CN105663521A - Traditional Chinese medicine teasel root extract capable of improving reproductive performance of cow - Google Patents
Traditional Chinese medicine teasel root extract capable of improving reproductive performance of cow Download PDFInfo
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- CN105663521A CN105663521A CN201610125217.4A CN201610125217A CN105663521A CN 105663521 A CN105663521 A CN 105663521A CN 201610125217 A CN201610125217 A CN 201610125217A CN 105663521 A CN105663521 A CN 105663521A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
- A61K36/8884—Arisaema, e.g. Jack in the pulpit
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses traditional Chinese medicine teasel root extract capable of improving the reproductive performance of a cow. The traditional Chinese medicine teasel root extract is prepared by carrying out the following steps: (a) mixing dry teasel root and arisaema rhizomatum, smashing, carrying out heat reflux extraction with a 70-80% ethanol solution, mixing extracting solutions, and concentrating until no alcohol taste is left, so that an ethanol extraction concentrated solution is obtained; (b) diluting the ethanol extraction concentrated solution obtained in the step (a) with water, sequentially extracting with petroleum ether, ethyl acetate and water saturated n-butyl alcohol, and carrying out reduced pressure concentration, so that petroleum ether extract, ethyl acetate extract and n-butyl alcohol extract are obtained respectively; and (c) dissolving the n-butyl alcohol extract with water, filtering, enriching active ingredients with D101 macroporous resin, firstly removing high polar ingredients by washing for 6-10 column volumes with 5-15% ethanol, then eluting for 8-12 column volumes with 70-80% ethanol, collecting 70-80% ethanol eluent, and carrying out reduced pressure concentration, wherein the arisaema rhizomatum accounts for 5-15% of the total weight of the teasel root and arisaema rhizomatum.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Background technology
In the middle of cow feeding process, in cow feedstuff, the nutrient substance such as proteins,vitamins,and minerals lacks or nutrition is unbalanced, causes that cow uteri wall is thinning, uterine contraction weakens, ovary diminishes and is resting state, non-estrus. The trophic level of cow can be improved by feeding Chinese medicine extract and oestrus, ovulate.
Chinese medicine Radix Dipsaci is the root of Radix Dipsaci. 8～October excavates, and cleans silt, removes root head, end pin and radicula, dries in the shade or a heatable brick bed is done. Former plant Radix Dipsaci is perennial herb, high 60～90 centimetres. Root length taper, main root is obvious, or several and give birth to, crust yellowish-brown, have elongated fibrous root. Stem is upright, multi-branched, has rib and shallow slot, raw fine and soft hair, rib is dredged raw seta. Leaf is to life; Basal leaf has long handle, blade pinniform drastic crack, and tip sliver is relatively big, the ashamed point of end of blade, and there is rough sawn tooth at edge; Stem leaf mostly is 3 and splits, and central authorities' sliver is maximum, and oval to ovum shape lanceolar, long 11～13 centimetres, wide 4～6 centimetres, both sides sliver is less, and there is rough sawn tooth at edge, and two sides is by white patch volt pubescence; The leaf 3 of the stem tip splits or full edge, has short handle, and hair is less. Spend little, most, globulate head inflorescence; Phyllary number piece, narrow lanceolar, there are a bract, wealthy obovate outside often spending; Calycule has 4 cognates, close raw pubescence; Calyx shallow discoid has 4 teeth, slightly in ovum shape triangle; Corolla is white or light yellow, has sliver 4 pieces deeper, and corolla pipe base portion is gradually narrow, outside close by lower to long pubescence; Stamen 4, the top being born in corolla pipe, and filigree is elongated, stretches out outside corolla; Gynoecium 1, stigma rod-short and flat. Achene ellipse wedge shape, generally outer had obvious 4 ribs by sepal, filbert. Florescence 8～JIUYUE. Really 9～October of phase. Wild in hill and roadside. It is distributed mainly on the ground such as Sichuan, Hubei, Hunan, Yunnan, Tibet.
