CN106070009B - A kind of SD rat production method - Google Patents
A kind of SD rat production method Download PDFInfo
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- CN106070009B CN106070009B CN201610423303.3A CN201610423303A CN106070009B CN 106070009 B CN106070009 B CN 106070009B CN 201610423303 A CN201610423303 A CN 201610423303A CN 106070009 B CN106070009 B CN 106070009B
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of SD rat production methods, it is the ratio progress first round mating of female mice and male mouse according to 1:2, male mouse is separated into raising with female mice after female mice pregnancy, pregnancy female mice raise to farrowing after 1 ~ 2 day, carry out the second wheel according to the ratio of 1:2 with male mouse again mate, male mouse separated into raising with female mice after female mice pregnancy, the female mice that is pregnant raise to farrowing after 1 ~ 2 day, again with male mouse according to 1:2 ratio carry out third round mate, and so on to female mice eliminate；Female mice feeding warp60The breeding feed of Co radiation sterilization, free water, the breeding feed includes following component: crude protein, crude fat, crude fibre, coarse ash, calcium, phosphorus, nitrogen carbamylglutamic acid, lysine, methionine, cystine, vitamin, water, and surplus is glucose.The present invention is more than the matching method of female mice using male mouse by improving to Circulate mating method, cooperates breeding feed, can significantly improve the reproductive efficiency and newborn mouse quality of SD rat.
Description
Technical field
The present invention relates to experimental animal production technical fields, and in particular to a kind of SD rat production method.
Background technique
Rat and mouse as most common experimental animal, be widely used in medicine, pharmacy, biology, veterinary science,
All kinds of basic research such as embryology, science of heredity and application field.In biomedical zoopery, utilization rate is up to 80% or so.
The production of standardized assay rat and mouse, in addition to it must carry out microbiology, science of heredity, environmental Kuznets Curves, supply
The feed of nutritional ingredient proportion science is to maintain animal health, improves a critical of zoopery quality.With section
The development of skill cause, the requirement to rat and mouse quality and quantity step up, and experimental animal feeding quality comes into state
Family's standardized management system.
SD rat is nineteen twenty-five cultivated as the farm the Sprague Dawley Wistar rat in the U.S. made of one it is white
Change closed colony rat, has a sweet temper, and more stronger than the adaptability of Wistar rat and disease resistance, be more important experiment
Rat kind, but its price is more expensive, the scale raised at present does not have Wistar rat extensive.SD rat is common do nutrition and
The research of endocrine system.It is mostly used for safety testing and nutrition research related with growth and development.The strain is to sex hormone
Sensitivity has stronger resistance to respiratory disease.It is widely used in pharmacology, toxicity, drug effect and GLP experiment.
Medical experiment is often more stringent to experimental animal weight or age in days requirement, therefore according to experimental animal application plan
It is extremely important to formulate corresponding breeding plan.For experimental animal professional production mechanism, reduce production cost, planned production,
Constant rate of production, the relationship of balancing supply with demand are to need to face and solve the problems, such as.
It is still at present to be carried out according to " SD rat feeding operating instruction " for the propagating and breeding of SD rat." Jiangxi herding
Animal doctor's impurity " a kind of a kind of the 5th phase page 8 in 2004 " the effective rat propagation method " propagation method that describes SD rat, be
It is compared according to female the matching of 1 hero 2, Circulate mating, rat nest litter size is between 9.16 ~ 11.84." experimental animal is cured compared with
Learn " 2010.30(1): 55-57 describes " comparison of two kinds of modess of reproduction to SD rat production performance ", it is believed that male and female 6:1 circulation
Mating method can effectively improve and be separated from milk survival rate, improve the production efficiency of newborn mouse, can be effectively controlled animal population quantity and production number
Amount, is suitable for the large-scale production of rat.
In general, the reproductive performance with animal germline of large and small mouse, environmental condition, nutritive water equality factors are related.
