CN106290500A - 利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法 - Google Patents

利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法 Download PDF

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CN106290500A
CN106290500A CN201610708981.4A CN201610708981A CN106290500A CN 106290500 A CN106290500 A CN 106290500A CN 201610708981 A CN201610708981 A CN 201610708981A CN 106290500 A CN106290500 A CN 106290500A
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perfluorinated sulfonic
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周京国
晏波
王东生
刑艳
青玉凤
蒋兴亮
杜琴
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Abstract

本发明公开了一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法，其特征在于：按下列步骤进行，a、制备石墨烯—二硫化钼—全氟磺酸树脂混合物；b、吸取G‑MoS2‑Nafion复合物酒精悬浊液0.005ml，滴加至玻碳电极表面，置于室温下自然干燥30min，即得到G‑MoS2‑Nafion修饰的玻碳电极；c、将上述玻碳电极置于PH=7.4的磷酸盐缓冲液中稳定2h；d、将分离好的血浆加入PBS中，使血浆正好被稀释5倍，搅拌20s后，利用三电极系统测定原理，计时电流法测定被稀释后血浆的电流值，经换算得出被检血浆中的尿酸浓度值。

Description

利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法
技术领域
本发明涉及本发明涉及电化学物质定量检测领域，尤其涉及一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法。
背景技术
现代医学证明，尿酸（uric acid，UA）既是人体内嘌呤代谢过程中的一种终产物，UA的存在对于人体新陈代谢具有重要作用，如作为神经传递素传递神经信号。另一方面，人体UA水平出现异常时对于某些疾病的诊断、治疗、监测和预防具有重要参考价值，如痛风、高尿酸血症、肾功能衰竭、尿路结石、高血压、冠心病、白血病、关节炎、Lesch-Nyhan综合症、精神分裂症和帕金森等。
目前UA检测尿酸的方法有酶法、分光光度测定法、高效液相色谱法等。1). 酶法是利用尿酸酶独特的分子识别能力和特异性催化作用，可以很好的排出其他物质的干扰，但其缺点是价格昂贵；2). 高效液相色谱法的优势是分离效果较好、快速、所用流动相简单，但对样品前处理步骤较为繁琐费时，在检测中有很多不便；3). 分光光度法是利用磷钨酸能被尿酸盐还原成蓝色磷钨酸复合物原理，通过光比色法来判断血尿酸含量，这种方法准确性好、操作简单，但缺点是灵敏度和特异性欠佳，以及对样品的预处理较为复杂，限制了其使用范围。总之上述UA检测方法存在灵敏度低、检测过程复杂、线性范围窄、检测成本高等主要缺陷。
发明内容
本发明的目的在于为了克服现有技术的不足而提供一种检测灵敏度高、线性范围宽、检测速度快、具有较强的抗干扰能力、检测成本低的一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法。其具体方法为：
a、制备石墨烯—二硫化钼—全氟磺酸树脂复合物（石墨烯与二硫化钼的质量比为5：2），称取氧化石墨烯（graphene oxide，GO）60mg，加入20mL蒸馏水，超声2h，使其形成浓度为3mg/mL的GO分散液；向GO悬浊水溶液中加入26.5mg钼酸铵和60mg硫代乙酰胺，再次超声30min；将上述制得的混合溶液倒入100mL聚四氟乙烯内衬不锈钢高压釜中，在200℃条件下恒温反应24h，反应结束后自然冷却至室温；取出反应釜，弃去上层滤热液，并将下层的沉淀转移至干净离心管中，分别用蒸馏水和无水乙醇交替洗涤3遍，最后将洗涤后的沉淀物置于冷冻干燥箱中进行干燥24h，即得G-MoS2；再称取G-MoS2 30mg，并加入15mL无水乙醇和0.01mL 全氟磺酸树脂（Nafion）溶液，混合后再次超声30min，即得到石墨烯—二硫化钼—全氟磺酸树脂（G-MoS2-Nafion）复合物酒精悬浊液；
b、吸取G-MoS2-Nafion复合物酒精悬浊液0.005ml，滴加至玻碳电极表面，置于室温下自然干燥30min，即得到G-MoS2-Nafion修饰的玻碳电极；
c、将上述玻碳电极置于PH=7.4的磷酸盐缓冲液（PBS）中稳定2h；
d、将分离好的血浆加入PBS中，使血浆正好被稀释5倍，搅拌20s后，利用三电极系统（玻碳电极、饱和甘汞电极、铂丝电极）测定原理，计时电流法（Amperometric i-t Curve，it）测定被稀释后血浆的电流值，经换算得出被检血浆中的尿酸浓度值。
通过以G-MoS2-Nafion为催化材料，以磷酸盐缓冲液（PBS，PH=7.4）为电解液，利用三电极系统（玻碳电极、饱和甘汞电极、铂丝电极）测定原理，线性扫描伏安法（linearsweep voltammetry，LSV）分析PBS、NaOH、葡萄糖、抗坏血酸和多巴胺对尿酸检测的干扰的试验表明。与现有技术相比，本发明的优势效果在于：1). 本发明具检测灵敏度高，提高了检测的可靠性；2). 检测线性范围，远超过了目前应用较为广泛的尿酸酶法；3). 检测速度快，每个样本的实际检测时间小于30s；4). 检测血浆尿酸浓度时，基乎不受其他物质（尤其是DA和AA）的影响，具有较强的抗干扰能力；5). 检测体系价格便宜，降低了检测成本；6).同时兼备G-MoS2-Nafion的制备过程简单，且可重复多次使用等优势。完全达到了本发明的发明目的。
附图说明
图1为本发明中制备的G-MoS2的X射线衍射图（X-ray diffraction，XRD）；
图2-1和图2-2分别为实施例中PBS、NaOH、Glu、AA、DA对UA测定的干扰情况和对人体血浆标本的检测情况；
图3-1和图3-2分别为实施例中不同尿酸浓度下对应的电流值及线性拟合结果；
图4为本发明方法对人体血尿酸的测定值与临床中尿酸酶法检测结果的相关性分析。
具体实施方式
实施例1，一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法。其具体方法为：
a、制备石墨烯—二硫化钼—全氟磺酸树脂复合物，称取氧化石墨烯（graphene oxide，GO）60mg，加入20mL蒸馏水，超声2h，使其形成浓度为3mg/mL的GO分散液；向GO悬浊水溶液中加入26.5mg钼酸铵和60mg硫代乙酰胺，再次超声30min；将上述制得的混合溶液倒入100mL高压反应釜中，在200℃条件下恒温反应24h，反应结束后自然冷却至室温；取出反应釜，弃去上层滤热液，并将下层的沉淀转移至干净离心管中，分别用蒸馏水和无水乙醇交替洗涤3遍，最后将洗涤后的沉淀物置于冷冻干燥箱中进行干燥24h，即得G-MoS2；再称取G-MoS230mg，并加入15mL无水乙醇和0.01mL 全氟磺酸树脂（Nafion）溶液，混合后再次超声30min，即得到石墨烯—二硫化钼—全氟磺酸树脂（G-MoS2-Nafion）复合物酒精悬浊液；
b、吸取G-MoS2-Nafion复合物酒精悬浊液0.005ml，滴加至玻碳电极表面，置于室温下自然干燥30min，即得到G-MoS2-Nafion修饰的玻碳电极；
c、将上述玻碳电极置于16mL PH=7.4的磷酸盐缓冲液（PBS）中稳定2h；
d、将分离好的血浆4mL加入PBS中，使血浆正好被稀释5倍，搅拌20s混匀后，利用三电极系统（玻碳电极、饱和甘汞电极、铂丝电极）测定原理，计时电流法（Amperometric i-tCurve，it）测定被稀释后血浆的电流值，经换算得出被检血浆中的尿酸浓度值。

