CN106107574A - A kind of preparation method of Sanguis sus domestica source compound microelement supplement - Google Patents
A kind of preparation method of Sanguis sus domestica source compound microelement supplement Download PDFInfo
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- CN106107574A CN106107574A CN201610658247.1A CN201610658247A CN106107574A CN 106107574 A CN106107574 A CN 106107574A CN 201610658247 A CN201610658247 A CN 201610658247A CN 106107574 A CN106107574 A CN 106107574A
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- sus domestica
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- 239000013589 supplement Substances 0.000 title claims abstract description 54
- 150000001875 compounds Chemical class 0.000 title claims abstract description 47
- 241000282894 Sus scrofa domesticus Species 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000000843 powder Substances 0.000 claims abstract description 41
- 239000000047 product Substances 0.000 claims abstract description 40
- 210000002381 plasma Anatomy 0.000 claims abstract description 36
- 210000000601 blood cell Anatomy 0.000 claims abstract description 23
- 239000011575 calcium Substances 0.000 claims abstract description 23
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 23
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000006228 supernatant Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000013522 chelant Substances 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 238000001556 precipitation Methods 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 4
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 4
- 239000001509 sodium citrate Substances 0.000 claims abstract description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 46
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 35
- 239000011701 zinc Substances 0.000 claims description 19
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 15
- 229910052725 zinc Inorganic materials 0.000 claims description 15
- 230000004044 response Effects 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 229960002089 ferrous chloride Drugs 0.000 claims description 6
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical group Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 6
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 238000005903 acid hydrolysis reaction Methods 0.000 claims description 4
- 108091005658 Basic proteases Proteins 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- 235000019419 proteases Nutrition 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- 108091005508 Acid proteases Proteins 0.000 claims description 2
- 108090000145 Bacillolysin Proteins 0.000 claims description 2
- 108010004032 Bromelains Proteins 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 102000035092 Neutral proteases Human genes 0.000 claims description 2
- 108091005507 Neutral proteases Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 239000000796 flavoring agent Substances 0.000 claims description 2
- 235000019634 flavors Nutrition 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 239000004606 Fillers/Extenders Substances 0.000 claims 1
- 239000001506 calcium phosphate Substances 0.000 claims 1
- 229910000389 calcium phosphate Inorganic materials 0.000 claims 1
- 235000011010 calcium phosphates Nutrition 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 235000019834 papain Nutrition 0.000 claims 1
- 229940055729 papain Drugs 0.000 claims 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 15
- 238000006243 chemical reaction Methods 0.000 abstract description 11
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 8
- 229920001184 polypeptide Polymers 0.000 abstract description 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 101000993059 Homo sapiens Hereditary hemochromatosis protein Proteins 0.000 abstract 1
- 229960005069 calcium Drugs 0.000 description 17
- 229910052742 iron Inorganic materials 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 239000011573 trace mineral Substances 0.000 description 5
- 235000013619 trace mineral Nutrition 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011669 selenium Substances 0.000 description 3
- 229910052711 selenium Inorganic materials 0.000 description 3
- 229940091258 selenium supplement Drugs 0.000 description 3
- 206010006956 Calcium deficiency Diseases 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
- 206010048259 Zinc deficiency Diseases 0.000 description 2
- IHBCFWWEZXPPLG-UHFFFAOYSA-N [Ca].[Zn] Chemical compound [Ca].[Zn] IHBCFWWEZXPPLG-UHFFFAOYSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000015424 sodium Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- DKKCQDROTDCQOR-UHFFFAOYSA-L Ferrous lactate Chemical compound [Fe+2].CC(O)C([O-])=O.CC(O)C([O-])=O DKKCQDROTDCQOR-UHFFFAOYSA-L 0.000 description 1
- 206010070840 Gastrointestinal tract irritation Diseases 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 206010022971 Iron Deficiencies Diseases 0.000 description 1
- 208000015710 Iron-Deficiency Anemia Diseases 0.000 description 1
- XPHUNVSXINXQTD-UHFFFAOYSA-N N1=CC=CC2=CC=CC=C12.O1C=CC=C1 Chemical compound N1=CC=CC2=CC=CC=C12.O1C=CC=C1 XPHUNVSXINXQTD-UHFFFAOYSA-N 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 206010044278 Trace element deficiency Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000036765 blood level Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- -1 calcium ferrum zinc compound Chemical class 0.000 description 1
