CN107375048A - Lightening compositions and its application - Google Patents
Lightening compositions and its application Download PDFInfo
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- CN107375048A CN107375048A CN201710581552.XA CN201710581552A CN107375048A CN 107375048 A CN107375048 A CN 107375048A CN 201710581552 A CN201710581552 A CN 201710581552A CN 107375048 A CN107375048 A CN 107375048A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a kind of lightening compositions, said composition includes active ingredient：Yeast extract and undaria pinnitafida extract solution.To improve whitening effect, active ingredient palmityl tripeptides 5 have been can further include in said composition.The invention also discloses application of the above-mentioned lightening compositions in skin-lightening cosmetic is prepared.The present invention suppresses tyrosinase activity by many-side, reduce the generation and transfer of skin pigment, promote collagen synthesis, delay skin aging, quickly, the effect of skin whitening has been reached safely, effectively, especially undaria pinnitafida extract solution, lightening compositions associated with 5 three kinds of whitening compositions of yeast extract and palmityl tripeptides, have stronger collaboration white-skinned face function.
Description
Technical field
The present invention relates to cosmetic field, more particularly to a kind of skin lightening compositions and its is preparing skin-whitening
Application in cosmetic.
Background technology
The color of human skin is mainly regulated and controled by the melanocyte in epidermis, and melanocyte produces melanin, black
Crude granule is shifted by the dendritic projection of melanocyte to keratinocyte, is gathered on keratinocyte core, then, black
Pigment granule goes upward to cuticula with epidermal cell, so as to cutaneous color or color spot is formed, finally with epidermal cell
Come off and remove.In melanocyte, melanin synthesizes in melanosome.Tyrosine is oxidized to DOPA by tyrosinase
Quinone, and continue oxidative deamination, after forming dihydroindole, it is changed into melanin.Tyrosinase is the main speed limit of B16 cell
Enzyme.
Victory peptide is also known as polypeptide or peptide, and simpler than protein structure, molecular weight is small, is to be connected by 2-16 amino acid by peptide bond
A kind of compound connect, small peptide (2-5 amino acid) and polypeptide (6-16 amino acid) can be divided into according to amino acid composition number.
The less victory peptide of molecular weight, can directly be absorbed by skin.Scientist find, victory peptide have to Skin Cell anti-aging, it is anti-oxidant,
The biological activities such as anti-inflammatory, antibacterial, whitening.Wherein, with white-skinned face function victory peptide mainly have tetrapeptide -30, acetyl hexapeptide -1 and
Nonapeptide -1.
Palmityl tripeptides -5 (palmitoyl-K-V-K) are a kind of signal peptides of synthesis, can simulate platelet response egg
White 1 sequence, cause sequence KRFK to be tied to sluggish TGF β, cause active TGF β release, promote collagen generation.
Human Clinical Study is shown, compared with control group, 60 healthy volunteers use 2.5% and 1% palmityl tripeptides -5 daily, often
It twice, continuous 84 days.Average wrinkle parameter can be significantly reduced, maximum can reach 12%.Have been reported that display palmityl-
KVK-L- ascorbic acid, the generation of melanin in melanocyte can be suppressed, after human clinical trial uses 12 weeks, skin wrinkle
Line reduces the colour of skin and highlighted.Ascorbic acid is known whitening agent, does not refer to whether palmityl-KVK has whitening effect in article
Fruit.
