CN105994979A - Functional ryegrass extract and application thereof in biological feed - Google Patents
Functional ryegrass extract and application thereof in biological feed Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a functional ryegrass extract and application thereof in biological feed. The functional ryegrass extract is prepared through the following methods that 1, dry ryegrass and licorice roots are mixed, crushed and subjected to heat reflux extraction with a 60%-70% ethanol solution, extracting solutions are mixed and concentrated until no alcohol smell exists, and ethanol extraction and concentration liquid is obtained, wherein the weight ratio of the ryegrass to licorice roots is (9-11):1; 2, the ethanol extraction and concentration liquid is diluted with water, extracted with petroleum ether, ethyl acetate and water-saturated n-butyl alcohol sequentially and then subjected to concentration under reduced pressure to obtain a petroleum ether extract, an ethyl acetate extract and an n-butyl alcohol extract respectively; 3, the n-butyl alcohol extract is dissolved with water, filtering is conducted, active ingredients are enriched with AB-8 type macroporous resin, 8-12 column volumes are washed with 5%-15% ethyl alcohol, 13-17 column volumes are eluted with 65%-75% ethyl alcohol, 65%-75% ethyl alcohol eluent is collected, concentration under reduced pressure is conducted, spray drying is conducted, and the functional ryegrass extract is obtained. The functional ryegrass extract can significantly improve the growth performance of cattle.
Description
Technical field
The present invention relates to the application in biological feedstuff of the functional plants extract, be specifically related to a kind of functional rye grass extract
And the application in biological feedstuff.
Background technology
Rye grass is perennial plant, stalk height 30-90 centimetre, and matter that base portion joint is taken root is soft.The tip of a leaf is about 2 millimeters；Blade is soft
Soft, have microtriche, sometimes have auricle.Fringe shape spike is upright or the most curved；Rachilla is smooth without hair；Grain husk lanceolar, edge is narrow
Film quality；Lemma Long Circle, herbaceous stem, smooth, top is without awns；The raw short cilium of two ridges.Caryopsis length is about wide 3 times.Flowering fruit bearing stage
The 5-7 month.Rye grass is the good forage of the universal introducing and planting in various places, is born in Meadow Pasture, and roadside wetland is common.It is distributed widely in
Area, Kashmir, Pakistan, Europe, warm temperate zone, Asia, the north, Africa.
Radix Glycyrrhizae is called: state is old, Herba Hedyotis cantonensis, Glycyrrhiza uralensis Fisch., sweet root, pulse family, Glycyrrhiza perennial herb, root and root stock
Sturdy, it is a kind of tonification Chinese herbal medicine.A kind of medicine good to human body, medicinal part is root and rhizome, and Medicinal Materials Characters root is cylinder
Shape, long 25～100 centimetres, diameter 0.6～3.5 centimetres.Crust degree of tightness differs, surface red brown or taupe brown.Rhizome is circle
Cylindricality, there is bud trace on surface, has marrow in the middle part of section.Feeble QI, taste is sweet and special.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity
Abdomen etc..Happiness is dark moist, sunshine the low dry climate of long temperature.Radix Glycyrrhizae is grown in desert steppe arid, semiarid, sand more
Unconcerned edge and loess hill area.Root and root stock hyoscine.
At present, not yet have been reported that the extract of rye grass and Radix Glycyrrhizae mixture can improve the growth performance of domestic animal.
Summary of the invention
It is an object of the invention to provide a kind of functional rye grass extract, this functional rye grass extract adds biological feedstuff to
The growth performance of cattle can be significantly improved after in.
Above-mentioned purpose is achieved by following technical solution:
A kind of functional rye grass extract, is prepared via a method which: dry rye grass and Radix Glycyrrhizae are mixed by (a), pulverize,
With 60～70% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () will
Step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 5～15% alcohol flushing 8～12 column volumes removings
Big polar component, then with 65～75% ethanol elution 13～17 column volumes, collection 65～75% ethanol elution, concentrating under reduced pressure,
It is spray-dried and get final product；Wherein, rye grass is 9～11:1 with the weight ratio of Radix Glycyrrhizae.
