CN104689251B - A kind of Chinese medicine composition, its preparation method and Chinese medicine preparation for alleviating chemical damage - Google Patents
A kind of Chinese medicine composition, its preparation method and Chinese medicine preparation for alleviating chemical damage Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine composition for alleviating chemical damage, is made up of the component of following parts by weight：The parts by weight of the root of kudzu vine 2~6, the parts by weight of ganoderma lucidum 2~6, the parts by weight of centella 1~5, the parts by weight of rhodiola root 1~5, the parts by weight of radix glycyrrhizae 1~3, the parts by weight of Turmeric P.E 0.1~1.Wherein the root of kudzu vine is clearing heat and detoxicating, and ganoderma lucidum enhancing is immune, and centella is promoting blood circulation and removing blood stasis, and rhodiola root replenishes qi to invigorate the spleen, and is equipped with turmeric promoting the circulation of qi broken stasis of blood, radix glycyrrhizae coordinating the drug actions of a prescription, plays the effect of clearing away damp-heat, relieving alcoholism, soothing the liver courage, row stagnate jointly.Therefore, after above-mentioned several material compoundings, interaction, there is preferable mitigation to chemical damage, so as to play liver-protective effect.Present invention also offers the preparation method of the composition, made of the composition and auxiliary material, Chinese medicine preparation and the composition are preparing the application in having the health products of protective effect to the liver of chemical lesion.
Description
Technical field
The present invention relates to field of health care products, more particularly to a kind of Chinese medicine composition for alleviating chemical damage, its preparation
Method and Chinese medicine preparation.
Background technology
Hepar damnification is the pathology of livers as caused by the factors such as virus, medicine, toxic agent, alcohol, anoxic.Hepatic injury
Mechanism is complex, can be divided into chemically with two kinds of immunity.Chemical mechanism is mainly because the infringement of plasma membrane, free radical damage
The factors such as evil, mitochondrial function imbalance, metabolic disorder cause liver dysfunction.Chemical damage can be because in alcohol, environment
The effect of chemical toxicant and some drugses and cause.
At present, clinically most of chemical damage is caused by alcohol, and thus caused AML also turns into
The focus of social concerns, including mild AML, alcoholic fatty liver, alcoholic hepatitis, alcoholic fibrosis and wine
Essence hepatic sclerosis.The pathogenesis of AML mainly has the direct hepatotoxicity wind agitation of (1) alcohol and its metabolite acetaldehyde；(2)
Oxidative stress damages with active oxygen radical；(3) cell factor and the immune inflammation mechanism of endotaxin mediate；(4) other, such as office
Portion's histanoxia, er stress, iron load increase, Apoptosis etc..Wherein, it is caused a large amount of in alcohol and its metabolic process
Oxidative stress caused by active oxygen radical is considered as the most important early stage pathology mechanism of AML.Take in human body
Alcohol through intestines and stomach absorbed into serum, there are about 90%~95% and complete metabolic conversion in liver rapidly.In liver, ethanol is first by second
The oxidations such as alcohol dehydrogenase (ADH) form acetaldehyde, then form acetic acid through acetaldehyde dehydrogenase (ALDH) oxidation, finally in peripheral tissues
It is degraded to CO2And water.During alcohol metabolism, along with the NADH (NAD+) of oxidation state to reduction
The NADH transformations of state, cause NAD+/NADH ratios to decline, so that biochemical reaction such as tricarboxylic acid cycle, fat dependent on NAD+
The beta-oxidation of fat acid and oxidative phosphorylation, gluconeogenesis etc. are suppressed, and cause glucose -lipid metabolism disorder, so as to cause the fat of liver to become
Property.At the same time, metabolic process can produce superoxide anion, H2O2Deng free radical, activate lipid within endothelial cells peroxidization,
The 26S Proteasome Structure and Function of protein is destroyed, and cell membrane phospholipid is degraded, and membrane passage and flowing sexually revise.Alcohol pair
The activity of liver and SOD2 (SOD) has no significant effect, but can suppress liver and mitochondria glutathione
Peroxidase (GPX1) activity, causes reduced glutathione (GSH) to exhaust, so as to aggravate the oxidative damage of liver.
