CN106069896A - A kind of ecological breeding method improving larvae survival rate - Google Patents

A kind of ecological breeding method improving larvae survival rate Download PDF

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Publication number
CN106069896A
CN106069896A CN201610422046.1A CN201610422046A CN106069896A CN 106069896 A CN106069896 A CN 106069896A CN 201610422046 A CN201610422046 A CN 201610422046A CN 106069896 A CN106069896 A CN 106069896A
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CN
China
Prior art keywords
survival rate
ovum
parent population
breeding method
ecological breeding
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CN201610422046.1A
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Chinese (zh)
Inventor
张志勇
张志伟
许津
刘海林
沈德华
张曹进
吴国均
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Institute Of Oceanology & Marine Fisheries Jiangsu
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Institute Of Oceanology & Marine Fisheries Jiangsu
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Priority to CN201610422046.1A priority Critical patent/CN106069896A/en
Publication of CN106069896A publication Critical patent/CN106069896A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Behavior & Ethology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of ecological breeding method improving larvae survival rate, comprising: female parent population and male parent population are placed with the culturing pool of outer discharge outlet according to the ratio of 2:1;Mating period, embryonated egg collected by discharge outlet mesh bag outside;Hydrometer method is utilized to separate the ovum being fertilized with unfertilized ovum;Net cage micro-inflation hatching that the good ovum being fertilized is put in hatchery;Put into embryonated egg intact for growth after hatch in nursery pond;After prelarva hatches, adding chlorella algae solution at breeding water body, density is maintained at 20 × 104~30 × 104Between ind/ml;During prelarva opening, adding wheel animalcule, density domination is between 6~8ind/ml.The method of the present invention can be greatly improved the survival rate of prelarva, reduce quantity of exchanged water, is a kind of efficient ecological breeding method.

