CN104258193B - A kind of pharmaceutical composition for treating neurasthenia and prevention senile dementia - Google Patents
A kind of pharmaceutical composition for treating neurasthenia and prevention senile dementia Download PDFInfo
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Abstract
The present invention discloses a kind of pharmaceutical composition for treating neurasthenia and prevention senile dementia, is made up of the component of following masses percentage：Malania oleifera nervonic acid 1~10%, helicidum 1~10%, coix seed lactone 1~10%, surplus are auxiliary material.The medicine that the present invention is provided contributes to the extension of the length of one's sleep of the elderly, coix seed lactone, which can give protection against cancer, suppresses a variety of cancer cell multiplications, three, which shares, effectively can prevent and control the generation and healing of senile dementia, and a kind of safely and effectively plant composition of pure plant extraction is brought in the whole world increasingly severe to aging.The prescription compatibility that the present invention is used is reasonable, raw material is all from natural plants, while the preparation method for the Chinese medicine composition that the present invention is provided, it is workable, it is related to extraction process especially in accordance with each taste effective component of chinese medicine chemical property, extract obtained active constituent content is high, has the advantages that drug effect height, quantity are few, and process stabilizing, it is adapted to the big production of industry.
Description
Technical field
The invention belongs to biomedicine technical field, it is related to a kind of medicine for treating neurasthenia and prevention senile dementia
Composition.
Background technology
With the implementation of China's expanding economy, and population policy, the age composition of China human mortality there occurs very big
Transformation.The life expectancy of China rose to 73.8 years old in 2008 from 68.4 years old of nineteen ninety, and elderly population account for the ratio of total population
Increasing, the data provided according to the Organization of Economy and Cooperation Development of weight, are estimated to exceed 65 with Chinese total population comparatively
The population ratio in year will rise to the 16.3% of the year two thousand thirty from 7.8% in 2005.
It was discovered by researchers that after the brain tissue of shark is inflicted heavy losses on, energy auto restore facility, because in the brain tissue of shark
The material of " nervonic acid " is containing one kind, cerebral nerve signaling pathway can be repaired --- nerve fibre, and promote neural thin
Born of the same parents regenerate.Nowadays, nervonic acid（NA）It is cerebral nerve fiber and nerve cell to be known as always by the scientist of various countries
Core natural constituent.Nervonic acid can also effectively treat senile dementia, brain paralysis, encephalatrophy, memory loss, insomnia forgetfulness
Deng brain diseases.And the mankind are different with shark, mankind itself can not synthesize nervonic acid, with age or brain is created
Wound, is likely to cause the decay of nervonic acid, causes disease.
Nervonic acid is extracted from shark must kill shark, destruction marine ecology balance, as international organization forbids catching and killing
The cry more and more higher of shark, the country such as America and Europe all attaches great importance to excavate nervonic acid resource from plant, it has been investigated that, contain
The plant of more than 2% nervonic acid has more than ten kinds, wherein with Chinese peculiar single plant --- neural acid content highest in malania oleifera,
And up to 62.6%, its Development volue and economic value are huge, it can be described as " golden fruit in plant ".
Malania oleifera (Malania oleiferaChun et Lee) it is Olacaceae(Olacaceae), malania oleifera category(Malania)Plant.Also known as mountain paulownia fruit, after kalimeris.Belong to Chinese Second Class Key Protected Plant.It is the southeast such as Funing in Yunnan, wide
South, mountain of papers are distributed.The benevolence of malania oleifera, rich in abundant grease, is musk ambrette ketone(muscine)Desirable feedstock.Garlic
Head fruit seed oil content is up to 50~60%, and the aliphatic acid after saponification contains 40~50% tetracosa carbon -15- olefin(e) acids, i.e., refreshing
Through acid, it helps to recover nerve endings activity, promotes nerve growth and development, there is anti-senile dementia.
According to statistics, there are 24,000,000 dementia patients in the world now.In China, the number of patients of senile dementia is up to more than 500
Ten thousand, and also have 300,000 new cases every year.The pathogenesis and new drug development of senile dementia are just by countries in the world
Great attention.Therefore, using malania oleifera as raw material, edible oil, health products and the medicine of exploitation treatment senile dementia have extensively
Big market potential and medical value.
Coix seed(Coix seed), be grass family herbaceos perennial Job's tears (Coix lacryma-jobiL.)
