CN105943761A - Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows - Google Patents
Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows Download PDFInfo
- Publication number
- CN105943761A CN105943761A CN201610320538.XA CN201610320538A CN105943761A CN 105943761 A CN105943761 A CN 105943761A CN 201610320538 A CN201610320538 A CN 201610320538A CN 105943761 A CN105943761 A CN 105943761A
- Authority
- CN
- China
- Prior art keywords
- extract
- radix dipsaci
- ethanol
- petroleum ether
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
- A61K36/8884—Arisaema, e.g. Jack in the pulpit
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicinal radix dipsaci extract capable of improving the reproductive performance of cows. A preparation method of the radix dipsaci extract comprises the following steps: (a) mixing dried radix dipsaci and tuber arisaematis rhizomati, smashing the mixed dried radix dipsaci and tuber arisaematis rhizomati, carrying out heat reflux extraction by adopting 70-80% of ethanol solution, mixing the extracting solutions, and concentrating the mixed extracting solution till no alcohol smell exist, thus obtaining an ethanol extraction concentrated solution; (b) diluting the ethanol extraction concentrated solution obtained in the step (a) by using water, carrying out extraction by adopting petroleum ether, ethyl acetate and water-saturated n-butyl alcohol, and carrying out vacuum concentration to respectively obtain petroleum ether extract, ethyl acetate extract and n-butyl alcohol extract; and (c) dissolving the n-butyl alcohol extract by adopting water, filtering the obtained solution, gathering active ingredients by adopting D101 type macroporous resin, namely, flushing 6-10 column volumes by adopting 5-15% of alcohol to remove large polar components, eluting 8-12 column volumes by adopting 70-80% of alcohol, collecting the eluant of 70-80% of ethyl alcohol, and carrying out vacuum concentration, wherein the weight of tuber arisaematis rhizomati accounts for 5%-15% of the total weight of radix dipsaci and tuber arisaematis rhizomati.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Background technology
In the middle of cow feeding process, in cow feedstuff, the nutrient substance such as proteins,vitamins,and minerals lacks or nutrition is unbalanced, cause that cow uteri wall is thinning, uterine contraction weakens, ovary diminishes and in resting state, non-estrus.The trophic level of cow can be improved by feeding Chinese medicine extract and oestrus, ovulate.
Chinese medicine Radix Dipsaci is the root of Radix Dipsaci.8 ~ October excavates, and cleans silt, removes root head, end pin and radicula, dries in the shade or a heatable brick bed is done.Former plant Radix Dipsaci is perennial herb, high 60 ~ 90 centimetres.Root length taper, main root is obvious, or several and give birth to, crust yellowish-brown, have elongated fibrous root.Stem is upright, multi-branched, tool rib and shallow slot, raw fine and soft hair, and rib is dredged raw seta.Leaf is to life;Basal leaf has long handle, blade pinniform drastic crack, and tip sliver is relatively big, and the ashamed point of end of blade, there is rough sawn tooth at edge;Stem leaf mostly is 3 and splits, and central authorities' sliver is maximum, and oval to ovum shape lanceolar, long 11 ~ 13 centimetres, wide 4 ~ 6 centimetres, both sides sliver is less, and there is rough sawn tooth at edge, and two sides is by white patch volt pubescence;The leaf 3 of the stem tip splits or full edge, has short handle, and hair is less.Spend little, most, globulate head inflorescence;Phyllary number piece, narrow lanceolar, there are a bract, wealthy obovate outside often spending;Calycule has 4 cognates, close raw pubescence;Calyx shallow discoid has 4 teeth, slightly in ovum shape triangle;Corolla is white or light yellow, has 4 pieces of deeper slivers, and corolla pipe base portion is the narrowest, outside close by lower to long pubescence;Stamen 4, the top being born in corolla pipe, and filigree is elongated, stretches out outside corolla;Gynoecium 1, stigma rod-short and flat.Achene ellipse wedge shape, outer had obvious 4 ribs by sepal, filbert.Florescence 8 ~ JIUYUE.Really 9 ~ October of phase.Wild in hill and roadside.It is distributed mainly on the ground such as Sichuan, Hubei, Hunan, Yunnan, Tibet.
