CN101480254B - Method for producing peptide iron powder using pig blood - Google Patents
Method for producing peptide iron powder using pig blood Download PDFInfo
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- CN101480254B CN101480254B CN200910013887A CN200910013887A CN101480254B CN 101480254 B CN101480254 B CN 101480254B CN 200910013887 A CN200910013887 A CN 200910013887A CN 200910013887 A CN200910013887 A CN 200910013887A CN 101480254 B CN101480254 B CN 101480254B
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Abstract
The invention discloses a method for producing polypeptide-fe by pig blood, which belongs to the field of the deep processing and utilizing process and comprises processes of pretreatment, separation, pancreas treatment, fracture, zymohydrolysis, complexation, bath, drying, and the like. The invention can prepare polypeptide-fe which is favorable to absorbing and can be used as raw materials or additive of various foods for using.
Description
Technical field
The present invention utilizes pig blood to produce the method and the product of peptide iron powder, the production technology field of be born in the year of pig blood deep processing, utilization.
Background technology
Containing abundant heme iron in the pig blood, be a kind of non-defective unit of enriching blood, but iron wherein and protein combination is tight, and complexing is the big compound of molecular weight, is not easy to absorb, and does not add the effect of handling inaccessible benefit iron behind the direct food prepared therefrom.
Summary of the invention
The purpose of this invention is to provide the method for utilizing pig blood to produce peptide iron powder, can extract the iron that reclaims in the pig blood efficient, safely cheaply.
For reaching above purpose, the technical scheme that the present invention takes is: 1, preliminary treatment: adopt fresh pig blood, add 3.8% natrium citricum 8%, stir; 2, separate: with pig blood under the 5000r/min rotating speed centrifugal 6 minutes, with serum and separating plasma, it was subsequent use to put into refrigerator; 3, pancreas is handled: get fresh pancreas, remove fat, smash, get 30g and add the 3ml absolute ethyl alcohol, stir, placed 3-4 hour; 4, break: get the blood plasma after the separation, add the distilled water of 2.5 times of volumes, stirred 15 minutes, haemocyte is broken; 5, enzymolysis: the haemocyte that will break is regulated pH to 8.5 with 10% NaOH solution, stirs, and in 30-50 ℃ of water-bath, adds the pancreas after activating; Reacted 4-5 hour; Beginning was whenever stirred once at a distance from 15 minutes, and stir once every separated half an hour after two hours, stirred 2-3 minute at every turn; 6, complexing: rapidly bath temperature is increased to 90 ℃, deactivation 15 minutes is cooled in half an hour below 40 ℃; Regulate pH to 4-6 with 10% hydrochloric acid solution; Add chitosan solution, chitosan solution is dissolved in for the 4g shitosan in the water of 200-500ml, and the limit edged stirs; React the NaOH solution of adding 10% after 15 minutes and regulate pH to 7, sedimentation and filtration; 7, washing: filter residue is with washing 3-5 time, and to the washing lotion clarification, deposition is the wet article of peptide iron.The wet article of peptide iron are carried out 40-50 ℃ low-temperature vacuum drying,, sieve and be peptide iron powder through pulverizing.
The present invention has the following advantages: 1, remarkable benefit, the iron in the pig blood is extracted, and complexing makes iron more be prone to be absorbed again, can be used as the raw material of various benefit iron products, remarkable benefit.2, with low cost, the used most of raw material of the present invention is common materials.3, safety non-toxic, material therefor is food-grade.
The specific embodiment
Specify production process of the present invention, 1, preliminary treatment: adopt fresh pig blood, add 3.8% natrium citricum 8%, stir at present; 2, separate: with pig blood under the 5000r/min rotating speed centrifugal 6 minutes, with serum and separating plasma, it was subsequent use to put into refrigerator; 3, pancreas is handled: get fresh pancreas, remove fat, smash, get 30g and add the 3ml absolute ethyl alcohol, stir exquisite 3-4 hour; 4, break: get the blood plasma after the separation, add the distilled water of 2.5 times of volumes, stirred 15 minutes, haemocyte is broken; 5, enzymolysis: the haemocyte that will break is regulated pH to 8.5 with 10% NaOH solution, stirs, and in 30-50 ℃ of water-bath, adds the pancreas after activating; Reacted 45 hours; Beginning was whenever stirred once at a distance from 15 minutes, and stir once every separated half an hour after two hours, stirred 2-3 minute at every turn; 6, complexing: rapidly bath temperature is increased to 90 ℃; Deactivation 15 minutes; Be cooled to 40 ℃ in half an hour once, regulate pH to 4-6, add shitosan (4g is dissolved in the 200-500ml water) limit edged and stir with 10% hydrochloric acid solution; React the NaOH solution of adding 10% after 15 minutes and regulate pH to 7, sedimentation and filtration; 7, washing: filter residue is with water system 3-5 time, to the washing lotion clarification, precipitates the peptide iron article that wet that are.The wet article of peptide iron are carried out low-temperature vacuum drying (40-50 ℃), and through pulverizing, screening is peptide iron powder.