- 239000004227 calcium gluconate Substances 0.000 description 1
- 235000013927 calcium gluconate Nutrition 0.000 description 1
- 229960004494 calcium gluconate Drugs 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 description 1
- UUVBYOGFRMMMQL-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca].OP(O)(O)=O UUVBYOGFRMMMQL-UHFFFAOYSA-N 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000004225 ferrous lactate Substances 0.000 description 1
- 235000013925 ferrous lactate Nutrition 0.000 description 1
- 229940037907 ferrous lactate Drugs 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 125000001841 imino group Chemical group [H]N=* 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000009984 peri-natal effect Effects 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229940091251 zinc supplement Drugs 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The present invention discloses the preparation method of a kind of Sanguis sus domestica source compound microelement supplement, comprises the following steps: Sanguis sus domestica is added sodium citrate anticoagulant by (1), centrifugal, generates blood plasma liquid and blood cell liquid, is separately dried prepared plasma powder and blood cell powder；(2) gained plasma powder and blood cell powder are carried out enzymolysis, generate blood plasma enzymolysis solution and blood cell enzymolysis solution, water-bath enzyme denaturing；(3) gained blood cell enzymolysis solution is centrifuged, and obtains supernatant and precipitation；(4) precipitation acidolysis is become ionic state；(5) carry out chelatropic reaction after gained blood plasma enzymolysis solution, supernatant and acidolysis solution being mixed, be dried to powder, obtain compound microelement supplement finished product.Preparation technology of the present invention, based on the utilization rate of Sanguis sus domestica raw material is greatly improved, can prepare the biotype compound microelement supplement of the polypeptide chelate metallic forms of high Fe contained and preferable calcium Zn content and additional product thereof, comprehensive process low cost, and added value of product is high.
Description
Technical field
The present invention relates to nutrient and healthcare products field, particularly relate to a kind of Sanguis sus domestica source calcium ferrum zinc compound microelement supplement
Preparation method.
Background technology
Human body is in order to maintain growth promoter and ensure health, and every day must be obtained including trace element by diet
Various nutrients, can affect normal physiological function, cause being chronically at sub-health state when intake is too low, and severe patient can be led
Cause multiple physiological disorder.Calcium, ferrum, zinc deficiency are a series of phases that a class causes owing to three kinds of elements of calcium deficiency ferrum zinc lack
Related disorders, it has also become Community health's problem the most serious in a world wide, especially to old people, child or gestation woman
The special populations such as female.Iron deficiency mainly results in iron deficiency anemia, causes phagocyte bactericidal reduced capability and immunity of organisms fall
Low；Calcium deficiency mainly shows as child's rickets, osteoporosis, osteomalacia, tic etc.；Zinc deficiency mainly shows as growth
Hypoevolutism, function of immune system are suppressed, the damage of perinatal stage infant brain, anorexia, Development of Reproductive System are bad etc..Along with people
Improve constantly and the continuous of health care consciousness of living standard strengthen, and while gradual perfection dietary structure, also begin to by battalion
Support supplement and carry out extra absorption to lacking trace element and supplement.
Occur in that miscellaneous functional for the exploitation of the trace element deficiency such as human calcium, ferrum, zinc in the market
Supplement, predominantly inorganic metal salt and organic acid-slaine, a kind of ferro-calcium-zinc-selenium as open in Chinese patent CN102228178
Granule, its component includes calcium lactate, calcium gluconate, ferrous lactate, sodium selenite etc..But inorganic metal salt and organic acid-gold
Belong to salt bioavailability in vivo relatively low, less stable, and there is side effect in various degree.Amino-acid metal chelate
The advantages such as GI irritation is little, stability is strong though class supplement have, easy absorption, but have that product is single, specificity is too strong and
The shortcoming that price is high.Polypeptide metal element chelate thing is by the N-Amino End Group of polypeptide, C-end carboxyl, amino acid side chain and peptide
Carbonyl and imino group in chain are formed with metallic ion coordination, the most inorganic/organic-metallic salt and amino acids metal element chelating
Thing has higher fit rate and stability, and it has the advantages such as biological value is high, absorb soon, trophism is strong, Yi Jikang simultaneously
Oxidation, antibacterial, immunomodulating, blood fat reducing and blood sugar lowering isoreactivity, have the highest Development volue and application prospect, be increasingly becoming
The focus of research both at home and abroad.
