CN112088833A - Feeding method capable of timely and accurately detecting pregnancy of experimental mice - Google Patents
Feeding method capable of timely and accurately detecting pregnancy of experimental mice Download PDFInfo
- Publication number
- CN112088833A CN112088833A CN201911134043.8A CN201911134043A CN112088833A CN 112088833 A CN112088833 A CN 112088833A CN 201911134043 A CN201911134043 A CN 201911134043A CN 112088833 A CN112088833 A CN 112088833A
- Authority
- CN
- China
- Prior art keywords
- mice
- day
- female
- mouse
- experimental
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000699670 Mus sp. Species 0.000 title claims abstract description 91
- 230000035935 pregnancy Effects 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000699666 Mus <mouse, genus> Species 0.000 claims abstract description 42
- 238000002474 experimental method Methods 0.000 claims description 46
- 238000009395 breeding Methods 0.000 claims description 18
- 230000001488 breeding effect Effects 0.000 claims description 17
- 230000003044 adaptive effect Effects 0.000 claims description 2
- 238000010171 animal model Methods 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 8
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 description 17
- 230000037396 body weight Effects 0.000 description 16
- 208000005189 Embolism Diseases 0.000 description 15
- 230000004584 weight gain Effects 0.000 description 12
- 235000019786 weight gain Nutrition 0.000 description 12
- 239000006216 vaginal suppository Substances 0.000 description 10
- 229940120293 vaginal suppository Drugs 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 6
- 241000700159 Rattus Species 0.000 description 5
- 230000000007 visual effect Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000011179 visual inspection Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 206010000210 abortion Diseases 0.000 description 2
- 231100000176 abortion Toxicity 0.000 description 2
- 210000003679 cervix uteri Anatomy 0.000 description 2
- 230000012173 estrus Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000009597 pregnancy test Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000001215 vagina Anatomy 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000011120 smear test Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 210000003905 vulva Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A feeding method capable of timely and accurately detecting pregnancy of an experimental mouse belongs to the technical field of experimental animal pregnancy diagnosis and feeding. The invention adopts a scheme of five-day circulating cage-closing feeding combined with female mouse weight observation, namely, the female and male mice are fed in a cage-closing manner for 1 day every 5 days, and the female mouse with the weight increased by 3.48 +/-0.47 g is determined as the pregnant mouse, so that the purposes of early identifying the pregnant mouse and simplifying the experimental process are realized.
Description
Technical Field
The invention belongs to the technical field of experimental animal pregnancy diagnosis and feeding.
Background
The experimental animal is an animal engaged in scientific research, teaching, production, detection and scientific experiments thereof, is widely applied to aspects of biomedicine, medicine research and development, teaching experiments, biological detection and the like, and plays an important role in life science research. Because the gene similarity of the mouse and the human is 99 percent, and the breeding of the mouse and the rat are relatively cheap and the breeding is rapid. At present, about 9 mice or rats are used in 10 experimental animals, and the application fields include immunology, oncology and physiology. Pathology, embryology, toxicology, etc., and the application in neuroscience is increasing, and experimental mice also become the pronouns of experimental animals.
The breeding of experimental mice is the key of the production and experimental process of experimental animals. In the breeding process of the experimental mice, the pregnancy days and the pregnancy time are decisive factors for determining the cage feeding time of the experimental mice and the preparation of the experimental pregnant mice. Accurate pregnancy diagnosis can enable experimental animal manufacturers and researchers to master accurate pregnancy time, feed space is arranged more reasonably and effectively, missing detection is further reduced, the utilization rate of experimental animals is improved, and feeding cost is reduced. The current commonly used methods for diagnosing pregnancy of laboratory mice include visual vaginal embolus detection and vaginal sperm smear examination.
