CN106119209B - 一种抗肠道病毒EV71 IgA单克隆抗体及应用 - Google Patents
一种抗肠道病毒EV71 IgA单克隆抗体及应用 Download PDFInfo
- Publication number
- CN106119209B CN106119209B CN201610506079.4A CN201610506079A CN106119209B CN 106119209 B CN106119209 B CN 106119209B CN 201610506079 A CN201610506079 A CN 201610506079A CN 106119209 B CN106119209 B CN 106119209B
- Authority
- CN
- China
- Prior art keywords
- antibody
- iga
- monoclonal antibody
- virus
- igg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 47
- 241001529459 Enterovirus A71 Species 0.000 claims abstract description 36
- 238000001514 detection method Methods 0.000 claims abstract description 29
- 230000028327 secretion Effects 0.000 claims description 11
- 201000010099 disease Diseases 0.000 claims description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- 229940079593 drug Drugs 0.000 claims description 9
- 239000003814 drug Substances 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000002574 poison Substances 0.000 claims description 5
- 231100000614 poison Toxicity 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 36
- 241000700605 Viruses Species 0.000 abstract description 18
- 238000002965 ELISA Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000001413 cellular effect Effects 0.000 abstract description 3
- 231100000572 poisoning Toxicity 0.000 abstract description 2
- 230000000607 poisoning effect Effects 0.000 abstract description 2
- 101710132601 Capsid protein Proteins 0.000 description 17
- 101710197658 Capsid protein VP1 Proteins 0.000 description 17
- 101710118046 RNA-directed RNA polymerase Proteins 0.000 description 17
- 101710108545 Viral protein 1 Proteins 0.000 description 17
- 208000015181 infectious disease Diseases 0.000 description 17
- 238000000034 method Methods 0.000 description 13
- 230000003612 virological effect Effects 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- 206010003445 Ascites Diseases 0.000 description 8
- 238000003118 sandwich ELISA Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000009385 viral infection Effects 0.000 description 7
- 241000709661 Enterovirus Species 0.000 description 6
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- 239000013642 negative control Substances 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000002372 labelling Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000011725 BALB/c mouse Methods 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108010040721 Flagellin Proteins 0.000 description 4
- 208000020061 Hand, Foot and Mouth Disease Diseases 0.000 description 4
- 208000025713 Hand-foot-and-mouth disease Diseases 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 238000007912 intraperitoneal administration Methods 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 238000006386 neutralization reaction Methods 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 230000000840 anti-viral effect Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 210000000234 capsid Anatomy 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000011895 specific detection Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 108090000565 Capsid Proteins Proteins 0.000 description 2
- 102100023321 Ceruloplasmin Human genes 0.000 description 2
- 101900263680 Measles virus Matrix protein Proteins 0.000 description 2
- 108090001074 Nucleocapsid Proteins Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 241000709664 Picornaviridae Species 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 102000008234 Toll-like receptor 5 Human genes 0.000 description 2
- 108010060812 Toll-like receptor 5 Proteins 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000011091 antibody purification Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229940031551 inactivated vaccine Drugs 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 210000000605 viral structure Anatomy 0.000 description 2
- 210000002845 virion Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 208000006740 Aseptic Meningitis Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000709687 Coxsackievirus Species 0.000 description 1
- 241000539679 Defiwi virus Species 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- OWIKHYCFFJSOEH-UHFFFAOYSA-N Isocyanic acid Chemical compound N=C=O OWIKHYCFFJSOEH-UHFFFAOYSA-N 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 206010027201 Meningitis aseptic Diseases 0.000 description 1
- 208000009525 Myocarditis Diseases 0.000 description 1
- 101150095279 PIGR gene Proteins 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 206010037423 Pulmonary oedema Diseases 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 101150024766 VP1 gene Proteins 0.000 description 1
