CN107375005A - A kind of antioxidation and peptide composition and its application for suppressing B16 cell - Google Patents

A kind of antioxidation and peptide composition and its application for suppressing B16 cell Download PDF

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Publication number
CN107375005A
CN107375005A CN201710666550.0A CN201710666550A CN107375005A CN 107375005 A CN107375005 A CN 107375005A CN 201710666550 A CN201710666550 A CN 201710666550A CN 107375005 A CN107375005 A CN 107375005A
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skin
composition
carnosine
peptide
nonapeptide
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CN107375005B (en
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丁文锋
吕庆琴
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Shenzhen Weiqi Technology Co ltd
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Shenzhen Winkey Medical Research Development Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides a kind of peptide composition, includes the combination of one or more of polypeptides with antioxidation and one or more of polypeptides with suppression melanin nucleus formation, wherein each polypeptide moiety mass concentration is 0.0001% 5%.It is dark yellow that said composition is mainly used in skin aging and skin of the improvement/prevention caused by oxidation, so as to reach improvement cutaneous pigmentation, dispel dark yellow and bright color purpose, and prevent the various disease of skin caused by oxidative damage such as lupus erythematosus, polymorphous light eruption etc..

Description

A kind of antioxidation and peptide composition and its application for suppressing B16 cell
Technical field
The invention belongs to medical cosmetic product field, utilizes the polypeptide with antioxidation and the polypeptide of suppression melanin Formula combination is carried out, and use the method for composite reactive polypeptide to prepare various formulations to include creme, Essence, emulsion, freeze-dried powder Deng, for improvement because the skin aging that oxidative stress causes is dark yellow, the oxidative damage of skin is repaired, bright color, and prevent each Disease of skin such as lupus erythematosus, polymorphous light eruption etc. of kind various kinds.
Background technology
Description includes the background information to be come in handy to understanding the present invention below, but is neither to recognize that any provided herein Information is to currently require that the of the invention of protection or the prior art about applying, nor recognizing any specific or implying what is be related to Disclosed prior art.
Skin is the tissue of earliest reflection body aging, has caused numerous scholars aqueous to wrinkle of skin, elasticity and skin The interest of the researchs such as amount.Skin senescence is an extremely complex phenomenon, is related to a series of biological modification, such as skin histology The change of cell, gene and immune system, it is inside and outside various factors (nutrition, environmental pollution, mental element, emotional change, too Sun radiation etc.) coefficient result.According to researcher from different angles and research method is explored to aging, always Many theories on aging are tied, including aging gene theory, telomere shorten theory, free radical theory, immunity function and degenerated Theory etc., wherein oxygen radical theory think closely related with aging by masses.1956, Haman proposed oneself of aging first It is metabolized by base theoretical, it is believed that aging is the result of cell component accumulation property oxidative damage.
Oxidative stress (oxidative stress, OS) refers under destructive stimulus factor, active oxygen (reactive Oxygen species, ROS) produce excessive or dysbolism occurs and energy is removed to it more than endogenous antioxidant defense system During power, ROS increases and participates in the formation of oxidative biological macromolecular in vivo, directly or indirectly oxidation or damage dna, protein and Lipid, the oxidative damage of cell is ultimately resulted in, is embodied in the following aspects:
(1) the oxidative damage of skin inner cell.Keratinocyte (keratinocyte, KC) is ultraviolet B radiation effect Target spot, after being irradiated by ultraviolet, intracellular inflammatory signals transmission mechanism is activated, by expressing or secreting a series of inflammatory cells The factor such as interleukin 1 (IL-1), participate in the immune and inflammatory reaction of body.In addition, ultraviolet can also cause withering for KC Die, may be DNA coup injuries, the formation for inducing ROS and activating cell film surface death receptors.Melanocyte (melanocyte) it is located in epidermis, for maintaining skin tolerance ultraviolet radiation ability significant, is irradiated by ultraviolet Occur afterwards dendron number increase, the morphologic change such as cellular swelling.Fibroblast (fibroblasts) is synthesis collagen and elasticity The main place of fiber, positioned at dermal layer of the skin, oxidative damage but easily occurs.It can be sent out as KC by ultraviolet induction Raw apoptosis.