A vaginal suppository detection method: the vaginal suppository is a white serous substance formed after 4-5 min mating of mice, is blocked in the cavity from vagina to cervix, and generally falls off automatically 12-24h after mating. The vaginal embolism detection method is to detect whether vaginal embolism is detected in the morning of the next day after mice are housed in cages as a sign of mating and pregnancy, and is mainly determined by visual inspection and detection at present. The visual observation is directly observed by eyes, the detection needs to hold the detection rod to lightly touch the mouse vaginal orifice, if the touch is obviously resisted, the vaginal suppository is shown, and if the touch is not resisted, the detection rod can be inserted without resistance, the vaginal suppository is not shown. The method is simple and convenient, but because the size of the pessaries of the rats is different, the pessaries protrude from the vaginal opening to be easily observed, and the pessaries protrude from the vaginal opening to be carefully checked if the pessaries are small in the cervix, the diagnosis is difficult to confirm only by visual inspection, the probability of missing detection is high, the feeding cost is increased, and the production waste is caused. The detection rod for detection is mostly self-made, and is easy to generate great stimulation to female mice in the detection process. Pregnant mice and newborn mice caused by missed detection also need to be treated because relevant indexes cannot be determined, and negative influence is caused on animal ethics.
Vaginal sperm smear test: and (3) slightly screwing the cotton swab stained with the physiological saline into the vagina of the female mouse in the morning of the next day after the mouse is closed, taking out the female mouse after rotating for a circle, smearing the cotton swab, and performing a papanicolaou staining microscopic examination. Although the method has high accuracy, the procedure is complicated. In the actual large-scale production and experiment process, a large amount of time is consumed due to the large number of animals, and the method is not practical.
The visual vaginal embolus detection method and the vaginal sperm smear inspection method have higher requirements on capturing and observing techniques of experimenters involved in the field of experiments for the first time, and increase the possibility that the experimenters involved in the experiments for the first time are accidentally injured by animals. The assistance of laboratory tests and the like further increase the cost of experiment time and money and the like, and the possibility of causing more experiment loss once the pregnancy date is determined incorrectly.
Disclosure of Invention
In order to solve the defect that the pregnant mouse cannot be quickly, simply and accurately identified under the existing conditions, the invention provides a breeding method which can conveniently, timely and accurately detect the pregnancy of the experimental mouse.
The invention comprises the following steps:
1) c57BL/6JA male and female mice 8-16 weeks old are bred in cages for 7 days in an adaptive manner;
2) breeding male and female mice in a cage for 1 day;
3) breeding the male and female mice in cages for 4 days; marking the female mice respectively on the day 1 of cage breeding, and recording the weight of each female mouse respectively;
4) breeding male and female mice in a cage randomly for 1 day;
5) breeding the male and female mice in cages for 4 days; determining female mice with the weight increased by 3.48 +/-0.47 g as pregnant mice on the day 4 of cage feeding, and separating and introducing the pregnant mice into a pregnancy period experiment;
6) the operation is cycled according to the steps 4) and 5).
The invention adopts a five-day circulating cage-closing and cage-dividing feeding combined female mouse weight observation scheme, namely, the female and male mice are fed in a cage-closing manner for 1 day every 5 days, and the female mouse with the weight increased by 3.48 +/-0.47 g is determined as the pregnant mouse, so that the purposes of early identifying the pregnant mouse and simplifying the experimental process are realized.
The method is suitable for detecting the pregnancy of the experimental mouse, is particularly suitable for novices involved in experiments, can timely and accurately identify the pregnancy date of the pregnant mouse by using the weight change observation of the mouse, reduces the using amount of experimental animals and reasonably arranges the experimental process.
Furthermore, when the invention is bred in cages, male and female mice are randomly bred in a ratio of 2: 1.
Detailed Description
1. Preparation of the experiment: selecting a plurality of 8-16 week-old C57BL/6JA mice according to experimental requirements, and adaptively feeding the male and female mice in cages for 7 days.
2. Day 1 of the experiment: the male and female mice were randomly housed in a 2: 1 ratio.
3. Experiment 2 to 5 days: the male and female mice are raised in cages.
And female mice were individually labeled on day 2 of the experiment and the body weight of each female mouse was recorded.
Generally, if a vaginal embolism is observed by the vaginal embolus test method, the female mouse can be established as EA0.5d (since many mice cross at night).
4. Day 6 of the experiment: male and female mice were again randomly housed in a 2: 1 ratio.
5. Experiment 7 to 10 days: and breeding the male and female mice in cages.
Similarly, if a vaginal embolism is observed by the pessary method, the female mouse can be drawn up to EB0.5d.
6. Experiment day 11: the body weight of each female mouse was recorded separately.
In the case of no weight gain or only 1g fluctuation and below, the male and female mice were randomly housed again at a ratio of 2: 1.
Female mice with a body weight increase of 3.48 ± 0.47g before 9 days were identified as pregnant mice and recorded as ea9.5d, and pregnant mice were divided and included in each relevant pregnancy test.