The height of dietary nutritional standard directly affects the fertility of animal, scientific nutrition-allocated proportion, especially compound ammonia
Base acid, microelement, vitamin supplement be guarantee just with farrowing rate, average litter size, averagely be separated from milk number, be separated from milk survival rate
Premise.Protein lacks, and will cause mouse sperm, semen volume reduction, reduces the pregnancy rate of female mice, while increasing embryo's Deaths
Rate；Vitamin, minerals are inseparable with the synthesis of vivo protein, body metabolism, and vitamin D deficiency influences calcium phosphorus
When the deficiencies of metabolism, calcium phosphorus, it can cause reproductive capability that obstacle occurs；Vitamin E is insufficient, can cause interrupted pregnancy Pregnancy, stillborn foetus, weak tire
Or recessive miscarriage.But protein, fat or carbohydrate are excessive, then cause mouse fat accumulation, follicular epithelium steatosis, and
It causes to be not easy heat, influences reproductive capacity.
The breeding that the existing SD rat production management of offer in to(for) SD rat nutrition still uses large and small mouse general
Grain feed.
Summary of the invention
It is an object of the invention to be directed to the physiological characteristic of SD rat, on the basis of Circulate mating method, one kind is provided more
Add scientific and effective SD rat production method, increase yield while to guarantee that implementation specification is consistent, reduce production cost, shortens
Production cycle.
A kind of SD rat production method provided by the invention, be selection health female mice and male mouse according to 1:2 ratio into
Male mouse is separated raising with female mice after confirmation female mice pregnancy by the mating of the row first round, the female mice that is pregnant raise to farrowing after 1 ~ 2 day, then
The second wheel is carried out according to the ratio of 1:2 with male mouse to mate, and male mouse is separated into raising with female mice after confirmation female mice pregnancy, be pregnant female mice
Raising to farrow after 1 ~ 2 day, then with male mouse according to 1:2 ratio carry out third round mate, and so on to female mice eliminate；
Female mice feeding warp60The breeding feed of Co radiation sterilization, free water, the breeding feed includes following component:
Crude protein 25.0 ~ 27.9%, crude fat 6.8 ~ 7.2%, crude fibre 5.0 ~ 6.0%, coarse ash 4.8 ~ 5.1%, calcium 1.7 ~
1.9%, phosphorus 0.90 ~ 0.95%, nitrogen carbamylglutamic acid 0.02%, lysine 1.0 ~ 1.2%, methionine 0.35 ~ 0.45%, cystine
0.2 ~ 0.3%, 11000 ~ 13000mg/kg of vitamin, water 9.0 ~ 11.0%, surplus are glucose.
The male mouse of raising and female mice match comparative example, are conducive to that female mice is induced to receive mating in estrus, and ovulation rate is pregnant,
Female mice pregnancy rate can be significantly improved, increasing male female ratio can not become pregnant the problem of delaying production for a long time to avoid female mice, base
It can all become pregnant in this upper one week.There is heat performance, in the case where nutritional sufficiency, 1 ~ 2 within 1 day or so after female mice production
It is become pregnant next time, also can effective improving production efficiency, while in order to avoid female mice gestation and lactation bring pair
Heavy burden needs to feed special breeding feed to provide enough nutritional needs.The study found that increasing nitrogen carbamylglutamic acid
Cooperate vitamin (folate content is greater than 0.20ppm in vitamin, and there is no limit for other vitamin ingredients contents), compared to independent
Nitrogen carbamylglutamic acid is added, can guarantee preparation and embryo's successful implantation in the preceding uterus of early embryonic development, implantation, improves SD
The average nest litter size and litter weight of rat.
Preferably, above-mentioned SD rat production method further includes 205 ~ 215mg/kg of zinc in the breeding feed.Zinc is as micro-
Secondary element plays a significant role in growth of animal and reproductive process, previous studies have shown that high zinc content (400mg/ in feed
Kg or more) rat appetite can be caused to lower, mostly dynamic, irritated, phenomena such as hair is sparse, easily broken, matt, growth retardation, body
Decline again, has an adverse effect to immune organ such as thymus gland and spleen.Therefore, the Zn content in rat feed is usually in 50 mg/
The kg(upper limit is 150mg/kg).But the study find that being further added by slightly higher content after increasing nitrogen carbamylglutamic acid in breeding feed
Zinc can dramatically increase newborn mouse weight before ablactation, while this may be female mice milk abundance caused by newborn mouse appetite increase.
