CN110354163A - Purposes of the licorice water extract in treatment heroin Renal tissues damage - Google Patents
Purposes of the licorice water extract in treatment heroin Renal tissues damage Download PDFInfo
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- CN110354163A CN110354163A CN201910605052.4A CN201910605052A CN110354163A CN 110354163 A CN110354163 A CN 110354163A CN 201910605052 A CN201910605052 A CN 201910605052A CN 110354163 A CN110354163 A CN 110354163A
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- heroin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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Abstract
Purposes of the licorice water extract in treatment heroin Renal tissues damage, is the crude drug decocting liquid of 0.2 ~ 0.4g/mL.Present invention discover that licorice water extract can reduce plasma creatinine, uric acid content, cell Na can be improved+‑K+ATP、Ca2+‑Mg2+Atpase activity; reduce nephridial tissue lactic dehydrogenase (LDH) activity and MDA content; cell membrane can be protected, maintain intraor extracellular ionic equilibrium; the expression of nephridial tissue aquaporin (AQP2) can be enhanced; nephridial tissue activity of catalase can be improved; nephridial tissue cellular damage caused by heroin can be effectively reduced, mitigates and repairs and damaged caused by heroin, have good protective effect to Renal tissues damage caused by heroin.
Description
Technical field
The present invention relates to a kind of Chinese traditional medicine water extract more particularly to a kind of licorice water extracts of anti-heroin Renal tissues damage.
Background technique
Heroin, that is, diacetylmorphine is to abuse most extensive, the strongest drug of dependence in opioid drug, easily penetrates
Blood-brain barrier has stronger analgesic activity in conjunction with the opiate receptor in central nervous system and surrounding tissue, and to maincenter
Nerve has strong agonism, if long-time service or improper use, can generate psychology and physiological dependence and serious drug
It relies on, central nervous system of being overexcited, the normal expression of the balance of interference body antioxidant system and related differential protein is right
Body tissue structure has pathologic damage, seriously affects the structure of the tracts such as body nerve, breathing, immune, circulation, and meeting
Smoker is caused to generate the psychological problems such as lather, dejected, decadent, depressed, vexed, self-closing.Therefore, research Chinese herbal medicine is to dynamic
The protective effect of object or human body, research and development have no toxic side effect to body and the Chinese materia medica preparation of antiradiation drug damage, for medical career
Development is of great significance.
Excitant habituation or withrawal symptom, but this are generally eliminated outside Current Domestic using medicines such as methadone, clonidines
There is certain additive and drug resistance problems for a little drugs itself.Researches show that there is presently no the anti-extra large Lip river kidneys of special efficacy
The middle drug of tissue damage, therefore the Chinese medicine that toxic side effect is small, dependence is small and drug resistance is small is chosen, it is emerging for mitigating or reducing
Putting forth energy property drug is damaged caused by body tissue organ and is of great significance.
Summary of the invention
The present invention provides a kind of licorice water extract of anti-heroin Renal tissues damage, to reach anti-heroin damage, protection
Histocyte promotes the purpose of histocyte reparation.
The purposes of licorice water extract of the invention in anti-heroin Renal tissues damage is exactly licorice water extract as treatment
Application in heroin Renal tissues damage, the concentration of the licorice water extract are containing 0.2 ~ 0.4g/mL of crude drug amount.
The research of the invention finds that licorice water extract can be such that weight, kidney weight and renal index increases, plasma creatinine, urine can be reduced
Acid content can improve cell Na+-K+ATP、Ca2+-Mg2+Atpase activity, can reduce nephridial tissue lactic dehydrogenase (LDH) activity and
MDA content can protect cell membrane, maintain intraor extracellular ionic equilibrium, nephridial tissue activity of catalase can be improved, can enhance kidney
The expression for organizing aquaporin (AQP2) can effectively reduce nephridial tissue cellular damage caused by heroin, mitigate and repair sea
Lip river is damaged because caused by, has good protective effect to Renal tissues damage caused by heroin.
Process for producing of the present invention is simple, pollution-free, and small investment, drug is inexpensive, and materials are convenient.With curative effect height, nothing
Toxic side effect, it is convenient to take the features such as, be suitable for that sanatorium, hospital and family use.
