CN103893620A

CN103893620A - Chinese medicinal composition for preventing and treating diabetes and preparation method thereof

Info

Publication number: CN103893620A
Application number: CN201410055138.1A
Authority: CN
Inventors: 李正朝
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-02-18
Filing date: 2014-02-18
Publication date: 2014-07-02
Anticipated expiration: 2034-02-18
Also published as: CN103893620B

Abstract

The invention discloses a Chinese medicinal composition for preventing and treating diabetes and a preparation method thereof. The Chinese medicinal composition consists of the following components in parts by weight: 10-20 parts of vervain, 1-2 parts of typhonium giganteum, 10-20 parts of barley root, 10-20 parts of coptis root, 10-20 parts of black fungus, 10-20 parts of white fungus, 10-20 parts of sticky rice, 10-20 parts of barley rice, 10-20 parts of buckwheat, 5-50 parts of honey and 50-100 parts of vinegar. Natural animal and plant medicinal materials are taken as raw material sources, and diabetics syndrome and nephrotic syndrome are treated by using the comprehensive function of various Chinese medicinal components. As proved by acute toxicity test and long-term toxicity test, the Chinese medicinal composition is nontoxic to human body and good in medicinal effect and has the effects of improving the hypercoagulable state, improving blood fat, reducing blood sugar, dissolving thrombi, dredging microcirculation and improving the immunity of organisms.

Description

Chinese medicine composition of a kind of prevention, treatment diabetes and preparation method thereof

Technical field

The invention belongs to the field of Chinese medicines, relate in particular to Chinese medicine composition of a kind of prevention, treatment diabetes and preparation method thereof.

Background technology

The diabetics of China is numerous at present, and diabetes are one group of metabolic diseases take hyperglycemia as feature.Hyperglycemia be due to defect of insulin secretion or its biological agent impaired, or both have concurrently and cause.Long-standing hyperglycemia when diabetes, causes various tissues, particularly chronic lesion, the dysfunction of eye, kidney, heart, blood vessel, nerve.Patient stands ailing torment for a long time, mostly takes in the market Western medicine, can only control, and can not effect a radical cure, and even causes even renal failure of other complication because of long-term taking Western medicine.The concept of effecting a permanent cure in order to the present invention proposes Chinese medicine, through long-term R & D, pharmacological evaluation and clinical verification, has drawn pure natural medical of the present invention.

In addition, for patient is prevented, when treating diabetes, physical function does not fail, and has also preferably added Lumbrukinase.Lumbrukinase is a kind of from extracting the histone hydrolase being isolated in Lumbricus body, its main component is plasmin and plasminogen activator, these two kinds of compositions are by the former dual function with activating fibrinolysin of direct fibrin degradation, can reduce fibrinogen content and hematoblastic aggregation rate, strengthen Fibrinolytic Activities, blood coagulation and fibrinolytic after correction cerebral infarction are abnormal, to diseases such as treatment angina pectoris, acute cerebral infarction, hyperlipidemia, hypertension, alleviate patient's slight illness and all there is good curative effect.Lumbrukinase is to have most at present one of fibrinolytic medicine of DEVELOPMENT PROSPECT, no matter intravenous injection or oral administration, and thrombolytic effect is all clearly.

Lumbrukinase, along with the dissolving of thrombosis, softening, the microcirculatory improvement of blood vessel, reduced blood viscosity, make abnormal hemorheology index recover normal, Microcirculation of Nailfold is detected by extremely transferring to normal, thereby eliminated the hidden danger that cordis and cerebral accident occurs, reduce blood glucose simultaneously, the positive source comprehensive effect optimum due to active earthworm protein improved the multiple function of human body, the interior environment that has improved body, has promoted the recovery of islet function, has certain blood sugar reducing function after taking this product.

Lumbrukinase extracts from Pheretima, and Pheretima has another name called Lumbricus, and [3] Pheretima is one of important Chinese crude drug of China.In 67 kinds of animal drugs recording in Chinese materia medica monograph Shennong's Herbal the earliest, just there is Lumbricus.Classify low-grades as at Shennong's Herbal, there is heat clearing away arresting convulsion, dredging collateral, relieving asthma, effect of diuresis.After normal parch for diseases such as high heat, coma, infantile convulsion tic, arthralgia, dyspnea and cough due to lung-heat, oliguria edema, hypertension.Main containing several amino acids.Because raw product fishy smell is too heavy, generally need be through parch therefore be used as medicine.TAO Hong-Jing meaning: " if take dry earthworm, must endure and consider to be worth doing ".Tradition is with Pheretima, and method for making is a lot, mainly contains medicine system, vinegar system, boils, the method such as processed with wine, oil system, Pulvis concha Mactrae parch, processed with salt, makes its quality pine steep crisp, detoxification, correct stink and be convenient to fried taking.

