CN101717808B - Method for preparing rice protein with high dissolubility - Google Patents
Method for preparing rice protein with high dissolubility Download PDFInfo
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- CN101717808B CN101717808B CN2009103117656A CN200910311765A CN101717808B CN 101717808 B CN101717808 B CN 101717808B CN 2009103117656 A CN2009103117656 A CN 2009103117656A CN 200910311765 A CN200910311765 A CN 200910311765A CN 101717808 B CN101717808 B CN 101717808B
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Abstract
The invention relates to a method for preparing rice protein with high dissolubility, and belongs to the technical field of deep machining of plant protein resource. The method uses broken rice as a raw material; and the rice protein separated out by an alkaline extraction and acid precipitation method is subject to working procedures of compound enzyme multi-locus directed modification, graft copolymerization with amylose, spraying, drying and the like to prepare the rice protein with high dissolubility. The nitrogen solubility index NSI of the rice protein prepared by the method is more than or equal to 85 percent; and the rice protein can be widely used as a functional ingredient of a protein beverage, a roasted product, a minced meat product and a nutritional health-care product.
Description
Technical field
A kind of preparation method of high dissolubility rice protein belongs to plant protein resource deep process technology field.
Background technology
Rice protein is a kind of fine plant protein resource, and its essential amino acids content is advised pattern near FAO/WHO, and biological value occupies first in cereal class grain, and digestibility is up to 85%.Simultaneously, be particularly suitable as the base-material of infant and special population nutritive food as a kind of protein resource of hypoallergenic.But the at present domestic rice protein product that does not still have mass-producing production; this is because adopt the rice protein of traditional extraction method preparation to have always that extraction yield is little, purity is not high and problem such as poorly soluble in pH4～10 scopes; now the nitrogen soluble index NSI of Bao Dao rice protein is generally less than 50%; and insoluble protein is very limited in the application of field of food, and high-dissolvability is the prerequisite of other functional propertys such as thickening, foaming, emulsification and gelling.Therefore, thus rice protein is carried out modification to widen its range of application is one of focus of research at present both at home and abroad to improve its solvability.And the domestic rice protein product that does not still have mass-producing production at present.
Summary of the invention
Purpose of the present invention aims to provide a kind of preparation method with high dissolubility rice protein, not only can be fit to large-scale industrial production, resulting rice protein also has higher nitrogen soluble index, can further promote rice and even comprehensive utilization ratio of cracking rice and added value.
The objective of the invention is to realize by following manner:
Preparation process of the present invention comprises: be raw material with rice, carry the heavy isolated rice protein of acid after the prozyme multidigit is put directed modification through alkali, with the polysaccharide graft copolymerization, concentrate, spraying drying obtains; Described prozyme multidigit is put directed modification and is meant rice protein and water sized mixing and makes suspension, after transferring pH to 8.0～9.0, add and account for rice protein suspension 1%～2%w/w prozyme, 45～50 ℃ of following enzymolysis 3～5h, it is centrifugal behind the enzyme to go out, collecting supernatant liquor is the rice protein liquid of enzyme modification, and described prozyme is a Sumizyme MP: trypsinase: papoid=0.90～1.10: 0.60～0.80: 0.40～0.60; Described polysaccharide graft copolymerization is meant transfers pH to 11～13 with rice protein liquid, presses rice protein and 1: 5～7 adding polysaccharide stirrings of polysaccharide quality proportioning, and stir adjust pH to 10～11, and 90～100 ℃ of reactions obtain rice protein and polysaccharide graft copolymer fluid.
Described alkali is carried the heavy rice protein that separates of acid and is meant that rice is added water mill to be starched, and transfers pH to 11.5～12.5 extract at room temperature, 1.5～2.5h, and the centrifuging and taking supernatant liquor is transferred pH to 4.5～5, leaves standstill, and is centrifugal, collecting precipitation, and precipitation is centrifugal after cleaning, the collecting precipitation rice protein.
Rice adds 1: the 5～8w/v of solid-liquid ratio in the water mill slurry process.Solid-to-liquid ratio when precipitation is cleaned is 1: 10～12.