Claims (2)
1. utilize pig blood to produce the method for peptide iron powder, it is characterized in that: 1, preliminary treatment: adopt fresh pig blood, add 3.8% natrium citricum 8%, stir; 2, separate: with pig blood under the 5000r/min rotating speed centrifugal 6 minutes, with serum and separating plasma, it was subsequent use to put into refrigerator; 3, pancreas is handled: get fresh pancreas, remove fat, smash, get 30g and add the 3ml absolute ethyl alcohol, stir, placed 3-4 hour; 4, break: get the blood plasma after the separation, add the distilled water of 2.5 times of volumes, stirred 15 minutes, haemocyte is broken; 5, enzymolysis: the haemocyte that will break is regulated pH to 8.5 with 10% NaOH solution, stirs, and in 30-50 ℃ of water-bath, adds the pancreas after activating; Reacted 4-5 hour; Beginning was whenever stirred once at a distance from 15 minutes, and stir once every separated half an hour after two hours, stirred 2-3 minute at every turn; 6, complexing: rapidly bath temperature is increased to 90 ℃, deactivation 15 minutes is cooled in half an hour below 40 ℃; Regulate pH to 4-6 with 10% hydrochloric acid solution; Add chitosan solution, chitosan solution is dissolved in for the 4g shitosan in the water of 200-500ml, and the limit edged stirs; React the NaOH solution of adding 10% after 15 minutes and regulate pH to 7, sedimentation and filtration; 7, washing: filter residue is with washing 3-5 time, and to the washing lotion clarification, deposition is the wet article of peptide iron.
2. according to the said method of utilizing pig blood to produce peptide iron powder of claims 1, it is characterized in that: the wet article of peptide iron are carried out 40-50 ℃ low-temperature vacuum drying,, sieve and be peptide iron powder through pulverizing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910013887A CN101480254B (en) | 2009-01-22 | 2009-01-22 | Method for producing peptide iron powder using pig blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910013887A CN101480254B (en) | 2009-01-22 | 2009-01-22 | Method for producing peptide iron powder using pig blood |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101480254A CN101480254A (en) | 2009-07-15 |
| CN101480254B true CN101480254B (en) | 2012-09-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910013887A Expired - Fee Related CN101480254B (en) | 2009-01-22 | 2009-01-22 | Method for producing peptide iron powder using pig blood |
Country Status (1)
| Country | Link |
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| CN (1) | CN101480254B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982095B (en) * | 2010-09-09 | 2013-01-09 | 得利斯集团有限公司 | Peptide iron capsule and preparation method thereof |
| CN104012839B (en) * | 2014-05-19 | 2016-04-06 | 中国人民解放军军事医学科学院基础医学研究所 | Healthcare products preparation chitosan-polypeptide complex and preparation method thereof |
| CN106520882A (en) * | 2016-12-10 | 2017-03-22 | 广州市嘉仁高新科技有限公司 | Production method of extracting small-peptide ferroheme from animal blood |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185920A (en) * | 1996-12-25 | 1998-07-01 | 范启修 | Blood enriching food |
| CN1362525A (en) * | 2001-01-02 | 2002-08-07 | 蒋佃水 | Small peptide heme and its prepn |
| CN1843147A (en) * | 2006-03-02 | 2006-10-11 | 四川大学 | Short peptide protein powder preparation method using livestock blood as material |
-
2009
- 2009-01-22 CN CN200910013887A patent/CN101480254B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185920A (en) * | 1996-12-25 | 1998-07-01 | 范启修 | Blood enriching food |
| CN1362525A (en) * | 2001-01-02 | 2002-08-07 | 蒋佃水 | Small peptide heme and its prepn |
| CN1843147A (en) * | 2006-03-02 | 2006-10-11 | 四川大学 | Short peptide protein powder preparation method using livestock blood as material |
Non-Patent Citations (2)
| Title |
|---|
| 姚汉亭等.猪血水解混合氨基酸强化面粉对大鼠生长的影响.《食品科学》.1987,(第01期), * |
| 孙骞等.血红蛋白酶法脱色技术研究.《肉类研究》.2008,(第11期), * |
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| CN101480254A (en) | 2009-07-15 |
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Effective date of registration: 20171016 Address after: 518000, Guangdong, Shenzhen province Binhe Road, Futian District City Golf (phase two) green seat 11 layer G Patentee after: Shenzhen Delisi Trade Co. Ltd. Address before: 262216 Shandong province Weifang city Zhucheng Town Industrial Park Chang Idris Delisi Group Ltd Patentee before: Delicious Group Co., Ltd. |
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