CN107375005A - A kind of antioxidation and peptide composition and its application for suppressing B16 cell - Google Patents
A kind of antioxidation and peptide composition and its application for suppressing B16 cell Download PDFInfo
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- CN107375005A CN107375005A CN201710666550.0A CN201710666550A CN107375005A CN 107375005 A CN107375005 A CN 107375005A CN 201710666550 A CN201710666550 A CN 201710666550A CN 107375005 A CN107375005 A CN 107375005A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The present invention provides a kind of peptide composition, includes the combination of one or more of polypeptides with antioxidation and one or more of polypeptides with suppression melanin nucleus formation, wherein each polypeptide moiety mass concentration is 0.0001% 5%.It is dark yellow that said composition is mainly used in skin aging and skin of the improvement/prevention caused by oxidation, so as to reach improvement cutaneous pigmentation, dispel dark yellow and bright color purpose, and prevent the various disease of skin caused by oxidative damage such as lupus erythematosus, polymorphous light eruption etc..
Description
Technical field
The invention belongs to medical cosmetic product field, utilizes the polypeptide with antioxidation and the polypeptide of suppression melanin
Formula combination is carried out, and use the method for composite reactive polypeptide to prepare various formulations to include creme, Essence, emulsion, freeze-dried powder
Deng, for improvement because the skin aging that oxidative stress causes is dark yellow, the oxidative damage of skin is repaired, bright color, and prevent each
Disease of skin such as lupus erythematosus, polymorphous light eruption etc. of kind various kinds.
Background technology
Description includes the background information to be come in handy to understanding the present invention below, but is neither to recognize that any provided herein
Information is to currently require that the of the invention of protection or the prior art about applying, nor recognizing any specific or implying what is be related to
Disclosed prior art.
Skin is the tissue of earliest reflection body aging, has caused numerous scholars aqueous to wrinkle of skin, elasticity and skin
The interest of the researchs such as amount.Skin senescence is an extremely complex phenomenon, is related to a series of biological modification, such as skin histology
The change of cell, gene and immune system, it is inside and outside various factors (nutrition, environmental pollution, mental element, emotional change, too
Sun radiation etc.) coefficient result.According to researcher from different angles and research method is explored to aging, always
Many theories on aging are tied, including aging gene theory, telomere shorten theory, free radical theory, immunity function and degenerated
Theory etc., wherein oxygen radical theory think closely related with aging by masses.1956, Haman proposed oneself of aging first
It is metabolized by base theoretical, it is believed that aging is the result of cell component accumulation property oxidative damage.
Oxidative stress (oxidative stress, OS) refers under destructive stimulus factor, active oxygen (reactive
Oxygen species, ROS) produce excessive or dysbolism occurs and energy is removed to it more than endogenous antioxidant defense system
During power, ROS increases and participates in the formation of oxidative biological macromolecular in vivo, directly or indirectly oxidation or damage dna, protein and
Lipid, the oxidative damage of cell is ultimately resulted in, is embodied in the following aspects：
(1) the oxidative damage of skin inner cell.Keratinocyte (keratinocyte, KC) is ultraviolet B radiation effect
Target spot, after being irradiated by ultraviolet, intracellular inflammatory signals transmission mechanism is activated, by expressing or secreting a series of inflammatory cells
The factor such as interleukin 1 (IL-1), participate in the immune and inflammatory reaction of body.In addition, ultraviolet can also cause withering for KC
Die, may be DNA coup injuries, the formation for inducing ROS and activating cell film surface death receptors.Melanocyte
(melanocyte) it is located in epidermis, for maintaining skin tolerance ultraviolet radiation ability significant, is irradiated by ultraviolet
Occur afterwards dendron number increase, the morphologic change such as cellular swelling.Fibroblast (fibroblasts) is synthesis collagen and elasticity
The main place of fiber, positioned at dermal layer of the skin, oxidative damage but easily occurs.It can be sent out as KC by ultraviolet induction
Raw apoptosis.