Pheretima is except having better nutritivity value, and it also has a lot of health cares, can strengthen the premunition of human body, improves people's health level.In recent ten years, Pheretima is carried out to large quantity research chemical, pharmacology aspect both at home and abroad, find that Pheretima not only contains abundant nutritional labeling, also contain multiple pharmacological component, there is many-sided pharmacological action, relate to each system of human body as cardiovascular system, blood system, immune system, central nervous system, respiratory system, digestive system etc.Especially unify aspect blood system at cardiovascular system, Pheretima is prophylaxis of hypertension, myocardial infarction, arrhythmia, apoplexy thromboembolism well.Application

At present, the application of China's Chinese medicine has more and more obtained the approval of international community, its multiple medical material is evident in efficacy, medicine for blood sugar lowering is various especially, but real evident in efficacy in practical application, only have several, such as Herb Gynostemmae Pentaphylli, Semen Cassiae etc., and the application mentions obtaining Herba Verbenae, Herba Typhonii gigantei, apply little.The applicant finds in the leaching process at Lumbrukinase in actual production and application, feed Lumbricus with Chinese medicine in advance, natural compound a certain amount of blood-sugar lowering tcm drug, the Lumbrukinase of locating of compound extraction has remarkable result aspect blood sugar lowering, in blood fat reducing and blood pressure lowering, have too significant curative effect, successful is strengthened.

Summary of the invention

For realizing object of the present invention, concrete scheme is as follows:

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition is by following weight proportion composition: 10～20 parts of Herba Verbenae, 1～2 part of Herba Typhonii gigantei, 10～20 parts of Semen Coicis roots, 10～20 parts of Rhizoma Coptidis, 10～20 parts of Auricularia, 10～20 parts, Tremella, 10～20 parts, Oryza glutinosa, 10～20 parts of seed of Job's tears, 10～20 parts, Semen Fagopyri Esculenti, 5～50 parts of Mel, 50～100 parts of vinegar.

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition is by following weight proportion composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar.

A Chinese medicine composition for prevention, treatment diabetes, described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar;

Described compositions configuration step one, to take by said ratio be 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, by super micron mill, after pulverizing, make superfine powder to 100 order stand-by; Prepared micropowder is put into container, add pure water to boil and extract 2 times, add for the first time 15-20 times of pure water to soak 1 hour, heated and boiled 1 hour, cooling precipitation 1-1.5 hour, gets supernatant; Add for the second time 5-8 times of pure water heated and boiled 20-40 minute, cooling precipitation 1-1.5 hour, gets supernatant; Merge for the first time with secondary supernatant, filter, obtain filtrate for later use;

Described seed of Job's tears are the seed of Job's tears below 8wt% by dried water content, are placed in parch pot in 120～150 ℃ of parches 20～55 minutes, until surperficial yellowish-brown takes out for subsequent use; Described Semen Fagopyri Esculenti is the seed of Job's tears below 8wt% by dried water content, is placed in parch pot in 120～150 ℃ of parches 20～55 minutes, until surperficial yellowish-brown takes out for subsequent use; Described Herba Verbenae comprises Herba Verbenae stem and leaf, flower and the seed after cleaning up and drying;

Step 2, according to proportioning, take 28 parts of Mel, 75 parts of vinegar, with vinegar by Mel evenly punching and, obtain honey vinegar solution, for subsequent use;

The raw material that above-mentioned steps one, two is obtained mixes, the paste semi-finished product that then stir evenly, concentrating under reduced pressure, sterilizing obtain the Chinese medicine composition of described prevention, treatment diabetes at 60 ℃ Celsius;

By the granule processed semi-finished product of described Chinese medicine composition, pill, tablet, thick soup or add and make portable capsule in capsule.

A Chinese medicine composition for prevention, treatment diabetes, described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar; 5～10 parts of supplementary elements, described supplementary element is Lumbrukinase.