Arisaema phallospadix C. Y. Wu is the tuber of aroid Arisaema phallospadix C. Y. Wu.The early spring gathers.Former plant Arisaema phallospadix C. Y. Wu is perennial herb, high 20 ~ 40 centimetres.Tuber Long Circle, around has granulation, has most palpus shape root.Raw 1 leaf in tuber top, has long sheath;Petiole meat, has purpura;Blade is bird toe shape, 5 lobules, is just 3 lobules, vanelets oblong or ovum shape lanceolar, long 12 ~ 14 centimetres, wide 6 ~ 6.5 centimetres, the sharp point of tip, and basal circular or wealthy wedge shape have purpura below.Flower unisexuality, dioecism;Spadix;Tool spathe, the tiny majority of male flower;In the intensive sequence of female flower, ovary 1 Room.Knot berry.It is born in remote mountains grotto mouth.The ground such as distribution Guizhou.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Above-mentioned purpose is achieved by following technical solution:
A kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70 ~ 80% ethanol solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 ~ 10 column volumes of 5 ~ 15% alcohol flushing, again with 8 ~ 12 column volumes of 70 ~ 80% ethanol elution, collect 70 ~ 80% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% ~ 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 75% ethanol solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Above-mentioned Chinese medicine Radix Dipsaci extract is improving the application of cow reproduction aspect of performance.
Advantages of the present invention:
The Chinese medicine Radix Dipsaci extract preparation method improving cow reproduction performance that the present invention provides is simple, can industrialized production;This extract can significantly improve the reproductive performance of cow.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, technical scheme can be modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 8 column volumes big polar components of removing of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 70% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 10 column volumes big polar components of removing of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of Radix Dipsaci extract
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 15%) that 5kg is dried by () mix, and pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid (20L × 3 time), are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 6 column volumes big polar components of removing of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, without Arisaema phallospadix C. Y. Wu
A Radix Dipsaci that 5kg is dried by () is pulverized, and with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste;B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 8 column volumes big polar components of removing of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 5: the contrast of embodiment 1, Arisaema phallospadix C. Y. Wu accounts for the 20% of gross weight
A Radix Dipsaci and Arisaema phallospadix C. Y. Wu (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste;B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first with 8 column volumes big polar components of removing of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 6: the impact on cow reproduction performance
One, materials and methods
1, test specimen: prepare extract 1 ~ 5 according to embodiment 1 ~ 5 respectively.
2, method
2.1 experimental animals, packet and method for breeding
Though selecting non-reproductive system disease, non-reproductive system deformity but because malnutrition causes ovary to be the Chinese XIMEN Qin 72 of resting state, non-estrus, being randomly divided into 6 groups, matched group 12, test group 1 ~ 5 often organizes 12.Control group fed basis forage, test group 1 ~ 5 adds the extract 1 ~ 5 of 250g/t respectively on the basis of the forage of basis respectively.
Test 1 month by a definite date.
2.2 statistical indicator
Respectively organize estrus of cow quantity in the statistical test time limit and oestrus by dosage, calculating each group of estrus of cow rate with oestrusing and joined rate.
Two, result
As can be seen from Table 1, test group 1 ~ 3 compared with matched group, estrus of cow rate and oestrus and joined rate and be significantly increased, significant difference (P < 0.05);Test group 4 with 5 compared with matched group, estrus of cow rate and oestrus and joined rate and there was no significant difference (P > 0.05).Specifically it is shown in Table 1.
Estrus of cow rate is affected by joining rate by table 1 extract with oestrusing
Result shows, the extract that the present invention provides can significantly improve estrus of cow rate and be joined rate with oestrusing.
The above-mentioned effect of extract of the present invention is relevant with the addition whether adding Arisaema phallospadix C. Y. Wu and Arisaema phallospadix C. Y. Wu.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.It will be understood by those within the art that, technical scheme can be modified or equivalent, without deviating from essence and the protection domain of technical solution of the present invention.
