CN106265120A - Cytokine-like effect of a kind of nine Oligopeptide Compositions and cosmetic applications - Google Patents
Cytokine-like effect of a kind of nine Oligopeptide Compositions and cosmetic applications Download PDFInfo
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- CN106265120A CN106265120A CN201610818082.XA CN201610818082A CN106265120A CN 106265120 A CN106265120 A CN 106265120A CN 201610818082 A CN201610818082 A CN 201610818082A CN 106265120 A CN106265120 A CN 106265120A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/31—Hydrocarbons
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/342—Alcohols having more than seven atoms in an unbroken chain
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The present invention relates to cosmetic technical field; disclose a kind of compound Oligopeptide Compositions for skin repair and application thereof; according to parts by weight, this Oligopeptide Compositions includes 1,1 ~ 30 part of tripeptides 1 bronze medal of 1 ~ 30 part of palmityl tripeptides, 5,1 ~ 30 part of carnosine of 1 ~ 50 part of palmityl tripeptides, 7,0.1 ~ 30 part of acetyl group hexapeptide of 5,1 ~ 30 part of Palmitoyl Tetrapeptide of 1 ~ 30 part of acetyl group tetrapeptide, 1,1 ~ 30 part of nisin of 8,0.1 ~ 30 part of nonapeptide.Oligopeptide Compositions has effect of cytokine-like, and affects the growth of skin flora, is applied on dermatology and beautifying skin, has and repairs skin lesion, adjusts metabolism and the effect of antagonism skin condition pathogenic bacterium growth.
Description
Technical field
The present invention relates to cosmetic technical field, particularly relate to cytokine-like effect of a kind of nine Oligopeptide Compositions with beautiful
Hold application.
Background technology
The polypeptide of molecular weight, the least peptide, oligopeptide, oligopeptide, or referred to as small active peptides, typically by several
Aminoacid forms.The oligopeptide of molecular weight, directly can be absorbed by skin.Along with medical science, threpsology, Dermatology and molecule are raw
The development of thing, oligopeptide is by increasingly in-depth study.Scientist finds, oligopeptide has various biology to Skin Cell
Activity, these biological active agents just more and more are disclosed out.
Have cytokine profiles in recent years and be applied to skin injury reparation and cosmetics, the red grade of He Zheng (1999) invention one
Plant cosmetic composition (containing human fibroblastic growth factor rh-bFGF, human vascular endothelial growth factor rh-VEGF, people's table
Skin cell growth factor rh-EGF, growth factor of human nerve rh-NGF and Melanin inhibitive factor MIF), said composition is in difference
Cellular metabolism and restoration updating is participated on level and different action site.He Zhenghong etc. (2001) invented a kind of medical science, beauty treatment,
And cosmetic composition, use the principle of antisensenucleic acids gene approach to block, close or reduce the synthesis of dermal melanin, press down
Cutaneous pigmentation processed.The production method of a kind of neurotrophic factor (NTF) of Zhou Shoushan etc. (2002) invention and application, this factor
There is good skin wrinkle removing effect.
Relative to peptide and proteins such as cell growth factor, the oligopeptide that molecular weight is little can have higher Cutaneous permeation
Property, it is easier to being absorbed by human body skin, arrived to a certain degree simultaneously because molecular weight is little, biological activity just there occurs flying of matter
Jump.Peptide molecular weight is the least, and " amino acid chain " is the shortest, is the most easily absorbed and utilized by the body.
Research to the mechanism of action of oligopeptide active substance finds, each oligopeptide has its specific biological effect.
They not only can affect cell proliferation, differentiation and the gene expression relevant with body growth growth, but also can be to Disease-causing gene
Express and produce important regulation effect.Can gene expression all levels (before transcribing, transcribe, transcribe after, translate and translate
After totally 5 levels) upper the most adjusted, although different oligopeptide are respectively arranged with its emphasis or single-minded regulation level, but most few
The regulation of gene expression is occurred on transcriptional level by peptide.
Main path has: (1) CAMP or CGMP protein kinase pathway；(2) tyrosine-kinase enzyme system, two above approach master
If by the phosphorylation of some transcription factor and/or cofactor and dephosphorylation, thus affect these factors
The activity that activated gene is transcribed；(3) ion channel；(4) approach that inosinyl phosphate inosine is acid mediated；(5) intracellular receptor approach, cell
Inner recipient can be the enzyme of catalytic reaction, it is also possible to being the tonality albumen of gene expression, most of active substances are to gene expression
Regulation and control realized by intracellular receptor approach.