Described simmer down to vacuum concentration, described vacuum tightness are 89～92Kpa, 45～50 ℃ of thickening temperatures, dry matter content 35%～40%.
In the described spray-drying process, 180 ℃ of inlet temperature, 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-3.3Kpa, moisture 3～5%.
Described rice comprise crack rice, rice slag, rice are poor, early rice etc.
Transferring the alkali that adopts in the pH process in the above step is NaOH, and acid is HCl.
The present invention puts directed modification, prepares the rice protein of high-dissolvability with operations such as polysaccharide graft copolymerization, spraying dryings through the prozyme multidigit by alkali being carried the heavy isolated rice protein of acid.Rice protein nitrogen soluble index NSI 〉=85% of the present invention preparation can be widely used as the functional ingredient of protein drinks, baked goods, meat emulsion product and dietary supplements.
The present invention can crack rice and be raw material, alkali is carried the heavy isolated rice protein of acid put directed modification, prepare the high dissolubility rice protein of nitrogen soluble index NSI 〉=85%, remedied the product blank of domestic and international high dissolubility rice protein with operations such as polysaccharide graft copolymerization, spraying dryings through the prozyme multidigit.With the high dissolubility rice protein is that base-material can be developed infant formulas product (high biological value, hypoallergenic, high resolution), health care or functional food ingredient (high biological value, low irritated, anti-oxidant, anticorrosion), bread and cheese batching (high resolution, emulsifying property, whipability), makeup (emulsifying property, moisture retention, sun-proof, anti-oxidant, anticorrosion) etc., can be food, healthcare products and cosmetic field novel protein source is provided, Application Areas is significantly widened, thereby has further promoted the comprehensive utilization ratio and the added value of cracking rice.
Concrete preparation process of the present invention is:
To crack rice is raw material, and the isolated rice protein of alkali extraction and acid precipitation is put directed modification, prepared the rice protein of nitrogen soluble index NSI 〉=85% with operations such as polysaccharide graft copolymerization, spraying drying through the prozyme multidigit.Its processing condition are:
(1) alkali extraction and acid precipitation separates rice protein
[solid-liquid ratio 1: 5～8 (w/v)] → NaOH that cracks rice → defibrination accent pH to 12.0 → extract at room temperature 2h →
The centrifugal 15min of 3000r/min → get supernatant liquor → HCl transfers pH to 4.8 → the leave standstill centrifugal 10min → collecting precipitation of 30min → 4000r/min → cleaning (solid-to-liquid ratio 1: 10～the 12) → centrifugal 10min → collecting precipitation of 4000r/min → separation rice protein
(2) separate rice protein prozyme multidigit and put directed modification
Separate rice protein → by albumen: water=1: 8～10 (w/v) size mixing → rice protein suspension → NaOH accent pH to 8.0～9.0 → adding 1%～2% (w/w) (Sumizyme MP: trypsinase: papoid=0.90～1.10: 0.60～0.80: ℃ enzymolysis 3～5h → 85 ℃/10min rice protein liquid of the centrifugal 15min of enzyme → 3000r/min → collection supernatant liquor → enzyme modification that goes out 0.40～0.60) → 45～50
(3) graft copolymerization of the rice protein of enzyme modification and polysaccharide
It is that 1: 5～7 adding polysaccharide → stirring 30min → adjust pH to 10～11 → stirring 10min → 100 ℃ react 30min → be cooled to 45～50 ℃ → albumen and polysaccharide graft copolymer fluid that the rice protein liquid of enzyme modification → NaOH is transferred pH to 12.0 → stirrings 30min → by rice protein and polysaccharide quality proportioning;
(4) vacuum concentration
Albumen and polysaccharide graft copolymer fluid are cooled to promptly 45～50 ℃ of thickening temperatures, vacuum concentration under vacuum tightness 89～92Kpa, the dry matter content 35%～40% of striking point;
(5) spraying drying
Spraying drying (180 ℃ of inlet temperature, 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-3.3Kpa, moisture 3～5%) → the high dissolubility rice protein
The mensuration of the nitrogen soluble index NSI of the rice protein for preparing by the present invention
Adopt following international method: accurately take by weighing a certain amount of rice protein, be made into the solution of 20mg/mL, HCl or NaOH with 0.1～2.0mol/L transfer pH to preset value, stirring and leaching 30min, the centrifugal 10min of 3000r/min, supernatant liquor is settled to 50mL, gets 5mL and measures total nitrogen content in the supernatant liquor with the semimicro Kjeldahl determination; The same total nitrogen of measuring rice protein with the semimicro Kjeldahl determination.