Detailed description of the invention
Fig. 1 is influence of the licorice water extract to heroin Renal tissues damage and AQP2 protein expression；In figure:
A. 10 d cortex renis of blank control group, HE dyeing；
B. heroin group 15d cortex renis, the serious pyknosis of capillary ball, renal tubule swelling, necrosis, nucleus degradation；
C. 10 d cortex renis of Radix Glycyrrhizae group, renal proximal tubules and distal convoluted tubule are more complete；
D. Radix Glycyrrhizae group 15d cortex renis, kidney proximal tubule have slight swelling；
E. blank control group 15d cortex renis, AQP2 albumen are expressed in proximal tubule and distal tubule in strong positive；
F. heroin group 10d cortex renis, AQP2 albumen have expression in kidney proximal tubule and distal convoluted tubule；
G. Radix Glycyrrhizae group 15d cortex renis, AQP2 albumen are expressed in proximal tubule and distal tubule in strong positive；
H. Radix Glycyrrhizae group 15d kidney medulla, AQP2 are expressed in concetrated pipe in strong positive；
Dt. distal convoluted tubule；Pt. proximal convoluted tubule；Rc. glomerulus；Scale=30 μm.
Specific embodiment
The present invention is further illustrated below with reference to embodiment.
Medical fluid preparation
Licorice water extract boils 30 min the preparation method comprises the following steps: 100 g of extracting liquorice crude drug after adding water 1L to impregnate 1 h, filter medicine
Juice, the dregs of a decoction, which add water, decocts 30 min filtration decoction, merges medical fluid twice, then decoction concentration is made with Rotary Evaporators
The crude drug decocting liquid of 0.2 ~ 0.4g/mL.
Protective effect of the licorice water extract to heroin Renal tissues damage
Select raw 8 d age kunming mices 90 (Lanzhou University's medical faunae center), 7 d of adaptive feeding before testing, then with
Machine is divided into blank control group, experiment contrast (heroin) group and Radix Glycyrrhizae (heroin+Radix Glycyrrhizae) group, and experimental comparison group and Radix Glycyrrhizae group exist
15 d of heroin solution, per injection 0.2mL is injected intraperitoneally with ascending-dose (2,3,4 g/L) in l-5,6-10,11-15 d, often
It twice (9:20 am, 16:20 pm), inject heroin half an hour after 0.2 ml/10 g weight of Radix Glycyrrhizae group stomach-filling (1 ml containing gives birth to
The licorice water extract of medicine 0.2 g), the physiological saline of heroin group stomach-filling equivalent, blank control group intraperitoneal injection and stomach-filling equivalent
Physiological saline；When testing 5 d, 10 d and 15 d, each group mouse is put to death, is weighed with electronic balance weighing mouse weight and kidney
Variation, calculates the variation of renal index, with colorimetric method and V-5000 visible spectrophotometer detection Plasma Uric Acid (UA) and creatinine
(Cr) with nephridial tissue malonaldehyde (MDA) content and catalase (CAT), Na+-K+ATP enzyme, Ca2+-Mg2+ATP enzyme, lactic acid are de-
Hydrogen enzyme (LDH) active variation, with the pathological change of paraffin imbedding and HE decoration method observation kidney of mouse structure, with immune
Histochemical method detects the variation of nephridial tissue aquaporin (AQP2) expression quantity, with 6.0 image analysis of Image-proplus
The average optical density of software detection aquaporin (AQP2) positive reaction product in nephridial tissue, experimental data SPSS
13.0 is for statistical analysis, is as a result indicated with mean+SD (Mean ± SD), and research licorice water extract causes heroin
The influence of Renal tissues damage.Research find experiment contrast (heroin) group mouse weight, kidney weight reduce (P< 0.05,P<
0.01), blood plasma UA and Cr content it is significantly raised (P< 0.05,P< 0.01), nephridial tissue MDA content increases, nephridial tissue peroxidating
Hydrogen enzyme, Na+-K+ATP enzyme, Ca2+-Mg2+The extremely significant reduction of atpase activity (P< 0.01), lactic dehydrogenase (LDH) activity is significant rises
High (P< 0.05,P< 0.01), renal pathology damage is obvious, the pyknosis of Malpighian corpuscle capillary ball, Malpighian corpuscle soft edge,
Nuclear targeting is deepened, and occurs degrading and apparent apoptosis, tubular ectasia, epithelial cell denaturation, even vacuolation, degradation,
The average optical density value of AQP2 protein positive expression significantly reduces, dyeing shoal (P< 0.05,P< 0.01).And Radix Glycyrrhizae (Hai Luo
Cause+Radix Glycyrrhizae) group mouse weight, kidney again again increase (P< 0.05,P< 0.01), blood plasma UA and Cr content again reduce (P<
0.05,P< 0.01), nephridial tissue catalase, Na+-K+ATP enzyme, Ca2+-Mg2+Atpase activity again it is extremely significant increase (P<
0.05,P< 0.01), nephridial tissue lactic dehydrogenase (LDH) activity and MDA content reduce again (P< 0.05,P< 0.01), Radix Glycyrrhizae
Group mouse cortex renis and medullary substance damage are all also reduced to varying degrees and repair, and renal tubule and concetrated pipe lumen are more regular, cell
Film is complete, and nucleus is high-visible, and the average optical density value of AQP2 protein positive expression obviously increases, dyeing intensification (P<0.01
OrP<0.05).The result is shown in Figure 1, table 1 ~ 5.
