CN105768086A - Edible fungus protein peptide-selenium chelate preparation method - Google Patents
Edible fungus protein peptide-selenium chelate preparation method Download PDFInfo
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- 239000011669 selenium Substances 0.000 title claims abstract description 75
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 70
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 66
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- 238000002360 preparation method Methods 0.000 title claims abstract description 22
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- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 10
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- 240000000599 Lentinula edodes Species 0.000 claims description 4
- 235000007685 Pleurotus columbinus Nutrition 0.000 claims description 4
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- 241001506047 Tremella Species 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
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- 241000123113 Phellinus igniarius Species 0.000 claims description 3
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- 238000012870 ammonium sulfate precipitation Methods 0.000 claims 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 26
- 150000001413 amino acids Chemical class 0.000 abstract description 6
- 102000035195 Peptidases Human genes 0.000 abstract description 2
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- 235000018102 proteins Nutrition 0.000 description 39
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 24
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- HSTOKWSFWGCZMH-UHFFFAOYSA-N 3,3'-diaminobenzidine Chemical compound C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 HSTOKWSFWGCZMH-UHFFFAOYSA-N 0.000 description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 2
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- PMYDPQQPEAYXKD-UHFFFAOYSA-N 3-hydroxy-n-naphthalen-2-ylnaphthalene-2-carboxamide Chemical compound C1=CC=CC2=CC(NC(=O)C3=CC4=CC=CC=C4C=C3O)=CC=C21 PMYDPQQPEAYXKD-UHFFFAOYSA-N 0.000 description 1
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- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention provides an edible fungus protein peptide-selenium chelate preparation method. The preparation method comprises the following steps: an alkali-extraction and acid-precipitation method or an ammonium sulfate precipitation method is used to extract proteins in edible fungi; alkaline proteases, neutral proteases or compound proteases are used to conduct restriction enzyme digestion for the edible fungi, the enzymes are inactivated, and an edible fungus protein enzymatic hydrolysate is prepared; and selenium in inorganic sodium selenite and edible fungus protein peptides are chelated to obtain the edible fungus peptide-selenium chelate. The alkali-extraction and acid-precipitation method or the ammonium sulfate precipitation method can significantly increase the extraction rate of the edible fungus proteins. By controlling the enzyme digestion time, the degradation degree is effectively controlled. The protein peptide-selenium chelate preparation technology is simple. The prepared fungus protein peptide-selenium chelate has a unique chelation structure and a transport mechanism, is easily absorbed, safe, non-toxic and low in prices, at the same time can supplement amino acids and selenium, and becomes a first choice of a selenium supplement. The preparation method provides a new way for the application of the edible fungi.
Description
Technical field
The present invention relates to the preparation method of a kind of edible fungus protein peptide-selenium chelate, belong to technical field of food biotechnology.
Background technology
Selenium (Se) is the important mineral element of one of needed by human, has important physiological function, and existing numerous studies show that a variety of disease is all closely related with selemium nutrition situation.Human body mainly obtains selenium by diet, and the method for artificial selenium supplement is oral containing selenium preparation.The method inorganic selenium (sodium selenite and sodium selenate) of general selenium supplement makes oral formulations.But, inorganic selenium hardening agent absorbs and the effect of utilization is undesirable, and biological effectiveness is low, and has stronger toxic and side effects, and minimum lethal dose is relatively small, and between toxic dose and requirement, scope is little, there is bigger potential safety hazard, thus is strictly limited it and uses.Research display, inorganic selenium organic selenium compounds toxicity after bioconversion is low, and absorbance is higher, and bioavailability and biological activity are the highest, more notable than inorganic selenium on challenge.
Edible and medical fungi is the higher fungus that a big class has large-scale sporophore, it is commonly called as mushroom or gill fungus, the multiple nutritional components wanted containing needed by human body, protein content is the highest, protein content such as Flammulina velutiper (Fr.) Sing reaches 20.9%, in Volvariella volvacea (Bull.Ex Franch.) Singer．, protein content reaches 25.9%, substantially exceeds vegetable and grain and food that we often eat, also above the protein content of poultry product.Edible and medical fungi is rich in each amino acid, such as total amino acid content average out to 15.76%(dry weights such as Lentinus Edodes, Pleurotus ostreatus, Tremella), it is necessary to total amino acid content average out to 6.43%(dry weight), its delicious flavour is relevant with nucleotide containing a large amount of free amino acids with in mushroom body.Therefore, the polypeptide that the Proteolytic enzyme in edible and medical fungi produces has more rich biological activity and higher nutritive value compared with common animal and vegetable protein.