(2) intracellular all the components, which include protein, can all be aoxidized and be damaged.Oxygen radical increases in skin, can produce
Raw a series of reaction：Oxygen radical excessively increases in vivo, can attack cells films, protein, nucleic acid and cause oxidisability to damage
Wound, accelerate epiderm skin aging.This is mainly reflected in oxygen radical and main component polyunsaturated fatty acid on cell membrane
(PUFA) react, generate lipid peroxidation free radical and MDA (LPO), membrane flow can be made by decomposing the MDA of generation
Sexually revise, and lipid peroxidation free radical further accelerates PUFA peroxidating, causes molecular rearrangement, crosslinking between PUFA, carefully
After birth mobility reduces, film function of motion obstacle；Oxygen radical can react with base assembles DNA, MDA alkene
Aldehyde is broken DNA alkanisation with nucleic acid reaction, so that gene alteration, gene mutation, lose normal function and cause aging；Oxygen is certainly
Being attacked by base after fibroblast causes collagenic supersession abnormal, and collage synthesis reduces, and abnormal crosslinking occurs, further influences corium
Structure, accelerate skin aging.The impaired protein of oxidation is typically responsible for degraded by protease as main matter, can be due to aging
Cause the defense function of protease increasingly to decline, aoxidize impaired protein and accumulate in vivo.In addition, lipofuscin is in the cell
Accumulation also hinders the function of protease.It is disorderly that the accumulation damage of cell component can cause histoorgan function further to occur again
Disorderly, the further aging of body is caused.
(3) the colour of skin turns yellow, is obscure.The colour of skin turn yellow the reason for have several factors, not only with melanin, ferroheme, carotenoids
Element and advanced glycosylation end product AGEs (Advanced Glycation End-products) are relevant, and skin corium
Carbonylation of middle collagen and elastin laminin etc. can also affect to colour of skin Huang.It has been reported that albumen in corium
It is anti-easily with the aldehyde radical or ketone group of reducing sugar non-glycosylation to occur for amino acid in matter molecule (such as collagenous fibres and elastomer)
Should, produce ripe AGEP (AGEs).With advancing age, AGEs progressive increase, AGEs yellowish-brown precipitation
Thing is deposited in skin corium, causes yellowing of the skin.The carbonylation of collagen and elastin laminin causes lipofuscin gradually to build up, and also can
Cause yellowing of the skin, it is obscure.The tissue change of brown regression caused by carbohydrate and protein, people's custom are referred to as senile plaque expelling.This
Outside, AGEs with age, makes collagenous fibres form intermolecular cross-linking, not only reduces the penetrating of connective tissue in skin
Property, make the diffusivity of nutriment and waste weaken, organize extensibility and hardness increase, and reduce collagen solubility and be difficult to by
Collagen enzyme hydrolysis, causes skin elasticity to decline, and wrinkle is not easy to calm down and constantly deepened, and promotes the aging of skin.Ultraviolet induction
Skin aging may be relevant with intradermal AGEs accumulation, have research confirm (Wondrak G T, et al,
Photosensitized growth inhibition of cultured human skin cells mechanism and
Suppression of oxidative stress from solar irradiation of glycated proteins,
2002,119：489-498), in UV-induced photoaging skin peroxidization, non-glycosylation protein is internal skin
This hypothesis of the light sensation thing of skin cell ultraviolet peroxide injury.
Ultraviolet, which has become, causes skin oxidative damage increasingly chief reason.UV-B can be directly thin with skin
The DNA of born of the same parents has an effect, and ultraviolet light,long wave then mainly changes core DNA and mitochondria by producing harmful oxygen radical
DNA, and matrix metalloproteinase (MMPs) is activated to damage extracellular matrix.MMPs main functions are degraded collagen and bullet
The Skin Cell matter matrix components such as property albumen, make the corium thinner layer thickness of skin.Therefore, oxidative damage, which invariably accompanies, declines
Always, the problem of and causing skin various, as the colour of skin turn yellow, it is obscure.If oxidative damage further develops, skin can be also produced
Disease, such as polymorphous light eruption, it is one of most common photosensitivity disease, its morbidity may be with ROS common in skin (such as
The free radicals such as hydroperoxides, singlet oxygen and superoxide anion) it is relevant；Other diseases such as lupus erythematosus, leucoderma, psoriasis
Deng, its fall ill may also with ROS induction cell tissue damage it is relevant.