Rice protein nitrogen soluble index NSI (%)=water-soluble nitrogen/total nitrogen * 100
Beneficial effect of the present invention: the rice protein of traditional extraction method preparation extraction yield is little because of existing, purity is not high and in pH4～10 scopes problem such as poorly soluble, thereby still do not have the high dissolubility rice protein at present both at home and abroad and appear on the market.But high-dissolvability is thickening, bubble, the prerequisite of other functional property such as emulsification and gelling, the present invention is a raw material to crack rice, the isolated rice protein of alkali extraction and acid precipitation is put directed modification through the prozyme multidigit, with the polysaccharide graft copolymerization, operations such as spraying drying prepare the high dissolubility rice protein of nitrogen soluble index NSI=85%, can develop the infant formulas product as base-material, health care or functional foodstuff, bread and cheese, makeup etc., thereby be food, healthcare products and cosmetic field provide novel plant protein source, and have further promoted the comprehensive utilization ratio and the added value of cracking rice.
Process conditions of the present invention is stable, is fit to large-scale industrial production, and the low-value by-product resource of rice productions such as cracking rice, rice slag, rice are poor is reasonably developed.
Embodiment
Following examples are intended to illustrate the present invention rather than limitation of the invention further.
Embodiment 1
(1) alkali extraction and acid precipitation separates rice protein
Crack rice → defibrination [solid-liquid ratio 1: 6 (w/v)] → NaOH transfer pH to 12.0 → extract at room temperature 2h →
The centrifugal 15min of 3000r/min → get supernatant liquor → HCl transfers pH to 4.8 → the leave standstill centrifugal 10min → collecting precipitation of 30min → 4000r/min → cleaning (solid-to-liquid ratio 1: the 10) → centrifugal 10min → collecting precipitation of 4000r/min → separation rice protein
(2) separate rice protein prozyme multidigit and put directed modification
Separating rice protein → by albumen: water=1: 9 (w/v) sizes mixing → rice protein suspension → NaOH accent pH to 8.5 → adding 1.5% (w/w) prozyme (Sumizyme MP: trypsinase: ℃ enzymolysis 4h → 85 ℃/10min rice protein liquid of the centrifugal 15min of enzyme → 3000r/min → collection supernatant liquor → enzyme modification that goes out papoid=1.00: 0.75: 0.50) → 50
(3) graft copolymerization of the rice protein of enzyme modification and polysaccharide
The rice protein liquid of enzyme modification → NaOH is transferred pH to 12.0 → stirrings 30min → ℃ react 30min → be cooled to 45～50 ℃ → albumen and polysaccharide graft copolymer fluid by rice protein and polysaccharide quality proportioning adding in 1: 5 polysaccharide → stirring 30min → adjust pH to 10.5 → stirring 10min → 100
(4) spraying drying
Albumen and polysaccharide graft copolymer fluid → vacuum concentration (vacuum tightness 89～92Kpa, 45 ℃ of temperature, dry matter content 38%) → spraying drying (180 ℃ of inlet temperature, 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-3.3Kpa, moisture 4%) → solubility rice protein NSI 88.5%
Embodiment 2
(1) alkali extraction and acid precipitation separates rice protein
Crack rice → defibrination [solid-liquid ratio 1: 5 (w/v)] → NaOH transfer pH to 12.0 → extract at room temperature 2h →
The centrifugal 15min of 3000r/min → get supernatant liquor → HCl transfers pH to 4.8 → the leave standstill centrifugal 10min → collecting precipitation of 30min → 4000r/min → cleaning (solid-to-liquid ratio 1: the 10) → centrifugal 10min → collecting precipitation of 4000r/min → separation rice protein
(2) separate rice protein prozyme multidigit and put directed modification
