CN104222704B - A kind of feed prepared by Threonine Fermentation discarded object and preparation method thereof that utilizes - Google Patents
A kind of feed prepared by Threonine Fermentation discarded object and preparation method thereof that utilizes Download PDFInfo
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- CN104222704B CN104222704B CN201410548107.XA CN201410548107A CN104222704B CN 104222704 B CN104222704 B CN 104222704B CN 201410548107 A CN201410548107 A CN 201410548107A CN 104222704 B CN104222704 B CN 104222704B
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Abstract
The invention belongs to amino acid fermentation liquid waste processing field, a kind of feed that utilizes Threonine Fermentation discarded object to prepare, it is prepared as follows and forms, step 1) threonine fermentation liquid filters and decolouring, step 2) isolation of crystalline and mother liquor, step 3) hydrolysis, step 4) is prepared compound medicinal powder, step 5) is prepared major ingredient, and step 6) is prepared feed. The invention also discloses the preparation method of above-mentioned feed. This feed has effectively utilized Su Ji acid fermentation waste material, greatly reduces Feed Manufacturing cost, and has improved nutritional labeling, has realized and having turned waste into wealth.
Description
Technical field
The invention belongs to amino acid fermentation liquid waste processing field, disclose a kind of feed prepared by Threonine Fermentation discarded object and preparation method thereof that utilizes.
Background technology
Threonine is a kind of essential amino acid, the second limiting amino acid of pig feed and the 3rd limiting amino acid of poultry feed, along with lysine, the extensive use of methionine composite in mixed feed, threonine becomes gradually affects the major limitation of growth of animals or poultry sexual factor. Threonine is mainly used as feed addictive, and its production stage is concentrated, crystallization, fox extracting thallus protein, and the mycoprotein producing in its process and mother liquid disposal difficulty are larger, and cost is higher, is difficult to obtain the byproduct that sexual valence is higher.
In recent years, livestock feed price rises steadily, and makes raiser's benefit year after year low, causes aquaculture very risky, has dampened significantly numerous raisers' enthusiasm. Aquaculture cost each raiser's productivity effect always in left and right, it returns root is that feed cost is too high eventually. Modern's cultivation, cast aside traditional raising completely and be that to use coarse food grain, grain offal, the rotten dish of green grass be main animal foodstuff, and support slowly isotype, now be rely on full price commercial feed to throw something and feed completely and carry out quick raising mostly, a large amount of consumables feed in breeding process. So, the height of feed cost and lowly determine the good and bad of benefit completely. Current full price commercial feed is mostly taking fine fodder as master is equipped with various auxin again, hormone is formed by combining. The medicament residue such as antibiotic, growth hormone in pig feed, significantly declines the quality of pork and mouthfeel, can not meet again the requirement of people to healthy diet.
How developing a kind of feed with low cost and that antibiotic-free hormonal medicaments adds is prior art technical problem urgently to be resolved hurrily.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of animal feed that utilizes Threonine Fermentation waste material to prepare, this feed has effectively utilized amino acid fermentation waste material, reduce Feed Manufacturing cost, realize and having turned waste into wealth, and nonreactive bio-hormone adds, ensure the edible safety of meat products.
