ES2736474T3 - Un método para detectar un microorganismo en una muestra mediante un método de detección basado en fluorescencia usando SOMAmers - Google Patents

Un método para detectar un microorganismo en una muestra mediante un método de detección basado en fluorescencia usando SOMAmers Download PDF

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Publication number
ES2736474T3
ES2736474T3 ES15706159T ES15706159T ES2736474T3 ES 2736474 T3 ES2736474 T3 ES 2736474T3 ES 15706159 T ES15706159 T ES 15706159T ES 15706159 T ES15706159 T ES 15706159T ES 2736474 T3 ES2736474 T3 ES 2736474T3
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Spain
Prior art keywords
seq
sample
somamer
nucleotide sequence
somamers
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Active
Application number
ES15706159T
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English (en)
Spanish (es)
Inventor
Anne Baumstummler
Frédéric Marc-Kreucher
David Lehmann
Urs Ochsner
Nebojsa Janjic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Somalogic Inc
Original Assignee
Merck Patent GmbH
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES15706159T 2014-02-21 2015-02-20 Un método para detectar un microorganismo en una muestra mediante un método de detección basado en fluorescencia usando SOMAmers Active ES2736474T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14290046 2014-02-21
EP14290047 2014-02-21
PCT/EP2015/000393 WO2015124315A1 (en) 2014-02-21 2015-02-20 A method of detecting a microorganism in a sample by a fluorescence based detection method using somamers

Publications (1)

Publication Number Publication Date
ES2736474T3 true ES2736474T3 (es) 2020-01-02

Family

ID=52577818

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15706159T Active ES2736474T3 (es) 2014-02-21 2015-02-20 Un método para detectar un microorganismo en una muestra mediante un método de detección basado en fluorescencia usando SOMAmers

Country Status (6)

Country Link
US (1) US10526663B2 (https=)
EP (1) EP3108007B1 (https=)
JP (2) JP2017506912A (https=)
CN (1) CN106164292B (https=)
ES (1) ES2736474T3 (https=)
WO (1) WO2015124315A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015126769A1 (en) * 2014-02-18 2015-08-27 Somalogic, Inc. Compositions and methods for detecting microorganisms
JP7314456B2 (ja) * 2018-12-06 2023-07-26 株式会社マツモト精密工業 牛乳の検査装置
JP7286070B2 (ja) * 2021-01-31 2023-06-05 株式会社バランス・イースト 検査システム、検査方法、プログラム、およびコンピュータが読み取り可能な記憶媒体
WO2023287511A2 (en) * 2021-06-02 2023-01-19 Board Of Regents, The University Of Texas System Methods and compositions related to engineered biosensors

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US9562900B2 (en) 2008-02-21 2017-02-07 Otc Biotechnologies, Llc Methods and compositions of nucleic acid ligands for detection of foodborne and waterborne pathogens
US20150056627A1 (en) 2011-10-31 2015-02-26 Dupont Nutrition Biosciences Aps Aptamers
WO2015126769A1 (en) * 2014-02-18 2015-08-27 Somalogic, Inc. Compositions and methods for detecting microorganisms

Also Published As

Publication number Publication date
WO2015124315A1 (en) 2015-08-27
US10526663B2 (en) 2020-01-07
JP2017506912A (ja) 2017-03-16
EP3108007B1 (en) 2019-04-17
CN106164292A (zh) 2016-11-23
JP6989585B2 (ja) 2022-01-05
CN106164292B (zh) 2025-04-22
US20170051337A1 (en) 2017-02-23
EP3108007A1 (en) 2016-12-28
JP2020072679A (ja) 2020-05-14

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