CN106361967A - Bitter gourd, agrimonia pilosa ledeb and radix ophiopogonis extract and preparation method and application thereof - Google Patents

Bitter gourd, agrimonia pilosa ledeb and radix ophiopogonis extract and preparation method and application thereof Download PDF

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CN106361967A
CN106361967A CN201610879054.9A CN201610879054A CN106361967A CN 106361967 A CN106361967 A CN 106361967A CN 201610879054 A CN201610879054 A CN 201610879054A CN 106361967 A CN106361967 A CN 106361967A
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extract
radix ophiopogonis
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parts
herba agrimoniae
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郑国�
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Hubei Sanxia Shennong Biological Science And Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The invention belongs to the technical field of health-care products and particularly relates to a bitter gourd, agrimonia pilosa ledeb and radix ophiopogonis extract and a preparation method and application thereof. The extract is prepared from, by weight, 20-30 parts of bitter gourds, 15-30 parts of agrimonia pilosa ledeb and 10-20 parts of radix ophiopogonis. The preparation method of the bitter gourd, agrimonia pilosa ledeb and radix ophiopogonis extract includes the following steps that raw material components are weighed and smashed in sequence through a traditional Chinese medicine smashing machine; the smashed radix ophiopogonis is subjected to ultrasonic-microwave synergistic extraction, and agrimonia pilosa ledeb is heated and subjected to reflux extraction by means of purified water; the bitter gourds are extracted through cellulase and microwaves; the extracted materials are subjected to high-speed dispersion, emulsion and pressurized extraction, and bamboo vinegar is processed through ultrasound and electric fields. Tablets prepared from the extract have an obvious effect on influenza viruses and blood glucose reduction treatment, and have no toxic and side effects on bodies. When a patient uses the tablets, drug dependency is not generated. The traditional Chinese medicine is low in price and can be accepted by ordinary people.

Description

A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract and its preparation method and application
Technical field
The invention belongs to health product technology field, especially relate to a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract and its preparation Methods and applications.
Background technology
Influenza viruss (influenza virus), are the representatives of orthomyxoviridae family (orthomyxoviridae) Kind, abbreviation influenza virus, including human influenza viruses and animal influenza virus, human influenza viruses are divided into first (a), second (b), third (c) Three types, are the pathogen of influenza (influenza).Wherein influenza A virus antigen easily morphs, and repeatedly causes the world Property is very popular.For example in 1918～1919 years be very popular, the whole world at least 20,000,000～40,000,000 people dies from influenza；B-mode Influenza virus are also stronger to human pathogenic, but people also do not find that Influenza B viruss caused worldwide big stream OK；Influenza virus C only causes the unconspicuous or slight upper respiratory tract infection of the mankind, seldom causes popular.Influenza A disease Poison obtains in 1940 in separation success in 1933, Influenza B viruss, and influenza virus C was just successfully separated until 1949. Typically adopt western medicine for influenza virus treatment, Chinese medicine preparation less, Western medicine Small side effects compared by Chinese medicine, have very big Exploitation space.
Content of the invention
Based on this, the present invention provides a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract and its preparation method and application, and effect becomes Code insurance stays abundant, favourable absorption of human body.
Technical solution of the present invention is:
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
20～30 parts of Fructus Momordicae charantiae, 15～30 parts of Herba Agrimoniae, 10～20 parts of Radix Ophiopogonis.
Preferably, described extract also includes following parts by weight of component:
8～13 parts of bamboo vinegar liquid.
The method making described Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, the method comprising the steps of:
1) weigh raw material components, successively raw material is pulverized respectively with Chinese medicine grinder, each grinding time 2-10 divides Clock, is pulverized with super micron mill after crossing 100 mesh sieves again, standby；
2) by described step 1) pulverize after the organic solvent adding its 8～15 times of weight Radix Ophiopogonis, carry out ultrasonic-microwave association With extracting, filter to get filtrate, remove filtering residue concentrating under reduced pressure, drying, dried object is pulverized, obtains material 1；
3) by described step 1) pulverize after Herba Agrimoniae, with pure water heating and refluxing extraction 2-3 time of 5-10 times of weight, conjunction And lixiviating solution, by gained lixiviating solution concentrating under reduced pressure, dry, pulverize, obtain material 2；
4) by described step 1) pulverize after Fructus Momordicae charantiae add distilled water, with cellulose treatment, filter and retain filtering residue, then to Wherein add distilled water, microwave treatment, then heating in water bath, filter to get filtrate, concentrating under reduced pressure, dry, pulverize, obtain material 3；
5) material 1, material 2, material 3 are mixed in proportion, smash, add the water of mixture identical weight, put at a high speed Dispersion emulsifying machine is processed, and obtains high speed dispersion and emulsion thing；
6) pressurised extraction: by step 5) process after emulsion, put in pressure pan, add emulsion identical weight Water, pressurization and then recovery normal pressure, filter, obtain extracting solution, and be condensed into extractum, and concentrating under reduced pressure, drying obtain extract；
7) by bamboo vinegar liquid with combination of ultrasound electric field treatment, and it is condensed into extractum, concentrating under reduced pressure, drying, baking temperature 50-60 DEG C, to moisture below 6%, pulverize, cross 80～90 mesh sieves, obtain bamboo vinegar extract；
8) by described step 6) extract that obtains, described step 7) mixing of bamboo vinegar extract；
Complete the making of extract.
Preferably, described step 2) organic solvent is the ethanol that volumetric concentration is 65%～85%, ultrasonic-microwave cooperating carries The condition taking is: power 30～70w, frequency 12～30khhz, carries out ultrasonic-microwave cooperating and extracts 500～1200s, described Alkali liquor is sodium hydrate aqueous solution.
