CN101161114A - Method of producing soybean peptide-calcium compound - Google Patents
Method of producing soybean peptide-calcium compound Download PDFInfo
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- CN101161114A CN101161114A CNA2007101767561A CN200710176756A CN101161114A CN 101161114 A CN101161114 A CN 101161114A CN A2007101767561 A CNA2007101767561 A CN A2007101767561A CN 200710176756 A CN200710176756 A CN 200710176756A CN 101161114 A CN101161114 A CN 101161114A
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Abstract
The present invention discloses a method for preparing soja titanium-calcium complex, belonging to health food or food additive preparing technology field, especially relates to soja deep processing technology. The present invention uses low-temperature degrease bean or soja separating albumen as raw material, by means of technics such as enzymatic hydrolysising of the protease and deamidase, destroying enzyme, chelating soja titanium and calcium, drying milling etc., provides a soja titanium-calcium complex with high calcium combination amount. The soja titanium-calcium complex is canary powder, property is stable, calcium combination amount is 110mg/g. The soja titanium-calcium complex has low cost, simple technics, and can accelerate calcium absorbing, also can be used as calcium-filling product directly or as substrate to add into healty food or general food, its application is wide.
Description
Technical field
The invention belongs to health food or food additives preparing technical field, particularly the preparation method of a kind of soybean peptide-calcium composite of soybean deep processing technology.
Background technology
Calcium is the nutrient of needed by human, for guaranteeing health and building up health, replenishes the calcium scientifically and rationally by COF, and promotes that human body is crucial to the absorption of calcium.The product of replenishing the calcium in the market has inorganic calcium, calcium of organic acid, amino acid chelated calcium, CPP calcium etc.The calcium absorptivity less than 40% of inorganic calcium, calcium of organic acid, amino acid chelated calcium and the CPP calcium absorptivity height that derives from milk, but production cost is also high, has limited its application greatly.Soybean contains rich in protein, discover that the hydroxy-acid group that contains a large amount of glutamic acid and aspartic acid (content＞20%) in the soybean protein can combine with calcium, make the calcium ion of absorption in small intestine, be in soluble status or carry that calcium ion directly enters small intestine cells and the absorption that promotes calcium.
In present research, Japan once adopted the method for ion-exchange to remove phytic acid, obtain combining with calcium and promoting the soybean protein isolate of calcium absorption, but this method can increase production cost, and the adding of calcium simultaneously also can cause soybean protein to form the insolubility precipitation.The Soybean Peptide that domestic correlative study obtains is deacylated tRNA amine not, compares calcium content with soybean peptide-calcium composite of the present invention and obviously reduce.The present invention utilizes the external enzymolysis soybean protein isolate of exogenous enzymes to obtain low-molecular-weight deacylated tRNA amine Soybean Peptide, have the characteristics fast that absorb with comparing with protein with the amino acid of forming, behind enzymolysis, add excessive calcium simultaneously and directly remove phytic acid, compare with the method for ion-exchange and to greatly reduce production cost, the soybean peptide-calcium composite that obtains has that dissolubility is good, molecular weight is low, calcium binding capacity height, the characteristics that easily absorb.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of soybean peptide-calcium composite, it is characterized in that, with defatted soybean meal or soybean protein isolate is raw material, adopts protease deamidase enzymolysis, enzyme-deactivating, Soybean Peptide and calcium chelating, dry powder process and obtains the soybean peptide-calcium composite.This method is that a kind of production cost is low, and the low and calcium binding capacity height of phytic acid content is stablized the preparation method of the soluble soybean peptide-calcium composite that bioavailability is high to soda acid processing, heat treatment.
The step of preparation process of described soybean peptide-calcium composite is as follows:
1) protease hydrolyzed: soybean protein being dispersed in the abundant 10～15min of stirring in the water, pH is transferred to 2 ~ 4, is under the 45-55 ℃ of condition in temperature, add protease insulation hydrolysis 40-60min, 4, centrifugal 20min under the 000rpm rotating speed collects supernatant and obtains Soybean Peptide solution;
2) deacylated tRNA amine: the Soybean Peptide pH value of solution is transferred to 6～8, is under the 45-55 ℃ of condition in temperature, adds deamidase and carries out deacylated tRNA amine 120-180min, obtains the Soybean Peptide solution of deacylated tRNA amine;
3) modulation soluble soybean peptide-calcium composite:, under 25～45 ℃ of temperature, add ionic calcium soln with the Soybean Peptide solution of deacylated tRNA amine, behind the sufficient reacting, 6, centrifugal 15min under the 000rpm rotating speed, collect supernatant, dry powder process obtains the soluble soybean peptide-calcium composite.
