CN103960699B - A kind of krill peptide replenishers containing calcium iron zinc - Google Patents
A kind of krill peptide replenishers containing calcium iron zinc Download PDFInfo
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- CN103960699B CN103960699B CN201410222639.4A CN201410222639A CN103960699B CN 103960699 B CN103960699 B CN 103960699B CN 201410222639 A CN201410222639 A CN 201410222639A CN 103960699 B CN103960699 B CN 103960699B
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- 241000239366 Euphausiacea Species 0.000 title claims abstract description 103
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 46
- JYMOQWNEIZBVFC-UHFFFAOYSA-N [Fe].[Zn].[Ca] Chemical compound [Fe].[Zn].[Ca] JYMOQWNEIZBVFC-UHFFFAOYSA-N 0.000 title claims abstract description 16
- 239000011575 calcium Substances 0.000 claims abstract description 23
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000005238 degreasing Methods 0.000 claims abstract description 13
- KFZAUHNPPZCSCR-UHFFFAOYSA-N iron zinc Chemical compound [Fe].[Zn] KFZAUHNPPZCSCR-UHFFFAOYSA-N 0.000 claims abstract description 11
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims abstract description 10
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 claims abstract description 10
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- 239000000843 powder Substances 0.000 claims abstract description 9
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- 239000006228 supernatant Substances 0.000 claims description 61
- 239000000243 solution Substances 0.000 claims description 32
- 239000004365 Protease Substances 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 14
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
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- 238000009413 insulation Methods 0.000 claims description 6
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- 238000004108 freeze drying Methods 0.000 claims description 4
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- 238000000034 method Methods 0.000 claims description 3
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- XQKKWWCELHKGKB-UHFFFAOYSA-L calcium acetate monohydrate Chemical compound O.[Ca+2].CC([O-])=O.CC([O-])=O XQKKWWCELHKGKB-UHFFFAOYSA-L 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 15
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 18
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 11
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 1
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- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
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- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 206010048259 Zinc deficiency Diseases 0.000 description 1
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 1
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- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/40—Shell-fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The object of the present invention is to provide a kind of krill peptide replenishers containing calcium iron zinc, the described krill peptide replenishers containing calcium iron zinc are attached to calcium iron zinc metallic element by euphausia superba powder degreasing, proteasome degradation and CaCl
2on the krill Gly-His-Lys that defluorinate is made.The abundant raw material that the present invention adopts is easy to get, and protein content is high; Preparation method has that processing step is simple, equipment investment is few, non-environmental-pollution, product yield advantages of higher, is suitable for suitability for industrialized production; Prepared krill peptide-calcium iron Zinc supplements has following feature: protein content is higher than 90%, and product purity is high; Oil repellent is lower than 10ppm, and Product Safety is high; Molecular weight is mainly distributed in 2000-200Da, good water solubility; Essential amino acids content is high, and calcium iron zinc composition rationally, can supplement three kinds of nutrients and essential amino acid simultaneously.
Description
Technical field
The invention belongs to technical field of functional food, be specifically related to a kind of krill peptide replenishers containing calcium iron zinc, and preparation method thereof and application.
Background technology
Calcium, iron, zinc dificiency are a series of relevant diseases that a class causes because calcium iron zinc three kinds of mineral elements lack, become Community health's problem serious all the more in a world wide, especially for special populations such as the elderly, children and pregnancy women.The illness such as zinc deficiency main manifestations is growth retardation, function of immune system is suppressed, infant brain damage perinatal period, apocleisis, skin infection and fecundity go down.Asiderosis main manifestations is that cytophagous sterilizing ability weakens and the illnesss such as immunity of organisms reduction.Calcium deficiency main manifestations is the illnesss such as osteoporosis, hypertension, colon cancer, obesity and kidney stone.According to Chinese residents nutrition in 2004 and Health Situation investigation statistics, resident's prevalence of anemia average out to 15.2%, within 2 years old, baby, more than 60 years old old man and women of child-bearing age's prevalence of anemia are respectively 24.2%, 21.5% and 20.6%; Resident's calcium intake is only 41% of recommended intake; Less than 5 years old growthretardation rates and under-weight rate are respectively 17.3% and 9.3%.
