CN105943746A - Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs - Google Patents
Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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Abstract
The invention discloses a traditional Chinese medicine Chinese crossostepium leaf extract capable of improving production performance of weaned pigs. The Chinese crossostepium leaf extract is prepared by the following method: (a) mixing dried Chinese crossostepium leaves with common curculigo rhizomes, performing crushing, performing hot reflux extraction with an ethanol solution of 85-95%, merging extracting solutions, and performing concentration until alcohol taste does not exist so as to obtain an alcohol extract concentrated solution; (b) diluting the alcohol extract concentrated solution obtained in the step (a) with water, performing extraction by sequentially using petroleum ether, ethyl acetate and water-saturated n-butanol, and performing decompression concentration so as to obtain a petroleum ether extract, an ethyl acetate extract and an n-butanol extract; (c) dissolving the n-butanol extract with water, performing filtration, enriching active components with D101 type macroporous resins, flushing 8-12 column volumes with ethanol of 10-20% so as to remove large polarity components, eluting 14-16 column volumes with ethanol of 85-95%, collecting an ethanol eluent of 85-95%, and performing decompression concentration, wherein the common curculigo rhizomes account for 5-15% of the total weight of the Chinese crossostepium leaves and the common curculigo rhizomes.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Chinese medicine Folium Crossostephii Chinensis extract improving Growth Performance of Weaning Piglets,
This extract can significantly improve the growth performance of ablactational baby pig.
Background technology
Piglet 21 age in days early weaning can improve annual output nest number and the farrowing head number of sow, increases economic efficiency, but early weaning
Can make piglet produce strong stress, often show as that appetite is poor, digestive functional disturbance, diarrhoea, growthing lag etc., i.e. usual institute
" the early weaning syndrome " said.
Folium Crossostephii Chinensis is the leaf of feverfew Hibisci Mutabilis chrysanthemum.Former plant Hibisci Mutabilis chrysanthemum is upright, branch undershrub, high 10～40 centimetres.Leaf is mutual
Raw, tightly gather in Zhi Ding, the narrow shape of falling ovum wedge shape, grow 2～3 centimetres, close by canescence pubescence, top 3～5 fissure or division,
Base portion is long and narrow, and sliver is avette or narrow square circular.Head inflorescence is subsphaeroidal, diameter 4～5 millimeters, is born in the axil of top, has handle,
Cheng Yiding is raw, the raceme of tool leaf；Flower polygamy；Spending of peripheral two row is female, tubulose, 2 or 3 short fissures；The two of middle part
Property, tubulose, 5 short split.Achene has corner angle 5, and apical cap is with short and small, the scale of division.Spring at florescence.There is cultivation China middle and south.
Rhizoma Curculiginis is the rhizome of amrallid Rhizoma Curculiginis.2～April germinate before or 7～JIUYUE Seedling take rhizome when withering, clean, remove
Fibrous root and root head, dry.Or dry after steaming.Former plant Rhizoma Curculiginis is perennial herb.Rhizome extends, long up to 30 centimetres, circle
Column, meat, crust brown；Root is sturdy, meat, and the aerial stem of plant is inconspicuous.Leaf 3～6 roots go out, narrow lanceolar, and long 10～25
Centimetre, tip is tapering, and a kind of sedge subordinate prolongs into handle, expands in sheath shape still further below, long 4～10 centimetres, green white, edge film quality；Leaf
Arteries and veins is obvious, has middle arteries and veins；Two sides dredge growth pubescence, after the most smooth.Flower axil is raw；Bennet length 1～2.5 centimetres, ensconce in sheath；
Flower polygamy, top is male flower, and bottom is hermaphrodite flower；Bract lanceolar, green, film quality, villosity；The diameter about 1 of flower
Casket rice, perianth lower elongate tubulose, it is about 2 centimetres or longer, top 6 is split, sliver lanceolar, long 8～12 millimeters, inner face
Yellow, outside white, there is long pubescence；Stamen 6, filigree is short；Ovary is long and narrow, villosity.Berry is oval, slightly meat,
Being about 1.2 centimetres, tip has beak, villosity, and seed is somewhat bulbous, bright black, has beak, surface to have wavy rill.Flower
Phase 6～August.Wild in Plain wild grass ground Yang Chu or mixed raw in hillside Rhizoma Imperatae and awn winnow basket bone clump.Distribution Jiangsu, Zhejiang, good fortune
Build, Taiwan, Guangdong, Guangxi, Hunan, Hubei, Sichuan, Guizhou, the ground such as Yunnan.Main product in Sichuan, Yunnan, Guizhou,
Additionally, the ground such as Guangdong, Guangxi also produces.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine Folium Crossostephii Chinensis extract improving Growth Performance of Weaning Piglets, this extract can substantially carry
The growth performance of high ablactational baby pig.
