CN103893620B

CN103893620B - Chinese medicinal composition for preventing and treating diabetes and preparation method thereof

Info

Publication number: CN103893620B
Application number: CN201410055138.1A
Authority: CN
Inventors: 李正朝
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-02-18
Filing date: 2014-02-18
Publication date: 2015-04-08
Anticipated expiration: 2034-02-18
Also published as: CN103893620A

Abstract

The invention discloses a Chinese medicinal composition for preventing and treating diabetes and a preparation method thereof. The Chinese medicinal composition consists of the following components in parts by weight: 10-20 parts of vervain, 1-2 parts of typhonium giganteum, 10-20 parts of barley root, 10-20 parts of coptis root, 10-20 parts of black fungus, 10-20 parts of white fungus, 10-20 parts of sticky rice, 10-20 parts of barley rice, 10-20 parts of buckwheat, 5-50 parts of honey and 50-100 parts of vinegar. Natural animal and plant medicinal materials are taken as raw material sources, and diabetics syndrome and nephrotic syndrome are treated by using the comprehensive function of various Chinese medicinal components. As proved by acute toxicity test and long-term toxicity test, the Chinese medicinal composition is nontoxic to human body and good in medicinal effect and has the effects of improving the hypercoagulable state, improving blood fat, reducing blood sugar, dissolving thrombi, dredging microcirculation and improving the immunity of organisms.

Description

Chinese medicine composition of a kind of prevention, treatment diabetes and preparation method thereof

Technical field

The invention belongs to the field of Chinese medicines, particularly relate to a kind of prevention, the Chinese medicine composition for the treatment of diabetes and preparation method thereof.

Background technology

The diabetics of current China is numerous, the metabolic disease of diabetes to be one group with hyperglycemia be feature.Hyperglycemia be then due to defect of insulin secretion or its biological agent impaired, or both have concurrently and cause.Long-standing hyperglycemia during diabetes, causes various tissue, particularly eye, kidney, heart, blood vessel, neural chronic lesion, dysfunction.Patient stands ailing torment for a long time, mostly takes Western medicine in the market, can only control, can not effect a radical cure, even cause other complication even renal failure because of long-term taking Western medicine.In order to the present invention proposes the concept that Chinese medicine effects a permanent cure, through long-term R & D, pharmacological evaluation and clinical verification, draw pure natural medical of the present invention.

In addition, while preventing to make patient, treating diabetes, physical function does not fail, and also preferably adds Lumbrukinase.Lumbrukinase is a kind of histone hydrolase obtained from extraction and isolation in Lumbricus body, its main component is plasmin and plasminogen activator, these two kinds of compositions are the former dual function with activating fibrinolysin by direct fibrin degradation, fibrinogen content and hematoblastic aggregation rate can be reduced, strengthen Fibrinolytic Activities, blood coagulation after correction cerebral infarction and fibrinolytic are extremely, to diseases such as treatment angina pectoris, acute cerebral infarction, hyperlipidemia, hypertension, alleviate patient's slight illness and all there is good curative effect.Lumbrukinase is current one of fibrinolytic medicine having most DEVELOPMENT PROSPECT, no matter intravenous injection or oral administration, and thrombolytic effect all clearly.

Lumbrukinase, along with the dissolving of thrombosis, softening, the microcirculatory improvement of blood vessel, reduces blood viscosity, abnormal hemorheology index is made to recover normal, Microcirculation of Nailfold is detected transferred to normally by abnormal, thus eliminate the unexpected hidden danger of generation cardiovascular and cerebrovascular vessel, reduce blood glucose simultaneously, the positive source comprehensive effect optimum due to active earthworm protein improves the multiple function of human body, improve the environment of body, facilitate the recovery of islet function, after taking this product, have certain blood sugar reducing function.

Lumbrukinase extracts from Pheretima, and Pheretima has another name called Lumbricus, and [3] Pheretima is one of important Chinese crude drug of China.Just Lumbricus is had in the 67 kinds of animal drugs recorded in Chinese materia medica monograph Shennong's Herbal the earliest.Be classified as low-grades at Shennong's Herbal, there is heat clearing away arresting convulsion, dredging collateral, relieving asthma, effect of diuresis.For diseases such as high heat, coma, infantile convulsion tic, arthralgia, dyspnea and cough due to lung-heat, oliguria edema, hypertension after normal parch.Main containing several amino acids.Because raw product fishy smell is too heavy, therefore be used as medicine generally need through parch.TAO Hong-Jing is called: " if take dry earthworm, must endure and consider to be worth doing ".Tradition is with Pheretima, and method for making is a lot, mainly contains medicine system, vinegar system, boils, processed with wine, the method such as oil system, Pulvis concha Mactrae parch, salt, makes its quality pine steep crisp, detoxification, correct stink and be convenient to fried taking.

