CN106344456A - Application of melia azedarach leaf extract in whitening product - Google Patents
Application of melia azedarach leaf extract in whitening product Download PDFInfo
- Publication number
- CN106344456A CN106344456A CN201610882781.0A CN201610882781A CN106344456A CN 106344456 A CN106344456 A CN 106344456A CN 201610882781 A CN201610882781 A CN 201610882781A CN 106344456 A CN106344456 A CN 106344456A
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- Prior art keywords
- leaf extract
- whitening
- chinaberry leaf
- application
- whitening product
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Life Sciences & Earth Sciences (AREA)
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- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention provides application of melia azedarach leaf extract in a whitening product. A preparation method of the melia azedarach leaf extract comprises the following steps: smashing melia azedarach leaves; putting the melia azedarach leaves in a supercritical CO2 extracting device, and extracting for 2 hours, thus obtaining medicine residues; adding 75 percent of alcohol in the medicine residues; leaching twice under 60 DEG C; merging leaching solutions; carrying out vacuum concentration and freeze drying, thus obtaining the melia azedarach leaf extract. The melia azedarach leaf extract provided by the invention contains whitening active ingredients, is capable of obviously inhibiting the activity of tyrosinase and hindering the formation of melanin, is safe in source, and has very good whitening development application value.
Description
Technical field
The present invention relates to natural cosmetics technical field and in particular to a kind of chinaberry leaf extract in whitening product should
With.
Background technology
In recent years, due to the increase of life and work pressure and the aggravation of environmental pollution, the skin problem faced by people is also got over
Come more, the mottle that caused by a variety of causes, pigmentation etc. become common problem, based on whitening has become with oxidation resistant product
Family status kind.Increasingly mature with people's consumption idea, consumer is not only only that its effect to the focal point of whitening product
Property also more focuses on its safety simultaneously.
The reason skin splash, pigmentation is caused to have multiple, such as the ring such as inherited genetic factorss and ultraviolet, waste gas and active oxygen
Border factor.Current result of study thinks that melanin is mainly produced by the melanocyte of human body skin stratum basale, it
Content and distribution determine the color of skin.Modern study shows, tryrosinase is the biosynthetic key enzyme of dermal melanin,
Tyrosine is changed into DOPA, DOPA quinone by it, oxidized and be polymerized the melanin generating macromole.Therefore, tryrosinase is not only
Determine the speed of B16 cell, and be the ripe characteristic mark of Differentiation of Melanocytes, its activity decides melanin
The quantity being formed.Therefore, the activity of suppression tryrosinase can block melanic biosynthesis reaction chain, reduces melanic
Generate and then realize the effect of whitening.Conventional whitening additive mainly has compound containing mercury and hydroquinone, kojic acid etc..But due to containing
The toxicity of mercury compound and the sensitization of hydroquinone, kojic acid is under a cloud carcinogenesis, so these compounds are rarely employed.
And the tyrosinase activity inhibitor of plant origin becomes due to catering to the demand for security of people in Recent study application
One of focus.Meanwhile, many plant extracts have its unique effect, so the suppression ratio of tryrosinase is better than traditional whitening
Agent.
Chinaberry, chinaberry of again grieving, is winter deciduous tree, and it has growth rapidly, and broad-spectrum feature causes many
The interest of research worker.Chinaberry leaf has functions that regulating QI to expel dampness, removing toxic substances parasite killing, and chinaberry leaf extract is as botanical pesticide
And be widely used at aspects such as integrated pest managements, and whitening product is used for for active component in chinaberry leaf
Relevant report is less.
Content of the invention
For the deficiencies in the prior art, the invention provides a kind of application in whitening product for chinaberry leaf extract, tool
There is suppression tyrosinase activity, reduce the effect that melanin produces.
For realizing object above, the present invention is achieved by the following technical programs:
Application in whitening product for the chinaberry leaf extract.
The preparation method of described chinaberry leaf extract is as follows: chinaberry leaf is pulverized, is placed in supercritical co2In extraction equipment,
Extraction 2h, obtains medicinal residues, adds the volume mass of 75% ethanol, ethanol and medicinal residues ratio for 8ml:1g, soak at 60 DEG C in medicinal residues
Carry 2 times, each 1h, merge lixiviating solution, concentrating under reduced pressure, lyophilization, obtain final product.
