CN106137786A - A kind of anti-ageing compositions of hyaluronic acid and its preparation method and application - Google Patents
A kind of anti-ageing compositions of hyaluronic acid and its preparation method and application Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of hyaluronic acid compositions with senile-resistant efficacy and preparation thereof and application.Described compositions contains and a kind of hydrolyzes hyaluronic acid or its salt and the hyaluronic acid of two kinds of weight average molecular weight or its salt.Through multinomial tests such as skin moisture content, skin elasticity and wrinkle depths, finally determine the optimum proportioning with notable anti-ageing effect.Wherein hydrolysis hyaluronic acid or its salt are 60% ~ 62% in weight ratio shared by compositions；Low-molecular-weight hyaluronic acid or its salt weight ratio in the composition are 18% ~ 20%；High molecular weight hyaluronic acid or its salt weight ratio in the composition are 18% ~ 20%.Said composition has three-dimensional moisturizing, removes the anti-aging effects such as free radical, cytothesis, recovery skin elasticity, can be used in cosmetics and the cleaning product of form of ownership.
Description
Technical field
The present invention relates to hyaluronic acid and derivant technical field thereof, particularly to a kind of anti-ageing compositions of hyaluronic acid,
Further relate to the preparation method and applications of the anti-ageing compositions of described hyaluronic acid.
Background technology
Skin aging shows as skin volume and elastic loss.Skin volume loss is to cause due to skin HA disappearance
The dermal filler dehydrations such as collagen are hardened and are caused；And owing to the loss of skin volume causes skin to become coarse, lose bullet
Property, wrinkle occurs.Therefore, hyaluronic acid is most important for the healthy youth of skin.
Hyaluronic acid (hyaluronic acid, HA) is a kind of acidic mucopolysaccharide, by NAG and D-glucose
The glycosaminoglycans without branched high molecular that aldehydic acid disaccharidase recurring unit is constituted, is present in animal tissue cell's interstitial and some antibacterial
In pod membrane.Hyaluronic acid is widely used in the fields such as medicine, cosmetics, food, and molecular weight is generally 105-107Da (dalton).
It is known that HA is nature moisturizing factor, the HA that molecular weight is high has the premium properties of water conservation moisturizing, film forming, lubrication.Low molecule
Amount HA is less because of its relative molecular mass, and the outer used time preferably can be absorbed by skin, promotes supply and the refuse row of skin-nourishing
Let out, thus prevent skin aging, reach the effect of deep moisturizing and looks improving and the skin nourishing, and may additionally facilitate epidermis cell propagation and
Differentiation, scavenging activated oxygen, repair daylight or the skin of uv damage.
Prior art also have many for preventing skin aging, the hyaluronic acid compositions of scavenging activated oxygen, as follows:
CN 105233270A discloses a kind of pharmaceutical composition for the treatment of water light, by high, medium and low, 4 kinds of molecular weight of oligomerization
Hyaluronic acid and collagen protein, epidermal growth factor and the gel of adjuvant composition.4 kinds of molecular weight are respectively 1300～2000kDa,
500～1000kDa, 50～300kDa, 3～8kDa, they optimum content in gel are respectively 0.1%～0.2%,
0.1%～0.2%, 0.1%～0.5%, 0.2%～0.6%.Documents compositions is possibly together with collagen protein, epidermal growth
The well-known activity of fighting against senium composition of the factor two kinds.Injected by face and improve dry, microgroove, the skin problem such as aging.
Hyaluronic acid, collagen protein and epidermal growth factor are delivered directly in skin (water optoinjection by injection by documents
The degree of depth is generally at skin corium), for xerosis cutis, microgroove, the improvement of the skin problem such as aging, derive from injection to a certain extent
The effect filled, by expanding skin volume, and directly replenishing collagen and somatomedin, so that skin collagen
Content dramatically increases, and by the somatomedin proliferation function to cell, reaches the effect to resisting age of skin.
Make full use of the performance of hyaluronic acid itself, develop a kind of simple hyaluronic acid with fine anti-ageing effect
Compositions, makes full use of hyaluronic acid performance and the development of industry, all has more profound significance.
