CN106333084A - Application method of phytase in feed - Google Patents
Application method of phytase in feed Download PDFInfo
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- CN106333084A CN106333084A CN201611054016.6A CN201611054016A CN106333084A CN 106333084 A CN106333084 A CN 106333084A CN 201611054016 A CN201611054016 A CN 201611054016A CN 106333084 A CN106333084 A CN 106333084A
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- phytase
- enzyme
- phosphorus
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Abstract
The invention discloses an application method of phytase in feed. The application method includes the steps that prepared liquid phytase is sprayed on bentonite in the mass ratio of 1:(8-15), enzyme-containing bentonite is prepared, the enzyme-containing bentonite and particle feed are evenly stirred during feeding, and the mass ratio of stirred materials is 1:(9,800-10,500); in the particle feed, the using amount of inorganic phosphorus accounts for 35% to 40% of the original using amount. According to the application method, the requirements of high-temperature prilling for the phytase are reduced, the special extracting process of enzymes is not required, collection of the enzymes at the normal temperature can also be guaranteed, the using ratio of phytate phosphorus in pig feed is increased by 20% to 40%, and the using ratio of phytate phosphorus in poultry feed is increased by 31% to 58%; mineral elements and nutrient substances combined with phytic acid are released, the production performance of poultry is improved, and the quality of egg poultry shells is improved; the discharging amount of phosphorus in animal faces is decreased, and environment pollution is reduced; insufficient phosphorus supplying is relieved; the addition amount of the phosphorus-containing feed is saved, and the feed cost is reduced.
Description
Technical field
The present invention relates to a kind of application process of phytase, application process in feed for more particularly, to a kind of phytase.
Background technology
In plant feed, the phosphorus of 60%-70% is presented in phytate phosphorus, nonruminant lack phytase thus difficult profit
Use phytic acid therein, major part excretes with excrement, cause the environmental pollution of the phosphorus of common people's concern.Meanwhile, it is full
The needs to phosphorus for the sufficient livestock and poultry, also need to add expensive inorganic phosphate in feed.But China is that a phosphorus source is in great shortage
Country, the yield of phosphorus less than 1/10th of demand, phosphorus source rapid rise of price, bring very big warp to Feed Manufacturing and raiser
Ji pressure.The production of Phos also brings serious environmental pollution simultaneously.In science and technology is constantly flourishing environmental pollution increasingly
In the case of serious, reduce the problem that Production of Livestock and Poultry is that Feedstuff Enterprises need research to the pollution of environment as far as possible.
Phytase, as a kind of green additive, at home and abroad all has larger development in recent years, and usage amount rises year by year,
Dose the digestibility that phytase not only can improve phytate phosphorus in animal and fowl fodder, mitigate the phosphorus pollution that aquaculture is brought, protection ring
Border, can also improve the production performance of the utilizing status raising livestock and poultry of protein, amino acid or polypeptide, energy, reduce feed
Cost, this feature of environmental protection micro-organism enzyme preparation will to improved soil, prevent water pollution and keep benign ecological cycles have
Active influence, immeasurable contribution is made to feed industry and aquaculture.
In the bacterial strain that native phytase produces, the content of phytase is too low it is difficult to a large amount of produce, and adds production cost mistake
High factor, limits phytase and uses in production practices.Existing phytase in use, through storing, will add
The processes such as work, granulation, can produce a certain degree of impact to phytase activity.Report at present and be separated to from mesophilic microorganism
Several macrotherm phytases, its optimum temperature, at 70-80 DEG C, has good temperature tolerance, but their enzyme activity at 37 DEG C are extremely low,
There is no use value in feed.The phytase of merchandized handling, has high enzyme activity at 37 DEG C at present, but it is not amenable to make again
High temperature during grain.Research institution and company enter from two thinkings of molecular modification screening natural new gene and existing gene both at home and abroad
Hand, but also do not obtain remarkable break-throughs.
Content of the invention
In order to solve the deficiencies in the prior art, it is an object of the invention to provide a kind of application in feed for phytase
Method, solves the impact to phytase for the high/low temperature, gives full play to its effect in feed.