The variation of 1 mouse weight of table, kidney weight and renal index
The variation of table 2 Plasma Uric Acid, creatinine content
3 nephridial tissue catalase of table, lactic dehydrogenase, Na+ -K+ATP enzyme, Ca2+ -Mg2+Atpase activity and malonaldehyde contain
The variation of amount
*P< 0.05, * *P< 0.01 is compared with blank control group；# P< 0.05,## p< 0.01 is compared with experimental comparison group.
The variation of 4 nephridial tissue AQP2 protein expression of table
The variation of 5 glomerulus diameter of table
In conclusion licorice water extract can make mouse weight, kidney weight and renal index increase (table 1), plasma creatinine, uric acid can be reduced
Content (table 2), can improve cell Na+-K+ATP、Ca2+-Mg2+Atpase activity reduces nephridial tissue lactic dehydrogenase (LDH) activity
With MDA content, cell membrane can be protected, maintain intraor extracellular ionic equilibrium, nephridial tissue activity of catalase (table 3) can be improved,
The expression (table 4) that nephridial tissue AQP2 albumen can be enhanced, can effectively reduce nephridial tissue cellular damage caused by heroin, mitigate and repair
Damage (Fig. 1, table 5) caused by multiple heroin, has good protective effect to Renal tissues damage caused by heroin.And use peace
Entirely, no dependence, no obvious toxic-side effects.
Claims (3)
1. purposes of the licorice water extract in treatment heroin Renal tissues damage.
2. purposes according to claim 1, it is characterised in that: exist containing the licorice water extract that crude drug amount is 0.2 ~ 0.4g/mL
Purposes in anti-heroin Renal tissues damage.
3. purposes according to claim 1 or 2, it is characterised in that: licorice water extract can reduce plasma creatinine, uric acid contains
Amount, can improve cell Na+-K+ATP、Ca2+-Mg2+Atpase activity reduces nephridial tissue lactate dehydrogenase L DH activity and MDA content,
Cell membrane can be protected, maintain intraor extracellular ionic equilibrium, the expression of nephridial tissue aquaporin AQP2 can be enhanced, kidney group can be improved
Knit activity of catalase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910605052.4A CN110354163A (en) | 2019-07-05 | 2019-07-05 | Purposes of the licorice water extract in treatment heroin Renal tissues damage |
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| CN201910605052.4A CN110354163A (en) | 2019-07-05 | 2019-07-05 | Purposes of the licorice water extract in treatment heroin Renal tissues damage |
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| CN201910605052.4A Pending CN110354163A (en) | 2019-07-05 | 2019-07-05 | Purposes of the licorice water extract in treatment heroin Renal tissues damage |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131033A (en) * | 1995-09-08 | 1996-09-18 | 肖利平 | Traditional Chinese medicine oral liquor for narcotics abstinence |
| US20070289258A1 (en) * | 2006-06-14 | 2007-12-20 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Individualized pharmaceutical selection and packaging |
-
2019
- 2019-07-05 CN CN201910605052.4A patent/CN110354163A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131033A (en) * | 1995-09-08 | 1996-09-18 | 肖利平 | Traditional Chinese medicine oral liquor for narcotics abstinence |
| US20070289258A1 (en) * | 2006-06-14 | 2007-12-20 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Individualized pharmaceutical selection and packaging |
Non-Patent Citations (2)
| Title |
|---|
| 何玲慧等: "海洛因对小鼠肾结构与功能的影响", 《解剖学报》 * |
| 毕礼明等: "甘草治疗肾病研究进展", 《中国实验方剂学杂志》 * |
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Application publication date: 20191022 |