Preferably, described extraction kettle temperature is 50 DEG C, and pressure is 40mpa, and the first separating still temperature is 40 DEG C, and pressure is
5mpa, the second separating still temperature is 35 DEG C, and pressure is 4mpa, co2Flow is 32l/h, extracts 2h.
Preferably, the condition of described concentrating under reduced pressure is 70 DEG C, 0.2bar.
The form of described whitening product includes facial film, moisture retention water, emulsion, facial cream, bath foam, foundation cream.
Preferably, oil components, inorganic filler, surfactant, wetting agent, thickening are also included in described whitening product
In agent, lower alcohol, polyvalent chelate compound, organic amine, ph regulator, preservative, other whitening agent, antioxidant, spice and water
At least one.
The invention has the advantages that: the chinaberry leaf extract that the present invention provides contains whitening active ingredients, can substantially suppress
The activity of tryrosinase, hinders melanic formation, and safe source, has good whitening development and application values.
Specific embodiment
Purpose, technical scheme and advantage for making the embodiment of the present invention are clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the present invention is clearly and completely described it is clear that described embodiment is the present invention one
Divide embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making
The every other embodiment being obtained under the premise of creative work, broadly falls into the scope of protection of the invention.
Embodiment 1:
The preparation method of chinaberry leaf extract is as follows: chinaberry leaf is pulverized, is placed in supercritical co2In extraction equipment, control
Extraction kettle temperature is 50 DEG C, and pressure is 40mpa, and the first separating still temperature is 40 DEG C, and pressure is 5mpa, and the second separating still temperature is
35 DEG C, pressure is 4mpa, co2Flow is 32l/h, extracts 2h, obtains medicinal residues, add 75% ethanol, ethanol and medicinal residues in medicinal residues
Volume mass ratio for 8ml:1g, extract 2 times at 60 DEG C, each 1h, merge lixiviating solution, at 70 DEG C, under conditions of 0.2bar
Concentrating under reduced pressure, lyophilization, obtain final product.
Embodiment 2:
The suppression tyrosinase activity experiment of chinaberry leaf extract:
Take 5 test tubes, in wherein No. 1 and No. 2 test tubes, add the 0.05wt% chinaberry leaf extract solution of 1ml, No. 3 and 4
Number test tube is separately added into the phosphate buffer (ph=6.8) of 1ml, adds the 0.05wt% arbutin of 1ml molten in No. 5 test tubes
Liquid.Add the tyrosinase solution (enzyme-activity unit 125u/ml) of 0.5ml in 1,3 and No. 5 test tubes, add in No. 2 and No. 4 test tubes
Enter the phosphate buffer (ph=6.8) of 0.5ml, 5 test tube vibrations are shaken up, 35 DEG C of water-bath 10min, be added thereto to 2ml
0.05wt% tyrosine solution, react 10min, 475nm at mensure each test tube absorbance t, be respectively labeled as t1, t2,
T3, t4, t5, and calculate suppression ratio.
Suppression ratio computing formula is: i (%)=[1- (t1-t2)/(t3-t4)] × 100%
Be computed, 0.05wt% chinaberry leaf extract solution to the suppression ratio of tryrosinase up to 81.1%, and conduct
The suppression ratio of the arbutin of comparison is only 48.1%.As can be seen here, chinaberry leaf extract has preferable suppression for tryrosinase
Activity.
Embodiment 3:
Chinaberry leaf extract is tested to melanin inhibitory action:
Chinaberry leaf extract is measured to melanin inhibitory action using modification methods such as hosoi.(the purchase of b16 melanocyte
From Shanghai Yan Sheng Industrial Co., Ltd.) cultivated in 96 orifice plates with 1 × 105 density, change liquid after 24h, add 0.05wt% chinaberry
Leaf extract solution, after 72h, washes twice through phosphate buffer (ph=6.8), and sample is dried through air, is dissolved in 200 μ
L1mol/l naoh (containing 1%dmso), heated 80 DEG C cool down to 1h, select 475nm wavelength on enzyme-linked immunosorbent assay instrument
Read absorbance, calculate suppression ratio；The absorbance of 0.05wt% arbutin is measured using same procedure and calculates suppression ratio.