A kind of entitled " cytokine-like effects and cosmetic applications of nine Oligopeptide Compositions " (publication number：
CN106265120A Chinese patent application), one kind is disclosed by palmityl tripeptides -1, the copper of tripeptides -1, palmityl tripeptides -5, flesh
There is the composition of the oligopeptides such as peptide, acetyl group tetrapeptide -5, nonapeptide -1 composition reparation skin lesion, adjustment metabolism and antagonism skin condition to cause
The effect of pathogen growth.Nonapeptide -1 is a kind of bionical peptide, and it has extraordinary matching with the MC1 acceptors on melanocyte,
It can competitively be combined with MC1 acceptors, prevent tyrosinase from being further activated and produce melanin.The copper of tripeptides -1, tripeptides -
1 is combined with copper, promotes the permeability of copper ion, being capable of promotion organization reparation.There are some researches prove blue copper peptide can promote at scar
Aggregation extra collagen degradation, promote normal skin in collagen synthesis, promote elastin laminin, proteoglycans,
Glucose aminoglucan generates, while is also equipped with anti-inflammatory antioxidation.But other oligopeptides are not referred in the patent application,
Such as palmityl tripeptides -5 and the copper of tripeptides -1, if having whitening function.
Undaria pinnitafida (Undaria pinnatifida) is large ocean brown alga, belongs to Phaeophyta, brown sub- guiding principle, Laminariales, wing algae
Section.Polysaccharide, protein and trace element are rich in undaria pinnitafida.Undaria pinnitafida has anti-inflammatory, anti-oxidant, hypotensive, antiviral, fat-reducing
And other effects.Studies have reported that the ethyl acetate extract of undaria pinnitafida, under 3.125 μ g/ml concentration, by lowering MITF expression,
The melanin excessively generation and tyrosinase activity that α-MSH inductions can be significantly inhibited rises.Undaria pinnitafida extract also has very
Good white-skinned face function.
Publication No. CN105232395A Chinese patent application, disclose one kind is with oriental cherry, undaria pinnitafida, broom Chinese photinia etc.
The novel whitening toner of main composition.Sample inhibitory activity against tyrosinase by compounding is 61.3%, than 3%
The inhibitory activity against tyrosinase 6.5% of undaria pinnitafida extract is obviously improved.But melanin content is not investigated in the patent application,
Therefore fail directly to prove to cooperate with white-skinned face function associated with undaria pinnitafida and other extracts.
Contain various natural components such as amino acid, polypeptide, mineral matter etc. in yeast extract, various oligopeptides constituents can be with
Various biological respinses are participated in as signaling molecule.(skin whitening efficacy of pure natural yeast extract, the detergents and cosmetic such as Zhao little Min
Industry, 2011,41 (4)：268-271) confirmed by human clinical trial, it is aobvious using yeast extract 42 days, skin brightness L values
Write and increase by 30%, dermal melanin index M I values are remarkably decreased 38%, have good white-skinned face function.Yeast extract is as day
So the active matter of safety is had been widely used in cosmetics.Publication No. US2011052517A1 U.S. Patent application, it is public
Open peptide caused by yeast (Phe-Val-Ala-Pro-Phe-Pro) to be complexed with divalent metal, product can be independent of suppression junket
Propylhomoserin enzymatic activity reduces B16 cell, enters chromatophore by suppressing calcium current to reduce B16 cell.As can be seen here, yeast
Extract can play whitening function by suppressing tyrosinase activity, suppressing calcium current and the various ways such as enter.
Publication No. KR20120077271A korean patent application discloses the opotism to be fermented through zymogenous bacterias such as yeast
The extracts such as dish can promote the growth of eyelash.Publication No. KR20110075626A korean patent application is disclosed through ferment
The zymogenous bacterias such as mother and the tunning of undaria pinnitafida extract can improve skin regeneration ability.But undaria pinnitafida extract with
Whitening effect does not have been reported that associated with yeast extract.
The content of the invention
One of the technical problem to be solved in the present invention is to provide a kind of lightening compositions, and it can safely, effectively whitening
Human body skin.
In order to solve the above technical problems, the lightening compositions of the present invention, include yeast extract and undaria pinnitafida extract solution.
Accounting of the yeast extract in the lightening compositions gross mass is preferably 5%, the undaria pinnitafida extraction
Accounting of the liquid in the lightening compositions gross mass is preferably 3%.
The lightening compositions can also include the following components of the percentages by composition gross mass：Carbomer
0.4%, Phenoxyethanol 0.5%, xanthans 0.6%, mass percent concentration is 1wt% NaOH 9.8%.