Description

A kind of ecological breeding method improving larvae survival rate
Technical field
The invention belongs to aquatic product sprout Cultivating techniques field, particularly to a kind of ecological breeding side improving larvae survival rate Method.
Background technology
China is the maximum sea-farming big country in the whole world, and within 2013, sea-farming yield reaches 1739.25 ten thousand t, accounts for generation More than the 70% of boundary's sea-farming total output, is the country that unique cultured output exceedes fishery output in the world.
Marine fish culture industry quickly grows, and cultured area and scale are increasing, and the species of cultivation and quantity are increasingly Many, sea-farming occupies critical positions.
Seed rearing is the vital link of marine fish culture, and the breakthrough of artificial breeding technology can solve seedling The problem planting short supply, enables aquaculture health, stable, sustainable development.The seedling-raising technique of low consumption high yield can be significantly Improve weed survival rate, reduction disposal of pollutants, increase production and improve productivity, improve the supply of seed more, at marine fish culture In, play very important effect.
Content of the invention
The technical problem to be solved is to provide a kind of ecological breeding method improving larvae survival rate, the method By building microecosystem, rate of water exchange can be reduced, improve larvae survival rate.
A kind of ecological breeding method improving larvae survival rate of the present invention, comprising:
(1) female parent population and male parent population are placed with the culturing pool of outer discharge outlet according to the ratio of 2:1;
(2) mating period, utilizing flowing water to go out chalaza, embryonated egg collected by discharge outlet mesh bag outside;
(3) hydrometer method is utilized to separate the ovum being fertilized with unfertilized ovum;
(4) the net cage micro-inflation hatching good ovum (growing intact) being fertilized being put in hatchery;
(5) put into embryonated egg intact for growth after hatch in nursery pond;
(6) after prelarva hatches, adding chlorella algae solution at breeding water body, density is maintained at 20 × 104~30 × 104ind/ Between ml;
(7) during prelarva opening, adding wheel animalcule, density domination is between 6~8ind/ml.
In described step (1) female parent population and great ambition parent population body surface is complete, body colour is gorgeous, vigor good, healthy lossless.
After in described step (1), female parent population and male parent population put into culturing pool, density is 6 tails/m3
In described step (1), the average weight of female parent population and male parent population is 1kg.
In described step (2), mesh bag mesh is 40 mesh;The bolting silk of 40 mesh is done and becomes band, be fixed together with elbow, use Collect ovum in discharge outlet outside.
In described step (3), ovum is buoyant egg, is separated by hydrometer method: the ovum of collection is put into the sea of salinity 36 ‰ In water, growing intact embryonated egg and floating, rudimentary ovum and impurity will sink;Before prelarva emersion, reuse proportion Method separates hatching with unhatched embryonated egg, and collects to hatch and grows intact embryonated egg.
Container used by described separation embryonated egg is the beneath transparent utensil with valve, the transparent body being easy to observe embryonated egg Long-pending, rudimentary embryonated egg and impurity can be discharged by beneath band valve easily.
The cloth ovum density that in described step (5), embryonated egg is put in nursery pond is 120ml/m2
In described step (5), the initial depth of water of nursery pond is 50cm.
The present invention can be greatly improved the survival rate of prelarva, reduce quantity of exchanged water, is a kind of efficient ecological breeding method.
Beneficial effect
In the present invention, chlorella carries out self-reproduction by photosynthesis in water, consumes CO2While release O2, supply Wheel animalcule and prelarva respiratory metabolism, provide sufficient bait for wheel animalcule simultaneously;Wheel animalcule by the chlorella that ingests, keeps one in water body Fixed density and fresh and alive degree, provide open-mouthed bait for prelarva.The present invention uses chlorella, wheel animalcule, prelarva, structure in seedling water Build miniature biochemical analyzer, while reducing quantity of exchanged water, substantially increase larvae survival rate, seawater fish seed rearing is played non- Often important effect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention Rather than restriction the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, people in the art The present invention can be made various changes or modifications by member, and these equivalent form of values fall within the application appended claims equally and limited Scope.
Embodiment 1
1st, the selection of parent population and cultivation: gather that body surface color is normal, vigor good, black porgy raun healthy, anosis, sexually matured With milter as parent, sex ration is 2:1, and cultivation density is 6 tails/m3, the average weight of parent population is 1kg;
2nd, the annual early or mid April in Jiangsu Province, when natural temperature reaches 16 DEG C, black porgy i.e. enters mating period, logical Cross flowing water embryonated egg is taken out of, utilize the mesh bag of 40 mesh to collect embryonated egg in discharge outlet;The bolting silk of 40 mesh is done and becomes band, and curved Head is fixed together, and discharge outlet collects ovum outside.
3rd, the embryonated egg of collection is rinsed well, go the removal of impurity, allocate salinity with sea-water extract, make salinity reach 36 ‰, fall Enter in the valvular transparent vessel of bottom belt, then embryonated egg is poured into wherein;
4th, owing to black porgy ovum is buoyant egg, well-developed embryonated egg all swims in water body upper strata, by opening bottom valve Unfertilized and hypogenetic ovum is drained by door, and add up the good egg volume being fertilized is 3L simultaneously;
5th, adding nature seawater in hatchery, net cage puts into it, puts in net cage by the embryonated egg of collection, micro-inflate into Row hatching.
6th, before prelarva emersion, reuse hydrometer method and separate hatching with unhatched embryonated egg, collect and incubate The embryonated egg volume changed is 2.4L, and calculating and obtaining incubation rate is 80%;
7th, it is 20m by area2Nursery pond enters water level to 50cm, adds density to be 500 × 104The bead algae solution of ind/ml 0.5m3, step into well-developed for 2.4L embryonated egg in pond;
8th, in salinity 23 ‰, under the conditions of water temperature 19.6 DEG C, newly hatched larvae 3 days just opening, now, by the density of 8ind/ml Add wheel animalcule as the open-mouthed bait of prelarva;
9th, the density of chlorella and wheel animalcule is checked every day so that it is be kept at 20 × 104~30 × 104Ind/ml and 6~ The density of 8ind/ml, keeps not changing for 20 days water, and statistics of drawing a design at random postlarva quantity, being calculated as motility rate is 86%, than traditional nursery The survival rate of method 58% significantly improves.
Embodiment 2
1st, the selection of parent population and cultivation: gather that body surface color is normal, vigor good, large yellow croaker healthy, anosis, sexually matured is female Fish and milter are as parent, and sex ration is 2:1, and cultivation density is 6 tails/m3, the average weight of parent population is 1kg;
2nd, in southern Jiangsu Province, large yellow croaker parent survives the winter to heat up at indoors artificial and carries out, and takes to strengthen nutrition, control The measures such as illumination promote parent population gonadal maturation, annual late March to early April, cultivation water temperature rise to 18 DEG C, large yellow croaker I.e. enter mating period, by flowing water, embryonated egg is taken out of, utilize the mesh bag of 40 mesh to collect embryonated egg in discharge outlet;By 40 purposes Bolting silk does and becomes band, and is fixed together with elbow, and discharge outlet collects ovum outside.
3rd, the embryonated egg of collection is rinsed well, go the removal of impurity, allocate salinity with sea-water extract, make salinity reach 36 ‰, fall Enter in the valvular transparent vessel of bottom belt, then embryonated egg is poured into wherein;
4th, owing to rheum officinale fish-egg is buoyant egg, well-developed embryonated egg all swims in water body upper strata, bottom opening Unfertilized and hypogenetic ovum is drained by valve, and add up the good egg volume being fertilized is 2.8L simultaneously;
5th, adding nature seawater in hatchery, net cage puts into it, puts in net cage by the embryonated egg of collection, micro-inflate into Row hatching.
6th, before prelarva emersion, reuse hydrometer method and separate hatching with unhatched embryonated egg, collect and incubate The embryonated egg volume changed is 2.4L, and calculating and obtaining incubation rate is 85.7%;
7th, it is 20m by area2Nursery pond enters water level to 50cm, adds density to be 500 × 104The bead algae solution of ind/ml 0.5m3, step into well-developed for 2.4L embryonated egg in pond;
8th, in salinity 23 ‰, under the conditions of water temperature 20.8 DEG C, newly hatched larvae 4 days just opening, now, by the density of 8ind/ml Add wheel animalcule as the open-mouthed bait of prelarva;
9th, the density of chlorella and wheel animalcule is checked every day so that it is be kept at 20 × 104~30 × 104Ind/ml and 6~ The density of 8ind/ml, keeps not changing for 20 days water, and statistics of drawing a design at random postlarva quantity, being calculated as motility rate is 89%, than traditional nursery The survival rate of method 76% significantly improves.