Described soybean protein is defatted soybean meal modulation or uses soybean protein isolate with commerce that protein content is more than 85%.
Described soybean protein concentration is 1%～5%.(w/v)
Described protease is protease M (protease M, amano pharmaceutical), and addition is the 0.5wt%～1.5wt% of substrate.
Described deamidase is glutaminase or asparaginase or their mixture.(glutaminase, amano pharmaceutical), addition are the 1wt%～3wt% of substrate.
CaCl in the described adding deacylated tRNA amine Soybean Peptide solution
2Solution concentration is 1mol/L, and ratio is 30: 1 (v/v), and reaction temperature is that 25～37 ℃, reaction time are 10～40min, centrifugal (4,000rpm 20min), gets the supernatant drying, the soybean peptide-calcium composite.
The invention has the beneficial effects as follows the soluble soybean peptide calcium complexes of producing according to above soluble soybean peptide-calcium composite manufacture method, is that three components of 24000Da, 14400Da and 9330Da are formed by mean molecule quantity; Peptide content (with protein refractometer) reaches 80%; The calcium binding capacity reaches more than the 110mg/g; Phytic acid content is less than 0.55%; Stable in properties in pH 3-10; Solid-state is pale yellow powder; Soluble in water.Can be directly as the high efficiency calcium-enriching product, or make an addition to health products or bread and cheese as base-material.
The soluble soybean peptide-calcium composite production cost that this method is produced is low, technology is simple, bioavailability is high.This method is that new direction has been opened up in soybean protein deep processing field.
The specific embodiment
The invention provides a kind of preparation method of soybean peptide-calcium composite.This method is a raw material with defatted soybean meal or soybean protein isolate, adopts protease deamidase enzymolysis, enzyme-deactivating, Soybean Peptide and calcium chelating, dry powder process and obtains the soybean peptide-calcium composite.This method is that a kind of production cost is low, and the low and calcium binding capacity height of phytic acid content is stablized the preparation method of the soluble soybean peptide-calcium composite that bioavailability is high to soda acid processing, heat treatment.Enumerating embodiment is below further specified the present invention.
Embodiment 1, with the defatted soybean meal method that raw material is produced the soluble soybean peptide-calcium composite
Defatted soybean meal and water are to extract 90min under 7.0～8.0 conditions with the ratio of 1: 10 (w/v) at pH, centrifugal (4,000rpm, 20min) remove bean dregs and obtain protein curd, add dispersed with stirring behind 5 times of volume water, centrifugal (1,000rpm 5min), discards the whey (repeating 2 times) on upper strata; To obtain protein curd and add water in 5% (m/v) ratio, the powerful stirring evenly disperseed curdled milk, transfer pH to 3.0,50 ℃ of insulations, add protease M (the enzyme-to-substrate ratio is 1/100) hydrolysis 60min, hydrolysis is heated to 85 ℃ after finishing, the 10min enzyme that goes out, cooling, it is centrifugal that (4000rpm 20min), collects supernatant, the Soybean Peptide pH value of solution is transferred to 7.0, temperature adds glutaminase (the enzyme-to-substrate ratio is 1/50) and carries out deacylated tRNA amine 180min under 50 ℃ of conditions, and enzymolysis is heated to 85 ℃ after finishing, the 10min enzyme that goes out obtains the Soybean Peptide solution of deacylated tRNA amine.Ratio in 30: 1 (v/v) adds 1mol/L CaCl
2Solution, reaction is 30 minutes under 37 ℃ of conditions, centrifugal (4,000rpm, 20min), get supernatant, freeze drying promptly gets the soybean peptide-calcium composite.