With the development and growth in the living standard of society, the people of hepatic injury is more and more, particularly alcoholic liver injury.
It is the Poisoning liver diseases caused by long-term excessive consumption of alcohol.In the U.S., alcoholic cirrhosis is that preceding 7 death rates are higher
One of disease, account for more than the 80% of pathogenesis of cirrhosis rate.In recent years, alcohol consumption colony in China's is increasingly huge, conservative estimation, mesh
Preceding at least 5,000,000 people, thus caused AML (ALD) turn into social concerns focus.
Therefore developing a kind of product that can alleviate chemical damage has high market value.
The content of the invention
Present invention solves the technical problem that it is to provide a kind of Chinese medicine composition for alleviating chemical damage, its preparation side
Method and Chinese medicine preparation, the composition have protective effect to the liver of chemical lesion, can be with alleviating chemical damage.
The invention discloses a kind of Chinese medicine composition for alleviating chemical damage, it is made up of the component of following parts by weight：
Preferably, the Turmeric P.E is commercially available.
Preferably, it is made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 2, the parts by weight of ganoderma lucidum 2, the parts by weight of centella 1, the parts by weight of rhodiola root 1, the parts by weight of radix glycyrrhizae 1, turmeric
The parts by weight of extract 0.1.
Preferably, it is made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 6, the parts by weight of ganoderma lucidum 6, the parts by weight of centella 5, the parts by weight of rhodiola root 5, the parts by weight of radix glycyrrhizae 3, turmeric
The parts by weight of extract 1.
Preferably, it is made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 4, the parts by weight of ganoderma lucidum 4, the parts by weight of centella 3, the parts by weight of rhodiola root 3, the parts by weight of radix glycyrrhizae 2, turmeric
The parts by weight of extract 0.4.
Preferably, the preparation method of the Turmeric P.E is：
Take turmeric to crush, extracted 1~5 time with 50% ethanol of 8~12 times of amounts, 2~5h, 60 DEG C of Extracting temperature, is closed every time
And extract solution, filtration, the mass ratio for being concentrated into turmeric and dry cream is 10：1, obtain Turmeric P.E.
The invention discloses a kind of preparation method for the Chinese medicine composition for alleviating chemical damage, comprise the following steps：
(A) by the parts by weight of the root of kudzu vine 2~6, the parts by weight of ganoderma lucidum 2~6, the parts by weight of centella 1~5, the parts by weight of rhodiola root 1~5
With the mixing of the parts by weight of radix glycyrrhizae 1~3, the water for adding 6~20 times of amounts as Extraction solvent, extract 1~4 time, after filtering, filtered
Liquid；
(B) it is 1.0~1.3g/ml the filtrate decompression to be concentrated into concentrated solution density, after spray drying, obtains material；
(C) material is mixed with the parts by weight of Turmeric P.E 0.1~1, the Chinese medicine for the chemical damage that is eased
Composition.
Preferably, the temperature of the extraction is 90~100 DEG C, and the time of extraction is 0.5~3 hour.
The invention discloses composition made from the preparation method described in above-mentioned technical proposal.
The invention also discloses a kind of Chinese medicine preparation for treating chemical damage, as the Chinese medicine described in above-mentioned technical proposal
Composition and pharmaceutically acceptable auxiliary material are made.
The invention also discloses the Chinese medicine composition described in above-mentioned technical proposal to prepare the guarantor of alleviation chemical damage
Application in strong product.
Compared with prior art, the Chinese medicine composition of alleviation chemical damage of the invention, by the group of following parts by weight
Divide and be made：The parts by weight of the root of kudzu vine 2~6, the parts by weight of ganoderma lucidum 2~6, the parts by weight of centella 1~5, the parts by weight of rhodiola root 1~5, radix glycyrrhizae 1
~3 parts by weight, the parts by weight of Turmeric P.E 0.1~1.Wherein the root of kudzu vine is clearing heat and detoxicating, and ganoderma lucidum enhancing is immune, centella promoting blood circulationization
The stasis of blood, rhodiola root replenish qi to invigorate the spleen, and are equipped with turmeric promoting the circulation of qi broken stasis of blood, radix glycyrrhizae coordinating the drug actions of a prescription, play jointly clearing away damp-heat, relieving alcoholism, soothing the liver courage,
The effect of row is stagnant.Therefore, after above-mentioned several material compoundings, interaction, have preferably to alleviate to chemical damage and make
With so as to play liver-protective effect.