The synergism that different action receptors makes somatomedin and little molecule victory peptide produce after compounding further promotes
Skin metabolism, speckle dispelling smoothing wrinkle, add the common effect of remaining composition in cosmetics, prevent skin aging, repair impaired
Skin, improves skin comprehensively, and compounding effect is significantly better than and is used alone.The current research report about this respect is the most very
Many, but each material owing to using is different, and content is inconsistent, and the effect that product is risen on skin is the most different；So, need
Want further research and probe can provide the Oligopeptide Compositions of effective skin repair comprehensively.
Summary of the invention
It is an object of the invention to provide a kind of compound Oligopeptide Compositions for skin repair.
It is a further object of the present invention to provide the application of this Oligopeptide Compositions.
For reaching above-mentioned purpose, the present invention by the following technical solutions:
A kind of compound Oligopeptide Compositions for skin repair, according to parts by weight, including 1 ~ 30 part of palmityl tripeptides-1,1 ~ 30
Part tripeptides-1 copper ,-5,1 ~ 30 part of carnosine of 1 ~ 50 part of palmityl tripeptides ,-5,1 ~ 30 part of Palmitoyl Tetrapeptide of 1 ~ 30 part of acetyl group tetrapeptide-
7,0.1 ~ 30 part of Argireline, 0.1 ~ 30 part of nonapeptide-1,1 ~ 30 part of nisin.
Oligopeptide can be to be modified by chemical method, such as acylated, soda acid.
Further, according to parts by weight, Oligopeptide Compositions includes-1,0.01 part of tripeptides-1 of 0.02 part of palmityl tripeptides
Copper ,-5,0.9 part of carnosine of 0.01 part of palmityl tripeptides ,-5,0.01 part of Palmitoyl Tetrapeptide-7 of 0.01 part of acetyl group tetrapeptide, 0.015 part
Argireline, 0.003 part of nonapeptide-1,0.18 part of nisin.
Further, described Oligopeptide Compositions also includes water, oils and fats, surfactant, wetting agent, antioxidant, anticorrosion
At least one in agent.
Further, described oils and fats be olive oil, castor oil hydrogenated, caprylic/capric triglyceride, Oleum Gossypii semen, soybean oil,
Oleum Hippophae, Jojoba oil, squalane, lanonol, cetearyl alcohol, polydimethylsiloxane, PSI, ring-type
At least one in polysiloxanes.
Further, described surfactant is polysorbas20, Tween 80, sodium laurate, stearyl alcohol sodium sulfate, polyoxyethylene
At least one in fatty acid ester, ceteareth-2, ceteareth-21, Phosphoric acid monohexadecyl ester.
Further, described wetting agent be glycerol, hyaluronate sodium, glycerine polyacrylate, oligofructose, xylitol,
At least one in propylene glycol, butanediol, methylglycine proline, glycine betaine.
Further, described antioxidant is vitamin C, vitamin E, riboflavin, nicotinic acid, metallothionein, super oxidation
Thing dismutase and, at least one in dimethylglycine.
Further, described preservative is methyl hydroxybenzoate, ethyl hydroxybenzoate, dimethyloldimethylhydantoin, iodine
At least one in propilolic alcohol butyl mephenesin Carbamate, DMDM Hydantoin, Methylisothiazolinone, phenoxyethanol.
The application in preparing cosmetics, skin care item or curable product of the above-mentioned Oligopeptide Compositions.
Further, the preparation type of described cosmetics, skin care item or curable product is essence, eye cream, cream, freezes
Dry powder, solution, Emulsion, cream, gel, facial film or dressing.
A kind of cream, including 5wt% Oligopeptide Compositions, 61.75wt% water, 5wt% glycerol, 3wt% propylene glycol, 0.15wt% card
Ripple nurse, 3wt% cetearyl glucoside, 2wt% PEG-100 stearate/glyceryl stearate, 1wt% cetearyl alcohol,
5wt% caprylic/capric triglyceride, 2wt% polydimethylsiloxane, 2wt% Butyrospermum parkii fruit fat, 0.5wt% tocopherol acetas,
8wt% 2-Methylpentadecane, 0.5wt% glycerol caprylate, 0.1wt% triethanolamine, 1wt%1,2-pentanediol, wherein, Oligopeptide Compositions is such as
Noted earlier.