Separating rice protein → by albumen: water=1: 10 (w/v) sizes mixing → rice protein suspension → NaOH accent pH to 8.5 → adding 2.0% (w/w) prozyme (Sumizyme MP: trypsinase: the go out rice protein of rice protein liquid (3) enzyme modification of the centrifugal 15min of enzyme → 3000r/min → collection supernatant liquor → enzyme modification and the graft copolymerization of polysaccharide of ℃ enzymolysis 4.5h → 85 ℃/10min papoid=1.10: 0.70: 0.45) → 50
The rice protein liquid of enzyme modification → NaOH is transferred pH to 12.0 → stirrings 30min → ℃ react 30min → be cooled to 45～50 ℃ → albumen and polysaccharide graft copolymer fluid by rice protein and polysaccharide quality proportioning adding in 1: 6 polysaccharide → stirring 30min → adjust pH to 11.0 → stirring 10min → 100
(4) spraying drying
Albumen and polysaccharide graft copolymer fluid → vacuum concentration (vacuum tightness 89～92Kpa, 48 ℃ of temperature, dry matter content 40%) → spraying drying (180 ℃ of inlet temperature, 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-3.3Kpa, moisture 3.5%) → solubility rice protein NSI 89.5%.
Claims (11)
1. the preparation method with high dissolubility rice protein is characterized in that preparation process comprises: be raw material with rice, propose acid through alkali and sink isolated rice protein after the prozyme multidigit is put directed modification that with the polysaccharide graft copolymerization, concentrate, spraying drying obtains; Described prozyme multidigit is put directed modification and is meant rice protein and water sized mixing and makes suspension, after transferring pH to 8.0～9.0, add and account for rice protein suspension 1%～2%w/w prozyme, 45～50 ℃ of following enzymolysis 3～5h, it is centrifugal behind the enzyme to go out, collecting supernatant liquor is the rice protein liquid of enzyme modification, and described prozyme is a Sumizyme MP: trypsinase: papoid=0.90～1.10: 0.60～0.80: 0.40～0.60; Described polysaccharide graft copolymerization is meant transfers pH to 11～13 with rice protein liquid, presses rice protein and 1: 5～7 adding polysaccharide stirrings of polysaccharide quality proportioning, and stir adjust pH to 10～11, and 90～100 ℃ of reactions obtain rice protein and polysaccharide graft copolymer fluid.
2. a kind of preparation method according to claim 1 with high dissolubility rice protein, it is characterized in that, described alkali is carried the heavy rice protein that separates of acid and is meant that rice is added water mill to be starched, and transfers pH to 11.5～12.5 extract at room temperature, 1.5～2.5h, the centrifuging and taking supernatant liquor, transfer pH to 4.5～5, leave standstill, centrifugal, collecting precipitation, it is centrifugal that precipitation is cleaned the back, the collecting precipitation rice protein.
3. a kind of preparation method with high dissolubility rice protein according to claim 2 is characterized in that, rice adds 1: the 5～8w/v of solid-liquid ratio in the water mill slurry process.
4. a kind of preparation method with high dissolubility rice protein according to claim 2 is characterized in that, the solid-to-liquid ratio when precipitation is cleaned is 1: 10～12.
5. the preparation method of a kind of high dissolubility rice protein according to claim 1 is characterized in that described simmer down to vacuum concentration, and described vacuum tightness is 89～92Kpa, 45～50 ℃ of thickening temperatures, dry matter content 35%～40%.
6. a kind of preparation method with high dissolubility rice protein according to claim 5 is characterized in that in the described spray-drying process, 180 ℃ of inlet temperature, and 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-3.3Kpa, moisture 3～5%.
7. according to each described a kind of preparation method of claim 1-6, it is characterized in that described rice comprises cracks rice or the rice slag with high dissolubility rice protein.