Embodiment
For a further understanding of the present invention, the preferred embodiment of the invention is described with reference to embodiment, still
It should be appreciated that these descriptions are simply further explanation the features and advantages of the present invention, rather than to the claims in the present invention
Limitation.
The embodiment of the invention discloses a kind of Chinese medicine composition for alleviating chemical damage, by the component of following parts by weight
It is made：
In the present invention, the component of the selection such as root of kudzu vine, ganoderma lucidum, centella, rhodiola root, radix glycyrrhizae and Turmeric P.E is
Commercially available prod.Wherein, the Turmeric P.E is commercially available that turmeric cellulose content in the Turmeric P.E is not less than 90%.Institute
State the Turmeric P.E that Turmeric P.E is preferably He'nan Zhongda Biological Engineering Co., Ltd.'s production.The Turmeric P.E also may be used
So that according to preparing in a manner of well known to those skilled in the art, the preparation method of the Turmeric P.E is preferably：
Take turmeric to crush, extracted 1~5 time with 50% ethanol of 8~12 times of amounts, 2~5h, 60 DEG C of Extracting temperature, is closed every time
And extract solution, filtration, the mass ratio for being concentrated into turmeric and dry cream is 10：1, obtain Turmeric P.E.
The preparation method of the Turmeric P.E is more preferably：
Take turmeric to crush, extracted 2 times, each 2.5h with 50% ethanol of 10 times of amounts, 60 DEG C of Extracting temperature, merge extraction
Liquid, filtration, the mass ratio for being concentrated into turmeric and dry cream is 10：1, obtain Turmeric P.E.
According to analysis pharmacologically：The root of kudzu vine is clearing heat and detoxicating, and ganoderma lucidum enhancing is immune, and centella is promoting blood circulation and removing blood stasis, rhodiola root benefit
Gas invigorating the spleen, turmeric promoting the circulation of qi broken stasis of blood is equipped with, radix glycyrrhizae coordinating the drug actions of a prescription, plays the stagnant work(of clearing away damp-heat, relieving alcoholism, soothing the liver courage, row jointly
Effect.Therefore, after above-mentioned several material compoundings, interaction, there is preferable mitigation to chemical damage, so as to play
Liver-protective effect.
In the present compositions, preferably it is made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 3~5；The parts by weight of ganoderma lucidum 3~5；
The parts by weight of centella 2~4；The parts by weight of rhodiola root 2~4；
The parts by weight of radix glycyrrhizae 1.5~2.5；The parts by weight of Turmeric P.E 0.2~0.5.
In some embodiments provided by the invention, the liver-protective composition is made up of the component of following weight：
The parts by weight of the root of kudzu vine 2, the parts by weight of ganoderma lucidum 6, the parts by weight of centella 3, the parts by weight of rhodiola root 4, the parts by weight of radix glycyrrhizae 2, turmeric
The parts by weight of extract 0.5.
In some embodiments provided by the invention, the liver-protective composition is made up of the component of following weight：
The parts by weight of the root of kudzu vine 5, the parts by weight of ganoderma lucidum 3, the parts by weight of centella 4, the parts by weight of rhodiola root 2, the parts by weight of radix glycyrrhizae 2, turmeric
The parts by weight of extract 0.3.
In other embodiment provided by the invention, the liver-protective composition by following weight component system
Into：
The parts by weight of the root of kudzu vine 4, the parts by weight of ganoderma lucidum 4, the parts by weight of centella 3, the parts by weight of rhodiola root 3, the parts by weight of radix glycyrrhizae 2, turmeric
The parts by weight of extract 0.4.
