CN106212543A - Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon - Google Patents
Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon Download PDFInfo
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- CN106212543A CN106212543A CN201610624309.7A CN201610624309A CN106212543A CN 106212543 A CN106212543 A CN 106212543A CN 201610624309 A CN201610624309 A CN 201610624309A CN 106212543 A CN106212543 A CN 106212543A
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- ganoderma
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- hypoxylon
- butt
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Agronomy & Crop Science (AREA)
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- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Dispersion Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the inhibitor suppressing butt Hypoxylon in Ganoderma substituting stuff cultivation, it is prepared by the following preparation method: 1) Folium Artemisiae Argyi and Rhizoma Acori Graminei are proportionally mixed, put into distillator, bottom distillator, heating is burnt or is passed through steam, when hot steam is filled in distillator, steam passes through condensing tube, it is introduced in condenser, then by oil water separator, collects quintessence oil and hydrosol；2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, obtain high speed dispersion emulsion, both inhibitor.Use the inhibitor suppressing butt Hypoxylon in the Ganoderma substituting stuff cultivation of the present invention, the growth of butt Hypoxylon can well be suppressed in the cultivation of Ganoderma, there is significant effect, the yield of Ganoderma can be strengthened simultaneously.
Description
Technical field
The present invention relates to field of edible fungus culture, particularly relate to Ganoderma substituting stuff cultivation suppresses the suppression of butt Hypoxylon
Agent.
Background technology
Ganoderma (formal name used at school: Ganoderma Lucidum Karst), also known as Ganoderma lucidum seu Japonicum, Herba mesonae chinensis, another name Ganoderma lucidum (Leyss. Ex Fr.) Karst., red sesame, wood spirit
Sesame, Ganoderma, Ganoderma lucisum, Ganoderma lucidum seu Japonicum, be Polyporaceae plant Ganoderma lucidum (Leyss. Ex Fr.) Karst. or the Herb of Ganoderma, for the son of On Polyporaceae Ganoderma
Entity.Profile is umbrella, cap kidney shape, semicircle or subcircular.Ganoderma is as the Chinese tradition having thousands of years medicinal histories
Valuable ingredient of Chinese medicine, has invigorating QI and tranquilization, effect of relieving cough and asthma, for dizziness sleeplessness, shortness of breath and palpitation, asthenia cough with asthma.Ganoderma possesses
The highest medical value, through scientific research institution's many decades modern pharmacology study confirm, Ganoderma for strengthen body immunity,
Regulation blood glucose, controls blood pressure, adjuvant therapy chemicotherapy, hepatoprotective, and the aspect such as hypnotic is respectively provided with significant curative effect.Ganoderma is many
Sugar is one of principle active component of Ganoderma medicinal function, is primarily present in glossy ganoderma cell wall inwall, and major part is beta glucan,
Its monosaccharide forms in addition to containing a large amount of glucoses, possibly together with a small amount of arabinose, xylose, fucose, and rhamnose, galactose and sweet
Dew sugar etc., they connect with glycosidic bonds such as (1-3), (1-4) and (1-6) mostly, have biological activity widely, as strengthened immunity
The effect such as power, removing human free radical, protection cardiovascular, radioprotective, the liver protecting, antitumor.Triterpenes is also having of Ganoderma
Effect one of composition, triterpenes contained by Ganoderma not under over one hundred kind, wherein based on tetracyclic triterpenes, the bitterness of Ganoderma and contained triterpene
Class is relevant, and human hepatoma cell is had cytotoxicity, also can suppress the release of histamine, has to protect the liver and makees with antiallergic etc.
With.
Owing to the Ganoderma of growth naturally is few, and containing substantial amounts of crude fibre and other impurity, and ganoderma polyoses content is the highest
(usually 2-5%), is not easy to directly as health food or pharmaceutical raw material, and therefore, many scientists carry out Ganoderma
Artificial culture and cultivation technique research.At present, the artificial generation (bottle) of Ganoderma expects that the technology such as cultivation, fermenting and producing obtain in succession
Merit, wherein, fermenting and producing is high to equipment requirements, and investment is big, is unfavorable for wide popularization and application；Ganoderma Lucidum is in China's origin
For a long time, from cultivation basswood to the development of substituting stuff cultivation technology, promote Ganoderma artificial culture on a large scale.
