CN108850625B - Calcium deficiency type disease mouse model feed and preparation method thereof - Google Patents
Calcium deficiency type disease mouse model feed and preparation method thereof Download PDFInfo
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- 206010006956 Calcium deficiency Diseases 0.000 title claims abstract description 27
- 238000010172 mouse model Methods 0.000 title claims abstract description 22
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 20
- 201000010099 disease Diseases 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title abstract description 11
- 239000011575 calcium Substances 0.000 claims abstract description 20
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 20
- 235000016709 nutrition Nutrition 0.000 claims abstract description 20
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 19
- 240000008042 Zea mays Species 0.000 claims abstract description 19
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 19
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 19
- 235000005822 corn Nutrition 0.000 claims abstract description 19
- 230000035764 nutrition Effects 0.000 claims abstract description 18
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 17
- 239000011707 mineral Substances 0.000 claims abstract description 17
- 239000005018 casein Substances 0.000 claims abstract description 15
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000021240 caseins Nutrition 0.000 claims abstract description 15
- 239000003623 enhancer Substances 0.000 claims abstract description 15
- 244000068988 Glycine max Species 0.000 claims abstract description 12
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 8
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 8
- 229920002472 Starch Polymers 0.000 claims abstract description 8
- 239000001913 cellulose Substances 0.000 claims abstract description 8
- 229920002678 cellulose Polymers 0.000 claims abstract description 8
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229960001231 choline Drugs 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 8
- 235000009566 rice Nutrition 0.000 claims abstract description 8
- 239000004455 soybean meal Substances 0.000 claims abstract description 8
- 235000012424 soybean oil Nutrition 0.000 claims abstract description 8
- 239000003549 soybean oil Substances 0.000 claims abstract description 8
- 239000008107 starch Substances 0.000 claims abstract description 8
- 235000019698 starch Nutrition 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000011782 vitamin Substances 0.000 claims abstract description 6
- 229940088594 vitamin Drugs 0.000 claims abstract description 6
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 8
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 8
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 8
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 235000019155 vitamin A Nutrition 0.000 claims description 8
- 239000011719 vitamin A Substances 0.000 claims description 8
- 239000011716 vitamin B2 Substances 0.000 claims description 8
- 239000011726 vitamin B6 Substances 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- 229940045997 vitamin a Drugs 0.000 claims description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 239000011686 zinc sulphate Substances 0.000 claims description 8
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 7
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 7
- 229910052603 melanterite Inorganic materials 0.000 claims description 7
- 239000011647 vitamin D3 Substances 0.000 claims description 7
- 239000011652 vitamin K3 Substances 0.000 claims description 7
- 241000700159 Rattus Species 0.000 claims description 6
- 230000002950 deficient Effects 0.000 claims description 6
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 claims description 5
- 239000011691 vitamin B1 Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 7
- 235000019629 palatability Nutrition 0.000 abstract description 5
- 208000020084 Bone disease Diseases 0.000 abstract description 4
- 231100000915 pathological change Toxicity 0.000 abstract description 4
- 230000036285 pathological change Effects 0.000 abstract description 4
- 241000699670 Mus sp. Species 0.000 description 12
- 238000010521 absorption reaction Methods 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 4
- 229930003270 Vitamin B Natural products 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011720 vitamin B Substances 0.000 description 3
- 235000019156 vitamin B Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229930003316 Vitamin D Natural products 0.000 description 2
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000011710 vitamin D Substances 0.000 description 2
- 235000019166 vitamin D Nutrition 0.000 description 2
- 150000003710 vitamin D derivatives Chemical class 0.000 description 2
- 229940046008 vitamin d Drugs 0.000 description 2
- 206010001497 Agitation Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000004578 fetal growth Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229910001428 transition metal ion Inorganic materials 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
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- Zoology (AREA)
- Food Science & Technology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Biotechnology (AREA)
- Botany (AREA)
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Abstract
The invention discloses a calcium deficiency type disease mouse model feed and a preparation method thereof. The feed comprises the following components in percentage by weight: 5 to 6 percent of casein, 5 to 6 percent of wheat bran, 27 to 29 percent of corn, 11 to 12 percent of soybean meal, 3 to 4 percent of soybean oil, 12 to 13 percent of soybean, 18 to 19 percent of rice, 7 to 8 percent of starch, 1 to 2 percent of cellulose, 0.5 to 1 percent of mineral nutrition enhancer, 0.1 to 0.5 percent of Chinese multi-vitamin, 0.05 to 0.1 percent of choline and the balance of CaHPO4·2H2O; the preparation method comprises the following steps: the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases. The invention can improve the palatability of the feed and reduce the feed cost, and can be applied to the establishment of bone disease models caused by low calcium of patients in medical experiments and the research of other pathological changes caused by calcium deficiency.
