CN106365701A - Microbial rapid decomposition agent for straw and rapid decomposition method - Google Patents
Microbial rapid decomposition agent for straw and rapid decomposition method Download PDFInfo
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- CN106365701A CN106365701A CN201610667786.1A CN201610667786A CN106365701A CN 106365701 A CN106365701 A CN 106365701A CN 201610667786 A CN201610667786 A CN 201610667786A CN 106365701 A CN106365701 A CN 106365701A
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- microorganism
- quick rotting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Abstract
The invention provides a microbial rapid decomposition agent for straw and a rapid decomposition method, belonging to the field of microbial rapid decomposition technology capable of producing no stink in rapid decomposition of straw. The microbe-dormant powder of the microbial rapid decomposition agent comprises, by weight, 1 to 2 parts of Cladosporium cladosporioides, 8 to 12 parts of Rhodotorula rubra, 8 to 12 parts of Trichosporon mucoides, 7 to 10 parts of Hansenula anomala and 27 to 32 parts of Bacillus subtilis. The rapid decomposition method comprises the following steps: (1) preparation of the microbial rapid decomposition agent; (2) activation and dilution; and (3) sprinkling of the bacterial liquid of the microbial rapid decomposition agent. The microbial rapid decomposition agent and the rapid decomposition method have the advantages that 70% of wheat straw is decomposed in 45 days and no stink is emitted; 70% of corn straw is decomposed in 35 days and no stink is emitted; 70% of rice straw is decomposed in 25 days and no stink is emitted; and gases with stink like hydrogen sulfide, acetamide and ammonia gas are not produced during decomposition.
Description
Technical field
The invention belongs to having disengaged from soil it is impossible to the straw of regrowth carries out the rotten technology of microorganism speed, particularly relating to
And during straw decomposition, do not produce the microorganism speed corruption technology of stink.
Background technology
China's annual agricultural crop straw amount is huge, mainly cellulose (polysaccharide macromolecular material), hemicellulose, on a small quantity
Also have protein in the seed omitted, straw degradative also field can living resources recycle, solve carelessly to abandon again or field is burnt
Burn the huge waste causing environmental pollution.Straw is no controlled and is burned the severe haze weather brought, and is just badly influencing us
Often live.
Chinese patent " straw putrid fermentation agent " 201510574578.2 is a kind of new zymocyte being cultured out,
Its training method is certainly first, will celebrate curly hair yeast-like fungi, Semen Maydiss 50%, water 50% fermentation culture；Secondly, Hypocrea virens are trained
Support, take rotten wood unqualified 35%, wheat bran 50%, water 15% fermentation culture；Finally, the culture of funguses, adds Herba polygoni hydropiperis 30%,
White sugar 30%, water 20%, fermentation culture.
A kind of Chinese patent " microorganism quick rotting agent and preparation method thereof " 200710049161.x microorganism quick rotting agent, including
Yeast and cellulose-decomposing bacteria are it is characterised in that the weight proportion of described microbial components is: 30～60 parts of yeast, fiber divides
40～70 parts of bacterium of solution.
Chinese patent " decomposing microbial inoculum of a kind of bacillus subtilises h4 and its preparation " 201410519541.5 present invention is public
Decomposing microbial inoculum, the application of decomposing microbial inoculum of a kind of bacillus subtilises h4 and its preparation are opened, described decomposing microbial inoculum is by short bud
Spore bacillus h3 dormant state powder, bacillus subtilises h4 dormant state powder, bacillus subtilises h8-3 dormant state powder and starch are with weight
Constitute than for 1: 1: 1: 97；The present invention provides one plant of new strains -- bacillus subtilises h4, using the bacterium of becoming thoroughly decomposed of this bacterial strain preparation
Agent have high temperature resistant, extracellular proteinase, Digestive Enzyme with the advantage of high-efficiency fermenting animal class garbage, using described decomposing microbial inoculum heap
Fertilizer has the advantages that environmental pollution is little, economy and facility, and the product after compost treatment, can be used as organic fertilizer through subsequent treatment
Reach the purpose turning waste into wealth.
Content of the invention
It is an object of the invention to provide acceleration straw decomposition, do not produce the stalk microbe quick rotting agent of stink, and its use
The microorganism quick rotting agent of this straw carries out the quick decomposition method of odorless to straw.
