CN106109270B - A kind of anti-aging cosmetics composition and preparation method thereof - Google Patents

Abstract

The purpose of the present invention is to provide a kind of new cosmetic compositions with senile-resistant efficacy, the composition can effectively eliminate free radical, reduce wrinkle of skin generation, to reach delay skin aging effect.The composition composition is as follows: phenethyl caffeate, propylene glycol, glycerol, 1,3-BDO, and 1,2- pentanediol, the different monooctyl ester of ferulic acid, xanthan gum, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, emulsifier, cetostearyl alcohol, polymethyl siloxane, vegetable oil, freshener, VB₅, preservative, essence, deionized water refines through simple and easy preparation method.

Description

A kind of anti-aging cosmetics composition and preparation method thereof

Technical field

The present invention relates to cosmetics processing technique fields in daily-use chemical industry, and in particular to a kind of anti-aging cosmetics composition And preparation method thereof.

Technical background

Skin is the maximum organ of human body, as the continuous increase at age can gradual aging.It is reported that most women 25 Start long wrinkle within~30 years old, color spot further occur, subsequent skin becomes loose, coarse, dim, wrinkle is deepened, and becomes mostly etc..Skin The aging of skin generates negative influence, the process of skin aging to work, the life of people almost with everyone lower half a lifetime It is divided into inherent and external two processes.

1. intrinsic procedure

Intrinsic procedure refers to that as time goes by, people is more and more older, will appear some small microgrooves, cutis laxa on skin Deng.The endogenetic mechanisms of skin aging are extremely complex, and more representational causes of senescence specifically includes that free radical theory, loses Pass gene theory, mitochondrial DNA damage theory and other theories.

1) free radical theory

Free radical is also known as free radical, is exactly oxygen radical, is to be metabolized to generate by vivo oxidation.Free radical Say to be Harman in proposition in 1956, its centre point includes: that free radical is prevalent in living organism, is had The active intermediate of elevated chemical.Under normal physiological conditions, the generation of machine interior free yl and eliminates and be in dynamic equilibrium, one Denier balance is destroyed, and will lead to the generation of excessive free radicals；Excessive free radical is to chromosome, mitochondria, cell membrane and connective The murder by poisoning sexual assault of the biological tissues such as tissue can cause the aging of body.

2) theory of genetic gene

Theory of genetic gene mainly analyzes aging phenomenon from chromosome and gene level.The theory thinks that aging is by certain The biological phenomena that kind genetic program is determined and expressed on time.With advancing age, modifier forfeiture, DNA methylation The regulations such as reduction, phosphorylation reaction reduction, telomere shortening, the decline of DNA self-repairing capability, proto-oncogene and tumor suppressor gene are abnormal Chromosome mutation, normal cell is caused excessively to break up and aging performance occur.Hayflik earliest cell injuring model has found Cell passage rule, it is believed that developmental process having time succession, controlling mechanism weaken with age, eventually lead to and decline Always.

3) mitochondrial DNA damage theory

1980, Miquel and Cowiker proposed the mitochondria hypothesis of aging, it is believed that the damage of mitochondrial DNA is thin Born of the same parents' aging and dead molecular basis, its research becomes the hot spot for studying Aging Mechanism in the world in recent years.From cell biological It learns angle to say, the energy 90% that Cellular Oxidation phosphorylation generates works as mitochondrial DNA for the need of cell life from mitochondria When damage, the energy of generation is reduced, and is influenced the energy supply of cell, is led to the decline of cell, tissue, organ dysfunction, therefore recognizes For the deformation of mitochondria, leaks and rupture is all the major reason of its aging.Delay the destructive process of mitochondria, may extend Cell survival, and then extend the service life of body.

4) other theories

Including enzymes activity transformant doctrine, immunological theory, hormone theory, tolemerase, missing of essential trace element etc.. Various suppositions have been done to Aging Mechanism from different level and angle.

2. extrinsic process

Extrinsic process is often referred to exposure influence caused by skin of environment or other forms, caused some variations. Such as deeper thicker wrinkle, decortication, dry skin, senile plaque etc..Extrinsic factor mainly includes ultraviolet light irradiation, cigarette cigarette Mist and other bad life habits etc..The presence of extrinsic factor can accelerate the process of skin natural aging.