Preferably, described step 2) material 1 makes and also includes: carries out the filtering residue after ultrasonic-microwave cooperating extracts and adds it The sodium hydrate aqueous solution of 30～50 times of weight, adjusting ph is 7～9, and temperature is 70～95 DEG C, carries out extracting 15～35min, mistake Filter resulting solution, the filtrate extracted with ultrasonic-microwave cooperating is mixed, and dried object is pulverized, obtained by concentrating under reduced pressure, drying Material 1.
Preferably, methods described also include raw material Herba Agrimoniae carry out before pulverizing the efficacy of a drug fermentation pretreatment, its processing method For: by Herba Agrimoniae freezing processing, then steam treatment, be dried form efficacy of a drug fermentation Herba Agrimoniae and carry out described step 1 again) at pulverizing Reason.
It is further preferred that -25～-10 DEG C of described freezing processing temperature, freezing processing 3-7h, described vapor (steam) temperature is 60-75 DEG C, steam pressure is 0.1-0.25mpa, continues 30～35 minutes.
Preferably, described step 4) pulverize after Fructus Momordicae charantiae add distilled water, concentration is controlled to 1:4～12g/ml, uses fiber Plain ferment treatment 8～20min, mass percentage concentration in purification Fructus Momordicae charantiae distilled water for the cellulase is 0.4%～0.9%, filters Retain filtering residue, then 1:15～35 in mass ratio are added thereto to distilled water, 600w～1200w microwave treatment 3～8min, then 50 ～70 DEG C of heating in water bath 2.5～5h.
Preferably, described step 5) emulsifying rotating speed 2000-3000r/min, time 10-20min.
Preferably, described step 6) it is forced into 3.5～4kg/cm2, and keep 10-15min, then often recover in 1-3s Pressure.
Preferably, described step 7) combination of ultrasound electric field treatment method is: first with frequency 40～60khz, supersound process 5～ 20min, then the Electric Pulse Treatment 5～10min with intensity 5～10kv/cm.
The application in treatment influenza virus of described extract or described method.
Radix Ophiopogonis, mainly glycosides containing dwarf lilyturf, steroidal saponin, alkaloid, sitosterol, glucose, aminoacid, vitamin etc., had Resisting fatigue, removing free radical, raising cellular immune function and hypoglycemic effect.In addition, having calmness, hypnosis, the anti-heart Radix Ophiopogonis Myocardial ischemia, arrhythmia, antitumor etc. act on, and especially have many-sided effect to enhancement senior health and fitness.Additionally, Radix Ophiopogonis is also There is promotion islet cell function to recover, increase hepatic glycogen, drop hypoglycemic effect.
Herba Agrimoniae: latin name agrimoniae herba, bitter in the mouth, puckery, mild-natured, GUIXIN, Liver Channel, there is astringing to arrest bleeding, only Effect of dysentery parasite killing, cures mainly the card of various bleedings, for example: haematemesis, hematuria, have blood in stool, metrorrhagia, spitting of blood, epistaxis, dysentery, labor The de- power of wound, carbuncle, traumatic injury, wound hemorrhage etc. is demonstrate,proved.
Fructus Momordicae charantiae: heat-clearing and toxic substances removing, skin care skin care, blood sugar lowering, blood-nourishing liver nourishing, Radix Momordicae Charantiae, rattan, leaf and fruit: bitter, tremble with fear.Heat clearing away Removing toxic substances, improving eyesight.For heatstroke heating, toothache, have loose bowels, dysentery, have blood in stool.
Bamboo vinegar is through pyrogenous natural valuable elite by bamboo wood, and it contains more than 200 kind of natural and organic ingredients and mineral Matter, has that antiphlogistic antibacterial, insect protected be fresh-keeping, a smelly eliminating beauty treatment and other effects.
When extract of the present invention is used as medicine, can directly use, or use in the form of a pharmaceutical preparation.Described medicine Preparation contains the present invention-extract of therapeutically effective amount, and remaining is pharmaceutically acceptable, nontoxic to humans and animals and inertia Pharmaceutical acceptable carrier and/or excipient.
Described pharmaceutical acceptable carrier or excipient are to be selected from solid, semi-solid and liquid diluent, filler for one or more And pharmaceutical preparation adjuvant.The pharmaceutical preparation of the present invention is used in the form of per weight dose.Extract of the present invention can Patient in need for the treatment of is applied to by form that is oral or injecting.During for being administered orally, tablet, slow releasing tablet, control can be made into Release piece, capsule, drop pill, micropill, suspensoid, Emulsion, powder or granule, oral liquid etc.；During for injecting, can be made into sterilizing Aqueouss or oily solution, aseptic powder injection, liposome or Emulsion etc..
The present invention has the beneficial effect that:
1st, the extract infected by influenza that the present invention provides, blood sugar lowering therapeutic effect are obvious, and secondary work nontoxic to body With patient does not produce dependence feeling using rear to medicine, and this Chinese medicine price is relatively low, is that ordinary people is acceptable；Can keep away Exempt to take the multiple complications of Western medicine initiation；Secondly, there is taking convenience, easily storage and portable advantage, and biological profit Expenditure significantly improves than ordinary preparation, and the life taste for adapting to allegro life requirement and continuous lifting now provides again One easily route of administration.
2nd, it is directed to different raw materials, using different extracting modes, strive for that extraction efficiency maximizes, and to the thing after extracting Material carries out concentrating under reduced pressure, dried, keeps active component.
