CN106255749A - 用于重组表达感兴趣多肽的新型脊椎动物细胞和方法 - Google Patents

用于重组表达感兴趣多肽的新型脊椎动物细胞和方法 Download PDF

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Publication number
CN106255749A
CN106255749A CN201580021660.0A CN201580021660A CN106255749A CN 106255749 A CN106255749 A CN 106255749A CN 201580021660 A CN201580021660 A CN 201580021660A CN 106255749 A CN106255749 A CN 106255749A
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interest
polypeptide
cell
interstitial
cells
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Chinese (zh)
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H·劳克斯
S·罗曼德
U·博登多夫
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21109—Matriptase (3.4.21.109)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • C—CHEMISTRY; METALLURGY
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Peptides Or Proteins (AREA)
CN201580021660.0A 2014-04-29 2015-04-29 用于重组表达感兴趣多肽的新型脊椎动物细胞和方法 Pending CN106255749A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201461985589P 2014-04-29 2014-04-29
US61/985,589 2014-04-29
US201461994310P 2014-05-16 2014-05-16
US61/994,310 2014-05-16
PCT/IB2015/053110 WO2015166427A2 (en) 2014-04-29 2015-04-29 Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest

Publications (1)

Publication Number Publication Date
CN106255749A true CN106255749A (zh) 2016-12-21

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US (2) US11512335B2 (cg-RX-API-DMAC7.html)
EP (3) EP3536776B1 (cg-RX-API-DMAC7.html)
JP (1) JP6636453B2 (cg-RX-API-DMAC7.html)
KR (1) KR102307278B1 (cg-RX-API-DMAC7.html)
CN (1) CN106255749A (cg-RX-API-DMAC7.html)
AU (1) AU2015254913B2 (cg-RX-API-DMAC7.html)
CA (1) CA2947198C (cg-RX-API-DMAC7.html)
DK (2) DK3137595T3 (cg-RX-API-DMAC7.html)
ES (2) ES2968879T3 (cg-RX-API-DMAC7.html)
IL (1) IL248184B (cg-RX-API-DMAC7.html)
LT (2) LT3536776T (cg-RX-API-DMAC7.html)
MX (1) MX368664B (cg-RX-API-DMAC7.html)
PL (2) PL3536776T3 (cg-RX-API-DMAC7.html)
PT (2) PT3536776T (cg-RX-API-DMAC7.html)
RU (1) RU2710726C2 (cg-RX-API-DMAC7.html)
SG (1) SG11201608358VA (cg-RX-API-DMAC7.html)
WO (1) WO2015166427A2 (cg-RX-API-DMAC7.html)

Cited By (1)

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CN111107870A (zh) * 2017-06-22 2020-05-05 催化剂生物科学公司 经修饰的膜型丝氨酸蛋白酶1（mtsp-1）多肽及其使用方法

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SG11201608358VA (en) 2014-04-29 2016-11-29 Novartis Ag Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest
EP3015475A1 (en) 2014-10-31 2016-05-04 Novartis AG Mammalian cells expressing cytomegalovirus antigens
WO2019180607A1 (en) * 2018-03-19 2019-09-26 Centre For Cellular And Molecular Platforms A vector, a host cell and a method thereof
US12559526B2 (en) * 2018-10-15 2026-02-24 The Regents Of The University Of California Complement component 1s (C1s) deficient cells for production of vaccines and biopharmaceutical proteins
US20220348632A1 (en) 2019-09-18 2022-11-03 Novartis Ag Nkg2d fusion proteins and uses thereof
AU2021215893A1 (en) * 2020-02-05 2022-08-25 Novartis Ag CHO cell expressing IL-15 heterodimers
CN115605592A (zh) * 2020-05-11 2023-01-13 基因泰克公司（Us） 用于治疗神经系统疾病的补体组分c1r抑制剂以及相关的组合物、系统和使用它们的方法
CN115551519A (zh) * 2020-05-11 2022-12-30 基因泰克公司 用于治疗神经系统疾病的补体组分c1s抑制剂以及相关的组合物、系统和使用它们的方法
US20250313878A1 (en) * 2022-05-19 2025-10-09 Novartis Ag IMPROVED PROTEIN PRODUCTION USING miRNA TECHNOLOGY

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Publication number Priority date Publication date Assignee Title
CN111107870A (zh) * 2017-06-22 2020-05-05 催化剂生物科学公司 经修饰的膜型丝氨酸蛋白酶1（mtsp-1）多肽及其使用方法

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KR20160147919A (ko) 2016-12-23
PT3137595T (pt) 2019-06-19
EP3137595B1 (en) 2019-03-13
CA2947198A1 (en) 2015-11-05
LT3137595T (lt) 2019-06-25
DK3536776T3 (da) 2024-01-15
IL248184B (en) 2021-03-25
EP4332231A2 (en) 2024-03-06
WO2015166427A2 (en) 2015-11-05
ES2731246T3 (es) 2019-11-14
JP2017514484A (ja) 2017-06-08
RU2016145928A3 (cg-RX-API-DMAC7.html) 2018-12-26
BR112016024534A2 (pt) 2017-10-10
EP4332231A3 (en) 2024-05-29
LT3536776T (lt) 2024-01-25
EP3536776B1 (en) 2023-10-25
AU2015254913B2 (en) 2018-05-17
AU2015254913A1 (en) 2016-10-27
PL3137595T3 (pl) 2019-09-30
US20230323419A1 (en) 2023-10-12
KR102307278B1 (ko) 2021-09-30
RU2016145928A (ru) 2018-05-29
MX2016014222A (es) 2017-05-04
RU2710726C2 (ru) 2020-01-10
MX368664B (es) 2019-10-10
PT3536776T (pt) 2024-01-12
ES2968879T3 (es) 2024-05-14
EP3137595A2 (en) 2017-03-08
JP6636453B2 (ja) 2020-01-29
PL3536776T3 (pl) 2024-03-25
IL248184A0 (en) 2016-11-30
SG11201608358VA (en) 2016-11-29
DK3536776T5 (da) 2024-09-02
EP3536776A1 (en) 2019-09-11
US11512335B2 (en) 2022-11-29
WO2015166427A3 (en) 2016-01-14
DK3137595T3 (da) 2019-06-17
CA2947198C (en) 2023-01-17
US20170044587A1 (en) 2017-02-16

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