CN102038077A - Secondary enzymolysis method for preparing heme iron for feeds - Google Patents
Secondary enzymolysis method for preparing heme iron for feeds Download PDFInfo
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- CN102038077A CN102038077A CN2010102812068A CN201010281206A CN102038077A CN 102038077 A CN102038077 A CN 102038077A CN 2010102812068 A CN2010102812068 A CN 2010102812068A CN 201010281206 A CN201010281206 A CN 201010281206A CN 102038077 A CN102038077 A CN 102038077A
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Abstract
The invention relates to a secondary enzymolysis method for preparing heme iron for feeds, which comprises the following steps: acquiring fresh animal blood, carrying out anticoagulation, centrifugating to obtain blood corpuscles, adding 1-4 times by weight of water to the blood corpuscles, stirring for 1-3 hours to break walls, keeping the temperature at 50-55 DEG C, adding protease to carry out enzymolysis, regulating the pH value, carrying out pressure filtration, collecting filter cakes, dissolving, carrying out secondary enzymolysis, collecting filter cakes, and drying with a flash evaporation drying machine. The heme iron obtained by the method in the invention has the characteristics of high heme iron content and high absorptivity.
Description
Technical field:
The present invention relates to a kind of feed addictive preparation technology, particularly a kind of feed secondary enzymolysis preparation method of heme iron.
Background technology:
Pig blood is a kind of nutritive value that can obtain in a large number in butchering process and the very high industry byproduct of biological value.Nearly 500,000,000 of live pig is butchered every year by China, account for world's amount of butchering near half, the annual total output of blood resource reaches ten thousand tons of 250-300, this abundant animal blood resource except that sub-fraction is used for food industry and feed industry, is mostly thrown away as discarded object.This not only causes the serious waste of resource, and causes river, the environmental pollution of underground water ammonia nitrogen.From the exploitation of new edible protein resource, the purposes such as effective utilization, the pollution of minimizing slaughterhouse and raising business economic benefit of accessory substance, the further utilization of livestock and poultry blood is very necessary.
Iron is the essential nutrient of body in the adults body, and the body iron deficiency can cause trophism microcytic cell anaemia (hypoferric anemia), and influences the immunity of body.The iron that body needs is from food, and the existence form of iron in food mainly contains two classes: a class is a nonheme iron, and from plant food, absorptivity is lower; Another kind of is heme iron, from animal food.Because heme iron is not suppressed the influence of factor, so absorptivity is than the nonheme iron height.Contain abundant iron in the pig blood, iron-holder is up to 45 milligrams in every hectogram, and that the iron ion in the pig blood nearly all is the ferrous iron that very easily is absorbed by the body is identical with the chemical valence of iron ion in the human body, all is the hemochrome sections, the utilization that can directly be absorbed by the body after ingesting, absorptivity can reach 30%.Heme iron can be separated from blood and protected heme iron that can be stable by certain method.Heme iron as iron-supplementing preparation have concurrently absorptivity height, side effect lower, easily absorb and advantage such as do not stimulate digestion the raw material of pharmacy product such as still antitumor simultaneously, coloring agent and treatment lead poisoning and food additives.
Since century, lot of domestic and foreign scientific research personnel is round this problem of extraction of heme iron, in continuous exploration more than one.The report of relevant heme iron extracting method is a lot, comprising: ice acetic acid method, acid acetone method, CMC absorption method, soda acid purifying extraction method, surfactant method, selective solvent method, base thing one organic solvent method, pure method, acetify one Hydrolyze method, enzyme extraction method.
Enzyme extraction method utilizes protease that globin in the hemoglobin is hydrolyzed into water soluble amino-acid and polypeptide, supernatant is a soluble polypeptide, and it is precipitated as heme iron, and water-fast heme iron is separated, through washing, filtration, drying, make powdery product.The protease hydrolytic method is not only separated protein and ferroheme, can also improve the rate that digests and absorbs of hemoglobin, is beneficial to utilize respectively, is a kind of approach that utilizes preferably.Enzymatic isolation method has not only improved the yield and the purity of product, the more important thing is and in the extraction of heme iron and purge process, avoid higher and bigger chloroform and the pyridine solvent of toxicity of use cost, reduced production cost, and can avoid acid, defectives such as production cycle length, etching apparatus, contaminated environment during the basic hydrolysis hemoglobin, enlarged the range of application of hydrolysate, reduce environmental pollution, be considered to a kind of method of extraction heme iron of environmental protection.But the ferroheme purity that enzymatic isolation method makes also be not very high, iron content is lower, absorptivity is not high yet.