Sanguis sus domestica is a kind of side-product after pig is slaughtered, containing rich in protein, vitamin and mineral.It contains 18 kinds
Aminoacid, including 8 kinds of essential amino acids, additionally Sanguis sus domestica iron content is the abundantest, and according to surveying and determination, every 100g whole blood levels of iron content is up to
45mg, in porcine blood plasma, iron content is up to 6.4%, additionally, many possibly together with calcium, phosphorus, potassium, sodium, magnesium, zinc, copper, manganese, selenium etc. in Sanguis sus domestica
Plant trace element.At present, China more developed country in terms of Sanguis sus domestica exploitation falls behind, except part makees edible and processing blood powder
Outward, major part is not fully developed utilization.Therefore, comprehensive development and utilization Sanguis sus domestica resource, before having the most wide market
Scape.Chinese patent CN1112006 discloses a kind of tonic instant medicine granules for treatment of anaemia and preparation method thereof, and with fresh porcine blood as raw material, separation removes
Going liquid phase, solid content to add centrifugation after ethanol stirring, liquid phase reclaims ethanol, and solid content is dried, and after wearing into grade fine powder, needs additionally
Be aided with the components such as edible calcium, edible zinc and the Radix Astragali, Radix Polygoni Multiflori, Fructus Lycii, Fructus Jujubae, with this reach iron supplement, replenish the calcium, the merit of zinc supplement
Effect, Chinese patent CN101480254 discloses a kind of method utilizing enzymatic isolation method to extract peptide iron powder from Sanguis sus domestica, prepares polypeptide chelate
Ferrum iron supplement product；Chinese patent CN10337964 discloses a kind of method utilizing enzymatic isolation method to extract Sanguis sus domestica furan quinoline ferrum from Sanguis sus domestica.
But it is higher that above-mentioned existing supplement preparation technology exists integrated cost, wastage of material is serious, the shortcomings such as utilization rate is low, and trace unit
Element (ferrum calcium zinc etc.) yield and product nutritive validity need to improve further.
Summary of the invention
The drawbacks described above existed for existing compound microelement supplement and preparation technology, the present invention provides a kind of Sanguis sus domestica
The preparation method of source compound microelement supplement, the method makes full use of Sanguis sus domestica raw material, prepares ferrum calcium Zn content preferable
Supplement finished product, added value of product is greatly improved, reduces comprehensive process cost.
For realizing object above, technical scheme is as follows:
The preparation method of a kind of Sanguis sus domestica source compound microelement supplement, with fresh Sanguis sus domestica as raw material, comprises the following steps:
(1) Sanguis sus domestica is added sodium citrate anticoagulant, be centrifuged in 4000～6000r/min, generate blood plasma liquid and blood cell liquid, point
Gan Zao not prepare plasma powder and blood cell powder；
(2) step (1) gained plasma powder and blood cell powder are carried out enzymolysis, enzyme addition 0.01～2.5wt%, substrate respectively
Concentration 1.5～10wt%, enzymolysis pH is 3～11, hydrolysis temperature 30～60 DEG C, and enzymolysis time 1～10h generates plasma enzymes respectively
Solve liquid and blood cell enzymolysis solution, in 80～100 DEG C of water-bath enzyme denaturing 3～10min, standby；
(3) step (2) gained blood cell enzymolysis solution is centrifuged in 2500～5000r/min, obtains supernatant and precipitation, standby
With；
(4) the precipitation acidolysis of step (3) gained is become ionic state, standby；
(5) step (2) gained blood plasma enzymolysis solution, step (3) gained supernatant and step (4) gained acidolysis solution is mixed
Close, in 10～60 DEG C, chelate under the conditions of pH5～7, in the response time 0.5～10h, be dried to powder, obtain compound microelement
Supplement finished product 1.
Further, described method also comprises the steps: step (2) gained blood plasma enzymolysis solution, step (3) gained supernatant
Liquid and the mixing of step (4) gained acidolysis solution, add exogenous ferrum and/or calcium and/or zinc, in 10～60 DEG C, and pH5～7 conditions
Under chelate, in the response time 0.5～10h, be dried to powder, prepare compound microelement supplement addition product 1.
Further, described method also comprises the steps: step (2) gained blood plasma enzymolysis solution and step (3) gained
Supernatant mixes, and adds external source ferrum and/or calcium and/or zinc, chelates, during reaction under conditions of 10～60 DEG C, pH5～7
Between 0.5～10h, be dried to powder, prepare compound microelement supplement addition product 2.