Ripe kernel, coix seed is produced in China's most area, because the nutritive value of Job's tears is very high, is described as world's grass
The king of section plant.Coix seed contains about 2.7% or so coix seed oil, and intelligent seed of jog's tears benevolence is containing protein 16.2%, and fatty 4.65%,
Carbohydrate 79.17%, a small amount of vitamin B1.Coix seed contains several amino acids（Including leucine, lysine, arginine, junket
Propylhomoserin etc.）Also containing materials such as Coixol, Coixenolide, triterpenoids.Suppress having for kinds of tumor cells propagation in coix seed
Effect composition is Coixenolide, coix seed lactone, and such compound also has enhancing hormone control function and promotes immune system and enzyme
The function of system, also there is facilitation to cellular immunity and humoral immunity, and a large amount of zooperies and clinical confirmation coix seed oil have
Strengthen immunity and antitumor action.Adlay has plantation in China various regions, and Yunnan Province is using Qujing Shizong County as representative, adlay
Resource is relatively enriched, and is one of important adlay life area of China.Coix seed is nutritious, and with antitumor, living well
The medical values such as blood stagnation resolvation, in the market has occurred in that some coix seed products, adlay health liquor, adlay vinegar, adlay one by one
Powder, adlay tea etc., are welcome by consumers in general very much.But compared with the product of external adlay, domestic adlay product development phase
To deficiency, the utilization of adlay is also rested in the diet of people substantially, deep processed product famine.
Bean curd, nickname sow fruit, bean curd berry extract is bean curd former times（helicid）, PARA FORMALDEHYDE PRILLS(91,95) benzene-O- β-D- Ahs cough up
Pyranoside, also known as Helicid, are Yunnan Wild plant Proteaceae radish tree(Helieiaerra6c:Hook.f)'s
Fruit（It is commonly called as nilagiri helicia root and leaf）, its chemical constitution class is similar to Gastrodin.Pharmacology and clinical research show that bean curd former times is to maincenter
The effect of nervous system is similar to Gastrodin, but its calm, analgesic effect is strong compared with Gastrodin, to head caused by neurosis
Bitterly, dizzy, the therapeutic action markedly fast of sleep-disorder, while having to memory, thinking ability preferably improves and adjustment effect, should
With having a extensive future.Sow fruit is mainly distributed on Yunnan Province south and southwestern some areas, most with Puer City and Honghe distribution,
It is most of to be in wild state, it would be highly desirable to which that biological engineering protects the exploitation with deep processed product.
The present invention using three kinds in Yunnan Province's integrated distribution or the medicinal plant of cultivation as research object, carried using Phytochemistry
Take means to extract prevention senile dementia, antidepression and antineoplastic effective composition from these three plants respectively, and use medicine
Agent learns to do section and is prepared into Chinese medicine health-care tablet or capsule, and a kind of safely and effectively traditional Chinese medicine health care product is provided for middle-older patient.
The content of the invention
To solve to prevent the raw material of senile dementia, antidepression and antitumor medicine to be difficult to obtain and mesh in the prior art
Preceding medicine has that the present invention provides a kind of drug regimen for treating neurasthenia and prevention senile dementia
Thing, to prepare anti-senile dementia, neurasthenia, anti-tumor drug using pure plant raw material.
The present invention is realized by following technical proposal：A kind of drug regimen for treating neurasthenia and prevention senile dementia
Thing, it is characterised in that be made up of the component of following masses percentage：
Malania oleifera nervonic acid 1~10%,
Helicidum 1~10%,
Coix seed lactone 1~10%,
Surplus is auxiliary material.
The auxiliary material includes the conventional dextrin of pharmaceutical field, lactose, sodium carboxymethyl starch, microcrystalline cellulose, starch, hydroxypropyl
Base cellulose.
The malania oleifera nervonic acid is followed these steps to be extracted, purified：
（1）It is 1~10 by solid-to-liquid ratio g/mL by malania oleifera seed decapsidate and epidermis:5~50, with 3~4 DEG C of concentration
For 20mM, pH=6.0~7.0 phosphate buffer smashed to pieces after, then through ultrasonic grind 30~50min is handled, in 3~4
It is stripped overnight, then filters at DEG C, then with 5000~8000 r/min rotating speed 30 is centrifuged at 3~4 DEG C
~40 min, are repeated twice, and remove supernatant and obtain residue；
（2）By step（1）Gained residue presses solid-to-liquid ratio g/mL=1~5:5~20 addition petroleum ethers are stirred, then quiet
Layering is put, filtrate is collected after filtering, then soaks with filtrate filter residue repeatedly 2-3 times, soaks filtered after 30~60min every time, close
And filtrate, then rotary evaporation is carried out to remove petroleum ether at 40~50 DEG C, obtain Malania Oleifera Oil；
（3）By applied sample amount:Silica gel amount=1~10:10~100 mass ratio weighs silica G, first by solid-to-liquid ratio g/mL=1~
5:2~10 add petroleum ether dissolution silica G, are stirred for after 20~30min, are poured into chromatographic column, treat silica gel column chromatography not
Containing bubble, after settlement stability, by step（2）Gained Malania Oleifera Oil presses solid-to-liquid ratio g/mL=1~5:1~10 addition petroleum ether is molten
Xie Hou, loads silica gel column chromatography upper strata, and gradient elution is carried out using petroleum ether-ethyl acetate elution system, by being dissolved with garlic
The petroleum ether of fruit oil:Ethyl acetate=50:1~30:1（V/V）Cut, cut is concentrated under reduced pressure in less than 40~50 DEG C,
Obtain colourless lump shaped crystalline, as malania oleifera nervonic acid.