Utilize biotechnology to edible and medical fungi (Lentinus Edodes, Auricularia, Pleurotus ostreatus, crab flavour mushroom, agrocyb eaegerita, Poria, Phellinus igniarius (L. ex Fr.) Quel., Volvariella volvacea (Bull.Ex Franch.) Singer．, Flammulina velutiper (Fr.) Sing, Pleurotus eryngii, Tremella, Caulis Bambusae In Taeniam etc.) carry out the deep processing of protein resource, carry out the screening of biologically active peptide and extracting and separate, then utilize inorganic selenium to chelate, prepare polypeptide-selenium chelate, inorganic selenium are transformed into organic selenium.This edible and medical fungi biologically active polypeptide-selenium chelate can significantly improve absorbance and the utilization rate of selenium element, but also there is antioxidation, antibacterial, immunomodulating, blood fat reducing and blood sugar lowering isoreactivity, there are the highest Development volue and application prospect, the added value of edible and medical fungi can be improved simultaneously, utilization for edible and medical fungi resource opens new approach, has wide market prospect.
Summary of the invention
Present invention aims to the defect that prior art exists, it is provided that the preparation method of a kind of edible fungus protein peptide-selenium chelate, there is the advantage that technological operation is easy, safety is high.
The preparation method of the edible fungus protein peptide-selenium chelate of the present invention, comprises the steps:
(1) alkali extraction-acid precipitation or ammonium sulfate precipitation method is utilized to extract the protein in edible fungi；
(2) use protease that edible fungus protein is carried out enzymolysis, enzyme denaturing, prepare edible fungus protein polypeptide complex solution；
(3) use solid high-temperature mixing method or the legal selenium made in inorganic matter sodium selenite of chelate liquid to chelate with edible fungus protein peptide, prepare edible fungus protein peptide-selenium chelate；
(4) edible fungus protein peptide-selenium chelate is carried out lyophilization.
Concrete grammar is as follows:
In step (1), by edible fungi micronizing, edible fungi after pulverizing mixes with the ratio of w/v 1:20-60 with distilled water, 0.5-2wt.% NaOH is utilized to regulate pH to 9.0-11.0, supersound extraction 10-50min, 60-120min is extracted in water-bath, temperature 50-100 DEG C, by filtered through gauze after extraction, filtrate is the edible fungus protein solution that alkali carries, and the edible fungus protein pH value of solution carried with hydrochloric acid regulation alkali carries out albumen precipitation to 2.5-4.0, is centrifuged to obtain protein precipitation, it is washed to neutral recentrifuge, pellet frozen is drying to obtain edible fungus protein；Or by edible fungi micronizing, edible fungi after pulverizing mixes with the ratio of 1:20-60w/v with distilled water, being gradually added solid ammonium sulfate makes the saturation of ammonium sulfate reach 40-60%, it is centrifuged and removes supernatant, obtain edible fungus protein precipitation, utilizing dialysis desalination postlyophilization to obtain edible fungus protein, the centrifugal condition of described protein precipitation by centrifugation is 3000-5000r/min, centrifugal 5-10min.
In step (2), edible fungus protein is dissolved in water and makes albumen quality concentration reach 1.0-5.0%, NaOH or KOH solution with 0.5-2wt.% are by pH regulator to 7.0-11.0, temperature is maintained at 40-60 DEG C, add 1.0-5.0wt.% protease, hydrolysis 30-90min, enzyme denaturing, obtain edible fungus protein polypeptide complex solution.
Enzyme described in step (2) uses alkaline protease, neutral protease or compound protease.
In step (3), the edible fungus protein polypeptide complex solution lyophilization that step (2) is obtained, then sodium selenite powder and edible fungus protein peptide freeze-dried powder being mixed according to mass ratio 1:6-5:6, high temperature 100-150 DEG C adds hot preparation edible fungus protein peptide-selenium chelate；Or edible fungus protein polypeptide complex solution step (2) obtained mixes with 0.5-2mol/L sodium selenite solution, both are 6:1-6:5 by volume, stir, regulate pH3.0-10.0, sustained response 1.0-2.0h under the conditions of 30-80 DEG C, cool down after reaction, the removal of impurity is gone in centrifugation, adds the ethanol of 3-5 times of volume 75-95%, and precipitation stands 12-24h, centrifugal segregation supernatant, it is thus achieved that edible fungus protein peptide-selenium chelate.
In step (4), after chelatropic reaction terminates, edible fungus protein peptide-selenium chelate lyophilization.