Summary of the invention:
The purpose of this invention is to provide a kind of feed with heme iron preparation and improve the method for its heme iron content, the product that this method makes has iron content height, characteristics that absorptivity is high.
As above design, technical scheme of the present invention is: a kind of feed secondary enzymolysis preparation method of heme iron is characterized in that: finish according to the following step:
(1) separation of blood cell and broken wall: gather fresh animal blood, carry out centrifugation after the adding anti-coagulants fully mixes and obtain blood cell liquid, separate obtaining blood cell liquid, in blood cell liquid, add water stirring carrying out blood cell broken wall;
(2) primary enzymolysis: add 4 ‰-6 ‰ protease in above-mentioned blood cell liquid, 50-55 ℃ of constant temperature hydrolysis 10-16 hour, stops hydrolysis;
(3) filter cake on the filter cloth is collected in press filtration;
(4) secondary enzymolysis: with adding filter cake weight 5-10 water-soluble separating doubly in the above-mentioned filter cake, add 2 ‰-3 ‰ protease in the solution, 50-55 ℃ of constant temperature hydrolysis 10-16 hour, stops hydrolysis;
(5) filter cake on the filter cloth is collected in press filtration;
(6) drying.
Above-mentioned anti-coagulants employing mass fraction is 10% natrium citricum.
Above-mentioned centrifugal condition is: the centrifugal 5～15min of 3500～7000r/min rotating speed.
The condition of above-mentioned blood cell broken wall is: add blood cell weight 1-4 water doubly in blood cell, stirred 1-3 hour.
Above-mentioned protease adopts animal proteolytic enzyme.
The condition of above-mentioned press filtration is: enzymolysis liquid is transferred to pH5-5.5 with 4-6mol/L hydrochloric acid, import plate and frame filter press then through 800 order filter cloth press filtrations.
The above-mentioned dry flash dryer that adopts.
The invention has the advantages that:
1, this method simple to operate, be easy to suitability for industrialized production, cost is lower.
2, the heme iron content of product is 1.5 times of primary enzymolysis product heme iron content after the secondary enzymolysis.
3, prepared product heme iron content height.
4, prepared product absorptivity height.
The specific embodiment:
Get fresh pig blood, adding mass fraction and be 10% natrium citricum, to make the content of its natrium citricum be 4.5 ‰; With tube centrifuge separated plasma and blood cell, centrifugal condition is: the centrifugal 5～15min of 3500～7000r/min rotating speed.Get blood cell liquid, in blood cell liquid, add the water of 2 times of volumes, start and stirred 1-3 hour, carry out the blood cell fragmentation; Behind the broken wall blood cell liquid is warming up to 55 ℃, adds 5 ‰ animal proteolytic enzymes, enzymolysis is after 12 hours, transfer pH5.0 with 6mol/L hydrochloric acid, enzymolysis liquid carries out press filtration by filter press, collects the filter cake on the filter cloth, add 5 times of water-soluble separating, be warming up to 55 ℃, add 2.5 ‰ animal proteolytic enzyme, enzymolysis is after 10 hours, 6mol/L hydrochloric acid is transferred pH5.0, enzymolysis liquid carries out press filtration by filter press, collects filter cake, flash dryer.
Claims (7)
1. a feed is characterized in that: finish according to the following step with the secondary enzymolysis preparation method of heme iron:
(1) separation of blood cell and broken wall: gather fresh animal blood, carry out centrifugation after the adding anti-coagulants fully mixes and obtain blood cell liquid, separate obtaining blood cell liquid, in blood cell liquid, add water stirring carrying out blood cell broken wall;
(2) primary enzymolysis: add 4 ‰-6 ‰ protease in above-mentioned blood cell liquid, 50-55 ℃ of constant temperature hydrolysis 10-16 hour, stops hydrolysis;
(3) filter cake on the filter cloth is collected in press filtration;
(4) secondary enzymolysis: with adding filter cake weight 5-10 water-soluble separating doubly in the above-mentioned filter cake, add 2 ‰-3 ‰ protease in the solution, 50-55 ℃ of constant temperature hydrolysis 10-16 hour, stops hydrolysis;
(5) filter cake on the filter cloth is collected in press filtration;
(6) drying.