The colour of skin is obscure in addition to oxidative damage factor, also has another factor, i.e., under ultraviolet irradiation, stimulation melanin
Cell produces melanin, and the influence of the deposition of melanin to the colour of skin is very big, does not only result in skin color intensification, can also produce spot
Point.Also illustrate above, melanocyte has very important meaning to skin tolerance ultraviolet, and caused melanin is absorbable to be had
Harmful ultraviolet radiation, so that infringement is reduced to minimum caused by the free radical as ultraviolet induction.But melanocyte
It is chronically in oxidative stress, can also produces damage, but its micromechanism of damage is not clear.
Synthesis is described above, and researching and developing some has the anti-oxidant product that effect is generated with suppressing melanin, removes skin certainly
By base, delay skin aging, at the same dispel it is dark yellow, lifted skin color, prevent various skin diseases and seem very necessary.
The content of the invention
The present invention is solution above skin problem, there is provided a kind of peptide composition, it is characterised in that include：
(1), have Antioxidation reaction, one or more polypeptides of effect of scavenging radical, selected from carnosine, glutathione,
Acetyl carnosine, palmityl carnosine, decarboxylation carnosine；
With
(2), there are the one or more polypeptides for suppressing melanin nucleus formation, selected from (the Nonapeptide-1 of nonapeptide -1
(L- methinyl-L- prolyl-D- phenylalanyl-L- arginyl-D- tryptophanyl-L- phenylalanyl-L- lysyl-L- prolyls-
L- valine amides) CAS:158563-45-2), tetrapeptide -27 (Lightenin, Tetrapeptide-27 (Tego-
Pep-4Even)), (Decapeptide-12 (Lumixyl of decapeptide -12TM,Envy Medical,Inc.,Westlake
Village, USA)), (Hexapeptide-2 (Dermostatyl of hexapeptide -2TMIS))；
Each polypeptide moiety mass concentration is 0.0001%-5% more than wherein.
The peptide composition of the present invention can be the two kinds of peptide combinations of carnosine and nonapeptide -1 or two kinds of groups of carnosine and tetrapeptide -27
Close.
The peptide composition of the present invention can also be carnosine and nonapeptide -1, the three kinds of peptide combinations of tetrapeptide -27 or carnosine, acetyl flesh
The three kinds of peptide combinations of peptide and nonapeptide -1.
The peptide composition of the present invention can also be carnosine, glutathione and nonapeptide -1, the combination of the four kinds of peptides of tetrapeptide -27 or
Carnosine and four kinds of nonapeptide -1, tetrapeptide -27, decapeptide -12 peptide combinations.
Peptide composition of the present invention can add some other compositions with antioxidation, such as ascorbic acid (vitamin
C), vitamin E, blumea oil etc..
The composition of the present invention is used for local skin use, can add some NMFs, such as hyaluronic acid, Aloe Vera Gel, sweet
Oil etc..Because healthy skin belongs to faintly acid, preferable skin-protection product pH value is 5.0-7.0, therefore its composition is needed to it
PH is adjusted, the preferred triethanolamine of pH adjusting agent.
The present composition is to prevent external environment condition pollution and the intrusion of bacterium, can add some preservatives, preferably pungent
Glycol；Or antibacterial material, preferably 1,2- hexylene glycols.
Various formulations can be made in the peptide composition of the present invention, as development formulation mainly has Essence, pulvis (glue
Capsule, freeze-dried powder), emulsion, creme, gel, facial mask, dressing etc.；Product can be facial mask, moisturizing elite, emulsion, medical dressing
Deng.
Peptide composition of the present invention, can local skin use, prevention skin oxidative damage caused by aging, wrinkle, black
The colour of skin caused by plain calmness is obscure, blackening, and the skin problem of any combination.
It is dark yellow that peptide composition of the present invention also can be used to improve skin, hyperpigmentation, chloasma, wrinkle, oxidation hair
Scorching and any combination skin symptom.