Description
Technical Field
The invention belongs to the technical field of feed preparation, and particularly relates to a calcium deficiency type disease mouse model feed and a preparation method thereof.
Background
Calcium is a mineral element with the largest content in human bodies, and mainly exists in teeth and bones, and also exists in a small amount in body tissues and blood. Calcium plays an important role in the normal growth of bones and in maintaining the excitability of nerve cells, and is also involved in the blood coagulation process. The lack of calcium in human food can cause deformation of bones and slow growth, rickets appear, the fetal growth can be affected due to the lack of calcium in pregnancy, postpartum paralysis can occur due to long-term lack, and the absorption and metabolism of various nutrients are also affected by the calcium. For example, in addition to the well-known phosphorus and vitamin D, other nutrients such as amino acids, fatty acids, magnesium, zinc, iodine, iron, manganese, copper, etc. interact with calcium. Therefore, the biological role of calcium is studied or a calcium deficiency model is reproduced, and the feed used is very critical to the results of the study.
At the present stage, the domestic diet for calcium-deficient rats and mice generally refers to a formula in foreign documents, and the foreign countries generally adopt a full-purity daily ration to obtain the low-calcium rat and mouse feed, so that on one hand, the palatability of the pure daily ration is poor, animals are not easy to accept, on the other hand, the cost is high, and the raw materials are easy to counterfeit.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a calcium deficiency type disease mouse model feed and a preparation method thereof, which can effectively solve the problems of poor palatability, high cost and insufficient nutrient components of the existing feed.
A calcium deficiency type disease mouse model feed comprises the following components in percentage by weight:
5 to 6 percent of casein, 5 to 6 percent of wheat bran, 27 to 29 percent of corn, 11 to 12 percent of soybean meal, 3 to 4 percent of soybean oil, 12 to 13 percent of soybean, 18 to 19 percent of rice, 7 to 8 percent of starch, 1 to 2 percent of cellulose, 0.5 to 1 percent of mineral nutrition enhancer, 0.1 to 0.5 percent of Chinese multi-vitamin, 0.05 to 0.1 percent of choline and the balance of CaHPO4·2H2O。
Further, the paint comprises the following components in percentage by weight:
6% of casein, 6% of wheat bran, 28.78% of corn, 12% of soybean meal, 4% of soybean oil, 13% of soybean, 19% of rice, 8% of starch, 2% of cellulose, 1% of mineral nutrition enhancer, 0.1% of Chinese multivitamin, 0.06% of choline and the balance of CaHPO4·2H2O。
Further, the corn is GB1 grade corn.
Further, the soybean is NY/T2 grade soybean.
Further, the mineral nutrition enhancer comprises the following components in percentage by weight:
FeSO4·7H2O 19％～20％、MnSO4·H2O 31％～32％、ZnSO4·7H2O 22％～23％、CuSO4·5H225-26% of O, 3-4% of KI and the balance of corn flour.
Further, the mineral nutrition enhancer comprises the following components in percentage by weight:
FeSO4·7H2O 19.7％、MnSO4·H2O 31.8％、ZnSO4·7H2O 22.5％、CuSO4·5H2o25%, KI 3.8% and the balance of corn flour.
Further, the Chinese multi-dimensional food comprises the following components:
vitamin A and vitamin B1Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3(ii) a Wherein, vitamin A and vitamin B1Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3The weight ratio of (1: 0.5:1.5:0.8:0.3:1.4: 1.1).
The preparation method of the calcium deficiency type disease mouse model feed comprises the following steps:
the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases.
The invention has the beneficial effects that:
1. the casein can be used as a thickening agent of the feed while enhancing the nutrition of the feed, if the content of the casein is too high, the cost of the feed can be improved, the palatability of the feed can be reduced, the addition amount of the casein in the formula is reduced, Chinese multivitamins in the feed and components in a mineral nutrition enhancer are combined, the palatability of the feed can be effectively improved, the cost of the feed is reduced, and the nutritional value of the feed is ensured; meanwhile, each kilogram of daily ration in the formula designed by the invention contains 0.905g of calcium, which is lower than the standard that each kilogram of daily ration of rats and mice in experiments required in the national standard GB14924.3-2010 contains 10g, and the formula can be applied to the establishment of bone disease models caused by low calcium of patients in medical experiments and the research of other pathological changes caused by calcium deficiency.