How delay senescence one of the research focus of always cosmetic field, the anti-aging makeup occurred currently on the market Product are many kinds of, mostly addition vitamin antioxidants (such as vitamin E, vitamin C), but this kind of antioxidant stability compared with Difference, application are restricted.Scholar, which studies, in recent years finds to contain antioxidant content in some plants or Chinese herbal medicine have confrontation The effect of free radical promotes skin metabolism, delays body aging, but in practical application, it is existing to contain above-mentioned plant or medium-height grass The effect is unsatisfactory for the wrinkle resistant anti-aging effects that the cosmetics of medicament extract play.Therefore, a kind of wrinkle resistant anti-aging effects are studied Well, stability is good, for the pure natural skin care compositions of cosmetics very it is necessary to.

Summary of the invention

The purpose of the present invention is to provide a kind of cosmetic composition with senile-resistant efficacy, the composition can effectively disappear Except free radical, wrinkle of skin generation is reduced, to reach delay skin aging effect.

Phenethyl caffeate is one of main active of propolis, contains O- dihydroxy (catechol) benzene ring structure, tool There is strong inoxidizability, removes free radical, anti-aging effects, whitening function.For remove free radical a kind of common structure, and There are two ortho position phenolic hydroxyl group, keep electron density on phenyl ring high, easily autoxidation and colour generation.The report such as Bors, O- dihydroxy benzenes Ring structure plays an important role performance antioxidation in flavonoids.

The present invention is attained in that by implementer's case in detail below

The present invention provides a kind of anti-aging cosmetics composition, is prepared by the raw material of following parts by weight: caffeic acid benzene 1~30 part of ethyl ester, 1~30 part of propylene glycol, 1~20 part of glycerol, 1~10 part of 1,3-BDO, 1,2- 1~20 part of pentanediol, Ah Different 1~30 part of monooctyl ester of Wei's acid, 1~2 part of xanthan gum, 0.1~0.5g parts of Sodium Hyaluronate, disodium ethylene diamine tetraacetate 0.1~1.5 Part, it is 30~60 parts of emulsifier, 10~30 parts of cetostearyl alcohol, 5~30 parts of polymethyl siloxane, 30~60 parts of vegetable oil, refrigerant 1~20 part of agent, VB₅5~10 parts, 2~10 parts of preservative, 1~5 part of essence, surplus is supplemented to 1000 parts by weight with deionized water.

A kind of anti-aging cosmetics composition provided by the invention, the composition in parts by weight, preferably by following heavy The raw material for measuring part forms, and 15 parts of phenethyl caffeate, 15 parts of propylene glycol, 10 parts of glycerol, 5 parts of 1,3-BDO, 1,2- pentanediol 10 parts, 15 parts of the different monooctyl ester of ferulic acid, 1.5 parts of xanthan gum, 0.3g parts of Sodium Hyaluronate, 0.8 part of disodium ethylene diamine tetraacetate, emulsification 45 parts of agent, 20 parts of cetostearyl alcohol, 20 parts of polymethyl siloxane, 45 parts of vegetable oil, 10 parts of freshener, VB₅8 parts, preservative 6 Part, 3 parts of essence, surplus is supplemented to 1000 parts by weight with deionized water.

A kind of anti-aging cosmetics composition provided by the invention, vegetable oil used are Sweet Almond Oil, avocado oil, the wooden fruit of cream The mixture of oil, group becomes 3~8 parts of Sweet Almond Oil, 3~6 parts of avocado oil, 1~5 part of shea butter in parts by weight.

A kind of anti-aging cosmetics composition provided by the invention, freshener used are menthol.

The present invention provides a kind of anti-aging cosmetics composition, and preservative used is Phenoxyethanol.

The preparation method for preparing anti-aging cosmetics composition of the present invention, includes the following steps:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 70~85 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70 ~85 DEG C, keep the temperature 15~30 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, heat preservation for standby use until completely dissolved；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 15~45 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring Uniformly.