7. Experiment 12 to 15 days: and breeding the male and female mice in cages.
Similarly, if a vaginal embolism is observed as by the vaginal embolus test on day 12, the rat can be assigned an EC0.5d.
8. Day 16 of the experiment: the body weight of each female mouse was recorded separately.
In the case of no weight gain or only 1g fluctuation and below, the male and female mice were randomly housed again at a ratio of 2: 1.
Female mice with a body weight increase of 3.48 + -0.47 g before 9 days were identified as pregnant mice and recorded as EB9.5d, and pregnant mice were divided and included in each relevant pregnancy test.
9. Experiment day 17: and (5) breeding male mice in cages.
Similarly, if a vaginal embolism is observed as by the vaginal embolus test on day 17, the rat can be assigned an ED0.5d.
Therefore, the female mouse and the male mouse are combined once every 5 days, the experimental process can be simplified, an ideal experiment is carried out in the early days, and the experimental pregnant mouse with the expected determined schedule is obtained.
The experimental animal feeding center mostly adopts vaginal suppository visual observation to observe the production of experimental mice, determines the pregnancy period of the experimental mice, and the unexpected production can be used as breed conservation and the like for feeding. Most experimental mice used by experimental researchers are unique in variety, small in number, short in experimental period requirement and high in time cost and economic cost of the experimenters. Typically, the blastocyst begins to be implanted about 5 days after the mouse is born and fertilized, and the gestational period is 19-21 days. After sexual maturity, the estrus cycle is 4-5 days. If a professional selects an estrus mouse with ruddy or congested vulva and swelling and open vaginal orifice to enter the first day of caging before caging, the pregnancy rate of the experiment can be increased. If the day of vaginal suppository is E0.5d, the day of production is mostly E19.5d.
The experimenter who enters the experiment for the first time is difficult to grasp the mouse and identify the vaginal embolus, and is easy to be accidentally injured by the experimental animal. And the experimental report of the accuracy rate of identifying the pregnancy of the mouse by only relying on the vaginal embolus is only about 60 percent. The identification accuracy rate reaches 100 percent by the method. Is mainly suitable for recognizing pregnant mice E9.5d and later. Compared with the traditional experimental arrangement, the experimental schedule is reduced by at least half.
Example (c):
mice that had been visually inspected for negative emboli, presumably pregnant, were used as the visual negative emboli group. The hypothetical pregnant experimental mice whose body weight increased on day 10 were isolated by male and female mice after cage-closing were a ten-day pregnant body weight increased group. The method of the invention is an invention group.
Experiment one: and observing the weight increase of the mice during pregnancy.
Table 1:
| number of mice | Weight change (unit: g) | |
| E0.5d | 6 | 19.167±1.019 |
| E4.5d | 6 | 19.433±1.035 |
| E8.5d | 6 | 21.917±0.911 |
| E9.5d | 6 | 22.65±0.742 |
| E12.5d | 6 | 25.467±1.199 |
| E13.5d | 6 | 26.65±1.433 |
Table 2:
| number of mice | Weight change (unit: g) | |
| 4d weight gain | 6 | 0.267±0.103 |
| 5d weight gain | 6 | 0.816±0.133 |
| 9d weight gain | 6 | 3.483±0.467 |
Experiment two: 10 pregnant mice which are supposed to be pregnant are selected from each group, and the accuracy rate of pregnancy is observed.
Table 3: successful recognition rate of pregnant mouse
Experiment three: assume that 32 females were enrolled for 30 days into the experiment.
Visual inspection of the vaginal suppository groups: the number of possible pregnancies per closure was 50%. The experimental personnel visually observe the vaginal suppository group, take daily observation of vaginal suppository as an example, and find that the vaginal suppository is independently raised in cages. The 30-day experiment totals the number of mice that can be entered into the correct experimental time E9.5d. The mice were kept in the cages on the 1 st day of the experiment, 16 mice with vaginal emboli were observed on the 2 nd day of the experiment, and the mice remained in the cages. On day 3 8 mice with vaginal emboli were observed and the remainder were kept in cages. On day 4, only pessary mice were seen and observed, and the remainder were continued to be housed. By analogy, the test day 7 may be totally included in the observation group, but the vaginal embolism detection shows that the real pregnancy rate is 60%, i.e. the number of actual groups included may be 19. During the period, the vaginal embolus may be missed and the pregnancy may be inaccurate. The number of weight measurements is mainly the initial weight measurements at the beginning of the experiment.