Relevant hardening agent product is in the market mainly inorganic metal salt and organic acid-slaine.Wherein, the calcium in inorganic metal salt and organic acid-slaine, iron, zinc absorption are less than 40%.And medical expert's research shows, the absorption of calcium iron zinc three kinds of elements in body can be coordinated mutually, absorption of mutually promoting.The science combination of three kinds of elements can improve the shortage of body calcium iron zinc effectively, meets the demand of health to calcium iron zinc.Clinical research shows, when lacking three kinds of elements, supplementary wherein one can not reach and improve effect separately simultaneously.Calcium iron zinc compound formulation then can reach increase children appetite, improves rickets, increases Hemoglobin Value and promote children's intelligence and healthy effect of growing.Therefore, finding the safe and effective nutrition fortifier that simultaneously can supplement calcium iron zinc is current study hotspot.
Summary of the invention
The object of the present invention is to provide a kind of krill peptide replenishers containing calcium iron zinc, and preparation method thereof and application, thus make up the deficiencies in the prior art.
Krill peptide replenishers containing calcium iron zinc of the present invention prepare by following method:
1) by euphausia superba powder after Ethanol Treatment, drying and crushing obtains degreasing krill meal;
2) in degreasing krill meal, add distilled water, feed liquid mass ratio is 1:8 ~ 12, regulates solution ph to be 7.0 ~ 8.0, then in solution, adds protease be hydrolyzed, and hydrolysis terminates rear heating termination enzyme digestion reaction, centrifuging and taking supernatant A;
3) pH value of supernatant A is adjusted to 8.5 ~ 9.5, in supernatant A, adds CaCl
2defluorinate, leave standstill after mixing, centrifuging and taking supernatant B, by supernatant B adjust ph to 7.0 ~ 7.5, supernatant B crosses NF membrane desalination, spray-dried krill Gly-His-Lys;
4) in krill Gly-His-Lys, add distilled water and be made into krill peptide solution, solution ph is adjusted to 4 ~ 6, in krill peptide solution, add ferrous salt and ascorbic acid, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant C and obtains krill peptide-ferrous compound water solution;
5) pH value of supernatant C is adjusted to 6 ~ 8, in supernatant C, adds zinc salt, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant D and obtains krill peptide-ferrous iron-zinc complexes aqueous solution;
6) in supernatant D, add calcium salt, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant E and obtains krill peptide-ferrous iron-zinc-calcium complexes aqueous solution;
7) supernatant E is crossed NF membrane desalination, after freeze drying, obtain krill peptide-calcium iron Zinc supplements.
Wherein protease described in step (2) is one or more in food-grade papain, bromelain, trypsase, neutral proteinase or alkali protease, wherein with alkali protease best results, hydrolysis temperature is 50 DEG C, solution ph is 8.0, and hydrolysis time is 2-6h;
CaCl described in step (3)
2addition be 1-2% (w/v), NF membrane molecular cut off is 100Da;
Described in step (3), spray drying condition is: in solution, solid content is 8-15%, and EAT is 130-140 DEG C, and leaving air temp is 80-100 DEG C;
Described in step (4), the concentration of krill peptide solution is 1-20%, ferrous salt is the one in frerrous chloride, ferrous sulfate or iron ammonium sulfate, ferrous salt concentration is 0.05-0.2mmol/ every g krill peptide, and ascorbic acid concentrations is 0.05-0.5% (w/v);
Zinc salt described in step (5) is the one in zinc chloride, zinc sulfate or zinc acetate, and zinc salt concentration is 0.1-0.5mmol/g-krill peptide;
Calcium salt described in step (6) is the one in calcium chloride or calcium acetate, and calcium concentration is 3-10mmol/g-krill peptide;
Described in step (7), NF membrane molecular cut off is 100Da.
