CN101664193A - Preparation method of rana chensinensis iron supplement capsules - Google Patents

Abstract

The invention relates to a preparation method of rana chensinensis iron supplement capsules, belonging to the agricultural product processing technique. The preparation method comprises the followingsteps: taking dried rana chensinensises as raw materials, drying, powdering, leaching and degreasing to obtain purified degreased dried rana chensinensises powder; under mild conditions, preparing compound hydrolyzed solution of the dried rana chensinensises amino acid and peptide by enzymolysis of various proteolytic enzymes; and then taking ferrous chloride as an iron source to prepare chelatedpeptide iron supplement capsules of rana chensinensis compound chelated peptide iron by the chelation. The method spreads the application range of the dried rana chensinensis and guarantees the continuous utilization of the rana chensinensis resource and has the characteristics of reasonable and simple process and high utilization ratio of the dried rana chensinensis, and the product facilitates the absorption of iron by human body.

Description

The production method of wood frog iron supplement capsules

Technical field

The invention belongs to technical field of agricultural product process, relate in particular to a kind of wood frog chelating peptide iron production method of health food-Yizhibao.

Background technology

Rana temporaria chensinensis (Rana temporaria chensinensis) main product is food, the dual-purpose precious frog kind of medicine in the Heilongjiang Province, and the dry product of its fallopian tube of female frog is used as medicine, and name " OVIDUCTUS RANNAE ", " oviductus ranae " are the animal drugs of tradition, produce effects.Clearly record in the Compendium of Material Medica, OVIDUCTUS RANNAE is warm in nature, returns lung, kidney channel, has tonifying kidney and benefiting sperm, Yin nourishing and lung moistening, qi-restoratives effect such as bring down a fever.OVIDUCTUS RANNAE is in the product recovery process, and part frog is dried goes out of use for other of frog body, and modern check and analysis show, the frog is dried to be high-quality and cheap protein, origin of amino acid, and contains a large amount of collagens, is to mend the splendid dietary protein origin of iron.Iron is one of necessary trace element of people, and it has the physiological action of hematopoiesis and oxygen therapy gas, also is the enzyme wanted of body weight for humans and the constituent of albumen, and the health of human body is had important function.Ferroheme is synthetic during the human body iron deficiency reduces, and the red blood cell hypochrosis microcytic anemia takes place, i.e. hypoferric anemia, and the iron content enzymatic activity reduces in the body simultaneously, influences energetic supersession.The shortage of iron is all more general than the shortage of other any nutrients, is one and is with one of global nutrition problem.According to estimates, the whole world has 10%～25% population to be subjected to sideropenic threat, those food supply deficiencies particularly, and the countries and regions that living standard falls behind are common especially.Hypoferric anemia can betide the people at any age, especially with the easiest generations of crowd such as the children (especially premature) that were born 6 months to 6 years old, children in adolescence, pregnant woman, parasitic infections.The generaI investigation of China part provinces and cities is found 7 years old following Iron deficiency anemia in children illness rate up to 57.6%, and is especially the highest with 1～3 years old infant.In people's growth and development process, infant, children, puberty are the periods that nutrition needs most, and iron is a nutrient that merits attention very much.Iron main ferric ion form with indissoluble in natural environment exists, and can not directly be absorbed by body, needs special movement system be absorbed into blood, and transports in blood with soluble form.The iron that enters in the body is absorbed at small intestine, and its absorption site mainly is the cell mucous membrane of duodenum and jejunum epimere.Chinese scholars thinks that by a series of researchs iron must combine with endogenous or external source ligand and form the huge legendary turtle compound in the enteric cavity of human body, absorb with this coordination huge legendary turtle compound form and further transhipment, and amino acid and polypeptide are exactly the main body of these ligands again.

Summary of the invention

Purpose of the present invention just provides a kind of production method of wood frog iron supplement capsules, by utilizing complex polypeptide that the dried enzymolysis of the wood frog discarded object frog obtains and amino acid and molysite to carry out chelatropic reaction, make the polypeptide chelate molysite, reach the scope of application that enlarges the frog and do, the purpose that turns waste into wealth, improves iron absorption rate in human body.