(2) intracellular all the components, which include protein, can all be aoxidized and be damaged.Oxygen radical increases in skin, can produce Raw a series of reaction:Oxygen radical excessively increases in vivo, can attack cells films, protein, nucleic acid and cause oxidisability to damage Wound, accelerate epiderm skin aging.This is mainly reflected in oxygen radical and main component polyunsaturated fatty acid on cell membrane (PUFA) react, generate lipid peroxidation free radical and MDA (LPO), membrane flow can be made by decomposing the MDA of generation Sexually revise, and lipid peroxidation free radical further accelerates PUFA peroxidating, causes molecular rearrangement, crosslinking between PUFA, carefully After birth mobility reduces, film function of motion obstacle;Oxygen radical can react with base assembles DNA, MDA alkene Aldehyde is broken DNA alkanisation with nucleic acid reaction, so that gene alteration, gene mutation, lose normal function and cause aging;Oxygen is certainly Being attacked by base after fibroblast causes collagenic supersession abnormal, and collage synthesis reduces, and abnormal crosslinking occurs, further influences corium Structure, accelerate skin aging.The impaired protein of oxidation is typically responsible for degraded by protease as main matter, can be due to aging Cause the defense function of protease increasingly to decline, aoxidize impaired protein and accumulate in vivo.In addition, lipofuscin is in the cell Accumulation also hinders the function of protease.It is disorderly that the accumulation damage of cell component can cause histoorgan function further to occur again Disorderly, the further aging of body is caused.
(3) the colour of skin turns yellow, is obscure.The colour of skin turn yellow the reason for have several factors, not only with melanin, ferroheme, carotenoids Element and advanced glycosylation end product AGEs (Advanced Glycation End-products) are relevant, and skin corium Carbonylation of middle collagen and elastin laminin etc. can also affect to colour of skin Huang.It has been reported that albumen in corium It is anti-easily with the aldehyde radical or ketone group of reducing sugar non-glycosylation to occur for amino acid in matter molecule (such as collagenous fibres and elastomer) Should, produce ripe AGEP (AGEs).With advancing age, AGEs progressive increase, AGEs yellowish-brown precipitation Thing is deposited in skin corium, causes yellowing of the skin.The carbonylation of collagen and elastin laminin causes lipofuscin gradually to build up, and also can Cause yellowing of the skin, it is obscure.The tissue change of brown regression caused by carbohydrate and protein, people's custom are referred to as senile plaque expelling.This Outside, AGEs with age, makes collagenous fibres form intermolecular cross-linking, not only reduces the penetrating of connective tissue in skin Property, make the diffusivity of nutriment and waste weaken, organize extensibility and hardness increase, and reduce collagen solubility and be difficult to by Collagen enzyme hydrolysis, causes skin elasticity to decline, and wrinkle is not easy to calm down and constantly deepened, and promotes the aging of skin.Ultraviolet induction Skin aging may be relevant with intradermal AGEs accumulation, have research confirm (Wondrak G T, et al, Photosensitized growth inhibition of cultured human skin cells mechanism and Suppression of oxidative stress from solar irradiation of glycated proteins, 2002,119:489-498), in UV-induced photoaging skin peroxidization, non-glycosylation protein is internal skin This hypothesis of the light sensation thing of skin cell ultraviolet peroxide injury.
Ultraviolet, which has become, causes skin oxidative damage increasingly chief reason.UV-B can be directly thin with skin The DNA of born of the same parents has an effect, and ultraviolet light,long wave then mainly changes core DNA and mitochondria by producing harmful oxygen radical DNA, and matrix metalloproteinase (MMPs) is activated to damage extracellular matrix.MMPs main functions are degraded collagen and bullet The Skin Cell matter matrix components such as property albumen, make the corium thinner layer thickness of skin.Therefore, oxidative damage, which invariably accompanies, declines Always, the problem of and causing skin various, as the colour of skin turn yellow, it is obscure.If oxidative damage further develops, skin can be also produced Disease, such as polymorphous light eruption, it is one of most common photosensitivity disease, its morbidity may be with ROS common in skin (such as The free radicals such as hydroperoxides, singlet oxygen and superoxide anion) it is relevant;Other diseases such as lupus erythematosus, leucoderma, psoriasis Deng, its fall ill may also with ROS induction cell tissue damage it is relevant.