Further, described functional rye grass extract is prepared via a method which: (a) is by dry rye grass and Radix Glycyrrhizae
Mixing, pulverizes, with 65% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 10 column volumes of 10% alcohol flushing
Remove big polar component, then with 15 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure, spraying
It is drying to obtain；Wherein, rye grass is 10:1 with the weight ratio of Radix Glycyrrhizae.
Further, described functional rye grass extract is prepared via a method which: (a) is by dry rye grass and Radix Glycyrrhizae
Mixing, pulverizes, with 60% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 12 column volumes of 5% alcohol flushing
Remove big polar component, then with 17 column volumes of 65% ethanol elution, collect 65% ethanol elution, concentrating under reduced pressure, spraying
It is drying to obtain；Wherein, rye grass is 9:1 with the weight ratio of Radix Glycyrrhizae.
Further, described functional rye grass extract is prepared via a method which: (a) is by dry rye grass and Radix Glycyrrhizae
Mixing, pulverizes, with 70% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 8 column volumes of 15% alcohol flushing
Remove big polar component, then with 13 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure, spraying
It is drying to obtain；Wherein, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae.
The application in terms of improving domestic animal growth performance of the above-mentioned functions rye grass extract.
A kind of biological feedstuff, containing above-mentioned functional rye grass extract.
The application in terms of improving domestic animal growth performance of the above-mentioned biological feedstuff.
Advantages of the present invention:
The functional rye grass extract that the present invention provides can significantly improve the growth performance of cattle, compared with prior art has prominent
The substantive distinguishing features gone out and significantly progress.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
In following embodiment, rye grass takes herb；Radix Glycyrrhizae takes its root and rhizome, dries.
Embodiment 1: the preparation of functional rye grass extract
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 65% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 15 column volumes of 70% ethanol elution, collect
70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 10:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 2: the preparation of functional rye grass extract
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 60% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 12 column volumes of 5% alcohol flushing, then with 17 column volumes of 65% ethanol elution, collect
65% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 9:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 3: the preparation of functional rye grass extract
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 8 column volumes of 15% alcohol flushing, then with 13 column volumes of 75% ethanol elution, collect
75% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 4: the preparation of functional rye grass extract
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 65% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 15 column volumes of 70% ethanol elution, collect
70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 9:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 5: the preparation of functional rye grass extract
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 65% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 15 column volumes of 70% ethanol elution, collect
70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 6: the contrast of embodiment 1, rye grass is 8:1 with the weight ratio of Radix Glycyrrhizae
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 65% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 15 column volumes of 70% ethanol elution, collect
70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 8:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 7: the contrast of embodiment 1, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae
A dry rye grass and Radix Glycyrrhizae are mixed by (), pulverize, with 65% ethanol solution circumfluence distillation, united extraction liquid is dense
It is reduced to obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used successively
Petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, ethyl acetate extraction
Take thing and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, become by AB-8 type macroporous resin enrichment activity
Point, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 15 column volumes of 70% ethanol elution, collect
70% ethanol elution, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae.
Embodiment 8: effect example, the impact on cattle growth performance
One, materials and methods
1, material: prepare rye grass extract 1～7 by embodiment 1～7.
2, method
2.1 experimental animals and packet
Select 3 monthly age Shaanxi cattle 64, be randomly divided into 8 groups, often group 8, male and female half and half.Matched group uses Basic drawing
Feed.Test group 1～7 adds the rye grass of embodiment 1～7 preparation of 1400mg/kg respectively on the basis of Basic drawing respectively
Extract 1～7.
2.2 method for breeding
60 days test periods.Use routine to raise and immunity during whole test, free choice feeding, freely drink water.
2.3 test indexs and method
Growth performance: statistics initial weight, end weight, Average weight increasing a day and feed-weight ratio.
3, statistical analysis
Test data uses SPSS20.0 software to carry out variance analysis, and the significance of difference carries out Duncans multiple comparisons, number in table
Value is expressed as Mean ± SD.
Two, result
The results are shown in Table 1.