4th, the present invention adopt HIGH PRESSURE TREATMENT, under high pressure, its result be protein denaturation, starch gelatinization, enzyme inactivation, micro- Biological death, does not affect on nutritional labeling etc., and pressure seldom, and is reduced to 0 so that living by moment by nutrition loss Property material is isolated, and compared to existing technology, effect is good, and the time is short；
5th, material is carried out with emulsifying, the rotor of high speed dispersion emulsifying machine, in high-speed rotation, produces very big centrifugation Power makes processed material spray from the elongated cuts (slit) of rotation, and under the collective effect of stator, makes processed Material is subject to both sides active force: forces it through the positive force of slit and the resistance stoping it from passing through.Meanwhile, it is processed Material when spraying from slit, be also subject to the shear action of slit, and the rotation being produced by turning stator relative motion cut Shear force makes material obtain homogenizing, emulsifying in the presence of multi-acting force.It is narrow, all that the material processing has particle distribution range Evenness is good, no dead angle, the feature by dispersion and emulsion for the material 100%.
6th, the present invention passes through combination of ultrasound electric field treatment, and the harmful components such as the aldehyde in bamboo vinegar liquid are removed, by bamboo vinegar liquid plus Enter in tea, the effect of antibiotic and sterilizing sterilization can be played, the composition beneficial to human body can be retained simultaneously in tea.The present invention can Understand interior free yl rubbish, delay to enhance metabolism, heat clearing away blood sugar lowering, no added.
8th, for extracting method Radix Ophiopogonis, under microwave electromagnetic field action, these orientation movements are with the frequency of billions of times per second Rate is continually changing, and causes strenuous exercise and the collision friction of molecule, thus producing heat, reaching electric energy and being converted in medium Heat energy.Microwave heating is dielectric material own loss electric field energy and generates heat.Therefore microwave heating is than traditional hot water heating To be beneficial to the physicochemical property keeping extracting product efficiently, in short-term.Ultrasound wave can improve biomembranous permeability, using cavitation effect The extraction effect of Fructus Momordicae charantiae should be improved.Extracted as Main Means using ultrasonic shearing force and-microwave cooperating, it is to avoid long-time Heating and high energy, thus simplifying production technology and cost, safety and environmental protection efficiency high.For ultrasonic-microwave extraction Filtering residue afterwards carries out staged care again, fully extracts functional components Radix Ophiopogonis.
9th, in Fructus Momordicae charantiae extraction process, cellulase is to the degraded of Fructus Momordicae charantiae cell wall and microwave cooperating extraction process technology Process, have impact on the impact to functional components dissolution and physicochemical property of the effect degree and microwave heating of cellulase.In addition, Neutral protease protein degradation matter, dialysis also have impact on yield and the purity of product except small molecular weight impurity.In general, using this Production method obtains product physicochemical property and does not change a lot, and has physiologically active, and yield and purity are all higher.Fiber Element is the main component of plant cell wall.Cellulase biodegradable fiber element, it destroys the network structure of cell wall so as to content Thing discharges as much as possible.Add cellulase therefore in extraction process, the technical process being not only extraction is more warm With, and so that the yield of Fructus Momordicae charantiae functional components and content is all significantly improved.Shearing force and microwave using cellulase Collaborative extract as Main Means, it is to avoid long heating and high energy, thus simplifying production technology and cost, Safety and environmental protection efficiency high.
10th, the present invention passes through combination of ultrasound electric field treatment, and the harmful components such as the aldehyde in bamboo vinegar liquid are removed, by bamboo vinegar liquid plus Enter in Chinese medicine, antibiotic and sterilizing sterilization, the effect of antiviral can be played, retain the composition beneficial to human body, the present invention simultaneously Interior free yl rubbish can be understood, delay to enhance metabolism, no added.
Specific embodiment
To further illustrate the present invention with reference to embodiment, but the scope of protection of present invention is not limited to implement The scope of example statement.
Embodiment 1
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
20～30 parts of Fructus Momordicae charantiae, 15～30 parts of Herba Agrimoniae, 10～20 parts of Radix Ophiopogonis.
Embodiment 2
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
20 parts of Fructus Momordicae charantiae, 15 parts of Herba Agrimoniae, 10 parts of Radix Ophiopogonis.
Embodiment 3
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
30 parts of Fructus Momordicae charantiae, 30 parts of Herba Agrimoniae, 20 parts of Radix Ophiopogonis.
Embodiment 4
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
25 parts of Fructus Momordicae charantiae, 20 parts of Herba Agrimoniae, 15 parts of Radix Ophiopogonis.
Embodiment 5
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
20 parts of Fructus Momordicae charantiae, 15 parts of Herba Agrimoniae, 10 parts of Radix Ophiopogonis, 8 parts of bamboo vinegar liquid.
The method making described Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, the method comprising the steps of:
1) weigh raw material components, successively raw material is pulverized respectively with Chinese medicine grinder, each grinding time 2 minutes, Pulverized with super micron mill again after crossing 100 mesh sieves, fineness is up to 0.006～0.5 μm, standby；
2) by described step 1) pulverize after the organic solvent adding its 8 times of weight Radix Ophiopogonis, carry out ultrasonic-microwave cooperating and carry Take, filter to get filtrate, remove filtering residue concentrating under reduced pressure, drying, dried object is pulverized, obtains material 1；
3) by described step 1) pulverize after Herba Agrimoniae, with pure water heating and refluxing extraction 2-3 time of 5 times of weight, merging Lixiviating solution, by gained lixiviating solution concentrating under reduced pressure, dry, pulverize, obtains material 2；
4) by described step 1) pulverize after Fructus Momordicae charantiae add distilled water, with cellulose treatment, filter and retain filtering residue, then to Wherein add distilled water, microwave treatment, then heating in water bath, filter to get filtrate, concentrating under reduced pressure, dry, pulverize, obtain material 3；
5) material 1, material 2, material 3 are mixed in proportion, smash, add the pure water of mixture identical weight, put into High speed dispersion emulsifying machine is processed, and obtains high speed dispersion and emulsion thing；
6) pressurised extraction: by step 5) process after emulsion, put in pressure pan, add emulsion identical weight pure Water purification, pressurization and then recovery normal pressure, filter, obtain extracting solution, and be condensed into extractum, and concentrating under reduced pressure, drying obtain extract；
7) by bamboo vinegar liquid with combination of ultrasound electric field treatment, and it is condensed into extractum, concentrating under reduced pressure, drying, 50 DEG C of baking temperature, To moisture below 6%, pulverize, cross 80 mesh sieves, obtain bamboo vinegar extract；
8) by described step 6) extract that obtains, described step 7) mixing of bamboo vinegar extract；
Complete the making of extract.