Preferably, the mother liquor of palmityl tripeptides -5 is may further include in the lightening compositions, with yeast extract
It is combined with undaria pinnitafida extract solution.The mass percent concentration of the mother liquor of palmityl tripeptides -5 is 0.1wt%, in the whitening group
Accounting in compound gross mass is preferably 3%.
Preferably, the mother liquor of nonapeptide -1 is may further include in the lightening compositions, with yeast extract and opotism
Dish extract solution is combined.The mass percent concentration of the mother liquor of nonapeptide -1 is 0.01wt%, in the lightening compositions gross mass
In accounting be preferably 8%.
Preferably, the copper mother liquor of tripeptides -1 is may further include in the lightening compositions, with yeast extract and skirt
The extract solution combination of band dish.The mass percent concentration of the copper of tripeptides -1 mother liquor is 1wt%, in the lightening compositions gross mass
In accounting be preferably 10%.
Preferably, the above-mentioned mother liquor of palmityl tripeptides -5, the mother liquor of nonapeptide -1, the copper of tripeptides -1 mother liquor can also combination of two or three
After kind mother liquor combination, it is combined with yeast extract and undaria pinnitafida extract solution.
The second technical problem to be solved by the present invention is to provide above-mentioned lightening compositions answering in skin-lightening cosmetic is prepared
With.
When preparing skin-lightening cosmetic, the lightening compositions can be used alone, can also with other functional components or
Cosmetic compounding use.The form of skin-lightening cosmetic can be face cleaning, emulsion, cream, elite etc., be used to outer painting skin
Whitening.
The lightening compositions of the present invention, tyrosinase activity is suppressed by many-side, reduce the generation of skin pigment and turn
Move, promote collagen synthesis, delay skin aging, effect that is quick, safely, effectively having reached skin whitening, compared to existing
Some whitening products, lightening compositions of the invention are safer effectively to the whitening function of human body skin, especially undaria pinnitafida
Lightening compositions associated with three kinds of extract solution, yeast extract and palmityl tripeptides -5 whitening compositions, have stronger collaboration beautiful
Contour painting is imitated, and exceeds well over the whitening effect of any of which single component.
Embodiment
More specifically understand to have to technology contents, feature and effect of the present invention, existing specific embodiment, to the present invention's
Technical scheme is further described in detail.
First, reagent
α-MSH (α-melanocyte simulating hormone), ursin, Mushroom Tyrosinase, L-DOPA：Purchase
From Sigma companies
Tetrazole indigo plant (MTT)：Produced by Sigma companies
DMEM nutrient solutions：Produced by Wisent companies
D-Hanks buffer solutions：Produced by Gibco companies
Hyclone：Produced by PAN Biotech companies
Green grass or young crops-streptomysin：Produced by Hyclone companies
Yeast extract：Purchased from Angel Yeast Co., Ltd, solid content 5%.
Undaria pinnitafida extract solution：Chemical Co., Ltd., solid content 1.8% are avenged purchased from Guang Zhou Austria.
The sterling of palmityl tripeptides -5：Purchased from DSM Co., Ltd
The sterling of nonapeptide -1：Purchased from Zhuhai pharmaceutical Co. Ltd of federation
The copper sterling of tripeptides -1：Purchased from Shanghai Xian En Co., Ltds
Above reagent is commercially produced product.
(mass percent concentration is for the mother liquor of palmityl tripeptides -5 (mass percent concentration 0.1%), the mother liquor of nonapeptide -1
0.01%), the copper of tripeptides -1 mother liquor (mass percent concentration 1%) is respectively by the sterling of palmityl tripeptides -5, the sterling of nonapeptide -1, three
The copper sterling of peptide -1, which is dissolved in deionized water, to be formulated.
