CN106258368A - A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon - Google Patents

A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon Download PDF

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Publication number
CN106258368A
CN106258368A CN201610658292.7A CN201610658292A CN106258368A CN 106258368 A CN106258368 A CN 106258368A CN 201610658292 A CN201610658292 A CN 201610658292A CN 106258368 A CN106258368 A CN 106258368A
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China
Prior art keywords
stolon
seedling
fructus fragariae
fragariae ananssae
root
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CN201610658292.7A
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Inventor
宁志怨
董玲
廖华俊
李卫文
江芹
闫冲冲
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Institute of Gardening of Anhui Academy Agricultural Sciences
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Institute of Gardening of Anhui Academy Agricultural Sciences
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Priority to CN201610658292.7A priority Critical patent/CN106258368A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses the new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon, the method includes following seedling step: the induction of Fructus Fragariae Ananssae stolon, the shearing of stolon, classification and pretreatment, the induction of aerial root, transplanting and seedling.The method may be used for the efficient sprouting and rooting of overhead stolon of the Fructus Fragariae Ananssae improved Varieties such as beauty, a chapter Ji.Instant invention overcomes the stolon of early origin to take root more difficult deficiency, produce aerial root by induction stolon in early days, then to substrate and short-term dark culturing, make the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon by transplanting.The method planting percent is typically 85~more than 89%, and has the features such as simple, convenient and seedling speed is fast.The inventive method induction stolon produces that time simple compared with cuttage, convenient and required time of aerial root is shorter, concentrates, and it is high and more stable that different time, different batches use the method to produce filial generation stolon Seedling quality.