A Chinese medicine composition for prevention, treatment diabetes, described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar; 5～10 parts of supplementary elements, described supplementary element is the mixture of Chinese crude drug Radix Tylophorae arenicolae, Chinese crude drug leaf of Cyclocarya paliurus Iljinskaja, Chinese crude drug Conocephalum conicum(L．) Dumortier．, weight mixed proportion is 1:1～2:1.

A Chinese medicine composition for prevention, treatment diabetes, described Lumbrukinase is prepared by following steps:

1) vermiculture: select a day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Pheretima pingi, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting starts to feed Chinese medicine composite powder for 30 days in advance, and described Chinese medicine composite powder is by weight ratio: 5 parts of Radix Glycyrrhizae Preparatas, 3 parts of Radix Achyranthis Bidentataes, process 2 parts of Hirudos, 2 parts of Herba Lycopi, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 2 parts of Rhizoma Polygonati Odorati, 1 part of Bulbus Allii Macrostemonis, 1.5 parts of Fructus Lyciis, 1 part of Radix Et Caulis Acanthopanacis Senticosi, 1 part of Cordyceps, 1 part of Radix Puerariae; Described Chinese medicine composite powder is to be crushed to through super micron mill the powder that 300～450 orders are made;

2) Lumbricus is cleaned: optional step 1) Lumbricus alive that cultivates, remove impurity and the rotten Lumbricus of dead, it is 4.5～6 water 10～15 minutes that Lumbricus is put into pH value, adds 60～65g Sal simultaneously, the 1-2 hour of illumination simultaneously, impels Lumbricus get rid of the interior dirt of body and clean Lumbricus body surface; Lumbricus alive after cleaning is carried out to drip washing, use the warm water washing of 25～27 ℃, wash away excessive moisture and the impurity of outer surface;

3) slurrying: slurrying: add the sodium phosphate buffer that Lumbricus volume 3-6 pH value is doubly 5.0～5.3, concentration is 0.1mol/L ethanol sodium citrate buffer solution or pH value are 5.0～5.3, concentration is 0.1mol/L, above-mentioned Lumbricus is carried out after cell breakage with pearl mill, colloidal mill or high pressure homogenizer, add the sodium citrate of above-mentioned parts by weight to carry out fully stirring and making homogenate.

4) homogenate step 3) being obtained is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, and the homogenate after slagging-off, adopts per minute 5000 to leave centrifugal 16～20 minutes of scheming or 0.45 micron pore size membrane filtration processing, obtains clear liquor;

5) saltout: in step 4) gained clear liquor, add ammonium sulfate by 35% saturated concentration, stir and dissolve latter standing 50 minutes to obtain mixed liquor completely;

6) centrifugal ultrafiltration: thin slag in mixed liquor in step 5) is removed through high speed centrifugation with vertical high speed centrifugal machine, made clear liquid, then by 50000dt membrane ultrafiltration, obtain ultrafiltrate; 7) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 6) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of Peak Activity part; 8) sterilization: by above-mentioned steps 7) chromatography effluent put into the sealed sterilised container of carrying pipe, in container, inject high pressure nitrogen gas through conduit, machinery fully stirs pending clear liquor 5～8min simultaneously, through conduit output release pressure, after pressure decreased, nitrogen solubility reduces rapidly, the rapid loss of a large amount of nitrogen gas in container; Then filter removal of contamination, precipitate 10 hours and obtain supernatant; 9), by the lyophilization of step 8) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5～8 hours, then, under the vacuum of 0.1mmHg, be warming up to 25～30 ℃, dry 8 hours; Continue to be warming up to 35～39 ℃, vacuum drying 5-6 hour, obtains powdery product.

Preferably, described a kind of prevention, the Chinese medicine composition for the treatment of diabetes, is characterized in that: it is used to, and preparation is treated or the medicament of prevent diabetes.

Preferably, described a kind of prevention, the Chinese medicine composition for the treatment of diabetes, is characterized in that: it is used to, and preparation is treated or food or the health product of prevent diabetes.

Preferably, described a kind of prevention, the Chinese medicine composition for the treatment of diabetes, is characterized in that: it is used to, and preparation is treated or the medicament of prevent diabetes complication.