Summary of the invention
In order to solve hyaluronic acid performance in anti-ageing not sufficiently problem, the application in above prior art
The significant hyaluronic acid of anti-aging effects such as open a kind of three-dimensional moisturizing, removing free radical, cytothesis, recovery skin elasticity resists
Decline compositions.
It is a further object to provide the preparation method of the anti-ageing compositions of described hyaluronic acid.
It is also another object of the present invention to provide the application of the anti-ageing compositions of described hyaluronic acid.
The present invention is obtained through the following steps:
A kind of anti-ageing compositions of hyaluronic acid, raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 60%～62% of 3～8kDa, molecular weight be 300～500kDa saturating
The acid of bright matter or its salt 18%～20%, molecular weight is hyaluronic acid or its salt 18%～20% of 1000～1200kDa.
The anti-ageing compositions of described hyaluronic acid, raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 60%～62% of 7kDa, molecular weight be 320kDa hyaluronic acid or
Its salt 18%～20%, molecular weight is hyaluronic acid or its salt 18%～20% of 1100kDa.
The anti-ageing compositions of described hyaluronic acid, raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 62% of 7kDa, and molecular weight is hyaluronic acid or its salt of 320kDa
18%, molecular weight is hyaluronic acid or its salt 20% of 1100kDa.
The anti-ageing compositions of described hyaluronic acid, described hydrolysis hyaluronic acid or its salt are with bacillus cereus
Hyaluronic acid or its salt are degraded and to be obtained by the bacillus cereus hyaluronidase that A50CGMCCNO.5744 fermentation culture obtains
Enzyme action oligomerization hyaluronic acid or its salt.
Oligomerization hyaluronic acid refers to that molecular weight is less than the hyaluronic acid of 10kDa.Research shows, molecular weight is to hyaluronic acid
Activity has a significant impact.Oligomerization hyaluronic acid can penetrate into corium, improves the lasting moistening effect of skin, has mild dilation simultaneously
Blood capillary, increases blood circulation, improves intermediate supersession, promotion skin-nourishing Absorption, also have stronger wrinkle-chasing function,
Skin elasticity, delay skin aging can be increased.Additionally, hydroxyl, carboxyl and other polar groups in oligomerization hyaluronic acid molecules of salt
Group can form hydrogen bond with hydrone and combine substantial amounts of moisture, and Water-saving effect is obvious, and therefore oligomerization hyaluronate can be used for preventing
In solarization, defying age, moisture-keeping cosmetics.
Enzyme action oligomerization hyaluronic acid i.e. hydrolyzes hyaluronic acid, for Hua Xi Fu Ruida biomedical company's independent research and produce
HA product, use the hyaluronidase that obtains of fermentable that high molecular weight hyaluronic acid or its salt are degraded, enzyme is lived
Height, mild condition, simple to operate, non-environmental-pollution；And the enzyme action oligomerization hyaluronic acid obtained or its salt structural integrity.With it
The oligomerization hyaluronic acid that his producer chemical method produces is compared, and enzyme action oligomerization hyaluronic acid has that percutaneous absorbability is good, purity is high, nothing
The advantages such as cytotoxicity, oxidation resistance are strong.It addition, test confirms, enzyme action oligomerization hyaluronic acid also has removing free radical, repaiies
Multiple shine after the effect such as cell, sun-proof and Angiogensis, can be used on cosmetics, food and field of medicaments, have a extensive future.
But, for skin aging, keep and improve moisture of skin, skin nutrition, removing skin free radical and improve skin
Skin elasticity is all particularly important, and only enzyme action oligomerization hyaluronic acid can not be fully achieved this purpose.So, this area is urgent
Need a kind of compositions can reach to keep and improve moisture of skin, skin nutrition, removing skin free radical, repair damaging cells
And improve the defying age effectiveness of skin elasticity.
Described hyaluronate or hydrolysis hyaluronate be hyaluronic acid or hydrolysis the sodium salt of hyaluronic acid, potassium salt,
Magnesium salt, calcium salt or zinc salt.