It is preferred that preparing the preparation method of anti-aging cosmetics composition of the present invention, include the following steps:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 80 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 80 DEG C, keep the temperature 20 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 78 DEG C, to It is completely dissolved rear heat preservation for standby use；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm After mixing 4 minutes, then with stirring rate be 30rpm stir 30 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring Uniformly.

The main composition of the anti-aging cosmetics composition has:

1. phenethyl caffeate.Phenethyl caffeate is one of main active of propolis, contains O- dihydroxy (catechu Phenol) benzene ring structure, there is strong antioxidation, free radical can be removed, there is anti-aging effects and whitening function, be the combination The main component of object, phenethyl caffeate weight ratio are 1g~30g/1000g.

2. propylene glycol.It is as a kind of wetting agent in the present invention.

3. glycerol.It is as a kind of moisturizer in the present invention.

4. 1,3-BDO.It is one kind of polyalcohol, is used as moisturizer and solvent in the present invention.

5. 1,2- pentanediol.It is used as antibacterial agent in the present invention.

6. the different monooctyl ester of ferulic acid.Have the function of absorb ultraviolet light and prevent oxidation, while also have depigmentation and The function of inhibiting tyrosinase, has inhibiting effect to melanin production.

7. xanthan gum.A kind of emulsifier is used as in the present invention.

8. Sodium Hyaluronate.With efficient water lock moisture-keeping function and skin repair group, compact skin, permanent moisturizing, It prevents from being dehydrated, promotes histocyte regeneration.

9. disodium ethylene diamine tetraacetate.A kind of complexing agent is used as in the present invention.

10. cetostearyl alcohol.Cetostearyl alcohol of the present invention is the mixture of the pure and mild C18 alcohol of C16, preferred C16 Alcohol: when the mass ratio of C18 alcohol is 3: 7, system stability can be made to increase.Its source is by cottonseed oil, palm oil through transesterification, High pressure oxygenation is restored and is made.It plays thick stabilization in this formula.

11. polymethyl siloxane.A kind of water-repellent additive is used as in the present invention.

Emulsifier of the present invention is Tween-80, and hydrolecithin, lecithin, stearine, PEG-100 are stearic Acid esters, paregal O, one of lauryl sodium sulfate or their mixing.It is further preferred that the emulsifier is PEG- 100 stearates, paregal O, the weight ratio are 30~60g/1000g.

Vegetable oil of the present invention is Sweet Almond Oil, avocado oil, shea butter, olive oil, jojoba oil, grape pip One of oil, macadimia nut oil or their mixing.It is further preferred that the vegetable oil is Sweet Almond Oil, avocado oil, cream Wooden fruit oil, the Sweet Almond Oil, avocado oil, shea butter weight ratio ratio be respectively 30~80g/1000g: 30~60g/ 1000g: 10~50g/1000g.Sweet Almond Oil is derived from the peaberry of sweet tea apricot, is unlikely to deteriorate, and has excellent keeping quality, to skin without Evil, there is moisturizing effect；Avocado oil is derived from the pulp of avocado, containing more rich micro constitutent, such as tocopherol, various plant steroids Alcohol, phytin, carotenoid and squalene etc..Avocado oil is widely used as the lubricant of cosmetics to promote skin It is smooth, it is stronger to percutaneous permeability, help to be conveyed into active material in skin, furthermore it there are also anti-sunlight functions；Newborn wood fruit The extraction of oil is originated from missible oil wood, is suitable for anti-aging product, the care product of late frost, sensibility skin and dry skin.

The advantageous effects that the present invention generates are:

1. phenethyl caffeate is main component of the invention, there is strong inoxidizability, whitening and anti-aging effect.

2. Multiple components are mixed in a certain ratio preparation by the compounds of this invention, for the mechanism of human senility, from multiple Aspect prevents human senility, and endlessly power can be provided for anti-aging.

3. vegetable oil selects Sweet Almond Oil, avocado oil, shea butter to be mixed in a certain proportion use, can preferably play anti- Aging effect, moisturizes the skin.

4. preparation method is simple by the present invention, energy conservation and environmental protection.