Further, described method also comprises the steps；Take step (2) gained blood plasma enzymolysis solution, with step (4) gained
Acid hydrolysis solution mixes, and chelates, in the response time 0.5～10h, be dried to powder under conditions of 10～60 DEG C, pH5～7, prepares multiple
Close trace element replenisher addition product 3.
Further, described method also comprises the steps: to take step (2) gained blood plasma enzymolysis solution, according to 2～6: 1
Mass ratio adds exogenous ferrum and/or calcium and/or zinc, chelates, the response time under conditions of 10～60 DEG C, pH5～7
0.5～10h, it is dried to powder, prepares compound microelement supplement addition product 4.
Further, described method also comprises the steps: to take step (3) gained supernatant, according to the quality of 2～6: 1
Than adding exogenous ferrum and/or calcium and/or zinc, chelate under conditions of 10～60 DEG C, pH5～7, the response time 0.5～
10h, is dried to powder, prepares compound microelement supplement addition product 5.
Further, described method also comprises the steps: to take step (3) gained supernatant, with step (4) gained acidolysis
Liquid mixes, and chelates, in the response time 0.5～10h, be dried to powder under conditions of 10～60 DEG C, pH5～7, prepares compound micro-
Secondary element supplement addition product 6.
Specifically, step (2) described enzymolysis uses alkaline protease, acid protease, neutral protease, Papain
Enzyme, bromelain, flavor protease and animal protease one or more of which are compounding.
Specifically, described exogenous ferrum is selected from ferrous chloride or ferrous sulfate；Described exogenous calcium is selected from calcium carbonate, phosphoric acid
Calcium or calcium chloride, described exogenous zinc is selected from zinc chloride or zinc sulfate.
Specifically, described drying mode is selected from lyophilization, is vacuum dried or is spray-dried.
Specifically, the dilution heat of sulfuric acid of dilute hydrochloric acid solution, 1～the 9mol/L of step (4) described acidolysis employing 1～5mol/L
Or 36～the acetum of 38%.
Compared with existing compound microelement supplement and preparation technology, the present invention has the advantage that
The present invention, with Sanguis sus domestica as raw material, makes full use of the enzymolysis intermediate in preparation technology and place processes product blood
Slurry enzymolysis solution, blood cell enzymolysis solution, blood cell enzymolysis solution supernatant, blood cell enzymolysis solution precipitation and acid hydrolysis solution etc., coordinate employing specific simultaneously
Enzymolysis, separating, process conditions and the parameter such as be dried, disposably prepared, gained compound microelement supplement comprise with excellent
Matter polypeptide metal element chelate thing form exist high content iron and the preferable calcium of content, zinc element (comprise simultaneously phosphorus, potassium,
The various trace elements such as sodium, magnesium, copper, manganese, selenium), it is not necessary to the exogenous ferrum calcium zinc element of extra interpolation i.e. has stronger nutrition again
Supplementary functions, is the ideal biological type compound microelement supplement that a absorption is fast, effective, indication scope is wide.Additionally,
Than traditional handicraft, these supplement preparation technology, based on Sanguis sus domestica raw material availability is greatly improved, significantly reduces comprehensive process
This, improve added value of product, energy-conserving and environment-protective, have good economic benefit and wide market application foreground.
Accompanying drawing explanation
Fig. 1 is the preparation technology flow chart of Sanguis sus domestica source of the present invention compound microelement supplement.
Detailed description of the invention
Below in conjunction with the accompanying drawings, it is described further by the detailed description of the invention of the embodiment present invention.
Preparation embodiment
Embodiment 1
(1) fresh Sanguis sus domestica 1kg is added the sodium citrate anticoagulant of 2.5wt%, be centrifuged (4000r/min ,-4 DEG C, 20min),
Generating blood cell liquid and blood plasma liquid, lyophilizing obtains 150g plasma powder and 40g blood cell powder；
(2) blood cell powder and plasma powder are carried out respectively enzymolysis (alkaline protease, original ph 10, concentration of substrate 8wt%,
Enzyme addition 0.4wt%, enzymolysis time 4h, hydrolysis temperature 50 DEG C), generate blood plasma enzymolysis solution and blood cell enzymolysis solution, 100 DEG C of water-baths
Enzyme denaturing 5min, standby；
(3) blood cell enzymolysis solution centrifugal (4000r/min ,-4 DEG C, 10min), obtains supernatant and precipitation, standby；
(4) 3mol/L dilute hydrochloric acid solution (dilute hydrochloric acid solution and precipitation volume are used in step (3) gained is deposited in centrifuge tube
Ratio 1: 1) acidolysis, generate ionic state ferrum；
(5) step (2) gained blood plasma enzymolysis solution, step (3) gained supernatant and step (4) gained acidolysis solution is mixed
Close, regulate pH to 6, carry out chelatropic reaction 3h in 25 DEG C, be lyophilized into powder, obtain compound microelement supplement finished product 1.