Described step 2) organic solvent is the ethanol that volumetric concentration is 65%, the condition that ultrasonic-microwave cooperating extracts is: work( Rate 30w, frequency 12khhz, carry out ultrasonic-microwave cooperating and extract 500s, described alkali liquor is sodium hydrate aqueous solution.
Described step 2) material 1 makes and also includes: carries out the filtering residue after ultrasonic-microwave cooperating extracts and adds its 30 times of weight Sodium hydrate aqueous solution, adjust ph be 7, temperature be 70 DEG C, carry out extract 15min, filter resulting solution, with ultrasonic-microwave The collaborative filtrate mixing extracted, concentrating under reduced pressure, drying, dried object is pulverized, is obtained material 1.
Methods described also includes raw material Herba Agrimoniae and carries out efficacy of a drug fermentation pretreatment before pulverizing, and its processing method is: by celestial being Crane grass freezing processing, then steam treatment, be dried formed the efficacy of a drug fermentation Herba Agrimoniae carry out described step 1 again) pulverization process.
- 25 DEG C of described freezing processing temperature, freezing processing 3h, described vapor (steam) temperature is 60 DEG C, and steam pressure is 0.1mpa, Continue 30 minutes.
Described step 5) emulsifying rotating speed 2500r/min, time 15min.
Described step 6) it is forced into 3.5kg/cm2, and keep 10min, in 1s, then recover normal pressure.
Described step 7) combination of ultrasound electric field treatment method is: first with frequency 40khz, supersound process 5min, then use intensity The Electric Pulse Treatment 5min of 5kv/cm.
The test of the present embodiment extract pharmacology infected by influenza
First, experiment material
1st, cell strain and virus: Testis et Pentis Canis passage cell (madin darby canine kidney, mdck), influenza A Virus (help anti-90-15), Influenza B viruss (help anti-97-13), Flu virus pr8 are purchased from China prevention doctor Ke Yuan institute of viruses, after virus passes on through Embryo Gallus domesticus, -70 DEG C of cryogenic refrigerators save backup.
2nd, sample and reagent: the inventive method obtains extract and makes piece agent.Positive control drug ribavirin tablet, 0.1g/ piece, Chinese medicines quasi-word h20033538, Jiangxi Huiren Pharmaceutical Co., Ltd produces, lot number: 1411025.
Dmem fluid medium, lot number: 701998, gibco companies；Cell culture fluid, the dmem containing 10% hyclone Fluid medium.Maintaining liquid, in addition to without hyclone, other all same cell culture fluids.Washing liquid is containing 2% dual anti-sulfate chain The mem culture fluid of mycin and kanamycin sulfate.
3rd, animal: cleaning grade Kunming mouse 120, weight 16-20g, male and female half and half, by Xinjiang Animal Experimental Study Center provides, animal quality certification number: scxk (new) 2013-0002.
2nd, method and result
1st, antiviral study in vitro
1) measure the toxicity to mdck cell
Extract maintaining liquid is taken to be made into the stock solution of 5mg/ml, the stock solution that ribavirin is made into 4mg/ml uses maintaining liquid dilute again It is interpreted into 1:2,1:4,1:8,1:16,1:32,1:64,1:128,1:256 totally 8 concentration, be added to inoculation mdck cell and grown up to In 96 well culture plates of monolayer, 100 μ l/ holes, every 4 multiple holes of dilution factor, set cell controls simultaneously.By culture plate put 37 DEG C, 5% co2Cultivate in incubator, daily observation of cell growing state, observe 4d, determine that cell occurs without the minimum dilution of obvious regression Multiple is maximal non-toxic concentration tc0, calculate 50% toxic concentration tc by reed-muench method50.It is shown in Table 1.
Table 1 the present embodiment extract cytotoxicity test result
Sample Stock solution (mg/ml) tc0(μg/ml) tc50(μg/ml)
The present embodiment extract 5 310.5 1350.0
Ribavirin 4 123.0 1832.0
2) impact of samples for viral cytopathogenic effect (cpe)
Take inoculation mdck cell and grown up to the culture plate of monolayer, draw culture fluid, influenza A viruss and influenza B Virus inoculates 100 times of median tissue infection concentration (tcid respectively50) virus liquid 100 μ l, put 35 DEG C, 5%co2Inhale in incubator After attached 1h, inhale and abandon virus liquid, wash cell surface 2 times with washing liquid, if 14 groups, respectively cell controls group, virus control group, profit Ba Weilin takes 61.5 μ g/ml and following 52 times of dilution factors (32,16,8,4,2.0 μ g/m l) totally 6 concentration administration groups, extractions Thing tc0(310.5 μ g/ml) and following 52 times of dilution factors (155,77.5,38.8,19.4,9.7 μ g/ml) the giving of totally 6 concentration Medicine group, 4 holes/group.Ribavirin 6 administration groups each with extract are separately added into different dilution medicinal liquid, cell controls group and Virus control group adds maintaining liquid, 100 μ l/ holes.Culture plate puts 35 DEG C, 5%co2Cultivate in incubator, under daily inverted microscope Observation of cell pathological changes situation, records experimental result, calculates by reed-muench method when virus control group cytopathy reaches 4 grades Half-inhibition concentration ic50, select index si=tc50/ic50, experiment is repeated 3 times.As a result, the present embodiment extract sample is to first The ic of type influenza virus50Average out to 74.6 μ g/ml, si are 16.8, the ic to Influenza B viruss50Average out to 99.6 μ g/ml, Si is 12.7.It is shown in Table 2.