Pheretima is except having better nutritivity and being worth, and it also has a lot of health care, can strengthen the premunition of human body, improves the health level of people.In recent ten years, Pheretima is carried out to the large quantity research of chemistry, pharmacology aspect both at home and abroad, find Pheretima not only containing abundant nutritional labeling, also containing multiple pharmacological component, there is many-sided pharmacological action, relate to each system of human body as cardiovascular system, blood system, immune system, central nervous system, respiratory system, digestive system etc.Especially, in cardiovascular system unifies blood system, Pheretima can prophylaxis of hypertension, myocardial infarction, arrhythmia, apoplexy thromboembolism well.Application

At present, the application of China's Chinese medicine has more and more obtained the accreditation of international community, its multiple medical material is evident in efficacy, medicine for blood sugar lowering is various especially, but really evident in efficacy in practical application, only have several, such as Herb Gynostemmae Pentaphylli, Semen Cassiae etc., and the application mentions obtaining Herba Verbenae, Herba Typhonii gigantei, apply then little.The applicant finds in the leaching process at Lumbrukinase in the production and application of reality, Lumbricus is fed in advance with Chinese medicine, a certain amount of blood-sugar lowering tcm drug of natural compound, the Lumbrukinase at the place of compound collecting has remarkable result in blood sugar lowering, in blood fat reducing and blood pressure lowering, have significant curative effect too, successful is strengthened.

Summary of the invention

For realizing object of the present invention, concrete scheme is as follows:

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition forms by following weight proportion: Herba Verbenae 10 ~ 20 parts, Herba Typhonii gigantei 1 ~ 2 part, Semen Coicis root 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Auricularia 10 ~ 20 parts, 10 ~ 20 parts, Tremella, 10 ~ 20 parts, Oryza glutinosa, seed of Job's tears 10 ~ 20 parts, 10 ~ 20 parts, Semen Fagopyri Esculenti, Mel 5 ~ 50 parts, vinegar 50 ~ 100 parts.

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition forms by following weight proportion: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts.

A Chinese medicine composition for prevention, treatment diabetes, described formula forms by following weight: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts;

Described compositions configuration step one, take i.e. Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti by said ratio, by super micron mill, make superfine powder to 100 order after pulverizing stand-by; Prepared micropowder is put into container, adds pure water and boil extraction 2 times, first time adds 15-20 times of pure water and soaks 1 hour, heated and boiled 1 hour, and cooling precipitation 1-1.5 hour, gets supernatant; Second time adds 5-8 times of pure water heated and boiled 20-40 minute, and cooling precipitation 1-1.5 hour, gets supernatant; Merge first time and secondary supernatant, filter, obtain filtrate for subsequent use;

Described seed of Job's tears are by the seed of Job's tears of dried water content at below 8wt%, are placed in parch pot in 120 ~ 150 DEG C of parches 20 ~ 55 minutes, until surperficial yellowish-brown, namely take out for subsequent use; Described Semen Fagopyri Esculenti is by the seed of Job's tears of dried water content at below 8wt%, is placed in parch pot in 120 ~ 150 DEG C of parches 20 ~ 55 minutes, until surperficial yellowish-brown, namely takes out for subsequent use; Described Herba Verbenae comprises and cleaning up and Herba Verbenae stem and leaf, flower and seed after drying;

Step 2, according to proportioning, take Mel 28 parts, vinegar 75 parts, with vinegar Mel evenly rushed and, obtain honey vinegar solution, for subsequent use;

Raw material mixing above-mentioned steps one, two obtained, then stirs evenly, namely concentrating under reduced pressure, sterilizing obtain described prevention, treatment diabetes at the 60 DEG C Celsius paste semi-finished product of Chinese medicine composition;

By the semi-finished product granule of described Chinese medicine composition, pill, tablet, thick soup or add in capsule and make portable capsule.

A Chinese medicine composition for prevention, treatment diabetes, described formula forms by following weight: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts; Supplementary element 5 ~ 10 parts, described supplementary element is Lumbrukinase.

A Chinese medicine composition for prevention, treatment diabetes, described formula forms by following weight: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts; Supplementary element 5 ~ 10 parts, described supplementary element is the mixture of Chinese crude drug Radix Tylophorae arenicolae, Chinese crude drug leaf of Cyclocarya paliurus Iljinskaja, Chinese crude drug Conocephalum conicum(L．) Dumortier．, and Mixing ratio by weight example is 1:1 ~ 2:1.