With thin-layered chromatography by nervonic acid standard items and colourless crystallization point sample on same lamellae, expansion is compared
Right, as a result the colourless crystallization and nervonic acid standard items show identical purple spot in the same position of same lamellae, and
Same color spot and identical Rf values are respectively provided with three kinds of different development systems, it was demonstrated that the colourless crystallization is nervonic acid.This side
The neural acid content that method is obtained is more than 95%.
The step（1）Phosphate buffer by it is following operation prepare：By the disodium phosphate soln that concentration is 0.2M
It is that 0.2M sodium dihydrogen phosphates are 12.3~61 by volume with concentration:87.7~39 mixing, that is, obtain pH=6.0~7.0
Phosphate buffer.
The step（1）Supernatant through the isolated protein precipitation of subsequent purification.
The step（3）Silica G granularity be 200~300 mesh.
Helicidum extraction purification as follows, by following each step：
（1）Bean curd seed crush, cross 100 mesh sieves, add volumetric concentration be 50~70% ethanol at 20~30 DEG C
30~60min of cold soaking, then 30~60min of ultrasonic wave extraction, filtering, filtrate carry out rotary evaporation concentration at 45~55 DEG C, return
Ethanol is received, sow fruit ethanol extract is obtained；
（2）In step（1）It is 1~5 that solid-to-liquid ratio g/mL is pressed in gained bean curd ethanol extract:5~25 addition pure water enter
Row dissolving, upper macroreticular resin D101 types first remove the water-solubility impurities such as polysaccharide, protein, then use volumetric concentration with pure water elution
For 30~50% 4~5 retention volumes of ethanol elution, the ethanol efflux that macroreticular resin volumetric concentration is 30~50% is collected,
Through being concentrated under reduced pressure, the concentration for adding 2~3 times of concentrate volume is 90~95% ethanol, and 12~24h is stood at 3~4 DEG C
To separate out crystallization, then suction filtration goes out crystallization, that is, obtains helicidum sterling.Gained helicidum purity is more than 95%.
Coix seed lactone extraction purification as follows, by following each step：
Coix seed is crushed, 100 mesh sieves are crossed, then by coix seed powder in 35~40 DEG C of temperature, 37~40MPa of pressure, CO2Stream
Measure under conditions of 10~20kg/h carry out 1~2h of supercritical carbon dioxide extracting, use volumetric concentration for 70~95% ethanol
Dissolution extraction material, then rotated evaporative removal ethanol, obtain the grease of yellow, as coix seed lactone.
Above-mentioned malania oleifera nervonic acid, helicidum, coix seed lactone extract are sufficiently mixed in proportion, are added pharmaceutically
Acceptable auxiliary material is prepared into any conventional oral formulations, such as capsule, granule, oral liquid, pill or tablet.
Advantage and effect that the present invention possesses：The medicine that the present invention is provided is mainly the small molecule chemical combination such as aliphatic acid, lactone
The assembly of thing, means are extracted by Phytochemistry, extracted from the several plant that Yunnan is peculiar and large area is planted effectively into
Point, wherein nervonic acid has an effect of significant anti-senile dementia disease, and helicidum can releive old dementia patients anxiety and tense
Mood, contribute to the extension of the length of one's sleep of the elderly, coix seed lactone can give protection against cancer a variety of cancer cell multiplications of suppression, San Zhehe
With that effectively can prevent and control the generation and healing of senile dementia, the whole world increasingly severe to aging is brought a kind of pure
The safely and effectively plant composition of plant extraction.Rationally, raw material is all from natural plants to the prescription compatibility that the present invention is used, together
When the preparation method of Chinese medicine composition that provides of the present invention, it is workable, especially in accordance with each taste effective component of chinese medicine chemistry
Property is related to extraction process, and extract obtained active constituent content is high, has the advantages that drug effect height, quantity are few, and technique
It is stable, it is adapted to the big production of industry.