Described edible fungi includes: Lentinus Edodes, Auricularia, Pleurotus ostreatus, crab flavour mushroom, agrocyb eaegerita, Poria, Phellinus igniarius (L. ex Fr.) Quel., Volvariella volvacea (Bull.Ex Franch.) Singer．, Flammulina velutiper (Fr.) Sing, Pleurotus eryngii, Tremella, one in the edible and medical fungi such as Caulis Bambusae In Taeniam.
The present invention compared with prior art, has the advantage that alkali extraction-acid precipitation or ammonium sulfate precipitation method extract the protein extracting ratio in edible fungi high, the most less document report extracted about edible fungus protein；By controlling edible fungus protein enzymolysis time, obtain the biologically active peptide with high selenium sequestering activity；By chelating with the selenium in inorganic matter sodium selenite, prepare organic biologically active peptide-selenium chelate and there is chelating system and the transporting mechanism of uniqueness, easily absorbed, safety non-toxic, price are low, can supplement aminoacid and plasma selenium simultaneously.The present invention is that the application of edible fungi edible fungi provides new approaches, provides theoretical foundation and technical support for exploitation organic selenium supplementary with functional food.
Detailed description of the invention
Embodiment 1
The first step: by Volvariella volvacea (Bull.Ex Franch.) Singer． micronizing, Volvariella volvacea (Bull.Ex Franch.) Singer． after pulverizing mixes with the ratio of w/v 1:20 with distilled water, 2% NaOH is utilized to regulate PH to 9.0, supersound extraction 10min, 60min is extracted in water-bath, temperature 60 C, by filtered through gauze after extraction, filtrate is the Volvariella volvacea (Bull.Ex Franch.) Singer． protein solution that alkali carries, the Volvariella volvacea (Bull.Ex Franch.) Singer． protein solution pH to 3.0 carried with hydrochloric acid regulation alkali carries out albumen precipitation, and 4000r/min is centrifuged 5min and obtains protein precipitation, is washed to neutral recentrifuge, pellet frozen is drying to obtain Volvariella volvacea (Bull.Ex Franch.) Singer． albumen, its extraction rate reached 71.25%.
Second step: make albumen quality concentration reach 3.0% in water Volvariella volvacea (Bull.Ex Franch.) Singer． protein dissolution, by NaOH solution by Volvariella volvacea (Bull.Ex Franch.) Singer． protein solution pH regulator to 9.0, temperature is maintained at 50 DEG C, adds 3.0% protease, hydrolyzes 90min, enzyme denaturing, obtains Volvariella volvacea (Bull.Ex Franch.) Singer． protein polypeptide complex solution.
3rd step: Volvariella volvacea (Bull.Ex Franch.) Singer． protein polypeptide complex solution is added 0.5mol/L sodium selenite solution for 6:1 ratio by volume, stir, regulation pH7.0, sustained response 1.0h under the conditions of 30 DEG C, cools down after reaction, and the removal of impurity is gone in centrifugation, add the ethanol of 4 times of volumes 75%, precipitation stands 12h, centrifugal segregation supernatant, it is thus achieved that Volvariella volvacea (Bull.Ex Franch.) Singer． protein peptide-selenium chelate.
4th step: after chelatropic reaction terminates, edible fungus protein peptide-selenium chelate lyophilization.
Use 3,3'-diaminobenzidine colorimetric method for determining Se content.
Weigh Volvariella volvacea (Bull.Ex Franch.) Singer． protein peptide-selenium chelate about 0.1g, put in 100mL beaker, add 5mL Digestive system, electric furnace digests being water white transparency to sample liquid.After cooling, with the digestion sample liquid of residual in the distilled water wash flask of about 10mL, it is merged in beaker, above-mentioned Digestive system, regulates about pH7.0 with 40%NaOH and 5%NaOH solution, finally by fixed for solution molten to 50mL, to be measured.Bioassay standard curve: take the separatory funnel of 6 125mL, one accurate addition above-mentioned solution 0. 5mL, another 5 be separately added into 2. 0, the selenium standard solution 5 μ g/mL of 4. 0,6. 0,8. 0,10. 0mL, respectively add distilled water to 40mL, with the regulation of 1mol HCl solution to pH2～3, add 0.