8. according to each described a kind of preparation method of claim 1-6, it is characterized in that described rice comprises that rice is poor with high dissolubility rice protein.
9. according to each described a kind of preparation method of claim 1-6, it is characterized in that described rice comprises early rice with high dissolubility rice protein.
10. according to each described a kind of preparation method with high dissolubility rice protein of claim 1-6, it is characterized in that transferring the alkali that adopts in the pH process is NaOH, acid is HCl.
11. a kind of preparation method with high dissolubility rice protein according to claim 1 is characterized in that preparation process is:
(1) alkali is carried the heavy rice protein that separates of acid
Crack rice → defibrination: 1: 5～8w/v of solid-liquid ratio → NaOH transfers pH to 12.0 → centrifugal 15min of extract at room temperature 2h → 3000r/min → get supernatant liquor → HCl to transfer pH to 4.8 → the leave standstill centrifugal 10min → collecting precipitation of 30min → 4000r/min → cleaning: the centrifugal 10min → collecting precipitation of 1: 10～12 → 4000r/min of solid-to-liquid ratio → separation rice protein;
(2) separate rice protein prozyme multidigit and put directed modification
Separate rice protein → by albumen: water=1: 8～10w/v sizes mixing → rice protein suspension → NaOH transfers pH to 8.0～9.0 → adding 1%～2%w/w prozyme: Sumizyme MP: trypsinase: papoid=0.90～1.10: 0.60～0.80: the go out rice protein liquid of the centrifugal 15min of enzyme → 3000r/min → collection supernatant liquor → enzyme modification of 0.40～0.60 → 45～50 ℃ of enzymolysis 3～5h → 85 ℃/10min;
(3) graft copolymerization of the rice protein of enzyme modification and polysaccharide
It is that 1: 5～7 adding polysaccharide → stirring 30min → adjust pH to 10～11 → stirring 10min → 100 ℃ react 30min → albumen and polysaccharide graft copolymer fluid that the rice protein liquid of enzyme modification → NaOH is transferred pH to 12.0 → stirrings 30min → by rice protein and polysaccharide quality proportioning;
(4) vacuum concentration
Albumen and polysaccharide graft copolymer fluid are cooled to promptly 45～50 ℃ of thickening temperatures, vacuum concentration under the vacuum tightness of 89～92Kpa, the dry biomass content 35%～40% of striking point;
(5) spraying drying
180 ℃ of inlet temperature, 80 ℃ of air outlet temperatures, drying chamber pressure-1.9～-spissated albumen and polysaccharide graft copolymer fluid are carried out spraying drying under the condition of 3.3Kpa, can obtain moisture content and be 3～5% high dissolubility rice protein.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102150736A (en) * | 2011-03-26 | 2011-08-17 | 山东大学威海分校 | Method for extracting protein of rice residue |
| CN102165989B (en) * | 2011-04-12 | 2012-10-03 | 江南大学 | Preparation method of soluble rice protein |
| CN102212107B (en) * | 2011-04-12 | 2013-01-02 | 江南大学 | Rice protein polypeptide and preparation method thereof |
| CN104431282A (en) * | 2013-09-18 | 2015-03-25 | 盘锦和田食品有限公司 | Method for preparing rice protein powder |
| CN104404115B (en) * | 2014-11-11 | 2018-05-04 | 河南旭百瑞生物科技股份有限公司 | A kind of production method of high-purity enzymolysis rice protein powder |
| CN104789615A (en) * | 2015-04-03 | 2015-07-22 | 宝鸡市虹源生物科技有限公司 | Co-production method for producing black rice anthocyanins, black rice syrup and black rice protein from black rice with biological enzyme method |
| CN109810268B (en) * | 2017-11-20 | 2022-01-14 | 长沙理工大学 | Method for constructing nano or micron-scale complex coacervate by using rice protein and Arabic gum |
| CN109123065B (en) * | 2018-07-05 | 2020-10-20 | 江南大学 | Preparation method of soluble sugar rack rice protein |
| CN113995020B (en) * | 2021-11-16 | 2023-08-04 | 江南大学 | Plant high-protein rice milk and preparation method thereof |
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