Preferably, the preparation method of described a kind of prevention, the Chinese medicine composition for the treatment of diabetes, is characterized in that step is as follows:

1) select commercially available super micron mill;

2) take 15 parts of 15 parts, 15 parts, 15 parts of 15 parts of 15 parts of 1.5 parts of 15 parts of Herba Verbenae, Herba Typhonii gigantei, Semen Coicis roots, Rhizoma Coptidis, Auricularia, Tremella, Oryza glutinosa, seed of Job's tears, 15 parts, the Semen Fagopyri Esculenti of respective amount, by super micron mill, after pulverizing, make superfine powder to 100 order stand-by; Prepared micropowder is put into container, add pure water to boil and extract 2 times, add for the first time 15-20 times of pure water to soak 1 hour, heated and boiled 1 hour, cooling precipitation 1-1.5 hour, gets supernatant; Add for the second time 5-8 times of pure water heated and boiled 20-40 minute, cooling precipitation 1-1.5 hour, gets supernatant; Merge for the first time with secondary supernatant, filter, obtain filtrate for later use;

Described Chinese medicinal ingredients is through verifying for many years, record and test gained, although their effect is different, but choosing of each Chinese medicine is not to be arbitrarily, the applicant is according to the proportioning of the each composition of test gained for many years, be not by raw material effect simple superposition of each composition, because may there is the multiple interactivelies such as interpromoting relation in five elements, mutual reinforcement between, mutual restraint between two drugs or the mutual-detoxication between various raw material of Chinese medicine, and the applicant has selected above-mentioned raw materials accordingly according to this principle exactly, and determine the best proportioning between them, therefore, the application has significant meaning.

Beneficial effect:

It is raw material sources that the present invention selects natural animal and plant medical material, utilize the comprehensive function of each natural constituents, prevention, treatment diabetic complication, through acute toxicology and long-term toxicological test, prove human body nonhazardous effect, its drug effect is high, can improve high blood coagulation state, improves blood fat, reduce blood glucose, thrombus, dredging microcirculation, improves immunity of organisms simultaneously.

The granule that the present invention makes, composition properties and characteristics is pitchy, powdery, has bitterness, soluble in water.Show through safety toxicology test: LD50 is 20000mg/kg, Toxicological evaluation is nontoxic level, has no side effect.

Drink through human experiment, every day, 1-2 wrapped, every bag 5g.In 150 people, have obesity (with hyperlipidemia), hypertension, hyperglycemia symptom patient, everyone drinks 30-60 days, and drug withdrawal 30-60 days.

50 hyperglycemic patients have been obtained to good significant blood sugar lowering, lipid-lowering effect; Experimenter drank after 30 days, the TC 1.12mmol/L that on average declines, HDL rising 0.80mmol/L, the effect of TC and rising HDL wherein obviously declines, its effect is higher than vitamin E, the average rate of decrease that plays its glucose is 12.5%, and the average rate of decrease of fructosamine is to check its blood glucose value all to reach normal level after 26.3%, 30 day.Result of the test shows: the health product effect the present invention relates to is remarkable., intrinsic heat dryness-transformation stagnant for suffering from a deficiency of the kidney of diabetes quenched one's thirst for causing, in disorder of emotion, stomach etc. pathogenic because of, take YIN nourishing loose stagnant, soothing the liver promote the production of body fluid, regulate the flow of vital energy eliminating evil as aim, ask removing heat-phlegm, loose strongly fragrantly quench one's thirst, the merit of dryness and dampness coordinating each other, good absorbing effect, evident in efficacy, through clinical verification, be applicable to alleviation and the treatment of diabetes and diabetic complication.

In addition, wherein obesity patient's (with hyperlipidemia symptom) 50 people, through check fasting plasma lipid, T-CHOL CT, triglyceride TG and high density lipoprotein HDL, contrast test before and after drinking, carry out again curative effect comparison: test is drunk 30 days, and experimenter TC, TG significantly decline, and HDL obviously rises, TC declines respectively 32.1%, 43.2%, 55.4%;

In addition, wherein 50 hypertension symptom persons, drank after 30 days, obtained good blood pressure lowering effect simultaneously, and mean blood pressure declines 30%, blood pressure drops amplitude: systolic pressure decline 3.16kpa, and diastolic pressure decline 2.60%kpa, its hypotensive effect is remarkable;

Show through pharmacological testing: it is raw material sources that the present invention selects natural animal and plant medical material, utilize the comprehensive function of each Chinese medicinal components, prevention, treatment diabetic syndrome, through acute toxicology and long-term toxicological test, prove human body nonhazardous effect, its drug effect is high, can improve high blood coagulation state, improves blood fat, reduce blood glucose, thrombus, dredging microcirculation, improves immunity of organisms simultaneously.