Description

A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon
Technical field
The invention belongs to garden crop seedling-raising technique field, be specifically related to the new of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon Method.
Background technology
Fructus Fragariae Ananssae is Rosaceae Fragaria herbaceos perennial, has strong adaptability, with short production cycle, instant effect, income High feature, and containing rich in protein and vitamin, it is described as " fruit queen ", deeply by the green grass or young crops of producers and consumers Look at.Utilize the facility cultivation such as heliogreenhouse, hot house and coordinate corresponding technical measures, Fructus Fragariae Ananssae can be made to list in advance, becoming For the fancy fruit of the main festive occasion such as Christmas Day, New Year's Day, the Spring Festival, economic benefit is the most considerable.
Although Fructus Fragariae Ananssae breeding and seedling nursing with equipment technology achieves the development of brilliance, but for various reasons, most plantation families are still adopted With the method for culturing seedlings of relatively simple, extensive open country stolon breeding, use by oneself from numerous self-fertile for a long time, cause strawberry seedling breeding Have that virosis frequently occurs, variety deterioration is serious, anthrax infection is serious, breeding coefficient is low, rich owe irregular, soil-borne disease is tight Weight, thus increase the risk of production.
The problems referred to above, while increasing production cost and strengthening production risk, the most seriously govern Fructus Fragariae Ananssae improved seeds Rapid Popularization.The implant mass of some improved seeds such as beauty, a chapter Ji the most in recent years, because its fruit quality is substantially better than The old kind such as bright treasured, Feng Xiang and deeply welcome by producers and consumers, but the nursery of this kind of new varieties is a difficult point.Mesh More front specialty strawberry seedling breeding companies are mainly heeled in the both sides nutrition hung by the stolon of pumping on overhead Alms bowl or cut stolon and carry out cutting propagation, although said method can breed substantial amounts of sub-Seedling, but the expense of taking a lot of work Time, and on stolon, early origin adventitious root is due to the longer gradually necrosis of exposed time, so causing the seedling of cutting propagation Rate is relatively low, seedling required time is longer, thus have impact on the popularization and application of the overhead seedling-raising technique of Fructus Fragariae Ananssae.Therefore, the most more For effectively changing into strawberry seedling the most rapidly, the most simply and efficiently by a large amount of stolons making overhead nursery produce It it is letter technical problem to be solved.
Summary of the invention
It is an object of the invention to provide and a kind of make the overhead stolon bred out change into grass quickly, simply, efficiently The new method of certain kind of berries seedling;
Present invention also offers that a kind of seedling is overhead bred at Fructus Fragariae Ananssae improved seeds " beauty and a chapter Ji " by said method Application in journey.
The purpose of the present invention can be achieved through the following technical solutions:
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon, prepares according to the following steps:
A, the induction of Fructus Fragariae Ananssae stolon: in 3~April Fructus Fragariae Ananssae maternal plant planted in the overhead substrate of 2m, in time Watering, apply fertilizer and prevent and treat possible pest and disease damage, the female Seedling of induction produces filial generation stolon, until stolon Seedling is sagging close to ground, On every stolon raw 8~9 stolon Seedlings;Strawberry cultivars is strawberry cultivars beauty and a chapter Ji for the main cultivation of current China;
B, the shearing of stolon and classification: shear off from maternal plant by Fructus Fragariae Ananssae stolon with shears, remove in stolon Between coupling part;Freshness according to the adventitious root on stolon stem is divided into two parts, is formed i.e. in early days and recently Stolon Seedling;Adventitious root on the stolon Seedling of early origin is removed, until seeing the healthy tissue of the inside;
C, the induction of aerial root: after being soaked by different types of for above two stolon Seedling, be respectively placed in different black plastic In Dai, it is advisable filling 2/3rds, is separately added into the water of 200~300mL, then seals bag mouth, be placed in more than 26~30 DEG C Space in, place about 6~14 days, period stirs stolon Seedling the most up and down, to ensure that the root of Seedling can touch water Point, the stolon Seedling that root the Bletilla striata (Thunb.ex A.Murray)Rchb.f. growing about more than 2cm is chosen;This step can be used for the root of inducing strawberry stolon Produce white root system for follow-up efficient sprouting and rooting;
D, transplanting and seedling: by having the stolon transplantation of seedlings of white adventitious root in substrate (peat or Vermiculitum), water After foot determines root water, carrying out covering 2~3 days with the sunshade net of black ground cloth or closeer, this step is steamed for the moisture reducing seedling Send out and promote the adventitious root acceleration of growth of Fructus Fragariae Ananssae stolon root and seedling, after time is up, opening covering, period according to The situation of temperature and weather is suitably watered, and after 1~2 week, Fructus Fragariae Ananssae stolon Seedling is substantially all and gives birth to more white of number Color root system, is changed into strawberry seedling by stolon the most efficiently.
The application during Fructus Fragariae Ananssae improved seeds seedling breeding of the new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon.