A Chinese medicine composition for prevention, treatment diabetes, described Lumbrukinase is prepared by following steps:

1) vermiculture: select a day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Pheretima pingi, Amythas dancatala, large flat No. two earthworms; The described Lumbricus selected shifts to an earlier date 30 days and starts to feed Chinese medicine composite powder, and described Chinese medicine composite powder is by weight ratio: Radix Glycyrrhizae Preparata 5 parts, Radix Achyranthis Bidentatae 3 parts, process Hirudo 2 parts, Herba Lycopi 2 parts, Fructus Schisandrae Chinensis 2 parts, Radix Ophiopogonis 1 part, Rhizoma Polygonati Odorati 2 parts, Bulbus Allii Macrostemonis 1 part, Fructus Lycii 1.5 parts, Radix Et Caulis Acanthopanacis Senticosi 1 part, Cordyceps 1 part, Radix Puerariae 1 part; Described Chinese medicine composite powder is crushed to through super micron mill the powder that 300 ~ 450 orders make;

2) Lumbricus cleaning: optional step 1) Lumbricus alive that cultivates, remove the Lumbricus that impurity and dead are rotten, Lumbricus is put into the water 10 ~ 15 minutes that pH value is 4.5 ~ 6, add 60 ~ 65g Sal simultaneously, the 1-2 hour of illumination simultaneously, impels Lumbricus get rid of dirt in body and clean Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, uses the warm water washing of 25 ~ 27 DEG C, wash away excessive moisture and the impurity of outer surface;

3) slurrying: slurrying: the ethanol sodium citrate buffer solution that the pH value adding Lumbricus volume 3-6 times is 5.0 ~ 5.3, concentration is 0.1mol/L or pH value are 5.0 ~ 5.3, concentration is the sodium phosphate buffer of 0.1mol/L, after above-mentioned Lumbricus being carried out cell breakage with pearl mill, colloidal mill or high pressure homogenizer, the sodium citrate adding above-mentioned parts by weight carries out fully stirring making homogenate.

4) homogenate step 3) obtained is by filtering or the centrifugal removing of sedimentation centrifuge thick slag wherein, and the homogenate after slagging-off, adopts 5000 turns of centrifuge per minute 16 ~ 20 minutes or 0.45 micron pore size membrane filtration process, obtain clear liquor;

5) saltout: in step 4) gained clear liquor, add ammonium sulfate by 35% saturated concentration, stir and obtain mixed liquor in after dissolving completely standing 50 minutes;

6) centrifugal ultrafiltration: through high speed centrifugation, slag thin in mixed liquor in step 5) is removed with vertical high speed centrifugal machine, obtained clear liquid, then by 50000dt membrane ultrafiltration, obtain ultrafiltrate; 7) chromatographic column is separated: adopt anion-exchange chromatography post or affinity column separating step 6) ultrafiltrate that obtains, with petroleum ether, 0.005 ~ 1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of Peak Activity part; 8) sterilize: by above-mentioned steps 7) chromatography effluent put into the sealed sterilised container of carrying pipe, in container, high pressure nitrogen gas is injected through conduit, machinery fully stirs pending clear liquor 5 ~ 8min simultaneously, release pressure is exported through conduit, after pressure reduces, nitrogen solubility reduces rapidly, the rapid loss of a large amount of nitrogen gas in container; Then filter removal of contamination, precipitate 10 hours and obtain supernatant; 9) by the lyophilization of step 8) gained supernatant, in subzero 28 ~ 35 degree of freezer dryers freezing 5 ~ 8 hours, then under the vacuum of 0.1mmHg, 25 ~ 30 DEG C are warming up to, dry 8 hours; Continue to be warming up to 35 ~ 39 DEG C, vacuum drying 5-6 hour, obtains powdery product.

Preferably, the Chinese medicine composition of described a kind of prevention, treatment diabetes, is characterized in that: it is used to the medicament prepared treatment or prevent diabetes.

Preferably, the Chinese medicine composition of described a kind of prevention, treatment diabetes, is characterized in that: it is used to prepare treatment or the food that prevents diabetes or health product.

Preferably, the Chinese medicine composition of described a kind of prevention, treatment diabetes, is characterized in that: it is used to prepare the medicament for the treatment of or the complication that prevents diabetes.

Preferably, the preparation method of the Chinese medicine composition of described a kind of prevention, treatment diabetes, is characterized in that step is as follows:

1) commercially available super micron mill is selected;

2) Herba Verbenae 15 parts of respective amount, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti is taken, by super micron mill, make superfine powder to 100 order after pulverizing stand-by; Prepared micropowder is put into container, adds pure water and boil extraction 2 times, first time adds 15-20 times of pure water and soaks 1 hour, heated and boiled 1 hour, and cooling precipitation 1-1.5 hour, gets supernatant; Second time adds 5-8 times of pure water heated and boiled 20-40 minute, and cooling precipitation 1-1.5 hour, gets supernatant; Merge first time and secondary supernatant, filter, obtain filtrate for subsequent use;

Described Chinese medicinal ingredients is through to be verified for many years, records and test gained, although their effect is different, but choosing of each Chinese medicine is not random and be, the applicant is according to the proportioning of each composition of test gained for many years, be not by raw material effect simple superposition of each composition, because the multiple interactivelies such as interpromoting relation in five elements, mutual reinforcement between, mutual restraint between two drugs or the mutual-detoxication may be there is between various raw material of Chinese medicine, and the applicant have selected above-mentioned raw materials accordingly according to this principle exactly, and the optimum proportioning determined between them, therefore, the application has significant meaning.

Beneficial effect:

The present invention selects natural animal and plant medical material to be raw material sources, utilize the comprehensive function of each natural constituents, prevention, treatment diabetic complication, through acute toxicology and long_term toxcity test, prove the effect of human non-toxic's evil, its drug effect is high, can improve high blood coagulation state, improve blood fat, reduce blood glucose, thrombus, dredging microcirculation, improve immunity of organisms simultaneously.

The granule that the present invention makes, composition properties and characteristics is pitchy, powdery, has bitterness, soluble in water.Show through safety toxicology test: LD50 is 20000mg/kg, and Toxicological evaluation is nontoxic level, has no side effect.

Drink through human experiment, every day, 1-2 bag, often wrapped 5g.Have obesity (with hyperlipidemia), hypertension, hyperglycemic symptoms patient in 150 people, everyone drinks 30-60 days, and drug withdrawal 30-60 days.

Good significant blood sugar lowering, lipid-lowering effect are achieved to 50 hyperglycemic patients; After experimenter drinks 30 days, TC on average declines 1.12mmol/L, HDL raises 0.80mmol/L, wherein obviously decline TC and the effect raising HDL, its effect is higher than vitamin E, the average rate of decrease playing its glucose is 12.5%, and the average rate of decrease of fructosamine is check its blood glucose value all to reach normal level after 26.3%, 30 days.Result of the test shows: the health product Be very effective that the present invention relates to.For for causing that suffering from a deficiency of the kidney of diabetes is quenched one's thirst, stagnant in disorder of emotion, stomach, intrinsic heat dryness-transformation etc. pathogenic because of, fall apart with YIN nourishing stagnant, soothing the liverly to promote the production of body fluid, regulate the flow of vital energy eliminating evil for aim, ask removing heat-phlegm, loose strongly fragrantly to quench one's thirst, the merit of dryness and dampness coordinating each other, good absorbing effect, evident in efficacy, through clinical verification, be applicable to alleviation and the treatment of diabetes and diabetic complication.

In addition, wherein obesity patient's (with hyperlipidemic conditions) 50 people, through inspection fasting plasma lipid, T-CHOL CT, triglyceride TG and high density lipoprotein HDL, carry out drinking front and back contrast test, carry out comparitive study again: test drinks 30 days, and experimenter TC, TG significantly decline, and HDL obviously rises, TC declines 32.1%, 43.2%, 55.4% respectively;

In addition, wherein 50 hypertension symptom persons, after drinking 30 days, obtain good blood pressure lowering effect simultaneously, and mean blood pressure declines 30%, blood pressure drops amplitude: systolic pressure decline 3.16kpa, diastolic pressure decline 2.60%kpa, and its hypotensive effect is remarkable;

Show through pharmacological testing: the present invention selects natural animal and plant medical material to be raw material sources, utilize the comprehensive function of each Chinese medicinal components, prevention, treatment diabetic syndrome, through acute toxicology and long_term toxcity test, prove the effect of human non-toxic's evil, its drug effect is high, can improve high blood coagulation state, improve blood fat, reduce blood glucose, thrombus, dredging microcirculation, improve immunity of organisms simultaneously.

Specific embodiment

Embodiment 1

Herba Verbenae HerbaVerbenae (English) EuropeanVerbenaHerb, another name Herba lespedezae pilosae, purple top Agrimony, wild catmint.