2. Casein is a large, hard and compact curd which is extremely difficult to digest and decompose in animals and human bodies, and FeSO is added into the formula to facilitate the digestion and absorption of the casein in mice4·7H2O、MnSO4·H2O、ZnSO4·7H2O、CuSO4·5H2O, the metal ions generate chelation with casein in the mouse body to form a transition metal ion chelate, which not only promotes the absorption of casein by the mouse, but also promotes the decomposition and absorption of various mineral substances by the mouse,the purpose of improving the nutritive value of the feed is achieved.
3. When a plurality of metal ions are chelated with the amino acid at the same time, a competitive relationship exists among the metal ions, through the mutual competitive inhibition among the metal ions, the chelating amount of the calcium ions and the amino acid can be reduced, and the absorption of the calcium ions by the mouse is effectively hindered on the premise of ensuring the nutrition of the mouse.
4. Various vitamins added in the formula can effectively improve the nutritional value of the formula, promote the growth and development of mice, improve the immunocompetence of the mice and strengthen the physique of the mice; at the same time, vitamin B1Vitamin B2And vitamin B6Can synergistically promote the absorption of vitamin C, vitamin A and vitamin D in mice3The components also have the function of mutual cooperation and absorption promotion.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and it will be apparent to those skilled in the art that various changes may be made without departing from the spirit and scope of the invention as defined and defined in the appended claims, and all matters produced by the invention using the inventive concept are protected.
Example 1
A calcium deficiency type disease mouse model feed comprises the following components in percentage by weight:
casein 5%, wheat bran 5%, corn 29%, soybean meal 12%, soybean oil 4%, soybean 13%, rice 19%, starch 7%, cellulose 2%, mineral nutrition enhancer 0.5%, Chinese multivitamin 0.5%, choline 0.05%, and CaHPO in balance4·2H2O。
Wherein the Chinese vitamins comprise vitamin A and vitamin B at weight ratio of 1:0.5:1.5:0.8:0.3:1.4:1.11Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3；
The mineral nutrition enhancer comprises the followingThe components by weight percentage are as follows: FeSO4·7H2O 20％、MnSO4·H2O 31％、ZnSO4·7H2O 23％、CuSO4·5H2O26%, KI 4% and the balance of corn flour.
The preparation method of the feed comprises the following steps: the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases.
Example 2
A calcium deficiency type disease mouse model feed comprises the following components in percentage by weight:
6% of casein, 6% of wheat bran, 28.78% of corn, 12% of soybean meal, 4% of soybean oil, 13% of soybean, 19% of rice, 8% of starch, 2% of cellulose, 1% of mineral nutrition enhancer, 0.1% of Chinese multivitamin, 0.06% of choline and the balance of CaHPO4·2H2O。
Wherein the Chinese vitamins comprise vitamin A and vitamin B at weight ratio of 1:0.5:1.5:0.8:0.3:1.4:1.11Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3；
The mineral nutrition enhancer comprises the following components in percentage by weight: FeSO4·7H2O 19.7％、MnSO4·H2O 31.8％、ZnSO4·7H2O 22.5％、CuSO4·5H2O25%, KI 3.8% and the balance of corn flour.
The preparation method of the feed comprises the following steps: the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases.
Example 3
A calcium deficiency type disease mouse model feed comprises the following components in percentage by weight:
6% of casein, 6% of wheat bran, 27% of corn, 12% of soybean meal, 3% of soybean oil, 13% of soybean, 18% of rice, 8% of starch, 1% of cellulose, 0.5% of mineral nutrition enhancer, 0.1% of Chinese multivitamin, 0.1% of choline and the balance of CaHPO4·2H2O。
WhereinThe Chinese multivitamins comprise vitamin A and vitamin B in a weight ratio of 1:0.5:1.5:0.8:0.3:1.4:1.11Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3；
The mineral nutrition enhancer comprises the following components in percentage by weight: FeSO4·7H2O 19.7％、MnSO4·H2O 31.8％、ZnSO4·7H2O 22.5％、CuSO4·5H2O25%, KI 3.8% and the balance of corn flour.
The preparation method of the feed comprises the following steps: the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases.
Detection of
The contents of the respective components in the feed obtained in example 2 were measured, and the results are shown in table 1.
TABLE 1 calcium deficiency type disease mouse model feed ingredient detection
As can be seen from the data in Table 1, the calcium content in the feed designed by the invention is lower than the standard that each kilogram of daily ration of rats and mice in experiments required in the national standard GB14924.3-2010 contains 10g, and the feed is suitable for being applied to the establishment of a bone disease model caused by low calcium of patients in medical experiments and the study of other pathological changes caused by calcium deficiency.