The technical scheme is that
The microorganism quick rotting agent of straw, including bud dendritic branch spore, rhodothece rubra, viscosity trichosporon, Hansenula anomala
With bacillus subtilises it is characterised in that: described dormant state powder microorganism wt ratio is:
Bud dendritic branch spore 1-2 part；Rhodothece rubra 8-12 part；Viscosity trichosporon 8-12 part；
Hansenula anomala 7-10 part；Bacillus subtilises 27-32 part.
This quick rotting agent is mainly used in the quick decomposition of straw odorless.
Bud dendritic branch spore (cladosporium cladosporioides) produces cellulase, the fiber in decomposition straw
Element and hemicellulose.
Rhodothece rubra (rhodotorula rubra) produces acid protease, the protein in decomposition straw.
Viscosity trichosporon (trichosporon mucoides) produces alkaline protease, the protein in decomposition straw.
Hansenula anomala (hansenula anomala) produces neutral protease, the protein in decomposition straw.
Bacillus subtilises (bacillus subtilis) produce based on pectase, also have cellulase and protease, rotten
Based on pectin in solution straw.
No mutual inhibitory action between various microorganisms in this microorganism quick rotting agent.
In the protein of straw, there are acidic protein, alkaline protein, neutral protein, thus rotten in this microorganism speed
Select the bacterium producing acid protease, the bacterium producing alkaline protease, the bacterium of generation neutral protease in agent, made various albumen
Matter can be by the decomposition of aerobic bacteria or aerobic suspicion bacterium odorless.Both the purpose having produced protease from these three is to carry out no
The decomposition straw of stink.And select the bacterium producing cellulose in the present invention and produce the bacterium of pectase just for thin in straw
Intercellular supporter, both the cellulose on cell wall and interstitial were good by decomposition execution, made straw lose three-dimensional shape profile
And subside, volume-diminished；Material molecule diminishes to be conducive to being quickly entered soil by rotten material becomes fertilizer.
This quick rotting agent to straw odorless the reason quick decomposition can be: the bacterium that the present invention selects is all aerobic bacteria or aerobic
The property disliked bacterium, the enzyme that these bacterium produce is not to producing the odorous gas such as hydrogen sulfide, acetamide and ammonia during straw decomposition.Thus
Reach i.e. decomposition straw, and the purpose of odorless.
The microorganism dormancy state powder part by weight of the microorganism quick rotting agent of the preferred straw of the present invention is:
1 part of bud dendritic branch spore；8 parts of rhodothece rubra；8 parts of viscosity trichosporon；
8 parts of Hansenula anomala；30 parts of bacillus subtilises.
The microorganism quick rotting agent of straw carries out the method for fast rotting it is characterised in that comprising the following steps to straw:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
Bud dendritic branch spore 1-2 part；Rhodothece rubra 8-12 part；Viscosity trichosporon 8-12 part；
Hansenula anomala 7-10 part；Bacillus subtilises 27-32 part；
In 1 gram of dormant state powder microorganism quick rotting agent, various active micro organism quantities are:
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution.
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
On jin disengaging plant, the straw departing from soil, can not growing.It carries out microorganism speed corruption effect and is shown in Table 1 to straw.
To various stalk microbes speed corruption effect table 1
With in table 1,45 days wheat stalks of test group rot 70%, odorless；35 days corn straw rotten 70%, odorless
Taste；25 days rice straws rotten 70%, odorless.Matched group begins with a small amount of stink for 35 days, lose within 60 days have rotten.
(1) test group: in straw corn trials group, in 35 days 1 gram of rotten corn straw, have 160,000,000 aerobic and suspicion
Property bacterium, has 3,000,000 anaerobe.Because major part is aerobic and the property disliked bacterium, fraction is anaerobe；Aerobic and suspicion oxygen bacterium speed is rotten
Straw does not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia；And fraction anaerobe speed corruption straw generation hydrogen sulfide,
Acetamide and ammonia are few.So having reached the purpose of the present invention: so that the straw decomposition time is accelerated than naturalness decomposition time, and
And odorless or only a small amount of stink.