The present invention is advantageous in that：
The present inventor has had many years of experience to polypeptide in the research and probe of dermal region, finds polypeptide to skin nursing
Mechanism of action is clear and definite, and has preferable security.Polypeptide is the compound formed by amino acid residue by peptide bond link.According to
Different amino acid residue numbers and amino acid residue species on peptide chain, various polypeptides can be formed, but it acts on machine
It is formed with institute's difference.Such as suppression approach of the polypeptide to melanin, different polypeptides, its action pathway is probably not quite alike：Nine
Peptide -1 (nine amino acid residue compositions) (Pooja Bains, Topical peptides for ageing：potential
Role in dermatology, 2016,5:118-125) and hexapeptide -2 (six amino acid residue compositions) is used as MC1-R antagonisms
Agent, the activation for suppressing melanocyte reach the effect for suppressing melanin；And decapeptide -12 (ten amino acid residue compositions) is then
As the tyrosinase inhibitor in melanocyte, the activity by suppressing tyrosinase reaches the mesh for suppressing melanin generation
(Andrea T.kassim, et al, Open-label evaluation of the skin-
Brighteningefficacy of a skin-brightening system usingdecapeptide-12,2012,14:
117-121).This several peptide has reached the generation for suppressing melanin, particularly nonapeptide -1 from different action sites and has been also referred to as U.S.
White nonapeptide, is widely used in whitening and freckle-removing product by the market.Carnosine is the polypeptide of two amino acid residue compositions, certain
Under the conditions of have preferable protective effect to the oxidative damage of skin：Formation to AGE has good inhibiting effect；Generation can be removed
Free radical (ROS) and end-product, particularly HNE as caused by ROS inducing lipids peroxidations, the latter can attack NDA
Nucleotide base make its mutation；The DNA damage caused by ROS (Reddy VP, et al, Carnosine can effectively be slowed down:
A Versatile Antioxidant and Antiglycating Agent, 2005).In recent years, carnosine is also added extensively
Into skin nursing products.
At present, the polypeptide products of in the market bright color stress to be to suppress dermal melanin generation or antioxidation,
Effect is more single.The mechanism of action that the present invention changes from the microstructure and skin physiology of skin, innovation proposes will
The polypeptide (such as carnosine) of antioxidation can be prevented effectively together with polypeptide (such as nonapeptide -1) combination for suppressing melanin generation
The aging of skin, and bright color, there is more preferable effect than single use, show preferable cooperative effect.
Brief description of the drawings
Fig. 1 is influence figure of the embodiment 1-4 polypeptides to skin colourity.
Fig. 2 is the influence figure of embodiment 1 and comparative example 1-2 polypeptides to skin colourity.
Embodiment
In order to be better understood from the present invention, invention is described in detail with reference to embodiment and accompanying drawing, but not only
It is limited to following embodiment.
The preparation (anti-oxidant Essence) of the peptide composition solution of embodiment 1
By following polypeptide solution prescription, peptide composition of the invention is prepared in the form of Essence.
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, vitamin C, hyalomitome
Sour sodium, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 2
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, tetrapeptide -27, vitamin C, hyalomitome
Sour sodium, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 3
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, acetyl carnosine, vitamin
C, Sodium Hyaluronate, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 4
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, add glycerine, Aloe Vera Gel, carnosine, nonapeptide -1, glutathione, tetrapeptide -
27th, vitamin C, Sodium Hyaluronate, pungent glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted with 15% triethanolamine
To 5.5 or so.
The preparation (anti-oxidant Essence) of the polypeptide solution of comparative example 1
Except without nonapeptide -1, instead outside deionized water, Essence is prepared by the solution formula of embodiment 1.
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, adds glycerine, Aloe Vera Gel, carnosine, vitamin C, Sodium Hyaluronate, pungent
Glycol and 1,2- hexylene glycol, stir.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Comparative example 2
Except without carnosine, instead outside deionized water, Essence is prepared by the solution formula of embodiment 1.
Solution prescription (1 kilogram of Essence)：
Collocation method：
The Sodium Hyaluronate of formula ratio is added to the water, stirring makes to be well mixed, and is then heated to 80~85 DEG C, insulation
Stirring makes it be uniformly dispersed.Temperature drops to less than 40 DEG C, add glycerine, Aloe Vera Gel, nonapeptide -1, vitamin C, Sodium Hyaluronate,
Pungent glycol and 1,2- hexylene glycol, stirs.The pH value of solution is adjusted to 5.5 or so with 15% triethanolamine.