Specific embodiment

Embodiment 1

Herba Verbenae HerbaVerbenae (English) EuropeanVerbenaHerb, another name Herba lespedezae pilosae, purple top Agrimony, wild catmint.

Herba Verbenae is originally the medicine of removing heat from blood removing blood stasis.Bottom abscess person, the utmost point of heat in blood, furthermore damp and hot, thus the turbid skin ulcer that forms of blood stains, and have worm also.The cool pyrolysis of blood, the broken and row of dirty person, waste and be not recovered.CHEN Zang-Qi is called its removing blood stasis parasite killing, also this meaning ear.Promoting blood circulation to remove blood stasis, preventing the attack (or recurrence) of malaria, removing toxic substances, inducing diuresis to remove edema.Gather amenorrhea dysmenorrhea, malaria, sore throat, carbuncle, edema, pyretic stranguria for abdominal mass.

Radix Glycyrrhizae Preparata is similar round or oval section, the brown or taupe brown of surface red, and micro-glossy, tangent plane yellow is to buff, and cambium ring is obvious, ray irradiation shape.Zhigancao decoction has benefiting qi and nourishing yin, and multiple arteries and veins therapeutic efficiency activates yang.

Rhizoma Polygonati Odorati (Polygonatumodoratum (Mill.) Druce) is Liliaceae herbaceos perennial.Rhizome horizontal walk, meat yellow-white, the most fibrous roots of close life.Blade face green, below Lycoperdon polymorphum Vitt.Flower axil is raw, and common 1～3 clusters.Originate in Southwestern China area, but wild distribution is very wide.Cold-resistant, also resistance to the moon, happiness wet environment, suitable growth is in containing the abundant chesson of humus." stem is tetanic, like bamboo arrow shaft, has joint for Collective Notes to the Canon of Materia medica cloud.Therefore " there is the name of Rhizoma Polygonati Odorati.The rhizome of plant can hyoscine, and Chinese medicine name is also Rhizoma Polygonati Odorati, and excavate autumn, cleans, and shines to softness, repeatedly rubs, and dry in the sun, to without hard-core, is dried, or after steaming thoroughly, rubs to translucent, dries, and cuts sheet or section is used." book on Chinese herbal medicine justice ": " controlling lung dryness of the stomach heat, the disease such as body fluid dries up, thirsty dry sensation in the pharynx, and excessiveness of stomach-fire, dry thirsty rapid digestion of food, polyorexia person, You Youjie effect ".

Herba Lycopi is the stem and leaf of dicotyledon medicine labiate lycopus lucidus.Effect: blood circulation promoting and blood stasis dispelling, inducing diuresis to remove edema.Cure mainly: control blood stasis amenorrhea, stimulate the menstrual flow, lump in the abdomen, the stagnant stomachache of the stasis of blood in puerperal, body and face edema, injury from falling down, incised wound, sore and toxic, edema, (edema of congestion retardance, the mutual resistance of the water stasis of blood is especially suitable for ascites.

Radix Et Caulis Acanthopanacis Senticosi cures mainly anemofrigid-damp arthralgia, lumbocrural pain, and muscles and bones flaccidity is soft, is slow in action, a little less than body weakness with emaciation, traumatic injury, fracture, edema, beriberi, uncontrolled urination is itched.

Process Hirudo, bitter in the mouth, salty, property is flat, poisonous.Main Function is the removing blood stasis stasis of blood alive, eliminating stagnation.Hirudo contains hirudin, can delay and hinder blood coagulation, thereby have blood coagulation resisting function.

Bulbus Allii Macrostemonis, function with cure mainly, activating YANG and eliminating stagnation, circulation of qi promoting intestinal stasis relieving.For thoracic obstruction pain, phlegm retention cough with asthma, lets out after dysentery heavy.

Radix Achyranthis Bidentatae, abnormal smells from the patient picric acid is flat, cures mainly cold-damp flaccidity syndrome and arthralgia syndrome, spasm of the limbs, gonalgia unable to flex and stretch the extremities, by vim and vigour, the fire of getting mildewed in warm conditions is rotten, and clothes are made light of one's life by commiting suicide resistance to old for a long time.