For achieving the above object, the present invention is by the following technical solutions:
An animal feed that utilizes Threonine Fermentation waste material to prepare, is characterized in that, described feed is prepared as follows and forms:
Step 1) threonine fermentation liquid filters and decolouring: threonine fermentation liquid, through micro-filtrate membrane filtration degerming, is collected respectively trapped substance and filtered solution; Then filtered solution is proceeded to ultrafiltration membrance filter, collect concentrate and penetrating fluid; Above-mentioned trapped substance and concentrate are merged into feed liquid A for subsequent use; Penetrating fluid is pumped into the bleacher processing of decolouring, add the active carbon that accounts for penetrating fluid quality 1% in bleacher, the temperature of controlling in bleacher is 45 DEG C, after decolouring 30min, removes active carbon obtain feed liquid B with plate-frame filtering;
Step 2) isolation of crystalline and mother liquor: feed liquid B is concentrated into 1/3 of stoste volume, then crystallization, last centrifugal collection threonine crystal and mother liquor through evaporimeter;
Step 3) hydrolysis: feed liquid A is dried, pulverizer is ground into Powdered, then be placed in reactor, add the hydrochloric acid of 6-8mol/L, not have raw material to be as the criterion, at 70 DEG C of temperature, stir hydrolysis 12-16 hour, mixing speed is 100 turn/min, then heating volatilize HCl gas to the pH of solution be 2-3, finally add sodium hydrate regulator solution to pH be 6-7, obtain Freamine Ⅲ;
Step 4) is prepared compound medicinal powder:
A, take respectively 1 part of 12 parts of the Radixs Astragali, 10 parts of honeysuckles, 6 parts of dandelions, 5 parts of radix bupleuri, 3 parts, Radix Glycyrrhizae, 2 parts of Chinese trumpet creepers and the wind-weed according to weight portion, for subsequent use;
B, get the Radix Astragali, honeysuckle, dandelion and radix bupleuri, mix, the water intense fire that adds 3 times of weight boils, and then slow fire boiling 30 minutes is filtered and collected filtrate, is condensed into the medicinal extract that density is 1.1-1.2g/ml; After 70 DEG C of oven dry, be ground into powder, be compound 1;
C, extracting liquorice, Chinese trumpet creeper and the wind-weed, mix, and with 80% alcohol heating reflux 1 hour, filtered and recycled filtrate, filtrate evaporative removal ethanol, then concentrated to obtain density be 1.1-1.2g/ml condensed cream, and crushed after being dried obtains compound 2;
D, compound 1 and compound 2 are mixed, obtain compound medicinal powder;
Step 5) is prepared major ingredient:
Wheat bran, dregs of beans, Chinese sorghum, peanut shell and maize straw are mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turn/min stirs 10min, adds subsequently step 2) mother liquid obtained, 100 turn/min stirrings 24 hours, obtain major ingredient;
Step 6) is prepared feed: compound medicinal powder and Freamine Ⅲ are added in major ingredient, while stirring heating, to moisture be 30-35%(w/w) time stop heating, granulator granulation, dry, pack and get final product.
Preferably, in described step 1), micro-filtration membrane is inorganic ceramic membrane, and molecular cut off is 2000MW, and micro-filtration temperature is 40 DEG C; Milipore filter molecular cut off is 300MW, and ultrafiltration temperature is 40 DEG C; In described step 5), the weight ratio of wheat bran, dregs of beans, Chinese sorghum, peanut shell, maize straw and mother liquor is 5:3:2:1:1:10;
In described step 6), the weight ratio of compound medicinal powder, Freamine Ⅲ and major ingredient is 1-2:30-45:300-500; Described animal can be pig.
The invention also discloses the preparation method of above-mentioned feed.
The beneficial effect that the present invention obtains mainly comprises:
Feed prepared by the present invention has utilized Threonine Fermentation waste material dexterously, and reduction Feed Manufacturing cost, has realized and having turned waste into wealth, and the interpolation of nonreactive bio-hormone, has ensured the edible safety of meat products; In Threonine Fermentation technique of the present invention, save mother liquid disposal process, reduced production costs, realized and having turned waste into wealth, increased substantially the utilization rate of resource; The mycoprotein that is difficult to digestion is hydrolyzed to amino acid profiles by the present invention, and supplemented salinity dexterously, and nutrition is more comprehensive, has improved the appetite of letting animals feed, strengthens digestion, enhances metabolism; The present invention avoids adopting antibiotic and hormone, has added natural traditional Chinese medicine component, adopts different extracting methods to obtain to greatest extent its active ingredient, and compatibility is reasonable, possesses clearing heat and detoxicatingly, improves immunity and prevents and treats effect of pestilence.