The impact to viral pathogenesis poison cytopathogenic effect for the table 2 the present embodiment extract
Above-mentioned test result indicate that: extract of the present invention has anti-Influenza B viruss effect in vitro.
Embodiment 6
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
30 parts of Fructus Momordicae charantiae, 30 parts of Herba Agrimoniae, 20 parts of Radix Ophiopogonis, 13 parts of bamboo vinegar liquid.
The method making described Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, the method comprising the steps of:
1) weigh raw material components, successively raw material is pulverized respectively with Chinese medicine grinder, each grinding time 8 minutes, Pulverized with super micron mill again after crossing 100 mesh sieves, standby；
2) by described step 1) pulverize after the organic solvent adding its 10 times of weight Radix Ophiopogonis, carry out ultrasonic-microwave cooperating Extract, filter to get filtrate, remove filtering residue concentrating under reduced pressure, drying, dried object is pulverized, obtains material 1；
3) by described step 1) pulverize after Herba Agrimoniae, with the pure water heating and refluxing extraction 2 times of 7 times amount, merge extraction Liquid, by gained lixiviating solution concentrating under reduced pressure, dry, pulverize, obtains material 2；
4) by described step 1) pulverize after Fructus Momordicae charantiae add distilled water, with cellulose treatment, filter and retain filtering residue, then to Wherein add distilled water, microwave treatment, then heating in water bath, filter to get filtrate, concentrating under reduced pressure, dry, pulverize, obtain material 3；
5) material 1, material 2, material 3 are mixed, smash, add the pure water of mixture identical weight, put into and divide at a high speed Scattered mulser is processed, and obtains high speed dispersion and emulsion thing；
6) pressurised extraction: by step 5) process after emulsion, put in pressure pan, add emulsion identical weight pure Water purification, pressurization and then recovery normal pressure, filter, obtain extracting solution, and be condensed into extractum, and concentrating under reduced pressure, drying obtain extract；
7) by bamboo vinegar liquid with combination of ultrasound electric field treatment, and it is condensed into extractum, concentrating under reduced pressure, drying, 55 DEG C of baking temperature, To moisture below 6%, pulverize, cross 80 mesh sieves, obtain bamboo vinegar extract；
8) by described step 6) extract that obtains, described step 7) mixing of bamboo vinegar extract；
Complete the making of extract.
Described step 2) organic solvent is the ethanol that volumetric concentration is 75%, the condition that ultrasonic-microwave cooperating extracts is: work( Rate 50w, frequency 20khhz, carry out ultrasonic-microwave cooperating and extract 800s, described alkali liquor is sodium hydrate aqueous solution.
Described step 2) material 1 makes and also includes: carries out the filtering residue after ultrasonic-microwave cooperating extracts and adds its 40 times of weight Sodium hydrate aqueous solution, adjust ph be 8, temperature be 85 DEG C, carry out extract 25min, filter resulting solution, with ultrasonic-microwave The collaborative filtrate mixing extracted, concentrating under reduced pressure, drying, dried object is pulverized, is obtained material 1.
Methods described also includes raw material Herba Agrimoniae and carries out efficacy of a drug fermentation pretreatment before pulverizing, and its processing method is: by celestial being Crane grass freezing processing, then steam treatment, be dried formed the efficacy of a drug fermentation Herba Agrimoniae carry out described step 1 again) pulverization process.
- 20 DEG C of described freezing processing temperature, freezing processing 6h, described vapor (steam) temperature is 70 DEG C, and steam pressure is 0.2mpa, Continue 32 minutes.
Described step 6) it is forced into 3.8kg/cm2, and keep 12min, in 2s, then recover normal pressure.
Described step 7) combination of ultrasound electric field treatment method is: first with frequency 55khz, supersound process 15min, then use intensity The Electric Pulse Treatment 8min of 8kv/cm.
The present embodiment extract is for hypoglycemic clinical observation result:
1.1 physical data: select the People's Hospital of city outpatient service type 2 diabetes mellitus patient 60, be randomly divided into two groups.Treatment group 30 Example, man 16, female 14,30～70 years old age, average (50 ± 1.5) year, the course of disease 6 months～15 years, average (8.0 ± 1.2) Year；Matched group 30；Man 18；Female 12,29～70 years old age.Two groups of physical data comparing differences not statistically significant (p > 0.05), there is comparability.
1.2 case selections: with reference to World Health Organization's (who) diabetes diagnosis in 1999 and parting standard: diabetes Shape+random time plasma glucose levels >=11.1mmol/l (200mg/dl).Patient's random packet of above-mentioned standard will be met Carry out observational study, the patient partly taking traditional Chinese medicine stops former Chinese medicine and includes observation after 2 weeks, the patient not taken traditional Chinese medicine is directly brought into sight Examine.Two groups of patients all give Diabetes Foundation to treat, and including diet control, exercise therapy and orally-taken blood sugar reducing Western medicine, blood sugar lowering Western medicine is adopted With metformin, drug usage and dose adjustment are carried out according to blood glucose situation.
