight: 25-45 parts of eucalyptus bark, 25-40 parts of mulberry branches, 5-12 parts of wheat bran, 4-10 parts of peanut bran, 7-15 parts of corncob, 1.7-3 parts of a lime powder, 0.6-1.3 parts of gypsum, 0.2-0.5 part of calcium superphosphate, and 1-2 parts of sucrose. The main raw materials of the eucalyptus bark and the mulberry branches are rich in resources and low in price; nutrients contained in the prepared culture medium meet the growing demand of pleurotus ostreatus; the cultivated pleurotus ostreatus is pure white in color and luster, strong in growth vigor, large in mushroom size, thick in flesh, and tough in flesh layers, and a guarantee is provided for large-scale industrial production of the pleurotus ostreatus; the pleurotus ostreatus culture medium is conducive to vigorous development of mushroom industries, also makes full use of eucalyptus and mulberry resources, increases the processing added value, and protects the ecological environment, so use of the eucalyptus bark for culturing the pleurotus ostreatus has broad development prospects.
Description
Technical field
The present invention relates to culture medium of edible fungus technical field, particularly to a kind of oyster mushroom culture medium and preparation method thereof.
Background technology
Pleurotus ostreatus (scientific name:Pleurotus ostreatus;Also known as pick up the ears, oyster cap fungus, oyster mushroom, black Paeonia suffruticosa mushroom) be load
Agaricaless Pleurotaceae one species under bacterium door, is kind of a fairly common Lycoperdon polymorphum Vitt edible mushroom.Pleurotus ostreatus contain abundant nutrient substance, every hectogram
Dry product 20-23 containing protein gram, and aminoacid ingredient A wide selection of colours and designs, content of mineral substances very abundant, the traditional Chinese medical science thinks Pleurotus ostreatus
Temperature, sweet in the mouth, have functions that wind dispelling cold expelling, relaxing muscles and tendons and activating QI and blood in the collateral;For controlling the diseases such as lumbago and skelalgia, numb hand and foot, grain be obstructed.And
And the proteoglycan body in Pleurotus ostreatus has very strong inhibitory action to cancerous cell, can enhancing human body immunity function.Due to Pleurotus ostreatus only
No two nutritive value, it is all the delicious and inexpensive together dish that improves grade on people's dining table for a long time, because it obtains sky
Solely thick disease-resistant health-preserving function, allows Pleurotus ostreatus become very powerful and exceedingly arrogant good merchantable brand on food market, is deeply liked by civic.
The raw material of culture medium of edible fungus mainly has cotton seed hullss, wood flour, Semen Maydis powder etc. at present, swift and violent with mushroom industry
Development, various places all encounter the bottleneck of the restriction industry such as there is lack of raw materials, spurt in prices leap, inquire into Novel editable mushroom medium
Matter, seeking locally cheap cultivation resource has become mushroom industry problem anxious to be resolved.
Eucalyptuss are one of three famous greatly seeds in the world, and Eucalyptus Plantation in Guangxi area, increment, accumulation all occupy the whole nation
First.Eucalyptus Wood can produce substantial amounts of mallee bark in process, because bark bulk density is low, volume big, and point
Cloth is loose, is not easy to collect, store and transport, bark is a kind of high-moisture, high volatile, low grey, low nitrogen, low-sulfur, heating again
Measure low nonflammable material, therefore traditional processing mode many to abandon, to burn or to fill etc. based on plain mode on the spot,
Not only the huge wasting of resources but also serious environment pollution had been caused.If mallee bark can be used for culturing edible fungus, it will turn waste into wealth,
Make full use of local characteristic resources, solve the problems such as shortage of resources, spurt in prices of running in edible fungi development.
Content of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of oyster mushroom culture medium and preparation method thereof, research
Using mallee bark as the oyster mushroom culture medium of primary raw material, obtain aboundresources, cheap cultivation resource.
In order to achieve the above object, the present invention adopts the technical scheme that:
A kind of oyster mushroom culture medium, is formed by the preparation of raw material of following weight portion:25~45 parts of mallee bark, Ramulus Mori 25~40
Part, 5~12 parts of Testa Tritici, 4~10 parts of peanut residue, 7~15 parts of corn cob, 1.7~3 parts of pulverized limestone, 0.6~1.3 part of Gypsum Fibrosum, excessively phosphorus
0.2~0.5 part of sour calcium, 1~2 part of sucrose.