Specific embodiment

Embodiment 1

Prepare a kind of anti-aging cosmetics according to preparation method provided by the invention, including component it is as follows:

1~30 part of phenethyl caffeate, 1~30 part of propylene glycol, 1~20 part of glycerol, 1~10 part of 1,3-BDO, 1,2- 1~20 part of pentanediol, 1~30 part of the different monooctyl ester of ferulic acid, 1~2 part of xanthan gum, 0.1~0.5g parts of Sodium Hyaluronate, ethylenediamine tetraacetic 0.1~1.5 part of acetic acid disodium, 30~60 parts of emulsifier, 10~30 parts of cetostearyl alcohol, 5~30 parts of polymethyl siloxane, plant 30~60 parts of oil, 1~20 part of freshener, VB₅5~10 parts, 2~10 parts of preservative, 1~5 part of essence, surplus is mended with deionized water It is charged to 1000 parts.

It is specific the preparation method is as follows:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 70~85 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70 ~85 DEG C, keep the temperature 15~30 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, heat preservation for standby use until completely dissolved；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 15~45 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring Uniformly.

Embodiment 2

Prepare a kind of anti-aging cosmetics according to preparation method provided by the invention, including component it is as follows:

Phenethyl caffeate 1g, propylene glycol 30g, glycerol 20g, 1,3-BDO 1g, 1,2- pentanediol 1g, ferulic acid are different pungent Ester 30g, xanthan gum 2g, Sodium Hyaluronate 0.1g, disodium ethylene diamine tetraacetate 1.5g, emulsifier 30g, cetostearyl alcohol 30g gather Methylsiloxane 5g, vegetable oil 30g, freshener 1g, VB₅5g, preservative 10g, essence 1g, surplus are supplemented to deionized water 1000g。

It is specific the preparation method is as follows:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 70 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70 DEG C, keep the temperature 15 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70 DEG C, to It is completely dissolved rear heat preservation for standby use；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 15 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring Uniformly.

Embodiment 3

Prepare a kind of anti-aging cosmetics according to preparation method provided by the invention, including component it is as follows:

Phenethyl caffeate 30g, propylene glycol 1g, glycerol 1g, 1,3-BDO 10g, 1,2- pentanediol 20g, ferulic acid are different Monooctyl ester 1g, xanthan gum 1g, Sodium Hyaluronate 0.5g, disodium ethylene diamine tetraacetate 0.1g, emulsifier 60g, cetostearyl alcohol 10g, Polymethyl siloxane 30g, 30~60g of vegetable oil, freshener 20g, VB₅10g, preservative 2g, essence 5g, surplus deionization Water is supplemented to 1000g.

It is specific the preparation method is as follows:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 85 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 85 DEG C, keep the temperature 30 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, heat preservation for standby use until completely dissolved；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 45 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring Uniformly.

Embodiment 4

Prepare a kind of anti-aging cosmetics according to preparation method provided by the invention, including component it is as follows:

Phenethyl caffeate 15g, propylene glycol 15g, glycerol 10g, 1,3-BDO 5g, 1,2- pentanediol 10g, ferulic acid are different Monooctyl ester 15g, xanthan gum 1.5g, Sodium Hyaluronate 0.3g, disodium ethylene diamine tetraacetate 0.8g, emulsifier 45g, cetostearyl alcohol 20g, polymethyl siloxane 20g, vegetable oil 45g, freshener 10g, VB₅8g, preservative 6g, essence 3g, surplus deionized water It is supplemented to 1000g.

It is specific the preparation method is as follows:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 70~85 DEG C, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70 ~85 DEG C, keep the temperature 15~30 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, heat preservation for standby use until completely dissolved；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 15~45 minutes；

Lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring is equal It is even.

Embodiment 5

Prepare a kind of anti-aging cosmetics according to preparation method provided by the invention, including component it is as follows:

Phenethyl caffeate 22g, propylene glycol 17g, glycerol 12g, 1,3-BDO 7g, 1,2- pentanediol 15g, ferulic acid are different Monooctyl ester 16g, xanthan gum 1g, Sodium Hyaluronate 0.4g, disodium ethylene diamine tetraacetate 1g, emulsifier 50g, cetostearyl alcohol 25g gather Methylsiloxane 25g, vegetable oil 40g, freshener 15g, VB₅5g, preservative 10g, essence 4g, surplus are supplemented with deionized water To 1000g.