The preparation method of the anti-ageing compositions of described hyaluronic acid: by the hyaluronic acid of two kinds of molecular weight or its salt and hydrolysis
Hyaluronic acid or its salt mix according to weight ratio, stir, to obtain final product.
The application in preparing anti-aging product of the described hyaluronic acid anti-ageing compositions.
Described defying age is moisturizing, removes free radical, cytothesis, recovery skin elasticity.
Described anti-aging product be toner/smoothing toner, essence, curry, emulsion, cream, facial film, color make-up, fancy soap, cleansing milk or
Bath gel.
Beneficial effects of the present invention:
1) the anti-ageing compositions of hyaluronic acid contains hydrolysis hyaluronic acid or its salt and hyaluronic acid or its salt, by different molecular
Amount hyaluronic acid or its salt combine with hydrolysis hyaluronic acid or its salt (i.e. enzyme action oligomerization hyaluronic acid or its salt), have both had high score
Sub-HA skin protection, the essential effectiveness of natural moisture preserving, have again Middle molecule HA easily absorb, the characteristic of skin nutrition, separately there is enzyme
Cut oligomerization hyaluronic acid Transdermal absorption, remove free radical, cytothesis, anti-ageing biological activity of waiting for a long time, can Transdermal absorption, and have
Three-dimensional moisturizing, removing free radical, reparation damaging cells and the defying age effectiveness of recovery skin elasticity；
2) make full use of the different skin care effect of different molecular weight section HA, through skin moisture content, skin elasticity and
The multinomial test such as wrinkle depth, finally determines the optimum proportioning with notable anti-ageing effect, fully excavates the anti-of hyaluronic acid
Decline effect, provides more probability for hyaluronic acid goods development.
Accompanying drawing explanation
Fig. 1 moisture of skin increments measurement result,
Fig. 2 skin water loss reduction amount measurement result,
Fig. 3 wrinkle mean depth test result,
Fig. 4 is the original photo of 8 weeks before using embodiment 2 compositions and after use,
Fig. 5 is the 3D photo before using embodiment 2 compositions,
Fig. 6 is the 3D photo after using embodiment 2 compositions.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described:
Embodiment 1:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.0kg, molecule
Amount 5.4kDa；Hyaluronate sodium I 2.0kg, molecular weight 480kDa；Hyaluronate sodium II 2.0kg, molecular weight 1050kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Embodiment 2:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.2kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 1.8kg, molecular weight 320kDa；Hyaluronate sodium II 2.0kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Embodiment 3:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.2kg, molecule
Amount 8.5kDa；Hyaluronate sodium I 2.0kg, molecular weight 230kDa；Hyaluronate sodium II 1.8kg, molecular weight 1500kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Embodiment 4:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.2kg, molecule
Amount 3.5kDa；Hyaluronate sodium I 1.9kg, molecular weight 120kDa；Hyaluronate sodium II 1.9kg, molecular weight 1010kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Embodiment 5:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.1kg, molecule
Amount 9.5kDa；Hyaluronate sodium I 1.9kg, molecular weight 580kDa；Hyaluronate sodium II 2.0kg, molecular weight 1760kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 1:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 2.0kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 2.0kg, molecular weight 320kDa；Hyaluronate sodium II 6.0kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 2:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 8.0kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 1.0kg, molecular weight 320kDa；Hyaluronate sodium II 1.0kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 3:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 5.0kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 2.5kg, molecular weight 320kDa；Hyaluronate sodium II 2.5kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 4
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 5.8kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 2.1kg, molecular weight 320kDa；Hyaluronate sodium II 2.1kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 5:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.7kg, molecule
Amount 7.0kDa；Hyaluronate sodium I 1.6kg, molecular weight 320kDa；Hyaluronate sodium II 1.7kg, molecular weight 1100kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop
Comparative example 6:
Accurate weighing hydrolysis hyaluronate sodium (enzyme action oligomerization hyaluronate sodium, trade nameHA) 6.0kg, molecule
Amount 8.5kDa；Hyaluronate sodium I 2.0kg, molecular weight 900kDa；Hyaluronate sodium II 2.0kg, molecular weight 2000kDa；Throw respectively
Enter double conic rotary vacuum dryer, open machine, rotate mixing 1 hour.Product after mixing loads sterilized packaging material
Interior (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Comparative example 7:
Accurate weighing hyaluronate sodium I 5.0kg, molecular weight 240kDa；Hyaluronate sodium II 5.0kg, molecular weight
1150kDa；Put into double conic rotary vacuum dryer respectively, open machine, rotate mixing 1 hour.Product after mixing has loaded
In the packaging material of sterilizing (bottled, packed, plastic-aluminum dress etc.).More than operation is all carried out at dust proof workshop.
