CN104161265A - Preparation method for low-molecular weight soybean peptide-calcium chelate for enhancing bone density - Google Patents
Preparation method for low-molecular weight soybean peptide-calcium chelate for enhancing bone density Download PDFInfo
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- CN104161265A CN104161265A CN201410320336.6A CN201410320336A CN104161265A CN 104161265 A CN104161265 A CN 104161265A CN 201410320336 A CN201410320336 A CN 201410320336A CN 104161265 A CN104161265 A CN 104161265A
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- 244000068988 Glycine max Species 0.000 title claims abstract description 64
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 230000037182 bone density Effects 0.000 title claims abstract description 20
- 230000002708 enhancing effect Effects 0.000 title abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 32
- 108091005804 Peptidases Proteins 0.000 claims abstract description 17
- 239000004365 Protease Substances 0.000 claims abstract description 17
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 17
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 16
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 16
- 238000004108 freeze drying Methods 0.000 claims abstract description 13
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 239000003513 alkali Substances 0.000 claims abstract description 7
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract 3
- 239000000243 solution Substances 0.000 claims description 21
- 239000000047 product Substances 0.000 claims description 7
- 239000012460 protein solution Substances 0.000 claims description 7
- 102000005158 Subtilisins Human genes 0.000 claims description 6
- 108010056079 Subtilisins Proteins 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 102000009027 Albumins Human genes 0.000 claims description 2
- 108010088751 Albumins Proteins 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000011575 calcium Substances 0.000 abstract description 35
- 229910052791 calcium Inorganic materials 0.000 abstract description 35
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 29
- 239000013522 chelant Substances 0.000 abstract description 5
- 229920001184 polypeptide Polymers 0.000 abstract description 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 239000002131 composite material Substances 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000013589 supplement Substances 0.000 abstract description 2
- 230000009849 deactivation Effects 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 210000000689 upper leg Anatomy 0.000 description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 206010006956 Calcium deficiency Diseases 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 238000002389 environmental scanning electron microscopy Methods 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000007849 functional defect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
- A23L33/165—Complexes or chelates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Mycology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention relates to a preparation method for a low-molecular weight soybean peptide-calcium chelate for enhancing bone density, and belongs to the technical field of health-care food or biology. The preparation method comprises the following steps: (1) protease enzymolysis; (2) enzyme deactivation; (3) centrifugation and freeze drying; and (4) the preparation of the soybean peptide-calcium chelate. In the preparation method disclosed by the invention, isolated soybean proteins are used as raw materials, soybean peptide which is obtained through the enzymolysis of the isolated soybean proteins by alkali protease is chelated with calcium chloride, and the soybean peptide-calcium chelate is obtained, so that the utilization rate of calcium is increased. The soybean peptide-calcium chelate has multiple nutrient values of polypeptide and calcium elements, provides superior protein sources, supplements the calcium elements for human bodies, and further promotes the calcium absorption of the human bodies. The peptide which is isolated and purified from the isolated soybean proteins is combined with the calcium, and the preparation method has broad development prospects and considerable economic values. The soybean peptide-calcium composite which is prepared by the method has small molecular weight, the calcium combining weight of the chelate is 78.73mg/g, and the soybean peptide-calcium composite has obvious efficacy of enhancing the bone density.
Description
Technical field
The invention belongs to health food or biological technical field, relate to a kind of preparation method who strengthens bone density effect low-molecular-weight Soybean Peptide-calcium chelate that possesses.
Background technology
Calcium is the fifth-largest element that is only second to C, H, O, N in human body, to human physiological metabolism's important in inhibiting, is the nutritional labeling of needed by human.The aspects such as calcium is the main component that forms bone, tooth, the acid-base balance in blood coagulation and anticoagulation, the telescopicing performance that maintains muscle, adjusting nerve, body all play an important role.The intake of urban and rural residents of China calcium is generally not enough, and calcium deficiency crowd is up to more than 90%.The shortage of calcium can cause all obstacles of human body, as angiocardiopathy, depauperation, rickets, neuroticism, osteoporosis etc.Pregnant woman wet nurse, the elderly, infant, very easily calcium deficiency of especially postclimacteric women, is the key population of replenishing the calcium.
China's soybean yields is abundant, and bean product is with a long history especially, but its utilization rate is lower and have numerous functional defects.In order to improve the functional characteristic of soybean protein, improve soybean protein utilization rate, widen its application, therefore proposed hydrolytic soya bean protein, i.e. the research of soya-bean polypeptides and exploitation.Compared with soybean protein and goods thereof, Soybean Peptide is is more easily digested and assimilated by body, and the material that contains some physiologically active has different physiological roles in body.Along with the fast development of domestic and international proteolysis technology, taking soybean as raw material, to utilize proteolysis technology to produce polypeptide products and be constantly developed, some puts into production.