For reaching above-mentioned purpose, the technology used in the present invention means are: a kind of application process in feed for phytase,
The liquid phytase of preparation is sprayed on bentonite according to mass ratio 1:8～15, prepares bentonite containing enzyme, will contain in feeding
Enzyme bentonite is fed with the uniform spice of pellet, and spice mass ratio is 1:9800～10500；Inorganic in described pellet
Phosphatic usage amount is the 35%～40% of former usage amount.
Further, the liquid phytase of described preparation refers to: aspergillus niger, saccharomycete, bacillus subtilis are put into training
In foster base, add methyl alcohol as derivant, both obtain after fermentation, fermentation time 5～7d, temperature sets 28～30 DEG C.
Further, described culture medium refers to: with following ratio, in 1000ml water, adds the Huang of 10ml content 1%
Soya-bean cake diffusion juice, 10g glucose, 3g peptone, 2.5g nacl, 2g caco3, 121 DEG C of moist heat sterilization 20min can inoculate.
Further, phytic acid enzyme stabilizers are also comprised in described bentonite containing enzyme, using dark colored plastic bottle packaging.
The invention has the beneficial effects as follows: ingenious technological design, decrease the requirement to phytase for the high temperature granulation, without enzyme
Special abstraction process, can guarantee that the preservation under enzyme normal temperature, in pig feed after adding phytase, phytic acid phosphorus utilization improves 20 again
～40%；In poultry feed, phytic acid phosphorus utilization improves 31～58%；Discharge the other mineral elements being combined with phytic acid and nutrients
Matter, improves the production performance of livestock and poultry, improves egg birds, beasts and eggs shell quality；Reduce the excretion of phosphorus in animal wastes, slow down phosphorus to environment
Pollution；Alleviate China's phosphorus source deficient, the under-supply situation of phosphorus；Save phosphorous feed, such as the interpolation of calcium monohydrogen phosphate, bone meal etc.
Amount, increases formula adjustment space, reduces feed cost.
Specific embodiment
With reference to embodiment, the present invention is further elaborated.
A kind of application process in feed for phytase, the liquid phytase of preparation is sprayed according to mass ratio 1:8～15
On bentonite, prepare bentonite containing enzyme, enzyme bentonite will be contained in feeding and feed with the uniform spice of pellet, spice quality
Than for 1:9800～10500；In described pellet, the usage amount of inorganic phosphate is the 35%～40% of former usage amount.
The liquid phytase of described preparation refers to: aspergillus niger, saccharomycete, bacillus subtilis is put in culture medium, adds
Plus methyl alcohol is as derivant, both obtain after fermentation, fermentation time 5～7d, temperature sets 28～30 DEG C.
Producing the bacterial classification used by enzyme preparation needs using less expensive and simple raw material, in the condition without derivant
Lower easily growth, and grow rapid, the enzyme of generation can be easily separated purifying and concentrates, enzymatic activity height, good stability, and activity is general will
Width, microbes producing cellulase should have stable physiological property, and is formed without malicious or to immunity metabolin.
Described culture medium refers to: with following ratio, in 1000ml water, the soyabean cake diffusion juice of addition 10ml content 1%,
10g glucose, 3g peptone, 2.5g nacl, 2g caco3, 121 DEG C of moist heat sterilization 20min can inoculate.Enzyme activity is all stablized
More than 10000～20000u/ gram, safety non-toxic, the phytic acid enzyme product produced is stablized more than 5000u/ gram.
Can also adopt: in 1000ml water, add 100g rice meal, 22g bean powder, 5g yeast, 1g kh2po4,
1gnacl, boils to pasty state, is cooled to room temperature, adds carbohydrase, adjusts ph value to after 7.5, adds 4gcaco3.The training of this ratio
Foster base, without derivant, but discovery after research, its effect and interpolation derivant, particularly methyl alcohol is as the effect of derivant
Fall far short, therefore, abandon this kind of culture medium.