(2) matched group: in the multiple mixed straw of matched group, in 35 days 1 gram of rotten corn straw, have 4,000,000 aerobic and
The property disliked bacterium, has 12,200,000 anaerobe.Because major part is anaerobe, fraction is aerobic and the property disliked bacterium；Substantial amounts of anaerobe is produced
Raw hydrogen sulfide, acetamide and ammonia.
It can be seen that, the present invention has substantially excellent quickening speed rotten than matched group, and the technique effect of odorless.
The various strains of the present invention are all purchased from Institute of Microorganism, Academia Sinica.
Each strain that the present invention buys is obtained each strain of product respectively with following " level Four fermentation " technique
Microorganism dormancy state powder is obtained each strain dormant state powder, then these each strain dormant state powder is pressed the weight ratio of formula
Mix homogeneously, obtains the stalk microbe quick rotting agent of the present invention.
" level Four fermentation " technique of each strain is as follows:
(1) the 1st grade of seed selection culture
In gnotobasiss, the culture medium of agar slant will be accessed after a certain strain dormant state powder aquation bought
In, after strain purification, bacterial screening, test tube inoculation and shake-flask culture, bacterium solution is made by liquid fermentation.Described inclined-plane bacterium
Kind culture: the slant culture of strain requires slant strains must not be mixed with any miscellaneous bacteria and phage, cultivates at 28 DEG C of constant temperature.
This seed culture is to select energetic thalline.
(2) the 2nd grades of seed amplification culture
1st grade of seed bacterium solution amplification culture is made the strain liquid of 1000-2000ml.
(3) 3rd level seed tank fermentation culture
This step inoculates the 2nd grade of zymocyte liquid according to 5% inoculum concentration, makes 500l strain liquid.
(4) the 4th grades of fermentor cultivation
In this step, the inoculum concentration according to 10% inoculates 3rd level zymocyte liquid, makes the fermentation liquid of 5000l.Then by the 4th
Level fermentation liquid is dried to dormant state powder.
Advantages of the present invention: 45 days wheat stalks rotten 70%, odorless；35 days corn straw rotten 70%, odorless；
25 days rice straws rotten 70%, odorless.
From the aerobic of energy Fast-propagation in straw and the property disliked bacterium, the anaerobe in suppression straw, make straw aerobic
And the property the disliked quick decomposition of bacterium, decomposition do not produce the odorous gas such as hydrogen sulfide, acetamide and ammonia.
The present invention is had from the aerobic of decomposition straw and the property disliked bacterium: produces the bud dendritic branch spore of cellulase, produces albumen
The rhodothece rubra of enzyme, viscosity trichosporon and Hansenula anomala, produce the bacillus subtilises cooperation based on pectase.
Specific embodiment
Embodiment 1, the microorganism quick rotting agent of rice straw enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is as follows:
(1) the 1st grade of seed selection culture
In gnotobasiss, the culture medium of agar slant will be accessed after a certain strain dormant state powder aquation bought
In, after strain purification, bacterial screening, test tube inoculation and shake-flask culture, bacterium solution is made by liquid fermentation.Described inclined-plane bacterium
Kind culture: the slant culture of strain requires slant strains must not be mixed with any miscellaneous bacteria and phage, cultivates at 28 DEG C of constant temperature.
This seed culture is to select energetic thalline.
(2) the 2nd grades of seed amplification culture
1st grade of seed bacterium solution amplification culture is made the strain liquid of 1000-2000ml.
(3) 3rd level seed tank fermentation culture
This step inoculates the 2nd grade of zymocyte liquid according to 5% inoculum concentration, makes 500l strain liquid.
(4) the 4th grades of fermentor cultivation
In this step, the inoculum concentration according to 10% inoculates 3rd level zymocyte liquid, makes the fermentation liquid of 5000l.Then by the 4th
Level fermentation liquid is dried to dormant state powder.
The microorganism quick rotting agent of straw and the method that rice straw is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
1 part of bud dendritic branch spore；8 parts of rhodothece rubra；8 parts of viscosity trichosporon；
8 parts of Hansenula anomala；30 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the rice straw of plant.