At present, occurred third generation calcium-supplementing preparation-amino acid chelated calcium on market, but its effect of supplemented calcium is not so good as polypeptide and calcium chelating product, peptide is combined and is more easily absorbed by body than amino acid with calcium.
Summary of the invention
The object of the present invention is to provide a kind of preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate that has, soybean peptide-calcium composite prepared by the method has that molecular weight is little, chelate calcium binding capacity is 78.73mg/g, has obvious enhancing bone density effect.
The object of the invention is to be achieved through the following technical solutions:
Have a preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, taking soybean protein isolate as raw material, adopt the techniques such as Alcalase hydrolysis by novo, enzyme-deactivating, centrifugal, freeze drying, chelating to be prepared from, concrete steps are as follows:
(1) protease hydrolyzed: get soybean protein isolate soluble in water, make protein solution, can heat and accelerate its dissolving, the mass ratio of controlling soybean protein isolate and water is 1: 15～1: 25, after adding the protease that accounts for protein solution quality 3～5%, use the NaOH of 1mol/L that protein solution is adjusted to Alcalase alkali protease optimal pH 8.0, be to carry out enzymolysis under 30～50 DEG C of conditions in temperature, in enzymolysis process, do not stop to add sodium hydroxide solution, all the time the pH value of controlled enzymatic hydrolysis liquid is 8.0 ± 0.01, enzymolysis 1～3h with this understanding.
(2) enzyme that goes out: after protease hydrolyzed reaction finishes, solution is warming up to rapidly 80～100 DEG C, and enzyme 5～15min goes out.
(3) centrifugal, freeze drying: be cooled to room temperature after the enzyme that goes out, pH is transferred to 7.0, centrifugal (4000rpm/min, 20min), collects supernatant, i.e. Soybean Peptide solution, freeze drying, obtains the freeze-drying prods of Soybean Peptide.
(4) preparation of Soybean Peptide-calcium chelate: Soybean Peptide freeze-drying prods is configured to the peptide solution of 40～60g/L and mixes by the mass ratio of 1: 1～3: 1 with 0.02～0.04mol/L calcium chloride, regulating pH value is 6.6, in 25～38 DEG C of water-baths, heat, reaction time is 45～60min, reaction finishes rear solution to be carried out to freeze drying, obtains Soybean Peptide-calcium chelate product.
In the present invention, described soybean separation protein albumin content is more than 90%.
In the present invention, described protease is Alcalase alkali protease, and addition is 5% (g/g).
In the present invention, described in to add Soybean Peptide solution concentration be 50g/L, calcium chloride solution concentration is 50.04mol/L, peptide and calcium mass ratio are 1.6: 1.
The present invention has following beneficial effect:
1, the present invention is taking soybean protein isolate as raw material, and the Soybean Peptide obtaining after alkali protease enzymolysis and calcium chloride chelating, wait until Soybean Peptide-calcium chelate, thereby improves calcium utilization.The multiple battalion that Soybean Peptide-calcium chelate possesses polypeptide and calcium constituent is worth: good protein source be provided and supplement human calcium's element, more having promoted the absorption of human body to calcium.From soybean protein isolate, separation and purification peptide is combined with calcium, has vast potential for future development and considerable economic worth.
2, low-molecular-weight produced according to the invention possesses the Soybean Peptide-calcium chelate that strengthens bone density effect, and its solubility is 98.59% ± 0.17%; It is overall 1.76% that relative molecular weight distribution > 5000Da accounts for, and 5000Da～1000Da is that 18%, < 1000Da is 80.24%; Calcium content is 78.73mg/g; The discovery of ESEM picture, this chelate mouse femur close quarters of feeding is greater than calcium carbonate group and control group, has obvious enhancing bone density effect.
Brief description of the drawings
Fig. 1 is low calcium control group femur Electronic Speculum figure (amplifying 40 times);
Fig. 2 is low calcium control group femur Electronic Speculum figure (amplifying 200 times);
Fig. 3 is calcium carbonate control group femur Electronic Speculum figure (amplifying 40 times);
Fig. 4 is calcium carbonate control group femur Electronic Speculum figure (amplifying 200 times);
Fig. 5 is test group femur Electronic Speculum figure (amplifying 40 times);
Fig. 6 is test group femur Electronic Speculum figure (amplifying 200 times);
Fig. 7 Soybean Peptide-calcium chelate graph of molecular weight distribution;
Fig. 8 is mice serum calcium content figure.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme of the present invention is further described; but do not limit to so; every technical solution of the present invention is modified or is equal to replacement, and not departing from the spirit and scope of technical solution of the present invention, all should be encompassed in protection scope of the present invention.