The colour of skin is obscure in addition to oxidative damage factor, also has another factor, i.e., under ultraviolet irradiation, stimulation melanin Cell produces melanin, and the influence of the deposition of melanin to the colour of skin is very big, does not only result in skin color intensification, can also produce spot Point.Also illustrate above, melanocyte has very important meaning to skin tolerance ultraviolet, and caused melanin is absorbable to be had Harmful ultraviolet radiation, so that infringement is reduced to minimum caused by the free radical as ultraviolet induction.But melanocyte It is chronically in oxidative stress, can also produces damage, but its micromechanism of damage is not clear.
Synthesis is described above, and researching and developing some has the anti-oxidant product that effect is generated with suppressing melanin, removes skin certainly By base, delay skin aging, at the same dispel it is dark yellow, lifted skin color, prevent various skin diseases and seem very necessary.
The content of the invention
The present invention is solution above skin problem, there is provided a kind of peptide composition, it is characterised in that include:
(1), have Antioxidation reaction, one or more polypeptides of effect of scavenging radical, selected from carnosine, glutathione, Acetyl carnosine, palmityl carnosine, decarboxylation carnosine;
With
(2), there are the one or more polypeptides for suppressing melanin nucleus formation, selected from (the Nonapeptide-1 of nonapeptide -1 (L- methinyl-L- prolyl-D- phenylalanyl-L- arginyl-D- tryptophanyl-L- phenylalanyl-L- lysyl-L- prolyls- L- valine amides) CAS:158563-45-2), tetrapeptide -27 (Lightenin, Tetrapeptide-27 (Tego- Pep-4Even)), (Decapeptide-12 (Lumixyl of decapeptide -12TM,Envy Medical,Inc.,Westlake Village, USA)), (Hexapeptide-2 (Dermostatyl of hexapeptide -2TMIS));
Each polypeptide moiety mass concentration is 0.0001%-5% more than wherein.
The peptide composition of the present invention can be the two kinds of peptide combinations of carnosine and nonapeptide -1 or two kinds of groups of carnosine and tetrapeptide -27 Close.
The peptide composition of the present invention can also be carnosine and nonapeptide -1, the three kinds of peptide combinations of tetrapeptide -27 or carnosine, acetyl flesh The three kinds of peptide combinations of peptide and nonapeptide -1.
The peptide composition of the present invention can also be carnosine, glutathione and nonapeptide -1, the combination of the four kinds of peptides of tetrapeptide -27 or Carnosine and four kinds of nonapeptide -1, tetrapeptide -27, decapeptide -12 peptide combinations.
Peptide composition of the present invention can add some other compositions with antioxidation, such as ascorbic acid (vitamin C), vitamin E, blumea oil etc..
The composition of the present invention is used for local skin use, can add some NMFs, such as hyaluronic acid, Aloe Vera Gel, sweet Oil etc..Because healthy skin belongs to faintly acid, preferable skin-protection product pH value is 5.0-7.0, therefore its composition is needed to it PH is adjusted, the preferred triethanolamine of pH adjusting agent.
The present composition is to prevent external environment condition pollution and the intrusion of bacterium, can add some preservatives, preferably pungent Glycol;Or antibacterial material, preferably 1,2- hexylene glycols.
Various formulations can be made in the peptide composition of the present invention, as development formulation mainly has Essence, pulvis (glue Capsule, freeze-dried powder), emulsion, creme, gel, facial mask, dressing etc.;Product can be facial mask, moisturizing elite, emulsion, medical dressing Deng.
Peptide composition of the present invention, can local skin use, prevention skin oxidative damage caused by aging, wrinkle, black The colour of skin caused by plain calmness is obscure, blackening, and the skin problem of any combination.
It is dark yellow that peptide composition of the present invention also can be used to improve skin, hyperpigmentation, chloasma, wrinkle, oxidation hair Scorching and any combination skin symptom.