Rhizoma Dioscoreae, common name Rhizoma Dioscoreae.Herbaceos perennial, stem is overgrow, and is often with purple, and tuber is cylindrical, and leaf is to life, avette or oval, flower milky, dioecism.Starch-containing and the protein of tuber.Rhizoma Dioscoreae-medicinal part, plants name: Rhizoma Dioscoreae (y ù), Latin language formal name used at school: Dioscoreaopposita another name: Rhizoma Dioscoreae, triangle, RHIIZOMA DIOSCOREAE fr0m Henan of China, Rhizoma dioscoreae, medicated egg, native potato, mountain potato, jade prolong.Abnormal smells from the patient: (root) is sweet, warm, flat, nontoxic.English name: nagaimo, yamaimo, Chineseyam, Japanesemountainyam, Koreanyam; Compendium of Materia Medica " to record, Rhizoma Dioscoreae is because the Tang Dynasty ancestor is Li Yu, for taboo changes Rhizoma Dioscoreae into, again because Song Yingzong is Zhao Shu, for taboo changes Rhizoma Dioscoreae into.Rhizoma Dioscoreae is one of composition medicine of LIUWEI DIHUANG WAN.Rhizoma Dioscoreae " book on Chinese herbal medicine is looked for the truth ": " enter in drugs for nourishing yin and should give birth to use, entering spleen reinforcing lung medicine should use by parching to brown." " this genus food, though gas is warm and flat, is the moon of spleen reinforcing lung.Can moisten fur, longue meat, sweet in the mouth is held concurrently salty, again can kidney tonifying reinforcing YIN-essence.", in the research of C-II induced arthritis in rats, Rhizoma Dioscoreae water extraction liquid can significantly suppress the Denaturation of Cu2+ to gamma Globulin, shows to have antarthritic effect.Rhizoma Dioscoreae water extraction liquid also can be eliminated urine protein, mutant cell is had to the effect that suppresses generation.Invigorating the spleen and replenishing QI: Rhizoma Dioscoreae is planted trace element because being rich in 18 seed amino acids and more than 10, and other mineral, so have strengthening the spleen and stomach, tonifying the lung kidney, invigorating the spleen and replenishing QI, spleen invigorating tonify deficiency, the effects such as beneficial essence, beneficial heart and tranquilizing mind of reinforcing the kidney, in Li Shizhen (1518-1593 A.D.) Compendium of Material Medica, there is saying of " all kinds of diseases and ailments are reinforced the kidney, controlled to spleen invigorating tonification, taste essence, treats five kinds of strain and seven kinds of impairment ".Quench one's thirst and promote the production of body fluid: this Rhizoma Dioscoreae has effect of the life of quenching one's thirst: Chinese traditional treatment asthenia is quenched one's thirst in (diabetes) prescription often has Rhizoma Dioscoreae single to use, or share with other medicines, and effect is better.Health care: because fresh Rhizoma dioscoreae is rich in multivitamin, aminoacid and mineral, can prevent and treat Human Lipid Metabolism abnormal, and arteriosclerosis, to safeguarding that insulin normal function also has certain effect, there is enhancing body immunity, beneficial heart and tranquilizing mind, rather cough the health-care effects such as Dingchuan, slow down aging.Skin care: Yuan Dynasty taste expert Li Gao says: " control xerosis cutis and moisten it with this thing." Li Shizhen (1518-1593 A.D.) is write: " Rhizoma Dioscoreae can be moistened fur." Rhizoma Dioscoreae is to nourishes rough skin, and vigorous and graceful skin care has unique curative effect.

Herba Typhonii gigantei: drying dampness to eliminate phlegm, expelling wind and relieving convulsion, detoxicating and resolving stagnation of pathogens.

Semen Coicis root: cure mainly 1. obstructive jaundice, especially the biliary tract ascarid person of blocking.Disease is seen skin, yellow sclera, skin pruritus, and stool is potter's clay color, epigastrium angor etc.2. urinary system infection.Disease is seen frequent micturition, urgent micturition, dysurea, and dribbling urination is to the greatest extent difficult.3. nephritis and non-specific edema.See lower limbs edema, dysuria.Can select commercially available Semen Coicis root tea.

Rhizoma Coptidis: heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.