The manufacture of embodiment 1, low-molecular-weight Soybean Peptide
Taking soybean protein isolate as raw material, soybean protein isolate and water dissolve with the ratio of 1: 20 (g/g), make the protein solution that concentration of substrate is 5% (g/g), Alcalase alkali protease addition (E/S) is 5% (g/g), add afterwards the NaOH of 1mol/L that protein solution is adjusted to Alcalase alkali protease optimal pH 8.0, in 50 DEG C of water-baths, heat, in enzymolysis process, do not stop to add sodium hydroxide solution, all the time the pH value of controlled enzymatic hydrolysis liquid is 8.04 ± 0.01, enzymolysis 2h with this understanding, after question response finishes, solution is warming up to rapidly 90 DEG C, enzyme 10min goes out, be cooled to rapidly afterwards room temperature, regulate pH value to 7.0, centrifugal (4000rpm, 20min), collect supernatant, be Soybean Peptide solution, Soybean Peptide solution is first carried out to pre-freeze, freeze drying afterwards, obtain Soybean Peptide goods.
The preparation of embodiment 2, Soybean Peptide-calcium chelating product
The Soybean Peptide goods of preparing according to embodiment 1, be configured to the peptide solution of 50g/L and mix by the mass ratio of 1.6: 1 with 0.04mol/L calcium chloride, regulating pH value is 6.6, after fully mixing, at 38 DEG C, react 44min, Soybean Peptide solution is fully reacted with calcium chloride, carry out chelating, finally obtain the chelate product of Soybean Peptide-calcium, under this condition, obtain chelate calcium content and reach 78.73mg/g.
Embodiment 3, Soybean Peptide-calcium chelate property testing
Solubility to embodiment 2 gained Soybean Peptide-calcium chelates and relative molecular weight distribute and measure, obtaining its solubility is that 98.59% ± 0.17%, Fig. 7 has reflected Soybean Peptide-calcium chelate molecular weight distribution situation: Soybean Peptide-calcium chelate of molecular weight > 5000Da accounts for overall 1.76%; 5000Da～3000Da accounts for overall 2.79%; 3000Da～2000Da accounts for overall 3.51%; 2000Da～1000Da accounts for overall 11.70%; 1000Da～500Da accounts for overall 25.87%; 500Da～180Da accounts for overall 47.91%; < 180Da accounts for overall 6.64%.Hence one can see that molecular weight < 5000Da accounts for the overwhelming majority, is overall 98.24%, and wherein 500～180Da content is maximum.Because the molecular weight of high quality soybean peptide is less than 1000Da, this kind of Soybean Peptide more easily absorbs, and this experiment gained be less than Soybean Peptide-calcium chelate of 1000Da account for overall 80.24%, therefore Soybean Peptide-calcium chelate that this experiment is produced is more easily absorbed by body.
Embodiment 4, Soybean Peptide-calcium chelate Evaluation of Functional
The present embodiment on the absorptivity of Soybean Peptide-calcium chelate with and long on mice serum calcium, femur, femur is heavy and impact femoral bmd is measured.In Mice Body, 4 weeks feeding experiments draw: the absorptivity of Soybean Peptide-calcium chelate can reach 59.81% ± 3.92%, are significantly higher than calcium carbonate group 53.31% ± 2.9% (P < 0.05); Fig. 8 has reflected mice serum calcium content: the mice serum calcium content of test group and calcium carbonate group is respectively 23.1mg ± 0.08mg and 22.72mg ± 0.62mg is significantly higher than control group 16.9mg ± 2.02mg (P < 0.05), but between test group and calcium carbonate group without significant difference (P > 0.05); Table 1 has reflected that mouse femur is heavy, femur is long: the femur of test group mouse is heavily significantly higher than calcium carbonate group 54.50mg ± 11.02mg and control group 58.25mg ± 7.68mg (P < 0.05) for 66.33mg ± 6.66mg, and femur is grown three groups without significant difference (P > 0.05); Mouse Bone density situation has been reflected in Fig. 1～6, the discovery of ESEM picture, and test group femur close quarters is greater than calcium carbonate group and control group.
Table 1 mouse femur is heavy, femur is long
Note: same row letter identical table differential different not remarkable (P > 0.05), the different significant differences (P < 0.05) that represent of letter.