In cultivation of glossy ganoderma, often cause loss in various degree, wherein butt Hypoxylon because of the pollution of miscellaneous bacteria
(Hypoxylon anuulatum (Schw.) Mont.), be occurrence frequency and the extent of injury higher, it can be common that butt charcoal
Group, has another name called ring grain Hypoxylon, is commonly called as black treatment.Butt Hypoxylon, morphological characteristic: Stroma is little, hemispherical approximation warty, diameter is about
0.3-0.5cm, is connected with each other arrangement together, and the initial stage is coffee-like, after turn black, carbonaceous, ascus is subsphaeroidal, diameter 0.8-1.1mm,
Top evagination, the disk of the truncate formation in top diameter 350-500 μm, there are excipuliform microstome in its central authorities.Ascus is cylindric, has spore
Part 60-75 μ m 5-6 μm, shank long 35-55 μm.Spore is without tabula, brown, and inequilateral is oval, 7-9 μ m 3-4.5 μm.
Classification position: carbon angle bacteria mesh, Xylariaceae.Ecological habit: be born on the wooden of broadleaf tree and bark.All living creatures.Cause broad-leaved
The trees such as tree are wooden rotten, usually fungus cultivation, Lentinus Edodes Duan Mu on raised growth affect its yield, be considered as " miscellaneous bacteria ".Many
Betiding south, its reproductive capacity is strong.Occur mainly in the longitudinal furrow of mushroom (ear), Ganoderma outer surface of wood skin, formed like Semen phaseoli radiati or Semen Glycines
The black particle of size, rough surface, matter is hard, and time serious, black particle is often linked to be sheet.This bacterium readily occurs in moist concealment
On the Duan Mu at place, reproductive capacity is strong.After Duan Mu is endangered by it, it is suppressed that the growth of hypha of edible fungus and spreading, cambium layer becomes ash
Black, defines " unshakable in one's determination ", inhales and does not intake point.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of with low cost, effect obvious Ganoderma generation material
Cultivation suppresses the inhibitor of butt Hypoxylon.
The present invention is achieved by the following technical solution:
Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon, is prepared by the following preparation method:
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei proportionally being mixed, put into distillator, bottom distillator, heating is burnt or is passed through steaming
Vapour, when hot steam is filled in distillator, steam passes through condensing tube, is introduced in condenser, then passes through oil-water separation
Device, collects quintessence oil and hydrosol；
2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, obtain
High speed dispersion emulsion, had both obtained inhibitor.
Step 1 of the present invention) described in Folium Artemisiae Argyi and Rhizoma Acori Graminei proportionally mix, preferably according to 1-3:1-2 part by weight mix
Close；
Step 2) described in high speed dispersion emulsifying, preferably rotating speed 2000-3000r/min, time 10-20min.
Ganoderma substituting stuff cultivation of the present invention suppresses the inhibitor of butt Hypoxylon, in use, preferably dilutes
Using after 100-200 times, after mushroom house cleans out sterilization, before putting into bacterium rod, the present invention after mushroom house is sprayed dilution presses down
Preparation, after putting into bacterium rod, at vegetative stage, sprays inhibitor of the present invention 1-2 time.
Compared with prior art, present invention have the advantage that
1, in prior art, Ganoderma mycelium growth stage prevents butt Hypoxylon from polluting, general prevention and controls: one is
Spray wine section wood surface and section with 0.1% potassium permanganate or 500 times of 70% thiophanate methyl before short section of wood pack sterilizing, can press down
The sprouting of butt processed group bacterium spore and mycelial growth；Two is to find miscellaneous bacteria the initial stage, available dense Calx copper sulfate mixed solution (Calx
8g, copper sulfate 8g, water 100ml) spray or smear, or after being struck off by Stroma and burning, the above-mentioned medicinal liquid of shaving brushing prevents regeneration；Three
If being the killed serious formation of bacterium material " unshakable in one's determination ", should burn.Cure the symptoms, not the disease with upper type, and the present invention uses and plants at Ganoderma generation material
Training is sprayed inhibitor and is reached to suppress the purpose of butt Hypoxylon in cultivating, use Chinese herbal medicine sterilization, it is to avoid chemical bactericide
Use, Environmental Safety, effective.