Embodiment 5 improves skin complexion experimental study
Between selecting healthy volunteer's age 18-55, skin is dark yellow on the face, there is obvious color spot, is randomly divided into six groups of groups,
Every group of 30 people, wherein four groups use embodiment 1, embodiment 2, embodiment 3, the product of embodiment 4 respectively, two groups of conducts pair in addition
According to group, implement the product of 1 and comparative example 2 using contrast respectively, be used continuously 8 weeks.During test, volunteer is immutable
Original facial cleansing mode and custom, in addition, unusable other cosmetics in addition to test product.Using product
Before, product use 4 weeks and 8 weeks after, skin color test is carried out respectively to both sides cheek, so as to observe product it is dark yellow to skin with
The situation that color spot improves.
Colour difference meter Chromameter (CR-400, Konica Minolta, Japan) the measurement colour of skin is used in test.This instrument
Device represents the change of the colour of skin using colorimeter system (Lab colorimeter systems) as defined in International Commission on Illumination (CIE).Wherein：
L* is brightness, and a* is red green degree, and b* is blue yellow chromaticity, and the overall merit of three can reflect the change of skin colourity
Change；ITA ° of value is skin subjects type angle, is the numerical value of the sign skin lightness related to L* and b*.The ITA ° of bigger skin of value
It is brighter, conversely, skin is more obscure.
The test zone of skin color test (Chromameter) is left and right sides cheek, and each test section is tested 3 times and taken
Average value.As a result attached Fig. 1 and 2 is seen.
Come out from the experimental results, polypeptide can improve the colourity of skin, and the time used is more long, and effect is more obvious；Flesh
Using effect of the effect better than single carnosine or single nonapeptide -1 is applied in combination with nonapeptide -1 in peptide, and different types of peptide is inferred in side
It is applied in combination, can be concured from different target spots, so as to reach improvement pigmentation, bright color and is dispelled dark at nti-freckle
Yellow effect, has good synergy.
Above content is to combine the further details of explanation that specific preferred embodiment is done to the present invention, but not
Represent that the specific implementation of the present invention is limited to these explanations.For those skilled in the art,
On the premise of not departing from present inventive concept, some simple deductions can also be made or replaced.It the foregoing is only the present invention's
Specific embodiment, it is not intended to limit the invention, any modification for being made within the spirit and principles of the invention, equally replaces
Change and improve, should be included in the scope of the protection.
Claims (10)
1. a kind of peptide composition, it is characterised in that include：
(1), there are Antioxidation reaction, one or more polypeptides of effect of scavenging radical, selected from carnosine, glutathione, acetyl
Carnosine, palmityl carnosine and decarboxylation carnosine；
(2), have suppress melanin nucleus formation one or more polypeptides, selected from nonapeptide -1, tetrapeptide -27, decapeptide -12 and
Hexapeptide -2；The mass percent concentration of each polypeptide moiety is 0.0001%-5% more than wherein.
2. the composition as described in claim 1, it is characterised in that the peptide composition is selected from following combination：Carnosine and
Two kinds of combinations of the two kinds of peptide combinations of nonapeptide -1 or carnosine and tetrapeptide -27；Carnosine and nonapeptide -1, the three kinds of peptide combinations of tetrapeptide -27 or flesh
Three kinds of peptide, acetyl carnosine and nonapeptide -1 peptide combinations；Carnosine, glutathione and nonapeptide -1, the four kinds of peptide combinations of tetrapeptide -27 or carnosine
With four kinds of nonapeptide -1, tetrapeptide -27, decapeptide -12 peptide combinations.
3. as the composition described in claim 1-2, it is characterised in that the peptide composition also add selected from ascorbic acid,
Vitamin E, blumea oil other there is the composition of antioxidation, each ingredient percent concentration is 0.001%-5%.
4. as the composition described in claim 1-2 any claims, it is characterised in that the peptide composition is also added with
More alcohols preservatives；And/or anti-microbial type reagent；The mass percent concentration scope of these compositions is 0.001%-5%.
5. as the composition described in claim 1-2 any claims, it is characterised in that more alcohols preservatives are pungent sweet
Alcohol；Anti-microbial type reagent is 1,2- hexylene glycols.