The invention discloses a kind of preparation method for the Chinese medicine composition for alleviating chemical damage, comprise the following steps：
(A) by the parts by weight of the root of kudzu vine 2~6, the parts by weight of ganoderma lucidum 2~6, the parts by weight of centella 1~5, the parts by weight of rhodiola root 1~5
With the mixing of the parts by weight of radix glycyrrhizae 1~3, the water for adding 6~20 times of amounts as Extraction solvent, extract 1~4 time, after filtering, filtered
Liquid；
(B) it is 1.0~1.3g/ml the filtrate decompression to be concentrated into concentrated solution density, after spray drying, obtains material；
(C) material is mixed with the parts by weight of Turmeric P.E 0.1~1, the Chinese medicine for the chemical damage that is eased
Composition.
Preferably, the temperature of the extraction is 90~100 DEG C, the time of the extraction is 0.5~3 hour every time.
Preferably, the temperature being concentrated under reduced pressure is 60~70 DEG C.
Preferably, your reading of the concentration is 1.05~1.2g/ml.
Preferably, the hot-air inlets temperature during spray drying is 100~200 DEG C, outlet temperature is 70~100
℃.After the drying, the moisture of the material is controlled below 5%.
Present invention also offers Chinese medicine composition made from above-mentioned preparation method.
Present invention also offers a kind of Chinese medicine preparation for alleviating chemical damage, pharmaceutically may be used by combinations of the above agent
The auxiliary material of receiving is made.The auxiliary material be preferably microcrystalline cellulose, magnesium stearate, Ac-Di-Sol, methyl-silicone oil,
It is lactose, sucrose, mannitol or sorbierite, cornstarch, wheaten starch, rice starch, farina, gelatin, tragacanth, poly-
Vinylpyrrolidone or calcium phosphate.
Chinese medicine preparation provided by the invention can be granule, capsule, tablet, pill or oral liquid.
Present invention also offers the composition described in above-mentioned technical proposal to prepare the health products of alleviation chemical damage
In application.
Effect experiment part, using Ministry of Public Health's issue《Health food is examined and assessment technique specification》In (2003 editions)
" having auxiliary protection function to chemical damage " (carbon tetrachloride hepatic injury model) is studied.Liver caused by carbon tetrachloride damages
Wound is typical chemical damage.After the present invention have studied the liver processing that the composition causes damage to carbon tetrachloride
Action effect.
Effect experiment part is that the experiment that experimental animal carries out liver damage animal model is to generally acknowledge at present from ICR small white mouses
Animal experiment method.Carbon tetrachloride modeling is used first, then to the mice lavage composition of administration carbon tetrachloride, gives four
Chlorination carbon puts to death animal after 24 hours, takes blood system to determine ALT, AST, and take liver to carry out Histopathology detection from serum.
For a further understanding of the present invention, with reference to embodiment to liver-protective composition provided by the invention, its
Preparation method and Chinese medicine preparation are described in detail, and protection scope of the present invention is not limited by the following examples.
In embodiment, the Turmeric P.E is the Turmeric P.E of He'nan Zhongda Biological Engineering Co., Ltd.'s production.
The preparation of the Chinese medicine composition of embodiment 1
By 20 grams of roots of kudzu vine, 20 kairine sesames, 10 grams of centellas, 10 grams of rhodiola roots and 10 grams of radix glycyrrhizae mixing, 700 grams of water are added,
Extracted 1 time at 90 DEG C, extraction time is 0.6 hour；It is 1.0g/ml that density is concentrated under reduced pressure at 60 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 150 DEG C, and outlet temperature is 70 DEG C.
The material is mixed with 1 gram of Turmeric P.E, the Chinese medicine composition for the chemical damage that is eased.
The preparation of the Chinese medicine composition of embodiment 2
By 60 grams of roots of kudzu vine, 60 kairine sesames, 50 grams of centellas, 50 grams of rhodiola roots and 30 grams of radix glycyrrhizae mixing, 2500 grams of water are added,
Extracted 1 time at 95 DEG C, extraction time is 1.5 hours；It is 1.2g/ml that density is concentrated under reduced pressure at 60 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 160 DEG C, and outlet temperature is 80 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, the Chinese medicine composition for the chemical damage that is eased.