1.3 Therapeutic Method: treatment group takes extract of the present invention on the basis of taking metformin.Take in four times daily With 5 grams every time.Matched group routine takes metformin.Two groups are consistent in diet control, exercise therapy, two groups of courses for the treatment of It is 2 months.
1.4 observation index: measure fasting glucose (fpg), post-prandial glycemia (2hbg), glycolated hemoglobin before and after treatment respectively (hba1c), fasting insulin (fins), blood glucose determination of glucose oxidase, insulin is measured with putting method of exempting from, and blood fat is with entirely Automatic biochemistry analyzer measures, and homa-ir adopts haffner etc. to calculate the formula of ir, i.e. homa-ir=in homa model Fpg × fins/22.5 (fpg unit is mmol/l, and fins unit is mu/l).
1.5 statistical methods: statistical procedures are carried out using spss 14.0 statistical analysis software.Measurement data adopts t to examine Test, enumeration data chi-square criterion.
2 results: before and after two groups of treatments, the change of fpg, 2hbg, hbaic, fins and homa-ir refers to table 1.By in table 3 I Can see, treatment the horizontal comparing difference of front two groups of patients fpg, 2hbg, hbalc, fins, homa-ir not statistically significant (p>0.05)；Before two groups of fpg, 2hbg, hbalc, fins, homa-ir levels and treatment after treatment, comparing difference has statistics to anticipate Adopted (p < 0.05 or p < 0.01), treatment group's patient's These parameters have significant difference (p < 0.01) with comparing before treatment.Treatment group All diabetes glucose can be reduced with matched group, but blood glucose be improved with treatment group and becomes apparent from.
The change of fpg, 2hbg, hbalc, fins and homa-ir before and after 3 liang of group patient's treatments of table
Note: indices compared with pre-treatment: * p < 0.05, * * p < 0.01；After treatment, two groups of fins and homa-ir compare ^ P < 0.05, ^^p < 0.01.
Embodiment 7
A kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material components by weight:
23 parts of Fructus Momordicae charantiae, 25 parts of Herba Agrimoniae, 16 parts of Radix Ophiopogonis, 11 parts of bamboo vinegar liquid.
The method making described Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, the method comprising the steps of:
1) weigh raw material components, successively raw material is pulverized respectively with Chinese medicine grinder, each grinding time 2-10 divides Clock, is pulverized with super micron mill after crossing 100 mesh sieves again, standby；
2) by described step 1) pulverize after the organic solvent adding its 8～15 times of weight Radix Ophiopogonis, carry out ultrasonic-microwave association With extracting, filter to get filtrate, remove filtering residue concentrating under reduced pressure, drying, dried object is pulverized, obtains material 1；
3) by described step 1) pulverize after Herba Agrimoniae, with pure water heating and refluxing extraction 2-3 time of 5-10 times amount, merging Lixiviating solution, by gained lixiviating solution concentrating under reduced pressure, dry, pulverize, obtains material 2；
4) by described step 1) pulverize after Fructus Momordicae charantiae add distilled water, with cellulose treatment, filter and retain filtering residue, then to Wherein add distilled water, microwave treatment, then heating in water bath, filter to get filtrate, concentrating under reduced pressure, dry, pulverize, obtain material 3；
5) material 1, material 2, material 3 are mixed in proportion, smash, add the pure water of identical weight, put into and divide at a high speed Scattered mulser is processed, and obtains high speed dispersion and emulsion thing；
6) pressurised extraction: by step 5) process after emulsion, put in pressure pan, add identical weight pure water, Pressurization and then recovery normal pressure, filter, obtain extracting solution, and be condensed into extractum, and concentrating under reduced pressure, drying obtain extract；
7) by bamboo vinegar liquid with combination of ultrasound electric field treatment, and it is condensed into extractum, concentrating under reduced pressure, drying, baking temperature 50-60 DEG C, to moisture below 6%, pulverize, cross 80～90 mesh sieves, obtain bamboo vinegar extract；
8) by described step 6) extract that obtains, described step 7) mixing of bamboo vinegar extract；
Complete the making of extract.
Described step 2) organic solvent is the ethanol that volumetric concentration is 65%～85%, the bar that ultrasonic-microwave cooperating extracts Part is: power 30～70w, frequency 12～30khhz, carries out ultrasonic-microwave cooperating and extracts 500～1200s, described alkali liquor is Sodium hydrate aqueous solution.
Described step 2) material 1 makes and also includes: carries out the filtering residue after ultrasonic-microwave cooperating extracts and adds its 30～50 weight The sodium hydrate aqueous solution of amount times, adjusting ph is 7～9, and temperature is 70～95 DEG C, carries out extracting 15～35min, filters gained molten Liquid, the filtrate extracted with ultrasonic-microwave cooperating is mixed, and dried object is pulverized, obtained material 1 by concentrating under reduced pressure, drying.
Methods described also includes raw material Herba Agrimoniae and carries out efficacy of a drug fermentation pretreatment before pulverizing, and its processing method is: by celestial being Crane grass freezing processing, then steam treatment, be dried formed the efficacy of a drug fermentation Herba Agrimoniae carry out described step 1 again) pulverization process.
- 25～-10 DEG C of described freezing processing temperature, freezing processing 3-7h, described vapor (steam) temperature is 60-75 DEG C, vapour pressure Power is 0.1-0.25mpa, continues 30～35 minutes.
Described step 6) it is forced into 3.5～4kg/cm2, and keep 10-15min, in 1-3s, then recover normal pressure.