Be computed, 0.05wt% chinaberry leaf extract solution to melanic suppression ratio up to 69.8%, and as right
According to the suppression ratio of arbutin be only 48.1%.As can be seen here, chinaberry leaf extract is inhibited for melanin, can
Skin whitening.
Embodiment 4:
Whitening cream containing chinaberry leaf extract, each constituent mass percent is as follows: chinaberry leaf extract 1%, poly- two
Methylsiloxane 2%, isopropyl myristate 10%, 2-Methylpentadecane 4%, glycerol 5%, propylene glycol 3%, glyceryl stearate
1%th, Carbomer 0.5%, methyl parahydroxybenzoate 0.1%, vitamin e 1%, Parleam 3%, triethanolamine are fitted
Amount, appropriate essence, balance deionized water.
The preparation technology of whitening cream is processed according to the preparation technology of conventional creams.
By the present embodiment be obtained whitening cream be used for 18-40 year, face have different degrees of pigmentation, dull-looking skin,
The problems such as be dried matt, and exclude with crowds such as serious systemic disease, immunodeficiency, autoimmune diseases.Experimenter is every
After cleaning skin of face before sleeping day, coat whitening cream manufactured in the present embodiment, continuously use one month, have 70% experimenter
Skin have substantially dark yellow disappear, skin moisten, the skin having 20% experimenter makes moderate progress, skin relatively before pale, and
In tested 100 people, the symptoms such as skin allergy do not occur.This result shows, the chinaberry leaf extract of the present invention being capable of limited improvement face
Portion's colour of skin, plays whitening effect.
Embodiment 5:
Whitening cleansing facial milk containing containing chinaberry leaf extract, each constituent mass percent is as follows: chinaberry leaf extract 3%, alkane
Alkyl polyglucosides 10%, polyoxyethylenated alcohol sodium sulfate 5%, fatty acid distribution of coconut oil monoethanolamine 4%, cocamidopropyl propyl amide Radix Betae
Alkali 5%, glycerol 10%, squalane 3%, Methylisothiazolinone 0.05%, lanoline 1%, stearic acid 2%, deionized water
Surplus.
The preparation technology of whitening cleansing facial milk containing is processed according to the preparation technology of conventional cleansing milk.
In sum, the chinaberry leaf extract that the present invention provides contains whitening active ingredients, can substantially suppress tyrosine
The activity of enzyme, hinders melanic formation, has good whitening development and application values.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation are made a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " inclusion ", "comprising" or its any other variant are intended to
Comprising of nonexcludability, wants so that including a series of process of key elements, method, article or equipment and not only including those
Element, but also include other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element that limited by sentence "including a ..." it is not excluded that
Also there is other identical element including in the process of described key element, method, article or equipment.
Above example only in order to technical scheme to be described, is not intended to limit；Although with reference to the foregoing embodiments
The present invention has been described in detail, it will be understood by those within the art that: it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or carries out equivalent to wherein some technical characteristics；And these modification or
Replace, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (6)
1. application in whitening product for the chinaberry leaf extract.
2. application in whitening product for the chinaberry leaf extract as claimed in claim 1 is it is characterised in that described chinaberry leaf carries
Take the preparation method of thing as follows: chinaberry leaf to be pulverized, is placed in supercritical co2In extraction equipment, extract 2h, obtain medicinal residues, to medicinal residues
Middle addition 75% ethanol, the volume mass of ethanol and medicinal residues ratio for 8ml:1g, extracts 2 times at 60 DEG C, each 1h, merges extraction
Liquid, concentrating under reduced pressure, lyophilization, obtain final product.
3. application in whitening product for the chinaberry leaf extract as claimed in claim 2 is it is characterised in that described extraction kettle temperature
Spend for 50 DEG C, pressure is 40mpa, the first separating still temperature is 40 DEG C, pressure is 5mpa, the second separating still temperature is 35 DEG C, pressure
Power is 4mpa, co2Flow is 32l/h, extracts 2h.
4. application in whitening product for the chinaberry leaf extract as claimed in claim 2 is it is characterised in that described concentrating under reduced pressure
Condition be 70 DEG C, 0.2bar.
5. application in whitening product for the described chinaberry leaf extract as arbitrary in claim 1-4 is it is characterised in that described U.S.