The preparation of the Chinese medicine composition of embodiment 3
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1600 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 4 grams of Turmeric P.Es, the Chinese medicine composition for the chemical damage that is eased.
The preparation of the tablet of embodiment 4
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1700 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
By the material, 4 grams of Turmeric P.Es, right amount of auxiliary materials is well mixed, granulation, tabletting, coating, packing, produces this
Invention Chinese medicine composition tablet.
The preparation of the capsule of embodiment 5
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1700 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
By the material, 4 grams of Turmeric P.Es, right amount of auxiliary materials is well mixed, and granulation, encapsulated, packing, produces the present invention
Chinese medicinal composition capsules agent.
The preparation of the granule of embodiment 6
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1700 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
By the material, 4 grams of Turmeric P.Es, right amount of auxiliary materials is well mixed, pelletize, dry, whole grain, packing, produce this
Invention Chinese medicinal composition granules.
The preparation of the pill of embodiment 7
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1700 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
By the material, 4 grams of Turmeric P.Es, right amount of auxiliary materials is well mixed, pill, polishing, coating, packing, produces this
Invention Chinese medicine composition pill.
The preparation of the oral liquid of embodiment 8
By 40 grams of roots of kudzu vine, 40 kairine sesames, 30 grams of centellas, 30 grams of rhodiola roots and 20 grams of radix glycyrrhizae mixing, 1700 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
By the material, 4 grams of Turmeric P.Es, right amount of auxiliary materials is well mixed, and is configured, is dispensed, and produces Chinese medicine group of the present invention
Compound oral liquid.
Comparative example 1
By 60 grams of roots of kudzu vine, 50 grams of centellas, 50 grams of rhodiola roots and 30 grams of radix glycyrrhizae mixing, 1900 grams of water are added, are carried at 100 DEG C
Take 1 time, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；
Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A1.
Comparative example 2
By 60 kairine sesames, 50 grams of centellas, 50 grams of rhodiola roots and 30 grams of radix glycyrrhizae mixing, 1900 grams of water are added, are carried at 100 DEG C
Take 1 time, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；
Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A2.
Comparative example 3
By 60 grams of roots of kudzu vine, 60 kairine sesames, 50 grams of rhodiola roots and 30 grams of radix glycyrrhizae mixing, 2000 grams of water are added, in 100 DEG C of extractions
1 time, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；Institute
Hot-air inlets temperature when stating spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A3.
Comparative example 4
By 60 grams of roots of kudzu vine, 60 kairine sesames, 50 grams of centellas and 30 grams of radix glycyrrhizae mixing, 2000 grams of water are added, in 100 DEG C of extractions
1 time, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；Institute
Hot-air inlets temperature when stating spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A4.
Comparative example 5
By 60 grams of roots of kudzu vine, 60 kairine sesames, 50 grams of centellas and 50 grams of rhodiola root mixing, 2200 grams of water are added, are carried at 100 DEG C
Take 1 time, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；
Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A5.
Comparative example 6
By 60 grams of roots of kudzu vine, 60 kairine sesames, 50 grams of centellas, 50 grams of rhodiola roots and 30 grams of radix glycyrrhizae mixing, 2500 grams of water are added,
Extracted 1 time at 100 DEG C, extraction time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by being spray-dried,
Obtain material；Hot-air inlets temperature during the spray drying is 200 DEG C, and outlet temperature is 100 DEG C, obtains composition A6.
Comparative example 7
By 60 kairine sesames, 50 grams of centellas and 50 grams of rhodiola root mixing, 1600 grams of water are added, extracts 1 time, carries at 100 DEG C
It is 3 hours to take the time；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；The spraying
Hot-air inlets temperature when drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A7.
Comparative example 8
50 grams of rhodiola roots and 30 grams of radix glycyrrhizaes are mixed, add 800 grams of water, are extracted 1 time at 100 DEG C, extraction time is 3 small
When；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；The heat during spray drying is empty
Gas inlet temperature is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A8.
