CN101683396A - Extraction method of tropaeolum total lavonoids - Google Patents
Extraction method of tropaeolum total lavonoids Download PDFInfo
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- CN101683396A CN101683396A CN200810166951A CN200810166951A CN101683396A CN 101683396 A CN101683396 A CN 101683396A CN 200810166951 A CN200810166951 A CN 200810166951A CN 200810166951 A CN200810166951 A CN 200810166951A CN 101683396 A CN101683396 A CN 101683396A
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- tropaeolum
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- lavonoids
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Abstract
The invention relates to an extraction method of tropaeolum total lavonoids. The method is mainly characterized by comprising the following steps: (1) drying tropaeolum stems and leaves (or tropaeolum) until a water content is below 8 percent; (2) crushing into fine powder above 300 meshes by ultrafine powder crushing equipment; (3) adding water with a pH value of 7-8 and stirring and leaching at50 DEG C; (4) centrifugating, filtering and deslagging; (5) absorbing the liquid medicine by macroporous resin; (6) eluting the resin by 70 percent of ethanol; (7) recycling the ethanol; and (8) spraying and drying to obtain the tropaeolum total lavonoids with dry extracts of over 50 percent. The invention adopts ultrafine powder extraction and leaches crushed plant cells at a low temperature. Compared with a traditional method, the invention has low extraction temperature, short extraction time and high extraction rate of effective contents, not only improves the yield of the effective flowercontents, but also effectively utilizes the originally discarded tropaeolum stems and leaves to greatly improve the utilization rate of resources. Determined by experiments, the process improves theextraction rate of the tropaeolum total lavonoids than a traditional decocting process by 1.2 times and greatly improves the product purity.
Description
Technical field:
The present invention relates to a kind of extraction and separation method of total flavones of tropaeolum.
Background technology:
Flos Trollii is a ranunculaceae plant, the dry flower of Flos Trollii Trollius chinensis Bge.Be heat and toxic materials clearing away medicine, now made dosage forms such as tablet, granule, capsule, oral liquid in clinical practice.Be mainly used in infectious disease such as upper respiratory tract infection, pharyngitis, tonsillitis.Effective ingredient is a total flavones, is mainly apigenin-8-C-glucoside and orientin.The extraction process of the existing various dosage forms of Flos Trollii is all continued to use the tablet extraction process, and Flos Trollii is carried secondary with decocting in water, is condensed into the preparation that extractum is made, and general flavone content all is no more than 15%.
The present medicinal flower part of only using of Flos Trollii, and the ground stem and leaf part behind the harvesting flower is not effectively utilized, through systematic study to its chemical constituent and bacteriostatic activity, flavone component is consistent in showing stem and leaf part total flavones and spending, fungistatic effect with spend quite, just flavones content only is 1/8 of flower.If continue to use the tablet extraction process: the Flos Trollii stem and leaf is carried secondary with decocting in water, be condensed into extractum and make preparation, extract total flavones and become the separating/enriching difficulty, do not reach the required purity of preparation, can't use aborning.
Goal of the invention: for improving the extract concentrations of Flos Trollii flower, make the contained total flavones of stem and leaf part can effectively obtain utilizing simultaneously, the invention provides a kind of Flos Trollii and Flos Trollii stem leaf total flavones extraction process, can improve extract purity, flavones content is reached more than 50%, so that prepare various high purity products.
Technical scheme: with Flos Trollii stem and leaf or Flos Trollii is raw material, micronizing, and extract at low temperature, resin absorption makes and contains the dry extract of total flavones of tropaeolum more than 50%.
The extraction process flow process is: (1) Flos Trollii stem and leaf (or Flos Trollii) is dried to water content below 8%.(2) pulverizing with micronizing equipment is the above micropowders of 300 orders.(3) water of adding PH7-8,50 ℃ of stirring and leaching.(4) residue is removed in centrifugal filtration.(5) medicinal liquid passes through macroporous resin adsorption.(6) with 70% ethanol elution resin.(7) reclaim ethanol.(8) spray drying obtains total flavones of tropaeolum dry extract content more than 50%.
The concrete operations step is as follows:
(1) gets exsiccant Flos Trollii stem and leaf (or Flos Trollii), require water content below 8%, be crushed to 300 order micropowders with super micron mill, add the pure water of PH7-8, amount of water is 14 times of medicated powder, under 50 ℃ of temperature, stirring and leaching, 30 minutes time, centrifugal filtration, medicinal liquid is stored, and medicinal residues continue to add 10 times of the pure water of PH7-8, under 50 ℃ of temperature, stirring and leaching 20 minutes, centrifugal filtration, medicinal residues discard, twice medicinal liquid merges, and is filtered to clarification, and be standby.
(2) above medicinal liquid is by AB-8 type macroporous adsorbent resin, and flow velocity is 2 times of amount of resin per hour, treats medicinal liquid all by behind the resin, reuse 70% ethanol elution, flow velocity for per hour 2.5 times measure resins, the ethanol consumption is 10 times of amount of resin.
(3) ethanol elution reclaims ethanol to there not being the alcohol flavor, and the fluid extract spray drying gets dry extract, measures content.