- 108010087302 Viral Structural Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- XLJMAIOERFSOGZ-UHFFFAOYSA-N anhydrous cyanic acid Natural products OC#N XLJMAIOERFSOGZ-UHFFFAOYSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 150000004965 peroxy acids Chemical class 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 208000005333 pulmonary edema Diseases 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000018448 secretion by cell Effects 0.000 description 1
- 210000002955 secretory cell Anatomy 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1009—Picornaviridae, e.g. hepatitis A virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610506079.4A CN106119209B (zh) | 2016-07-01 | 2016-07-01 | 一种抗肠道病毒EV71 IgA单克隆抗体及应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610506079.4A CN106119209B (zh) | 2016-07-01 | 2016-07-01 | 一种抗肠道病毒EV71 IgA单克隆抗体及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106119209A CN106119209A (zh) | 2016-11-16 |
CN106119209B true CN106119209B (zh) | 2019-10-25 |
Family
ID=57468647
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610506079.4A Active CN106119209B (zh) | 2016-07-01 | 2016-07-01 | 一种抗肠道病毒EV71 IgA单克隆抗体及应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106119209B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110551211B (zh) * | 2018-05-30 | 2022-05-24 | 福又达生物科技股份有限公司 | 含抗肠病毒71型vp1蛋白单克隆抗体的检测试剂盒 |
CN110078821B (zh) * | 2019-03-20 | 2022-03-25 | 天津大学 | 肠道病毒d组68型vp1单克隆抗体的序列及其应用 |
CN114045291B (zh) * | 2021-11-01 | 2022-07-19 | 武汉爱博泰克生物科技有限公司 | 重组人Sema4C蛋白、表达载体、宿主细胞以及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102409028A (zh) * | 2011-11-03 | 2012-04-11 | 北京科兴生物制品有限公司 | 肠道病毒71型单克隆抗体及其应用 |
WO2015092668A1 (en) * | 2013-12-16 | 2015-06-25 | Mab Explorations Sdn Bhd | Antibodies specific for enteroviruses that infect humans |
CN105567643A (zh) * | 2015-12-28 | 2016-05-11 | 菲鹏生物股份有限公司 | 可分泌抗ev71病毒蛋白vp1单克隆抗体的杂交瘤细胞、单克隆抗体及应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9051361B2 (en) * | 2010-08-17 | 2015-06-09 | National Health Research Institutes | Immunogenic compositions and uses thereof |
-
2016
- 2016-07-01 CN CN201610506079.4A patent/CN106119209B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102409028A (zh) * | 2011-11-03 | 2012-04-11 | 北京科兴生物制品有限公司 | 肠道病毒71型单克隆抗体及其应用 |
WO2015092668A1 (en) * | 2013-12-16 | 2015-06-25 | Mab Explorations Sdn Bhd | Antibodies specific for enteroviruses that infect humans |
CN105567643A (zh) * | 2015-12-28 | 2016-05-11 | 菲鹏生物股份有限公司 | 可分泌抗ev71病毒蛋白vp1单克隆抗体的杂交瘤细胞、单克隆抗体及应用 |
Also Published As
Publication number | Publication date |
---|---|
CN106119209A (zh) | 2016-11-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103214570B (zh) | 特异于流感病毒h5亚型或者n1亚型的血凝素和神经氨酸酶的单克隆抗体及其应用 | |
Berry et al. | Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus | |
KR101785290B1 (ko) | 중증 열성 혈소판 감소 증후군 바이러스 감염 진단용 단클론항체, 이를 생산하는 하이브리도마 및 이 단클론항체를 사용하는 중증 열성 혈소판 감소 증후군 바이러스 감염 진단방법 | |
WO2007026689A1 (ja) | ダチョウを用いた抗体、及びその作製方法 | |
CN103266088B (zh) | 一种h7亚型禽流感病毒单克隆抗体及试剂盒 | |
CN105675873B (zh) | 一种检测试剂盒及其应用 | |
CN106119209B (zh) | 一种抗肠道病毒EV71 IgA单克隆抗体及应用 | |
CN101643721B (zh) | 广谱安全型动物用抗甲型流感病毒疫苗 | |
CN103059132B (zh) | 抗h9亚型流感病毒血凝素蛋白的单克隆抗体及其应用 | |
CN109142724B (zh) | 一种用于检测i群禽腺病毒4型抗体的阻断elisa试剂盒及其应用 | |
Dong et al. | Analysis of epitopes in the capsid protein of avian hepatitis E virus by using monoclonal antibodies | |
Kobayashi et al. | Characterization and epitope mapping of monoclonal antibodies raised against rat hepatitis E virus capsid protein: An evaluation of their neutralizing activity in a cell culture system | |
CN101003572A (zh) | 牙鲆抗淋巴囊肿病毒免疫球蛋白的单克隆抗体及其制备方法 | |
Zhang et al. | Characterization of monoclonal antibodies against duck hepatitis type 1 virus VP1 protein | |
CN105504051B (zh) | 狂犬病毒核蛋白单克隆抗体及其应用 | |
CN107557344A (zh) | 一株表达猪瘟病毒e2蛋白单克隆抗体杂交瘤细胞系csfv‑3h3g6及抗体与试剂盒 | |
CN108918875A (zh) | 鸽新城疫病毒单克隆抗体及在制备诊断和检测试剂盒中的应用 | |
CN114316037B (zh) | 一种O型口蹄疫病毒结构蛋白的抗体m19、制备方法及应用 | |
CN109851671A (zh) | 检测口蹄疫o型广西株抗原的单克隆抗体及其应用 | |
CN102533664A (zh) | 分泌抗水稻黑条矮缩病毒单抗杂交瘤细胞株及其单抗应用 | |
CN105198969A (zh) | 一种1型鸭甲肝病毒vp3蛋白的b细胞表位及其鉴定方法和应用 | |
CN103421112B (zh) | 一种抗肠道病毒的结合分子及其用途 | |
CN105925537B (zh) | 一种抗HSV的糖蛋白gD的单克隆抗体以及产生该抗体的杂交瘤细胞 | |
Fayaz et al. | Development and characterization of mouse monoclonal antibodies to canine morbillivirus | |
CN110144329B (zh) | 杂交瘤细胞株6b1及其分泌的抗口蹄疫a型病毒的单克隆抗体与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A monoclonal antibody against enterovirus EV71 IgA and its application Effective date of registration: 20201126 Granted publication date: 20191025 Pledgee: Bank of Communications Co.,Ltd. Wuhan Branch of Hubei Free Trade Experimental Zone Pledgor: WUHAN ABCLONAL Inc. Registration number: Y2020420000080 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231227 Granted publication date: 20191025 Pledgee: Bank of Communications Co.,Ltd. Wuhan Branch of Hubei Free Trade Experimental Zone Pledgor: WUHAN ABCLONAL Inc. Registration number: Y2020420000080 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |