CN105998135A - Novel application of honeysuckle flower liquid - Google Patents
Novel application of honeysuckle flower liquid Download PDFInfo
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- CN105998135A CN105998135A CN201610521055.6A CN201610521055A CN105998135A CN 105998135 A CN105998135 A CN 105998135A CN 201610521055 A CN201610521055 A CN 201610521055A CN 105998135 A CN105998135 A CN 105998135A
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- honeysuckle flower
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- ephedrine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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Abstract
The invention discloses honeysuckle flower liquid resistant to ephedrine cardiomyocyte injury. The honeysuckle flower liquid is raw liquid medicine decoction of 0.9-1.5 g/mL. Based on extensive studies of honeysuckle flower, it is found that the honeysuckle flower liquid can promote cell metabolism, improve the antioxidant capacity of histocyte, inhibit peroxidatic reaction of cell membrane lipids, maintain membrane integrity and permeability, reduce structural damage of tissue and reduce the activity of serum CK and CK-MB, also has antioxidant, anti-inflammatory and cardiac histiocyte protecting effects, and can effectively reduce or relieve the damage of ephedrine to myocardial cells. Novel application of the honeysuckle flower liquid is realized, and the novel application has certain reference meaning for development and utilization of the honeysuckle flower medicinal resource in future.
Description
Technical field
The present invention relates to technical field of Chinese medicines, be specifically related to the new use of a kind of Flos Lonicerae liquid anti-ephedrine myocardial cell injury
On the way.
Background technology
Ephedrine is the main component in the medicines such as treatment flu, anaphylaxis, bronchial asthma and nasal decongestion, with
Time, ephedrine is again sympathomimetic, as synthesis methamphetamine hydrochloride and the important source material of head-shaking pill, life-time service or improper use,
Easily cause central nervous system's toxic and side effects, produce serious drug dependence, body tissue structure is had pathologic damage, seriously
Time can cause death.Therefore, body is had no side effect and drug resistance by research Chinese herbal medicine by animal or the protective effect of human body, research and development
The Chinese medicine preparation of thing damage, the development for medical career is significant.
At present, the analeptic such as ephedrine abuse, to abuse phenomenon relatively universal, ephedrine improper use and the untoward reaction that causes
Event occurs repeatedly.Commonly use medicinal analeptic addiction or the withdrawal symptom of eliminating such as methadone, clonidine the most both at home and abroad, but
These medicines itself also exist certain additive and drug resistance problems, therefore choose that toxic and side effects is little, dependency is little and drug resistance
Property little Chinese medicine, significant for reducing or reduce the damage that body tissue organ causes by upper.At present
Also there is no the Chinese medicine of the anti-ephedrine myocardial cell injury of special efficacy.
Summary of the invention
It is an object of the invention to provide the new application of a kind of Flos Lonicerae liquid, thin to reach composite for resisting ephedrine damage, protective tissue
The purpose that born of the same parents, promotion histiocyte are repaired.
The honeysuckle flower water decocting liquid alleged by present invention purposes in anti-ephedrine myocardial cell injury is exactly Flos Lonicerae decocting
Liquid is as the application in treatment ephedrine myocardial cell injury, and the concentration of this honeysuckle flower water decocting liquid is containing crude drug amount 0.9 ~ 1.5g/
mL。
The present invention studies discovery, and the process for producing of Flos Lonicerae liquid is simple, it is easy to large-scale production；Flos Lonicerae resource is rich
Rich；Pollution-free, small investment, medicine is inexpensive, draws materials conveniently；Toxicological experiment finds no toxicity；Thin to ephedrine cardiac muscle
Cellular damage has significant protective effect.
Accompanying drawing explanation
Fig. 1 is the change of mouse core organizational structure；
Wherein: A figure is blank group 5d mouse heart rip cutting；
B ~ D figure is experiment contrast group 5d, 10d, 15d murine myocardium structure, intercalated disc connect loose, intercalated disc gap broadening (↑),
Nuclear shapes is irregular and swelling, part cardiac muscle fiber fracture (↑)；
E ~ G figure is experimental group 5d, 10d, 15d murine myocardium structure, and myocardial ultramicrostructure is substantially complete, and intercalated disc connects and compares
Closely, intercalated disc gap reduces, and the fracture of cardiac muscle fiber is obviously controlled.
Detailed description of the invention
Further illustrate the present invention in conjunction with the embodiments.
Prepared by medicinal liquid
According to Chinese medicine decoct preparation method, extracting honeysuckle 100g, clear water boils 30min, filters medicine juice, medicinal residues after soaking 1h
Add clear water again to decoct 30min and filter medicine juice, merge twice medicinal liquid, then with Rotary Evaporators this decoct concentrated make 0.9 ~
The crude drug Flos Lonicerae liquid of 1.5g/mL.
Flos Lonicerae liquid causes the protective effect of myocardial cell injury to ephedrine
Select the kunming mice 90 of raw 10d, be randomly divided into blank group, experiment contrast (ephedrine) group and experiment (Herba Ephedrae
Element+Flos Lonicerae) group.Experiment contrast group and experimental group use ascending-dose (2.0g/L, 4.0g/L, 6.0g/L) ephedrine solution even
Continuous (1 ~ 5d, 6 ~ 10d, 11 ~ 15d) lumbar injection 15d, per injection 0.2mL (twice daily), after injection ephedrine 1h, experiment
Organize gavage 0.2mL Flos Lonicerae liquid again, the normal saline of experiment contrast group gavage equivalent, blank group lumbar injection and gavage etc.