Embodiment
Further illustrate the present invention by the following examples.
Embodiment 1
The pharmaceutical composition of neurasthenia and prevention senile dementia is treated, is made up of the component of following masses percentage：
Malania oleifera nervonic acid 2%,
Helicidum 6%,
Coix seed lactone 5%,
Auxiliary material 87%,
Wherein auxiliary material is dextrin, lactose, sodium carboxymethyl starch, microcrystalline cellulose, starch.
Wherein malania oleifera nervonic acid is followed these steps to be extracted, purified：
（1）It is 10 by solid-to-liquid ratio g/mL by malania oleifera seed decapsidate and epidermis:50, with 4 DEG C of concentration be 20mM, pH=
After 6.0 phosphate buffer is smashed to pieces, then through ultrasonic grind handle 40min, in being stripped overnight at 3 DEG C, then
40 min are centrifuged with 6000 r/min rotating speed with 4 layers of filtered through gauze, then at 3 DEG C, is repeated twice, removes supernatant
Liquid obtains residue；Wherein phosphate buffer is prepared by following operation：By disodium phosphate soln and concentration of the concentration for 0.2M
It is 61 by volume for 0.2M sodium dihydrogen phosphates:39 mixing, that is, obtain the phosphate buffer of pH=7.0；Supernatant is after
Continuous purifies and separates obtain protein precipitation；
（2）By step（1）Gained residue presses solid-to-liquid ratio g/mL=3:20 addition petroleum ethers are stirred, then stratification,
Filtrate is collected after filtering, then soaks with filtrate filter residue repeatedly 3 times, is filtered every time after immersion 50min, merging filtrate, then 40
Rotary evaporation is carried out at DEG C to remove petroleum ether, flaxen Malania Oleifera Oil is obtained；
（3）By applied sample amount:Silica gel amount=5:60 mass ratio weigh granularity be 300 mesh silica G, first by solid-to-liquid ratio g/mL=
3:8 add petroleum ether dissolution silica G, are stirred for after 25min, are poured into chromatographic column, treat that silica gel column chromatography does not contain bubble,
After settlement stability, by step（2）Gained Malania Oleifera Oil presses solid-to-liquid ratio g/mL=5:10 add after petroleum ether dissolution, load layer of silica gel
Post upper strata is analysed, gradient elution is carried out using petroleum ether-ethyl acetate elution system, by the petroleum ether for being dissolved with Malania Oleifera Oil:Second
Acetoacetic ester=40:1（V/V）Cut, cut is concentrated under reduced pressure in less than 50 DEG C, colourless lump shaped crystalline, as garlic is obtained
Fruit nervonic acid.
With thin-layered chromatography by nervonic acid standard items and colourless crystallization point sample on same lamellae, expansion is compared
Right, as a result the colourless crystallization and nervonic acid standard items show identical purple spot in the same position of same lamellae, and
Same color spot and identical Rf values are respectively provided with three kinds of different development systems, it was demonstrated that the colourless crystallization is nervonic acid.This side
The neural acid content that method is obtained is more than 95%.Nervonic acid yield is the 10.462% of Malania Oleifera Oil.
Wherein helicidum extraction purification as follows, by following each step：
（1）Bean curd seed crush, cross 100 mesh sieves, add volumetric concentration be 70% ethanol at 25 DEG C cold soaking
40min, then ultrasonic wave extraction 60min, filtering, filtrate carry out rotary evaporation concentration at 45 DEG C, reclaim ethanol, obtain sow fruit
Ethanol extract；
（2）In step（1）It is 5 that solid-to-liquid ratio g/mL is pressed in gained bean curd ethanol extract:25 addition pure water are dissolved,
Upper macroreticular resin D101 types, first remove polysaccharide, the water-solubility impurity such as protein with pure water elution, then with volumetric concentration are 50%
5 retention volumes of ethanol elution, collect the ethanol efflux that macroreticular resin volumetric concentration is 30%, through being concentrated under reduced pressure, add dense
The ethanol that the concentration that contracting object accumulates 3 times is 92%, stands 20h to separate out crystallization at 3 DEG C, then suction filtration goes out crystallization, that is, obtains bean curd
Fruit glycosides sterling.Gained helicidum purity is more than 95%, and the yield of helicidum is the 1.06% of sow fruit seed.