2, quintessence oil is from the flower of plant, leaf, stem, root or fruit, by steam distillation, squeezing and pressing method, cold-maceration or
Solvent extraction method refine extraction volatile flavor, quintessence oil essence can anti-communicable disease, to antibacterium, virus, mycete, can prevent
Inflammation.Hydrosol, also known as crystal's oil (Hydrolat), refers to that quintessence oil, during distillation extraction, is separated when refining quintessence oil
A kind of 100% saturated distillation stock solution, be a kind of side-product of quintessence oil, composition is natural pure, and fragrance is light pleasant.Hydrosol is just
It is the condensed water solution of virtue treatment plant distillation gained.During distillation extraction, profit can separate, and because density is different, quintessence oil can float
Floating over above, moisture is then deposited in following, and these moisture are just hydrosol.In hydrosol in addition to containing a small amount of derived essential oil, also
Containing the water-soluble substances in whole plants.Have the hydrosol of absolutely plant water-soluble material, mineral nutrients contained by it
(such as tannic acid and flavonoid) is that quintessence oil lacks.But quintessence oil has a biggest problem, waving of quintessence oil in actual use
The property sent out is very strong, and once ingress of air will quickly volatilize, and based on this reason, quintessence oil must be with the dark-colored bottle storage that can seal
Deposit, once open use, also to cover back lid as early as possible.And the present invention is by carrying the quintessence oil in Chinese herbal medicine, hydrosol from plant
After taking, the quintessence oil, the hydrosol that obtain mix after passing through high speed dispersion emulsifying, and the hydrosol after emulsifying can wrap up quintessence oil, prevent
The volatilization of quintessence oil.The present invention, by using Folium Artemisiae Argyi Rhizoma Acori Graminei mixture at Ganoderma substituting stuff cultivation in cultivating, can give full play to Chinese mugwort
The bacteria resistance function of leaf Rhizoma Acori Graminei, reduces culture medium and is truncated the harm that Hypoxylon pollutes.
Detailed description of the invention
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1:
Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon, is prepared by the following preparation method:
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei are mixed according to 1:2 part by weight, put into distillator, bottom distillator, heating burning
Or it is passed through steam, when hot steam is filled in distillator, steam passes through condensing tube, is introduced in condenser, then passes through
Oil water separator, collects quintessence oil and hydrosol；
2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, rotating speed
2000r/min, time 20min, obtain high speed dispersion emulsion, both obtained inhibitor.
Embodiment 2:
Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon, is prepared by the following preparation method:
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei are mixed according to 3:1 part by weight, put into distillator, bottom distillator, heating burning
Or it is passed through steam, when hot steam is filled in distillator, steam passes through condensing tube, is introduced in condenser, then passes through
Oil water separator, collects quintessence oil and hydrosol；
2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, rotating speed
3000r/min, time 10min, obtain high speed dispersion emulsion, both obtained inhibitor.
Embodiment 3:
Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon, is prepared by the following preparation method:
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei are mixed according to 2:1 part by weight, put into distillator, bottom distillator, heating burning
Or it is passed through steam, when hot steam is filled in distillator, steam passes through condensing tube, is introduced in condenser, then passes through
Oil water separator, collects quintessence oil and hydrosol；
2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, rotating speed
2500r/min, time 15min, obtain high speed dispersion emulsion, both obtained inhibitor.
Contrast experiment
By to embodiment and comparative example, carry out cultivation of glossy ganoderma experiment at identical conditions, contrast experiment uses
Bacterium its Ganoderma substituting stuff cultivation culture medium prescription of rod, preparation process are identical, and the culture environment of bacterium rod is identical.