A kind of Emulsion, including 5wt% Oligopeptide Compositions, 69.6wt% water, 3wt% glycerol, 5wt% propylene glycol, 0.1wt% card ripple
Nurse, 3wt% Glyceryl stearate citrate, 1.5wt% cetearyl alcohol, 3wt% caprylic/capric triglyceride, the poly-diformazan of 1wt%
Radical siloxane, 1wt% Butyrospermum parkii fruit fat, 0.2wt% tocopherol acetas, 6wt% 2-Methylpentadecane, 0.5wt% glycerol caprylate,
0.1wt% triethanolamine, 1wt%1,2-pentanediol, wherein, Oligopeptide Compositions is as previously described.
The method have the advantages that
The compound Oligopeptide Compositions of the present invention with the physiology of regulation of skin cell, and can stimulate the generation of corresponding Skin Cell interior
The cytokine of source property, such as h-bFGF, h-EGF, h-PDGF, h-aFGF, h-TGF-β, h-NTFs, h-KGF, h-VEGF etc., from
And skin actively repair procedure can be started, repair various types of skin problem and skin injury, and healthy skin has been had
Beautiful protective effect.
This Oligopeptide Compositions increases the growth of Skin Cell, strengthens skin cell metabolism, as collagen protein synthesis increases.
This Oligopeptide Compositions has the effect of antagonism skin condition pathogenic bacterium growth, such as suppression propionibacterium acnes, Fructus Vitis viniferae
Coccus, streptococcus growth, and adjust skin Tiny ecosystem.
This Oligopeptide Compositions has carnitine sample effect, reduces mediator transmission between neuromuscular, and has wrinkle removal effect.
This Oligopeptide Compositions has minimizing inflammatory mediator effect, alleviates inflammatory edema.
This Oligopeptide Compositions improves lymph circulation and microcirculation, adjusts skin condition in normally.
This Oligopeptide Compositions has reduction free radical, glycosylation and the effect of lipid oxidation, delay skin aging.
This Oligopeptide Compositions animal skin sensitivity tests, has no stimulation.
This Oligopeptide Compositions is easily obtained and preserves.
This Oligopeptide Compositions can directly smear face, it is also possible to the instrument conventional with beauty treatment and medical cosmetology and method, bag
The modes such as ultrasonic instrument, ion introducing apparatus, micropin, water laser accunputure, laser, injection that include introduce the composition into corresponding skin portion
Position.
This Oligopeptide Compositions can be prepared by conventional mixing, dissolving, emulsifying, liposomal, freeze drying technology etc..
The mechanism of action of these nine kinds of oligopeptide is:
Palmityl tripeptides-1 is derived from the fragment on I-type collagen chain, to skin cell renewal, repairs collagen protein synthesis tool
Have significantly regulation and control and facilitation, effectively facilitate collagen protein and the synthesis of hyaluronic acid in fibroblast, have crease-resistant,
Antiinflammatory, the effect of antiallergic.
Tripeptides-1 copper: copper binding peptide class, improves permeability, and allow original ionic forms is difficult to through stratum corneum barrier is inorganic
Copper ion can play effect by deep enough skin.Collagen protein, elastin laminin, hyaluronic acid, glycosaminoglycans and fiber can be improved even
The synthesis of desmin, for wound healing and hair growth, and has induced fibroblast secretion bFGF's and TGF-beta1
Effect.Collagen growth can be promoted, improve the synthesis capability of the skin moisture-keeping factor, slow down the snperoxiaized speed of skin lipid, improve
Skin heart microcirculation.Accelerate the reparation of skin trauma, reduce cicatrix, improve wrinkle, impaired skin is had powerful reparation merit
Effect, compact skin simultaneously, makes skin the most aobvious smooth bright；And the synthesis of collagen protein can be promoted, smooth microgroove and wrinkle.
Palmityl tripeptides-5: the particular sequence having is similar to human body body self mechanism, by activating inactive
TGF-β produce collagen protein, there is wrinkle removing effect.
Carnosine is Beta-alanine and the dipeptides of histidine composition.There is strong anti-oxidation, can remove in oxidative stress process
The fatty acid over oxidation of middle cell membrane and the reactive oxygen free radical that formed and alpha-beta unsaturated aldehyde；Significantly inhibit free radical and
The lipid oxidation that metal ion causes.