The present invention is advantageous in that:
The present inventor has had many years of experience to polypeptide in the research and probe of dermal region, finds polypeptide to skin nursing Mechanism of action is clear and definite, and has preferable security.Polypeptide is the compound formed by amino acid residue by peptide bond link.According to Different amino acid residue numbers and amino acid residue species on peptide chain, various polypeptides can be formed, but it acts on machine It is formed with institute's difference.Such as suppression approach of the polypeptide to melanin, different polypeptides, its action pathway is probably not quite alike:Nine Peptide -1 (nine amino acid residue compositions) (Pooja Bains, Topical peptides for ageing:potential Role in dermatology, 2016,5:118-125) and hexapeptide -2 (six amino acid residue compositions) is used as MC1-R antagonisms Agent, the activation for suppressing melanocyte reach the effect for suppressing melanin;And decapeptide -12 (ten amino acid residue compositions) is then As the tyrosinase inhibitor in melanocyte, the activity by suppressing tyrosinase reaches the mesh for suppressing melanin generation (Andrea T.kassim, et al, Open-label evaluation of the skin- Brighteningefficacy of a skin-brightening system usingdecapeptide-12,2012,14: 117-121).This several peptide has reached the generation for suppressing melanin, particularly nonapeptide -1 from different action sites and has been also referred to as U.S. White nonapeptide, is widely used in whitening and freckle-removing product by the market.Carnosine is the polypeptide of two amino acid residue compositions, certain Under the conditions of have preferable protective effect to the oxidative damage of skin:Formation to AGE has good inhibiting effect;Generation can be removed Free radical (ROS) and end-product, particularly HNE as caused by ROS inducing lipids peroxidations, the latter can attack NDA Nucleotide base make its mutation;The DNA damage caused by ROS (Reddy VP, et al, Carnosine can effectively be slowed down: A Versatile Antioxidant and Antiglycating Agent, 2005).In recent years, carnosine is also added extensively Into skin nursing products.
At present, the polypeptide products of in the market bright color stress to be to suppress dermal melanin generation or antioxidation, Effect is more single.The mechanism of action that the present invention changes from the microstructure and skin physiology of skin, innovation proposes will The polypeptide (such as carnosine) of antioxidation can be prevented effectively together with polypeptide (such as nonapeptide -1) combination for suppressing melanin generation The aging of skin, and bright color, there is more preferable effect than single use, show preferable cooperative effect.
Brief description of the drawings
Fig. 1 is influence figure of the embodiment 1-4 polypeptides to skin colourity.
Fig. 2 is the influence figure of embodiment 1 and comparative example 1-2 polypeptides to skin colourity.
Embodiment
In order to be better understood from the present invention, invention is described in detail with reference to embodiment and accompanying drawing, but not only It is limited to following embodiment.
The preparation (anti-oxidant Essence) of the peptide composition solution of embodiment 1
By following polypeptide solution prescription, peptide composition of the invention is prepared in the form of Essence.
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, vitamin C, hyalomitome Sour sodium, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 2
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, tetrapeptide -27, vitamin C, hyalomitome Sour sodium, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 3
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, acetyl carnosine, vitamin C, Sodium Hyaluronate, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 4
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, add glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, glutathione, tetrapeptide - 27th, vitamin C, Sodium Hyaluronate, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted with 15% triethanolamine To 5.5 or so.
The preparation (anti-oxidant Essence) of the polypeptide solution of comparative example 1
Except without nonapeptide -1, instead outside deionized water, Essence is prepared by the solution formula of embodiment 1.
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, vitamin C, Sodium Hyaluronate, pungent Glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Comparative example 2
Except without carnosine, instead outside deionized water, Essence is prepared by the solution formula of embodiment 1.
Solution prescription (1 kilogram of Essence):
Collocation method:
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, add glycerine, Aloe Vera Gel, nonapeptide -1, vitamin C, Sodium Hyaluronate, Pungent glycol and 1,2- hexylene glycol, stirs.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 5 improves skin complexion experimental study
Between selecting healthy volunteer's age 18-55, skin is dark yellow on the face, there is obvious color spot, is randomly divided into six groups of groups, Every group of 30 people, wherein four groups use embodiment 1, embodiment 2, embodiment 3, the product of embodiment 4 respectively, two groups of conducts pair in addition According to group, implement the product of 1 and comparative example 2 using contrast respectively, be used continuously 8 weeks.During test, volunteer is immutable Original facial cleansing mode and custom, in addition, unusable other cosmetics in addition to test product.Using product Before, product use 4 weeks and 8 weeks after, skin color test is carried out respectively to both sides cheek, so as to observe product it is dark yellow to skin with The situation that color spot improves.
Colour difference meter Chromameter (CR-400, Konica Minolta, Japan) the measurement colour of skin is used in test.This instrument Device represents the change of the colour of skin using colorimeter system (Lab colorimeter systems) as defined in International Commission on Illumination (CIE).Wherein:
L* is brightness, and a* is red green degree, and b* is blue yellow chromaticity, and the overall merit of three can reflect the change of skin colourity Change;ITA ° of value is skin subjects type angle, is the numerical value of the sign skin lightness related to L* and b*.The ITA ° of bigger skin of value It is brighter, conversely, skin is more obscure.