Test example 1: the moisture-keeping efficacy of hyaluronic acid compositions
With cosmetic emulsion basic components as matched group, add the sample of 0.2% hyaluronic acid compositions as experiment
Group, smears rear skin moisture content by mensuration and compares the difference of sample room moistening effect with skin water loss amount.
1, emulsion basic components preparation technology
Table 1 emulsion basic components
Note: A phase is oil phase, B phase is aqueous phase
1) first A is added to oil phase tank, is heated to 70-75 DEG C, stirs standby to melting completely.
2) B is added to emulsion tank, is warming up to 80-85 DEG C, is uniformly dispersed with 78, adds in tank, insulation, and stirring is to complete
CL.
3) A is added to B phase, opens homogenizing 2 minutes after mixing.It is subsequently adding C phase, stirs.It is cooled between 40-45 DEG C
Add D phase, mix homogeneously, 38-40 DEG C of discharging.
2, test method:
Subject age is 22-55 year, totally 12 people.At right-hand man inner forearm labelling 4 × 4cm2Pilot region, by 3mg
Sample/cm2Consumption, sample is spread evenly across in trial zone.Use moisture of skin tester respectively
CorneometerCM825 (Courage+Khazaka company, Germany) and skin water loss tester Tewanmeter
TM300 (Courage+Khazaka company, Germany) measures and smears front and smear tested region and blank when rear 1h, 2h, 4h, 6h
The moisture content of skin of control zone and skin water loss amount.The test of same volunteer is completed by same survey crew.
When front and back measuring, measurement site keeps consistent with indoor temperature and humidity.
3, computational methods:
4, test result:
Human body skin moisture measurement result is as depicted in figs. 1 and 2.
Knowable to Fig. 1, Fig. 2, each embodiment group skin moisture content significantly improves than comparative example group, water loss amount situation
Also it is obviously improved.Each embodiment is compared, and embodiment 1～2 is better than embodiment 3～5.Therefore, hyaluronic acid compositions can be short
Improving the moisture of skin in time, skin barrier also can maintain preferable state simultaneously, water conservation moisturizing effective.
Test example 2: hyaluronic acid compositions radical scavenging activity is studied
Respectively precision measure 0.1mM DPPH (2-methyl-2,3-dihydro-5,6-diphenyl pyrazine) ethanol solution 5.0mL and
0.5% each embodiment and comparative sample solution 5.0mL put in tool plug test tube, mixing.Mix with isopyknic water and 95% ethanol
Conjunction solution is blank.Room temperature is placed 30 minutes, measures solution absorbance value at 523nm respectively.Free radical scavenger
Ability is the strongest, and absorbance is the least.
The experimental result of the Scavenging ability of hyaluronic acid compositions is shown in Table 2.
The Scavenging ability of table 2 hyaluronic acid compositions
The free radical scavenging activity of embodiment 1,2,4 is apparently higher than each comparative example, and therefore, hyaluronic acid compositions has preferably
The ability removing free radical, thus skin is played and antioxidative effect, delay skin aging.
Test example 3: the hyaluronic acid compositions impact on skin elasticity
Embodiment sample and comparative sample is prepared according to the method in test example 1.
