CN106164292B - 使用SOMAmer通过基于荧光的检测方法检测样品中微生物的方法 - Google Patents

使用SOMAmer通过基于荧光的检测方法检测样品中微生物的方法 Download PDF

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Publication number
CN106164292B
CN106164292B CN201580009181.7A CN201580009181A CN106164292B CN 106164292 B CN106164292 B CN 106164292B CN 201580009181 A CN201580009181 A CN 201580009181A CN 106164292 B CN106164292 B CN 106164292B
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Prior art keywords
sample
somamer
fluorescence
seq
staphylococcus aureus
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Chinese (zh)
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CN106164292A (zh
Inventor
A.鲍姆斯蒂姆勒
F.马克-克勒谢尔
D.莱曼
U.奥克斯纳
N.詹吉克
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Private Placement Of Proteosome Operating Co
Merck Patent GmbH
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Private Placement Of Proteosome Operating Co
Merck Patent GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580009181.7A 2014-02-21 2015-02-20 使用SOMAmer通过基于荧光的检测方法检测样品中微生物的方法 Active CN106164292B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14290047.1 2014-02-21
EP14290046 2014-02-21
EP14290047 2014-02-21
EP14290046.3 2014-02-21
PCT/EP2015/000393 WO2015124315A1 (en) 2014-02-21 2015-02-20 A method of detecting a microorganism in a sample by a fluorescence based detection method using somamers

Publications (2)

Publication Number Publication Date
CN106164292A CN106164292A (zh) 2016-11-23
CN106164292B true CN106164292B (zh) 2025-04-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580009181.7A Active CN106164292B (zh) 2014-02-21 2015-02-20 使用SOMAmer通过基于荧光的检测方法检测样品中微生物的方法

Country Status (6)

Country Link
US (1) US10526663B2 (https=)
EP (1) EP3108007B1 (https=)
JP (2) JP2017506912A (https=)
CN (1) CN106164292B (https=)
ES (1) ES2736474T3 (https=)
WO (1) WO2015124315A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015126769A1 (en) * 2014-02-18 2015-08-27 Somalogic, Inc. Compositions and methods for detecting microorganisms
JP7314456B2 (ja) * 2018-12-06 2023-07-26 株式会社マツモト精密工業 牛乳の検査装置
JP7286070B2 (ja) * 2021-01-31 2023-06-05 株式会社バランス・イースト 検査システム、検査方法、プログラム、およびコンピュータが読み取り可能な記憶媒体
WO2023287511A2 (en) * 2021-06-02 2023-01-19 Board Of Regents, The University Of Texas System Methods and compositions related to engineered biosensors

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US9562900B2 (en) 2008-02-21 2017-02-07 Otc Biotechnologies, Llc Methods and compositions of nucleic acid ligands for detection of foodborne and waterborne pathogens
US20150056627A1 (en) 2011-10-31 2015-02-26 Dupont Nutrition Biosciences Aps Aptamers
WO2015126769A1 (en) * 2014-02-18 2015-08-27 Somalogic, Inc. Compositions and methods for detecting microorganisms

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery;Larry Gold et al.;《 PLOS ONE》;20101231;摘要，第9-15页 *
Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers;Urs A. Ochsner et al.;《Diagnostic Microbiology and Infectious Disease》;20131231;摘要，2.2-2.7部分，表1、3，图1，第284页右栏 *
From SOMAmer-Based Biomarker Discovery to Diagnostic and Clinical Applications: A SOMAmer-Based, Streamlined Multiplex Proteomic Assay;Stephan Kraemer et al.;《PLOS ONE》;20111031;第1-3页，图1 *
Stephan Kraemer et al..From SOMAmer-Based Biomarker Discovery to Diagnostic and Clinical Applications: A SOMAmer-Based, Streamlined Multiplex Proteomic Assay.《PLOS ONE》.2011,第1-3页，图1. *
Urs A. Ochsner et al..Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers.《Diagnostic Microbiology and Infectious Disease》.2013,摘要，2.2-2.7部分，表1、3，图1，第284页右栏. *

Also Published As

Publication number Publication date
ES2736474T3 (es) 2020-01-02
WO2015124315A1 (en) 2015-08-27
US10526663B2 (en) 2020-01-07
JP2017506912A (ja) 2017-03-16
EP3108007B1 (en) 2019-04-17
CN106164292A (zh) 2016-11-23
JP6989585B2 (ja) 2022-01-05
US20170051337A1 (en) 2017-02-23
EP3108007A1 (en) 2016-12-28
JP2020072679A (ja) 2020-05-14

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