Above-mentioned purpose is achieved by following technical solution:
A kind of Chinese medicine Folium Crossostephii Chinensis extract improving Growth Performance of Weaning Piglets, is prepared via a method which: (a) is by dry Folium Crossostephii Chinensis
Mix with Rhizoma Curculiginis, pulverize, with 85～95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol without alcohol taste and carry
Take concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water successively
Saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；
C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 10～20% ethanol
Rinse 8～12 column volumes and remove big polar components, then with 85～95% ethanol elution 14～16 column volumes, collection 85～95%
Ethanol elution, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for the 5%～15% of Folium Crossostephii Chinensis and Rhizoma Curculiginis gross weight.
Further, described Chinese medicine Folium Crossostephii Chinensis extract is prepared via a method which: dry Folium Crossostephii Chinensis and Rhizoma Curculiginis are mixed by (a),
Pulverize, with 90% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 10 column volumes of 15% alcohol flushing
Property composition, then with 15 column volumes of 90% ethanol elution, collect 90% ethanol elution, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for
Folium Crossostephii Chinensis and the 10% of Rhizoma Curculiginis gross weight.
Further, described Chinese medicine Folium Crossostephii Chinensis extract is prepared via a method which: dry Folium Crossostephii Chinensis and Rhizoma Curculiginis are mixed by (a),
Pulverize, with 85% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 12 column volumes of 10% alcohol flushing
Property composition, then with 16 column volumes of 85% ethanol elution, collect 85% ethanol elution, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for
Folium Crossostephii Chinensis and the 5% of Rhizoma Curculiginis gross weight.
Further, described Chinese medicine Folium Crossostephii Chinensis extract is prepared via a method which: dry Folium Crossostephii Chinensis and Rhizoma Curculiginis are mixed by (a),
Pulverize, with 95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；(b)
By step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction,
Concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract is used
Water dissolution, filters, and with D101 type macroporous resin enrichment active component, first removes big pole with 8 column volumes of 20% alcohol flushing
Property composition, then with 14 column volumes of 95% ethanol elution, collect 95% ethanol elution, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for
Folium Crossostephii Chinensis and the 15% of Rhizoma Curculiginis gross weight.
The application in terms of improving Growth Performance of Weaning Piglets of the above-mentioned Chinese medicine Folium Crossostephii Chinensis extract.
Advantages of the present invention:
The Chinese medicine Folium Crossostephii Chinensis method for preparing extractive improving Growth Performance of Weaning Piglets that the present invention provides is simple, can industrialized production；
This extract can significantly improve Growth Performance of Weaning Piglets.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of Folium Crossostephii Chinensis extract
A Folium Crossostephii Chinensis and Rhizoma Curculiginis (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 90% ethanol solution circumfluence distillation
(20L × 3 time), united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained second
Alcohol extraction concentrated solution dilute with water (3L), satisfies with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water successively
The n-butanol extraction (3L × 3 time) of sum, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 15%
10 column volumes of alcohol flushing remove big polar component, then with 15 column volumes of 90% ethanol elution, collect 90% ethanol elution
Liquid, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of Folium Crossostephii Chinensis extract
A Folium Crossostephii Chinensis and Rhizoma Curculiginis (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 85% ethanol solution circumfluence distillation
(20L × 3 time), united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained second
Alcohol extraction concentrated solution dilute with water (3L), satisfies with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water successively
The n-butanol extraction (3L × 3 time) of sum, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 10%
12 column volumes of alcohol flushing remove big polar component, then with 16 column volumes of 85% ethanol elution, collect 85% ethanol elution
Liquid, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of Folium Crossostephii Chinensis extract
A Folium Crossostephii Chinensis that 5kg is dried by () and Rhizoma Curculiginis (accounting for gross weight 15%) mixing, pulverize, with 95% ethanol solution circumfluence distillation,
United extraction liquid (20L × 3 time), is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol
Extract concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturation
N-butyl alcohol (3L × 3 time) extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol
Extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 20%
8 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 95% ethanol elution, collect 95% ethanol elution
Liquid, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, without Rhizoma Curculiginis
A Folium Crossostephii Chinensis that 5kg is dried by () is pulverized, with 90% ethanol solution circumfluence distillation (20L × 3 time), and united extraction liquid,
It is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water (3L),
Successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), subtract
Pressure concentrates, and respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol extract water
Dissolve, filter, with D101 type macroporous resin enrichment active component, first remove big polarity with 10 column volumes of 15% alcohol flushing
Composition, then with 15 column volumes of 90% ethanol elution, collects 90% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 5: the contrast of embodiment 1, Rhizoma Curculiginis accounts for the 20% of gross weight
A Folium Crossostephii Chinensis and Rhizoma Curculiginis (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 90% ethanol solution circumfluence distillation
(20L × 3 time), united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained second
Alcohol extraction concentrated solution dilute with water (3L), satisfies with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water successively
The n-butanol extraction (3L × 3 time) of sum, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and positive fourth
Alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 15%
10 column volumes of alcohol flushing remove big polar component, then with 15 column volumes of 90% ethanol elution, collect 90% ethanol elution
Liquid, concentrating under reduced pressure and get final product.