Preferably, above-mentioned SD rat production method, the dosage of each ingredient in the breeding feed are as follows:
Crude protein 26.5%, crude fat 7.0%, crude fibre 6.0%, coarse ash 5.1%, calcium 1.8%, phosphorus 0.94%, nitrogen carbamyl
Glutamic acid 0.02%, lysine 1.0%, methionine 0.35%, cystine 0.3%, zinc 215mg/kg, vitamin 12 700mg/kg, water
11.0%, surplus is glucose.
Preferably, above-mentioned SD rat production method, male mouse, which increases, raises peanut.Peanut is required rich in the spermiogenesis tails such as arginine
Nutritional ingredient can be improved male mouse sperm quality.
Preferably, above-mentioned SD rat production method, the hero mouse and female mice more than 85 ages in days, male mouse weight 290 ~
310g, female mice weight 215g ~ 225g.
Preferably, any description above SD rat production method, 23 ~ 25 DEG C of feeding environment temperature, relative humidity 45 ~
55%, 85 decibels of noise hereinafter, ammonia density 20ppm hereinafter, 18 ~ 20 time/hour of ventilation.
Preferably, above-mentioned SD rat production method, the mating using the method that mates at night, 8 points of morning with
Before check whether pregnancy.SD rat mostly ovulates after night heat, heat, therefore it is higher to mate pregnancy rate at night, and reduces
The male unnecessary movement consumption of mouse.
Preferably, above-mentioned SD rat production method, the female mice are eliminated after breeding 5 tires.SD rat female mice is in preceding 5 tire
Breeding climax is in more apparent downward trend since the 6th tire, from the economic viewpoint, continues to use fertility decline
Rat will lead to cost submission, therefore the 5th fetus mouse is separated from milk rear female mice and preferably eliminates.
SD rat production method of the invention has the advantages that
By improving to Circulate mating method, it is more than the matching method of female mice using male mouse, guarantee is quickly become pregnant in short term,
Achieve the effect that same batch mass production, in order to keep female mice and newborn mouse nutritional need, enhances physical fitness, prepare special formulation
Breeding feed, the reproductive efficiency and newborn mouse quality of SD rat can be significantly improved, shorten the production cycle.
Specific embodiment
Below with reference to embodiment, further the present invention will be described so that those skilled in the art may be better understood
The present invention is simultaneously practiced, but illustrated embodiment and is not constituted a limitation of the invention.
Such as non-specified otherwise, reagent, instrument used in embodiment etc. is customary commercial.
Embodiment 1
Select healthy female mice and the male mouse of barrier environment raising 100 total, wherein female mice 80, be 90 ages in days with
On, weight is between 216 ~ 225g, average weight 219.8g；Male mouse 20, is 85 ages in days or more, weight 290 ~ 310g it
Between, average weight 301.6g.
It should be that health is disease-free as the female mice of parent and male mouse, coat is dense glossy and clings to the body；Eyes are bright
Bright to have mind, breathing is normal, and quadruped locomotion is strong rapidly, and tail is well-proportioned without falling off, and head is broad, and neck length is moderate, back
Portion is broad straight, and abdomen is fastened.Male testicualr development is normal, and magnetic nipple is obvious.
The feeding environment of female mice and male mouse: 23 ~ 25 DEG C of temperature, relative humidity 45 ~ 55%, 85 decibels of noise hereinafter, ammonia density
20ppm is hereinafter, 18 ~ 20 time/hour of ventilation, illumination condition are illumination in 12 hours 12 hours dark alternatings.
Female mice and male mouse feed warp60The breeding feed of Co radiation sterilization, male mouse, which increases, raises peanut, is freely eaten and drinks water,
The breeding feed includes following component:
Crude protein 26.5%, crude fat 7.0%, crude fibre 6.0%, coarse ash 5.1%, calcium 1.8%, phosphorus 0.94%, nitrogen carbamyl
Glutamic acid 0.02%, lysine 1.0%, methionine 0.35%, cystine 0.3%, zinc 215mg/kg, vitamin 12 700mg/kg, water
11.0%, surplus is glucose.
Drinking water is the acidifying water of pH 2.5 ~ 2.8.
The additive amount of feed, which should try to control, there is not residue between food twice, and drinking-water will guarantee successively, use
Drinking bottle water supply is changed weekly water 2 ~ 3 times, also be can be adjusted according to the actual situation.