Herba Verbenae is originally the medicine of removing heat from blood removing blood stasis.Bottom abscess person, the pole of heat in blood, furthermore damp and hot, therefore blood stains are turbid forms skin ulcer, and have worm also.The cool pyrolysis of blood, the broken and row of dirty person, waste and be not recovered.CHEN Zang-Qi calls its removing blood stasis parasite killing, also this meaning ear.Promoting blood circulation to remove blood stasis, preventing the attack (or recurrence) of malaria, removing toxic substances, inducing diuresis to remove edema.Gather for abdominal mass, amenorrhea dysmenorrhea, malaria, sore throat, carbuncle, edema, pyretic stranguria.

Radix Glycyrrhizae Preparata is similar round or oval section, and the brown or taupe brown of surface red, micro-glossy, tangent plane is yellow to buff, and cambium ring is obvious, ray irradiation shape.Zhigancao decoction has benefiting qi and nourishing yin, and activate yang multiple arteries and veins therapeutic efficiency.

Rhizoma Polygonati Odorati (Polygonatumodoratum (Mill.) Druce) is Liliaceae herbaceos perennial.Rhizome horizontal walk, meat yellow-white, the most fibrous root of close life.Blade face is green, below Lycoperdon polymorphum Vitt.Flower axil is raw, and usual 1 ~ 3 clusters.Originate in Southwestern China area, but wild distribution is very wide.Cold-resistant, also resistance to the moon, happiness wet environment, suitable growth is in the chesson enriched containing humus." stem is tetanic, like bamboo arrow shaft, has joint for Collective Notes to the Canon of Materia medica cloud." therefore have the name of Rhizoma Polygonati Odorati.The rhizome of plant can hyoscine, and Chinese medicine name is also Rhizoma Polygonati Odorati, and autumn excavates, and cleans, shines to softness, repeatedly rub, and dry in the sun, to without hard-core, is dried, or steams thoroughly, rubs to translucent, dries, and cuts sheet or section use." book on Chinese herbal medicine justice ": " control lung dryness of the stomach heat, the diseases such as body fluid dries up, thirsty dry sensation in the pharynx, and excessiveness of stomach-fire, dry thirsty rapid digestion of food, polyorexia person, You Youjie is imitated ".

Herba Lycopi is the stem and leaf of dicotyledon medicine labiate lycopus lucidus.Effect: blood circulation promoting and blood stasis dispelling, inducing diuresis to remove edema.Cure mainly: control blood stasis amenorrhea, stimulate the menstrual flow, lump in the abdomen, the stagnant stomachache of the stasis of blood in puerperal, body and face edema, injury from falling down, incised wound, sore and toxic, edema, (edema of congestion retardance, the mutual resistance of the water stasis of blood is especially suitable for ascites.

Radix Et Caulis Acanthopanacis Senticosi cures mainly anemofrigid-damp arthralgia, lumbocrural pain, and muscles and bones flaccidity is soft, is slow in action, and body weakness with emaciation is weak, traumatic injury, and fracture, edema, beriberi, uncontrolled urination is itched.

Process Hirudo, bitter in the mouth, salty, property is put down, poisonous.Main Function is the removing blood stasis stasis of blood, eliminating stagnation alive.Hirudo contains hirudin, can delay and hinder blood coagulation, thus having blood coagulation resisting function.

Bulbus Allii Macrostemonis, function with cure mainly, activating YANG and eliminating stagnation, circulation of qi promoting intestinal stasis relieving.For thoracic obstruction pain, phlegm retention cough with asthma is heavy after letting out dysentery.

Radix Achyranthis Bidentatae, abnormal smells from the patient picric acid is put down, and cures mainly cold-damp flaccidity syndrome and arthralgia syndrome, spasm of the limbs, gonalgia unable to flex and stretch the extremities, and by vim and vigour, fire of getting mildewed in warm conditions is rotten, and clothes are made light of one's life by commiting suicide always resistance to for a long time.