It is specific the preparation method is as follows:

E) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase Pot, is heated to 70~85 DEG C, heat preservation for standby use after waiting all components to dissolve；

F) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70 ~85 DEG C, keep the temperature 15~30 minutes；

G) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, heat preservation for standby use until completely dissolved；

H) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is stirred with stirring rate for 3000rpm Mix 3~after five minutes, then with stirring rate be that 30rpm is stirred 15~45 minutes；

Lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stirring is equal It is even.

The antioxidant effect of the investigation of experimental example 1 embodiment 2-5

Experiment purpose: compared with being purged the measurement of free radical ability with commercially available common antidotal agent.

Test apparatus and reagent: spectrophotometer SpectronicGENESYS-5, Thermo company, the U.S.；Other reagents For conventional reagent；Compare the oxidation resistance of 2-5 of embodiment of the present invention anti-apolexis composition, Microbial biomass C, vitamin E.

Test method

(1) measurement of scavenging ability of DPPH free radical: successively by pH6.8 phosphate buffer 3mL, 0.4mmol/LDPPH without Hydrous ethanol solution 4mL, 0.2mL sample to be tested, which is added in 10mL centrifuge tube, to be mixed well, and after 30min, is centrifuged 10min (8000r/min), takes supernatant, is the place 516nm measurement absorbance value in wavelength, is averaged for operation repetitive 3 times, laboratory sample To clearance rate=[1- (A1-A2)/A3] of DPPH free radical.A1 is the absorbance value of DPPH solution after adding example reaction in formula, A2 is the light absorption value that DPPH (being replaced with dehydrated alcohol) is not added and only adds sample solution, and A3 is that sample is not added only to add DPPH and aqueous solution Light absorption value.

(2) measurement of ultra-oxygen anion free radical ability: the buffer system (Tris-HCl- that secure ph is 8.20 is removed EDTA).Sample to be tested 0.6mL is added in 10mL centrifuge tube, 5.2mL buffer is added, 0.045mol/L is added with pipette Pyrogallol solution 0.2mL, shakes up rapidly, and waiting reaction after five minutes, rapidly in drying in cuvette, is at 325nm in wavelength Absorbance value is surveyed, A0 is denoted as, is surveyed again after 10min and be denoted as A4.With distilled water replace sample measurement light absorption value be denoted as respectively B0 and B4.It is averaged for operation repetitive 3 times, inhibiting rate=[(B4-B0)-(A4-A0)]/(B4-B0) of the sample to superoxide anion.

(3) measurement of scavenging hydroxyl ability: respectively by 0.4mmol/L crystal violet 1mL, 0.2mol/LpH7.4 phosphoric acid Buffer 3mL, 0.001mol/L FeSO₄Solution 1mL, sample to be tested 1mL are added in 10mL centrifuge tube, finally plus 30% H₂O₂1mL starting reaction, 12000r/min is centrifuged 5min after reacting 1h at room temperature, and supernatant is taken to measure light absorption value at 510nm It is denoted as C, light absorption value when sample is replaced to be denoted as C1 with distilled water, replaces sample and H with distilled water₂O₂When light absorption value be denoted as C2. It is averaged for operation repetitive 3 times, clearance rate=(C-C1)/(C2-C1) of the sample to hydroxy radical.

Test result

The measurement result of 1 scavenging ability of DPPH free radical of table

Note: compared with vitamin C,^*P < 0.05,^**P < 0.01；Compared with vitamin E,^△P < 0.05,^△△P < 0.01.

The result shows that: the ability that the present invention removes DPPH free radical is substantially better than vitamin C and vitamin E.

The measurement result of the removing ultra-oxygen anion free radical ability of table 2

Note: compared with vitamin C,^*P < 0.05,^**P < 0.01；Compared with vitamin E,^△P < 0.05,^△△P < 0.01.

The result shows that: the ability that the present invention removes ultra-oxygen anion free radical is substantially better than vitamin C and vitamin E.

The measurement result of 3 scavenging hydroxyl ability of table

The result shows that: the ability of scavenging hydroxyl of the present invention is substantially better than vitamin C and vitamin E.