Beneficial effects of the present invention: the present invention uses overhead nursery and the mode of stolon rapid induction, it is possible to shorter In time, induction forms stolon in early days and recently and produces the fresh adventitious root of white, then promotes not to substrate by transplanting Determine root elongation and form new root system, promoting stolon to change into seedling efficiently.The present invention is simple, quick and makes efficiently Stolon tissue regeneration promoting root and is converted into seedling, and the seedling difference that Same Way is prepared at different batches, different time is little, and method is more Stable.
Accompanying drawing explanation
For the ease of it will be appreciated by those skilled in the art that the present invention is further illustrated below in conjunction with the accompanying drawings.
Fig. 1 is the white root system schematic diagram of induced synthesis on the stolon formed in the later stage;
Fig. 2 is stolon (adventitious root the is downright bad) schematic diagram of early origin;
Fig. 3 is to form stolon the later stage the white root system schematic diagram of tissue regeneration promoting;
Fig. 4 is to cultivate the new seedling schematic diagram formed.
Detailed description of the invention
Below in conjunction with example, the present invention is further illustrated, but is any limitation as the present invention never in any form, base In present invention teach that any change or improvement done, belong to protection scope of the present invention.
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon, the method comprises the steps:
A, the induction of Fructus Fragariae Ananssae stolon: according to the practical situation of this area, in yangtse-huaihe region general 3~April by Fructus Fragariae Ananssae Maternal plant is planted in the overhead substrate of about 2 meters, according to pollution-free food greenhouse strawberry high-level cultivating technical regulation (DB34/T 2014- 2013) regular watering, apply fertilizer and prevent and treat possible pest and disease damage.Induction maternal plant produces stolon, until stolon is sagging closely Face, on typically every stolon raw 8~9 stolon Seedlings time can start to shear stolon;
B, the shearing of stolon and classification: shear off from maternal plant by Fructus Fragariae Ananssae stolon with shears, remove stolon Seedling Middle coupling part.Freshness according to the adventitious root on stolon stem is divided into crawling of two parts, i.e. early origin Stem Seedling and two parts of stolon Seedling recently formed.Recently the stolon Seedling formed, the adventitious root root system on these stolons Color is white or milky, the freshest can directly apply this test;The stolon Seedling of early origin generally large and Raw adventitious root and have changed into brown color, major part root is due to the most exposed the most necrosis, these stolon Seedlings Forming root system by cutting propagation and need the longer time, the root position of part stolon is due to the parcel of dead adventitious root Being difficult to induction and produce new adventitious root, cuttage seedling probability is less, thus is likely to result in the waste of time and cost. Accordingly, the downright bad adventitious root of the stolon of early origin is removed, until seeing the healthy tissue of the inside, logical Crossing process induces the tissue of these health to form fresh white adventitious root.
C, the induction of aerial root: after being soaked by different types of for above two stolon Seedling, be respectively placed in black plastic bag In, be advisable filling 2/3rds, be separately added into the water of 200~300mL, then seal bag mouth, insulation be placed in 26~30 DEG C with On space in, place more than 6~14 days, period stirs stolon Seedling the most up and down, to ensure that the root of Seedling can touch Moisture;The stolon Seedling that root the Bletilla striata (Thunb.ex A.Murray)Rchb.f. growing about more than 2cm is chosen, until the root on essentially all of stolon The white root system that raw number differs.As Figure 1-4:
D, transplanting and seedling: will have the stolon transplantation of seedlings of fresh white adventitious root to substrate (Vermiculitum or peat) In, water after determining root water enough, carry out covering 2~3 days with the sunshade net of black ground cloth or closeer, after time is up, opens covering Thing, period suitably waters to keep substrate to moisten according to the situation of temperature and weather, Fructus Fragariae Ananssae stolon after 1~2 week It is substantially all and gives birth to the white root system that number is more, changed into strawberry seedling, planting percent by stolon the most efficiently Generally individually 85~more than 89%.
The new method of this efficient sprouting and rooting of Fructus Fragariae Ananssae stolon, may be used for Fructus Fragariae Ananssae improved Varieties by stolon relatively Rapidly and efficiently breeding of new varieties is realized in short time.Instant invention overcomes stolon in early days and produce adventitious root more difficulty not Foot, produces a small amount of aerial root by processing induction in early days stolon, then by transplanting to substrate and short-term dark culturing Mode makes the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon.The method has the features such as simple, convenient and stolon seedling speed is fast.This The time of inventive method induction stolon generation aerial root is relatively short and concentrates, and different time and different batches use this It is high and more stable that method produces filial generation stolon Seedling quality.
Above content is only citing made for the present invention and explanation, and affiliated those skilled in the art are to being retouched The specific embodiment stated makes various amendment or supplements or use similar mode to substitute, without departing from inventing or super More scope defined in the claims, all should belong to protection scope of the present invention.

Claims (6)

1. the new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon, it is characterised in that the method includes following seedling step:
A, the induction of Fructus Fragariae Ananssae stolon: planted by New Strawberry Variety maternal plant in overhead substrate, the female Seedling of induction produces filial generation and crawls Stem Seedling;
B, the shearing of stolon, classification and pretreatment: shear off from maternal plant by Fructus Fragariae Ananssae stolon with shears, remove stolon Middle coupling part;Stolon is divided into the stolon formed with the later stage in early days;By the worst on the stolon Seedling of early origin Dead adventitious root is removed, until seeing the healthy tissue of the inside;
C, the induction of aerial root: be respectively placed in black plastic bag after the stolon Seedling of above two different times is soaked, add Little water seals bag mouth, is placed in the space of 26 DEG C of temperature, places 6~14 natural law, and period constantly will grow the stolon of Bletilla striata (Thunb.ex A.Murray)Rchb.f. Choose, until the root of stolon Seedling is substantially all gives birth to the white root system that number differs;
D, transplanting and seedling: will have the stolon transplantation of seedlings of fresh white adventitious root in substrate, water after foot determines root water, use Covering is opened after covering 2~3 natural law, keeps substrate to moisten, and after fortnight, Fructus Fragariae Ananssae stolon is substantially all and gives birth to number The white root system that mesh is more, has been changed into strawberry seedling efficiently by stolon.
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon the most according to claim 1, it is characterised in that step A In, described New Strawberry Variety is beauty and a chapter Ji.
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon the most according to claim 1, it is characterised in that step B In, described classification is that the degree of necrosis according to the adventitious root on stolon is divided into two parts, the i.e. stolon of early origin Seedling and two parts of stolon Seedling recently formed.
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon the most according to claim 1, it is characterised in that step C In, described stolon Seedling is placed in black breakpoint plastic garbage bag, is advisable filling 2/3rds, adds 200~300mL Water, then seals bag mouth, and insulation is placed in the space of 26 DEG C~30 DEG C, places 6~14 days, and period stirs stolon the most up and down Seedling, chooses the stolon growing Bletilla striata (Thunb.ex A.Murray)Rchb.f., until essentially all stolon root all grow the white root system of about 2cm.
The new method of a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon the most according to claim 1, it is characterised in that step D In, described covering is that black the ground sunshade net of cloth or closeer, covering carry out covering 2~3 days, after opening covering, phase Between suitably water to keep the moistening degree of soil according to the situation of temperature and weather.
6. the new method of the arbitrary described a kind of efficient sprouting and rooting of Fructus Fragariae Ananssae stolon of claim 1-6 is at the excellent product of Fructus Fragariae Ananssae Plant the application in seedling reproductive process.
CN201610658292.7A 2016-08-11 2016-08-11 A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon Pending CN106258368A (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107343443A (en) * 2017-08-14 2017-11-14 高健强 A kind of Talinum crassifolium large-scale method for producing
CN107360846A (en) * 2017-09-01 2017-11-21 杭州贵妃家庭农场有限公司 A kind of method that stolon discarded using cultivated strawberry cultivates numerous seedling maternal plant
CN107593405A (en) * 2017-09-30 2018-01-19 江苏农林职业技术学院 The synthesis mating system of strawberry seedling
CN108770515A (en) * 2018-05-17 2018-11-09 中国农业大学 A kind of method and its application of collecting seedling of strawberry cuttage and seedling culture
CN109964755A (en) * 2019-05-15 2019-07-05 十堰市经济作物研究所 A method of improving Original Strawberry kind shoot survival percent and quality
CN110226468A (en) * 2019-07-23 2019-09-13 新疆农业科学院综合试验场 A kind of method for culturing seedlings and implantation methods of the open country strawberry being suitble to cold ground
CN110679306A (en) * 2019-10-21 2020-01-14 浙江省农业科学院 Method for concentrated cutting of strawberry substrate seedlings under simple facility condition
CN112166973A (en) * 2020-09-27 2021-01-05 郑州市蔬菜研究所 Rapid breeding method of strawberry seedlings
CN112930890A (en) * 2021-02-03 2021-06-11 农业农村部规划设计研究院 Strawberry stolon traction and cutting composite seedling culture method
CN114561422A (en) * 2022-02-28 2022-05-31 安徽农业大学 Induction method of transgenic hairy roots growing on strawberry stolons and application of transgenic hairy roots

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107343443A (en) * 2017-08-14 2017-11-14 高健强 A kind of Talinum crassifolium large-scale method for producing
CN107360846A (en) * 2017-09-01 2017-11-21 杭州贵妃家庭农场有限公司 A kind of method that stolon discarded using cultivated strawberry cultivates numerous seedling maternal plant
CN107593405A (en) * 2017-09-30 2018-01-19 江苏农林职业技术学院 The synthesis mating system of strawberry seedling
CN108770515A (en) * 2018-05-17 2018-11-09 中国农业大学 A kind of method and its application of collecting seedling of strawberry cuttage and seedling culture
CN109964755B (en) * 2019-05-15 2021-03-23 十堰市经济作物研究所 Method for improving survival rate and quality of strawberry stock seedlings
CN109964755A (en) * 2019-05-15 2019-07-05 十堰市经济作物研究所 A method of improving Original Strawberry kind shoot survival percent and quality
CN110226468A (en) * 2019-07-23 2019-09-13 新疆农业科学院综合试验场 A kind of method for culturing seedlings and implantation methods of the open country strawberry being suitble to cold ground
CN110226468B (en) * 2019-07-23 2021-10-15 新疆农业科学院综合试验场 Seedling raising method and planting method for open-field strawberries suitable for cold areas
CN110679306A (en) * 2019-10-21 2020-01-14 浙江省农业科学院 Method for concentrated cutting of strawberry substrate seedlings under simple facility condition
CN112166973A (en) * 2020-09-27 2021-01-05 郑州市蔬菜研究所 Rapid breeding method of strawberry seedlings
CN112930890A (en) * 2021-02-03 2021-06-11 农业农村部规划设计研究院 Strawberry stolon traction and cutting composite seedling culture method
CN114561422A (en) * 2022-02-28 2022-05-31 安徽农业大学 Induction method of transgenic hairy roots growing on strawberry stolons and application of transgenic hairy roots
CN114561422B (en) * 2022-02-28 2023-12-12 安徽农业大学 Induction method of transgenic hairy roots grown by strawberry stolon and application thereof

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Application publication date: 20170104