Embodiment 2, with the defatted soybean meal method that raw material is produced the soluble soybean peptide-calcium composite
Defatted soybean meal and water are to extract 90min under 7.0～8.0 conditions with the ratio of 1: 10 (w/v) at pH, centrifugal (4,000rpm, 20min) remove bean dregs and obtain protein curd, add dispersed with stirring behind 5 times of volume water, centrifugal (1,000rpm, 5min), discard the whey (repeating 2 times) on upper strata, obtain protein curd and add water in 5% (m/v) ratio, be stirred to even dispersion liquid with homogenizer, pH transfers to 3.0,45 ℃ of insulations, adds protease M (the enzyme-to-substrate ratio is 1/100) at 45 ℃ of insulation hydrolysis 60min, hydrolysis is heated to 85 ℃ after finishing, the 10min enzyme that goes out, cooling, centrifugal (4000rpm, 20min), collect supernatant, the Soybean Peptide pH value of solution is transferred to 7.0, temperature is under 55 ℃ of conditions, add asparaginase (the enzyme-to-substrate ratio is 1/50) and carry out deacylated tRNA amine 180min, be heated to 85 ℃ after enzymolysis finishes, the 10min enzyme that goes out obtains the Soybean Peptide solution of deacylated tRNA amine.Ratio in 30: 1 (v/v) adds 1mol/L CaCl
2Solution, reaction is 30 minutes under 25 ℃ of conditions, centrifugal (4,000rpm, 20min), get supernatant, freeze drying promptly gets the soybean peptide-calcium composite.
Embodiment 3, be the method that raw material is produced the soluble soybean peptide-calcium composite with commercial soybean protein isolate
Soybean protein isolate is added 10 times of volume water be stirred to even dispersion liquid with homogenizer, pH transfers to 3.0,55 ℃ of insulations, add protease M (the enzyme-to-substrate ratio is 1/100), 55 ℃ of insulation hydrolysis 60min, after finishing, hydrolysis is heated to 85 ℃, the 10min enzyme that goes out, cooling, centrifugal (4,000rpm 20min), collects supernatant, the Soybean Peptide pH value of solution is transferred to 7.0, temperature adds glutaminase (the enzyme-to-substrate ratio is 1/50) and carries out deacylated tRNA amine 180min under 50 ℃ of conditions, obtains the Soybean Peptide solution of deacylated tRNA amine.Ratio in 30: 1 (v/v) adds 1mol/L CaCl
2Solution, reaction is 30 minutes under 30 ℃ of conditions, centrifugal (4,000rpm, 20min), get supernatant, freeze drying promptly gets the soybean peptide-calcium composite.
Embodiment 4, be the method that raw material is produced the soluble soybean peptide-calcium composite with commercial soybean protein isolate
Soybean protein isolate is added 10 times of volume water be stirred to even dispersion liquid with homogenizer, pH transfers to 3.0,45 ℃ of insulations, add protease M (the enzyme-to-substrate ratio is 1/100), 45 ℃ of insulation hydrolysis 60min, after finishing, hydrolysis is heated to 85 ℃, the 10min enzyme that goes out, cooling, centrifugal (4,000rpm 20min), collects supernatant, the Soybean Peptide pH value of solution is transferred to 7.0, temperature adds asparaginase (the enzyme-to-substrate ratio is 1/50) and carries out deacylated tRNA amine 180min under 45 ℃ of conditions, obtains the Soybean Peptide solution of deacylated tRNA amine.Ratio in 30: 1 (v/v) adds 1mol/L CaCl
2Solution, reaction is 30 minutes under 28 ℃ of conditions, centrifugal (4,000rpm, 20min), get supernatant, freeze drying promptly gets the soybean peptide-calcium composite.