Acetyl group tetrapeptide-5 can improve the microcirculation of lymph and blood capillary, eliminates edema, alleviates black eye, improves skin
Skin is elastic and smoothness, also has the suppression glycosylated effect of collagen protein simultaneously, prevents that collagen protein is aging, assembles, thus reaches
To alleviating eye pouch and crease-resistant effect.
The fragment that Palmitoyl Tetrapeptide-7 is derived from immunoglobulin IgG, can significantly reduce inflammatory factor interleukin
The level of L-6, has the elimination potential inflammation of skin, the effect of antiallergic, produces alleviating wrinkle, recovers skin vigor.
Argireline is the N end imitating SNAP-25 albumen the oligopeptide being made up of six aminoacid, and its effect is permissible
Match in excellence or beauty with botulinum toxin type A, weaken muscle contraction, prevent the formation of wrinkle, but avoid botulinum toxin and must inject and make
By shortcoming with high costs.
Nonapeptide-1: this is a kind of bionical peptide, it has extraordinary matching, therefore with the MC1 receptor on melanocyte
Emulative can be combined with MC1 receptor as the antagonist of melanocyte-stimulating hormone, stop tryrosinase to be swashed further
Live and produce melanin.
Nisin is formed many by lactococcus lactis or a kind of of streptococcus acidi lactici secretion by 34 amino acid residues
Peptide matters, can suppress most gram positive bacteria, especially to produce spore gram positive bacteria such as bacillus subtilis, addicted to
Hot Bacillus stearothermophilus etc. have the strongest inhibitory action.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further:
In following example, palmityl tripeptides-1, tripeptides-1 copper, acetyl group tetrapeptide-5, Palmitoyl Tetrapeptide-7, Argireline
Purchased from the pharmacy of Zhuhai federation；Palmityl tripeptides-5, carnosine, nonapeptide-1 are purchased from Wei Qi bio tech ltd of Shenzhen；Breast chain
Bacterium peptide is purchased from Luoyang Qihong Biotechnology Co., Ltd..
Embodiment 1
The preparation of Oligopeptide Compositions
Palmityl tripeptides-1,100ppm solution, 200mL
Tripeptides-1 copper, 500ppm solution, 20mL
Palmityl tripeptides-5,1000ppm solution, 10mL
Carnosine, powder, 0.9g
Acetyl group tetrapeptide-5,1000ppm solution, 10mL
Palmitoyl Tetrapeptide-7,50ppm solution, 200mL
Argireline, 500ppm solution, 30mL
Nonapeptide-1,100ppm solution, 30mL
Nisin, 0.18g.
Above-mentioned oligopeptide is different according to dissolution properties, add water and be prepared as the solution of 1000mL.
Using above-mentioned solution as mother solution, it is acceptable that direct preparation joins cream, emulsion or other cosmetics and skincare products
In substrate；10 grams of mannitol can also be added in the solution according to lyophilization, divide according to 1 ~ 1.5 milliliter/cillin bottle
Dress, vacuum lyophilization, gland (seals), makes lyophilized powder.
Embodiment 2
Aging skin is crease-resistant, subtract wrinkle repair procedure
After conventional cosmetic care program, skin at wrinkle is sterilized with 70% medical alcohol, with the lyophilizing of embodiment 1 preparation
1, powder adds 1 ~ 3mL normal saline and dissolves, and this mixed liquor Direct Uniform is applied to skin, is massaged into and fully absorbs.Also may be used
With the instrument conventional with beauty treatment and medical cosmetology and method, including ultrasonic instrument, ion introducing apparatus or micropin, water laser accunputure,
The mode such as laser, injection imports, and helps skin absorption, to reach optimal skin repair effect.
Embodiment 3
Proliferation of human dermal fibroblasts activity test
Material
The raw material of embodiment 1, make 0.3 times, 1.0 times, 3.0 times of Oligopeptide Compositions standby, human skin fibroblast (Japan north
Gear division research department of seaway university), α-MEM culture medium (GIBCO), hyclone (GIBCO) mycillin (Invitrogen),
Trypan blue solution(Sigma), 0.25% Trypsin-1mM EDTA 4Na(Invitrogen), IMSO
(Sigma), cell number measures test kit WST-8(Japan) the full-automatic microplate reader of Infinite500 (Tecan).