2nd, instrument
SH-4B UV-B type ultraviolet radiation meters：Purchased from Xigema High Technology Co., Ltd., Shanghai
NH310 color difference meters：Purchased from En Chi Science and Technology Ltd.s of Shenzhen three
Multi-function microplate reader：Produced by Thermo companies
CO2Incubator：Produced by Nuaire companies of the U.S.
Inverted microscope：Produced by German Leica companies
Super-clean bench：Purchased from Purifying Equipment Co., Ltd., Suzhou
Ultrasonic cell disruption instrument：Purchased from Xin Zhi biotech firms
The digital display overhead stirrer of Eurostar 60, produced by German IKA companies.
3rd, experimental animal
Pattern cavy：Purchased from Shanghai Jia Gan bio tech ltd
Embodiment 1
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：The mother liquor of 3% palmityl tripeptides -5
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 85.7% water
C：9.8% 1%NaOH
Above-mentioned B, component C effect are thickening, anti-corrosion.
The preparation method of the lightening compositions is：B phase raw materials are pre-dissolved, 300r/min is stirred under normal temperature, in stirring
Under be slowly added to A phase whitening components, after stirring, be slowly added to C phases.
Embodiment 2
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 83.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 3
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：3% undaria pinnitafida extract solution
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 85.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 4
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract, the mother liquor of 3% palmityl tripeptides -5
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 80.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 5
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：3% undaria pinnitafida extract solution, the mother liquor of 3% palmityl tripeptides -5
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 82.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 6
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract, 3% undaria pinnitafida extract solution
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 80.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 7
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract, 3% undaria pinnitafida extract solution, the mother liquor of 3% palmityl tripeptides -5
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 77.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 8
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：The mother liquor of 3% palmityl tripeptides -5, the copper mother liquor of 10% tripeptides -1, the mother liquor of 8% nonapeptide -1
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 67.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 9
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract, 3% undaria pinnitafida extract solution, the copper mother liquor of 10% tripeptides -1
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 70.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 10
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：5% yeast extract, 3% undaria pinnitafida extract solution, the mother liquor of 8% nonapeptide -1
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 72.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 11
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：The copper mother liquor of 10% tripeptides -1
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 78.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Embodiment 12
The lightening compositions of the present embodiment, (by percentage to the quality) composed of the following components：
A (whitening component)：The mother liquor of 8% nonapeptide -1
B：0.4% carbomer, 0.5% Phenoxyethanol, 0.6% xanthans, 80.7% water
C：9.8% 1%NaOH
The preparation method of the lightening compositions is the same as embodiment 1.
Influence of the 13 different whitening active ingredients of embodiment to B16 melanoma cells B16 cells
With the DMEM cell culture fluids comprising 10% hyclone, 100U/mL penicillin and 100U/mL streptomysins by B16
The density of melanocyte (being purchased from Shanghai Fu Xiang bio tech ltd) is adjusted to 2 × 104Individual/mL, it is inoculated in every hole 3mL
In 6 porocyte culture plates, in 37 DEG C, 5%CO2Under the conditions of cultivate 24h it is adherent after, give 1 μM of α-MSH, and give various concentrations
Testing sample.After acting on 72h, abandon supernatant and washed with PBS, 0.5mL trypsin digestion cell 3min, addition are then added per hole
2mL cell culture fluids terminate digestion reaction, count every group of cell.After centrifugation, supernatant is abandoned, adds 1mol/L NaOH solutions
0.5mL, after 60 DEG C are boiled 30min, 1500r/min centrifugation 5min, 200 μ L are taken to move on to 96 porocyte culture plates respectively, with enzyme mark
Instrument detects 450nm absorbance.Melanin content is calculated, calculation formula is：
Melanin content=[(A/B)/(C/D)] × 100%
In formula, A is medicine hole absorbance, and B is medicine hole cell number, and C is control wells absorbance, and D is control wells cell number.
As a result it is as shown in table 1.