Embodiment 5, be the method that raw material is produced the soluble soybean peptide-calcium composite with commercial soybean protein isolate
Soybean protein isolate is added 10 times of volume water be stirred to even dispersion liquid with homogenizer, pH transfers to 3.0,55 ℃ of insulations, add protease M (the enzyme-to-substrate ratio is 1/100), 55 ℃ of insulation hydrolysis 60min, after finishing, hydrolysis is heated to 85 ℃, the 10min enzyme that goes out, cooling, centrifugal (4,000rpm 20min), collects supernatant, the Soybean Peptide pH value of solution is transferred to 7.0, temperature adds glutaminase and asparaginase (1: 1) (the enzyme-to-substrate ratio is 1/50) and carries out deacylated tRNA amine 180min under 45 ℃ of conditions, obtains the Soybean Peptide solution of deacylated tRNA amine.Add 1mol/L CaCl in 30: 1 ratios
2Solution, reaction is 30 minutes under 25 ℃ of conditions, centrifugal (4,000rpm, 20min), get supernatant, freeze drying promptly gets the soybean peptide-calcium composite.
Claims (9)
1. the preparation method of a soybean peptide-calcium composite is characterized in that, is raw material with defatted soybean meal or soybean protein isolate, adopts protease deamidase enzymolysis, enzyme-deactivating, Soybean Peptide and calcium chelating, dry powder process and obtains the soybean peptide-calcium composite.
2. according to the preparation method of the described soybean peptide-calcium composite of claim 1, it is characterized in that the production technology of described preparation soybean peptide-calcium composite may further comprise the steps:
(1) defatted soybean meal and water were dissolved 60 minutes in 1: 10 ratio, pH 7.0-8.0, centrifugal gets supernatant and transfers pH 4.5 acid heavy, use aqueous dispersion after removing supernatant, the step pH 3.0-4.0 that goes forward side by side makes its dissolving, separate, make soybean protein, its purity is at 85%-90%;
2) protease hydrolyzed: soybean protein being dispersed in the abundant 10～15min of stirring in the water, pH is transferred to 2～4, is under the 45-55 ℃ of condition in temperature, add protease insulation hydrolysis 40-60min, 4, centrifugal 20min under the 000rpm rotating speed collects supernatant and obtains Soybean Peptide solution;
3) deacylated tRNA amine: the Soybean Peptide pH value of solution is transferred to 6～8, is under the 45-55 ℃ of condition in temperature, adds deamidase and carries out deacylated tRNA amine 120-180min, obtains the Soybean Peptide solution of deacylated tRNA amine;
4) modulation soluble soybean peptide-calcium composite:, under 25～45 ℃ of temperature, add ionic calcium soln with the Soybean Peptide solution of deacylated tRNA amine, behind the sufficient reacting, centrifugal 15min under the 6000rpm rotating speed collects supernatant, and dry powder process obtains the soluble soybean peptide-calcium composite.
3. require the preparation method of described soybean peptide-calcium composite according to right 2, it is characterized in that, described protease is protease M (protease M can get from amano pharmaceutical), and addition is the 0.5wt%～1.5wt% of substrate.
4. require the preparation method of described soybean peptide-calcium composite according to right 2, it is characterized in that: described deamidase is glutaminase or asparaginase or their mixture (glutaminase, can get from amano pharmaceutical), addition is the 1wt%～3wt% of substrate.
5. require the preparation method of described soybean peptide-calcium composite according to right 2, it is characterized in that: the enzymolysis of above-mentioned steps (2) is to be 2-5% in the w/v of soy bean proteinous soln concentration, and the weight that adds protease during enzymolysis is the 1-3% of soybean protein weight in the solution.
6. require the preparation method of described soybean peptide-calcium composite according to right 2; It is characterized in that: the enzymolysis of above-mentioned steps (3) is to be 2-5% in the w/v of soy bean proteinous soln concentration, and during enzymolysis, the weight that adds deamidase is the 0.5-2% of soybean protein weight in the solution.
7. according to the preparation method of any described soybean peptide-calcium composite of claim 1-6, it is characterized in that: the degree of hydrolysis of the Soybean Peptide that obtains is: 20-30%.
8. require the preparation method of described soybean peptide-calcium composite according to right 1, it is characterized in that: above-mentioned steps (4) adds ionic calcium soln in the Soybean Peptide solution of deacylated tRNA amine, is to add 1mol/L CaCl with 30: 1 ratio of volume ratio
2Solution reacted 10～40min minute under 25-37 ℃ of condition, and centrifugal (4,000rpm 20min), gets the supernatant drying, gets the soybean peptide-calcium composite.
