CN106069187A - A kind of synthetic method of Phlebopus portentosus bacterium chamber insect gall - Google Patents
A kind of synthetic method of Phlebopus portentosus bacterium chamber insect gall Download PDFInfo
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- CN106069187A CN106069187A CN201610431882.6A CN201610431882A CN106069187A CN 106069187 A CN106069187 A CN 106069187A CN 201610431882 A CN201610431882 A CN 201610431882A CN 106069187 A CN106069187 A CN 106069187A
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- phlebopus portentosus
- insect gall
- bacterium chamber
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention relates to mushroom bionic cultivation field, in particular to the synthetic method of a kind of Phlebopus portentosus bacterium chamber insect gall.The synthetic method of this bacterium chamber insect gall, comprises the following steps: 1) Phlebopus portentosus solid original seed, Yunnan Prov. Inst. of Tropical Crops Science's plant protection provide with microbe research center bolete problem；2) picking mealybug inside the insect gall of bacterium chamber from the host's tree root of field, raises expanding propagation with Fructus Cucurbitae moschatae；3) aseptic seedling of host tree is cultivated；4) when aseptic seedling grows side root, at its root system inoculation Phlebopus portentosus solid spawn and mealybug, keeping substrate to moisten, maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains Phlebopus portentosus bacterium chamber insect gall.The synthetic method that the present invention provides can effectively set up the symbiosis of Phlebopus portentosus and host tree, it is possible to successful synthesis bacterium chamber insect gall, and bacterium chamber infection rate is up to 83% 90%.
Description
Technical field
The present invention relates to mushroom bionic cultivation field, in particular to a kind of Phlebopus portentosus bacterium chamber insect gall
Synthetic method.
Background technology
Phlebopus portentosus Phlebopus portentosus (Berk.&Broome) Boedijn, has another name called huge Hepar Bovis seu Bubali
Bacterium, popular name " Boletus aereus ", belong to Boletales little Boletaceae net arteries and veins handle Boletus.It is distributed in Sri Lanka, Vietnam, print
Degree Nicaea, Thailand, Brazil, Mexico, Australia, area, the domestic Yunnan that is mainly distributed on, Guangxi and the Hainan such as New Zealand
Also there is distribution.Its sporophore is very large, and meat is plump, and handle is sturdy, nutritious, and rich in proteins and potassium, calcium, magnesium, ferrum, zinc etc. are many
Planting the mineral element of needed by human body, its price is several times of common edible fungi, Shi Jibei, has the highest economic worth.
The Phlebopus portentosus tender sporophore meat of children is fine and smooth, tasty and deep liked by consumers in general, is old
Delicious food on common people's dining table.The Phlebopus portentosus supplied in the market is based on wild, the most in great demand, along with greatly
Measuring unordered business to gather, and the change of natural ecological environment, resource is petered out, and supply falls short of demand.Bionic cultivation is to increase
Resource, preserve the ecological environment, maintain the important directions of sustainable development.And bolete bacterium chamber insect gall is that dun net handle Hepar Bovis seu Bubali is bionical
The first step of cultivation, but it is difficult to synthetic bolete bacterium chamber insect gall at present, or the infection rate of bacterium chamber insect gall during synthetic
The lowest.
Summary of the invention
Present invention aim at solving above-mentioned prior art deficiency problem, it is provided that a kind of Phlebopus portentosus bacterium chamber insect gall
Synthetic method.Its synthetic method can effectively set up the symbiosis of Phlebopus portentosus and host tree, it is possible to success
Synthesis Phlebopus portentosus bacterium chamber insect gall, the infection rate of insect gall is 83%-90%.
The synthetic method of Phlebopus portentosus bacterium chamber of the present invention insect gall is to be completed by following concrete steps:
(1) the solid original seed of Phlebopus portentosus is obtained；
(2) picking mealybug inside the bacterium chamber insect gall from the host's tree root of field, raises expanding propagation with Fructus Cucurbitae moschatae；
(3) aseptic seedling of host tree is cultivated；
(4) when aseptic seedling grows side root, at its root system inoculation Phlebopus portentosus solid original seed and mealybug, base is kept
Matter moistens, and maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains Phlebopus portentosus bacterium chamber insect gall.