The agent that the embodiment of the present invention 3 is inhibited uses after diluting 100,150,200 times respectively, cleans out in mushroom house and disappears
After poison, before putting into bacterium rod, mushroom house is sprayed the inhibitor of the present invention after dilution, after putting into bacterium rod, in vegetative stage, spray
Spill inhibitor of the present invention 2 times；
Comparative example 1: only use Folium Artemisiae Argyi, preparation method is with embodiment 3.
Comparative example 2: raw material, with embodiment 3, adds 10 times of water, decocts the extracting solution obtained after extracting.
Comparative example 3: do not use inhibitor of the present invention.
Statistics be truncated Hypoxylon pollute situation:
Table 1: pollution condition unit: %
By contrast, Ganoderma substituting stuff cultivation of the present invention suppresses the inhibitor of butt Hypoxylon, can well be Ganoderma
Cultivation in suppress butt Hypoxylon growth, there is significant effect, and the yield of Ganoderma compare use " many bacterium
Spirit " chemosterilant, increase of production more than 20%.
Claims (4)
1. Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon, it is characterised in that: it is prepared by the following preparation method:
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei proportionally being mixed, put into distillator, bottom distillator, heating is burnt or is passed through steam,
When hot steam is filled in distillator, steam passes through condensing tube, is introduced in condenser, then by oil water separator,
Collect quintessence oil and hydrosol；
2) high speed dispersion emulsifying: step 1) quintessence oil that obtains and hydrosol, put into high speed dispersion mulser and process, obtain at a high speed
Dispersion and emulsion thing, had both obtained inhibitor.
Ganoderma substituting stuff cultivation the most according to claim 1 suppresses the inhibitor of butt Hypoxylon, it is characterised in that: step
1) Folium Artemisiae Argyi and Rhizoma Acori Graminei described in proportionally mix, and mix according to 1-3:1-2 part by weight.
Ganoderma substituting stuff cultivation the most according to claim 1 suppresses the inhibitor of butt Hypoxylon, it is characterised in that: step
2) the high speed dispersion emulsifying described in, rotating speed 2000-3000r/min, time 10-20min.
Ganoderma substituting stuff cultivation the most according to claim 1 suppresses the inhibitor of butt Hypoxylon, it is characterised in that: obtain
Inhibitor, dilute and use after 100-200 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610624309.7A CN106212543A (en) | 2016-07-29 | 2016-07-29 | Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon |
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| CN201610624309.7A CN106212543A (en) | 2016-07-29 | 2016-07-29 | Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon |
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| CN201610624309.7A Pending CN106212543A (en) | 2016-07-29 | 2016-07-29 | Ganoderma substituting stuff cultivation suppresses the inhibitor of butt Hypoxylon |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104336101A (en) * | 2014-09-26 | 2015-02-11 | 桂林丰茂源农业技术开发有限公司 | Air sterilization method for edible mushroom culture room |
| CN104886178A (en) * | 2015-04-28 | 2015-09-09 | 陈文滨 | Natural plant sterilization smoking agent and preparation method thereof |
-
2016
- 2016-07-29 CN CN201610624309.7A patent/CN106212543A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104336101A (en) * | 2014-09-26 | 2015-02-11 | 桂林丰茂源农业技术开发有限公司 | Air sterilization method for edible mushroom culture room |
| CN104886178A (en) * | 2015-04-28 | 2015-09-09 | 陈文滨 | Natural plant sterilization smoking agent and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| 刘强，刘莉: "《中药新产品开发》", 31 March 2013 * |
| 张鹏，谢培镇，杨卓鸿，等: "《艾叶油作为天然抗菌剂的研究进展》", 《广东化工》 * |
| 薛广波: "《现代消毒学》", 31 July 2002 * |
| 金丽泰: "《天然药物提取分离工艺学》", 31 October 2011 * |
| 陈丽新，黄卓忠，覃晓娟: "《食用菌生产三种室内空气灭菌方法比较试验》", 《食用菌》 * |
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