In the selection of culture medium, the thinking of inventor is:
Because culture medium is different, the specific growth rate of microorganism and enzyme Forming ability are also different, thus provide suitable in the medium
When and abundant nutrients is thalli growth and a large amount of important prerequisite producing of enzyme.Raw materials used main inclusion in enzyme preparation production
Carbon source (including the monosaccharide and disaccharide that can utilize rapidly and the slow starch utilizing, cellulose polysaccharide etc.), nitrogen source (include beancake powder,
The organic nitrogen sources such as peanut dregses, fish meal, dried silkworm chrysalis meal, dusty yeast, corn steep liquor and ammonium salt, nitrate etc. are inorganic nitrogen-sourced), inorganic salts and
Inducer, solid fermentation will add a certain amount of carrier such as rice husk, maize peel toward contact.It is experimentally confirmed, carbon in culture medium
The ratio of source and nitrogen source is carbon-nitrogen ratio (generally being represented with c/n), directly affects the generation of growth, breeding and the enzyme of thalline.Work as c/n
When ratio is too small, that is, in culture medium, nitrogen source is excessive, causes growth of microorganism to cross Sheng, and carbon source deficiency easily causes thalline old and feeble and
Self-dissolving, causes nitrogen source to waste and production of enzyme declines；If c/n ratio is too high, that is, nitrogen source is not enough, and growth of microorganism is excessively slow, a side
Face easily causes miscellaneous bacteria to infect, and on the other hand due to not having the microorganism of q.s, will also result under carbon source waste and production of enzyme
Fall.Therefore, suitable c/n ratio should rightly be selected according to the characteristic of various microorganisms, be the important measures improving yield of enzyme.
Various inorganic salts with trace element as phosphorus, sulphur, magnesium, iron, potassium, receive, calcium, zinc, manganese etc. be microbial cell structure important become
Point or maintain enzymatic activity material, can promote growth of microorganism, growths, stimulate enzyme generation, indispensable in culture medium, raw material with
When having contained enough inorganic elements in water, it is not required to separately add.
The production of microbial enzyme, mainly adopts liquid deep layer fermenting and solid fermentation two ways at present.In this application
Using liquid deep layer fermenting, 1, the liquid suspension state that has the advantage that is the environment of Adaptable growth of many microorganisms；2nd, exist
In liquid environment, thalline, substrate, product (including heat) are easy to spread, and make fermentation in homogeneous or intend carrying out under processing condition, are easy to
Detection, control, easily expand the scale of production；3rd, liquid conveying convenient it is easy to mechanized operation；4th, product is easy to Hydrolysis kinetics.
Phytic acid enzyme stabilizers are also comprised, using dark colored plastic bottle packaging in described bentonite containing enzyme.
The extraction of enzyme: in fermentation substrate, enzyme is all existed jointly with other materials substantial amounts of, and the content of enzyme is than other
Material wants much less, and enzyme need to be separated from other materials substantial amounts of by therefore industrial enzymes.But in technical scheme
With enzyme need not pure enzyme, due to matrix and thalline used by fermentation in growth course produced amino acid, vitamin, nucleotides,
The compositions such as somatomedin are all favourable to growth of animals or poultry, and therefore, fodder enzyme need not refine.
The preservation of enzyme: enzyme is bioactivator, acid, alkali, organic solvent, heavy metal, surfactant, high temperature, ultraviolet
Some chemical factors such as line easily cause enzymatic structure to change, and cause the inactivation of enzyme, during preservation it should be noted that avoid these unfavorable because
Element.The long-term non-inactivation of enzyme to be kept, it is critical to control moisture and two conditions of temperature.Enzyme easy in inactivation when typically water content is high,
Water content more than 10%, equal easy in inactivation under room temperature and low temperature；When water content is down to 5%, more stable under room temperature or low temperature, temperature
Higher, more easy in inactivation；Exposure irradiation also causes the inactivation of some enzymes sometimes, and therefore enzyme should keep out of the direct sun.Some heavy metals
Ion (as copper, iron, lead, mercury, silver etc.) can resist the vigor of enzyme, or even enzyme can be made to inactivate, and should avoid connecing with these materials as far as possible
Touch.Phytase is sprayed on bentonite by the present invention, in long term storage, also can add phytic acid enzyme stabilizers, and using dark
Plastic bottle packing is it is ensured that it is active.
Compared with prior art, it is an advantage of the current invention that:
(1) in pig feed after adding phytase, phytic acid phosphorus utilization improves 20%～40%；In poultry feed, phytic acid phosphorus utilization carries
High by 31%～58%；Discharge the other mineral elements being combined with phytic acid and nutriment, improve the production performance of livestock and poultry, improve egg fowl
Quality of egg.