Mealybug in step (2) is ocean buttocks stricture of vagina mealybug (Planococcus minor), and selecting coffee tree is host tree
Kind；Use picking inside the bacterium chamber insect gall from host's tree coffee tree root, take back indoor microscopy and be followed by planting raising expansion on Fructus Cucurbitae moschatae
Numerous, Fructus Cucurbitae moschatae, with the alcohol disinfecting surface of 75%, can connect mealybug after drying；
The aseptic seedling of step (3) described cultivation host tree is to cultivate green coffee seeds to sprouting bud point, is transplanted into aseptic seedling
Culture medium is cultivated, after cultivation, obtains aseptic seedling；
Phlebopus portentosus solid original seed in described step (1), by Yunnan Prov. Inst. of Tropical Crops Science plant
Protection provides with microbe research center bolete problem, and its bacterium numbering: 13013, baterial cultivation chamber incubation time is 40 60d.
In step (2), the cultivation temperature in ocean buttocks stricture of vagina mealybug (Planococcus minor) is 25 DEG C ± 2 DEG C, Fructus Cucurbitae moschatae
Mealybug, with the alcohol disinfecting of 75%, can be raised in surface after drying.
In step (3), Aseptic seedling culture base includes following component, by volume: the peat composed of rotten mosses 30 50 parts, Vermiculitum 20 40
Part, cattle manure 10 30 parts, perlite 40 60 parts, vegetable garden soil 60 80 parts.
In step (3), the pH value 4.0 6.0 of Aseptic seedling culture base.
In step (4), the Phlebopus portentosus solid spawn of inoculation breaks into 3cm3–5cm3The block of size, every strain tree bacterium
The inoculum concentration planted is 50g 100g.
In step (4), the inoculum concentration in ocean buttocks stricture of vagina mealybug (Planococcus minor) is 50 100 heads/plant.
In step (4), soil humidity is maintained at 40% 60%, and maintaining temperature is 28 DEG C ± 2 DEG C.
Before inoculation Phlebopus portentosus solid spawn and ocean buttocks stricture of vagina mealybug, substrate to be carried out 121 DEG C of sterilizings 1
Hour sterilization treatment, can inoculate after cooling.
The present invention is by single culture Phlebopus portentosus solid original seed, ocean buttocks stricture of vagina mealybug and single culture host tree
Aseptic seedling, and by selecting to access Phlebopus portentosus solid original seed and ocean buttocks stricture of vagina powder when aseptic seedling grows side root
A red-spotted lizard, keeps substrate to moisten, and maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains Phlebopus portentosus bacterium chamber insect gall.
Synthetic method of the present invention can effectively set up the symbiosis of Phlebopus portentosus and host tree, it is possible to success
Synthesis Phlebopus portentosus bacterium chamber insect gall, the infection rate of bacterium chamber insect gall is 83%-90%.
Detailed description of the invention:
The synthetic method of Phlebopus portentosus bacterium chamber of the present invention insect gall, comprises the following steps:
(1) Phlebopus portentosus solid original seed, by Yunnan Prov. Inst. of Tropical Crops Science's plant protection and microorganism
Research center bolete problem provides；
(2) ocean buttocks stricture of vagina mealybug (Planococcus minor), picking inside the bacterium chamber insect gall from coffee tree root, use
Expanding propagation raised by Fructus Cucurbitae moschatae.
(3) green coffee seeds is cultivated to sprouting bud point, be transplanted in Aseptic seedling culture base cultivation and obtain aseptic seedling；
(4) when aseptic seedling grows side root, at its root system inoculation Phlebopus portentosus solid original seed and mealybug, base is kept
Matter moistens, and maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains Phlebopus portentosus bacterium chamber insect gall.