Comparative example 9
By 60 grams of roots of kudzu vine, 50 grams of centellas and 50 grams of rhodiola root mixing, 1600 grams of water are added, extracts 1 time, carries at 100 DEG C
It is 3 hours to take the time；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；The spraying
Hot-air inlets temperature when drying is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A9.
Comparative example 10
By 60 grams of roots of kudzu vine, 50 grams of centellas and 30 grams of radix glycyrrhizae mixing, 1400 grams of water are added, are extracted 1 time at 100 DEG C, extraction
Time is 3 hours；It is 1.3g/ml that density is concentrated under reduced pressure at 70 DEG C, by spray drying, obtains material；The spraying is dry
Hot-air inlets temperature when dry is 200 DEG C, and outlet temperature is 100 DEG C.
The material is mixed with 10 grams of Turmeric P.Es, obtains composition A10.
The test of pesticide effectiveness of 9 composition provided by the invention of embodiment
ICR small white mouses used in experiment, cleaning grade, 18~22g, male, SPF levels, purchased from Yangzhou University's comparative medicine center,
Quality certification number：SCXK (Soviet Union) 27~0001.
Blank group：Distilled water is given, according to 0.2ml/10g body weight to mouse stomach.
Model group：Distilled water is given, according to 0.2ml/10g body weight to mouse stomach,.
Experimental group：Composition prepared by embodiment 1~3 and comparative example 1~10, gives according to every 1 kg body weight of mouse
The standard that 0.638 gram of composition, composition is configured to the aqueous solution, according to 0.2ml/10g body weight to mouse stomach.
150 ICR mouse are randomly divided into 15 groups, every group 10.First 5 days, daily oral gavage give embodiment 1~3 and
Composition in comparative example 1~10, blank group, model group give distilled water.6th day, model group and each experimental group gave volume
The carbon tetrachloride that fraction is 0.5%/olive oil solution 0.1ml/10g body weight intraperitoneal injection, after intraperitoneal injection, each group animal fasting
17 hours, but still give drinking water.Blank group is to olive oil.After 17 hours, blood system is taken to take blood system from serum, measure from serum
ALT, AST, and take 0.6 × 0.6 × 0.2mm32 pieces of liver organizations carry out Histopathology detection.By fixed liver organization
Sample is dehydrated respectively, waxdip, embedding, 4 μm of slice thick, HE dyeing, light Microscopic observation hepatic pathology change, from intend degeneration of liver cells
Grade scale (is shown in Table 1).
Table 1：Degeneration of liver cells grade scale
ALT (glutamic-pyruvic transaminase) activity uses reitman-frankel method；AST (glutamic-oxalacetic transaminease) activity uses reitman-frankel method；Alb (white eggs
Content uses Bromocresol green in vain)；TBiL (total bilirubin) content uses sodium benzoate-caffeine colorimetric method, by SHUGUANG HOSPITAL
Clinical laboratory's automatic biochemistry analyzer detection.One-way analysis of variance is carried out using the ANOVA programs in SPSS12.0 softwares, is used in combination
LSD programs are compared two-by-two.Semi-quantitative analysis uses Ridit method statistics.
Experimental result：
(1) before modeling, each group mouse can normal diet, fur gloss, activity is good, and reaction is quick, and body weight increases by the phase.
After carbon tetrachloride modeling, the obvious activity decrease of mouse in addition to blank group, fur is dim, and finds that model group mouse has activity to subtract
Slowly, instability of gait phenomenon.
(2) liver visually observes
Naive mice liver visually observes that color and luster is scarlet, matter is soft, clear-cut margin；Model group mouse edge is slightly blunt, liver
Slightly swelling.Remaining each group liver substantially changes unobvious.