Described step 7) combination of ultrasound electric field treatment method is: first with frequency 40～60khz, supersound process 5～20min, Use the Electric Pulse Treatment 5～10min of intensity 5～10kv/cm again.
Described concentrating under reduced pressure and baking temperature are 50-60 DEG C, are dried to moisture below 6%.
In vitro Bactericidal Experiments
Trial drug: the present embodiment extract.
Method: Chinese medicine of the present invention is measured to escherichia coli, staphylococcus aureuses, green using micro continuous doubling dilution Pus pseudomonass, staphylococcus epidermidiss, proteus vulgaris, the mark bacterial strain of streptococcus faecalis and escherichia coli, golden yellow Fructus Vitis viniferae Coccus, Pseudomonas aeruginosas, staphylococcus epidermidiss, proteus vulgaris, the minimal inhibitory concentration of streptococcus faecalis clinical separation strain (mic), the minimal bactericidal concentration (mbc) to above-mentioned antibacterial for the extract in the present embodiment is measured using flat board infection protocol.Will be big Enterobacteria, staphylococcus aureuses, Pseudomonas aeruginosas, staphylococcus epidermidiss, proteus vulgaris, it is inoculated in mh meat soup, put 37 DEG C of culture 24h of standard incubator；Streptococcus faecalis are inoculated in tpy meat soup (anaerobe nutrient broth), put 37 DEG C of anaerobic culture box Culture 48h.
The least concentration that bacterial growth in culture medium can be suppressed is minimal inhibitory concentration (minimal inhibitory Concentration, mic).During killing antibacterial for evaluation criteria, total viable count is made to reduce 99% or more than 99.5%, referred to as Minimal bactericidal concentration (minimal bactericidal concentration, mbc).A collection of experiment in can suppress 50% or Mic needed for 90% tested bacterium, is referred to as mic50And mic90.The bacteriostasis of antimicrobial drug are relative with bactericidal action, and some resist Bacterium medicine is in bacteriostasis in low concentration, and high concentration is in bactericidal action.
Mic and mbc to reference culture for table 4 pharmaceutical composition of the present invention
From upper table 4, the present embodiment extract is to the selected escherichia coli of test, staphylococcus aureuses, green pus Pseudomonass, staphylococcus epidermidiss, proteus vulgaris, streptococcus faecalis are clinically separated Reference Strains and all have and significantly suppress and go out Live and act on, its mbc is 2～4 times of mic.
Table 5 is the present embodiment extract mic, mic to 265 plants of Clinical isolation50、mic99With mbc (mg compositionss/ ml)
Mic and mbc to reference culture for table 5 pharmaceutical composition of the present invention
Bacterial strain Strain number mic mic50 mic99 mbc
Staphylococcus aureuses 100 0.40～1.47 1.032 1.31 0.75～6.31
Escherichia coli 110 6.39～48 17.861 22.146 30～800
Staphylococcus epidermidiss 30 0.35～3.12 0.712 1.4808 1.58～6.36
Proteus vulgaris 25 11.4～49 25.454 33.0531 35～88
From upper table 5, the present embodiment extract 265 plant Clinical isolation selected to test all have certain suppression System and deactivation, its mbc is 2～4 times of mic.
When removing bamboo vinegar liquid or Rhizoma Curcumae Longae in the present embodiment extract, it is compared with the present embodiment fungistatic effect, it is right to measure Inhibitory action mic (mg compositionss/ml) of bacterial strain.Comparing result is shown in Table 6.
Specifically comparative example is,
Comparative example 1: a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following raw material by weight Component: 23 parts of Fructus Momordicae charantiae, 25 parts of Herba Agrimoniae, 16 parts of Radix Ophiopogonis.
Comparative example 2: a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following parts by weight of component: Fructus Momordicae charantiae 23 parts, 16 parts of Radix Ophiopogonis, 11 parts of bamboo vinegar liquid.
Comparative example 3: a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract, described extract includes following parts by weight of component: Fructus Momordicae charantiae 23 parts, 25 parts of Herba Agrimoniae.
Contrast on effect to the mic of reference culture in table 5 instant component
As shown in Table 5, for having fungistatic effect, both are used cooperatively with other components for the Radix Ophiopogonis in prescription, bamboo vinegar liquid, Effect is optimum.
Choosing has the crowd totally 200 of various oral cavities malaise symptoms, and the wherein personnel based on oral ulcer symptom have 110, The personnel of laryngopharynx swelling and pain symptom have 90, take the Chinese medicinal tablet of the embodiment of the present invention 10 to above-noted persons, and instructions of taking is: every It 3 times, each 5mg.Continuously take observed result after the week, in 110 of oral ulcer symptom, have 100 feels pain Substantially mitigate, ulcer surface is no longer rubescent, and remaining personnel's feels pain slightly mitigates, and effect is less obvious, effective percentage is 90.1%. In 90 of laryngopharynx swelling and pain, 75 pain are had substantially to mitigate, remaining personnel's sensory effect is inconspicuous, effective percentage is 83%.
The above embodiments are only the preferred technical solution of the present invention, and are not construed as the restriction for the present invention, this Shen Please in embodiment and the feature in embodiment in the case of not conflicting, can mutual combination in any.The protection model of the present invention Enclose the technical scheme that should record with claim, the equivalent side of technical characteristic in the technical scheme recorded including claim Case is protection domain.I.e. equivalent within this range is improved, also within protection scope of the present invention.

Claims (10)

1. a kind of Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract are it is characterised in that described extract includes following by weight former Material component:
20 ~ 30 parts of Fructus Momordicae charantiae, 15 ~ 30 parts of Herba Agrimoniae, 10 ~ 20 parts of Radix Ophiopogonis.