Claims (9)
1. there is a preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, it is characterized in that described method step is as follows:
(1) protease hydrolyzed: get soybean protein isolate soluble in water, make protein solution, the mass ratio of controlling soybean protein isolate and water is 1: 15～1: 25, after adding the protease that accounts for protein solution quality 3～5%, being adjusted to pH8.0, is to carry out enzymolysis 1～3h under 30～50 DEG C of conditions in temperature;
(2) enzyme that goes out: after protease hydrolyzed reaction finishes, solution is warming up to rapidly 80～100 DEG C, and enzyme 5～15min goes out;
(3) centrifugal, freeze drying: be cooled to room temperature after the enzyme that goes out, pH is transferred to 7.0, centrifugal, collects supernatant, i.e. Soybean Peptide solution, and freeze drying, obtains the freeze-drying prods of Soybean Peptide;
(4) preparation of Soybean Peptide-calcium chelate: Soybean Peptide freeze-drying prods is configured to the peptide solution of 40～60g/L and mixes by the mass ratio of 1: 1～3: 1 with 0.02～0.04mol/L calcium chloride, regulating pH value is 6.6, in 25～38 DEG C of water-baths, heat, reaction time is 45～60min, reaction finishes rear solution to be carried out to freeze drying, obtains Soybean Peptide-calcium chelate product.
2. have according to claim 1 the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, the mass ratio that it is characterized in that described soybean protein isolate and water is 1: 20.
3. according to thering is the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate described in claim 1 or 2, it is characterized in that described soybean separation protein albumin content is more than 90%.
4. there is according to claim 1 the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, it is characterized in that described protease is Alcalase alkali protease.
5. according to having the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate described in claim 1 or 4, the addition that it is characterized in that protease is 5%.
6. there is according to claim 1 the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, it is characterized in that described peptide solution concentration is 50g/L.
7. there is according to claim 1 the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, it is characterized in that described calcium chloride solution concentration is 50.04mol/L.
8. according to thering is the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate described in claim 1,6 or 7, it is characterized in that described peptide solution and calcium chloride mass ratio are 1.6: 1.
9. have according to claim 1 the preparation method who strengthens bone density low-molecular-weight Soybean Peptide-calcium chelate, it is characterized in that described centrifugation rotating speed is 4000rpm/min, the centrifugation time is 20min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707879A (en) * | 2016-01-28 | 2016-06-29 | 安徽中森生物技术有限公司 | Preparation method of soybean peptide biological calcium |
| CN106244655A (en) * | 2016-08-05 | 2016-12-21 | 广州金酮医疗科技有限公司 | A kind of protein peptide and calcium chelate thereof and preparation method |
| CN108201137A (en) * | 2018-01-22 | 2018-06-26 | 武汉维奥制药有限公司 | A kind of preparation method of amino-acid chelate |
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| JPS57186455A (en) * | 1981-05-07 | 1982-11-16 | Higeta Shoyu Kk | Enzymatic digestion of undenatured soybean protein |
| CN101161114A (en) * | 2007-11-02 | 2008-04-16 | 中国农业大学 | Method of producing soybean peptide-calcium compound |
| CN103040000A (en) * | 2011-10-11 | 2013-04-17 | 财团法人食品工业发展研究所 | Preparation method of soybean peptide-calcium chelate |
-
2014
- 2014-07-04 CN CN201410320336.6A patent/CN104161265A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57186455A (en) * | 1981-05-07 | 1982-11-16 | Higeta Shoyu Kk | Enzymatic digestion of undenatured soybean protein |
| CN101161114A (en) * | 2007-11-02 | 2008-04-16 | 中国农业大学 | Method of producing soybean peptide-calcium compound |
| CN103040000A (en) * | 2011-10-11 | 2013-04-17 | 财团法人食品工业发展研究所 | Preparation method of soybean peptide-calcium chelate |
Non-Patent Citations (2)
| Title |
|---|
| 孙莉洁等: "响应面法优化大豆肽与钙离子螯合的研究", 《中国粮油学报》 * |
| 张美玲等: "大豆蛋白水解物的酶法修饰及其亚铁和钙离子的螯合能力", 《食品与发酵工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707879A (en) * | 2016-01-28 | 2016-06-29 | 安徽中森生物技术有限公司 | Preparation method of soybean peptide biological calcium |
| CN105707879B (en) * | 2016-01-28 | 2019-04-19 | 安徽中森生物技术有限公司 | A kind of preparation method of soybean peptide BIOLOGICAL CALCIUM |
| CN106244655A (en) * | 2016-08-05 | 2016-12-21 | 广州金酮医疗科技有限公司 | A kind of protein peptide and calcium chelate thereof and preparation method |
| CN108201137A (en) * | 2018-01-22 | 2018-06-26 | 武汉维奥制药有限公司 | A kind of preparation method of amino-acid chelate |
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Application publication date: 20141126 |