(3) mouse liver microexamination
Naive mice liver leaflet structure is normal, and liver plate is arranged radially, and liver cell form is normal.Model group liver plate
Arrangement disorder, liver cell are generally denatured, and water sample becomes and the fat based on vesicle becomes obvious, part of hepatocytes balloon sample
Become, denaturation liver cell is located at the band of liver acinus I and III band more, and severe patient II also has denaturation with liver cell.There are the point-like being dispersed in or stove
Shape necrosis and cell infiltration.The composition administration group of embodiment 1, hepatocellular injury situation is light compared with model group, is in the small of I band
Bubble property fat becomes or the water sample of II band becomes, and balloon sample becomes and meronecrosis is rare, and sinus hepaticus is narrow.The composition administration group of embodiment 3,
Liver damages situation：Liver cell fat change person is how seldom in I band, the balloon sample change liver cell observed in II band.2 groups of embodiment
Compound administration group, the steatosis of liver cell and the ballooning degeneration of liver cell are seldom, and necrosis of liver cells situation has no, are each group
Middle pathological change most the lighter.
(4) each group mouse liver cell deformation semi-quantitative analysis
Table 2：Each group degeneration of liver cells degree
Note：Compared with blank group, * * *：P ＜ 0.001；
Compared with model group, ##：P ＜ 0.01.
Table 2 shows that naive mice liver cell is not denatured.The denaturation of model group mouse liver cell is serious, mostly IV grade, with
Normal group comparing difference is significantly (P ＜ 0.001).The composition administration group of embodiment 1, degeneration of liver cells (P ＜ light compared with model group
0.01).The composition administration group of embodiment 3, degeneration of liver cells is substantially light (P ＜ 0.01) compared with model group, liver cell I, II, III grade
The number of cases of denaturation occupies the majority, without the sample of IV grade of denaturation.The composition administration group of embodiment 2, degeneration of liver cells degree is most light,
I, II grade is concentrated on, without the sample of IV grade of denaturation.
(5) the observation CCl of the therapeutic action to acute hepatic injury mice and hepatotoxic biochemical indicator4After injection, model
Group mouse ALT and AST activity and TBiL contents significantly raise, and have significant difference (equal P ＜ 0.01) compared with blank group.Implement
ALT the and AST activity of the composition administration group of example 1 also raises, but lower than model group, there is significant difference (P ＜ 0.05, P ＜
0.01), the rise of TBiL contents is similar to model group, and Alb content reduces similar to model group.The composition administration group of embodiment 3
ALT and AST activity is obvious low (P ＜ 0.001, P ＜ 0.01) compared with model group group, TBiL contents rise (P ＜ 0.05), Alb
Content reduces (P ＜ 0.05).The composition administration group of embodiment 2 can effectively suppress CCl4Caused transaminase activity rise,
ALT and AST activity is obvious low (P ＜ 0.05, P ＜ 0.01) compared with model group, and pathological observation displays that this group of degeneration of liver cells
Degree is lighter than model group.
The experimental result of blank group, model group and each experimental group is shown in Table 3.
The serum alt of table 3, AST contents, the result of hepatic pathology detection
Compared with blank group, * represents P<0.05；* represents P<0.01
Compared with model group, # represents P<0.05## represents P<0.01.
Carbon tetrachloride is the chemical substance of long recognized duplication liver damage animal model, and many researchs think four
The hepatic injury that chlorination carbon replicates can simulate chemical damage, its symptom showed, Liver function grade index, hepatic pathology
Change has similitude with virus hepatitis.In this experiment, animal disposably gives CCl4Afterwards, compared with blank group, ALT
Significantly raised with AST activity and TBiL contents, degeneration of liver cells is serious, model group significant difference (equal P ＜ compared with normal group
0.01).The detection of model group hepatic pathology is mainly shown as liver cell fatty degeneration, illustrates chemical liver injury success；With model
Compare, ALT activity significantly reduces in liver homogenate in the composition administration group of embodiment 1~3, AST activity significantly reduces, TBiL contents
Significantly reduce, degeneration of liver cells degree is decreased obviously.Judged according to result, the present composition has certain alleviation chemically property
The effect of hepatic injury.
The explanation of above example is only intended to help the method and its core concept for understanding the present invention.It should be pointed out that pair
For those skilled in the art, under the premise without departing from the principles of the invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.
The foregoing description of the disclosed embodiments, professional and technical personnel in the field are enable to realize or using the present invention.
A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one
The most wide scope caused.