Wherein coix seed lactone extraction purification as follows, by following each step：
Coix seed is crushed, 100 mesh sieves are crossed, then by coix seed powder in 40 DEG C of temperature, pressure 37MPa, CO2Flow is
Under conditions of 15kg/h carry out supercritical carbon dioxide extracting 1h, use volumetric concentration for 70% ethanol dissolution extraction material, then
Rotated evaporative removal ethanol, obtains the grease of yellow, as coix seed lactone.Gained coix seed lactone extracts yield
5.3%。
Above-mentioned malania oleifera nervonic acid, helicidum, coix seed lactone extract are sufficiently mixed in proportion, are added pharmaceutically
Acceptable auxiliary material is prepared into any conventional capsule.
Embodiment 2
The pharmaceutical composition of neurasthenia and prevention senile dementia is treated, is made up of the component of following masses percentage：
Malania oleifera nervonic acid 1%,
Helicidum 10%,
Coix seed lactone 10%,
Auxiliary material 79%,
Wherein auxiliary material is dextrin, lactose, sodium carboxymethyl starch, microcrystalline cellulose, starch, hydroxypropyl cellulose.
Wherein malania oleifera nervonic acid is followed these steps to be extracted, purified：
（1）It is 1 by solid-to-liquid ratio g/mL by malania oleifera seed decapsidate and epidermis:30, with 3 DEG C of concentration be 20mM, pH=
After 7.0 phosphate buffer is smashed to pieces, then through ultrasonic grind handle 50min, in being stripped overnight at 4 DEG C, then
35min is centrifuged with 5000r/min rotating speed with 4 layers of filtered through gauze, then at 4 DEG C, is repeated twice, removes supernatant
Obtain residue；Wherein phosphate buffer is prepared by following operation：The disodium phosphate soln for being 0.2M by concentration with concentration is
0.2M sodium dihydrogen phosphates are 12.3 by volume:87.7 mixing, that is, obtain the phosphate buffer of pH=6.0；Supernatant is passed through
The isolated protein precipitation of subsequent purification；
（2）By step（1）Gained residue presses solid-to-liquid ratio g/mL=5:10 addition petroleum ethers are stirred, then stratification,
Filtrate is collected after filtering, then soaks with filtrate filter residue repeatedly 2 times, is filtered every time after immersion 30min, merging filtrate, then 45
Rotary evaporation is carried out at DEG C to remove petroleum ether, flaxen Malania Oleifera Oil is obtained；
（3）By applied sample amount:Silica gel amount=10:100 mass ratio weighs the silica G that granularity is 250 mesh, first by solid-to-liquid ratio g/
mL=5:2 add petroleum ether dissolution silica G, are stirred for after 20min, are poured into chromatographic column, treat that silica gel column chromatography does not contain gas
After bubble, settlement stability, by step（2）Gained Malania Oleifera Oil presses solid-to-liquid ratio g/mL=3:1 adds after petroleum ether dissolution, loads silica gel
Chromatographic column upper strata, carries out gradient elution, by the petroleum ether for being dissolved with Malania Oleifera Oil using petroleum ether-ethyl acetate elution system:
Ethyl acetate=50:1（V/V）Cut, cut is concentrated under reduced pressure in less than 45 DEG C, colourless lump shaped crystalline, as garlic is obtained
Head fruit nervonic acid.
With thin-layered chromatography by nervonic acid standard items and colourless crystallization point sample on same lamellae, expansion is compared
Right, as a result the colourless crystallization and nervonic acid standard items show identical purple spot in the same position of same lamellae, and
Same color spot and identical Rf values are respectively provided with three kinds of different development systems, it was demonstrated that the colourless crystallization is nervonic acid.This side
The neural acid content that method is obtained is more than 95%.Nervonic acid yield is the 11.054% of Malania Oleifera Oil.
Wherein helicidum extraction purification as follows, by following each step：
（1）Bean curd seed crush, cross 100 mesh sieves, add volumetric concentration be 60% ethanol at 20 DEG C cold soaking
60min, then ultrasonic wave extraction 45min, filtering, filtrate carry out rotary evaporation concentration at 50 DEG C, reclaim ethanol, obtain sow fruit
Ethanol extract；
（2）In step（1）It is 3 that solid-to-liquid ratio g/mL is pressed in gained bean curd ethanol extract:19 addition pure water are dissolved,
Upper macroreticular resin D101 types, first remove polysaccharide, the water-solubility impurity such as protein with pure water elution, then with volumetric concentration are 40%
4 retention volumes of ethanol elution, collect the ethanol efflux that macroreticular resin volumetric concentration is 40%, through being concentrated under reduced pressure, add dense
The ethanol that the concentration that contracting object accumulates 2 times is 95%, stands 24h to separate out crystallization at 4 DEG C, then suction filtration goes out crystallization, that is, obtains bean curd
Fruit glycosides sterling.Gained helicidum purity is more than 95%, and the yield of helicidum is the 1.57% of sow fruit seed.