Claims (5)
1. the Chinese medicine Radix Dipsaci extract improving cow reproduction performance, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70 ~ 80% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 ~ 10 column volumes of 5 ~ 15% alcohol flushing, again with 8 ~ 12 column volumes of 70 ~ 80% ethanol elution, collect 70 ~ 80% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% ~ 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Chinese medicine Radix Dipsaci extract the most according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 75% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Chinese medicine Radix Dipsaci extract the most according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Chinese medicine Radix Dipsaci extract the most according to claim 1, it is characterised in that be prepared via a method which: dry Radix Dipsaci and Arisaema phallospadix C. Y. Wu are mixed by (a), pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste;B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract;C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure;Wherein, Arisaema phallospadix C. Y. Wu accounts for the 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
5. the arbitrary described Chinese medicine Radix Dipsaci extract of claim 1 ~ 4 is improving the application of cow reproduction aspect of performance.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610320538.XA CN105943761A (en) | 2016-05-16 | 2016-05-16 | Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610320538.XA CN105943761A (en) | 2016-05-16 | 2016-05-16 | Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105943761A true CN105943761A (en) | 2016-09-21 |
Family
ID=56911616
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610320538.XA Withdrawn CN105943761A (en) | 2016-05-16 | 2016-05-16 | Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105943761A (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105560669A (en) * | 2016-03-22 | 2016-05-11 | 吴正锋 | Traditional Chinese medicine teasel root extract for improving reproductive performance of cows |
-
2016
- 2016-05-16 CN CN201610320538.XA patent/CN105943761A/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105560669A (en) * | 2016-03-22 | 2016-05-11 | 吴正锋 | Traditional Chinese medicine teasel root extract for improving reproductive performance of cows |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105560669A (en) | Traditional Chinese medicine teasel root extract for improving reproductive performance of cows | |
CN105661069A (en) | Feed additive for improving swine reproductive performance and preparation method thereof | |
CN105248900A (en) | Breeding method for backup boars and sows | |
CN105663521A (en) | Traditional Chinese medicine teasel root extract capable of improving reproductive performance of cow | |
CN104366076A (en) | Meat duck feed | |
CN104222704B (en) | A kind of feed prepared by Threonine Fermentation discarded object and preparation method thereof that utilizes | |
CN105079308A (en) | Common rockvine herb extract as well as preparation method and pharmaceutical application of extract | |
CN102550827B (en) | Feed additive stimulating cows to produce milk | |
CN104206710A (en) | Traditional Chinese medicinal feed additive containing N-carbamylglutamic acid, and its application | |
CN105147907A (en) | Dasheen flower extract, as well as preparation method and medicinal application thereof | |
CN105943761A (en) | Traditional Chinese medicinal radix dipsaci extract capable of improving reproductive performance of cows | |
CN103721008A (en) | Traditional Chinese medicine veterinary drug for treating out of heat of livestock and preparation method of traditional Chinese medicine veterinary drug | |
CN103705627A (en) | Lactation promoting preparation for postpartum mink, preparation method and application thereof | |
CN107864924A (en) | A kind of cultural method for improving porcupine survival rate | |
CN105994979A (en) | Functional ryegrass extract and application thereof in biological feed | |
CN106038717A (en) | Traditional Chinese medicine herba corydalis tailensis extract capable of improving production performance of fat pigs | |
CN107691847A (en) | A kind of green porkling feed and preparation method thereof | |
Jankowska et al. | Effect of extended lactations on milk and reproductive performance of cows | |
CN105943746A (en) | Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs | |
CN112294912A (en) | Important estrus-promoting formula for sow mating and preparation method thereof | |
CN102100730A (en) | Traditional Chinese medicine for improving poultry immunity | |
CN105851526A (en) | Compound feed for meat ducks | |
CN105394441A (en) | Zoea freshwater shrimp feed and preparation method thereof | |
CN111514182A (en) | Lactation promoting powder for livestock and preparation method thereof | |
CN105961852A (en) | Functional mulberry leaf extract and application thereof in biological feed |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WW01 | Invention patent application withdrawn after publication | ||
WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160921 |