9. require the preparation method of described soybean peptide-calcium composite according to right 1-7, it is characterized in that, the phytic acid content of resulting soybean peptide-calcium composite is less than 0.55%, and the calcium binding capacity is more than the 110mg/g.
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| CN103040000A (en) * | 2011-10-11 | 2013-04-17 | 财团法人食品工业发展研究所 | Preparation method of soybean peptide-calcium chelate |
| CN103880920A (en) * | 2014-03-06 | 2014-06-25 | 福州大学 | Preparation method of metal chelating peptide |
| CN104161265A (en) * | 2014-07-04 | 2014-11-26 | 东北农业大学 | Preparation method for low-molecular weight soybean peptide-calcium chelate for enhancing bone density |
| CN105707879A (en) * | 2016-01-28 | 2016-06-29 | 安徽中森生物技术有限公司 | Preparation method of soybean peptide biological calcium |
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| CN106244655A (en) * | 2016-08-05 | 2016-12-21 | 广州金酮医疗科技有限公司 | A kind of protein peptide and calcium chelate thereof and preparation method |
| CN106387920A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Preparation method of soybean polypeptide chelated calcium |
| WO2018191449A1 (en) * | 2017-04-13 | 2018-10-18 | Mgpi Processing, Inc. | L-cysteine-treated proteins with altered functionalities and preparation thereof |
| CN111955701A (en) * | 2020-09-03 | 2020-11-20 | 南宁市绿宝食品有限公司 | Sericin-containing composite soybean peptide chelated calcium crispy and fresh pickled vegetables and making method thereof |
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- 2007-11-02 CN CN2007101767561A patent/CN101161114B/en not_active Expired - Fee Related
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| CN103040000A (en) * | 2011-10-11 | 2013-04-17 | 财团法人食品工业发展研究所 | Preparation method of soybean peptide-calcium chelate |
| CN103880920A (en) * | 2014-03-06 | 2014-06-25 | 福州大学 | Preparation method of metal chelating peptide |
| CN103880920B (en) * | 2014-03-06 | 2015-11-18 | 福州大学 | A kind of preparation method of metal chelating peptide |
| CN104161265A (en) * | 2014-07-04 | 2014-11-26 | 东北农业大学 | Preparation method for low-molecular weight soybean peptide-calcium chelate for enhancing bone density |
| CN105707879B (en) * | 2016-01-28 | 2019-04-19 | 安徽中森生物技术有限公司 | A kind of preparation method of soybean peptide BIOLOGICAL CALCIUM |
| CN105707879A (en) * | 2016-01-28 | 2016-06-29 | 安徽中森生物技术有限公司 | Preparation method of soybean peptide biological calcium |
| CN105768086A (en) * | 2016-03-30 | 2016-07-20 | 福建农林大学 | Edible fungus protein peptide-selenium chelate preparation method |
| CN105919094A (en) * | 2016-05-11 | 2016-09-07 | 安徽中森生物技术有限公司 | Immunity improving and fatigue preventing composition and preparation method thereof |
| CN106244655A (en) * | 2016-08-05 | 2016-12-21 | 广州金酮医疗科技有限公司 | A kind of protein peptide and calcium chelate thereof and preparation method |
| CN106387920A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Preparation method of soybean polypeptide chelated calcium |
| WO2018191449A1 (en) * | 2017-04-13 | 2018-10-18 | Mgpi Processing, Inc. | L-cysteine-treated proteins with altered functionalities and preparation thereof |
| US11968998B2 (en) | 2017-04-13 | 2024-04-30 | Mgpi Processing, Inc. | L-cysteine-treated proteins with altered functionalities and preparations thereof |
| CN111955701A (en) * | 2020-09-03 | 2020-11-20 | 南宁市绿宝食品有限公司 | Sericin-containing composite soybean peptide chelated calcium crispy and fresh pickled vegetables and making method thereof |
| CN116473242A (en) * | 2023-06-17 | 2023-07-25 | 河南科拓生物科技有限公司 | Preparation method and application of food additive rich in soybean polypeptide |
| CN118383527A (en) * | 2024-06-25 | 2024-07-26 | 海阳市三丰生化有限公司 | Cartilage powder containing secondary collagen extracted from chicken cartilage and its preparation method |
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