The test zone of skin color test (Chromameter) is left and right sides cheek, and each test section is tested 3 times and taken Average value.As a result attached Fig. 1 and 2 is seen.
Come out from the experimental results, polypeptide can improve the colourity of skin, and the time used is more long, and effect is more obvious;Flesh Using effect of the effect better than single carnosine or single nonapeptide -1 is applied in combination with nonapeptide -1 in peptide, and different types of peptide is inferred in side It is applied in combination, can be concured from different target spots, so as to reach improvement pigmentation, bright color and is dispelled dark at nti-freckle Yellow effect, has good synergy.
Above content is to combine the further details of explanation that specific preferred embodiment is done to the present invention, but not Represent that the specific implementation of the present invention is limited to these explanations.For those skilled in the art, On the premise of not departing from present inventive concept, some simple deductions can also be made or replaced.It the foregoing is only the present invention's Specific embodiment, it is not intended to limit the invention, any modification for being made within the spirit and principles of the invention, equally replaces Change and improve, should be included in the scope of the protection.

Claims (10)

1. a kind of peptide composition, it is characterised in that include:
(1), there are Antioxidation reaction, one or more polypeptides of effect of scavenging radical, selected from carnosine, glutathione, acetyl Carnosine, palmityl carnosine and decarboxylation carnosine;
(2), have suppress melanin nucleus formation one or more polypeptides, selected from nonapeptide -1, tetrapeptide -27, decapeptide -12 and Hexapeptide -2;The mass percent concentration of each polypeptide moiety is 0.0001%-5% more than wherein.
2. the composition as described in claim 1, it is characterised in that the peptide composition is selected from following combination:Carnosine and Two kinds of combinations of the two kinds of peptide combinations of nonapeptide -1 or carnosine and tetrapeptide -27;Carnosine and nonapeptide -1, the three kinds of peptide combinations of tetrapeptide -27 or flesh Three kinds of peptide, acetyl carnosine and nonapeptide -1 peptide combinations;Carnosine, glutathione and nonapeptide -1, the four kinds of peptide combinations of tetrapeptide -27 or carnosine With four kinds of nonapeptide -1, tetrapeptide -27, decapeptide -12 peptide combinations.
3. as the composition described in claim 1-2, it is characterised in that the peptide composition also add selected from ascorbic acid, Vitamin E, blumea oil other there is the composition of antioxidation, each ingredient percent concentration is 0.001%-5%.
4. as the composition described in claim 1-2 any claims, it is characterised in that the peptide composition is also added with More alcohols preservatives;And/or anti-microbial type reagent;The mass percent concentration scope of these compositions is 0.001%-5%.
5. as the composition described in claim 1-2 any claims, it is characterised in that more alcohols preservatives are pungent sweet Alcohol;Anti-microbial type reagent is 1,2- hexylene glycols.
6. the composition as described in claim 1-2 any claims, it is characterised in that the peptide composition is also added with choosing From the NMF of glycerine, Aloe Vera Gel and hyaluronic acid, its mass percent concentration scope is 0.001%-5%.
7. as the composition described in claim 1-2 any claims, it is characterised in that the peptide composition also includes choosing From the pH adjusting agent of triethanolamine, sodium acid carbonate and saleratus;It is preferred that triethanolamine;Composition pH scope be 3.0~ 8.0, preferably 5.0~7.0.
8. as the composition described in claim 1-2 any claims, it is characterised in that the combination dosage form is elite Liquid, pulvis, capsule, freeze-dried powder, emulsion, creme, gel, facial mask or dressing.
9. the application of the composition as described in claim 1-8 any claim, its as selected from facial mask, moisturizing elite, Emulsion, eye cream, the external skin product of medical dressing.
10. the application of composition according to claim 9, its be used for aging caused by preventing skin oxidative damage, wrinkle, The colour of skin caused by melanin pigmentation is obscure, the skin problem of blackening and any combination;Or skin is dark yellow, pigmentation for improving Excessively, the skin symptom of color spot, wrinkle, oxidation inflammation and any combination.