Detailed description of the invention
In order to make those skilled in the art person understand better the technical scheme in the application, below in conjunction with the application's specific embodiment, the present invention is described more clearly and completely, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiment. Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
An animal feed that utilizes Threonine Fermentation waste material to prepare, it is prepared as follows and forms:
Step 1) threonine fermentation liquid filters and decolouring: threonine fermentation liquid, through micro-filtrate membrane filtration degerming, is collected respectively trapped substance and filtered solution; Then filtered solution is proceeded to ultrafiltration membrance filter, collect concentrate and penetrating fluid; Above-mentioned trapped substance and concentrate are merged into feed liquid A for subsequent use; Penetrating fluid is pumped into the bleacher processing of decolouring, add the active carbon that accounts for penetrating fluid quality 1% in bleacher, the temperature of controlling in bleacher is 45 DEG C, after decolouring 30min, removes active carbon obtain feed liquid B with plate-frame filtering; Wherein, micro-filtration membrane is inorganic ceramic membrane, and molecular cut off is 2000MW, and micro-filtration temperature is 40 DEG C; Milipore filter molecular cut off is 300MW, and ultrafiltration temperature is 40 DEG C;
Step 2) isolation of crystalline and mother liquor: feed liquid B is concentrated into 1/3 of stoste volume, then crystallization, last centrifugal collection threonine crystal and mother liquor through evaporimeter;
Step 3) hydrolysis: feed liquid A is dried, pulverizer is ground into Powdered, then be placed in reactor, add the hydrochloric acid of 6mol/L, not have raw material to be as the criterion, at 70 DEG C of temperature, stir hydrolysis 16 hours, mixing speed is 100 turn/min, then heating volatilize HCl gas to the pH of solution be 2, finally add sodium hydrate regulator solution to pH be 6, obtain Freamine Ⅲ;
Step 4) is prepared compound medicinal powder:
A, take respectively 1 part of 12 parts of the Radixs Astragali, 10 parts of honeysuckles, 6 parts of dandelions, 5 parts of radix bupleuri, 3 parts, Radix Glycyrrhizae, 2 parts of Chinese trumpet creepers and the wind-weed according to weight portion, for subsequent use;
B, get the Radix Astragali, honeysuckle, dandelion and radix bupleuri, mix, the water intense fire that adds 3 times of weight boils, and then slow fire boiling 30 minutes is filtered and collected filtrate, is condensed into the medicinal extract that density is 1.2g/ml; After 70 DEG C of oven dry, be ground into powder, be compound 1;
C, extracting liquorice, Chinese trumpet creeper and the wind-weed, mix, and adds hot reflux 1 hour with 80% ethanol (v/v), filtered and recycled filtrate, and filtrate evaporative removal ethanol, it is then concentrated that to obtain density be 1.1g/ml condensed cream, crushed after being dried obtains compound 2;
D, compound 1 and compound 2 are mixed, obtain compound medicinal powder;
Step 5) is prepared major ingredient:
Wheat bran, dregs of beans, Chinese sorghum, peanut shell and maize straw are mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turn/min stirs 10min, adds subsequently step 2) mother liquid obtained, 100 turn/min stirrings 24 hours, obtain major ingredient; Wherein, the weight ratio of wheat bran, dregs of beans, Chinese sorghum, peanut shell, maize straw and mother liquor is 5:3:2:1:1:10;
Step 6) is prepared feed: compound medicinal powder and Freamine Ⅲ are added in major ingredient, while stirring heating, to moisture be 30%(w/w) time stop heating, granulator granulation, dry, pack and get final product; Wherein, the weight ratio of compound medicinal powder, Freamine Ⅲ and major ingredient is 1:30:350.
Embodiment 2
An animal feed that utilizes Threonine Fermentation waste material to prepare, it is prepared as follows and forms:
Step 1) threonine fermentation liquid filters and decolouring: threonine fermentation liquid, through micro-filtrate membrane filtration degerming, is collected respectively trapped substance and filtered solution; Then filtered solution is proceeded to ultrafiltration membrance filter, collect concentrate and penetrating fluid; Above-mentioned trapped substance and concentrate are merged into feed liquid A for subsequent use; Penetrating fluid is pumped into the bleacher processing of decolouring, add the active carbon that accounts for penetrating fluid quality 1% in bleacher, the temperature of controlling in bleacher is 45 DEG C, after decolouring 30min, removes active carbon obtain feed liquid B with plate-frame filtering; Wherein, micro-filtration membrane is inorganic ceramic membrane, and molecular cut off is 2000MW, and micro-filtration temperature is 40 DEG C; Milipore filter molecular cut off is 300MW, and ultrafiltration temperature is 40 DEG C;