As shown in Table 1, the Average weight increasing a day of test group 1,4,5 is above matched group, and feed-weight ratio is below matched group, and compares
Group compares significant difference (P ＜ 0.05)；The end weight of test group 6 and 7 and Average weight increasing a day there was no significant difference (P ＞ with matched group
0.05), feed-weight ratio and matched group there was no significant difference (P ＞ 0.05).
The impact on cattle growth performance of table 1 extract
Test group 2 is similar to the effect of test group 4 and test group 5 with the effect of test group 3, without significant difference.
Result shows, the functional rye grass extract that the present invention provides can significantly improve cattle growth performance.
The above-mentioned effect of extract of the present invention is relevant with the weight ratio of rye grass and Radix Glycyrrhizae.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (7)
1. a functional rye grass extract, it is characterised in that be prepared via a method which: (a) is by dry rye grass
Mix with Radix Glycyrrhizae, pulverize, with 60～70% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol without alcohol taste and carry
Take concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water successively
Saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；
C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 5～15% ethanol rush
Wash 8～12 column volumes and remove big polar components, then with 65～75% ethanol elution 13～17 column volumes, collection 65～75% second
Alcohol eluen, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 9～11:1 with the weight ratio of Radix Glycyrrhizae.
Functional rye grass extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry rye grass and Radix Glycyrrhizae are mixed, pulverizes, with 65% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol
Taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
10% alcohol flushing 10 column volume removes big polar component, then with 15 column volumes of 70% ethanol elution, collects 70% ethanol
Eluent, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 10:1 with the weight ratio of Radix Glycyrrhizae.
Functional rye grass extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry rye grass and Radix Glycyrrhizae are mixed, pulverizes, with 60% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol
Taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
5% alcohol flushing 12 column volume removes big polar component, then with 17 column volumes of 65% ethanol elution, collects 65% ethanol and wash
De-liquid, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 9:1 with the weight ratio of Radix Glycyrrhizae.
Functional rye grass extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry rye grass and Radix Glycyrrhizae are mixed, pulverizes, with 70% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol
Taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
15% alcohol flushing 8 column volume removes big polar component, then with 13 column volumes of 75% ethanol elution, collects 75% ethanol and wash
De-liquid, concentrating under reduced pressure, it is spray-dried and get final product；Wherein, rye grass is 11:1 with the weight ratio of Radix Glycyrrhizae.
5. Claims 1 to 4 arbitrary described functional rye grass extract application in terms of improving domestic animal growth performance.
6. a biological feedstuff, it is characterised in that containing the arbitrary described functional rye grass extract of Claims 1 to 4.
7. the application in terms of improving domestic animal growth performance of the biological feedstuff described in claim 6.
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| CN108401984A (en) * | 2018-05-03 | 2018-08-17 | 山东胜伟旅游发展有限公司 | The method for feeding milch goat with the raw rye grass feed of salt |
| CN108887229A (en) * | 2018-05-03 | 2018-11-27 | 山东胜伟旅游发展有限公司 | In the method for the raw rye grass feeding cow of salt |
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| CN103404718A (en) * | 2013-07-13 | 2013-11-27 | 金为强 | Cattle feed |
| CN105147907A (en) * | 2015-10-22 | 2015-12-16 | 淄博夸克医药技术有限公司 | Dasheen flower extract, as well as preparation method and medicinal application thereof |
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| CN101011103A (en) * | 2006-12-11 | 2007-08-08 | 彭心笑 | Use of ryegrass and method for processing pig feed stuff using same |
| CN102845632A (en) * | 2012-08-06 | 2013-01-02 | 王茜 | Novel safe environmentally-friendly healthy pig feed additive |
| CN103404718A (en) * | 2013-07-13 | 2013-11-27 | 金为强 | Cattle feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108401984A (en) * | 2018-05-03 | 2018-08-17 | 山东胜伟旅游发展有限公司 | The method for feeding milch goat with the raw rye grass feed of salt |
| CN108887229A (en) * | 2018-05-03 | 2018-11-27 | 山东胜伟旅游发展有限公司 | In the method for the raw rye grass feeding cow of salt |
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