2. according to claim 1 Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract it is characterised in that described extract also include following Parts by weight of component:
8 ~ 13 parts of bamboo vinegar liquid.
3. make claim 2 described in Fructus Momordicae charantiae, Herba Agrimoniae, Radix Ophiopogonis extract method it is characterised in that methods described include with Lower step:
1) weigh raw material components, successively raw material is pulverized respectively with Chinese medicine grinder, each grinding time 2-10 minute, mistake Pulverized with super micron mill again after 100 mesh sieves, standby；
2) by the organic solvent adding its 8～15 times of weight Radix Ophiopogonis after the pulverizing of described step 1), carry out ultrasonic-microwave cooperating and carry Take, filter to get filtrate, remove filtering residue concentrating under reduced pressure, drying, dried object is pulverized, obtains material 1；
3) by the Herba Agrimoniae after the pulverizing of described step 1), with water heating and refluxing extraction 2-3 time of 5-10 times of weight, merge and extract Liquid, by gained lixiviating solution concentrating under reduced pressure, dry, pulverize, obtains material 2；
4) Fructus Momordicae charantiae after the pulverizing of described step 1) is added distilled water, with cellulose treatment, filter and retain filtering residue, more thereto Add distilled water, microwave treatment, then heating in water bath, filter to get filtrate, concentrating under reduced pressure, dry, pulverize, obtain material 3；
5) material 1, material 2, material 3 are mixed in proportion, smash, add the water of mixture identical weight, put into dispersion at a high speed Mulser is processed, and obtains high speed dispersion and emulsion thing；
6) pressurised extraction: the emulsion after step 5) is processed, put in pressure pan, add the water of emulsion identical weight, plus Pressure and then recovery normal pressure, filter, obtain extracting solution, and be condensed into extractum, and concentrating under reduced pressure, drying obtain extract；
7) by bamboo vinegar liquid with combination of ultrasound electric field treatment, and it is condensed into extractum, concentrating under reduced pressure, drying, 50-60 DEG C of baking temperature, To moisture below 6%, pulverize, cross 80 ~ 90 mesh sieves, obtain bamboo vinegar extract；
8) extract that described step 6) obtained, the mixing of described step 7) bamboo vinegar extract；
Complete the making of extract.
4. according to claim 3 method it is characterised in that: described step 2) organic solvent be volumetric concentration is 65%～85% Ethanol, ultrasonic-microwave cooperating extract condition be: power 30～70w, frequency 12～30 khhz, carry out ultrasonic-microwave association With extraction 500～1200 s.
5. according to claim 3 method it is characterised in that: described step 2) material 1 makes and also includes: carry out ultrasonic-micro- The filtering residue that ripple is worked in coordination with after extraction adds the sodium hydrate aqueous solution of its 30～50 times of weight, and adjusting ph is 7～9, and temperature is 70～ 95 DEG C, carry out extracting 15～35min, filter resulting solution, the filtrate extracted with ultrasonic-microwave cooperating mix, concentrating under reduced pressure, do Dry, dried object is pulverized, is obtained material 1.
6. method according to claim 3 is it is characterised in that methods described also includes raw material Herba Agrimoniae carries out before pulverizing Efficacy of a drug fermentation pretreatment, its processing method is: by Herba Agrimoniae freezing processing, then steam treatment, dry formation efficacy of a drug fermentation red crowned crane Grass carries out described step 1) pulverization process again.
7. method according to claim 6 it is characterised in that: -25～-10 DEG C of described freezing processing temperature, freezing processing 3-7 h, described vapor (steam) temperature is 60-75 DEG C, and steam pressure is 0.1-0.25mpa, continues 30 ~ 35 minutes.
8. method according to claim 3 it is characterised in that: described step 6) is forced into 3.5 ~ 4 kg/cm2, and keep 10-15min, then recovers normal pressure in 1-3s.
9. method according to claim 3 it is characterised in that: described step 7) combination of ultrasound electric field treatment method is: first With frequency 40 ~ 60khz, supersound process 5 ~ 20min, then Electric Pulse Treatment 5 ~ 10 min with intensity 5 ~ 10kv/cm.
10. the extract described in claim 1 or 2 or the method described in claim 3-9 any one treatment influenza virus, Application in blood sugar lowering.
CN201610879054.9A 2016-10-08 2016-10-08 Bitter gourd, agrimonia pilosa ledeb and radix ophiopogonis extract and preparation method and application thereof Pending CN106361967A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110278949A (en) * 2019-07-19 2019-09-27 广东龙能富碳农业科技有限公司 A kind of sterilization insect resistant agent and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101293065A (en) * 2008-03-04 2008-10-29 何明华 Biological proprietary Chinese medicine for preventing and treating diabetes
CN103446503A (en) * 2013-07-26 2013-12-18 杨德俊 Traditional Chinese medicine composition for treating type II diabetes mellitus
CN104645013A (en) * 2013-11-25 2015-05-27 陕西康乐中医药养生研究院 Traditional Chinese medicine decoction for treating diabetes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101293065A (en) * 2008-03-04 2008-10-29 何明华 Biological proprietary Chinese medicine for preventing and treating diabetes
CN103446503A (en) * 2013-07-26 2013-12-18 杨德俊 Traditional Chinese medicine composition for treating type II diabetes mellitus
CN104645013A (en) * 2013-11-25 2015-05-27 陕西康乐中医药养生研究院 Traditional Chinese medicine decoction for treating diabetes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110278949A (en) * 2019-07-19 2019-09-27 广东龙能富碳农业科技有限公司 A kind of sterilization insect resistant agent and preparation method thereof

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