The form of white product includes facial film, moisture retention water, emulsion, facial cream, bath foam, foundation cream.
6. application in whitening product for the chinaberry leaf extract as claimed in claim 1 is it is characterised in that described whitening product
In also include oil components, inorganic filler, surfactant, wetting agent, viscosifier, lower alcohol, polyvalent chelate compound, organic
At least one in amine, ph regulator, preservative, other whitening agent, antioxidant, spice and water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610882781.0A CN106344456A (en) | 2016-10-08 | 2016-10-08 | Application of melia azedarach leaf extract in whitening product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610882781.0A CN106344456A (en) | 2016-10-08 | 2016-10-08 | Application of melia azedarach leaf extract in whitening product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106344456A true CN106344456A (en) | 2017-01-25 |
Family
ID=57865742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610882781.0A Pending CN106344456A (en) | 2016-10-08 | 2016-10-08 | Application of melia azedarach leaf extract in whitening product |
Country Status (1)
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| CN (1) | CN106344456A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108685795A (en) * | 2018-08-02 | 2018-10-23 | 芜湖扬展新材料科技服务有限公司 | A kind of preparation method of the Whitening, spot lotion of the leaf extract containing chinaberry |
| CN110547985A (en) * | 2019-08-09 | 2019-12-10 | 东晟源研究院(广州)有限公司 | Formula process and preparation method of multi-dimensional whitening with yellow removal function |
| CN110711161A (en) * | 2019-11-03 | 2020-01-21 | 广州悦荟化妆品有限公司 | Leave-on allergy-relieving repair essence mask |
| EP3741379A4 (en) * | 2019-02-20 | 2022-01-26 | Honest Co.,Ltd. | Cosmetic composition comprising azadirachta indica leaf extract |
| US11291647B2 (en) * | 2018-08-30 | 2022-04-05 | Nisarga Biotech Private Limited | Process for preparation of CO2 extract of Azadirachta indica and herbal compositions thereof for treatment of cancers |
| CN114306148A (en) * | 2021-08-26 | 2022-04-12 | 欧诗漫生物股份有限公司 | Whitening composition for Asian skin and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368046A (en) * | 2002-01-30 | 2002-09-11 | 云南中科生物产业有限公司 | Nim cosmetics series based on active component of nim for daily use and its preparing process |
| JP2006076909A (en) * | 2004-09-09 | 2006-03-23 | Ichimaru Pharcos Co Ltd | Method for producing neem extract and low irritating cosmetic composition containing the same |
-
2016
- 2016-10-08 CN CN201610882781.0A patent/CN106344456A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368046A (en) * | 2002-01-30 | 2002-09-11 | 云南中科生物产业有限公司 | Nim cosmetics series based on active component of nim for daily use and its preparing process |
| JP2006076909A (en) * | 2004-09-09 | 2006-03-23 | Ichimaru Pharcos Co Ltd | Method for producing neem extract and low irritating cosmetic composition containing the same |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108685795A (en) * | 2018-08-02 | 2018-10-23 | 芜湖扬展新材料科技服务有限公司 | A kind of preparation method of the Whitening, spot lotion of the leaf extract containing chinaberry |
| US11291647B2 (en) * | 2018-08-30 | 2022-04-05 | Nisarga Biotech Private Limited | Process for preparation of CO2 extract of Azadirachta indica and herbal compositions thereof for treatment of cancers |
| EP3741379A4 (en) * | 2019-02-20 | 2022-01-26 | Honest Co.,Ltd. | Cosmetic composition comprising azadirachta indica leaf extract |
| CN110547985A (en) * | 2019-08-09 | 2019-12-10 | 东晟源研究院(广州)有限公司 | Formula process and preparation method of multi-dimensional whitening with yellow removal function |
| CN110711161A (en) * | 2019-11-03 | 2020-01-21 | 广州悦荟化妆品有限公司 | Leave-on allergy-relieving repair essence mask |
| CN114306148A (en) * | 2021-08-26 | 2022-04-12 | 欧诗漫生物股份有限公司 | Whitening composition for Asian skin and preparation method and application thereof |
| CN114306148B (en) * | 2021-08-26 | 2023-12-05 | 欧诗漫生物股份有限公司 | Whitening composition for Asian skin and preparation method and application thereof |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170125 |
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