Embodiment 2, the microorganism quick rotting agent of rice straw enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism quick rotting agent of straw and the method that rice straw is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
1 part of bud dendritic branch spore；8 parts of rhodothece rubra；8 parts of viscosity trichosporon；
7 parts of Hansenula anomala；27 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the rice straw of plant.
Embodiment 3, the microorganism quick rotting agent of corn straw enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism quick rotting agent of straw and the method that corn straw is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
2 parts of bud dendritic branch spore；12 parts of rhodothece rubra；12 parts of viscosity trichosporon；
10 parts of Hansenula anomala；32 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the corn straw of plant.
Embodiment 4, the microorganism quick rotting agent of corn straw enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism quick rotting agent of straw and the method that corn straw is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
1.5 parts of bud dendritic branch spore；10 parts of rhodothece rubra；9 parts of viscosity trichosporon；
10.0 parts of Hansenula anomala；30.0 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the corn straw of plant.
Embodiment 5, the microorganism quick rotting agent of wheat stalk enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism quick rotting agent of straw and the method that wheat stalk is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
2 parts of bud dendritic branch spore；11 parts of rhodothece rubra；10 parts of viscosity trichosporon；
8 parts of Hansenula anomala；28 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the wheat stalk of plant.
Embodiment 6, the microorganism quick rotting agent of wheat stalk enter the rotten method of scanning frequency
Prepare every kind of microorganism dormancy state powder first respectively, then press formula proportion and every kind of microorganism dormancy state powder is mixed system
Obtain the microorganism quick rotting agent of the present embodiment.
" level Four fermentation " technique of every kind of microorganism dormancy state powder is same as embodiment 1.
The microorganism quick rotting agent of straw and the method that wheat stalk is entered with scanning frequency corruption, comprise the following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, be obtained
Microorganism quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
2 parts of bud dendritic branch spore；10 parts of rhodothece rubra；12 parts of viscosity trichosporon；
7 parts of Hansenula anomala；29 parts of bacillus subtilises.
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water and is slowly stirred under 25-35 degrees celsius
Mixing 24 hours, makes microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted with 100 kilograms
Become microorganism quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 100-400 public
Jin departs from the wheat stalk of plant.
Claims (3)
1. the microorganism quick rotting agent of straw, including bud dendritic branch spore, rhodothece rubra, viscosity trichosporon, Hansenula anomala and
Bacillus subtilises it is characterised in that: described dormant state powder microorganism wt ratio is:
Bud dendritic branch spore 1-2 part；Rhodothece rubra 8-12 part；Viscosity trichosporon 8-12 part；
Hansenula anomala 7-10 part；Bacillus subtilises 27-32 part.
2. stalk microbe quick rotting agent according to claim 1 it is characterised in that: described dormant state powder microorganism wt
Ratio is:
1 part of bud dendritic branch spore；8 parts of rhodothece rubra；8 parts of viscosity trichosporon；
8 parts of Hansenula anomala；30 parts of bacillus subtilises.
3. straw is entered with the rotten method of scanning frequency it is characterised in that including with the stalk microbe quick rotting agent described in claim 1 or 2
Following steps:
(1) prepare microorganism quick rotting agent: various microorganism dormancy state powder are pressed the part by weight mix homogeneously of formula, micro- life is obtained
Thing quick rotting agent, the part by weight of its various microorganism dormancy state powder formula is,
Bud dendritic branch spore 1-2 part；Rhodothece rubra 8-12 part；Viscosity trichosporon 8-12 part；
Hansenula anomala 7-10 part；Bacillus subtilises 27-32 part；
(2) activate and dilute: 5.0 grams of microorganism quick rotting agents are added 500ml water under 25-35 degrees celsius, is slowly stirred mixing
24 hours, make microorganism quick rotting agent activating solution；Again activated microorganism quick rotting agent activating solution is diluted to micro- with 100 kilograms
Biological quick rotting agent bacterium solution；
(3) spray microorganism quick rotting agent bacterium solution: 100 kilograms of microorganism quick rotting agent bacterium solution are uniformly sprayed at 1000-400 kilogram and take off
On the straw of plant.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108017428A (en) * | 2017-12-12 | 2018-05-11 | 长春工业大学 | A kind of straw decomposition microbial inoculum and its application |
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