Rhizoma Dioscoreae, common name Rhizoma Dioscoreae.Herbaceos perennial, stem is overgrow, and is often with purple, and tuber is cylindrical, and leaf is to life, avette or oval, flower milky, dioecism.Starch-containing and the protein of tuber.Rhizoma Dioscoreae-medicinal effects, plants name: Rhizoma Dioscoreae (y ù), Latin language formal name used at school: Dioscoreaopposita calls: Rhizoma Dioscoreae, triangle, RHIIZOMA DIOSCOREAE fr0m Henan of China, Rhizoma dioscoreae, medicated egg, native potato, mountain potato, jade prolong.Abnormal smells from the patient: (root) is sweet, warm, flat, nontoxic.English name: nagaimo, yamaimo, Chineseyam, Japanesemountainyam, Koreanyam; Compendium of Materia Medica " to record, Rhizoma Dioscoreae is Li Yu due to the Tang Dynasty ancestor, is taboo and change Rhizoma Dioscoreae into, is Zhao Shu again because of Song Yingzong, is taboo and change Rhizoma Dioscoreae into.Rhizoma Dioscoreae is one of composition medicine of LIUWEI DIHUANG WAN.Rhizoma Dioscoreae " book on Chinese herbal medicine is looked for the truth ": " enter in drugs for nourishing yin and should give birth to use, entering spleen reinforcing lung medicine should use by parching to brown." " this genus food, though gas temperature and put down, be the moon of spleen reinforcing lung.Be moistening fur, longue meat, sweet in the mouth is held concurrently salty, again can kidney tonifying reinforcing YIN-essence.", in the research of C-II induced arthritis in rats, Rhizoma Dioscoreae Aqueous extracts can significantly suppress Cu2+ to the Denaturation of gamma Globulin, shows to have antarthritic effect.Rhizoma Dioscoreae Aqueous extracts also can eliminate urine protein, mutant cell is had to the effect suppressing to produce.Invigorating the spleen and replenishing QI: Rhizoma Dioscoreae plants trace element because being rich in 18 seed amino acids and more than 10, and other mineral, so have strengthening the spleen and stomach, tonifying the lung kidney, invigorating the spleen and replenishing QI, spleen invigorating tonify deficiency, to reinforce the kidney the effects such as beneficial essence, beneficial heart and tranquilizing mind, saying of " spleen invigorating tonification, taste essence being reinforced the kidney, controlled all kinds of diseases and ailments, treat five kinds of strain and seven kinds of impairment " is had in Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.Quench one's thirst and promote the production of body fluid: this Rhizoma Dioscoreae has the effect of life of quenching one's thirst: Chinese traditional treatment asthenia is quenched one's thirst in (diabetes) prescription often has Rhizoma Dioscoreae single to use, or share with other medicines, and effect is better.Health care: because fresh Rhizoma dioscoreae is rich in multivitamin, aminoacid and mineral, can prevent and treat Human Lipid Metabolism abnormal, and arteriosclerosis, to safeguarding that insulin normal function also has certain effect, there is enhancing body immunity, beneficial heart and tranquilizing mind, rather cough Dingchuan, the health-care effects such as slow down aging.Skin care: Yuan Dynasty taste expert Li Gao says: " control xerosis cutis and moisten it with this thing." Li Shizhen (1518-1593 A.D.) is write: " Rhizoma Dioscoreae can moisten fur." to nourishes rough skin, vigorous and graceful skin care has unique curative effect to Rhizoma Dioscoreae.

Herba Typhonii gigantei: drying dampness to eliminate phlegm, expelling wind and relieving convulsion, detoxicating and resolving stagnation of pathogens.

Semen Coicis root: cure mainly 1. obstructive jaundice, especially the biliary tract ascarid person of blocking.Disease sees skin, yellow sclera, skin pruritus, and stool is potter's clay color, epigastrium angor etc.2. urinary system infection.Disease sees frequent micturition, urgent micturition, dysurea, and dribbling urination difficulty to the greatest extent.3. nephritis and non-specific edema.See lower limbs edema, dysuria.Commercially available Semen Coicis root tea can be selected.

Rhizoma Coptidis: heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion, and toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.

Other Chinese medicinal ingredients related to are through to be verified for many years, records and test gained, although their effect is different, choosing of each Chinese medicine is not random and be that length limit, and does not carry out exhaustive at this;

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition forms by following weight proportion: Herba Verbenae 10 parts, Herba Typhonii gigantei 1 part, Semen Coicis root 10 parts, Rhizoma Coptidis 10 parts, Auricularia 10 parts, 10 parts, Tremella, 10 parts, Oryza glutinosa, seed of Job's tears 10 parts, 10 ~ 20 parts, Semen Fagopyri Esculenti, Mel 5 ~ 50 parts, vinegar 50 ~ 100 parts.

1) commercially available super micron mill is selected;

Acute toxicity test:

Experimental subject: 20-25g healthy mice 10, male and female half and half

Experimental agents: the granule that the present invention makes.

Test method: according to 50 times of human administration, the dosage of 100 times gavages to white mice, negative control group gives the normal saline of matched doses, animal freely takes the photograph life and the death condition that animal observed by diseases caused by retention of fluid food, the white mice of survival was slaughtered after 10 days by administration, get the change of the indices of its its peripheral blood of whole blood assay, improvement karber's method (Journal of the Fourth Military Medical University, the 7th phase in 2002) is taked to ask LD ₅₀for 20000mg/kg, Toxicological evaluation is nontoxic level, has no side effect.

