CN107050188A - Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared - Google Patents
Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared Download PDFInfo
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- CN107050188A CN107050188A CN201710322150.8A CN201710322150A CN107050188A CN 107050188 A CN107050188 A CN 107050188A CN 201710322150 A CN201710322150 A CN 201710322150A CN 107050188 A CN107050188 A CN 107050188A
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- CN
- China
- Prior art keywords
- matrimony vine
- decocting liquid
- heroin
- retinal tissue
- purposes
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared, the matrimony vine decocting liquid is the crude drug decocting liquid of 0.2 ~ 0.4g/mL Han crude drug amount.The present invention is on the basis of numerous studies are carried out to matrimony vine; it was found that matrimony vine decocting liquid can improve histiocytic SOD vigor; suppress cytolipin peroxidating and inflammatory reaction; suppress cell iNOS activity; weaken institutional framework damage, reduce Bax, Caspase 3, the expression of TNF α, NF kB proteins, increase cell viability; with the effect such as anti-oxidant, anti-inflammatory and protection retina, it effectively can slow down or mitigate damage of the heroin to retinal tissue.The new purposes of matrimony vine decocting liquid is added, there is certain reference significance to the following matrimony vine medicine resource with west region character that develops.
Description
Technical field
Treatment heroin retina is being prepared the present invention relates to a kind of Chinese medicine decocting liquid, more particularly to a kind of matrimony vine decocting liquid
Purposes in tissue damage medicine.
Background technology
Heroin is diacetylmorphine, be it is a kind of seriously endanger life opioid, easily through blood-brain barrier with
Opiate receptor in central nervous system and surrounding tissue is combined, with stronger analgesic activity, and Central nervous has strongly
Agonism, if be used for a long time or improper use, serious pharmacological dependence can be produced, central nervous system of being overexcited,
The normal expression of the balance of interference body antioxidant system and related differential protein, has pathologic damage to body tissue structure.
Therefore, protective effect of the research Chinese herbal medicine to animal or human body, the Chinese medicine that research and development are had no toxic side effect to body and antiradiation drug is damaged
Preparation, the development for medical career is significant.
At present, the excitant such as heroin abuse, abuse that phenomenon is more universal, Adverse Event occurs repeatedly, and has a strong impact on
Human health and social stability.Now generally eliminate excitant habituation using methadone, clonidine etc. are medicinal both at home and abroad or give up
Symptom, but these medicines have certain additive and drug resistance problems in itself, therefore selection toxic side effect is small, dependence is small
And the small Chinese medicine of drug resistance, there is important meaning for the damage reduced or reduction upper is caused to body tissue organ
Justice.There is presently no the middle medicine that the anti-heroin retinal tissue of special efficacy is damaged.
The content of the invention
The present invention provides a kind of matrimony vine decocting liquid of anti-heroin retinal tissue damage, with reach anti-heroin damage,
The purpose of protective tissue cell, promotion organization cell repair.
Therefore, the technical scheme used for：
Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared.
Containing the matrimony vine decocting liquid that crude drug amount is 0.2 ~ 0.4g/mL in treatment heroin retinal tissue damage medicine is prepared
Purposes.
The process for producing of the present invention is simple, it is easy to large-scale production；Matrimony vine dietotherapeutic, pollution-free, small investment, medicine
Price is honest and clean, convenient material drawing；Toxicological experiment finds no toxicity；There is significant protection to the damage of heroin retinal tissue
Effect.
Matrimony vine decocting liquid can improve histiocytic SOD vigor, suppress cytolipin peroxidating and inflammatory reaction, suppress thin
Born of the same parents iNOS activity, weakens institutional framework damage, reduces Bax, Caspase-3, TNF-α, the expression of NF- kB proteins, increase cell
Vigor, with the effect such as anti-oxidant, anti-inflammatory and protection retina, effectively can slow down or mitigate damage of the heroin to retinal tissue
Wound.
Brief description of the drawings
Fig. 1 is the change of Mouse Retina institutional framework of the present invention；
GCL. ganglion-cell layer, INL. inner nuclear layers, ONL. outer nuclear layers；RCL. the cone retinal rod layer；↑ gangliocyte vacuolations； *.
The loose disorder of inner nuclear layer；The loose disorder of ※ outer nuclear layers；HE is dyed, scale=25 μm（Similarly hereinafter）
A. blank control group 15d Mouse Retinas institutional framework, retina structure is complete, and each confluent monolayer cells are high-visible；
B. experimental comparison group 15d Mouse Retinas institutional framework, GCL layers of gangliocyte reduce and the denaturation of vacuole sample occur,
INL and ONL structure disturbances, loose reticulate；
C-D. matrimony vine 1,2 groups of 15d Mouse Retina institutional frameworks, retinal tissue damage is obviously improved, GCL layer sky of retina
Alveolation mitigates, and INL and ONL layers of arrangement are more neat, close.