Embodiment 2
On the basis of step described in embodiment 1, take step (2) gained blood plasma enzymolysis solution 1000ml, step (3) gained supernatant
Liquid 350ml mixes with step (4) gained acidolysis solution 100ml, and the mass ratio according to 4: 1 adds ferrous chloride, in pH 7.0,25
Carry out chelatropic reaction 3h under the conditions of DEG C, be lyophilized into powder, prepare compound microelement supplement addition product 1.
Embodiment 3
On the basis of step described in embodiment 1, take on step (2) gained blood plasma enzymolysis solution 1000ml and step (3) gained
Clear liquid 350ml, the mass ratio according to 4: 1 adds ferrous chloride, in pH6.0, carries out chelatropic reaction 3h, be lyophilized under the conditions of 25 DEG C
Powder, prepares compound microelement supplement addition product 2.
Embodiment 4
On the basis of step described in embodiment 1, take step (2) gained blood plasma enzymolysis solution 1000ml and the acid of step (4) gained
Solve liquid 100ml, in pH 6.0, carry out chelatropic reaction 3h under the conditions of 25 DEG C, be vacuum dried into powder, prepare compound microelement and supplement
Agent addition product 3.
Embodiment 5
On the basis of step described in embodiment 1, take step (2) gained blood plasma enzymolysis solution 1000ml, according to the mass ratio of 4: 1
Add ferrous chloride, in pH 7.0, carry out chelatropic reaction 3h under the conditions of 25 DEG C, be vacuum dried into powder, prepare compound microelement
Supplement addition product 4.
Embodiment 6
On the basis of step described in embodiment 1, taking step (3) gained supernatant 350ml, the mass ratio according to 4: 1 adds
Ferrous chloride, in pH7.0, carries out chelatropic reaction 3h, is lyophilized into powder under the conditions of 25 DEG C, prepare compound microelement supplement and add
Product 5.
Embodiment 7
On the basis of step described in embodiment 1, take step (3) gained supernatant 350ml and step (4) gained acidolysis solution
100ml mixes, and in pH7.0, carries out chelatropic reaction 3h, be lyophilized into powder under the conditions of 25 DEG C, prepares compound microelement supplement attached
Add product 6.
Embodiment 8
On the basis of step described in embodiment 1, take step (2) gained blood plasma enzymolysis solution 1000ml, step (3) gained supernatant
Liquid 350ml mixes with step (4) gained acidolysis solution 100ml, and the mass ratio according to 4: 1 adds calcium carbonate, in pH 5.0,25 DEG C
Under the conditions of carry out chelatropic reaction 3h, be lyophilized into powder, prepare compound microelement supplement addition product 7.
Embodiment 9
On the basis of step described in embodiment 1, take step (2) gained blood plasma enzymolysis solution 1000ml, step (3) gained supernatant
Liquid 350ml mixes with step (4) gained acidolysis solution 100ml, and the mass ratio according to 4: 1 adds zinc chloride, in pH 7.0,25 DEG C
Under the conditions of carry out chelatropic reaction 3h, be lyophilized into powder, prepare compound microelement supplement addition product 8.
Using plasma aes determination calcium ferrum Zn content, concrete measurement result is as shown in the table:
Table 1 calcium ferrum zinc-content determination result
As seen from the above table, the present invention made compound microelement supplement finished product and additional product have higher ferrum and contain
Amount and preferably calcium, Zn content.
From above-described embodiment, preparation technology of the present invention drastically increases the utilization rate of Sanguis sus domestica raw material, can prepare height
The biotype compound microelement supplement of the polypeptide chelate metallic forms of iron-holder and preferable calcium Zn content and additional product thereof, combine
Conjunction processing cost is low, and added value of product is high.
If no special instructions, involved by embodiment 1～embodiment 9, experimental facilities, instrument and raw material are commercially available general product
Product.