The solid original seed of the Phlebopus portentosus that the present invention selects, is protected by Yunnan Prov. Inst. of Tropical Crops Science plant
Protecting and provide with microbe research center bolete problem, its strain quality is guaranteed, and the probability of synthesis bacterium chamber insect gall is high.
Ocean buttocks stricture of vagina mealybug (Planococcus minor) that the present invention selects are in the bacterium chamber insect gall from coffee tree root
Face picking, takes back indoor microscopy and is followed by planting on Fructus Cucurbitae moschatae, and Fructus Cucurbitae moschatae, with the alcohol disinfecting surface of 75%, can connect mealybug after drying.
The present invention selects coffee tree to be host seeds, by single culture Phlebopus portentosus solid original seed, ocean buttocks
Stricture of vagina mealybug and the aseptic seedling of single culture host tree, and access dun net handle Hepar Bovis seu Bubali again when aseptic seedling grows side root by selection
Bacterium solid original seed and ocean buttocks stricture of vagina mealybug, keep substrate to moisten, and maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains dun net handle cattle
Liver bacterium bacterium chamber insect gall.
The bacterium numbering of the Phlebopus portentosus solid original seed selected in step (1) is 13013, baterial cultivation chamber incubation time
For 40-60d, during inoculation, strain breaks into 3cm3-5cm3The block of size, inoculum concentration is 50g-100g/ strain tree.
During raising ocean buttocks stricture of vagina mealybug, by test, inventor show that ocean buttocks stricture of vagina mealybug raised by employing Fructus Cucurbitae moschatae
Temperature should control, at 25 DEG C ± 2 DEG C, to be all unfavorable for the breeding of mealybug higher or lower than this temperature range.
Aseptic seedling culture base of the present invention includes following component, by volume: the peat composed of rotten mosses 30 50 parts, Vermiculitum 20 40 parts, cattle manure
10 30 parts, perlite 40 60 parts, vegetable garden soil 60 80 parts.
Inventor is by finding in test, and Aseptic seedling culture base pH value is 4.0 6.0, and the mycelia of Phlebopus portentosus is raw
Long speed, and when pH value is less than 4.0 or is more than 6.0, the growth of mycelia is all suppressed, so needing before building-up process
The pH value adjusting Aseptic seedling culture base in time is 4.0 6.0.
In order to eliminate the impact on synthesis bacterium chamber insect gall of the miscellaneous bacteria in aseptic seedling substrate, solid at inoculation Phlebopus portentosus
Before body original seed and ocean buttocks stricture of vagina mealybug, aseptic seedling substrate to be carried out 121 DEG C of sterilizings sterilization treatment of 1 hour, cool down rear
Can inoculate.
Embodiment 1
(1) Phlebopus portentosus solid original seed is ground with microorganism by Yunnan Prov. Inst. of Tropical Crops Science's plant protection
Studying carefully center bolete problem to provide, bacterium numbering is: 13013, and baterial cultivation chamber incubation time is 40d, checks strain quality before inoculation,
Mycelia covers with bacterium bag, yellowish-brown, and dense, sturdy, uniformly, always, without drying shrinkage, the strain being close to bacterium bag is qualified bacterium to color and luster in growth
Kind；
(2) tile bottom tissue culture bottle a metafiltration paper, soaks with tap water, then fastens with wrapping paper, 121 DEG C of sterilizings
30min, cools down stand-by；
(3) selected coffee mature seed, rinses 30min with tap water, uses 3%H afterwards2O2Soak 5min, constantly shake simultaneously
Dynamic, then use aseptic water washing three times.Put in the tissue culture bottle of sterilizing, put into 4 mature seeds for every bottle, be placed in 27 DEG C ± 3 DEG C
Environment in dark culturing, check every day 2 times, reject pollution microbes seed, sprout a little to seed germination；
(4) ocean buttocks stricture of vagina mealybug (Planococcus minor).Picking inside bacterium chamber insect gall from coffee tree root, uses
Expanding propagation raised by Fructus Cucurbitae moschatae, and cultivation temperature is 25 DEG C ± 2 DEG C, and mealybug, with the alcohol disinfecting of 75%, can be raised in Fructus Cucurbitae moschatae surface after drying.