It is raw material that the present invention establishes first with krill, the technological process of preparation krill peptide-calcium iron Zinc supplements, and the abundant raw material of employing is easy to get, and protein content is high; Preparation method has that processing step is simple, equipment investment is few, non-environmental-pollution, product yield advantages of higher, is suitable for suitability for industrialized production; Prepared krill peptide-calcium iron Zinc supplements has following feature: protein content is higher than 90%, and product purity is high; Oil repellent is lower than 10ppm, and Product Safety is high; Molecular weight is mainly distributed in 2000-200Da, good water solubility, is easy to absorption of human body; Essential amino acids content is high, and calcium iron Zn content is reasonable, and both having supplemented calcium, iron, zinc three kinds of nutrients simultaneously, and supplemented essential amino acid again, is a kind of excellent replenishers.
Accompanying drawing explanation
Fig. 1: the graph of molecular weight distribution of krill peptide prepared by the present invention, its middle-molecular-weihydroxyethyl is the chromatogram that the curve of 6512Da, 1355Da and 429Da is respectively corresponding molecular weight standards.
Fig. 2: the infrared spectrogram of krill peptide of the present invention and metal composite thereof; Wherein, (a) is the infrared spectrogram of krill peptide; B () is the infrared spectrogram of krill peptide-iron complexes; C () is the infrared spectrogram of krill peptide-iron-zinc complexes; D () is the infrared spectrogram of krill peptide-iron-zinc-calcium complexes.
Detailed description of the invention
Krill is a class oceanic invertebrate, is distributed widely in each ocean, the world.According to statistics, the reserves of krill are 6.5-10 hundred million tons, are the huge protein resource treasure-houses of the mankind.In krill dry weight, protein content is about 65-75%, and its amino acid composition is reasonable, essential amino acids content is high, is the important source material of carrying out protein higher value application.At present, the application and development of krill mainly concentrates on shrimp sauce, astaxanthin and chitin aspect, and its protein is mainly used in fishery bait.Adopting protease hydrolytic krill, preparation low-molecular-weight krill peptide, with krill peptide for substrate prepares krill peptide-iron-zinc-calcium compound replenishers, is to one of effective way of krill albumen higher value application.
Below in conjunction with specific embodiment, krill peptide replenishers containing calcium iron zinc of the present invention and preparation method thereof are described in detail.
Embodiment 1
1) get euphausia superba powder 10kg, add 95% ethanol 100L, stir 6h in a kettle. in krill meal, centrifuging and taking precipitates, and vacuum drying obtains degreasing krill meal;
2) extracting degreasing shrimp med 5kg, adds distilled water 60L, is warming up to 50 DEG C in degreasing shrimp med, in solution, add 2MHCl regulate solution ph to 7.5, in solution, add neutral proteinase 75g, constant temperature hydrolysis 6h, be heated to 100 DEG C and stop enzyme reaction, centrifuging and taking supernatant A;
3) regulate the pH value to 9.0 of supernatant A, in supernatant A, add anhydrous CaCl
2900g, after mixing, 20 DEG C of standing 30min, centrifuging and taking supernatant B, the pH value to 7.2 that 2MHCl regulates supernatant B is added in supernatant B, supernatant B crosses NF membrane desalination, and spray-dried krill Gly-His-Lys, the molecular weight distribution of gained krill Gly-His-Lys is mainly 2000-200Da (see accompanying drawing 1).In krill Gly-His-Lys, essential amino acids content comparatively krill meal and degreasing krill meal is all significantly increased (table 1), its essential amino acids content meets the regulation of FAO/WHO (1985) for essential amino acids content in adult's desired protein, substantially meets the regulation (wherein valine, histidine, leucine and sulfur amino acid content a little less than regulation) of FAO/WHO (1985) for essential amino acids content in baby's desired protein.
Table 1: the amino acid composition analysis result of krill peptide prepared by the present invention, euphausia superba powder and degreasing euphausia superba powder.