Wherein coix seed lactone extraction purification as follows, by following each step：
Coix seed is crushed, 100 mesh sieves are crossed, then by coix seed powder in 35 DEG C of temperature, pressure 40MPa, CO2Flow is
Under conditions of 10kg/h carry out supercritical carbon dioxide extracting 2h, use volumetric concentration for 85% ethanol dissolution extraction material, then
Rotated evaporative removal ethanol, obtains the grease of yellow, as coix seed lactone.Gained coix seed lactone extracts yield
5.9%。
Above-mentioned malania oleifera nervonic acid, helicidum, coix seed lactone extract are sufficiently mixed in proportion, are added pharmaceutically
Acceptable auxiliary material is prepared into any conventional granule, pill or tablet.
Embodiment 3
The pharmaceutical composition of neurasthenia and prevention senile dementia is treated, is made up of the component of following masses percentage：
Malania oleifera nervonic acid 10%,
Helicidum 1%,
Coix seed lactone 1%,
Auxiliary material 88%,
Wherein auxiliary material is dextrin, lactose, sodium carboxymethyl starch, microcrystalline cellulose, starch, hydroxypropyl cellulose.
Wherein malania oleifera nervonic acid is followed these steps to be extracted, purified：
（1）It is 8 by solid-to-liquid ratio g/mL by malania oleifera seed decapsidate and epidermis:5, with 4 DEG C of concentration be 20mM, pH=
After 7.0 phosphate buffer is smashed to pieces, then through ultrasonic grind handle 30min, in being stripped overnight at 4 DEG C, then
30min is centrifuged with 8000 r/min rotating speed with 4 layers of filtered through gauze, then at 4 DEG C, is repeated twice, removes supernatant
Liquid obtains residue；Wherein phosphate buffer is prepared by following operation：By disodium phosphate soln and concentration of the concentration for 0.2M
It is 46 by volume for 0.2M sodium dihydrogen phosphates:68 mixing, that is, obtain the phosphate buffer of pH=7.0；Supernatant is after
Continuous purifies and separates obtain protein precipitation；
（2）By step（1）Gained residue presses solid-to-liquid ratio g/mL=1:5 addition petroleum ethers are stirred, then stratification,
Filtrate is collected after filtering, then soaks with filtrate filter residue repeatedly 3 times, is filtered every time after immersion 60min, merging filtrate, then 50
Rotary evaporation is carried out at DEG C to remove petroleum ether, flaxen Malania Oleifera Oil is obtained；
（3）By applied sample amount:Silica gel amount=1:10 mass ratio weigh granularity be 200 mesh silica G, first by solid-to-liquid ratio g/mL=
1:10 add petroleum ether dissolution silica G, are stirred for after 30min, are poured into chromatographic column, treat that silica gel column chromatography does not contain gas
After bubble, settlement stability, by step（2）Gained Malania Oleifera Oil presses solid-to-liquid ratio g/mL=1:8 add after petroleum ether dissolution, load silica gel
Chromatographic column upper strata, carries out gradient elution, by the petroleum ether for being dissolved with Malania Oleifera Oil using petroleum ether-ethyl acetate elution system:
Ethyl acetate=30:1（V/V）Cut, cut is concentrated under reduced pressure in less than 40 DEG C, colourless lump shaped crystalline, as garlic is obtained
Head fruit nervonic acid.
With thin-layered chromatography by nervonic acid standard items and colourless crystallization point sample on same lamellae, expansion is compared
Right, as a result the colourless crystallization and nervonic acid standard items show identical purple spot in the same position of same lamellae, and
Same color spot and identical Rf values are respectively provided with three kinds of different development systems, it was demonstrated that the colourless crystallization is nervonic acid.This side
The neural acid content that method is obtained is more than 95%.Nervonic acid yield is the 10.819% of Malania Oleifera Oil.
Wherein helicidum extraction purification as follows, by following each step：
（1）Bean curd seed crush, cross 100 mesh sieves, add volumetric concentration be 50% ethanol at 30 DEG C cold soaking
30min, then ultrasonic wave extraction 30min, filtering, filtrate carry out rotary evaporation concentration at 55 DEG C, reclaim ethanol, obtain sow fruit
Ethanol extract；
（2）In step（1）It is 1 that solid-to-liquid ratio g/mL is pressed in gained bean curd ethanol extract:5 addition pure water are dissolved,
Upper macroreticular resin D101 types, first remove polysaccharide, the water-solubility impurity such as protein with pure water elution, then with volumetric concentration are 30%
4 retention volumes of ethanol elution, collect the ethanol efflux that macroreticular resin volumetric concentration is 50%, through being concentrated under reduced pressure, add dense
The ethanol that the concentration that contracting object accumulates 2 times is 90%, stands 12h to separate out crystallization at 4 DEG C, then suction filtration goes out crystallization, that is, obtains bean curd
Fruit glycosides sterling.Gained helicidum purity is more than 95%, and the yield of helicidum is the 1.13% of sow fruit seed.