The EDTA-2Na solution 2mL of 2M, is subsequently adding 3.3 '-diaminobenzidine solution (DBA) 2mL of 0. 5%, in the environment of being placed in 60 DEG C of water-bath 50min(dim lights) under shake.Taking out, the NaOH solution with 5% regulates pH7.0～7.5, accurately adds 10mL toluene, shakes 2min, stands 3-4min layering, and then toluene layer measures at 420nm the absorbance (blank made by toluene) of solution.The mensuration of Selenium In Some Selenium-rich Biological Samples content: accurately draw the sample 0.5mL that digestion process is good, be placed in separatory funnel, operate according to standard curve.The absorbance recorded according to sample, calculates Se content.
Computing formula:
Se content/(μ g/g)=pV/mN
In formula: p is the standard quality concentration being equivalent to selenium/(the μ g/mL) checked in from standard curve；V is the sample volume/mL of toluene extraction gained；M is the quality/g of sample, and N is the volume fraction of sample after the sample volume measured accounts for total constant volume.
Measuring Se content in the Volvariella volvacea (Bull.Ex Franch.) Singer． protein peptide-selenium chelate of preparation is 1526 μ g/g.
Embodiment 2
The first step: by Pleurotus eryngii micronizing, the Pleurotus eryngii after pulverizing with distilled water with w/v
The ratio mixing of 1:60, utilizing 3%NaOH to regulate PH to 11.0, supersound extraction 50min, 120min is extracted in water-bath, temperature 100 DEG C, by filtered through gauze after extraction, filtrate is the Pleurotus eryngii protein solution that alkali carries, and the Pleurotus eryngii protein solution pH to 3.0 carried with hydrochloric acid regulation alkali carries out albumen precipitation, it is centrifuged to obtain protein precipitation, it is washed to neutral recentrifuge, pellet frozen is drying to obtain Pleurotus eryngii albumen, its extraction rate reached 85.75%.
Second step: Pleurotus eryngii protein dissolution making in water albumen quality concentration reach 3.0%, regulates pH to 11.0, temperature is maintained at 60 DEG C, adds 3.0% protease, hydrolyzes 60min, enzyme denaturing, obtains Pleurotus eryngii protein polypeptide complex solution.
3rd step: Pleurotus eryngii protein polypeptide complex solution is added 2.0mol/L sodium selenite solution for 6:5 ratio by volume, stir, regulation pH9.0, sustained response 2.0h under the conditions of 60 DEG C, cools down after reaction, and the removal of impurity is gone in centrifugation, add the ethanol of 5 times of volumes 95%, precipitation stands 12h, centrifugal segregation supernatant, it is thus achieved that Pleurotus eryngii protein peptide-selenium chelate.
4th step: after chelatropic reaction terminates, edible fungus protein peptide-selenium chelate lyophilization.
Use 3,3'-diaminobenzidine colorimetric method for determining Se content.
Weigh Pleurotus eryngii protein peptide-selenium chelate 0.1g, put in 100mL beaker, add 5mL Digestive system, electric furnace digests being water white transparency to sample liquid.After cooling, with the digestion sample liquid of residual in the distilled water wash flask of about 10mL, it is merged in beaker, above-mentioned Digestive system, regulates about pH7.0 with 40%NaOH and 5%NaOH solution, finally by fixed for solution molten to 50mL, to be measured.Bioassay standard curve: take the separatory funnel of 6 125mL, one accurate addition above-mentioned solution 0. 5mL, another 5 be separately added into 2. 0, the selenium standard solution 5 μ g/mL of 4. 0,6. 0,8. 0,10. 0mL, respectively add distilled water to 40mL, with the regulation of 1mol HCl solution to pH2～3, add 0.
The EDTA-2Na solution 2mL of 2M, is subsequently adding the 3.3 '-diaminobenzidine solution (DBA) of 0. 5%
2mL, is placed under 60 DEG C of water-bath 50min (in the environment of dim light) concussion.Taking out, the NaOH solution with 5% regulates pH7.0～7.5, accurately adds 10mL toluene, shakes 2min, stands 3-4min layering, and then toluene layer measures at 420nm the absorbance (blank made by toluene) of solution.The mensuration of Selenium In Some Selenium-rich Biological Samples content: accurately draw the sample 0.5mL that digestion process is good, be placed in separatory funnel, operate according to standard curve.The absorbance recorded according to sample, calculates Se content.
Computing formula:
Se content/(μ g/g)=pV/mN
In formula: p is the standard quality concentration being equivalent to selenium/(the μ g/mL) checked in from standard curve；V is the sample volume/mL of toluene extraction gained；M is the quality/g of sample, and N is the volume fraction of sample after the sample volume measured accounts for total constant volume.
Measuring Se content in the Pleurotus eryngii protein peptide-selenium chelate of preparation is 3848 μ g/g.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent and modification, all should belong to the covering scope of the present invention.