Skin elasticity method of testing: each sample subjects recruitment 10 people, sample spreads upon at Face and cheek, is massaged into gently
All absorb.Every day is the most each once, continues 8 weeks.Respectively at smearing front and smearing rear mensuration by skin elasticity for 2 weeks, 4 weeks, 8 weeks
Instrument MPA580 (Courage+Khazaka company, Germany) measures the Flexible change situation at buccal position (Fructus Mali pumilae flesh).Test two
Secondary, average.
Conventional elasticity indexes has following three kinds:
Thick elastic: R2=Ua/Uf；
Clean elastic: R5=Ur/Ue；
Bioelastic: R7=Ur/Uf；
Result: after smearing 8 weeks, the measurement result of skin elasticity is shown in Table 3.
Table 3 composition containing hyaluronic acid smear 8 weeks after impact on skin elasticity
From table 3 it can be seen that compared with comparative example, after using 8 weeks continuously, embodiment of the present invention sample can significantly improve
The elasticity of skin, recovers skin elasticity.
Test example 4: the hyaluronic acid compositions impact on wrinkle of skin
Embodiment 2 sample, comparative example 4 and comparative example 5 sample is prepared according to the method in test example 1.
Method of testing: each sample subjects recruitment 10 people, sample spreads upon at canthus, and every day is the most each once, continues 8
Week.Within 2 weeks the most after a procedure, 4 weeks, 8 weeks, with human body skin rapid three dimensional imaging system (Primos), is wrinkled in experimenter's right eye angle
The stricture of vagina degree of depth is estimated.
Evaluation methodology:
A smears before and after's photo by shooting and is evaluated
Wrinkle depth is evaluated by b three-dimensional quick imaging system
From figure 3, it can be seen that compared with comparative example, after using 8 weeks continuously, use the tested of the embodiment of the present invention 2 sample
Person, its canthus wrinkle degree of depth substantially alleviates.Fig. 4 is wherein an experimenter canthus photo using embodiment 2 sample, use 8
Zhou Hou, from original photo, its wrinkle improves situation naked eyes, can be seen that wrinkle gully substantially subtracts from 3D photo (Fig. 5,6)
Few.
Therefore, comprehensive above Efficacy experiments data are it can be shown that hyaluronic acid compositions of the present invention takes full advantage of difference
The different skin care effect of molecular weight section HA, by the scavenging action to free radical and many to moisture of skin, elasticity and wrinkle etc.
The improvement of aspect, thus show significant anti-aging effects.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not limited by embodiment
System, the change made, modifies, combines, substitutes, simplifies and all should be under other any spirit without departing from the present invention and principle
Equivalence substitute mode, within being included in protection scope of the present invention.
Claims (9)
1. the anti-ageing compositions of hyaluronic acid, it is characterised in that raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 60% ~ 62% of 3 ~ 8 kDa, and molecular weight is the hyaluronic acid of 300 ~ 500 kDa
Or its salt 18% ~ 20%, molecular weight is hyaluronic acid or its salt 18% ~ 20% of 1000～1200 kDa.
The anti-ageing compositions of hyaluronic acid the most according to claim 1, it is characterised in that raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 60% ~ 62% of 7 kDa, and molecular weight is hyaluronic acid or its salt of 320 kDa
18% ~ 20%, molecular weight is hyaluronic acid or its salt 18% ~ 20% of 1100 kDa.
The anti-ageing compositions of hyaluronic acid the most according to claim 1, it is characterised in that raw material weight percentage composition is as follows:
Molecular weight is hydrolysis hyaluronic acid or its salt 62% of 7 kDa, and molecular weight is hyaluronic acid or its salt 18% of 320 kDa,
Molecular weight is hyaluronic acid or its salt 20% of 1100 kDa.
The anti-ageing compositions of hyaluronic acid the most according to claim 1, it is characterised in that described hydrolysis hyaluronic acid or its
Salt be the bacillus cereus hyaluronidase that obtains with bacillus cereus A50CGMCC NO.5744 fermentation culture to hyaluronic acid or its
Salt carries out degrade the enzyme action oligomerization hyaluronic acid obtained or its salt.