From the mechanism of skin aging it is found that skin aging weaken with internal antioxidative functional, free radical increase it is related.Freely Base can cause to damage to skin from many aspects, to accelerate the aging of skin, therefore, whether anti-aging product has anti-aging Effect can determine the removing of free radical, inhibitory effect by it.Free radical it is many kinds of, anti-aging product should have comprehensively Remove the effect of free radical.As table 1, table 2, table 3 it is found that anti-apolexis composition described in 2-5 of the embodiment of the present invention all has relatively by force Removing free radical and oxidation resistance.

2 human skin irritation test of experimental example

Test objective: the safety of anti-apolexis composition of the present invention is detected.

Test method: the closed patch test of 48h is used.It is dilute that 2-5 anti-apolexis composition of the embodiment of the present invention is weighed respectively Aqueous solution 0.020mL is released into spot reagent as experimental group, while blank control group is set.Healthy volunteer 40 are chosen, year Age 18-30, no allergies.It, will be added with the non-stimulated glue of the spot tester of anti-apolexis composition of the present invention after cleaning subject back Band is pasted on back selected location, is gently pressed after pasting with finger and is allowed to uniformly be affixed on skin, continues 48h.It enjoins tested It keeps patch position dry in person 48h, avoids vigorous exercise, scratches and grab spot examination position, long-time solar radiation etc..It is removed after 48h Examination device simultaneously marks, and waits impressions to disappear under sufficient light after 30min and is determined, and is returned in 72h and 96h respectively Visit observation.

4 skin adverse reaction grading standard of table

Experimental result:

Irritation of 5 present invention of table to skin

The result shows that 40 subjects are negative reaction, the present composition is prompted not have adverse reaction.

3 senile-resistant efficacy evaluation test of experimental example

Test objective: for the senile-resistant efficacy for verifying the invention anti-apolexis composition, human trial is carried out.

Test apparatus: spectrophotometer SpectronicGENESYS-5, skin elasticity tester Cutometer MPA.

Test principle: healthy volunteer 40, age 25-40 are chosen, no allergies.

Prepare before test: subject being randomly divided into 4 groups, every group of subject's left arm smears the addition embodiment of the present invention respectively 2-5 anti-senile preparation, right forearm smear the frost for not adding anti-ageing ingredient, use as control.

Smearing method: subject is used continuously the present composition 30 days, and the left forearm crook of the arm is applied to after washing one's face and rinsing one's mouth sooner or later daily Place.Applying amount is to be slightly shown in smear layer.The frost for not adding anti-ageing ingredient is smeared at the right forearm crook of the arm, is used as control.

Test position: specimen locations are smeared at the left and right forearm crook of the arm of subject, follow-on test 6 times, are averaged.

Testing time: it is surveyed using 1 day (being denoted as 0 day) before tested products, and after continuous use 10 days, 20 days, 30 days Scale skin renewal rate, skin elasticity.

Test method:

Skin elasticity measurement: probe vertical is gently pressed on tested skin surface and tested by labeled test position.Elasticity ginseng Count the ability that original position is restored to after representing skin deformation using ratio.For ratio closer to 1, skin elasticity is better.R2: nothing The springback capacity of skin and the ratio between maximum tension amount when having a negative pressure, R5: skin in the first time cyclic process of skin test when negative pressure The elastic part of skin recovery process and the ratio between the elastic part for adding negative pressure process, R7: in the first time cyclic process of skin test The ratio between the maximum tension amount of skin in the elastic part of skin recovery process and current cyclic process.

Test result:

Skin elasticity measurement result: 4 groups of subjects are respectively using addition 2-5 of embodiment of the present invention anti-apolexis composition, Do not occur adverse reaction, test result is as follows:

Table 6 is compared using skin elasticity after the anti-ageing white different time of addition 2 anti-senile preparation of embodiment

Note: with use it is preceding compared with,^*P < 0.5,^**P < 0.01

Table 7 is compared using skin elasticity after the anti-ageing white different time of addition 3 anti-senile preparation of embodiment

Note: with use it is preceding compared with,^*P < 0.5,^**P < 0.01

Table 8 is compared using skin elasticity after the anti-ageing white different time of addition 4 anti-senile preparation of embodiment