Step 2) isolation of crystalline and mother liquor: feed liquid B is concentrated into 1/3 of stoste volume, then crystallization, last centrifugal collection threonine crystal and mother liquor through evaporimeter;
Step 3) hydrolysis: feed liquid A is dried, pulverizer is ground into Powdered, then be placed in reactor, add the hydrochloric acid of 8mol/L, not have raw material to be as the criterion, at 70 DEG C of temperature, stir hydrolysis 12 hours, mixing speed is 100 turn/min, then heating volatilize HCl gas to the pH of solution be 3, finally add sodium hydrate regulator solution to pH be 7, obtain Freamine Ⅲ;
Step 4) is prepared compound medicinal powder:
A, take respectively 1 part of 12 parts of the Radixs Astragali, 10 parts of honeysuckles, 6 parts of dandelions, 5 parts of radix bupleuri, 3 parts, Radix Glycyrrhizae, 2 parts of Chinese trumpet creepers and the wind-weed according to weight portion, for subsequent use;
B, get the Radix Astragali, honeysuckle, dandelion and radix bupleuri, mix, the water intense fire that adds 3 times of weight boils, and then slow fire boiling 30 minutes is filtered and collected filtrate, is condensed into the medicinal extract that density is 1.1g/ml; After 70 DEG C of oven dry, be ground into powder, be compound 1;
C, extracting liquorice, Chinese trumpet creeper and the wind-weed, mix, and with 80% alcohol heating reflux 1 hour, filtered and recycled filtrate, filtrate evaporative removal ethanol, then concentrated to obtain density be 1.2g/ml condensed cream, and crushed after being dried obtains compound 2;
D, compound 1 and compound 2 are mixed, obtain compound medicinal powder;
Step 5) is prepared major ingredient:
Wheat bran, dregs of beans, Chinese sorghum, peanut shell and maize straw are mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turn/min stirs 10min, adds subsequently step 2) mother liquid obtained, 100 turn/min stirrings 24 hours, obtain major ingredient; Wherein, the weight ratio of wheat bran, dregs of beans, Chinese sorghum, peanut shell, maize straw and mother liquor is 5:3:2:1:1:10;
Step 6) is prepared feed: compound medicinal powder and Freamine Ⅲ are added in major ingredient, while stirring heating, to moisture be 35%(w/w) time stop heating, granulator granulation, dry, pack and get final product; Wherein, the weight ratio of compound medicinal powder, Freamine Ⅲ and major ingredient is 1:45:500.
Embodiment 3
Feed efficiency test prepared by the present invention:
One, choose 300 and infect the pigs of swine fevers, pig age is between the 2-4 month; Symptoms is: nameless high-fever, and anorexia or disappearance, eye conjunctivitis occurs also has purulent secretion, and nasal cavity permanent current goes out mycopus, has vomiting, has blood in stool; The skin of the basal part of the ear, belly, burst inner side often has many petechial hemorrhages or bright redder point. Mesenteric adenophyma is swollen, often occurs hemorrhagic enteritis, with the button-type ulcer in colorectal mucosa. It is divided into three groups at random, 100 every group; Three groups all adopt qingwen baidu powder treatment, and every 1kg body weight is taken 20g orally, is used in conjunction 7 days.
Experimental group: give feed prepared by the embodiment of the present invention 1; Control group 1: lead to large swine feed in prestige; Control group 2: give embodiment 1 feed, not containing Chinese medicine component.
Efficacy assessment standard: cure: symptom disappears completely, main physico-chemical examination index is normal; Take a turn for the better: each side symptom makes moderate progress compared with before treatment; Without positive effect: each side symptom is all not improved compared with before treatment. Concrete case is added up in table 1:
Table 1
| Group | Number of cases | Cure | Take a turn for the better | Without positive effect | Obvious effective rate |
| Experimental group | 100 | 51 | 34 | 15 | 85％ |
| Control group 1 | 100 | 27 | 35 | 38 | 62% |
| Control group 2 | 100 | 30 | 34 | 36 | 64% |
Two, choose 200 of the piglets of 60 days, be divided into two groups, 100 every group, the feed that wherein experimental group is prepared with the present embodiment 1 is raised, hog grower feed in logical prestige for control group; Two groups of other raising conditions are identical. Raise and detect indices referring to table 2 afterwards in 9 weeks.
Table 2
Conclusion: feed feeding effect prepared by the present invention is better, daily gain and material anharmonic ratio are better than the conventional pig feed in market, and the more commercially available feed of cost is cheap.