Other Chinese medicinal ingredients that relate to are through verifying for many years, record and test gained, although their effect is different, choosing of each Chinese medicine is not to be arbitrarily that length is limit, and does not carry out exhaustive at this;

A Chinese medicine composition for prevention, treatment diabetes, described Chinese medicine composition is by following weight proportion composition: 10 parts of Herba Verbenae, 1 part of Herba Typhonii gigantei, 10 parts of Semen Coicis roots, 10 parts of Rhizoma Coptidis, 10 parts of Auricularia, 10 parts, Tremella, 10 parts, Oryza glutinosa, 10 parts of seed of Job's tears, 10～20 parts, Semen Fagopyri Esculenti, 5～50 parts of Mel, 50～100 parts of vinegar.

1) select commercially available super micron mill;

Acute toxicity test:

Experimental subject: 10 of 20-25g healthy mices, male and female half and half

Experimental agents: the granule that the present invention makes.

Test method: the dosage according to 50 times of human body administration, 100 times gavages to white mice, negative control group gives the normal saline of matched doses, animal is freely taken the photograph life and the death condition that diseases caused by retention of fluid food is observed animal, administration after 10 days is slaughtered the white mice of survival, get the variation of the indices of its its peripheral blood of whole blood assay, take to improve karber's method (Journal of the Fourth Military Medical University, the 7th phase in 2002) and ask LD ₅₀for 20000mg/kg, Toxicological evaluation is nontoxic level, has no side effect.

Model case is given an example:

1, Lee × ×, man, 50 years old, the traditional Chinese medical science.Suffer from diabetes 10 years.Adopt mixed fugitive and intermediate-acting insulins injection for curing 1 year, Symptoms is for often emitting cold sweat, dysphoria, 2003 when medical, survey blood glucose 17.1mmol/L.Take after 3 courses for the treatment of of Chinese medicine preparation of the present invention glucose monitor data and show basicly stablely, intermediate-acting insulins consumption reduces half, adheres to taking medicament of the present invention, is 5.4mmol/L 2007 time, within 2012, is 3.5mmol/L.

2, Jiang × ×, female, 47 years old, finance section civil servant.Patient's readme hunger sensation is strong, xerostomia, polydipsia, polyuria, weight loss, and the symptom such as tinea pedis is serious, the infection of long-term foot, clinical diagnosis is diagnosed as type ii diabetes, life-time service Jiangtangwan pill control blood glucose.Because sensation trick always has prickling sensation, blurred vision, edema of leg, hospital admission after aggravation, blood fat raises on inspection, and low density lipoprotein, LDL raises obviously.Blood glucose 7.8mmol/L, glycolated hemoglobin 7.2%, alpha-fetoprotein (AFP) 5.2ng/ml, urine protein quantitation 239, the visible arteriosclerosis companion of two lower limb speckle.After taking Chinese medicine preparation of the present invention, obviously alleviate, continuing in after treatment.

3, execute × ×, female, 55 years old, the civil servant of Environmental Protection Agency, suffered from diabetes more than 3 years, blurred vision, endocrine disturbance causes psychentonia irritability, and dry mouth and tougue is depressed, retardation of thinking, memory reduces, and movable minimizing, takes after Chinese medicine preparation of the present invention, obviously alleviates.

4, pipe × ×, man, 60 years old, peasant, cardinal symptom is thirsty xerostomia, hyperhidrosis, blurred vision, disappears substantially by taking 1 year symptom of Chinese medicine preparation of the present invention.

The present invention's prevention, treatment diabetes: blood plasma GMP140 is one of activation releasing product of platelet or endotheliocyte, the mensuration that research shows GMP140 content in blood plasma also can antimer in hematoblastic activation degree and thrombophilia, and the metabolic end product that TXB2 is XA2, the latter has very strong short platelet aggregation and contracting blood vessel function.Many reports show that diabetics blood plasma GMP140 and TXB2 level significantly increase, and illustrate in diabetics body that platelet is in the state of activation.Platelet adhesion aggregation capability increases, and this is that diabetics arteriosclerosis occurs early and incidence rate is high and one of the reason of easy concurrent microangiopathies.Lumbrukinase is to like to win from artificial breeding's compatriot the one group of acidic protein extracting Lumbricus.After the oral Lumbrukinase of diabetics 2 weeks, blood plasma TXB2 level obviously declines, and with matched group without significant difference, prompting Lumbrukinase is assembled and is had obvious inhibitory action platelet adhesion.