It is understood that above with respect to the specific descriptions of the present invention, be merely to illustrate the present invention and be not limited to this
Technical scheme described by inventive embodiments.It will be understood by those within the art that, still the present invention can be carried out
Amendment or equivalent, to reach identical technique effect；As long as meet use needs, all protection scope of the present invention it
In.
Claims (10)
1. a preparation method for Sanguis sus domestica source compound microelement supplement, with fresh Sanguis sus domestica as raw material, it is characterised in that include
Following steps:
(1) Sanguis sus domestica is added sodium citrate anticoagulant, be centrifuged in 4000～6000r/min, generate blood plasma liquid and blood cell liquid, do respectively
Dry prepared plasma powder and blood cell powder；
(2) step (1) gained plasma powder and blood cell powder are carried out enzymolysis, enzyme addition 0.01～2.5wt%, concentration of substrate respectively
1.5～10wt%, enzymolysis pH are 3～11, hydrolysis temperature 30～60 DEG C, enzymolysis time 1～10h, generate blood plasma enzymolysis solution respectively
With blood cell enzymolysis solution, in 80～100 DEG C of water-bath enzyme denaturing 3～10min, standby；
(3) step (2) gained blood cell enzymolysis solution is centrifuged in 2500～5000r/min, obtains supernatant and precipitation, standby；
(4) the precipitation acidolysis of step (3) gained is become ionic state, standby；
(5) step (2) gained blood plasma enzymolysis solution, step (3) gained supernatant and step (4) gained acidolysis solution are mixed, in
10～60 DEG C, chelate under the conditions of pH5～7, in the response time 0.5～10h, be dried to powder, obtain compound microelement and supplement
Agent finished product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: step (2) gained blood plasma enzymolysis solution, step (3) gained supernatant and the mixing of step (4) gained acidolysis solution, add
Enter exogenous ferrum and/or calcium and/or zinc, in 10～60 DEG C, chelate under the conditions of pH5～7, the response time 0.5～10h, dry
Dry one-tenth powder, prepares compound microelement supplement addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: step (2) gained blood plasma enzymolysis solution is mixed with step (3) gained supernatant, add external source ferrum and/or calcium and/
Or zinc, chelate under conditions of 10～60 DEG C, pH5～7, in the response time 0.5～10h, be dried to powder, prepare compound trace
Component extender addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: take step (2) gained blood plasma enzymolysis solution and mix with step (4) gained acid hydrolysis solution, in the bar of 10～60 DEG C, pH5～7
Chelate under part, in the response time 0.5～10h, be dried to powder, prepare compound microelement supplement addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: take step (2) gained blood plasma enzymolysis solution, the mass ratio according to 2～6: 1 add exogenous ferrum and/or calcium and/or
Zinc, chelates under conditions of 10～60 DEG C, pH5～7, in the response time 0.5～10h, is dried to powder, prepares compound trace unit
Element supplement addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: take step (3) gained supernatant, the mass ratio according to 2～6: 1 adds exogenous ferrum and/or calcium and/or zinc, in
Chelate under conditions of 10～60 DEG C, pH5～7, in the response time 0.5～10h, be dried to powder, prepare compound microelement and mend
Fill agent addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that also include
Following steps: take step (3) gained supernatant and mix with step (4) gained acid hydrolysis solution, under conditions of 10～60 DEG C, pH5～7
Chelate, in the response time 0.5～10h, be dried to powder, prepare compound microelement supplement addition product.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that: step
(2) described enzymolysis uses alkaline protease, acid protease, neutral protease, papain, bromelain, local flavor egg
White enzyme and animal protease one or more of which compound.
9., according to the preparation method of the Sanguis sus domestica source compound microelement supplement described in claim 2,3,5 or 6, its feature exists
In: described exogenous ferrum is selected from ferrous chloride or ferrous sulfate, and described exogenous calcium is selected from calcium carbonate, calcium phosphate or calcium chloride,
Described exogenous zinc is selected from zinc chloride or zinc sulfate.
The preparation method of Sanguis sus domestica source the most according to claim 1 compound microelement supplement, it is characterised in that: step
(4) described acidolysis use 1～5mol/ the dilution heat of sulfuric acid of dilute hydrochloric acid solution, 1～9mol/L or 36～38% acetic acid molten
Liquid.
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| CN109090490A (en) * | 2018-08-31 | 2018-12-28 | 常同喜 | A kind of preparation method of calcium-supplementing preparation |
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Application publication date: 20161116 |