(2) reduce the excretion of phosphorus in animal wastes, slow down the pollution to environment for the phosphorus.The rivers of China, the rich battalion in waters
Fosterization pollution is extremely serious, such as Dianchi Lake Pollution, nearly red tide taking place frequently for 2 years, and in water body, excessive nitrogen and phosphorus are the masters polluting
Want reason, the phosphorus that wherein China discharges every year from feces of livestock and poultry reaches as many as 2,500,000 tons.Phytase using can by livestock and poultry and
In aquatic products excrement, the discharge rate of phosphorus reduces more than 40%, and that is, the discharge capacity of the annual phosphorus of China reduces about 1,200,000 tons.To significantly mitigate
The phosphorus pollution of the environment such as rivers, waters.In addition, phytase reduces the excretion of the heave metals trace elements such as copper, iron, zinc simultaneously；
In feed, plant recovery of nutrient improves, and the content of each organic matter of feces of livestock and poultry reduces, the environmental pollution being caused by the eutrophy of excrement
Mitigate, reduce the burst size of bad smell in animal barn, improve the environmental protection situation raising subenvironment.
(3) alleviate China's phosphorus source deficient, the under-supply situation of phosphorus.China is a Ge Que phosphorus big country, and phosphorus underproduces to be needed
/ 10th of the amount of asking, only feed phosphorus breach, just at 1,100,000 tons about, are only to be second to the second largest of protein feeds raw material to lack
Mouthful.Phytase use can reduce the Phos being added in feed, can use ten thousand tons of calcium monohydrogen phosphate 80-120 less every year, such that it is able to
Close down accordingly a collection of with serious pollution calcium monohydrogen phosphate factory, improve social benefit and ecological benefits.
(4) save phosphorous feed, the such as addition of calcium monohydrogen phosphate, bone meal etc., increase formula adjustment space, reduce feed and become
This.The use of phytase, 6～8 yuan/ton of perfect compound feed direct saving cost, total with 100,000,000 tons of feed of the current annual output of China
Yield, to calculate, can be increased farmers' income with 14.6 hundred million yuan of feed costs of direct saving, economic benefit highly significant.
Disclosed embodiment of this invention is the explanation to technical scheme it is impossible to conduct is to present invention
Restriction, those skilled in the art's simple change on the basis of the present invention, still within the scope of the present invention.
Claims (4)
1. a kind of application process in feed for phytase it is characterised in that: by the liquid phytase of preparation according to mass ratio 1:8
～15 spray on bentonite, prepare bentonite containing enzyme, will contain enzyme bentonite in feeding and feed with the uniform spice of pellet,
Spice mass ratio is 1:9800～10500；In described pellet the usage amount of inorganic phosphate be former usage amount 35%～
40%.
2. application process in feed for the phytase according to claim 1 it is characterised in that: the liquid of described preparation is planted
Sour enzyme refers to: aspergillus niger, saccharomycete, bacillus subtilis is put in culture medium, adds methyl alcohol as derivant, after fermentation both
, fermentation time 5～7d, temperature sets 28～30 DEG C.
3. application process in feed for the phytase according to claim 2 it is characterised in that: described culture medium refers to:
With following ratio, in 1000ml water, the soyabean cake diffusion juice of addition 10ml content 1%, 10g glucose, 3g peptone, 2.5g
Nacl, 2g caco3, 121 DEG C of moist heat sterilization 20min can inoculate.
4. phytase according to claim 1 in the application process in feed it is characterised in that: in described bentonite containing enzyme
Also comprise phytic acid enzyme stabilizers, using dark colored plastic bottle packaging.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611054016.6A CN106333084A (en) | 2016-11-25 | 2016-11-25 | Application method of phytase in feed |
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| CN201611054016.6A CN106333084A (en) | 2016-11-25 | 2016-11-25 | Application method of phytase in feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651758A (en) * | 2018-07-25 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | One kind chicken feed containing phytase and preparation method thereof |
| CN114958803A (en) * | 2022-05-18 | 2022-08-30 | 阳原县仁恒精细粘土有限责任公司 | Phytase fermentation production method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651758A (en) * | 2018-07-25 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | One kind chicken feed containing phytase and preparation method thereof |
| CN114958803A (en) * | 2022-05-18 | 2022-08-30 | 阳原县仁恒精细粘土有限责任公司 | Phytase fermentation production method |
| CN114958803B (en) * | 2022-05-18 | 2024-06-04 | 阳原县仁恒精细粘土有限责任公司 | Phytase fermentation production method |
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