As preferred version, described oyster mushroom culture medium, formed by the preparation of raw material of following weight portion:35 parts of mallee bark,
33 parts of Ramulus Mori, 9 parts of Testa Tritici, 7 parts of peanut residue, 11 parts of corn cob, 2.3 parts of pulverized limestone, 0.9 part of Gypsum Fibrosum, 0.3 part of calcium superphosphate,
1.5 parts of sucrose.
A kind of preparation method of oyster mushroom culture medium, comprises the following steps:
(1) mallee bark of above-mentioned weight portion, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum
It is respectively crushed into fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is molten
Yu Shuizhong pours in compound, sprays water while stirring, and adds pulverized limestone to adjust pH, and windrow ferments;
(3) culture medium fermenting is loaded polythene material thin film barrel bag, pack, then by the culture base-material of pack
Normal-pressure sterilization 10~12h at 100 DEG C, is cooled to 23~28 DEG C, obtains final product oyster mushroom culture medium.
Further, in described step (1), Testa Tritici, peanut residue, Gypsum Fibrosum are respectively crushed into 80 mesh fine powders.
Further, spraying water while stirring in described step (2) to moisture content is 65%~70%.
Further, adjust pH to 7.0~8.0 in described step (2).
Further, in described step (2), windrow ferments 3~6 days.
Further, in described step (3), culture medium is packed 300~400g/ bag.
Compared with prior art, beneficial effects of the present invention:
The primary raw material mallee bark of the present invention, Ramulus Mori are the side-products of Guangxi preponderant dinstinctive industry, and resource is extremely abundant,
Cheap, the nutrient substance contained by culture medium preparing disclosure satisfy that mushroom growth demand, and the Pleurotus ostreatus color and luster turned out is clean
In vain, growing way is dense, a pork thickness, and sarcocyte is tough and tensile, is that the large-scale industrialized production of Pleurotus ostreatus provides guarantee;Not only improve bacterium industry
Flourish, make full use of the resource of Eucalyptuss, mulberry again, increase its process added value, preserve the ecological environment, hence with eucalyptus globuluss
Bark culture Pleurotus ostreatus have broad based growth prospect.
Specific embodiment
With reference to specific embodiment, the present invention is further described.
Embodiment 1
A kind of oyster mushroom culture medium is formed by the preparation of raw material of following weight portion:Mallee bark 35Kg, Ramulus Mori 35Kg, Testa Tritici
10Kg, peanut residue 8Kg, corn cob 7Kg, pulverized limestone 1.7Kg, Gypsum Fibrosum 1Kg, calcium superphosphate 0.3Kg, sucrose 2Kg.
The preparation method of oyster mushroom culture medium, comprises the steps:
(1) mallee bark, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum are respectively crushed into 80
Mesh fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is molten
Yu Shuizhong pours in compound, and spraying water while stirring to moisture content is 65%, and adds pulverized limestone to adjust pH to 7.0, and windrow is sent out
Ferment, every 1d turning 1 time, windrow ferments 3 days;
(3) culture medium fermenting is loaded 23cm × 43cm polythene material thin film barrel bag, pack 300g/ bag, so
Afterwards by normal-pressure sterilization 10h at 100 DEG C of the culture base-material of pack, it is cooled to 25 DEG C, obtains final product oyster mushroom culture medium.
Embodiment 2
A kind of oyster mushroom culture medium is formed by the preparation of raw material of following weight portion:Mallee bark 45Kg, Ramulus Mori 30Kg, Testa Tritici
7Kg, peanut residue 5Kg, corn cob 8Kg, Calx 2.5Kg, Gypsum Fibrosum 1.3Kg, calcium superphosphate 0.2Kg, sucrose 1Kg.
The preparation method of oyster mushroom culture medium, comprises the steps:
(1) mallee bark, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum are respectively crushed into 80
Mesh fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is molten
Yu Shuizhong pours in compound, and spraying water while stirring to moisture content is 68%, and adds pulverized limestone to adjust pH to 7.3, and windrow is sent out
Ferment, every 1d turning 1 time, windrow ferments 4 days;
(3) culture medium fermenting is loaded 23cm × 43cm polythene material thin film barrel bag, pack 320g/ bag, so
Afterwards by normal-pressure sterilization 11h at 100 DEG C of the culture base-material of pack, it is cooled to 23 DEG C, obtains final product oyster mushroom culture medium.