(5) configuration Aseptic seedling culture base is weighed in following ratio.By volume, each component of Aseptic seedling culture base: the peat composed of rotten mosses
30kg, Vermiculitum 20kg, cattle manure 10kg, perlite 40kg, vegetable garden soil 60kg；First the peat composed of rotten mosses, Vermiculitum, perlite are prewetted, add
Mix after vegetable garden soil, cattle manure, regulate pH value 4.0, at 121 DEG C of sterilizing 1h, stand-by after cooling；
(6) straight mouth vial, stone potassium permanganate are sterilized, and in straight mouth vial, 3-4 grain stone is put in bottom, then will
Culture medium loads in straight mouth vial, the seed sprouted is transplanted in vial, is placed in warmhouse booth, keeps substrate wet
Profit, cultivates aseptic seedling at a temperature of 28 DEG C ± 2 DEG C；
(7) when aseptic seedling grows side root, inoculating bolete solid original seed at its root system, strain amount is 50g/ strain, simultaneously
Accessing coccid, connect worm amount and be 50-100 head/plant, after inoculation, bottle wall black plastic cloth wraps up vial, dark in keeping bottle as far as possible
Optical condition, keeps substrate to moisten, cultivates 3 months in the environment that temperature is 28 DEG C ± 2 DEG C；
In the present embodiment, the infection rate of bacterium chamber insect gall is 85.17%.
Embodiment 2
(1) Phlebopus portentosus solid original seed is numbered: 13013, and strain is by Yunnan Province's tropical crops scientific research
Institute's plant protection provides with microbe research center bolete problem, and baterial cultivation chamber incubation time is 50d, checks strain matter before inoculation
Measuring, mycelia covers with bacterium bag, yellowish-brown, and dense, sturdy, uniformly, color and luster always, without drying shrinkage, is close to the strain of bacterium bag for closing in growth
Lattice strain；
(2) tile bottom tissue culture bottle a metafiltration paper, soaks with tap water, then fastens with wrapping paper, 121 DEG C of sterilizings
30min, cools down stand-by；
(3) selected coffee mature seed, rinses 30min with tap water, uses 3%H afterwards2O2Soak 5min, constantly shake simultaneously
Dynamic, then use aseptic water washing three times.Put in the tissue culture bottle of sterilizing, put into 4 mature seeds for every bottle, be placed in 27 DEG C ± 3 DEG C
Environment in dark culturing, check every day 2 times, reject pollution microbes seed, sprout a little to seed germination；
(4) ocean buttocks stricture of vagina mealybug (Planococcus minor), picking inside the bacterium chamber insect gall from coffee tree root, use
Expanding propagation raised by Fructus Cucurbitae moschatae, and cultivation temperature is 25 DEG C ± 2 DEG C, and mealybug, with the alcohol disinfecting of 75%, can be raised in Fructus Cucurbitae moschatae surface after drying.
(5) in following ratio weigh configuration Aseptic seedling culture base, the by volume each component of Aseptic seedling culture base: the peat composed of rotten mosses
50kg, Vermiculitum 30kg, cattle manure 30kg, perlite 50kg, vegetable garden soil 70kg；The peat composed of rotten mosses, Vermiculitum, perlite are prewetted, adds dish
Garden mould, cattle manure mixing, regulate 5.0,121 DEG C of sterilizing 1h of pH value, stand-by after cooling；
(6) straight mouth vial, stone potassium permanganate are sterilized, and in straight mouth vial, 3-4 grain stone is put in bottom, then will
Culture medium loads in straight mouth vial, the seed sprouted is transplanted in vial, is placed in warmhouse booth, keeps substrate wet
Profit, cultivates aseptic seedling at a temperature of 28 DEG C ± 2 DEG C；
(7) when aseptic seedling grows side root, inoculating bolete solid original seed at its root system, strain amount is 50g/ strain, simultaneously
Accessing coccid, connect worm amount and be 50-100 head/plant, after inoculation, bottle wall black plastic cloth wraps up vial, dark in keeping bottle as far as possible
Optical condition, keeps substrate to moisten, cultivates 3 months in the environment that temperature is 28 DEG C ± 2 DEG C；
The infection rate of example 2 bacterium chamber insect gall is 89.37%.