Weight gain group for ten days of pregnancy: the number of qualified experimental mice brought into E9.5d is determined by measuring the body weight after closing the cage for ten days. The experiment was performed in a cage on day 1 and in a cage on day 2, and the body weight of each mouse was recorded. Body weight was measured on day 11 of the experiment and included in the experimental group for pregnancy observation by identifying 16 mice with significant weight gain. The rest mice are in a male-female coop state. And dividing into cages on day 12. Body weight was measured on day 21 of the experiment and included in the experimental group for pregnancy observation by identifying 8 mice with significant weight gain. The rest mice are in a male-female coop state. The experiment was divided into cages on day 22. On day 30 of the experiment, 24 total may be included in the experimental group.
The invention group: the experiment is small in white, and the number of qualified experimental mice brought into E9.5d is determined by using the method. The experiment was performed in a cage on day 1 and in a cage on day 2, and the body weight of each mouse was recorded. The experiment was performed in 6 th day and in 7 th day. Body weight was measured on day 11 of the experiment and included in the experimental group for pregnancy observation by identifying 16 mice with significant weight gain. The rest mice are in a male-female coop state. And dividing into cages on day 12. Body weight was measured on day 16 of the experiment and included in the experimental group for pregnancy observation by identifying 8 mice with significant weight gain. The rest mice are in a male-female coop state. And (5) dividing the cages on the 17 th day. Body weight was measured on day 21 of the experiment and included in the experimental group for pregnancy observation by identifying 4 mice with significant weight gain. The rest mice are in a male-female coop state. And dividing the cage on the 22 nd day. Body weight was measured on day 26 of the experiment and included in the experimental group for pregnancy observation by identifying 2 mice with significant weight gain. The rest mice are in a male-female coop state. And 7, dividing the cage on the 27 th day. On day 30 of the experiment, a total of 30 may be included in the experimental group only.
Table 4: number of pregnant mice that may be included on a defined date within 30 days
Experimental arrangement schedule:
description of the experiment:
1. the mouse can not be weighed on the 6 th day of the experiment, if the mouse is pregnant, the weight of the mouse can be increased, but the weight of the mouse is greatly influenced by the age of the day, diet, drinking water, urination and the like, and the value of the fluctuation below 1g is mostly negligible. The observation of continuous weighing with emphasis can be taken, and the person with weight rising is pregnant. If the weight is repeated, the patient may suffer abortion or may not be pregnant.
2. The males of some experimental mice are easy to fight with the cages, so the ratio of the male and female cages is usually 2: 1. note that the time division cages, on the one hand, facilitate the differential recording of the pregnancy date, and, on the other hand, if the pregnant mice are in the same cage as other male mice within 24 hours of the initial pregnancy, may result in abortion in the pregnant mice due to the Bruce effect.
3. The application range of the experiment is wide, and the mice comprise c57BL, 129/SvEvBrd and the like.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited by the foregoing examples, which are provided to illustrate the principles of the invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention, which is also intended to be covered by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (2)
1. A feeding method capable of timely and accurately detecting pregnancy of an experimental mouse is characterized by comprising the following steps:
1) c57BL/6JA male and female mice 8-16 weeks old are bred in cages for 7 days in an adaptive manner;
2) breeding male and female mice in a cage for 1 day;
3) breeding the male and female mice in cages for 4 days; marking the female mice respectively on the day 1 of cage breeding, and recording the weight of each female mouse respectively;
4) breeding male and female mice in a cage randomly for 1 day;
5) breeding the male and female mice in cages for 4 days; determining female mice with the weight increased by 3.48 +/-0.47 g as pregnant mice on the day 4 of cage feeding, and separating and introducing the pregnant mice into a pregnancy period experiment;
6) the operation is cycled according to the steps 4) and 5).