Fig. 2 is the change of Mouse Retina tissue Bax protein expressions of the present invention；
A. the expression of blank control group 15d Mouse Retinas tissue Bax albumen；
B. the expression of experimental comparison group 15d Mouse Retinas tissue Bax albumen；
C-D. matrimony vine 1, the expression of 2 groups of 15d Mouse Retina tissue Bax albumen
Fig. 3 is the change of Mouse Retina tissue Caspase-3 protein expressions of the present invention
A. the expression of blank control group 15d Mouse Retinas tissue Caspase-3 albumen；
B. the expression of experimental comparison group 15d Mouse Retinas tissue Caspase-3 albumen；
C-D. matrimony vine 1, the expression of 2 groups of 15d Mouse Retina tissue Caspase-3 albumen
Fig. 4 is the change of Mouse Retina tissue T NF- α protein expressions of the present invention
A. the expression of blank control group 15d Mouse Retinas tissue T NF- α albumen；
B. the expression of experimental comparison group 15d Mouse Retinas tissue T NF- α albumen；
C-D. matrimony vine 1, the expression of 2 groups of 15d Mouse Retina tissue T NF- α albumen
Fig. 5 is the change that Mouse Retina tissue NF- kB proteins of the present invention are expressed；
A. the expression of blank control group 15d Mouse Retinas tissue NF- kB proteins；
B. the expression of experimental comparison group 15d Mouse Retinas tissue NF- kB proteins；
C-D. matrimony vine 1, the expression of 2 groups of 15d Mouse Retina tissue NF- kB proteins
Embodiment
The present invention is further illustrated with reference to embodiment.
The preparation method of decoction of the present invention
According to traditional Chinese medicine decoction preparation method, lycium barbarum 100g is taken, 30min is boiled after the 1L that adds water immersions 1h, filters concoction,
The dregs of a decoction add water decoction 30min filtration concoctions, merge decoction twice, then decoction concentration is made 0.2 with Rotary Evaporators ~
0.4g/mL crude drug decocting liquid is used for tests below.
Protective effect of the matrimony vine decocting liquid to heroin retinal damage
Raw 10d kunming mice 120 is selected, blank control group, experiment contrast is randomly divided into（Heroin）Group and matrimony vine decocting
Liquid（Heroin+matrimony vine）1st, 2 groups.Experimental comparison group and matrimony vine decocting liquid 1,2 groups use ascending-dose（2.0g/L、3.0g/L、
4.0g/L）Heroin solution is continuous（1 ~ 5d, 6 ~ 10d, 11 ~ 15d）15d, per injection 0.2mL (twice daily) is injected intraperitoneally,
Inject after heroin 1h, matrimony vine decocting liquid 1,2 groups distinguish gavage 0.2g/mL, 0.4g/mL again matrimony vine Aqueous extracts, experiment contrast
The physiological saline of the physiological saline of group gavage equivalent, blank control group intraperitoneal injection and gavage equivalent.In experiment 5d, 10d and 15d
When, each group mouse is put to death, with colorimetric determination Mouse Retina tissue superoxide dismutase（SOD）, inducible nitric oxide
Synthase（iNOS）Activity and MDA（MDA）Content and blood plasma Plasma Glutathione（GSH）Content and lactic dehydrogenase（LDH）It is living
Property, the pathological change of Mouse Retina institutional framework is observed with paraffin imbedding and HE decoration methods, is examined with immunohistochemical method
Mouse Retina tissue Bax, Caspase-3, TNF-α, the change of NF- kB protein expression quantity are surveyed, with Image-proplus 6.0
Image analysis software detection Bax, Caspase-3, TNF-α, NF- kB proteins are in the positives reaction product of Mouse Retina tissue
Average optical density, experimental data carries out statistical analysis with SPSS 13.0, as a result with mean+SD (Mean ± SD) table
Show, research matrimony vine decocting liquid causes the influence of retinal tissue damage to heroin.Experiment finds experimental comparison group Mouse Retina
Tissue SOD activity pole significantly reduce (P<0.01), iNOS activity and MDA contents extremely significantly rise (P<0.01)；Blood plasma GSH contents
Pole significantly reduce (P<0.01), LDH activity significantly raise (P<0.01 orP<0.05)；Retinal tissue pathologic damage is obvious,
Ganglion-cell layer（GCL）Simultaneously there is the denaturation of vacuole sample, inner nuclear layer in Leukopenia（INL）And outer nuclear layer（ONL）Structure disturbance, it is loose into
It is netted, dye uneven；Retinal tissue Bax, Caspase-3, TNF-α, the average optical density value of NF- kB protein positive expressions
Significantly increase, dyeing intensification (P<0.01 orP<0.05).Matrimony vine（Heroin+matrimony vine）1st, 2 groups of Mouse Retina tissue SOD activity
Again raise, iNOS activity and MDA contents reduction, when 10d, 15d significant difference or extremely significantly (P<0.01 orP<0.05)；Retina
Tissue damage has clear improvement, and GCL layers of vacuolation of retina mitigate, and INL and ONL layers of arrangement are more neat, close, dye more equal
It is even；Blood plasma GSH contents are raised again, LDH activity reduction, significant difference when 10d, 15d (P<0.05 orP<0.01)；Retinal tissue
Bax, Caspase-3, TNF-α, the average optical density value of NF- kB protein positive expressions are obviously reduced, dyeing decrease (P<0.01 orP<0.05).As a result Fig. 1 ~ 5, table 1 ~ 3 are seen.