Embodiment 3
A kind of oyster mushroom culture medium is formed by the preparation of raw material of following weight portion:Mallee bark 25Kg, Ramulus Mori 40Kg, Testa Tritici
12Kg, peanut residue 4Kg, corn cob 12Kg, Calx 3Kg, Gypsum Fibrosum 2Kg, calcium superphosphate 0.5Kg, sucrose 1.5Kg.
The preparation method of oyster mushroom culture medium, comprises the steps:
(1) mallee bark, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum are respectively crushed into 80
Mesh fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is molten
Yu Shuizhong pours in compound, and spraying water while stirring to moisture content is 66%, and adds pulverized limestone to adjust pH to 7.6, and windrow is sent out
Ferment, every 1d turning 1 time, windrow ferments 5 days;
(3) culture medium fermenting is loaded 23cm × 43cm polythene material thin film barrel bag, pack 350g/ bag, so
Afterwards by normal-pressure sterilization 12h at 100 DEG C of the culture base-material of pack, it is cooled to 28 DEG C, obtains final product oyster mushroom culture medium.
Embodiment 4
A kind of oyster mushroom culture medium is formed by the preparation of raw material of following weight portion:Mallee bark 40Kg, Ramulus Mori 25Kg, Testa Tritici
5Kg, peanut residue 10Kg, corn cob 15Kg, Calx 3Kg, Gypsum Fibrosum 0.6Kg, calcium superphosphate 0.4Kg, sucrose 1Kg.
The preparation method of oyster mushroom culture medium, comprises the steps:
(1) mallee bark, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum are respectively crushed into 80
Mesh fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is molten
Yu Shuizhong pours in compound, and spraying water while stirring to moisture content is 70%, and adds pulverized limestone to adjust pH to 8.0, and windrow is sent out
Ferment, every 1d turning 1 time, windrow ferments 6 days;
(3) culture medium fermenting is loaded 23cm × 43cm polythene material thin film barrel bag, pack 400g/ bag, so
Afterwards by normal-pressure sterilization 12h at 100 DEG C of the culture base-material of pack, it is cooled to 26 DEG C, obtains final product oyster mushroom culture medium.
Test example:The test that the oyster mushroom culture medium of the present invention affects on Growth of Pleurotus Mycelium
1st, test strain:Pleurotus ostreatus kind is tea 39, and edible fungi research department of institute of microbiology of Guangxi Academy of Agricultural Sciences provides.
2nd, culture medium prescription:
The embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, comparative example.
Comparative example presses " cotton seed hullss 35Kg, Ramulus Mori 33Kg, Testa Tritici 9Kg, peanut residue 7Kg, corn cob 11Kg, pulverized limestone
2.3Kg, Gypsum Fibrosum 0.9Kg, calcium superphosphate 0.3Kg, sucrose 1.5Kg, pH is 7.0~8.0, water content 65%~70% " formula joins
Culture medium processed.
3rd, test method:
With the test tube of 30mm × 200mm, each formula respectively fills 10, and culture medium loading amount is the 1/3 of test tube, sterilizing, cooling
Make isometric inclined-plane, each formula accesses Pleurotus ostreatus tea 39 strain, be placed in 25~28 DEG C of incubator cultures, observe mycelial growth daily
Situation.Record mycelial growth rate, the long potential density of mycelia, mycelia color and luster growing way, it is repeated 3 times.
4th, result of the test:
Table 1 Pleurotus ostreatus mycelial growth situation on different culture media
"+", represents dense degree of mycelia, "+" more, represent that mycelia is denser.
As it can be seen from table 1 nutrient substance contained by the culture medium of the present invention disclosure satisfy that mushroom growth demand, use the present invention
The Pleurotus ostreatus color and luster that culture medium culturing goes out is pure white, growing way is dense, a pork thickness, and sarcocyte is tough and tensile.Although planting effect does not reach Semen Gossypii
The effect of shell, but mallee bark is the side-product of Guangxi preponderant dinstinctive industry, and resource is extremely abundant, cheap, thus, it can be known that
As oyster mushroom culture medium, there is feasibility and practicality using by bark.