4) get krill peptide powder 2kg, in krill Gly-His-Lys, add distilled water 20L, be made into krill peptide solution, in solution, add 2MHCl, regulate solution ph to 5.0, add 68.63gFeCl
24H
2o and 20g ascorbic acid, is incubated 2h under 25 DEG C of conditions, centrifuging and taking supernatant C, and supernatant C is krill peptide-ferrous compound water solution.After krill peptide and ferrous ion compound, the peak position of its infrared spectrum bands of a spectrum there occurs significant change (see accompanying drawing 2a and 2b), wherein, is positioned at 1455cm
-1the vibration peak at place disappears; Be positioned at 1587cm
-1and 1663cm
-1the vibration peak peak position at place moves respectively to lower wave number place, i.e. 1578cm
-1and 1659cm
-1, illustrate that ferrous ion and krill peptide interact, generate new compound, be i.e. krill peptide-ferrous compound.
5) in supernatant C, add 2MNaOH, regulate the pH value to 7.5 of supernatant C, in supernatant C, add 54.52gZnCl
2, under 25 DEG C of conditions, be incubated 2h, centrifuging and taking supernatant D, supernatant D are krill peptide-ferrous iron-zinc complexes aqueous solution.After krill peptide-ferrous compound and zinc ion compound, the peak position of Partial IR bands of a spectrum continues to move (see accompanying drawing 2b and 2c) to lower wave number direction, wherein, is positioned at 1406cm
-1, 1630cm
-1and 1659cm
-1the vibration peak peak position at place moves to 1405cm respectively
-1, 1629cm
-1and 1655cm
-1place; Be positioned at 1578cm
-1the vibration peak peak position at place moves to 1590cm to high wave number direction
-1, illustrate that in krill peptide molecule, side chain vibration is strengthened, the hydrogen bond action that intramolecule is relevant to side chain weakens; 1340cm
-1there is significant infrared vibration peak in place, illustrates that zinc ion and krill peptide-ferrous compound interact, generate new compound, be i.e. krill peptide-ferrous iron-zinc complexes.
6) in supernatant D, 2.60kgCaCl is added
2, under 25 conditions, be incubated 2h, centrifuging and taking supernatant E, supernatant E are krill peptide-ferrous iron-zinc-calcium complexes aqueous solution.After krill peptide-ferrous iron-zinc complexes and calcium ion compound, the peak position of Partial IR bands of a spectrum moves (see accompanying drawing 2c and 2d) to high wave number direction, wherein, is positioned at 1655cm
-1, 1405cm
-1and 1340cm
-1the vibration peak peak position at place moves to 1661cm respectively
-1, 1414cm
-1and 1343cm
-1place; Be positioned at 1590cm
-1the vibration peak peak position at place moves to 1570cm to lower wave number direction
-1place, illustrates that calcium ion and krill peptide-ferrous iron-zinc complexes interact, generates new compound, be i.e. krill peptide-ferrous iron-zinc-calcium complexes.
7) supernatant E crosses NF membrane desalination, after freeze drying, obtains krill peptide-calcium iron Zinc supplements.
The present invention is prepared in krill peptide-calcium iron Zinc supplements, and iron content is 2.40mg-Fe/g-product, and Zn content is 8.28mg-Zn/g-product, and calcium content is 145.38mg-Ca/g-product.According to Chinese residents dietary guidelines to the regulation of nutrient recommended intake every day: infant, every day, calcium recommended intake was 600-800mg/g, and every day, zinc recommended intake was 9-12mg/g, and every day, iron recommended intake was 10-12mg/g; Adult's calcium recommended intake every day is 800mg/g, and every day, zinc recommended intake was 11.5-15mg/g, and every day, iron recommended intake was 15-20mg/g.Replenishers prepared by the present invention can reasonable supplement three kinds of nutrients, improve health.