Claims (8)
1. the preparation method of edible fungus protein peptide-selenium chelate, it is characterised in that comprise the steps:
(1), alkali extraction and acid precipitation: utilize alkali extraction-acid precipitation extract edible fungus protein or extract edible fungus protein by ammonium sulfate precipitation method；
(2), enzymolysis: use protease that the edible fungus protein extracted is carried out enzymolysis, enzyme denaturing, prepare edible fungus protein polypeptide complex solution；
(3), prepared by polypeptide-selenium chelate: utilize solid high-temperature mixing method or chelate liquid is legal prepares edible fungus protein peptide-selenium chelate；
(4), edible fungus protein peptide-selenium chelate is carried out lyophilization.
A kind of preparation method of edible fungus protein peptide-selenium chelate, it is characterised in that: described edible fungi includes: Lentinus Edodes, Auricularia, Pleurotus ostreatus, crab flavour mushroom, agrocyb eaegerita, Poria, Phellinus igniarius (L. ex Fr.) Quel., Volvariella volvacea (Bull.Ex Franch.) Singer． , Flammulina velutiper (Fr.) Sing, Pleurotus eryngii, Tremella, one in the edible and medical fungi such as Caulis Bambusae In Taeniam.
nullA kind of preparation method of edible fungus protein peptide-selenium chelate，It is characterized in that: in step (1)，Specifically comprising the following steps that first by edible fungi micronizing of described alkali extraction and acid precipitation，Edible fungi after pulverizing mixes with the ratio of 1:20-60w/v with distilled water，Utilize the NaOH or KOH solution regulation pH to 9.0-11.0 of 0.5-2.0wt.%，Supersound extraction 10-50min，60-120min is extracted in water-bath，Temperature 50-100 DEG C，Filter after extraction，Obtain filtrate，Filtrate is the edible fungus protein solution that alkali carries，The edible fungus protein pH value of solution carried with hydrochloric acid regulation alkali carries out albumen precipitation to 2.5-4.0，3000-5000r/min is centrifuged 5-10min and obtains protein precipitation，It is washed to neutral recentrifuge，Pellet frozen is drying to obtain edible fungus protein.
A kind of preparation method of edible fungus protein peptide-selenium chelate, it is characterized in that: in step (1), described ammonium sulfate precipitation specifically comprise the following steps that first by edible fungi micronizing, edible fungi after pulverizing mixes with the ratio of 1:20-60w/v with distilled water, being gradually added solid ammonium sulfate makes the saturation of ammonium sulfate reach 40-60wt.%, 3000-5000r/min is centrifuged 5-10min, abandon supernatant, obtain edible fungus protein precipitation, utilize dialysis desalination postlyophilization to obtain edible fungus protein.
A kind of preparation method of edible fungus protein peptide-selenium chelate, it is characterized in that: in step (2), the specifically comprising the following steps that to be dissolved in water edible fungus protein of described enzymolysis makes albumen quality concentration reach 1.0-5.0%, regulation pH to 7.0-11.0, temperature is maintained at 40-60 DEG C, adds 1.0-5.0wt.% protease, hydrolyzes 30-90min, enzyme denaturing, obtains edible fungus protein polypeptide complex solution.
The preparation method of a kind of edible fungus protein peptide-selenium chelate the most according to claim 5, it is characterised in that: enzyme described in step (2) uses alkaline protease, neutral protease or compound protease.
A kind of preparation method of edible fungus protein peptide-selenium chelate, it is characterized in that in step (3), it is as follows that described solid high-temperature mixing method prepares concrete operations prepared by polypeptide-selenium chelate: sodium selenite powder and edible fungus protein peptide freeze-dried powder being mixed according to mass ratio 1:6-5:6, high temperature 100-150 DEG C adds hot preparation edible fungus protein peptide-selenium chelate.
A kind of preparation method of edible fungus protein peptide-selenium chelate, it is characterized in that in step (3), described chelate liquid is legal to be prepared specifically comprising the following steps that of polypeptide-selenium chelate and is mixed with 0.5-2mol/L sodium selenite solution by edible fungus protein polypeptide complex solution, both volume ratios are 6:1-6:5, stir, regulation pH is 3.0-10.0, sustained response 1.0-2.0h under the conditions of 30-80 DEG C, cool down after reaction, the removal of impurity is gone in centrifugation, filtrate adds the 75-95%v/v ethanol of 3-5 times of volume, precipitation stands 12-24h, centrifugal segregation supernatant, obtain edible fungus protein peptide-selenium chelate.
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