1 different whitening active ingredients of table to B16 melanoma cells B16 cells influence (n=4,)
(*：P ＜ 0.01VS control groups；#：P ＜ 0.05VS model groups；##：P ＜ 0.01VS model groups)
By table 1, it can be seen that, palmityl tripeptides -5 can significantly reduce the melanin content in B16 melanoma cells,
And with the rising of the concentration of palmityl tripeptides -5, the content of melanin further reduces, under 0.001% concentration, melanin
Content is 81.4% (P ＞ 0.05), and under 0.0001% concentration, melanin content is 86.9% (P ＞ 0.05), and melanin is contained
The reducing effect of amount exceedes nonapeptide -1 and the copper of tripeptides -1 under same concentrations.Yeast extract and undaria pinnitafida extract solution also show good
The good effect for suppressing melanin generation.
Influence of the 14 different whitening active ingredients of embodiment to tyrosinase activity in B16 melanoma cells
It is with the DMEM cell culture fluids containing 10% hyclone, penicillin 100U/mL and streptomysin 100U/mL that B16 is black
Chromatophore density is adjusted to 1 × 105Individual/mL, it is inoculated in every hole 3mL in 6 porocyte culture plates, in 37 DEG C, 5%CO2Under the conditions of
Testing sample is added after culture 24h.After medicine acts on 3 days, reject supernatant, rinsed 2 times with PBS.180 μ L are added per hole to contain
After 1%Triton X-100 PBS solution, ultrasonication in ice bath adds the μ L 10mmol/L of people 20 L-DOPA per hole, 37 DEG C
60min, 1500r/min centrifugation 5min are incubated, takes 100 μ L to move on to 96 porocyte culture plates, ELIASA detection 492nm extinctions respectively
Angle value, calculates intracellular tyrosine enzymatic activity, and calculation formula is as follows：
Tyrosinase activity=(A/B) × 100%
In formula, A is medicine group mean light absorbency, and B is control group mean light absorbency.
Experimental result is as shown in table 2.
2 different whitening active ingredients of table to tyrosinase activity in B16 melanoma cells influence (n=4,)
(**：P ＜ 0.01VS control groups；#：P ＜ 0.05VS model groups；##：P ＜ 0.01VS model groups)
By table 2 it can be seen that, with the rising of the concentration of palmityl tripeptides -5, tyrosinase in B16 melanoma cells
Active gradually to reduce, tyrosinase activity is 81.3% (P ＞ 0.05) under 0.001% concentration, and action effect exceedes same concentrations
Under nonapeptide -1 and the copper of tripeptides -1, also superior to yeast extract and undaria pinnitafida extract solution.As can be seen here, palmityl tripeptides -5 can
By suppressing tyrosinase activity, suppress the generation of melanin in melanocyte.
Influence of the embodiment 1-12 of embodiment 15 lightening compositions to guinea pig skin brightness
Tested by cell screening, it has been found that palmityl tripeptides -5 have the function that the good melanin that suppresses generates.
Therefore, further investigate embodiment 1-10 and cause the action effect on guinea pig skin pigmentation model in UVB.
The male brown color cavy 72 of health, 10 week old, 240~280g of body weight, adaptability are raised one week, are randomly divided into 12
Group, every group 6.First cut off guinea pig back with scissors to become mildewed, pruning region area 6cm2×6cm2, then shaved with electric shaver
Undercoat to the greatest extent, UVB ultra violet lamp guinea pig backs stripped region, fluorescent tube is apart from back 15cm, 280~320nm of ultraviolet wavelength, irradiation
Total amount 500mJ/cm2, the next day 1 time, totally 3 times.The external used medicine 1 week since after the 3rd irradiation, every three days shavings before medicine-feeding
Once, daily medication 2 times, was substantially assessed when 1 week, 2 weeks, 3 weeks after pre-irradiation and medication, color difference meter detection L values,
Tissue biopsy is done after 3 weeks, and makees Microscopic observation analysis.