Embodiment 3
(1) Phlebopus portentosus solid original seed is numbered: 13013, and baterial cultivation chamber incubation time is 60d, inspection before inoculation
Looking into strain quality, mycelia covers with bacterium bag, yellowish-brown, and dense, sturdy, uniformly, color and luster always, without drying shrinkage, is close to bacterium bag in growth
Strain is qualified strain；
(2) tile bottom tissue culture bottle a metafiltration paper, soaks with tap water, then fastens with wrapping paper, 121 DEG C of sterilizings
30min, cools down stand-by；
(3) selected coffee mature seed, rinses 30min with tap water, uses 3%H afterwards2O2Soak 5min, constantly shake simultaneously
Dynamic, then use aseptic water washing three times.Put in the tissue culture bottle of sterilizing, put into 4 seeds for every bottle, be placed in the ring of 27 DEG C ± 3 DEG C
Dark culturing in border, checks 2 every day, rejects the seed of pollution microbes, sprouts a little to seed germination；
(4) ocean buttocks stricture of vagina mealybug (Planococcus minor), picking inside the bacterium chamber insect gall from coffee tree root, use
Expanding propagation raised by Fructus Cucurbitae moschatae, and cultivation temperature is 25 DEG C ± 2 DEG C, and mealybug, with the alcohol disinfecting of 75%, can be raised in Fructus Cucurbitae moschatae surface after drying.
(5) configuration Aseptic seedling culture base is weighed in following ratio.By volume, each component of Aseptic seedling culture base: the peat composed of rotten mosses
40kg, Vermiculitum 40kg, cattle manure 20kg, perlite 60kg, vegetable garden soil 80kg；The peat composed of rotten mosses, Vermiculitum, perlite are prewetted, adds vegetable garden
Soil, cattle manure mixing, regulate 6.0,121 DEG C of sterilizing 1h of pH value, stand-by after cooling；
(6) straight mouth vial, stone potassium permanganate are sterilized, and in straight mouth vial, 3-4 grain stone is put in bottom, then will
Culture medium loads in straight mouth vial, the seed sprouted is transplanted in vial, is placed in warmhouse booth, keeps substrate wet
Profit, cultivates aseptic seedling at a temperature of 28 DEG C ± 2 DEG C；
(7) when aseptic seedling grows side root, inoculating bolete strain at its root system, strain amount is 50g/ strain, is concurrently accessed
Mealybug, connects worm amount and is 50-100 head/plant, and after inoculation, bottle wall black plastic cloth wraps up vial, keeps half-light bar in bottle as far as possible
Part, keeps substrate to moisten, cultivates 3 months in the environment that temperature is 28 DEG C ± 2 DEG C；
The infection rate of example 3 bacterium chamber insect gall is 83.26%.