The normal saline of amount.When testing 5d, 10d and 15d, put to death each group of mice.With CK, CK-MB in colorimetric determination mice serum
Activity, cardiac muscular tissue's T-AOC, CAT activity and MDA content, observe myocardial tissue structure with paraffin imbedding and HE staining
Pathological change, experimental data SPSS13.0 carries out statistical analysis, and result represents with mean+SD (Mean ± SD), grinds
Study carefully Flos Lonicerae liquid and ephedrine is caused the impact of myocardial tissue damage.Experiment finds experiment contrast group mice serum CK, CK-MB activity
Raise (P＜ 0.05 orP＜ 0.01)；Cardiac muscular tissue's T-AOC, CAT activity reduces, and MDA raises；Myocardial histopathology damage is bright
Aobvious, cardiac muscle fiber fall into disarray, intercalated disc connects loose, and part cardiac muscle fiber is ruptured, and cell boundary obscures, membranolysis, carefully
Karyon is degraded, interstitial edema, cavity occurs, and cardiac muscle is the most downright bad.The reduction of experimental mice serum CK, CK-MB activity (P＜
0.05 orP＜ 0.01)；Cardiac muscular tissue T-AOC, CAT activity raises, and MDA reduces, when 10d, 15d significant difference or extremely notable (P
＜ 0.05 orP＜ 0.01).Mouse cardiac muscle damages relatively ephedrine group and has clear improvement, and cellularity is substantially complete, myocardial cell
Arrangement is basic recovers normal, and intercalated disc connects relatively tightr, and cell boundary is more visible, and cardiac muscle fiber fracture is effectively controlled.Knot
Fruit is shown in Table 1 ~ 2, Fig. 1.
In sum, Flos Lonicerae liquid can promote cell metabolism, can be effectively improved histiocytic antioxidant activity,
Anti-lipid peroxidation reacts, and maintains film integrality and permeability, alleviates cell membrane damage, suppress or neutralize the work of inflammatory factor
Property, can substantially reduce ephedrine causes damage mice serum CK, CK-MB activity and cardiac muscular tissue's MDA content (table 1,2), alleviates fiber crops
Myocardial tissue damage caused by flavin and apoptosis, protection cardiac muscle is from the damage (Fig. 1) of ephedrine.Meanwhile, Flos Lonicerae liquid
Also there is safety, without dependence, nontoxic effect.
Claims (2)
1. Flos Lonicerae liquid purposes in anti-ephedrine myocardial cell injury.
Purposes the most according to claim 1, it is characterised in that: containing the Flos Lonicerae liquid that crude drug amount is 0.9 ~ 1.5g/ml anti-
Purposes in ephedrine myocardial cell injury.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521055.6A CN105998135B (en) | 2016-07-05 | 2016-07-05 | New application of honeysuckle liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610521055.6A CN105998135B (en) | 2016-07-05 | 2016-07-05 | New application of honeysuckle liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105998135A true CN105998135A (en) | 2016-10-12 |
| CN105998135B CN105998135B (en) | 2020-11-03 |
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ID=57107135
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610521055.6A Active CN105998135B (en) | 2016-07-05 | 2016-07-05 | New application of honeysuckle liquid |
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| Country | Link |
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| CN (1) | CN105998135B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109419840A (en) * | 2017-08-22 | 2019-03-05 | 北京中医药大学 | The new application of honeysuckle |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600233A (en) * | 2012-04-10 | 2012-07-25 | 西北师范大学 | Traditional Chinese medicine composition for repairing ephedrine tissue damage and preparation process thereof |
| CN103222999A (en) * | 2012-01-31 | 2013-07-31 | 财团法人工业技术研究院 | Honeysuckle extract and preparation method and application thereof |
| CN103285063A (en) * | 2013-05-15 | 2013-09-11 | 孙玉平 | Preparation method of honeysuckle extract |
-
2016
- 2016-07-05 CN CN201610521055.6A patent/CN105998135B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222999A (en) * | 2012-01-31 | 2013-07-31 | 财团法人工业技术研究院 | Honeysuckle extract and preparation method and application thereof |
| CN102600233A (en) * | 2012-04-10 | 2012-07-25 | 西北师范大学 | Traditional Chinese medicine composition for repairing ephedrine tissue damage and preparation process thereof |
| CN103285063A (en) * | 2013-05-15 | 2013-09-11 | 孙玉平 | Preparation method of honeysuckle extract |
Non-Patent Citations (2)
| Title |
|---|
| 王锦锦 等: "中药小复方(液)对麻黄素致损伤中肾SOD、CAT活性及Bax蛋白与TGF-β1表达的影响", 《中国细胞生物学学报》 * |
| 陈晓麟 等: "金银花水提取液抗氧化作用研究", 《时珍国医国药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109419840A (en) * | 2017-08-22 | 2019-03-05 | 北京中医药大学 | The new application of honeysuckle |
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| Publication number | Publication date |
|---|---|
| CN105998135B (en) | 2020-11-03 |
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