Arisaema phallospadix C. Y. Wu is the tuber of aroid Arisaema phallospadix C. Y. Wu. The early spring gathers. Former plant Arisaema phallospadix C. Y. Wu is perennial herb, high 20～40 centimetres.Tuber Long Circle, around has granulation, has most palpus shape root. Raw 1 leaf in tuber top, come into leaves sheath; Petiole meat, has purpura; Blade is bird toe shape, 5 lobules, is just 3 lobules, vanelets oblong or ovum shape lanceolar, long 12～14 centimetres, wide 6～6.5 centimetres, the sharp point of tip, basal circular or wealthy wedge shape, has purpura below. Flower unisexuality, dioecism; Spadix; Tool spathe, the tiny majority of male flower; In the intensive sequence of female flower, ovary 1 Room. Knot berry. It is born in remote mountains grotto mouth. The ground such as distribution Guizhou.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Above-mentioned purpose is achieved by following technical solution:
A kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, it is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70～80% alcoholic solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6～10 column volumes of 5～15% alcohol flushing, again with 8～12 column volumes of 70～80% ethanol elution, collect 70～80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5%～15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 75% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, again with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Above-mentioned Chinese medicine Radix Dipsaci extract is improving the application of cow reproduction aspect of performance.
Advantages of the present invention:
The Chinese medicine Radix Dipsaci extract preparation method of raising cow reproduction performance provided by the invention is simple, can industrialized production; This extract can significantly improve the reproductive performance of cow.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this. Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 70% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, again with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 15%) that 5kg is dried by () mix, and pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid (20L × 3 time), are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), extract with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) successively, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, again with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, without Arisaema phallospadix C. Y. Wu
A Radix Dipsaci that 5kg is dried by () is pulverized, and with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 5: the contrast of embodiment 1, Arisaema phallospadix C. Y. Wu accounts for the 20% of gross weight
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 6: the impact on cow reproduction performance
One, materials and methods
1, test specimen: prepare extract 1～5 according to embodiment 1～5 respectively.
2, method
2.1 experimental animals, packet and method for breeding
Though selecting non-reproductive system disease, non-reproductive system deformity but because malnutrition causes that ovary is the Chinese XIMEN Qin 72 of resting state, non-estrus, being randomly divided into 6 groups, matched group 12, test group 1～5 often organizes 12. Control group fed basis forage, test group 1～5 adds the extract 1～5 of 250g/t respectively respectively on the basis of basis forage.
Test 1 month by a definite date.
2.2 statistical indicators
Respectively organize estrus of cow quantity in the statistical test time limit and oestrus by dosage, calculating each group of estrus of cow rate with oestrusing and joined rate.
Two, result
As can be seen from Table 1, test group 1～3 compared with matched group, estrus of cow rate and oestrus and joined rate and be significantly increased, significant difference (P < 0.05); Test group 4 with 5 compared with matched group, estrus of cow rate and oestrus and joined rate and there was no significant difference (P > 0.05). It is specifically shown in table 1.
Table 1 extract is on estrus of cow rate and oestruses by the impact joining rate
It is shown that extract provided by the invention can significantly improve estrus of cow rate is joined rate with oestrusing.
The above-mentioned effect of extract of the present invention is relevant with the addition whether adding Arisaema phallospadix C. Y. Wu and Arisaema phallospadix C. Y. Wu.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.It will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from essence and the protection domain of technical solution of the present invention.
Claims (5)
1. the Chinese medicine Radix Dipsaci extract improving cow reproduction performance, it is characterized in that, it is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70～80% alcoholic solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6～10 column volumes of 5～15% alcohol flushing, again with 8～12 column volumes of 70～80% ethanol elution, collect 70～80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5%～15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
2. Chinese medicine Radix Dipsaci extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 75% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
3. Chinese medicine Radix Dipsaci extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, again with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
4. Chinese medicine Radix Dipsaci extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
5. the arbitrary described Chinese medicine Radix Dipsaci extract of Claims 1 to 4 is improving the application of cow reproduction aspect of performance.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105943864A (en) * | 2016-06-26 | 2016-09-21 | 林天样 | Traditional Chinese medicine common pecteilis root extract capable of improving production performance of cattle |
| CN105943746A (en) * | 2016-06-26 | 2016-09-21 | 林天样 | Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs |
| CN106038717A (en) * | 2016-06-26 | 2016-10-26 | 林天样 | Traditional Chinese medicine herba corydalis tailensis extract capable of improving production performance of fat pigs |
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| CN105125934A (en) * | 2015-09-28 | 2015-12-09 | 济南邦文医药科技有限公司 | Traditional Chinese medicine composition for treating phlegmatic hygrosis and blood stasis type tubal infertility |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105943864A (en) * | 2016-06-26 | 2016-09-21 | 林天样 | Traditional Chinese medicine common pecteilis root extract capable of improving production performance of cattle |
| CN105943746A (en) * | 2016-06-26 | 2016-09-21 | 林天样 | Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs |
| CN106038717A (en) * | 2016-06-26 | 2016-10-26 | 林天样 | Traditional Chinese medicine herba corydalis tailensis extract capable of improving production performance of fat pigs |
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