Claims (10)
1. the synthetic method of a Phlebopus portentosus bacterium chamber insect gall, it is characterised in that step is as follows
(1) the solid original seed of Phlebopus portentosus is obtained；
(2) picking mealybug inside the insect gall of bacterium chamber from the host's tree root of field, raises expanding propagation with Fructus Cucurbitae moschatae；
(3) aseptic seedling of host tree is cultivated；
(4) when aseptic seedling grows side root, at its root system inoculation Phlebopus portentosus solid original seed and mealybug, keep substrate wet
Profit, maintaining temperature is 28 DEG C ± 2 DEG C, cultivates and obtains Phlebopus portentosus bacterium chamber insect gall.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that step (2)
Described in mealybug be ocean buttocks stricture of vagina mealybug (Planococcus minor), select coffee tree be host seeds；Use from host
Picking inside bacterium chamber insect gall on tree coffee tree root, takes back indoor microscopy and is followed by planting raising expanding propagation on Fructus Cucurbitae moschatae, and Fructus Cucurbitae moschatae is with 75%
Alcohol disinfecting surface, mealybug can be connect after drying；
The aseptic seedling of step (3) described cultivation host tree is to cultivate green coffee seeds to sprout a little to sprouting, is transplanted into aseptic seedling training
Support in base and cultivate, after cultivation, obtain aseptic seedling.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that described step
(1) the Phlebopus portentosus solid original seed in, by Yunnan Prov. Inst. of Tropical Crops Science's plant protection and microbe research
Center bolete problem provides, and its bacterium numbering: 13013, baterial cultivation chamber incubation time is 40-60d.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that in step
(2), in, the cultivation temperature in ocean buttocks stricture of vagina mealybug (Planococcus minor) is 25 DEG C ± 2 DEG C, Fructus Cucurbitae moschatae surface with 75% wine
Essence sterilization, can raise mealybug after drying.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that in step
(3) in, Aseptic seedling culture base includes following component, by volume: the peat composed of rotten mosses 30 50 parts, Vermiculitum 20 40 parts, cattle manure 10 30 parts,
Perlite 40 60 parts, vegetable garden soil 60 80 parts.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that in step
(3) in, the pH value 4.0 6.0 of Aseptic seedling culture base.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 3 insect gall, it is characterised in that in step
(4), in, the Phlebopus portentosus solid original seed of inoculation breaks into 3cm3–5cm3The block of size, the inoculum concentration of every strain tree strain is
50g–100g。
8. according to the synthetic method of the Phlebopus portentosus bacterium chamber insect gall described in claim 1 or 4, it is characterised in that in step
Suddenly, in (4), the inoculum concentration in ocean buttocks stricture of vagina mealybug (Planococcus minor) is 50 100 heads/plant.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that in step
(4) in, soil humidity is maintained at 40% 60%, and maintaining temperature is 28 DEG C ± 2 DEG C.
The synthetic method of Phlebopus portentosus bacterium chamber the most according to claim 1 insect gall, it is characterised in that in inoculation
Before Phlebopus portentosus solid original seed and ocean buttocks stricture of vagina mealybug, substrate is carried out 121 DEG C of sterilizings sterilization treatment of 1 hour,
Can inoculate after cooling.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107493906A (en) * | 2017-09-12 | 2017-12-22 | 广西平果县惠民蚕业科技有限责任公司 | A kind of method for improving fragrant plant thatch fruit yield |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204131A (en) * | 2007-12-10 | 2008-06-25 | 丽水市林业科学研究所 | Artificial cultivation method for Suillus Luteus |
| CN101669430A (en) * | 2009-09-24 | 2010-03-17 | 云南省热带作物科学研究所 | Semi-artificial culture method of phlebopus portentosus |
-
2016
- 2016-06-17 CN CN201610431882.6A patent/CN106069187A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204131A (en) * | 2007-12-10 | 2008-06-25 | 丽水市林业科学研究所 | Artificial cultivation method for Suillus Luteus |
| CN101669430A (en) * | 2009-09-24 | 2010-03-17 | 云南省热带作物科学研究所 | Semi-artificial culture method of phlebopus portentosus |
Non-Patent Citations (2)
| Title |
|---|
| CHUN-XIA ZHANG等: "Fungus-insect gall of Phlebopus portentosus", 《MYCOLOGIA》 * |
| 刘静等: "柚子树下暗褐网柄牛肝菌的仿生栽培研究", 《中国热带农业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107493906A (en) * | 2017-09-12 | 2017-12-22 | 广西平果县惠民蚕业科技有限责任公司 | A kind of method for improving fragrant plant thatch fruit yield |
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Application publication date: 20161109 |