2. The feeding method according to claim 1, wherein the male and female mice are randomly housed in a ratio of 2: 1 when they are housed in a cage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911134043.8A CN112088833A (en) | 2019-11-19 | 2019-11-19 | Feeding method capable of timely and accurately detecting pregnancy of experimental mice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911134043.8A CN112088833A (en) | 2019-11-19 | 2019-11-19 | Feeding method capable of timely and accurately detecting pregnancy of experimental mice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112088833A true CN112088833A (en) | 2020-12-18 |
Family
ID=73749210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911134043.8A Pending CN112088833A (en) | 2019-11-19 | 2019-11-19 | Feeding method capable of timely and accurately detecting pregnancy of experimental mice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112088833A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130017500A (en) * | 2011-08-11 | 2013-02-20 | 의료법인 성광의료재단 | Process for preparing mice having enhanced reproductive performance using fenarimol and pharmaceutical composition comprising the same |
| CN106070009A (en) * | 2016-06-16 | 2016-11-09 | 斯贝福（北京）生物技术有限公司 | A kind of SD rat production method |
| CN110447599A (en) * | 2019-08-30 | 2019-11-15 | 广西医科大学 | A kind of hero mouse reproductive capacity integrated evaluating method |
-
2019
- 2019-11-19 CN CN201911134043.8A patent/CN112088833A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130017500A (en) * | 2011-08-11 | 2013-02-20 | 의료법인 성광의료재단 | Process for preparing mice having enhanced reproductive performance using fenarimol and pharmaceutical composition comprising the same |
| CN106070009A (en) * | 2016-06-16 | 2016-11-09 | 斯贝福（北京）生物技术有限公司 | A kind of SD rat production method |
| CN110447599A (en) * | 2019-08-30 | 2019-11-15 | 广西医科大学 | A kind of hero mouse reproductive capacity integrated evaluating method |
Non-Patent Citations (2)
| Title |
|---|
| 胡雷: "《药物非临床研究与临床试验质量管理实物全书 上》", 30 September 2003, 当代中国音像出版社 * |
| 闫星泉 等: "三种检测BALB/c小鼠受孕方法的比较研究", 《实验动物科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kustritz | The value of canine semen evaluation for practitioners | |
| Chalkias et al. | Genetic analysis of functional and non-functional teats in a population of Yorkshire pigs | |
| Kabiraj et al. | Testicular biometry and its relationship with body weight and semen output of black Bengal bucks in Bangladesh | |
| Van Metre et al. | Factors associated with failure in breeding soundness examination of Western USA rams | |
| Ochiogu et al. | A new and simple method of confirmatory detection of mating in albino rats (Rattus norvegicus) | |
| Lane et al. | The influence of cow and management factors on reproductive performance of Irish seasonal calving dairy cows | |
| Carnevale et al. | Equine aging and the oocyte: a potential model for reproductive aging in women | |
| Setiaji et al. | Growth analysis in rabbit using Gompertz non-linear model | |
| CN112088833A (en) | Feeding method capable of timely and accurately detecting pregnancy of experimental mice | |
| Lavara et al. | Estimation of genetic parameters for semen quality traits and growth rate in a paternal rabbit line | |
| Tsiligianni et al. | The use of cytological examination of vaginal smears (CEVS) in the selection of rabbits for superovulation | |
| CN110447599B (en) | Comprehensive evaluation method for fertility of male mice | |
| Larson et al. | Evaluating information obtained from diagnosis of pregnancy status of beef herds | |
| Kim et al. | Duration of gestation in pregnant dogs carrying cloned fetuses | |
| Singleton et al. | Variation among boars in semen characteristics and fertility | |
| Siddiqua et al. | Evaluation of semen quality of Black Bengal Goat in Bangladesh | |
| CN208283126U (en) | Jenny ass urine collector | |
| Stolzenberg et al. | Studies with timed-pregnant squirrel monkeys (Saimiri sciureus) | |
| Sharma et al. | Relationship of Energy Reserves with Post-partum Sub-clinical Endometritis and its Impact on Reproductive Performance of Dairy Cows. | |
| DAŞKIN et al. | Artificial insemination in a cat: Report of first successful performance resulted with parturition in Turkey | |
| Makhafola et al. | Relationship between phenotypic and sperm traits of South African indigenous cockerels | |
| Tombuku et al. | Blood biochemical profile of bali cattle with repeated breeding condition | |
| Skliarov et al. | Determining the optimal time of insemination of goats using a thermal imager | |
| CN109971821A (en) | A kind of safety detecting method of supplementary reproduction liquid reagent | |
| Itodo et al. | Determination of the ovulation time in the laboratory rats (rattus norvegicus) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201218 |