Skin color determines：When respectively after UVB pre-irradiations, irradiation 1 week, 2 weeks and 3 weeks after 1 week and medication, color difference meter is used
Measure the L value changes in each region of guinea pig back skin.Calculation formula is as follows：
The L values of skin after L values-irradiation of L value changes value (△ L)=pre-irradiation skin
After experiment terminates, take irradiated site skin to fix, carry out Fontana Masson dyeing, statistical analysis melanin point
The gray value at cloth position.Calculation formula is as follows：
Pathological staining melanin Germ distribution gray value=Germ distribution gray value-background gray levels
Mapped using the medical science mapping softwares of GraphPad Prism 5.0, SigmaStat softwares carry out data analysis.It is all
Data represent that △ L use replication two-way analysis of variance with means standard deviation, compare between group and are divided using SNK Test
Analysis is examined.
Synergy calculates according to Jin's formula：
Q=E (A+B)/[EA+EB-EA*EB]
EA and EB is effect when A and B is used alone in formula；E (A+B) is 2 effects shared.
Q=0.85~1.15 are added to be simple；1.15 ＜ q ＜ 20 are enhancing；Q ＞ 20 are to be remarkably reinforced；Q ＜ 0.56~
0.85 is antagonism；Q ＜ 0.55 are obvious antagonism.
Result of the test is as shown in Table 3-6.
From table 3,4, embodiment 1-12 lightening compositions can significantly improve ultraviolet caused mouse skin color
It is plain calm, significantly reduce the melanin in mouse skin.Wherein, the △ L values of embodiment 4~6 and melanin gray value are below
Embodiment 1~3, especially using the value of embodiment 6 to be minimum, it is seen then that -5 points of yeast extract, undaria pinnitafida extract solution and palmityl tripeptides
Whitening effect after other combination of two is better than the whitening effect that every kind of composition is used alone；And the yeast extract of embodiment 6 and
The whitening effect of undaria pinnitafida extract solution combination is optimal in embodiment 4~6, shows, yeast extract and undaria pinnitafida extraction liquid energy
Enough collaborations play more preferable whitening function, and this can also be confirmed from table 5,6.
Embodiment 7~10 is being applied in combination for three kinds of raw materials, and the △ L values and melanin gray value of embodiment 7,9,10 are less than
Embodiment 6, wherein again using the value of embodiment 7 to be minimum, but only palmityl tripeptides -5 can lift yeast extract, opotism
The white-skinned face function of dish extract solution combination, coefficient of concordance 1.56, and the copper of tripeptides -1 and nonapeptide -1 carry with yeast extract, undaria pinnitafida
Take liquid combination to have antagonism (coefficient of concordance is respectively 0.56,0.58, and ginseng is shown in Table 6).
Embodiment 7 has three kinds of combinations, is calculated by coefficient of concordance as can be seen that △ L values, the collaboration of every kind of combination in table 5
Coefficient is all higher than 1.15, is up to 2.64；Skin gray value in table 6, the coefficient of concordance of every kind of combination are all higher than 1.15, are up to
1.80.It can be seen that three combines whitening function enhancing.