Claims (8)

1. the ecological breeding method improving larvae survival rate, comprising:
(1) female parent population and male parent population are placed with the culturing pool of outer discharge outlet according to the ratio of 2:1;
(2) mating period, embryonated egg collected by discharge outlet mesh bag outside;
(3) hydrometer method is utilized to separate the ovum being fertilized with unfertilized ovum;
(4) the net cage micro-inflation hatching good ovum being fertilized being put in hatchery;
(5) put into embryonated egg intact for growth after hatch in nursery pond;
(6) after prelarva hatches, adding chlorella algae solution at breeding water body, density is maintained at 20 × 104~30 × 104Ind/ml it Between;
(7) during prelarva opening, adding wheel animalcule, density domination is between 6~8ind/ml.
2. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (1) in, female parent population and great ambition parent population body surface are complete, healthy lossless.
3. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (1) after in, female parent population and male parent population put into culturing pool, density is 6 tails/m3
4. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (1) in, the average weight of female parent population and male parent population is 1kg.
5. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (2) in, mesh bag mesh is 40 mesh.
6. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (3) in, ovum is buoyant egg, is separated by hydrometer method: puts into the ovum of collection in the seawater of salinity 36 ‰, grows intact Embryonated egg is floated, and rudimentary ovum and impurity will sink;Before prelarva emersion, reusing hydrometer method will hatch and not incubate The embryonated egg changed separates, and collects the intact embryonated egg of the growth hatched.
7. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (5) the cloth ovum density that in, embryonated egg is put in nursery pond is 120ml/m2
8. a kind of ecological breeding method improving larvae survival rate according to claim 1, it is characterised in that described step (5) in, the initial depth of water of nursery pond is 50cm.
CN201610422046.1A 2016-06-14 2016-06-14 A kind of ecological breeding method improving larvae survival rate Pending CN106069896A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3683854A (en) * 1970-09-17 1972-08-15 Oceanography Mariculture Ind Apparatus for separating viable fish eggs from non-fertilized eggs
CN101897308A (en) * 2010-07-29 2010-12-01 中国水产科学研究院黄海水产研究所 Equipment and method for collecting industrially cultivated pelagic eggs
CN102388823A (en) * 2011-08-15 2012-03-28 中国水产科学研究院淡水渔业研究中心 Automatic collection device for semi-floating fish eggs
CN103478022A (en) * 2013-08-07 2014-01-01 泰州市秋雪湖渔业有限公司 Method for promoting nature spawning of american shad by using artificial culture ecological factor
CN104719204A (en) * 2015-03-19 2015-06-24 中国水产科学研究院东海水产研究所 Pampus argenteus fertilized egg selection device and application method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3683854A (en) * 1970-09-17 1972-08-15 Oceanography Mariculture Ind Apparatus for separating viable fish eggs from non-fertilized eggs
CN101897308A (en) * 2010-07-29 2010-12-01 中国水产科学研究院黄海水产研究所 Equipment and method for collecting industrially cultivated pelagic eggs
CN102388823A (en) * 2011-08-15 2012-03-28 中国水产科学研究院淡水渔业研究中心 Automatic collection device for semi-floating fish eggs
CN103478022A (en) * 2013-08-07 2014-01-01 泰州市秋雪湖渔业有限公司 Method for promoting nature spawning of american shad by using artificial culture ecological factor
CN104719204A (en) * 2015-03-19 2015-06-24 中国水产科学研究院东海水产研究所 Pampus argenteus fertilized egg selection device and application method thereof

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Title
柳学周等: "《半滑舌鳎繁育理论与养殖技术》", 31 May 2014, 中国农业出版社 *
江世贵等: "《中海近海鲷科鱼类种质资源及其利用》", 31 May 2012, 海洋出版社 *

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