The object of the present invention is achieved like this: a kind of production method of wood frog iron supplement capsules, and this method may further comprise the steps:

(1) drying: the dried ventilation drying baker of gathering of putting into of the frog is dried to constant weight for 50～60 ℃;

(2) pulverize: be crushed to 20～40 orders;

(3) homogenate: with the dried soak at room temperature of the frog pulverized 3 hours, add 3～5 times water homogenate again with the pure water of 3～5 times of volumes;

(4) enzymolysis: use the papain 10～20 of 60U/g, the neutral proteinase 10～20 of 12U/g, under temperature 45-60 ℃, the condition of pH value 5-7 enzymolysis 2-4 hour, make it resolve into polypeptide and amino acid etc., heat to 10min more than 80 ℃, inactivated proteases is filtered, the preparation enzymolysis liquid;

(5) chelating: with FeCl ₂Be source of iron, be 5.0～6.0 at pH value, polypeptide-weight of iron ratio is to stir in 3～4: 1 the reactant liquor, behind the chelating 30min, join in the above-mentioned reactant liquor with absolute ethyl alcohol and to precipitate, the ratio of its absolute ethyl alcohol and reactant liquor is 1: 8～10;

(6) drying: leave standstill after 12 hours and filter, with above-mentioned wood frog polypeptide, amino acid chelated iron air dry or place freeze drying in the freeze drier, the freeze-drying parameter is: pre-freeze temperature-35 ℃～-32 ℃, material thickness 2cm, vacuum 60～100Pa obtains peptide and the iron of moisture content≤5-18% after the drying;

(7) perfusion capsule: behind compatibility VC, the folic acid etc., pour in the capsule irradiation sterilization.

Good effect of the present invention is to have made full use of the peculiar wood frog frog of China and does resource; use zymolysis technique and produce compound amino acid, complex polypeptide etc.; compatibility is produced produce effects deep processed products such as " wood frog iron supplement capsules "; guaranteed the sustainable utilization of wood frog resource; turn waste into wealth; also protect ecological environment, and promoted iron in human body, to absorb, had reasonable simple, the dried high characteristics of utilization rate of the frog of technology.

The specific embodiment

Give an actual example below and describe the present invention.A kind of production method of wood frog iron supplement capsules, this method may further comprise the steps:

(1) drying: the dried ventilation drying baker of gathering of putting into of the frog is dried to constant weight for 55 ℃;

(2) pulverize: be crushed to 30 orders;

(3) homogenate: with the dried soak at room temperature of the frog pulverized 3 hours, add 4 times water homogenate again with the pure water of 4 times of volumes;

(4) enzymolysis: use the papain 15 of 60U/g, the neutral proteinase 15 of 12U/g, enzymolysis is 3 hours under the condition of 50 ℃ of temperature, pH value 6, makes it resolve into polypeptide and amino acid etc., heat to 10min more than 80 ℃, inactivated proteases is filtered, the preparation enzymolysis liquid;

(5) chelating: with FeCl ₂Be source of iron, be 5.5 at pH value, polypeptide-weight of iron ratio is to stir in 3.5: 1 the reactant liquor, behind the chelating 30min, join in the above-mentioned reactant liquor with absolute ethyl alcohol and to precipitate, the ratio of its absolute ethyl alcohol and reactant liquor is 1: 9;

(6) drying: leave standstill after 12 hours and filter, with above-mentioned wood frog polypeptide, amino acid, chelated iron air dry or place freeze drying in the freeze drier, the freeze-drying parameter is: pre-freeze temperature-33 ℃, material thickness 2cm, vacuum 80Pa obtains peptide and the iron of moisture content≤5-18% after the drying;

(7) perfusion capsule: behind compatibility VC, the folic acid etc., pour in the capsule irradiation sterilization.

Claims (1)

1, a kind of production method of wood frog iron supplement capsules is characterized in that this method may further comprise the steps:

(1) drying: the dried ventilation drying baker of gathering of putting into of the frog is dried to constant weight for 50～60 ℃;

(2) pulverize: be crushed to 20～40 orders;

(3) homogenate: with the dried soak at room temperature of the frog pulverized 3 hours, add 3～5 times water homogenate again with the pure water of 3～5 times of volumes;

(4) enzymolysis: use the papain 10～20 of 60U/g, the neutral proteinase 10～20 of 12U/g, under temperature 45-60 ℃, the condition of pH value 5-7 enzymolysis 2-4 hour, make it resolve into polypeptide and amino acid, heat to 10min more than 80 ℃, inactivated proteases is filtered, the preparation enzymolysis liquid;

(5) chelating: with FeCl ₂Be source of iron, be 5.0～6.0 at pH value, polypeptide-weight of iron ratio is to stir in 3～4: 1 the reactant liquor, behind the chelating 30min, join in the above-mentioned reactant liquor with absolute ethyl alcohol and to precipitate, the ratio of its absolute ethyl alcohol and reactant liquor is 1: 8～10;

(6) drying: leave standstill after 12 hours and filter, with above-mentioned wood frog polypeptide, amino acid chelated iron air dry or place freeze drying in the freeze drier, the freeze-drying parameter is: pre-freeze temperature-35 ℃～-32 ℃, material thickness 2cm, vacuum 60～100Pa obtains peptide and the iron of moisture content≤5-18% after the drying;

(7) perfusion capsule: behind compatibility VC, the folic acid, pour in the capsule irradiation sterilization.