Claims (10)
1. a kind of Chinese medicine composition for alleviating chemical damage, is made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 2 ~ 6；
The parts by weight of ganoderma lucidum 2 ~ 6；
The parts by weight of centella 1 ~ 5；
The parts by weight of rhodiola root 1 ~ 5；
The parts by weight of radix glycyrrhizae 1 ~ 3；
The parts by weight of Turmeric P.E 0.1 ~ 1.
2. composition according to claim 1, it is characterised in that be made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 2, the parts by weight of ganoderma lucidum 2, the parts by weight of centella 1, the parts by weight of rhodiola root 1, the parts by weight of radix glycyrrhizae 1, turmeric extraction
The parts by weight of thing 0.1.
3. composition according to claim 1, it is characterised in that be made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 6, the parts by weight of ganoderma lucidum 6, the parts by weight of centella 5, the parts by weight of rhodiola root 5, the parts by weight of radix glycyrrhizae 3, turmeric extraction
The parts by weight of thing 1.
4. composition according to claim 1, it is characterised in that be made up of the component of following parts by weight：
The parts by weight of the root of kudzu vine 4, the parts by weight of ganoderma lucidum 4, the parts by weight of centella 3, the parts by weight of rhodiola root 3, the parts by weight of radix glycyrrhizae 2, turmeric extraction
The parts by weight of thing 0.4.
5. according to the composition described in claim 1 ~ 4 any one, it is characterised in that the preparation method of the Turmeric P.E
For：
Taking turmeric to crush, extracted 1 ~ 5 time with 50% ethanol of 8 ~ 12 times of amounts, 2 ~ 5h, 60 DEG C of Extracting temperature, merges extract solution every time,
Filtration, the mass ratio for being concentrated into turmeric and dry cream is 10：1, obtain Turmeric P.E.
6. a kind of preparation method for the Chinese medicine composition for alleviating chemical damage, comprises the following steps：
（A）By the parts by weight of the root of kudzu vine 2 ~ 6, the parts by weight of ganoderma lucidum 2 ~ 6, the parts by weight of centella 1 ~ 5, the parts by weight of rhodiola root 1 ~ 5 and radix glycyrrhizae 1 ~ 3
Parts by weight mixing, the water for adding 6 ~ 20 times of amounts extract 1 ~ 4 time as Extraction solvent, after filtering, obtain filtrate；
（B）It is 1.0 ~ 1.3 g/ml that the filtrate decompression is concentrated into concentrated solution density, after spray drying, obtains material；
（C）The material is mixed with the parts by weight of Turmeric P.E 0.1 ~ 1, the Chinese traditional medicine composition for the chemical damage that is eased
Thing.
7. preparation method according to claim 6, it is characterised in that the temperature of the extraction is 90 ~ 100 DEG C, extraction
Time is 0.5 ~ 3 hour.
8. composition made from preparation method according to claim 6.
9. a kind of Chinese medicine preparation for treating chemical damage, it is characterised in that by any one of claim 1 ~ 5 or claim
Chinese medicine composition and pharmaceutically acceptable auxiliary material described in 8 are made.
10. the Chinese medicine composition as described in claim 1 ~ 4 any one is in the health products for alleviating chemical damage are prepared
Application.
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| CN107495041B (en) * | 2017-09-23 | 2020-11-20 | 春秋扶正(广东)健康管理有限责任公司 | Functional ganoderma lucidum beverage and preparation method thereof |
| CN108339051A (en) * | 2018-05-03 | 2018-07-31 | 云南彝山天宝生物医药有限公司 | A kind of drug that treating chemical damage, preparation method and applications |
| CN111714539B (en) * | 2019-03-22 | 2022-04-19 | 江苏康缘药业股份有限公司 | Liver-protecting composition and preparation method and application thereof |
| CN111493318A (en) * | 2020-04-26 | 2020-08-07 | 众生健康(广东)科技有限公司 | A composition containing curcumin for relieving hangover and protecting liver, and its preparation method |
| CN111671792B (en) * | 2020-06-28 | 2022-05-17 | 瑞普（天津）生物药业有限公司 | Traditional Chinese medicine composition for preventing and treating liver injury and preparation method thereof |
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