Method and result
Human skin fibroblast is in MEM culture medium (containing 5% hyclone), 5%CO2, 95% humidity, the CO of 37 DEG C2In incubator
Cultivate, change liquid 1 time/3 days, cultivate the passage of 5 ~ 6 days, sucking-off original fluid, wash 2 times through PBS, add 0.25%
Trypsin-1mM EDTA 4Na solution 0.5mL, at 37 DEG C after 30 seconds, adds MEM culture medium 10mL, soft pressure-vaccum, makes thin
Born of the same parents suspend, and move to 50mL centrifuge tube, are centrifuged 2 minutes under 2000rpm, absorb supernatant, add appropriate containing blood serum medium, gently
Soft pressure-vaccum, makes cell even suspension.Take 96 orifice plates, every hole add cell suspension 100 μ L(containing 1000, cell), treat that cell is attached
After, add the compositions 10 μ L containing nine kinds of oligopeptide by group respectively, and with equal amount PBS 10 μ L as blank.Containing cell and
96 orifice plates of tester, cultivate 72 hours in incubator, take out 96 orifice plates, add WST-8 solution 10 μ L, again in each hole
Putting into and hatch colour developing in 4 hours in incubator, take out 96 orifice plates, measure OD value under 450nm, data press mean ± standard deviation table
Show, with SPSS 11.0 For Windows statistical software, data are carried out statistical procedures.Sample room comparison in difference t checks.
P < 0.05 indicates significant difference.Result is as follows:
The impact on proliferation of human dermal fibroblasts of nine kinds of Oligopeptide Compositions
| Test packet | n | OD |
| Comparison | 4 | 0.265±0.038 |
| 0.3 times of Oligopeptide Compositions | 4 | 0.685±0.057* |
| 1.0 times of Oligopeptide Compositions | 4 | 0.643±0.065* |
| 3.0 times of Oligopeptide Compositions | 4 | 0.626±0.083* |
* P < 0.01, the compositions test of three groups of oligopeptide is significantly higher than matched group, and i.e. the compositions of oligopeptide has increases into fiber finer
The effect of intracellular growth.
Embodiment 4
Collagen protein is tested
Material
The Oligopeptide Compositions of embodiment 1, human skin fibroblast (gear division research department of Hokkaido, Japan university), α-MEM cultivates
Base (GIBCO), hyclone (GIBCO) mycillin (Invitrogen), Trypan blue solution(Sigma),
0.25% Trypsin-1mM EDTA 4Na(Invitrogen), IMSO(Sigma), collagen protein test kit (Sircol(tm)
Soluble Collagen Assay, Biocolor).
Method and result
Take 96 orifice plates, add 1000 cells by every hole, within every 3 days, change liquid once, after cell attachment, each group is separately added into and contains
The composition solution 10 μ L of nine kinds of oligopeptide, and to add equal amount PBS as blank.After cultivating 7 days, by test kit description
Step measurements I-type collagen content.All data represent with mean ± standard deviation.Use SPSS 11.0 For Windows
Statistical software carries out statistical procedures.Sample room comparison in difference t checks, and P < 0.05 indicates significant difference, and result is such as
Under:
The impact that human skin fibroblast I-type collagen is secreted by the compositions of nine kinds of oligopeptide
| Matched group (n=4) | Oligopeptide Compositions test group (n=4) |
| 203.56±19.36ng/mL | 533.82±32.42ng/mL* |
* P < 0.01 liang of group significant difference, the compositions group of oligopeptide substantially increases the secretion of I-type collagen.
Embodiment 5
Skin surface bacterial count test
Material
The Oligopeptide Compositions of embodiment 1.Test reference strain: staphylococcus aureus (SA, this laboratory preserves), streptococcus
(SC, this laboratory preserves), propionibacterium acnes (PA, this laboratory preserves), colon bacillus (E.coli, this laboratory
Preserve).(formula: twist thin fresh beef 500g, adds triply distilled water 500mL, adds 1 M NaOH 25 Anaerobic Agar culture medium
ML, stirs, place 4 DEG C overnight, add pyrohydrolysis next day 1 hour, multilamellar cotton filter, to clarification filtrate in add pancreas egg
White peptone 30g, yeast powder 5g, K2HPO45g, 0.025% "diazoresorcinol" 4mL, add triply distilled water to 1000mL；Boil and cool down cultivation
Base, is placed in (80%N in anaerobic box2、10%H2、10%CO2), and add CYSTINE hydrochloric acid 0.5g, protoferriheme solution 10mL,
Vitamin K1 solution 0.2mL, its agar content is set as 1.5%).Anaerobic culture box (Shanghai).