Wherein coix seed lactone extraction purification as follows, by following each step：
Coix seed is crushed, 100 mesh sieves are crossed, then by coix seed powder in 38 DEG C of temperature, pressure 38MPa, CO2Flow is
Under conditions of 20kg/h carry out supercritical carbon dioxide extracting 1.5h, use volumetric concentration for 95% ethanol dissolution extraction material,
Rotated evaporative removal ethanol, obtains the grease of yellow, as coix seed lactone again.Gained coix seed lactone extracts yield
5.77%。
Above-mentioned malania oleifera nervonic acid, helicidum, coix seed lactone extract are sufficiently mixed in proportion, are added pharmaceutically
Acceptable auxiliary material is prepared into any conventional oral liquid.
The pharmaceutical composition for the treatment of neurasthenia obtained by above-described embodiment and prevention senile dementia is taken, for following clinical
Treatment：
First, treatment target
30 of neurastheniac；30 of patients of senile dementia；50 of patients with depression.
Above-mentioned all kinds of cases are bisected into treatment group and each 55 of control group at random.
2nd, usage
The pharmaceutical composition for the treatment of neurasthenia obtained by above-described embodiment and prevention senile dementia is taken by treatment group, once
2g, 1 times a day, even served 30 and is observed.
Take god purchased in market in control group to decline recovering capsule, one is for several times, one time 5,2 times a day, even served 30 and observed.
3rd, result
Treatment neurasthenia and the pharmaceutical composition clinical therapeutic efficacy of prevention senile dementia that the present invention is provided：It is effective
Rate is efficient to neurastheniac's symptom up to 83% up to 100%；It is efficient to patients of senile dementia symptom up to 78%；To depression
Disease patient symptom is efficient up to 90%.
And control group is efficient to neurastheniac's symptom up to 75%；Patients of senile dementia symptom effective percentage is reached
65%；It is efficient to patients with depression symptom up to 43%.
4th, conclusion
The pharmaceutical composition for the treatment of neurasthenia that the present invention is provided and prevention senile dementia be by malania oleifera nervonic acid,
The composition of the compositions such as helicidum, coix seed lactone extract, to anti-senile dementia, neurasthenia, antitumor has notable treatment
Effect, it, which is acted on, is better than control group.
The pharmaceutical composition full utilization plant extract purifying of the treatment neurasthenia and prevention senile dementia, raw material is easy
Obtain, it is effective in cure it is good, using safety, toxic side effect is small the advantages of, obtained product quality is stable, easy to use, is more suitable for
It is without any side effects in clinical application, it is evident in efficacy.
Claims (4)
1. a kind of pharmaceutical composition for treating neurasthenia and prevention senile dementia, it is characterised in that by following masses percentage
Component composition：
Malania oleifera nervonic acid 1~10%,
Helicidum 1~10%,
Coix seed lactone 1~10%,
Surplus is auxiliary material；
It is fine that the auxiliary material includes the conventional dextrin of pharmaceutical field, lactose, sodium carboxymethyl starch, microcrystalline cellulose, starch, hydroxypropyl
Dimension element；
Wherein, the malania oleifera nervonic acid is followed these steps to be extracted, purified：
（1）It is 1~10 by solid-to-liquid ratio g/mL by malania oleifera seed decapsidate and epidermis:5~50, the concentration with 3~4 DEG C is
After 20mM, pH=6.0~7.0 phosphate buffer are smashed to pieces, then through ultrasonic grind 30~50min is handled, in 3~4 DEG C
Under be stripped overnight, then filter, then be centrifuged 30 at 3~4 DEG C with 5000~8000 r/min rotating speed~
40 min, are repeated twice, and remove supernatant and obtain residue；
（2）By step（1）Gained residue presses solid-to-liquid ratio g/mL=1~5:5~20 addition petroleum ethers are stirred, then are stood point
Layer, collects filtrate after filtering, then soaks with filtrate filter residue repeatedly 2~3 times, soaks filtered after 30~60min every time, merge filter
Liquid, then rotary evaporation is carried out to remove petroleum ether at 40~50 DEG C, obtain Malania Oleifera Oil；