What more than enumerate is only best specific embodiment of the present invention. Obviously, the invention is not restricted to above embodiment, can also have many distortion. All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (2)
1. an animal feed that utilizes Threonine Fermentation waste material to prepare, is characterized in that, described feed is prepared as follows and forms:
Step 1) threonine fermentation liquid filters and decolouring: threonine fermentation liquid, through micro-filtrate membrane filtration degerming, is collected respectively trapped substance and filtered solution; Then filtered solution is proceeded to ultrafiltration membrance filter, collect concentrate and penetrating fluid; Above-mentioned trapped substance and concentrate are merged into feed liquid A for subsequent use; Penetrating fluid is pumped into the bleacher processing of decolouring, add the active carbon that accounts for penetrating fluid quality 1% in bleacher, the temperature of controlling in bleacher is 45 DEG C, after decolouring 30min, removes active carbon obtain feed liquid B with plate-frame filtering;
Step 2) isolation of crystalline and mother liquor: feed liquid B is concentrated into 1/3 of stoste volume, then crystallization, last centrifugal collection threonine crystal and mother liquor through evaporimeter;
Step 3) hydrolysis: feed liquid A is dried, pulverizer is ground into Powdered, then be placed in reactor, add the hydrochloric acid of 6-8mol/L, not have raw material to be as the criterion, at 70 DEG C of temperature, stir hydrolysis 12-16 hour, mixing speed is 100 turn/min, then heating volatilize HCl gas to the pH of solution be 2-3, finally add sodium hydrate regulator solution to pH be 6-7, obtain Freamine Ⅲ;
Step 4) is prepared compound medicinal powder:
A, take respectively 1 part of 12 parts of the Radixs Astragali, 10 parts of honeysuckles, 6 parts of dandelions, 5 parts of radix bupleuri, 3 parts, Radix Glycyrrhizae, 2 parts of Chinese trumpet creepers and the wind-weed according to weight portion, for subsequent use;
B, get the Radix Astragali, honeysuckle, dandelion and radix bupleuri, mix, the water intense fire that adds 3 times of weight boils, and then slow fire boiling 30 minutes is filtered and collected filtrate, is condensed into the medicinal extract that density is 1.1-1.2g/ml; After 70 DEG C of oven dry, be ground into powder, be compound 1;
C, extracting liquorice, Chinese trumpet creeper and the wind-weed, mix, and with 80% alcohol heating reflux 1 hour, filtered and recycled filtrate, filtrate evaporative removal ethanol, then concentrated to obtain density be 1.1-1.2g/ml condensed cream, and crushed after being dried obtains compound 2;
D, compound 1 and compound 2 are mixed, obtain compound medicinal powder;
Step 5) is prepared major ingredient:
Wheat bran, dregs of beans, Chinese sorghum, peanut shell and maize straw are mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turn/min stirs 10min, adds subsequently step 2) mother liquid obtained, 100 turn/min stirrings 24 hours, obtain major ingredient;
Step 6) is prepared feed: compound medicinal powder and Freamine Ⅲ are added in major ingredient, while stirring heating, to moisture be 30-35%(w/w) time stop heating, granulator granulation, dry, pack and get final product;
In described step 5), the weight ratio of wheat bran, dregs of beans, Chinese sorghum, peanut shell, maize straw and mother liquor is 5:3:2:1:1:10;
In described step 6), the weight ratio of compound medicinal powder, Freamine Ⅲ and major ingredient is 1-2:30-45:300-500.
2. feed according to claim 1, is characterized in that, in described step 1),
Micro-filtration membrane is inorganic ceramic membrane, and molecular cut off is 2000MW, and micro-filtration temperature is 40 DEG C; Milipore filter molecular cut off is 300MW, and ultrafiltration temperature is 40 DEG C.
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| CN104757273B (en) * | 2015-04-12 | 2017-08-01 | 呼伦贝尔东北阜丰生物科技有限公司 | A kind of technique for preparing granular pattern threonine product |
| CN109275782A (en) * | 2018-05-18 | 2019-01-29 | 山阳县恒桓生物科技有限公司 | A kind of processing method of waste water of mgs |
| CN111066960A (en) * | 2018-10-22 | 2020-04-28 | 通辽市海邻生物科技有限公司 | Method for increasing essential amino acids of meal energy feed |
| CN111066959A (en) * | 2018-10-22 | 2020-04-28 | 内蒙古中润生物科技有限公司 | Method for producing high-quality nucleotide residues by taking corn germ meal as carrier |
| CN111758840A (en) * | 2020-07-10 | 2020-10-13 | 廊坊梅花生物技术开发有限公司 | Feed using threonine mother liquor as raw material and preparation method thereof |
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