Method and result
Experimenter 5 people, male 2 (30 years old and 45 years old), women 3 (22 years old, 25 years old and 30 years old).By experimenter wing of nose both sides
It is denoted as blank district, left side and the oligopeptide test section, right side of diameter 2 ~ 3cm.Operator carries sterile gloves, wipes with aseptic exempting from
Son dips physiological saline solution and Oligopeptide Compositions respectively, with first five day every day of sampling in wing of nose both sides respectively with normal saline and
Oligopeptide Compositions wiping repeatedly (about 10 times), cuts off shank by the cotton swab after wiping again in the 6th day (about 20 times), puts into 5mL
In normal saline.10 samples of sampling are done continuous 10 times of dilutions, is diluted to 10--6, take 10 respectively-3、10-4、10-5、10-6Dilute
Release liquid 0.1mL, be inoculated in respectively on Anaerobic Agar flat board with L rod, simultaneously with containing golden yellow coccus, streptococcus, acne propanoic acid bar
The bacterium solution of bacterium and colon bacillus is positive controls, and is separately added into Oligopeptide Compositions 10 μ L for positive test group, is placed in
Anaerobic culture box.After Anaerobic culturel 3-4 days, observed result, the clump count of counting growth.Result is as follows:
Oligopeptide Compositions affects (clump count cfu/mL) to the clump count of skin surface antibacterial and Reference Strains
| Group/dilution factor | 10-3 | 10-4 | 10-5 | 10-6 |
| Positive group | ||||
| SA | 160 | 20 | 3 | 0 |
| SA+ compositions | 93** | 7 | 0 | 0 |
| SC | 180 | 15 | 2 | 0 |
| SC+ compositions | 165 | 21 | 2 | 0 |
| PA | 190 | 25 | 3 | 0 |
| PA+ compositions | 115** | 15 | 0 | 0 |
| E.coli | 205 | 23 | 5 | 0 |
| E.coli+ compositions | 190 | 20 | 4 | 0 |
| Blank group (n=5) | 186.25±28.34 | 17.00±0.00 | - | - |
| Test group (n=5) | 103.31±21.36* | - | - | - |
* P < 0.05 compares with blank group, and clump count reduces meaningful.* positive control and SA and PA in positive test, prompting
A certain degree of clump count is had to reduce in the presence of compositions.-, clump count is few, and does not does statistical procedures.
Embodiment 6
Cutaneous sensibility is tested
Material
The Oligopeptide Compositions of embodiment 1.New Zealand white rabbit (2.36 ± 0.17kg) 8, each 4 of male and female, Zhongshan University's experiment is dynamic
Thing center provides.Normal saline.
Method and result
8 white rabbits are randomly divided into intact skin group and damaged skin group, often group 4, and every white rabbit sub-cage rearing, before test
Within 24 hours, at white rabbit spinal column lateral symmetry position, use operating scissors cropping, take off with depilatory (barium sulfate starch detergent 3:7:1)
Hair, depilation district area is each about 50cm in the every side of 10%(of body surface area2).In depilation district row once give tester and repeatedly to
Giving tester, observe skin wound repair intensity, both sides do nine kinds of Oligopeptide Compositions (left side) and normal saline (right side) respectively
Test.After skin depilatory 24 hours, checking and remove hair-fields skin integrity, damaged skin is unfit to do intact skin irritant test, broken
Damage the making of skin, use disposable sterilized No. 7 syringe needles, go hair-fields skin (through 75% alcohol disinfecting) to draw " # " word cut, broken
Damaging district area 3cm × 3cm, to stab epidermis, do not hinder corium, have oozing of blood degree of being, the damaged degree of the left and right sides should be basically identical.
The compositions of nine kinds of oligopeptide is smeared in depilation district, left side, and normal saline is smeared on right side, covers with two-layer gauze and smears position, with stingless
Swash property adhesive plaster to be fixed by gauze, then fix with mesh nylon tape.Every white rabbit sub-cage rearing, complete group of damaged combination respectively sets once
It is administered and multiple dosing, by upper method successive administration seven days, once-a-day, after giving tested material 24 hours, removes residual with warm water
Tested material, smears position with or without erythema and edema in perusal in 1,24,48 and 72 hours record.Result is as follows:
Skin irritation reaction scoring
Erythema: without erythema, score value: 0；The most visible, score value: 1；It is apparent that score value: 2；Moderate to Severe erythema, 3 parts；Purple
Red erythema also has eschar to be formed, score value: 4.
