CN106148260A - 高产乙酰氨基葡萄糖的重组枯草芽孢杆菌及其构建方法 - Google Patents
高产乙酰氨基葡萄糖的重组枯草芽孢杆菌及其构建方法 Download PDFInfo
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- CN106148260A CN106148260A CN201610513758.4A CN201610513758A CN106148260A CN 106148260 A CN106148260 A CN 106148260A CN 201610513758 A CN201610513758 A CN 201610513758A CN 106148260 A CN106148260 A CN 106148260A
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- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01002—Glucokinase (2.7.1.2)
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- C12Y503/00—Intramolecular oxidoreductases (5.3)
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CN201610513758.4A CN106148260B (zh) | 2016-07-01 | 2016-07-01 | 高产乙酰氨基葡萄糖的重组枯草芽孢杆菌及其构建方法 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287144A (zh) * | 2017-07-19 | 2017-10-24 | 江南大学 | 一种代谢改造的枯草芽孢杆菌生物转化细胞及其制备方法与应用 |
CN107699535A (zh) * | 2017-11-08 | 2018-02-16 | 光明乳业股份有限公司 | 一种诱导合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN107805640A (zh) * | 2017-10-13 | 2018-03-16 | 中国农业科学院植物保护研究所 | 一种提高链霉菌次级代谢产物产量的方法 |
CN107805622A (zh) * | 2017-11-08 | 2018-03-16 | 光明乳业股份有限公司 | 一种合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN108424869A (zh) * | 2018-04-04 | 2018-08-21 | 江南大学 | 一种促进枯草芽孢杆菌乙酰氨基葡萄糖合成的方法 |
CN109735479A (zh) * | 2019-01-30 | 2019-05-10 | 光明乳业股份有限公司 | 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104498517A (zh) * | 2014-11-29 | 2015-04-08 | 滨州市金朗生物科技有限公司 | 一种高产n-乙酰氨基葡萄糖大肠杆菌的构建及应用方法 |
CN104928333A (zh) * | 2015-07-07 | 2015-09-23 | 江南大学 | 一种敲除glcK促进枯草芽孢杆菌合成乙酰氨基葡萄糖的方法 |
CN105255803A (zh) * | 2015-11-10 | 2016-01-20 | 江南大学 | 一种高效合成乙酰氨基葡萄糖的重组枯草芽孢杆菌 |
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2016
- 2016-07-01 CN CN201610513758.4A patent/CN106148260B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104498517A (zh) * | 2014-11-29 | 2015-04-08 | 滨州市金朗生物科技有限公司 | 一种高产n-乙酰氨基葡萄糖大肠杆菌的构建及应用方法 |
CN104928333A (zh) * | 2015-07-07 | 2015-09-23 | 江南大学 | 一种敲除glcK促进枯草芽孢杆菌合成乙酰氨基葡萄糖的方法 |
CN105255803A (zh) * | 2015-11-10 | 2016-01-20 | 江南大学 | 一种高效合成乙酰氨基葡萄糖的重组枯草芽孢杆菌 |
Non-Patent Citations (3)
Title |
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MEIXI LING等: "Combinatorial promoter engineering of glucokinase and phosphoglucoisomerase for improved N-acetylglucosamine production in Bacillus subtilis", 《BIORESOURCE TECHNOLOGY》 * |
YANFENG LIU等: "Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production", 《METABOLIC ENGINEERING》 * |
YANQIU ZHU等: "An optimal glucose feeding strategy integrated with step-wise regulation of the dissolved oxygen level improves N-acetylglucosamine production in recombinant Bacillus subtilis", 《BIORESOURCE TECHNOLOGY》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287144A (zh) * | 2017-07-19 | 2017-10-24 | 江南大学 | 一种代谢改造的枯草芽孢杆菌生物转化细胞及其制备方法与应用 |
CN107287144B (zh) * | 2017-07-19 | 2020-01-21 | 江南大学 | 一种代谢改造的枯草芽孢杆菌生物转化细胞及其制备方法与应用 |
CN107805640A (zh) * | 2017-10-13 | 2018-03-16 | 中国农业科学院植物保护研究所 | 一种提高链霉菌次级代谢产物产量的方法 |
CN107805640B (zh) * | 2017-10-13 | 2020-11-03 | 中国农业科学院植物保护研究所 | 一种提高链霉菌次级代谢产物产量的方法 |
CN107699535A (zh) * | 2017-11-08 | 2018-02-16 | 光明乳业股份有限公司 | 一种诱导合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN107805622A (zh) * | 2017-11-08 | 2018-03-16 | 光明乳业股份有限公司 | 一种合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN107805622B (zh) * | 2017-11-08 | 2021-04-30 | 光明乳业股份有限公司 | 一种合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN108424869A (zh) * | 2018-04-04 | 2018-08-21 | 江南大学 | 一种促进枯草芽孢杆菌乙酰氨基葡萄糖合成的方法 |
CN108424869B (zh) * | 2018-04-04 | 2020-05-08 | 江南大学 | 一种促进枯草芽孢杆菌乙酰氨基葡萄糖合成的方法 |
CN109735479A (zh) * | 2019-01-30 | 2019-05-10 | 光明乳业股份有限公司 | 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用 |
CN109735479B (zh) * | 2019-01-30 | 2022-04-01 | 光明乳业股份有限公司 | 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用 |
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