Embodiment 6: the impact on Growth Performance of Weaning Piglets
One, materials and methods
1, material: embodiment 1～5 is shown in the preparation of extract 1～5.
2, method
2.1 experimental animals and packet
With reference to NRC (1998) preparation Basic drawing.Select 120 healthy (21 ± 1) age in days wean DLY ternarys miscellaneous
Hand over piglet, be randomly divided into 6 groups, often group 20, male and female half and half.Matched group uses Basic drawing to feed.Test group 1～5 is respectively
The extract 1～5 of embodiment 1～5 preparation of 250g/t is added respectively on the basis of Basic drawing.
2.2 method for breeding
30 days test periods.Use routine to raise and immunity during whole test, free choice feeding, freely drink water.
2.3 test indexs and method
Growth performance: statistics initial weight, end weight, Average weight increasing a day and feed-weight ratio.
3, statistical analysis
Test data uses SPSS20.0 software to carry out variance analysis, and the significance of difference carries out Duncans multiple comparisons, number in table
Value is expressed as Mean ± SD.
Two, result
The results are shown in Table 1.
As shown in Table 1, the end weight of test group 1～3 and Average weight increasing a day are above matched group, and feed-weight ratio is below matched group, with right
Significant difference (P ＜ 0.05) is compared according to group；The end weight of test group 4 and 5 and Average weight increasing a day there was no significant difference (P with matched group
＞ 0.05), feed-weight ratio and matched group there was no significant difference (P ＞ 0.05).
The impact on Growth Performance of Weaning Piglets of table 1 extract
Result shows, the extract that the present invention provides can significantly improve the growth performance of ablactational baby pig.
The above-mentioned effect of extract of the present invention is relevant with the addition whether adding Rhizoma Curculiginis and Rhizoma Curculiginis.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (5)
1. the Chinese medicine Folium Crossostephii Chinensis extract improving Growth Performance of Weaning Piglets, it is characterised in that be prepared via a method which: (a)
Dry Folium Crossostephii Chinensis and Rhizoma Curculiginis are mixed, pulverizes, with 85～95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into without alcohol
Taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first uses
10～20% alcohol flushing 8～12 column volumes remove big polar components, then with 85～95% ethanol elution 14～16 column volumes, receipts
Collection 85～95% ethanol elution, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for the 5%～15% of Folium Crossostephii Chinensis and Rhizoma Curculiginis gross weight.
Chinese medicine Folium Crossostephii Chinensis extract the most according to claim 1, it is characterised in that be prepared via a method which: (a) will
The Folium Crossostephii Chinensis being dried and Rhizoma Curculiginis mixing, pulverize, with 90% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain without alcohol taste
To ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, acetic acid successively
Ethyl ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol
Extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 15%
10 column volumes of alcohol flushing remove big polar component, then with 15 column volumes of 90% ethanol elution, collect 90% ethanol elution
Liquid, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for the 10% of Folium Crossostephii Chinensis and Rhizoma Curculiginis gross weight.
Chinese medicine Folium Crossostephii Chinensis extract the most according to claim 1, it is characterised in that be prepared via a method which: (a) will
The Folium Crossostephii Chinensis being dried and Rhizoma Curculiginis mixing, pulverize, with 85% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain without alcohol taste
To ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, acetic acid successively
Ethyl ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol
Extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 10%
12 column volumes of alcohol flushing remove big polar component, then with 16 column volumes of 85% ethanol elution, collect 85% ethanol elution
Liquid, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for the 5% of Folium Crossostephii Chinensis and Rhizoma Curculiginis gross weight.
Chinese medicine Folium Crossostephii Chinensis extract the most according to claim 1, it is characterised in that be prepared via a method which: (a) will
The Folium Crossostephii Chinensis being dried and Rhizoma Curculiginis mixing, pulverize, with 95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into and obtain without alcohol taste
To ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, acetic acid successively
Ethyl ester and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol
Extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 20%
8 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 95% ethanol elution, collect 95% ethanol elution
Liquid, concentrating under reduced pressure；Wherein, Rhizoma Curculiginis accounts for the 15% of Folium Crossostephii Chinensis and Rhizoma Curculiginis gross weight.
5. Claims 1 to 4 arbitrary described Chinese medicine Folium Crossostephii Chinensis extract application in terms of improving Growth Performance of Weaning Piglets.
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| CN106343212A (en) * | 2016-10-12 | 2017-01-25 | 陈秋霞 | Pig feed for improving growth performance of pigs |
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| CN105663521A (en) * | 2016-03-04 | 2016-06-15 | 吴正锋 | Traditional Chinese medicine teasel root extract capable of improving reproductive performance of cow |
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| CN105663521A (en) * | 2016-03-04 | 2016-06-15 | 吴正锋 | Traditional Chinese medicine teasel root extract capable of improving reproductive performance of cow |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106343212A (en) * | 2016-10-12 | 2017-01-25 | 陈秋霞 | Pig feed for improving growth performance of pigs |
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