Edema: without edema, score value: 0；The most visible, score value: 1；Slight visible (edge exceeds surrounding skin), score value: 2；
Cutaneous protuberance about 1mm profile understands, score value: 3；Cutaneous protuberance about more than 1mm expanded range, score value: 4.
Erythema/edema
| Packet | 1 hour | 24 hours | 48 hours | 72 hours | Scoring |
| Intact skin group | |||||
| 1(single administration) | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0 |
| 2(single administration) | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0 |
| 3(multiple dosing) | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0 |
| 4(multiple dosing) | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0/0,0/0 | 0 |
| Damaged skin group | |||||
| 1(single administration) | 0/0,0/0 | 1/1,1/1 | 0/0,0/0 | 0/0,0/0 | 0.25 |
| 2(single administration) | 0/0,0/0 | 1/1,1/1 | 0/0,0/0 | 0/0,0/0 | 0.25 |
| 3(multiple dosing) | 0/0,0/0 | 1/1,1/1 | 0/0,0/0 | 0/0,0/0 | 0.25 |
| 4(multiple dosing) | 0/0,0/0 | 1/1,1/1 | 0/0,0/0 | 0/0,0/0 | 0.25 |
Intact skin and damaged skin, compared with matched group, be showed no the skin irritation of Oligopeptide Compositions by standards of grading.
Embodiment 7
The preparation (mass percent) of cream:
Water 61.75
Glycerol 5.00
Propylene glycol 3.00
Carbomer 0.15
Cetearyl glucoside 3.00
PEG-100 stearate/glyceryl stearate 2.00
Cetearyl alcohol 1.00
Caprylic/capric triglyceride 5.00
Polydimethylsiloxane 2.00
Butyrospermum parkii fruit fat 2.00
Tocopherol acetas 0.50
2-Methylpentadecane 8.00
Glycerol caprylate 0.50
Triethanolamine 0.10
The Oligopeptide Compositions 5.00 of embodiment 1
1,2-pentanediol 1.00
Preparation method:
1, Jiang Shui, glycerol, propylene glycol, carbomer stirring are warming up to 85 DEG C so that it is be completely dissolved, insulated and stirred 30 minutes；
2, by sweet to cetearyl glucoside, PEG-100 stearate/glyceryl stearate, cetearyl alcohol, caprylic/capric
Oil three esters, polydimethylsiloxane, Butyrospermum parkii fruit fat, tocopherol acetas, 2-Methylpentadecane, glycerol caprylate stirring are warming up to
80 DEG C so that it is be completely dissolved, insulated and stirred 20 minutes；
3, the mixture of step 2 is added in the mixture of step 1, stirring homogenizing 5 minutes, insulated and stirred 10 minutes；
4, stirring be cooled to 45 DEG C, add triethanolamine, 1,2-pentanediol, Oligopeptide Compositions stir 15 minutes, preparation completes.
Embodiment 8
The preparation (mass percent) of Emulsion:
Water 69.60
Glycerol 3.00
Propylene glycol 5.00
Carbomer 0.10
Glyceryl stearate citrate 3.00
Cetearyl alcohol 1.50
Caprylic/capric triglyceride 3.00
Polydimethylsiloxane 1.00
Butyrospermum parkii fruit fat 1.00
Tocopherol acetas 0.20
2-Methylpentadecane 6.00
Glycerol caprylate 0.50
Triethanolamine 0.10
1,2-pentanediol 1.00
The Oligopeptide Compositions 5.00 of embodiment 1
Preparation method:
1, Jiang Shui, glycerol, propylene glycol, carbomer stirring are warming up to 85 DEG C so that it is be completely dissolved, insulated and stirred 30 minutes；
2, by Glyceryl stearate citrate, cetearyl alcohol, caprylic/capric triglyceride, polydimethylsiloxane, Adeps Bovis seu Bubali
Fruit tree fruit fat, tocopherol acetas, 2-Methylpentadecane, glycerol caprylate stirring are warming up to 80 DEG C so that it is be completely dissolved, insulated and stirred
20 minutes；
3, the mixture of step 2 is added in the mixture of step 1, stirring homogenizing 5 minutes, insulated and stirred 10 minutes；
4, stirring be cooled to 45 DEG C, add triethanolamine, 1,2-pentanediol, Oligopeptide Compositions stir 15 minutes, preparation completes.
