CN105560669A - Traditional Chinese medicine teasel root extract for improving reproductive performance of cows - Google Patents
Traditional Chinese medicine teasel root extract for improving reproductive performance of cows Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
- A61K36/8884—Arisaema, e.g. Jack in the pulpit
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a traditional Chinese medicine teasel root extract for improving reproductive performance of cows. The teasel root extract is prepared by the following methods: (a) mixing dried teasel root with arisaema rhizomatum, crushing the mixture, carrying out heat reflux extraction with 70%-80% ethanol solution, merging an extract solution, and concentrating the extract solution until no alcoholic smell to obtain an ethanol-extracted and concentrated solution; (b) diluting the ethanol-extracted and concentrated solution obtained in the step (a) with water, carrying out extraction with petroleum ether, ethyl acetate and water-saturated n-butyl alcohol in order, and carrying out vacuum concentration to obtain a petroleum ether extract, an ethyl acetate extract and an n-butyl alcohol extract respectively; and (c) dissolving the n-butyl alcohol extract with water, carrying out filtration, gathering active ingredients with D101 macroporous resin, washing 6-10 column volumes with 5%-15% ethanol to remove a high-polarity component, then eluting 8-12 column volumes with 70%-80% ethanol, collecting 70%-80% ethanol eluent and carrying out vacuum concentration, wherein the arisaema rhizomatum accounts for 5-15% of total weight of the teasel root and the arisaema rhizomatum.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Background technology
In the middle of cow feeding process, in cow feedstuff the nutrient substance such as proteins,vitamins,and minerals lack or nutrition unbalanced, cause that cow uteri wall is thinning, uterine contraction weakens, ovary diminishes and be resting state, non-estrus.The trophic level of cow can be improved by Chinese medicine extract of feeding and oestrus, ovulate.
Chinese medicine Radix Dipsaci is the root of Radix Dipsaci.8 ~ October excavates, and cleans silt, and removing root head, end pin and radicula, dry in the shade or a heatable brick bed is done.Former plant Radix Dipsaci is perennial herb, high 60 ~ 90 centimetres.The long taper of root, main root is obvious, or several are also raw, crust yellowish-brown, the elongated fibrous root of tool.Stem is upright, multi-branched, tool rib and shallow slot, raw fine and soft hair, rib is dredged raw seta.Leaf is to life; Basal leaf has long handle, blade pinniform drastic crack, and tip sliver is comparatively large, the ashamed point of end of blade, and there is rough sawn tooth at edge; Stem leaf mostly is 3 and splits, and central sliver is maximum, and oval to ovum shape lanceolar, long 11 ~ 13 centimetres, wide 4 ~ 6 centimetres, both sides sliver is less, and there is rough sawn tooth at edge, and volt pubescence is pasted by white in two sides; The leaf 3 of the stem tip splits or full edge, and tool short handle, hair is less.Spend little, most, globulate head inflorescence; Phyllary number piece, narrow lanceolar, has a bract, wealthy obovate outside often spending; Calycule tool 4 cognate, close raw pubescence; Calyx shallow discoid tool 4 tooth, slightly in ovum shape triangle; Corolla white or light yellow, the sliver that tool 4 pieces is darker, corolla pipe base portion is gradually narrow, outside close by lower to long pubescence; Stamen 4, the top being born in corolla pipe, filigree is elongated, stretches out outside corolla; Gynoecium 1, stigma rod-short and flat.The oval wedge shape of achene, usually outer had obvious 4 ribs by sepal, filbert.Florescence 8 ~ JIUYUE.Really 9 ~ October of phase.Wild in hill and roadside.Mainly be distributed in the ground such as Sichuan, Hubei, Hunan, Yunnan, Tibet.
Arisaema phallospadix C. Y. Wu is the tuber of aroid Arisaema phallospadix C. Y. Wu.The early spring gathers.Former plant Arisaema phallospadix C. Y. Wu is perennial herb, high 20 ~ 40 centimetres.Tuber Long Circle, has granulation around, has most palpus shape root.Raw 1 leaf in tuber top, come into leaves sheath; Petiole meat, has purpura; Blade is bird toe shape, 5 lobules, is just 3 lobules, vanelets oblong or ovum shape lanceolar, and long 12 ~ 14 centimetres, wide 6 ~ 6.5 centimetres, the sharp point of tip, basal circular or wealthy wedge shape, have purpura below.Flower unisexuality, dioecism; Spadix; Tool spathe, the tiny majority of male flower; In the intensive sequence of female flower, ovary 1 Room.Knot berry.Be born in remote mountains grotto mouth.The ground such as distribution Guizhou.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, this extract can significantly improve the reproductive performance of cow.
Above-mentioned purpose is achieved by following technical solution:
A kind of Chinese medicine Radix Dipsaci extract improving cow reproduction performance, prepare by the following method: (a), by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverizes, with 70 ~ 80% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 5 ~ 15% alcohol flushing, 6 ~ 10 column volumes, use 70 ~ 80% ethanol elution, 8 ~ 12 column volumes again, collect 70 ~ 80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 5% ~ 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared by the following method: (a), by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 75% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 10% alcohol flushing, 8 column volumes, use 75% ethanol elution, 10 column volumes again, collect 75% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared by the following method: (a), by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 70% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 5% alcohol flushing, 10 column volumes, use 70% ethanol elution, 12 column volumes again, collect 70% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Further, described Chinese medicine Radix Dipsaci extract is prepared by the following method: (a), by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 80% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first removes large polar component with 15% alcohol flushing, 6 column volumes, then use 80% ethanol elution, 8 column volumes, collect 80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
Above-mentioned Chinese medicine Radix Dipsaci extract is improving the application of cow reproduction aspect of performance.