The above, be only present pre-ferred embodiments, not the technical scope of the present invention imposed any restrictions, therefore
Any trickle amendment, equivalent variations and modification that every technical spirit according to the present invention is made to above example, all still belong to
In the range of technical solution of the present invention.
Claims (8)
1. a kind of oyster mushroom culture medium is it is characterised in that formed by the preparation of raw material of following weight portion:25~45 parts of mallee bark, Mulberry
25~40 parts of branch, 5~12 parts of Testa Tritici, 4~10 parts of peanut residue, 7~15 parts of corn cob, 1.7~3 parts of pulverized limestone, Gypsum Fibrosum 0.6~
1.3 parts, 0.2~0.5 part of calcium superphosphate, 1~2 part of sucrose.
2. oyster mushroom culture medium according to claim 1 is it is characterised in that formed by the preparation of raw material of following weight portion:Eucalyptus globuluss
35 parts of bark, 33 parts of Ramulus Mori, 9 parts of Testa Tritici, 7 parts of peanut residue, 11 parts of corn cob, 2.3 parts of pulverized limestone, 0.9 part of Gypsum Fibrosum, peroxophosphoric acid
0.3 part of calcium, 1.5 parts of sucrose.
3. the preparation method of oyster mushroom culture medium according to claim 1 and 2 is it is characterised in that comprise the following steps:
(1) mallee bark of above-mentioned weight portion, Ramulus Mori, maize cob meal are broken into disintegrating slag, dry, Testa Tritici, peanut residue, Gypsum Fibrosum are respectively
It is ground into fine powder, standby;
(2) by mallee bark, Ramulus Mori, corn cob disintegrating slag, Testa Tritici, peanut residue, Gypsum Fibrosum, calcium superphosphate mixing, sucrose is dissolved in water
In pour in compound, spray water while stirring, and add pulverized limestone adjust pH, windrow ferment;
(3) culture medium fermenting is loaded polythene material thin film barrel bag, pack, then by the culture base-material 100 of pack
Normal-pressure sterilization 10~12h at DEG C, is cooled to 23~28 DEG C, obtains final product oyster mushroom culture medium.
4. the preparation method of oyster mushroom culture medium according to claim 3 is it is characterised in that Testa Tritici, flower in described step (1)
Raw bran, Gypsum Fibrosum are respectively crushed into 80 mesh fine powders.
5. oyster mushroom culture medium according to claim 3 preparation method it is characterised in that in described step (2) side stirring
While spraying water to moisture content is 65%~70%.
6. the preparation method of oyster mushroom culture medium according to claim 3 is it is characterised in that adjust pH in described step (2)
To 7.0~8.0.
7. oyster mushroom culture medium according to claim 3 preparation method it is characterised in that in described step (2) windrow send out
Ferment 3~6 days.
8. the preparation method of oyster mushroom culture medium according to claim 3 will be it is characterised in that will cultivate in described step (3)
Base packs 300~400g/ bag.
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Cited By (3)
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CN109042079A (en) * | 2018-09-30 | 2018-12-21 | 安徽铜草花现代农业科技有限公司 | A kind of culture base-material for cultivating oyster mushroom |
CN109042074A (en) * | 2018-08-28 | 2018-12-21 | 铜陵盛牛菌业有限责任公司 | A kind of oyster mushroom culture medium and preparation method thereof |
CN113951049A (en) * | 2021-09-03 | 2022-01-21 | 广西南亚热带农业科学研究所 | Culture medium taking macadimia nut peels as raw materials and method for cultivating oyster mushrooms by using culture medium |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109042074A (en) * | 2018-08-28 | 2018-12-21 | 铜陵盛牛菌业有限责任公司 | A kind of oyster mushroom culture medium and preparation method thereof |
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CN113951049A (en) * | 2021-09-03 | 2022-01-21 | 广西南亚热带农业科学研究所 | Culture medium taking macadimia nut peels as raw materials and method for cultivating oyster mushrooms by using culture medium |
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Application publication date: 20170215 |