In summary, matrimony vine decocting liquid energy effectively improves histiocytic antioxidation activity, reduces free radical to retina
Histiocytic damage, increases cell membrane stability, promotes retinal tissue cell metabolism, suppresses or neutralize the work of inflammatory factor
Property, it can substantially reduce heroin and cause damage mice plasma LDH activity and retinal tissue MDA（MDA）Content（Table 1,2）, it is bright
It is aobvious to mitigate retinal tissue cell degeneration（Fig. 1）, suppress or reduce Bax, Caspase-3, TNF-α, NF- kB proteins in view
Expression in membrane tissue cell（Fig. 2,3,4,5, table 3）, increase cell viability, thus tissue damage caused by mitigating heroin and
Apoptosis.Meanwhile, matrimony vine decocting liquid also has safety, without dependence, nontoxic effect.
Claims (2)
1. purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared.
2. purposes according to claim 1, it is characterised in that：Exist containing crude drug amount for 0.2 ~ 0.4g/mL matrimony vine decocting liquid
Prepare the purposes in treatment heroin retinal tissue damage medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710322150.8A CN107050188A (en) | 2017-05-09 | 2017-05-09 | Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710322150.8A CN107050188A (en) | 2017-05-09 | 2017-05-09 | Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared |
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| CN107050188A true CN107050188A (en) | 2017-08-18 |
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| CN201710322150.8A Pending CN107050188A (en) | 2017-05-09 | 2017-05-09 | Purposes of the matrimony vine decocting liquid in treatment heroin retinal tissue damage medicine is prepared |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112843150A (en) * | 2020-01-08 | 2021-05-28 | 中国科学院生物物理研究所 | Composition containing wolfberry extract and preparation method and application method thereof |
Citations (3)
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|---|---|---|---|---|
| CN101474311A (en) * | 2009-01-22 | 2009-07-08 | 哈尔滨金道堂生物科技有限公司 | Soft capsule for alleviating eyestrain and preparation method thereof |
| CN104524099A (en) * | 2014-12-07 | 2015-04-22 | 四川金堂海纳生物医药技术研究所 | Decoction medicine for treating central retinitis, and preparation method thereof |
| US20160151437A1 (en) * | 2014-06-02 | 2016-06-02 | Viva Pharmaceutical, Inc. | Nutritional supplements for improved vision and methods of treating the same |
-
2017
- 2017-05-09 CN CN201710322150.8A patent/CN107050188A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474311A (en) * | 2009-01-22 | 2009-07-08 | 哈尔滨金道堂生物科技有限公司 | Soft capsule for alleviating eyestrain and preparation method thereof |
| US20160151437A1 (en) * | 2014-06-02 | 2016-06-02 | Viva Pharmaceutical, Inc. | Nutritional supplements for improved vision and methods of treating the same |
| CN104524099A (en) * | 2014-12-07 | 2015-04-22 | 四川金堂海纳生物医药技术研究所 | Decoction medicine for treating central retinitis, and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 苏国辉: "枸杞子与视网膜保护", 《中国眼镜科技杂志》 * |
| 魏波: "海洛因对视力影响的观察", 《华中医学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112843150A (en) * | 2020-01-08 | 2021-05-28 | 中国科学院生物物理研究所 | Composition containing wolfberry extract and preparation method and application method thereof |
| CN112843150B (en) * | 2020-01-08 | 2022-03-15 | 中国科学院生物物理研究所 | Composition containing wolfberry extract and preparation method and application method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170818 |