Advantage of the present invention:
The Chinese medicine Radix Dipsaci extract preparation method of raising cow reproduction performance provided by the invention is simple, can suitability for industrialized production; This extract can significantly improve the reproductive performance of cow.
Detailed description of the invention
Further illustrate essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although be explained in detail the present invention with reference to preferred embodiment, those of ordinary skill in the art should be appreciated that and can modify to technical scheme of the present invention or equivalent replacement, and does not depart from essence and the scope of technical solution of the present invention.
Embodiment 1: the preparation of Radix Dipsaci extract
A (), by the Radix Dipsaci of 5kg drying and Arisaema phallospadix C. Y. Wu (accounting for gross weight 10%) mixing, pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), use petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time) successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 10% alcohol flushing, 8 column volumes, use 75% ethanol elution, 10 column volumes again, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of Radix Dipsaci extract
A (), by the Radix Dipsaci of 5kg drying and Arisaema phallospadix C. Y. Wu (accounting for gross weight 5%) mixing, pulverize, with 70% alcoholic solution circumfluence distillation (20L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), use petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time) successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 5% alcohol flushing, 10 column volumes, use 70% ethanol elution, 12 column volumes again, collect 70% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of Radix Dipsaci extract
A (), by the Radix Dipsaci of 5kg drying and Arisaema phallospadix C. Y. Wu (accounting for gross weight 15%) mixing, pulverize, with 80% alcoholic solution circumfluence distillation, merge extractive liquid, (20L × 3 time), is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 15% alcohol flushing, 6 column volumes, use 80% ethanol elution, 8 column volumes again, collect 80% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, does not add Arisaema phallospadix C. Y. Wu
A the Radix Dipsaci of 5kg drying is pulverized by (), with 75% alcoholic solution circumfluence distillation (20L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), use petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time) successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 10% alcohol flushing, 8 column volumes, use 75% ethanol elution, 10 column volumes again, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 5: the contrast of embodiment 1, Arisaema phallospadix C. Y. Wu accounts for 20% of gross weight
A (), by the Radix Dipsaci of 5kg drying and Arisaema phallospadix C. Y. Wu (accounting for gross weight 20%) mixing, pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), use petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time) successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 10% alcohol flushing, 8 column volumes, use 75% ethanol elution, 10 column volumes again, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 6: on the impact of cow reproduction performance
One, materials and methods
1, test specimen: prepare extract 1 ~ 5 according to embodiment 1 ~ 5 respectively.
2, method
2.1 experimental animals, grouping and method for breeding
Though select non-reproductive system disease, non-reproductive system deformity but cause ovary to be the Chinese XIMEN Qin 72 of resting state, non-estrus because of malnutrition, be divided into 6 groups at random, matched group 12, test group 1 ~ 5 often organizes 12.Control group fed basis forage, test group 1 ~ 5 adds the extract 1 ~ 5 of 250g/t respectively respectively on the basis of basic forage.
Test 1 month by a definite date.
2.2 statistical indicator
Respectively organize estrus of cow quantity in the statistical test time limit and oestrus by dosage, calculating each group of estrus of cow rate and oestrus and joined rate.
Two, result
As can be seen from Table 1, test group 1 ~ 3 compared with matched group, estrus of cow rate and oestrus and joined rate and be significantly increased, significant difference (P < 0.05); Test group 4 is with 5 compared with matched group, and estrus of cow rate is joined rate there was no significant difference (P > 0.05) with oestrusing.Specifically in table 1.
Table 1 extract is on estrus of cow rate and oestrus by the impact of joining rate
Result shows, extract provided by the invention can significantly improve estrus of cow rate and oestrus is joined rate.
The above-mentioned effect of extract of the present invention is relevant with the addition of whether adding Arisaema phallospadix C. Y. Wu and Arisaema phallospadix C. Y. Wu.
The effect of above-described embodiment is essentiality content of the present invention is described, but does not limit protection scope of the present invention with this.Those of ordinary skill in the art should be appreciated that and can modify to technical scheme of the present invention or equivalent replacement, and does not depart from essence and the protection domain of technical solution of the present invention.
Claims (5)
1. one kind is improved the Chinese medicine Radix Dipsaci extract of cow reproduction performance, it is characterized in that, prepare by the following method: (a) is by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 70 ~ 80% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 5 ~ 15% alcohol flushing, 6 ~ 10 column volumes, use 70 ~ 80% ethanol elution, 8 ~ 12 column volumes again, collect 70 ~ 80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 5% ~ 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
2. Chinese medicine Radix Dipsaci extract according to claim 1, is characterized in that, prepares by the following method: (a) is by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 75% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 10% alcohol flushing, 8 column volumes, use 75% ethanol elution, 10 column volumes again, collect 75% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 10% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
3. Chinese medicine Radix Dipsaci extract according to claim 1, is characterized in that, prepares by the following method: (a) is by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 70% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove large polar component with 5% alcohol flushing, 10 column volumes, use 70% ethanol elution, 12 column volumes again, collect 70% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 5% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
4. Chinese medicine Radix Dipsaci extract according to claim 1, is characterized in that, prepares by the following method: (a) is by the Radix Dipsaci of drying and Arisaema phallospadix C. Y. Wu mixing, pulverize, with 80% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first removes large polar component with 15% alcohol flushing, 6 column volumes, then use 80% ethanol elution, 8 column volumes, collect 80% ethanol elution, concentrating under reduced pressure; Wherein, Arisaema phallospadix C. Y. Wu accounts for 15% of Radix Dipsaci and Arisaema phallospadix C. Y. Wu gross weight.
5. the arbitrary described Chinese medicine Radix Dipsaci extract of Claims 1 to 4 is improving the application of cow reproduction aspect of performance.
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Application publication date: 20160511 |