Model case is illustrated:

1, Lee's ××, man, 50 years old, the traditional Chinese medical science.Suffer from diabetes 10 years.Adopt mixed fugitive with intermediate-acting insulins injection for curing 1 year, Symptoms for often to emit cold sweat, dysphoria, 2003 time medical, survey blood glucose 17.1mmol/L.After taking 3 courses for the treatment of of Chinese medicine preparation of the present invention, glucose monitor data display is basicly stable, and intermediate-acting insulins consumption reduces half, and adhering to taking medicament of the present invention, is 5.4mmol/L when 2007, within 2012, is 3.5mmol/L.

2, Jiang's ××, female, 47 years old, finance section civil servant.Patient's readme hunger sensation is strong, xerostomia, polydipsia, polyuria, weight loss, and tinea pedis is serious, the symptoms such as long-term foot infection, and clinical diagnosis is diagnosed as type ii diabetes, and life-time service Jiangtangwan pill controls blood glucose.Because sensation trick always has prickling sensation, blurred vision, edema of leg, hospital admission after aggravation, blood fat raises on inspection, and low density lipoprotein, LDL raises obviously.Blood glucose 7.8mmol/L, glycolated hemoglobin 7.2%, alpha-fetoprotein (AFP) 5.2ng/ml, urine protein quantitation 239, two lower limb arteries visible sclerosis companion speckle.Obviously alleviate after taking Chinese medicine preparation of the present invention, continuing in after treatment.

3, execute ××, female, 55 years old, Environmental Protection Agency civil servant, suffer from diabetes more than 3 years, blurred vision, endocrine disturbance causes psychentonia irritability, and dry mouth and tougue is depressed, retardation of thinking, memory reduces, and movable minimizing, after taking Chinese medicine preparation of the present invention, obviously alleviates.

4, pipe ××, man, 60 years old, peasant, cardinal symptom was thirsty xerostomia, hyperhidrosis, blurred vision, substantially disappears by taking Chinese medicine preparation of the present invention 1 year symptom.

The present invention's prevention, treatment diabetes: blood plasma GMP140 is one of activation releasing product of platelet or endotheliocyte, the mensuration that research shows GMP140 content in blood plasma also can hematoblastic activation degree and thrombophilia in antimer, and TXB2 is the metabolic end product of XA2, the latter has very strong short platelet aggregation and contracting blood vessel function.Many report display diabetics blood plasma GMP140 and TXB2 level significantly increase, and illustrate that in diabetics body, platelet is in the state of activation.Platelet adhesion aggregation capability increases, and this is that diabetics arteriosclerosis occurs early and incidence rate is high and one of the reason of easy concurrent microangiopathies.Lumbrukinase likes to win the one group of acidic protein extracted Lumbricus from the compatriot of artificial breeding.The oral Lumbrukinase of diabetics 2 weeks rear plasma substance P levels obviously decline, and with matched group without significant difference, prompting Lumbrukinase is assembled platelet adhesion obvious inhibitory action.

Embodiment 2

1) commercially available super micron mill is selected;

Acute toxicity test:

Experimental subject: 20-25g healthy mice 10, male and female half and half

Experimental agents: the granule that the present invention makes.

Embodiment 3

1) commercially available super micron mill is selected;

Acute toxicity test:

Experimental subject: 20-25g healthy mice 10, male and female half and half

Experimental agents: the granule that the present invention makes.

Described herein is that the numerical range related in embodiment is all through many experiments gained, and each point can realize, and be not confined to end points or terminal, length limit, and does not enumerate at this.

Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims

1. prevent, treat a Chinese medicine composition for diabetes, it is characterized in that described Chinese medicine composition forms by following weight proportion: Herba Verbenae 10 ~ 20 parts, Herba Typhonii gigantei 1 ~ 2 part, Semen Coicis root 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Auricularia 10 ~ 20 parts, 10 ~ 20 parts, Tremella, 10 ~ 20 parts, Oryza glutinosa, seed of Job's tears 10 ~ 20 parts, 10 ~ 20 parts, Semen Fagopyri Esculenti, Mel 5 ~ 50 parts, vinegar 50 ~ 100 parts.

2. prevent, treat a Chinese medicine composition for diabetes, it is characterized in that described Chinese medicine composition forms by following weight proportion: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts.