6. the composition as described in claim 1-2 any claims, it is characterised in that the peptide composition is also added with choosing
From the NMF of glycerine, Aloe Vera Gel and hyaluronic acid, its mass percent concentration scope is 0.001%-5%.
7. as the composition described in claim 1-2 any claims, it is characterised in that the peptide composition also includes choosing
From the pH adjusting agent of triethanolamine, sodium acid carbonate and saleratus；It is preferred that triethanolamine；Composition pH scope be 3.0~
8.0, preferably 5.0~7.0.
8. as the composition described in claim 1-2 any claims, it is characterised in that the combination dosage form is elite
Liquid, pulvis, capsule, freeze-dried powder, emulsion, creme, gel, facial mask or dressing.
9. the application of the composition as described in claim 1-8 any claim, its as selected from facial mask, moisturizing elite,
Emulsion, eye cream, the external skin product of medical dressing.
10. the application of composition according to claim 9, its be used for aging caused by preventing skin oxidative damage, wrinkle,
The colour of skin caused by melanin pigmentation is obscure, the skin problem of blackening and any combination；Or skin is dark yellow, pigmentation for improving
Excessively, the skin symptom of color spot, wrinkle, oxidation inflammation and any combination.
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| CN109157429B (en) * | 2018-09-20 | 2021-07-09 | 深圳市维琪医药研发有限公司 | Active polypeptide and tyrosinase inhibitor composition for whitening and removing freckles |
| CN109157429A (en) * | 2018-09-20 | 2019-01-08 | 深圳市维琪医药研发有限公司 | A kind of active peptides and tyrosinase inhibitor composition for whitening spot-removing |
| CN109010116A (en) * | 2018-10-23 | 2018-12-18 | 深圳市吉爱丝迪生物科技有限公司 | A kind of whitening spot-eliminating composition and its preparation method and application |
| CN109180785A (en) * | 2018-11-08 | 2019-01-11 | 中国科学院上海应用物理研究所 | A kind of whitening reducing polypeptide and its application with inhibition melanin production and ultraviolet injury resistant effect |
| RU2809007C2 (en) * | 2019-01-19 | 2023-12-05 | Эскейп Терапьютикс, Инк. | Tyrosine inhibitors with immunosuppressive activity in human neonatal keratinocyte progenous cells |
| CN109432395A (en) * | 2019-01-22 | 2019-03-08 | 宇肽生物(东莞)有限公司 | A kind of whitening spot-removing active peptides |
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| CN110115694A (en) * | 2019-05-23 | 2019-08-13 | 中山市粤美医疗生物科技有限公司 | A kind of Essence with whitening, effect of dispelling spots |
| CN111494224A (en) * | 2020-06-16 | 2020-08-07 | 四川天晟制药有限公司 | Whitening and spot-fading composition, skin care product and preparation method thereof |
| CN112386520A (en) * | 2020-11-24 | 2021-02-23 | 西安润玉医疗科技有限公司 | Mesoderm injection composition for comprehensively improving skin color and preparation method thereof |
| CN112641652A (en) * | 2020-12-30 | 2021-04-13 | 宇肽生物(东莞)有限公司 | Sub-disposable polypeptide composition and preparation method thereof |
| CN114042009A (en) * | 2021-12-01 | 2022-02-15 | 宇肽生物(东莞)有限公司 | Polypeptide composition with antioxidation and repair effects and application thereof |
| CN114306113A (en) * | 2022-01-20 | 2022-04-12 | 深圳市萱嘉生物科技有限公司 | Supramolecular carnosine, composition containing supramolecular carnosine, and preparation method and application of supramolecular carnosine |
| CN114306113B (en) * | 2022-01-20 | 2024-03-26 | 深圳杉海创新技术有限公司 | Supermolecular carnosine, composition containing supermolecular carnosine, and preparation methods and applications thereof |
| CN115025001A (en) * | 2022-05-31 | 2022-09-09 | 陕西慧康生物科技有限责任公司 | Peptide composition with multiple whitening and freckle removing effects and application thereof |
| CN115025001B (en) * | 2022-05-31 | 2023-12-05 | 陕西慧康生物科技有限责任公司 | Peptide composition with multiple whitening and freckle removing effects and application thereof |
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