Note: with use it is preceding compared with,^*P < 0.5,^**P < 0.01

Table 9 is compared using skin elasticity after the anti-ageing white different time of addition 5 anti-senile preparation of embodiment

Note: with use it is preceding compared with,^*P < 0.5,^**P < 0.01

By table 6-9 it is found that using addition embodiment 2-5 anti-apolexis composition it is anti-ageing frost after with use preceding comparison, use R2, R5, R7 value show the increase of the significance difference opposite sex after 20 days, show using the anti-ageing of addition embodiment 2-5 anti-apolexis composition Skin elasticity has clear improvement after frost.

Experimental example 1 and 2 confirms that 2-5 composition of the embodiment of the present invention has good inoxidizability and delays senescence and makees With.

Claims (8)

1. a kind of anti-aging cosmetics composition, it is characterised in that: the composition in parts by weight, forms as follows: caffeic acid benzene 15 parts of ethyl ester, 15 parts of propylene glycol, 10 parts of glycerol, 5 parts of 1,3-BDO, 1,2- 10 parts of pentanediol, 15 parts of the different monooctyl ester of ferulic acid, the Chinese 1.5 parts of raw rubber, 0.3g parts of Sodium Hyaluronate, 0.8 part of disodium ethylene diamine tetraacetate, 45 parts of emulsifier, 20 parts of cetostearyl alcohol, gather 20 parts of methylsiloxane, 45 parts of vegetable oil, 10 parts of freshener, VB₅8 parts, 6 parts of preservative, 3 parts of essence, surplus deionized water It is supplemented to 1000 parts by weight.

2. a kind of anti-aging cosmetics composition according to claim 1, it is characterised in that: the emulsifier is PEG-100 Stearate, paregal O mixture.

3. a kind of anti-aging cosmetics composition according to claim 1, it is characterised in that: the vegetable oil is dessert almond Oil, avocado oil, the mixture of shea butter.

4. a kind of anti-aging cosmetics composition according to claim 3, it is characterised in that: the vegetable oil is with parts by weight Meter, group become 3~8 parts of Sweet Almond Oil, 3~6 parts of avocado oil, 1~5 part of shea butter.

5. a kind of anti-aging cosmetics composition according to claim 1, it is characterised in that: the freshener is menthol.

6. a kind of anti-aging cosmetics composition according to claim 1, it is characterised in that: the preservative is benzene oxygen second Alcohol.

7. a kind of preparation method for preparing any anti-aging cosmetics composition of claim 1~6, which is characterized in that packet Include following steps:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase pot, 70~85 DEG C are heated to, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 70~85 DEG C, keep the temperature 15~30 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, is heated to 70~85 DEG C, to It is completely dissolved rear heat preservation for standby use；

D) by step a) in step c) oil mutually be pumped into step b) emulsion pot in, with stirring rate be 3000rpm stir 3~ It after five minutes, then with stirring rate is that 30rpm is stirred 15~45 minutes；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stir evenly.

8. a kind of preparation method of anti-aging cosmetics composition according to claim 7, it is characterised in that: including walking as follows It is rapid:

A) emulsifier, cetostearyl alcohol, vegetable oil, polymethyl siloxane, xanthan gum, the different monooctyl ester of ferulic acid are put into oily phase pot, 80 DEG C are heated to, heat preservation for standby use after waiting all components to dissolve；

B) above-mentioned glycerol, Sodium Hyaluronate, disodium ethylene diamine tetraacetate, deionized water are put into emulsion pot, is heated to 80 DEG C, protected Temperature 20 minutes；

C) by phenethyl caffeate, propylene glycol, 1,2- pentanediol, 1,3-BDO puts into oily phase pot, 78 DEG C is heated to, to complete Heat preservation for standby use after dissolution；

D) step a) is mutually pumped into the emulsion pot of step b) with the oil in step c), is that 3000rpm stirs 4 points with stirring rate Zhong Hou, then stirred 30 minutes with stirring rate for 30rpm；

E) lotion in step d) is cooled to 50~55 DEG C, above-mentioned VB is added₅, freshener, preservative, essence, stir evenly.