Every 10 a batch of female mice, every independent mouse rearing in box are supported, and 2 male mouse are put into every female mice case, carry out first
Wheel mating；It checks every other day once, male mouse is taken out after confirmation female mice pregnancy, is put into second batch female mice case, carry out the second wheel and mate；
It checks every other day once, male mouse is taken out after confirmation female mice pregnancy, third batch female mice case is put into, third round mating is carried out, with such
It pushes away.It mates preferably at night, male mouse is put into female mice case, uses vaginal smear method to check for essence before 8 points of morning
Son has being considered as sperm to be pregnant successfully.
Female mice after pregnancy is individually raised to farrowing, and after farrowing one day, is put into male mouse in the ratio of sex ration 1:2, into
The next tire of row is bred, and newborn mouse is separated from milk after 21 days, and female mice is eliminated after producing 5 tires.
Newborn mouse investigates thoroughly gender in two weeks after being separated from milk and male and female are separately raised.
By this improved Circulate mating method, it can be obviously shortened the interval between parity, conventional method needs 42 days,
Method of the invention can shorten general two weeks or so, and due to the adjustment in nutrition, newborn mouse litter weight, survival rate are also mentioned
It is high.
The above description of the embodiments is only for helping to understand the core idea of the present invention.It should be pointed out that for this technology
For the those of ordinary skill in field, without departing from the principle of the present invention, several improvement can also be carried out to the present invention
And modification, these improvements and modifications also fall within the scope of protection of the claims of the present invention.
Claims (8)
1. a kind of SD rat production method, which is characterized in that the female mice of health and male mouse is selected to carry out first according to the ratio of 1:2
Male mouse is separated raising with female mice after confirmation female mice pregnancy by wheel mating, the female mice that is pregnant raise to farrowing after 1 ~ 2 day, then with male mouse
Carry out the second wheel mating according to the ratio of 1:2, male mouse separated into raising with female mice after confirmation female mice pregnancy, the female mice that is pregnant raise to
1 ~ 2 day after farrowing, then with male mouse according to 1:2 ratio carry out third round mate, and so on to female mice eliminate；
Female mice feeding warp60The breeding feed of Co radiation sterilization, free water, the breeding feed includes following component:
Crude protein 25.0 ~ 27.9%, crude fat 6.8 ~ 7.2%, crude fibre 5.0 ~ 6.0%, coarse ash 4.8 ~ 5.1%, calcium 1.7 ~ 1.9%,
Phosphorus 0.90 ~ 0.95%, nitrogen carbamylglutamic acid 0.02%, lysine 1.0 ~ 1.2%, methionine 0.35 ~ 0.45%, cystine 0.2 ~
0.3%, 11000 ~ 13000mg/kg of vitamin, water 9.0 ~ 11.0%, surplus are glucose.
2. SD rat production method according to claim 1, which is characterized in that further include in the breeding feed zinc 205 ~
215mg/kg。
3. SD rat production method according to claim 2, which is characterized in that the dosage of each ingredient in the breeding feed
Are as follows:
Crude protein 26.5%, crude fat 7.0%, crude fibre 6.0%, coarse ash 5.1%, calcium 1.8%, phosphorus 0.94%, nitrogen carbamyl paddy ammonia
Acid 0.02%, lysine 1.0%, methionine 0.35%, cystine 0.3%, zinc 215mg/kg, vitamin 12 700mg/kg, water
11.0%, surplus is glucose.
4. SD rat production method according to claim 1, which is characterized in that male mouse, which increases, raises peanut.
5. any SD rat production method according to claim 1 ~ 4, which is characterized in that the hero mouse and female mice were on 85th
It is more than age, 290 ~ 310g of male mouse weight, female mice weight 215g ~ 225g.
6. any SD rat production method according to claim 1 ~ 4, which is characterized in that 23 ~ 25 DEG C of feeding environment temperature,
Relative humidity 45 ~ 55%, 85 decibels of noise hereinafter, ammonia density 20ppm hereinafter, 18 ~ 20 time/hour of ventilation.
7. SD rat production method according to claim 1, which is characterized in that the mating is using the side mated at night
Method, 8 points of morning check whether pregnancy in the past.
8. SD rat production method according to claim 1, which is characterized in that the female mice is eliminated after breeding 5 tires.
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