CN201710666550.0A 2017-08-07 2017-08-07 Polypeptide composition with antioxidation and melanin synthesis inhibition functions and application thereof Active CN107375005B (en)

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CN108703894A (en) * 2018-08-21 2018-10-26 广东博然堂生物科技有限公司 It is a kind of that there is whitening, anti-ageing complex polypeptide composition and preparation method thereof
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CN109180785A (en) * 2018-11-08 2019-01-11 中国科学院上海应用物理研究所 A kind of whitening reducing polypeptide and its application with inhibition melanin production and ultraviolet injury resistant effect
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CN112641652A (en) * 2020-12-30 2021-04-13 宇肽生物(东莞)有限公司 Sub-disposable polypeptide composition and preparation method thereof
CN114042009A (en) * 2021-12-01 2022-02-15 宇肽生物(东莞)有限公司 Polypeptide composition with antioxidation and repair effects and application thereof
CN114306113A (en) * 2022-01-20 2022-04-12 深圳市萱嘉生物科技有限公司 Supramolecular carnosine, composition containing supramolecular carnosine, and preparation method and application of supramolecular carnosine
CN115025001A (en) * 2022-05-31 2022-09-09 陕西慧康生物科技有限责任公司 Peptide composition with multiple whitening and freckle removing effects and application thereof
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CN108066245A (en) * 2018-02-09 2018-05-25 广州御延堂生物科技有限公司 A kind of skin whitening, moisturizing stoste and preparation method thereof
CN108324589A (en) * 2018-05-11 2018-07-27 广东省中药研究所 A kind of composition for whitening skin and preparation method thereof
CN108703894A (en) * 2018-08-21 2018-10-26 广东博然堂生物科技有限公司 It is a kind of that there is whitening, anti-ageing complex polypeptide composition and preparation method thereof
CN109157429B (en) * 2018-09-20 2021-07-09 深圳市维琪医药研发有限公司 Active polypeptide and tyrosinase inhibitor composition for whitening and removing freckles
CN109157429A (en) * 2018-09-20 2019-01-08 深圳市维琪医药研发有限公司 A kind of active peptides and tyrosinase inhibitor composition for whitening spot-removing
CN109010116A (en) * 2018-10-23 2018-12-18 深圳市吉爱丝迪生物科技有限公司 A kind of whitening spot-eliminating composition and its preparation method and application
CN109180785A (en) * 2018-11-08 2019-01-11 中国科学院上海应用物理研究所 A kind of whitening reducing polypeptide and its application with inhibition melanin production and ultraviolet injury resistant effect
RU2809007C2 (en) * 2019-01-19 2023-12-05 Эскейп Терапьютикс, Инк. Tyrosine inhibitors with immunosuppressive activity in human neonatal keratinocyte progenous cells
CN109432395A (en) * 2019-01-22 2019-03-08 宇肽生物(东莞)有限公司 A kind of whitening spot-removing active peptides
CN109893463A (en) * 2019-04-24 2019-06-18 深圳市健翔生物制药有限公司 A kind of whitening peptide composition and its preparation and use
CN110115694A (en) * 2019-05-23 2019-08-13 中山市粤美医疗生物科技有限公司 A kind of Essence with whitening, effect of dispelling spots
CN111494224A (en) * 2020-06-16 2020-08-07 四川天晟制药有限公司 Whitening and spot-fading composition, skin care product and preparation method thereof
CN112386520A (en) * 2020-11-24 2021-02-23 西安润玉医疗科技有限公司 Mesoderm injection composition for comprehensively improving skin color and preparation method thereof
CN112641652A (en) * 2020-12-30 2021-04-13 宇肽生物(东莞)有限公司 Sub-disposable polypeptide composition and preparation method thereof
CN114042009A (en) * 2021-12-01 2022-02-15 宇肽生物(东莞)有限公司 Polypeptide composition with antioxidation and repair effects and application thereof
CN114306113A (en) * 2022-01-20 2022-04-12 深圳市萱嘉生物科技有限公司 Supramolecular carnosine, composition containing supramolecular carnosine, and preparation method and application of supramolecular carnosine
CN114306113B (en) * 2022-01-20 2024-03-26 深圳杉海创新技术有限公司 Supermolecular carnosine, composition containing supermolecular carnosine, and preparation methods and applications thereof
CN115025001A (en) * 2022-05-31 2022-09-09 陕西慧康生物科技有限责任公司 Peptide composition with multiple whitening and freckle removing effects and application thereof
CN115025001B (en) * 2022-05-31 2023-12-05 陕西慧康生物科技有限责任公司 Peptide composition with multiple whitening and freckle removing effects and application thereof

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