The anti-ageing compositions of hyaluronic acid the most according to claim 1, it is characterised in that described hyaluronate or hydrolysis
Hyaluronate is hyaluronic acid or the hydrolysis sodium salt of hyaluronic acid, potassium salt, magnesium salt, calcium salt or zinc salt.
6. the preparation method of the anti-ageing compositions of hyaluronic acid according to any one of a claim 1 ~ 5, it is characterised in that will
The hyaluronic acid of two kinds of molecular weight or its salt mix according to weight ratio with hydrolysis hyaluronic acid or its salt, stir, to obtain final product.
7. the anti-ageing compositions of hyaluronic acid according to any one of a claim 1 ~ 5 or the preparation described in claim 6
The anti-ageing compositions of hyaluronic acid that method obtains application in preparing anti-aging product.
Application the most according to claim 7, it is characterised in that described defying age be moisturizing, remove free radical, cytothesis,
Recover skin elasticity.
Application the most according to claim 7, it is characterised in that described anti-aging product is toner/smoothing toner, essence, curry, breast
Liquid, cream, facial film, color make-up, fancy soap, cleansing milk or bath gel.
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| CN114748375A (en) * | 2022-04-27 | 2022-07-15 | 康伦智美科技产业(青岛)有限公司 | Skin care essence containing high-concentration ultra-small molecule sodium hyaluronate and preparation method thereof |
| CN115746168A (en) * | 2022-11-15 | 2023-03-07 | 华熙生物科技股份有限公司 | Potassium hyaluronate and application thereof |
| CN115836987A (en) * | 2022-12-23 | 2023-03-24 | 广州市飞科贸易有限公司 | Anti-aging high-permeability hyaluronic acid composition and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101123942A (en) * | 2005-01-03 | 2008-02-13 | 诺维信生物聚合物公司 | Hyaluronic acid fraction with moisturizing and anti-wrinkle properties |
| CN101437527A (en) * | 2006-05-11 | 2009-05-20 | 桑德拉·戈博 | Hyaluronic acid binary mixture and treatment purpose thereof |
| CN101500535A (en) * | 2006-06-28 | 2009-08-05 | 诺维信生物聚合物公司 | Compositions with several hyaluronic acid fractions for cosmetic and medical uses |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN105233270A (en) * | 2015-11-12 | 2016-01-13 | 上海昊海生物科技股份有限公司 | Medicine composition for water-optical treatment and application of medicine composition |
-
2016
- 2016-08-02 CN CN201610629387.6A patent/CN106137786B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101123942A (en) * | 2005-01-03 | 2008-02-13 | 诺维信生物聚合物公司 | Hyaluronic acid fraction with moisturizing and anti-wrinkle properties |
| CN101437527A (en) * | 2006-05-11 | 2009-05-20 | 桑德拉·戈博 | Hyaluronic acid binary mixture and treatment purpose thereof |
| CN101500535A (en) * | 2006-06-28 | 2009-08-05 | 诺维信生物聚合物公司 | Compositions with several hyaluronic acid fractions for cosmetic and medical uses |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN105233270A (en) * | 2015-11-12 | 2016-01-13 | 上海昊海生物科技股份有限公司 | Medicine composition for water-optical treatment and application of medicine composition |
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| CN111588652A (en) * | 2020-05-14 | 2020-08-28 | 华熙生物科技股份有限公司 | Composition containing hyaluronic acid and polyalcohol and application thereof |
| CN114191331A (en) * | 2021-12-10 | 2022-03-18 | 华熙生物科技股份有限公司 | Anti-skin-aging composition, application thereof and anti-skin-aging product |
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| CN114748375A (en) * | 2022-04-27 | 2022-07-15 | 康伦智美科技产业(青岛)有限公司 | Skin care essence containing high-concentration ultra-small molecule sodium hyaluronate and preparation method thereof |
| CN115746168A (en) * | 2022-11-15 | 2023-03-07 | 华熙生物科技股份有限公司 | Potassium hyaluronate and application thereof |
| CN115836987A (en) * | 2022-12-23 | 2023-03-24 | 广州市飞科贸易有限公司 | Anti-aging high-permeability hyaluronic acid composition and preparation method thereof |
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