3. prevent, treat a Chinese medicine composition for diabetes, it is characterized in that described formula forms by following weight: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts; Supplementary element 5 ~ 10 parts, described supplementary element is Lumbrukinase.

4. prevent, treat a Chinese medicine composition for diabetes, it is characterized in that described formula forms by following weight: Herba Verbenae 15 parts, Herba Typhonii gigantei 1.5 parts, Semen Coicis root 15 parts, Rhizoma Coptidis 15 parts, Auricularia 15 parts, 15 parts, Tremella, 15 parts, Oryza glutinosa, seed of Job's tears 15 parts, 15 parts, Semen Fagopyri Esculenti, Mel 28 parts, vinegar 75 parts; Supplementary element 5 ~ 10 parts, described supplementary element is the mixture of Chinese crude drug Radix Tylophorae arenicolae, Chinese crude drug leaf of Cyclocarya paliurus Iljinskaja, Chinese crude drug Conocephalum conicum(L．) Dumortier．, and Mixing ratio by weight example is 1:1 ~ 2:1.

5. the Chinese medicine composition of a kind of prevention according to claim 4, treatment diabetes, is characterized in that: described Lumbrukinase is prepared by following steps:

2) Lumbricus cleaning: optional step 1) Lumbricus alive that cultivates, the Lumbricus that removal impurity and dead go bad, Lumbricus is put into the water 10 ~ 15 minutes that pH value is 4.5 ~ 6, add 60 ~ 65g Sal simultaneously, the 1-2 hour of illumination simultaneously, impels Lumbricus get rid of dirt in body and clean Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, uses the warm water washing of 25 ~ 27 DEG C, wash away excessive moisture and the impurity of outer surface;

3) slurrying: slurrying: the ethanol sodium citrate buffer solution that the pH value adding Lumbricus volume 3-6 times is 5.0 ~ 5.3, concentration is 0.1mol/L or pH value are 5.0 ~ 5.3, concentration is the sodium phosphate buffer of 0.1mol/L, after above-mentioned Lumbricus being carried out cell breakage with pearl mill, colloidal mill or high pressure homogenizer, the sodium citrate adding above-mentioned parts by weight carries out fully stirring making homogenate;

4) by step 3) homogenate that obtains is by filtering or the centrifugal removing of sedimentation centrifuge thick slag wherein, and the homogenate after slagging-off, adopts 5000 turns of centrifuge per minute 16 ~ 20 minutes or 0.45 micron pore size membrane filtration process, obtains clear liquor;

5) saltout: step 4) add ammonium sulfate by 35% saturated concentration in gained clear liquor, stir leave standstill 50 minutes after dissolving completely mixed liquor;

6) centrifugal ultrafiltration: with vertical high speed centrifugal machine through high speed centrifugation by step 5) in mixed liquor thin slag remove, obtained clear liquid, then by 50000dt membrane ultrafiltration, obtain ultrafiltrate; 7) chromatographic column is separated: adopt anion-exchange chromatography post or affinity column separating step 6) ultrafiltrate that obtains, with petroleum ether, 0.005 ~ 1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of Peak Activity part; 8) sterilize: by above-mentioned steps 7) chromatography effluent put into the sealed sterilised container of carrying pipe, in container, high pressure nitrogen gas is injected through conduit, machinery fully stirs pending clear liquor 5 ~ 8min simultaneously, release pressure is exported through conduit, after pressure reduces, nitrogen solubility reduces rapidly, the rapid loss of a large amount of nitrogen gas in container; Then filter removal of contamination, precipitate 10 hours and obtain supernatant; 9) by step 8) lyophilization of gained supernatant, in subzero 28 ~ 35 degree of freezer dryers freezing 5 ~ 8 hours, then under the vacuum of 0.1mmHg, be warming up to 25 ~ 30 DEG C, dry 8 hours; Continue to be warming up to 35 ~ 39 DEG C, vacuum drying 5-6 hour, obtains powdery product.

6. the Chinese medicine composition of a kind of prevention according to claim 4, treatment diabetes, is characterized in that: it is used to the medicament prepared treatment or prevent diabetes.

7. the Chinese medicine composition of a kind of prevention according to claim 4, treatment diabetes, is characterized in that: it is used to prepare treatment or the food that prevents diabetes or health product.

8. the Chinese medicine composition of a kind of prevention according to claim 1-4, treatment diabetes, is characterized in that: it is used to prepare the medicament for the treatment of or the complication that prevents diabetes.

9. the preparation method of the Chinese medicine composition of a kind of prevention according to claim 2, treatment diabetes, is characterized in that step is as follows:

1) commercially available super micron mill is selected;