2. the feed according to claim 1 secondary enzymolysis preparation method of heme iron is characterized in that: above-mentioned anti-coagulants employing mass fraction is 10% natrium citricum.
3. the feed according to claim 1 secondary enzymolysis preparation method of heme iron, it is characterized in that: above-mentioned centrifugal condition is: the centrifugal 5～15min of 3500～7000r/min rotating speed.
4. the feed according to claim 1 secondary enzymolysis preparation method of heme iron, it is characterized in that: the condition of above-mentioned blood cell broken wall is: add blood cell weight 1-4 water doubly in blood cell, stirred 1-3 hour.
5. feed according to claim 1 is characterized in that: above-mentioned protease employing animal proteolytic enzyme with the secondary enzymolysis preparation method of heme iron.
6. the feed according to claim 1 secondary enzymolysis preparation method of heme iron, it is characterized in that: the condition of above-mentioned press filtration is: enzymolysis liquid is transferred pH5-5.5 with 4-6mol/L hydrochloric acid, import plate and frame filter press, through 800 order filter cloth press filtrations.
7. feed according to claim 1 is characterized in that: the above-mentioned dry flash dryer that adopts with the secondary enzymolysis preparation method of heme iron.
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| CN2010102812068A CN102038077A (en) | 2010-09-14 | 2010-09-14 | Secondary enzymolysis method for preparing heme iron for feeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030645A (en) * | 2012-12-21 | 2013-04-10 | 上海杰隆生物制品股份有限公司 | Method for preparing high-purity heme on large scale and application of heme |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN106107574A (en) * | 2016-08-11 | 2016-11-16 | 安徽省农业科学院农产品加工研究所 | A kind of preparation method of Sanguis sus domestica source compound microelement supplement |
| CN106497997A (en) * | 2016-11-18 | 2017-03-15 | 安徽菁硕科技有限公司 | A kind of method that haemachrome is produced by the enzymatic isolation method that interlocks |
| CN109675071A (en) * | 2019-01-26 | 2019-04-26 | 湖南璟荣生物科技有限公司 | A kind of sterilized method of animal blood |
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| CN1300732A (en) * | 2000-12-28 | 2001-06-27 | 内蒙古草原兴发股份有限公司 | Process for extracting heme from animal blood |
| KR20060005770A (en) * | 2004-07-14 | 2006-01-18 | 주식회사 에이.비.아이 | Preparation of water soluble heme-iron polypeptide complex from animal whole blood or clotted blood |
| CN101623043A (en) * | 2009-02-26 | 2010-01-13 | 天津宝迪农业科技股份有限公司 | Technology using swine fresh pancreatin to produce swine blood protein peptide and haemoglobin |
| CN101649342A (en) * | 2009-09-02 | 2010-02-17 | 张滨 | Purifying method of bivalent heme polypeptide-ferrum and application thereof |
| CN101748181A (en) * | 2010-01-15 | 2010-06-23 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
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| CN1300732A (en) * | 2000-12-28 | 2001-06-27 | 内蒙古草原兴发股份有限公司 | Process for extracting heme from animal blood |
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| CN101748181A (en) * | 2010-01-15 | 2010-06-23 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030645A (en) * | 2012-12-21 | 2013-04-10 | 上海杰隆生物制品股份有限公司 | Method for preparing high-purity heme on large scale and application of heme |
| CN103030645B (en) * | 2012-12-21 | 2016-07-06 | 上海杰隆生物制品股份有限公司 | A kind of method of extensive preparation high-purity haemachrome and application |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN106107574A (en) * | 2016-08-11 | 2016-11-16 | 安徽省农业科学院农产品加工研究所 | A kind of preparation method of Sanguis sus domestica source compound microelement supplement |
| CN106497997A (en) * | 2016-11-18 | 2017-03-15 | 安徽菁硕科技有限公司 | A kind of method that haemachrome is produced by the enzymatic isolation method that interlocks |
| CN109675071A (en) * | 2019-01-26 | 2019-04-26 | 湖南璟荣生物科技有限公司 | A kind of sterilized method of animal blood |
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Application publication date: 20110504 |