Claims (4)

1.一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法，其特征在于：按下列步骤进行
a、制备石墨烯—二硫化钼—全氟磺酸树脂复合物，称取氧化石墨烯60mg，加入20mL蒸馏水，超声2h，使其形成浓度为3mg/mL的GO分散液；向GO悬浊水溶液中加入26.5mg钼酸铵和60mg硫代乙酰胺，再次超声30min；将上述制得的混合溶液倒入100mL聚四氟乙烯内衬不锈钢高压釜中，在200℃条件下恒温反应24h，反应结束后自然冷却至室温；取出反应釜，弃去上层滤热液，并将下层的沉淀转移至干净离心管中，分别用蒸馏水和无水乙醇交替洗涤3遍，最后将洗涤后的沉淀物置于冷冻干燥箱中进行干燥24h，即得G-MoS2；再称取G-MoS230mg，并加入15mL无水乙醇和0.01mL 全氟磺酸树脂溶液，混合后再次超声30min，即得到石墨烯—二硫化钼—全氟磺酸树脂复合物酒精悬浊液；
b、吸取G-MoS2-Nafion复合物酒精悬浊液0.005ml，滴加至玻碳电极表面，置于室温下自然干燥30min，即得到G-MoS2-Nafion修饰的玻碳电极；
c、将上述玻碳电极置于PH=7.4的磷酸盐缓冲液中稳定2h；
d、将分离好的血浆加入PBS中，使血浆正好被稀释5倍，搅拌20s后，利用三电极系统测定原理，计时电流法测定被稀释后血浆的电流值，经换算得出被检血浆中的尿酸浓度值。
2.根据权利要求1所述的一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法，其特征在于：利用G-MoS2-Nafion作为催化材料定量检测人体血尿酸的电化学方法。
3.根据权利要求1所述的一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法，其特征在于：其他物质对G-MoS2-Nafion作为电化学催化材料检测人体血尿酸的干扰测试分析。
4.根据权利要求3所述的一种利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法，其特征在于：向20mL的PBS电解液中依次加入0.01mL浓度分别为0.2mol/L的PBS、1mol/L的NaOH溶液、2mol/L的葡萄糖溶液、0.0001mol/L抗坏血酸、0.0001mol/L多巴胺、0.0001mol/L尿酸，根据所得LSV曲线图，分析上述物质对UA峰的电压位置及UA对应电流值的影响。
CN201610708981.4A 2016-08-24 2016-08-24 利用石墨烯—二硫化钼—全氟磺酸树脂检测尿酸的方法 Expired - Fee Related CN106290500B (zh)

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CN108717075A (zh) * 2018-05-21 2018-10-30 大连理工大学 利用电沉积全氟磺酸膜修饰的电极片检测尿酸的方法
CN109626435A (zh) * 2018-12-28 2019-04-16 哈尔滨理工大学 一种二硫化钼纳米片阵列/碳纳米纤维的制备及其应用
CN111304716A (zh) * 2020-03-12 2020-06-19 陕西煤业化工技术研究院有限责任公司 一种铅笔石墨修饰电极的制备方法和检测水体中苯二酚异构体的方法
CN113074998A (zh) * 2021-03-23 2021-07-06 毛江鸿 混凝土拌合物或海砂中氯离子浓度检测方法

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