The embodiment 1-12 of table 3 lightening compositions to guinea pig skin color influence (n=6,)
| Group | △ L values |
| Model group | 6.74±0.70 |
| 1% ursin | 4.33±2.03* |
| Embodiment 1 | 5.40±1.51* |
| Embodiment 2 | 6.18±1.02* |
| Embodiment 3 | 5.22±1.62* |
| Embodiment 4 | 5.02±0.33* |
| Embodiment 5 | 4.97±1.76* |
| Embodiment 6 | 4.82±1.60* |
| Embodiment 7 | 1.37±0.17** |
| Embodiment 8 | 5.38±2.28* |
| Embodiment 9 | 2.51±1.24** |
| Embodiment 10 | 2.68±1.32* |
| Embodiment 11 | 5.41±2.62* |
| Embodiment 12 | 5.52±2.31* |
(*：P ＜ 0.05VS model groups；**：P ＜ 0.01VS model control groups)
The embodiment 1-12 of table 4 to guinea pig skin melanin Germ distribution gray value influence (n=6,)
| Group | Gray value |
| Model group | 92.4±6.3 |
| 1% ursin | 63.1±8.3** |
| Embodiment 1 | 82.7±1.6* |
| Embodiment 2 | 75.3±5.4** |
| Embodiment 3 | 78.8±6.4** |
| Embodiment 4 | 73.8±8.2* |
| Embodiment 5 | 72.5±4.7** |
| Embodiment 6 | 65.1±7.0** |
| Embodiment 7 | 39.3±8.8** |
| Embodiment 8 | 76.4±1.3* |
| Embodiment 9 | 67.8±7.3* |
| Embodiment 10 | 66.2±5.7* |
| Embodiment 11 | 68.3±4.5* |
| Embodiment 12 | 67.4±9.1* |
(*：P ＜ 0.05VS model groups；**：P ＜ 0.01VS model control groups)
Influences of the embodiment 1-12 of table 5 to ultraviolet irradiation guinea pig skin color synergy (n=6,)
(*：P ＜ 0.05VS model groups；**：P ＜ 0.01VS model control groups)
The embodiment 1-12 of table 6 on guinea pig skin melanin Germ distribution gray value influence synergy (n=6,)
(*：P ＜ 0.05VS model groups；**：P ＜ 0.01VS model control groups)
Whitening effect of the lightening compositions of embodiment 16 to human body skin
Using the whitening effect of the double-blind study test evaluation present invention, the people of subject 25, every group of 5 people.Whole face as by
Position is tried, gives embodiment 1,2,3,6,7 respectively, is used daily twice, once in the morning and once at night.Continuous use 4 weeks, using preceding and 4
Skin color test (Chromameter, CR-400, Minolta) is carried out after week, each test section is tested 3 times and averaged.
Measurement result is with International Commission on Illumination (Commission International del Eclairage, CIE)
L*a*b* color systems are represented (referring to table 7).Wherein：
L* values represent balance from white to black, and ater 0, pure white 100, L* values are bigger, closer to white,
L* values are smaller, closer to black.
A* values represent the balance from red to green, and its excursion is+60~-60, on the occasion of expression red direction, negative value
Represent green direction.
B* values represent the balance from yellow to blueness, and its excursion is+60~-60, on the occasion of expression yellow direction, negative value
Represent blue direction.
ITA ° of value, ITA ° of value table are calculated by ITA °=[Arc Tan ((L*-50)/b*)] × 180/3.14159 of formula
Show skin color, numerical value is higher, and skin is whiter.
Whitening effect of the lightening compositions of table 7 to human body skin
(compared with 0 week, *:P ＜ 0.05, difference has statistical significance)
As seen from Table 7, after using product 4 weeks, the L* values of each group face are above basic value, and have compared with basic value aobvious
Write increase (p<0.05), skin brightness is obviously improved；A* values have increase trend in embodiment 2 compared with basic value, in embodiment 1 and in fact
Apply example 7 and dramatically increase (p compared with basic value<0.05) it is more ruddy, to show as skin；B* values have drop in each group compared with basic value
It is low, only significantly reduce (p in embodiment 6 and embodiment 7<0.05), de-etiolation positive effect, it is dark yellow to reduce the colour of skin；ITA ° of value exists
Each group is above basic value, and embodiment 1, embodiment 6 and embodiment 7 dramatically increase (p ＜ 0.05), overall skin compared with basic value
Skin is whiter.Can significantly it be carried it can thus be seen that yeast extract, undaria pinnitafida extract solution, palmityl tripeptides -5 are used alone
Skin lightening color, the whitening effect of embodiment 1 are notable.When yeast extract, undaria pinnitafida extraction are applied in combination, individually make better than the two
With；And palmityl tripeptides -5 are added on the basis of the two herein, its whitening effect is more excellent, can significantly improve brilliant white effect
Fruit.
Claims (9)
1. a kind of lightening compositions, it is characterised in that include yeast extract and undaria pinnitafida extract solution.