Embodiment 2

1) select commercially available super micron mill;

Acute toxicity test:

Experimental subject: 10 of 20-25g healthy mices, male and female half and half

Experimental agents: the granule that the present invention makes.

Embodiment 3

1) select commercially available super micron mill;

Acute toxicity test:

Experimental subject: 10 of 20-25g healthy mices, male and female half and half

Experimental agents: the granule that the present invention makes.

The numerical range relating in the embodiment of being described herein is all through many experiments gained, and each point can be realized, and is not confined to end points or terminal, and length is limit, and does not enumerate at this.

Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims

1. a Chinese medicine composition for prevention, treatment diabetes, is characterized in that described Chinese medicine composition is by following weight proportion composition: 10～20 parts of Herba Verbenae, 1～2 part of Herba Typhonii gigantei, 10～20 parts of Semen Coicis roots, 10～20 parts of Rhizoma Coptidis, 10～20 parts of Auricularia, 10～20 parts, Tremella, 10～20 parts, Oryza glutinosa, 10～20 parts of seed of Job's tears, 10～20 parts, Semen Fagopyri Esculenti, 5～50 parts of Mel, 50～100 parts of vinegar.

2. a Chinese medicine composition for prevention, treatment diabetes, is characterized in that described Chinese medicine composition is by following weight proportion composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar.

3. the Chinese medicine composition of a kind of prevention according to claim 2, treatment diabetes, is characterized in that described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar;

Described compositions configuration step one, to take by said ratio be 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, by super micron mill, after pulverizing, make superfine powder to 100 order stand-by; Prepared micropowder is put into container, add pure water to boil and extract 2 times, add for the first time 15-20 times of pure water to soak 1 hour, heated and boiled 1 hour, cooling precipitation 1-1.5 hour, gets supernatant; Add for the second time 5-8 times of pure water heated and boiled 20-40 minute, cooling precipitation 1-1.5 hour, gets supernatant; Merge for the first time with secondary supernatant, filter, obtain filtrate for later use.

Described seed of Job's tears are the seed of Job's tears below 8wt% by dried water content, are placed in parch pot in 120～150 ℃ of parches 20～55 minutes, until surperficial yellowish-brown takes out for subsequent use; Described Semen Fagopyri Esculenti is the seed of Job's tears below 8wt% by dried water content, is placed in parch pot in 120～150 ℃ of parches 20～55 minutes, until surperficial yellowish-brown takes out for subsequent use; Described Herba Verbenae comprises Herba Verbenae stem and leaf, flower and the seed after cleaning up and drying.

4. a Chinese medicine composition for prevention, treatment diabetes, is characterized in that described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar; 5～10 parts of supplementary elements, described supplementary element is Lumbrukinase.

5. a Chinese medicine composition for prevention, treatment diabetes, is characterized in that described formula is by following weight composition: 15 parts of Herba Verbenae, 1.5 parts of Herba Typhonii gigantei, 15 parts of Semen Coicis roots, 15 parts of Rhizoma Coptidis, 15 parts of Auricularia, 15 parts, Tremella, 15 parts, Oryza glutinosa, 15 parts of seed of Job's tears, 15 parts, Semen Fagopyri Esculenti, 28 parts of Mel, 75 parts of vinegar; 5～10 parts of supplementary elements, described supplementary element is the mixture of Chinese crude drug Radix Tylophorae arenicolae, Chinese crude drug leaf of Cyclocarya paliurus Iljinskaja, Chinese crude drug Conocephalum conicum(L．) Dumortier．, weight mixed proportion is 1:1～2:1.

6. the Chinese medicine composition of a kind of prevention according to claim 5, treatment diabetes, is characterized in that: described Lumbrukinase is prepared by following steps:

7. the Chinese medicine composition of a kind of prevention according to claim 5, treatment diabetes, is characterized in that: it is used to, and preparation is treated or the medicament of prevent diabetes.

8. the Chinese medicine composition of a kind of prevention according to claim 5, treatment diabetes, is characterized in that: it is used to, and preparation is treated or food or the health product of prevent diabetes.

9. according to the Chinese medicine composition of a kind of prevention described in claim 1-5, treatment diabetes, it is characterized in that: it is used to, and preparation is treated or the medicament of prevent diabetes complication.

10. the preparation method of the Chinese medicine composition of a kind of prevention according to claim 2, treatment diabetes, is characterized in that step is as follows:

Step 1,1) select commercially available super micron mill;