Embodiment 2
(1) get euphausia superba powder 10kg, add 95% ethanol 80L, stir 7h in a kettle. in krill meal, centrifuging and taking precipitates, and vacuum drying obtains degreasing krill meal;
(2) extracting degreasing shrimp med 5kg, adds distilled water 50L, in solution, adds 2MHCl in degreasing shrimp med, regulate solution ph to 8.0, be warming up to 50 DEG C, add alkali protease 100g, constant temperature hydrolysis 4h, is heated to 100 DEG C and stops enzyme reaction, centrifuging and taking supernatant A;
(3) regulate the pH value to 8.5 of supernatant A, in supernatant A, add anhydrous CaCl
2600g, after mixing, 20 DEG C of standing 30min, centrifuging and taking supernatant B, adds 2MHCl in supernatant B, regulates the pH value of supernatant B to 7.0-7.5, supernatant B crosses NF membrane desalination, and spray-dried krill peptide powder, the molecular weight distribution of gained krill Gly-His-Lys is mainly 2000-200Da.
(4) get krill peptide 2kg, in krill peptide, add distilled water 30L, be made into krill peptide solution, in solution, add 2MHCl, regulate solution ph to 4.5, add 79.50gFeCl
24H
2o and 25g ascorbic acid, is incubated 2h under 37 DEG C of conditions, centrifuging and taking supernatant C, and supernatant C is krill peptide-ferrous compound water solution;
(5) in supernatant C, add 2MNaOH, regulate the pH value to 7.0 of supernatant C, in solution, add 40.89gZnCl
2, under 37 DEG C of conditions, be incubated 2h, centrifuging and taking supernatant D, supernatant D are krill peptide-ferrous iron-zinc complexes aqueous solution;
(6) in supernatant D, 2kgCaCl is added
2, under 37 DEG C of conditions, be incubated 2h, centrifuging and taking supernatant E, supernatant E are krill peptide-ferrous iron-zinc-calcium complexes aqueous solution;
(7) supernatant E crosses NF membrane desalination, after freeze drying, obtains krill peptide-calcium iron Zinc supplements.
After testing, in krill peptide-calcium iron Zinc supplements, iron content is 2.78mg-Fe/g-product, and Zn content is 8.35mg-Zn/g-product, and calcium content is 137.64mg-Ca/g-product.
Krill peptide prepared by the present invention-calcium iron Zinc supplements has following feature: protein content is higher than 90%, and product purity is high; Oil repellent is lower than 10ppm, and Product Safety is high; Molecular weight is mainly distributed in 2000-200Da, good water solubility, is easy to absorption of human body; Essential amino acids content is high, and calcium iron Zn content is reasonable, and both having supplemented calcium, iron, zinc three kinds of nutrients simultaneously, and supplemented essential amino acid again, is a kind of excellent replenishers.
Claims (7)
1. the krill peptide replenishers containing calcium iron zinc, it is characterized in that, described replenishers prepare by following method:
1) by euphausia superba powder after Ethanol Treatment, drying and crushing obtains degreasing krill meal;
2) in degreasing krill meal, add distilled water, feed liquid mass ratio is 1:8 ~ 12, and regulate solution ph to be 7.0 ~ 8.0, then in solution, add protease be hydrolyzed, hydrolysis temperature is 50 DEG C, and solution ph is 8.0, and hydrolysis time is 2-6h; Hydrolysis terminates rear heating termination enzyme digestion reaction, centrifuging and taking supernatant A;
3) pH value of supernatant A is adjusted to 8.5 ~ 9.5, in supernatant A, adds CaCl
2defluorinate, leave standstill after mixing, centrifuging and taking supernatant B, by supernatant B adjust ph to 7.0 ~ 7.5, supernatant B crosses NF membrane desalination, spray-dried krill Gly-His-Lys;
4) in krill Gly-His-Lys, add distilled water and be made into krill peptide solution, solution ph is adjusted to 4 ~ 6, in krill peptide solution, add ferrous salt and ascorbic acid, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant C and obtains krill peptide-ferrous compound water solution;
Wherein ferrous salt is any one or several in frerrous chloride, ferrous sulfate or iron ammonium sulfate, and ferrous salt and the proportionate relationship of krill peptide are the corresponding 0.05-0.2mmol ferrous salt of every g krill peptide;
5) pH value of supernatant C is adjusted to 6 ~ 8, in supernatant C, adds zinc salt, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant D and obtains krill peptide-ferrous iron-zinc complexes aqueous solution; Wherein zinc salt is any one or several in zinc chloride, zinc sulfate or zinc acetate, and zinc salt and the proportionate relationship of krill peptide are the corresponding 0.1-0.5mmol zinc salt of every g krill peptide;
6) in supernatant D, add calcium salt, insulation reaction under 25-45 DEG C of condition, reaction terminates rear centrifuging and taking supernatant E and obtains krill peptide-ferrous iron-zinc-calcium complexes aqueous solution; Wherein calcium salt is any one or two kinds of in calcium chloride or calcium acetate, and calcium salt and the proportionate relationship of krill peptide are the corresponding 3-10mmol calcium salt of every g krill peptide;
7) supernatant E is crossed NF membrane desalination, after freeze drying, obtain krill peptide-calcium iron Zinc supplements.