2. lightening compositions according to claim 1, it is characterised in that the yeast extract accounts for the lightening compositions
The 5% of gross mass, the undaria pinnitafida extract solution account for the 3% of the lightening compositions gross mass.
3. lightening compositions according to claim 2, it is characterised in that also include the percentage by composition gross mass
The following components of meter：Carbomer 0.4%, Phenoxyethanol 0.5%, xanthans 0.6%, mass percent concentration are 1wt%'s
NaOH9.8%.
4. according to the lightening compositions described in any one of claims 1 to 3, it is characterised in that also include palmityl tripeptides -5
Mother liquor.
5. lightening compositions according to claim 4, it is characterised in that the quality percentage of the mother liquor of palmityl tripeptides -5
Specific concentration is 0.1wt%, and the mother liquor of palmityl tripeptides -5 accounts for the 3% of the lightening compositions gross mass.
6. according to the lightening compositions described in any one of claims 1 to 3, it is characterised in that also include the mother liquor of nonapeptide -1 or
The copper mother liquor of tripeptides -1.
7. lightening compositions according to claim 6, it is characterised in that the mass percent concentration of the mother liquor of nonapeptide -1
For 0.01wt%, the mother liquor of nonapeptide -1 accounts for the 8% of the lightening compositions gross mass；The quality hundred of the copper of tripeptides -1 mother liquor
It is 1wt% to divide specific concentration, and the copper of tripeptides -1 mother liquor accounts for the 10% of the lightening compositions gross mass.
8. according to the lightening compositions described in any one of claims 1 to 3, it is characterised in that also include palmityl tripeptides -5
Two or more component in mother liquor, the mother liquor of nonapeptide -1, the copper mother liquor of tripeptides -1, the quality of the mother liquor of palmityl tripeptides -5
Percent concentration is 0.1wt%, and the mass percent concentration of the mother liquor of nonapeptide -1 is 0.01wt%, the copper of tripeptides -1 mother liquor
Mass percent concentration be 1wt%.
9. application of the lightening compositions in skin-lightening cosmetic is prepared described in any one of claim 1~8.
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| CN109157429A (en) * | 2018-09-20 | 2019-01-08 | 深圳市维琪医药研发有限公司 | A kind of active peptides and tyrosinase inhibitor composition for whitening spot-removing |
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| WO2020061184A1 (en) * | 2018-09-18 | 2020-03-26 | Mary Kay Inc. | Plant extracts to reduce the appearance of hyperpigmented skin or to lighten skin |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186400A (en) * | 2017-11-08 | 2018-06-22 | 泉州师范学院 | A kind of moisturizer and preparation method thereof |
| CN107737053A (en) * | 2017-11-30 | 2018-02-27 | 珠海安和生化科技有限公司 | Antiallergy maintenance emulsion and preparation method thereof |
| WO2020061184A1 (en) * | 2018-09-18 | 2020-03-26 | Mary Kay Inc. | Plant extracts to reduce the appearance of hyperpigmented skin or to lighten skin |
| US11883523B2 (en) | 2018-09-18 | 2024-01-30 | Mary Kay Inc. | Plant extracts to reduce the appearance of hyperpigmented skin or to lighten skin |
| CN109157429A (en) * | 2018-09-20 | 2019-01-08 | 深圳市维琪医药研发有限公司 | A kind of active peptides and tyrosinase inhibitor composition for whitening spot-removing |
| CN109157429B (en) * | 2018-09-20 | 2021-07-09 | 深圳市维琪医药研发有限公司 | Active polypeptide and tyrosinase inhibitor composition for whitening and removing freckles |
| CN109481344A (en) * | 2018-12-24 | 2019-03-19 | 无限极（中国）有限公司 | A kind of plant polyose composition and its application |
| CN112998251A (en) * | 2021-01-17 | 2021-06-22 | 贵州大学 | Yeast extract and shiitake mushroom composite hotpot condiment and preparation method thereof |
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| CN107375048B (en) | 2020-07-03 |
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