Beneficial effect: owing to adopt superfine powder to extract, make the plant cell fragmentation after, leach at low temperature has following distinguishing feature than traditional method:
(1) the extraction temperature is low.Adopt present technique to extract temperature and be no more than 50 ℃, effective component extraction rate surpasses tradition and boils the mode of putting forward.Avoid traditional decoction to extract the destruction that heating process causes effective ingredient, not only effectively kept effective ingredient, greatly saved the energy simultaneously.
(2) extraction time is short.A normal extraction time has only 30 minutes, more than 1 times, has improved work efficiency than traditional extraction time shortening.
(3) effective component extraction rate height.Intracellular effective ingredient can all be discharged in the solvent after cell wall breaking, has not only improved the yield of flower effective ingredient, and previously discarded Flos Trollii stem and leaf part effectively can also be used, and has improved resource utilization greatly.Through measuring, this technology total flavones of tropaeolum extraction ratio improves 1.2 times than traditional decoction extraction ratio.
(4) product purity improves greatly.Macroporous resin adsorption makes at utmost enrichment of total flavones of tropaeolum.Impurity removal is complete, and product purity is improved greatly.
Embodiment 1:(1) gets dry Flos Trollii stem and leaf 1kg,, be crushed to more than 300 orders with the doubly sharp pulverizing mill of 6 types, add the pure water 14kg of PH7-8, be heated to 50 ℃, stir, lixiviate 30 minutes is filtered with centrifugal filter, and medicinal liquid is stored, medicinal residues continue to add pure water 10kg, equally under 50 ℃ of temperature, and stirring and leaching 20 minutes, centrifugal filtration, medicinal residues discard, and merge lixiviating solution 20kg twice, precipitate 24 hours, be filtered to clarification.
(2) above medicinal liquid with 2 times of resins/hour flow velocity by macroporous resin column, treat that medicinal liquid all passes through after.With the purified rinse water resin column once, reuse 70% ethanol elution, eluting ethanol flow velocity be 2.5 times of resins/hour, the ethanol consumption is 10 times of amount of resin, it is colourless to be washed till effluent.
(3) ethanol elution reclaims ethanol to there not being the ethanol flavor, and spray drying gets dry extract 55g, and measuring total flavones of tropaeolum content is 51.4%.
Embodiment 2: with Flos Trollii is raw material, and concrete operations are identical with example 1.Get total flavones powder 216g, general flavone content is 54%.
Claims (1)
1, a kind of extracting method of total flavones of tropaeolum is characterized in that its method step is as follows: (1) Flos Trollii stem and leaf (or Flos Trollii) is dried to water content below 8%; (2) pulverizing with micronizing equipment is the above micropowders of 300 orders; (3) water of adding PH7-8,50 ℃ of stirring and leaching; (4) residue is removed in centrifugal filtration; (5) medicinal liquid passes through macroporous resin adsorption; (6) with 70% ethanol elution resin; (7) reclaim ethanol; (8) spray drying obtains the total flavones of tropaeolum dry extract, and content is more than 50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200810166951A CN101683396A (en) | 2008-09-28 | 2008-09-28 | Extraction method of tropaeolum total lavonoids |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810166951A CN101683396A (en) | 2008-09-28 | 2008-09-28 | Extraction method of tropaeolum total lavonoids |
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| CN101683396A true CN101683396A (en) | 2010-03-31 |
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| CN200810166951A Pending CN101683396A (en) | 2008-09-28 | 2008-09-28 | Extraction method of tropaeolum total lavonoids |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670634A (en) * | 2011-03-09 | 2012-09-19 | 苏州世林医药技术发展有限公司 | C-glycosylflavones composition, preparation method and application thereof |
| CN104324094A (en) * | 2014-11-25 | 2015-02-04 | 中国人民解放军第三军医大学第一附属医院 | Application of globeflower flavonoids in preparation of medicine for treating itch dermatoses |
| CN106198166A (en) * | 2016-07-12 | 2016-12-07 | 广西壮族自治区中医药研究院 | The extraction of a kind of blackwort symphytum total flavones and assay method |
| CN109602792A (en) * | 2018-12-11 | 2019-04-12 | 江西赣隆药业有限公司 | A kind of trollflower broken wall medicine materical crude slice and preparation method thereof |
-
2008
- 2008-09-28 CN CN200810166951A patent/CN101683396A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670634A (en) * | 2011-03-09 | 2012-09-19 | 苏州世林医药技术发展有限公司 | C-glycosylflavones composition, preparation method and application thereof |
| CN104324094A (en) * | 2014-11-25 | 2015-02-04 | 中国人民解放军第三军医大学第一附属医院 | Application of globeflower flavonoids in preparation of medicine for treating itch dermatoses |
| CN106198166A (en) * | 2016-07-12 | 2016-12-07 | 广西壮族自治区中医药研究院 | The extraction of a kind of blackwort symphytum total flavones and assay method |
| CN106198166B (en) * | 2016-07-12 | 2019-03-29 | 广西壮族自治区中医药研究院 | A kind of extraction and measuring method of black grass general flavone |
| CN109602792A (en) * | 2018-12-11 | 2019-04-12 | 江西赣隆药业有限公司 | A kind of trollflower broken wall medicine materical crude slice and preparation method thereof |
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Open date: 20100331 |