（3）By applied sample amount:Silica gel amount=1~10:10~100 mass ratio weighs silica G, first by solid-to-liquid ratio g/mL=1~5:2~
10 add petroleum ether dissolution silica G, are stirred for after 20~30min, are poured into chromatographic column, treat that silica gel column chromatography does not contain gas
After bubble, settlement stability, by step（2）Gained Malania Oleifera Oil presses solid-to-liquid ratio g/mL=1~5:1~10 adds after petroleum ether dissolution, dress
Enter silica gel column chromatography upper strata, gradient elution is carried out using petroleum ether-ethyl acetate elution system, by the stone for being dissolved with Malania Oleifera Oil
Oily ether:Ethyl acetate=50:1~30:1（V/V）Cut, cut is concentrated under reduced pressure in less than 40~50 DEG C, obtains colourless
Lump shaped crystalline, as malania oleifera nervonic acid；
Helicidum extraction purification as follows, by following each step：
（1）Bean curd seed crush, cross 100 mesh sieves, add volumetric concentration be 50~70% ethanol at 20~30 DEG C cold soaking
30~60min, then 30~60min of ultrasonic wave extraction, filtering, filtrate carry out rotary evaporation concentration at 45~55 DEG C, reclaim second
Alcohol, obtains sow fruit ethanol extract；
（2）In step（1）It is 1~5 that solid-to-liquid ratio g/mL is pressed in gained bean curd ethanol extract:5~25 addition pure water carry out molten
Solution, upper macroreticular resin D101 types first remove polysaccharide, the water-solubility impurity such as protein with pure water elution, then with volumetric concentration are 30
~50% 4~5 retention volumes of ethanol elution, collect the ethanol efflux that macroreticular resin volumetric concentration is 30~50%, through subtracting
Pressure concentration, the concentration for adding 2~3 times of concentrate volume is 90~95% ethanol, and 12~24h is stood at 3~4 DEG C to analyse
Go out crystallization, then suction filtration goes out crystallization, that is, obtains helicidum sterling；
Coix seed lactone extraction purification as follows；
Coix seed is crushed, 100 mesh sieves are crossed, then by coix seed powder in 35~40 DEG C of temperature, 37~40MPa of pressure, CO2Flow is
1~2h of supercritical carbon dioxide extracting is carried out under conditions of 10~20kg/h, uses volumetric concentration to be dissolved for 70~95% ethanol
Extracting substance, then rotated evaporative removal ethanol, obtain the grease of yellow, as coix seed lactone.
2. pharmaceutical composition according to claim 1, it is characterised in that：The step（1）Phosphate buffer press
Row operation is prepared：The disodium phosphate soln for being 0.2M by concentration with concentration is that 0.2M sodium dihydrogen phosphates are by volume
12.3~61:87.7~39 mixing, that is, obtain the phosphate buffer of pH=6.0~7.0.
3. pharmaceutical composition according to claim 1, it is characterised in that：The step（1）Supernatant through subsequent purification
Isolated protein precipitation.
4. pharmaceutical composition according to claim 1, it is characterised in that：The step（3）Silica G granularity be 200
~300 mesh.
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| CN105037448B (en) * | 2015-09-10 | 2017-09-15 | 福建农林大学 | A kind of method that rapid, high volume extracts the separation oligomeric sugar monomer of coix seed |
| CN105853996A (en) * | 2016-03-31 | 2016-08-17 | 山东健科生物技术有限公司 | A cranial nerve activating and restoring liquid |
| CN106376933A (en) * | 2016-08-30 | 2017-02-08 | 昆明酷特利生物科技有限公司 | Brain-invigorating antioxidant health product and applications thereof |
| CN107594268A (en) * | 2017-08-04 | 2018-01-19 | 杨宇明 | A kind of invigorating brain and relieving mental uneasiness nervonic acid compound beverage antianxity and preparation method thereof |
| CN107875369A (en) * | 2017-12-19 | 2018-04-06 | 北京昆达中医医学研究院 | A kind of Chinese medicine natural drug for treating senile dementia |
| CN108467345B (en) * | 2018-04-10 | 2021-03-09 | 大理大学 | Method for extracting nervonic acid from garlic fruits and nervonic acid clathrate compound |
| CN111117773B (en) * | 2019-12-25 | 2022-12-09 | 昆明酷特利生物科技有限公司 | Method for separating nervonic acid from garlic oil and application thereof |
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| CN102824353A (en) * | 2011-06-14 | 2012-12-19 | 上海现代药物制剂工程研究中心有限公司 | Helicid oral preparation, and preparation method and application thereof |
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