2. krill peptide replenishers as claimed in claim 1, is characterized in that, described step 2) in protease be one or more in food-grade papain, bromelain, trypsase, neutral proteinase or alkali protease.
3. krill peptide replenishers as claimed in claim 2, is characterized in that, described step 2) in protease be alkali protease.
4. krill peptide replenishers as claimed in claim 1, is characterized in that, described step 3) middle CaCl
2addition be 1-2%.
5. krill peptide replenishers as claimed in claim 1, is characterized in that, described step 3) in spray drying condition as follows: in solution, solid content is 8-15%, and EAT is 130-140 DEG C, and leaving air temp is 80-100 DEG C.
6. krill peptide replenishers as claimed in claim 1, is characterized in that, described step 4) in the concentration of krill peptide solution be 1-20%.
7. krill peptide replenishers as claimed in claim 1, is characterized in that, described step 4) in ascorbic acid concentrations be 0.05-0.5%.
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| CN104388176B (en) * | 2014-11-10 | 2017-01-25 | 大连工业大学 | Method for preparing euphausia superba oil, microcapsule of euphausia superba oil and low-fluorine euphausia superba peptide by using aqueous enzymatic method |
| CN105265998A (en) * | 2015-09-15 | 2016-01-27 | 广东海洋大学 | Preparation method of antarctic krill low-fluoride enzymatic hydrolysate |
| AU2019214524A1 (en) * | 2018-01-30 | 2020-08-27 | Aker Biomarine Antarctic As | Marine protein hydrolysate with low fluoride and trimethylamin content |
| CN108588157A (en) * | 2018-04-24 | 2018-09-28 | 大连理工大学 | The method that combination enzymic degradation degreasing euphausia superba powder recycles polypeptide intermediate product and prepares N-Acetyl-D-glucosamine |
| CN108771151A (en) * | 2018-05-21 | 2018-11-09 | 岭南师范学院 | A kind of method that medium temperature prepares sterile shrimping beam trawl dry powder |
| CN109897877A (en) * | 2019-03-04 | 2019-06-18 | 大连工业大学 | The preparation method of krill peptide ferrous chelate compound |
| CN110037281A (en) * | 2019-04-08 | 2019-07-23 | 大连工业大学 | Iron strengthens krill sauce and preparation method thereof |
| CN109880871A (en) * | 2019-04-18 | 2019-06-14 | 威海市宇王集团海洋生物工程有限公司 | A method of small-molecular peptides are extracted by raw material of euphausia superba powder |
| CN110731512A (en) * | 2019-11-12 | 2020-01-31 | 大连工业大学 | Antarctic krill iron chelate peptide and preparation method and application of iron chelate peptide and iron chelate thereof |
| CN117568430A (en) * | 2023-11-07 | 2024-02-20 | 中国海洋大学 | Antarctic krill peptide with uric acid reducing activity and application thereof |
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