Examples of the experiments
And (3) feeding 9 healthy mice with the feed obtained in the example 2 for 60 days for 2 times a day, wherein each time is 0.5-1.0 g, and then measuring various physiological indexes of the mice, wherein the results are shown in table 2.
TABLE 2 physiological indices of calcium-deficient mice
As can be seen from the data in Table 2, after the feed obtained in example 2 is used for feeding healthy mice for 60 days, the mice all have the calcium deficiency symptom without other discomfort, which indicates that the feed obtained by the invention successfully induces the mice to generate the calcium deficiency, and can be applied to the establishment of a bone disease model caused by low calcium of patients in medical experiments and the research of other pathological changes caused by calcium deficiency.
Claims (7)
1. The model feed for the rats suffering from the calcium deficiency disease is characterized by comprising the following components in percentage by weight:
5 to 6 percent of casein, 5 to 6 percent of wheat bran, 27 to 29 percent of corn, 11 to 12 percent of soybean meal, 3 to 4 percent of soybean oil, 12 to 13 percent of soybean, 18 to 19 percent of rice, 7 to 8 percent of starch, 1 to 2 percent of cellulose, 0.5 to 1 percent of mineral nutrition enhancer, 0.1 to 0.5 percent of Chinese multi-vitamin, 0.05 to 0.1 percent of choline and the balance of CaHPO4·2H2O；
The mineral nutrition enhancer comprises the following components in percentage by weight:
FeSO4·7H2O 19％～20％、MnSO4·H2O 31％～32％、ZnSO4·7H2O 22％～23％、CuSO4·5H225-26% of O, 3-4% of KI and the balance of corn flour.
2. The calcium deficiency type diseased mouse model feed according to claim 1, comprising the following components in weight percent:
6% of casein, 6% of wheat bran, 28.78% of corn, 12% of soybean meal, 4% of soybean oil, 13% of soybean, 19% of rice, 8% of starch, 2% of cellulose, 1% of mineral nutrition enhancer, 0.1% of Chinese multivitamin, 0.06% of choline and the balance of CaHPO4·2H2O。
3. The calcium-deficient diseased mouse model feed according to claim 1 or 2, wherein the corn is corn grade GB 1.
4. The calcium-deficient disease mouse model feed according to claim 1 or 2, wherein the soybeans are soybeans of NY/T2 grade.
5. The calcium deficient diseased mouse model feed according to claim 1 wherein the mineral nutrition fortifier comprises the following components in weight percent:
FeSO4·7H2O 19.7％、MnSO4·H2O 31.8％、ZnSO4·7H2O 22.5％、CuSO4·5H2o25%, KI 3.8%, and the balance being corn flour.
6. The calcium-deficient diseased mouse model feed according to claim 1 or 2, wherein the chinese multivitamins comprise the following components:
vitamin A and vitamin B1Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3(ii) a Wherein, vitamin A and vitamin B1Vitamin B2Vitamin B6Vitamin D3Vitamin C and vitamin K3The weight ratio of (1: 0.5:1.5:0.8:0.3:1.4: 1.1).
7. The method for preparing a model feed for rats with calcium deficiency diseases according to any one of claims 1 to 6, comprising the steps of:
the components are mixed according to the formula and stirred evenly to obtain the mouse model feed for the calcium deficiency type diseases.
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| CN101911923A (en) * | 2010-07-22 | 2010-12-15 | 重庆医科大学附属儿童医院 | Method for constructing preconception-starting vitamin A-deficiency female mouse and newborn mouse animal models |
| CN103704175A (en) * | 2014-01-20 | 2014-04-09 | 四川大学 | Method for building female SD rat iron-deficiency anemia model |
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| CN105685426A (en) * | 2014-11-26 | 2016-06-22 | 广西大学 | Special feed for laboratory mice and preparation method thereof |
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| CN101911923A (en) * | 2010-07-22 | 2010-12-15 | 重庆医科大学附属儿童医院 | Method for constructing preconception-starting vitamin A-deficiency female mouse and newborn mouse animal models |
| CN103704175A (en) * | 2014-01-20 | 2014-04-09 | 四川大学 | Method for building female SD rat iron-deficiency anemia model |
| CN105685426A (en) * | 2014-11-26 | 2016-06-22 | 广西大学 | Special feed for laboratory mice and preparation method thereof |
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