Embodiment 9
Formula and method according to embodiment 7 prepare cream, according to parts by weight, Oligopeptide Compositions by 1 part of palmityl tripeptides-1,
1 part of tripeptides-1 copper ,-5,1 part of carnosine of 1 part of palmityl tripeptides ,-5,1 part of Palmitoyl Tetrapeptide-7 of 1 part of acetyl group tetrapeptide, 0.1 part of acetyl
-8,0.1 part of nonapeptide-1 of base hexapeptide, 1 part of nisin composition.
Embodiment 10
Formula and method according to embodiment 8 prepare Emulsion, according to parts by weight, Oligopeptide Compositions by 30 parts of palmityl tripeptides-
1,30 parts of tripeptides-1 copper ,-5,30 parts of carnosines of 50 parts of palmityl tripeptides ,-5,30 parts of Palmitoyl Tetrapeptide-7 of 30 parts of acetyl group tetrapeptides, 30
Part Argireline, 30 parts of nonapeptides-1,30 parts of nisin compositions.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
Belong to those skilled in the art in the technical scope that the invention discloses, the change that can readily occur in or replacement, all answer
Contain within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with scope of the claims.
Claims (7)
1. the compound Oligopeptide Compositions for skin repair, it is characterised in that according to parts by weight, including 1 ~ 30 portion of Petiolus Trachycarpi
-1,1 ~ 30 part of tripeptides-1 copper of acyl tripeptides ,-5,1 ~ 30 part of carnosine of 1 ~ 50 part of palmityl tripeptides, 1 ~ 30 part of acetyl group tetrapeptide-5,1 ~ 30
Part Palmitoyl Tetrapeptide-7,0.1 ~ 30 part of Argireline, 0.1 ~ 30 part of nonapeptide-1,1 ~ 30 part of nisin.
Oligopeptide Compositions the most according to claim 1, it is characterised in that according to parts by weight, including 0.02 part of palmityl
Tripeptides-1,0.01 part of tripeptides-1 copper ,-5,0.9 part of carnosine of 0.01 part of palmityl tripeptides, 0.01 part of acetyl group tetrapeptide-5,0.01 part
Palmitoyl Tetrapeptide-7,0.015 part of Argireline, 0.003 part of nonapeptide-1,0.18 part of nisin.
Oligopeptide Compositions the most according to claim 1, it is characterised in that described Oligopeptide Compositions also includes water, oils and fats, table
Face activating agent, wetting agent, antioxidant, at least one of the preservatives.
4. the answering in preparing cosmetics, skin care item or curable product of the Oligopeptide Compositions described in any one of claim 1 ~ 3
With.
Application the most according to claim 4, it is characterised in that the preparation of described cosmetics, skin care item or curable product
Type is essence, eye cream, cream, lyophilized powder, solution, Emulsion, cream, gel, facial film or dressing.
6. a cream, it is characterised in that include 5wt% Oligopeptide Compositions, 61.75wt% water, 5wt% glycerol, 3wt% propylene glycol,
0.15wt% carbomer, 3wt% cetearyl glucoside, 2wt% PEG-100 stearate/glyceryl stearate, 1wt% spermaceti
Stearyl alcohol, 5wt% caprylic/capric triglyceride, 2wt% polydimethylsiloxane, 2wt% Butyrospermum parkii fruit fat, 0.5wt% tocopherol
Acetas, 8wt% 2-Methylpentadecane, 0.5wt% glycerol caprylate, 0.1wt% triethanolamine, 1wt%1,2-pentanediol, wherein, oligopeptide
Compositions is as claimed in claim 2.
7. an Emulsion, it is characterised in that include 5wt% Oligopeptide Compositions, 69.6wt% water, 3wt% glycerol, 5wt% propylene glycol,
0.1wt% carbomer, 3wt% Glyceryl stearate citrate, 1.5wt% cetearyl alcohol, 3wt% caprylic/capric triglyceride,
1wt% polydimethylsiloxane, 1wt% Butyrospermum parkii fruit fat, 0.2wt% tocopherol acetas, 6wt% 2-Methylpentadecane, 0.5wt% are sweet
Oil caprylate, 0.1wt% triethanolamine, 1wt%1,2-pentanediol, wherein, Oligopeptide Compositions is as claimed in claim 2.
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