CN105902664A - Traditional Chinese medicine Chinese soapberry fruit extract for treating diarrhea of yak calves - Google Patents
Traditional Chinese medicine Chinese soapberry fruit extract for treating diarrhea of yak calves Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The invention discloses a traditional Chinese medicine Chinese soapberry fruit extract for treating diarrhea of yak calves. A method for preparing the Chinese soapberry fruit extract comprises the following steps: (a) mixing dried Chinese soapberry fruits with marigolds, crushing the mixture, carrying out heat reflux extraction by using a 85-95% alcohol solution, merging the extracting solution, and carrying out concentrating until the mixed solution is free from alcohol taste to obtain an alcohol extraction concentrated solution; (b) diluting the alcohol extraction concentrated solution obtained in the step (a) with water, sequentially extracting the obtained diluent by using petroleum ether, ethyl acetate and water-saturated n-butyl alcohol, and carrying out vacuum concentration to respectively obtain petroleum ether extract, ethyl acetate extract and n-butyl alcohol extract; and (c) dissolving the n-butyl alcohol extract with water, carrying out filtering, enriching active ingredients by using AB-8 type macroporous resin, firstly using 10-20% alcohol to wash 8-12 column volume so as to remove high-polarity ingredients, then using 85-95% alcohol to elute 14-16 column volume, collecting the eluant of the 85-95% alcohol, and carrying out vacuum concentration, wherein the weight of the marigolds accounts for 5-15% of the total weight of the Chinese soapberry fruits and marigolds.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Chinese medicine soapberry extract treating Yak calf diarrhoea, should
Extract has obvious therapeutical effect to Yak calf diarrhoea.
Background technology
Yak calf diarrhoea is the disease that pastoral area is common, and M & M is higher, the development of serious threat animal husbandry and production effect
Benefit.The cause of disease complexity causing diarrhoea is various, and the factor such as antibacterial, virus, parasite, dyspepsia and environment all can cause diarrhoea
Generation, wherein enteropathogenic Escherichia coli and salmonella are the main pathogen causing Yak calf to suffer from diarrhoea.
Fructus Sapindi Mukouossi is that sapindaceous plant is without suffering from the seed of tree.Harvesting ripe fruit, removes sarcocarp, takes seed and dry.Former plant
It is fallen leaves or aiphyllium without suffering from tree, up to 25 meters.Branch is carried out, sprig without hair, close raw most hole skins；Hibernaculum axil is raw, has outward
Scale 2 is right, slightly fine, soft fur. usually even numbers winglike compound leaf, alternate；Without stipule；There is handle；Lobule 8～12 pieces, wide lanceolar
Or oval, long 6～15 centimetres, wide 2.5～5 centimetres, tip length point, Quan Yuan, the wealthy wedge shape of base portion or tiltedly circular, left and right is not
Deng, keratin, without hair, or there is microtriche on following master pulse；Petiolule is extremely short.Panicle, top is raw and side is raw；Flower polygamy is little
Shape, stockless, line shaft and branch are all by khaki fine, soft fur；Calyx 5, outer 2 short, interior 3 longer, circular or oval；
Corolla light green, 5 lobes, avette to ovum shape lanceolar, there is short pawl；Floral disc cup-shaped；Male flower has 8～10 pieces of flourishing stamens,
And be born in inside floral disc, it is raw that filigree has fine, soft fur, medicine back；Female flower, ovary is upper, and Room the most only 1 is grown；Hermaphrodite flower is male
Stamen is little, and filigree has fur.Drupe is spherical, about 15～20 millimeters of footpath, yellow or brown color time ripe.Seed is spherical, black, footpath
About 12～15 millimeters.Florescence 6～July.Really phase 9～October.Distribution Anhui, Jiangsu, Zhejiang, Jiangxi, Hubei, Hunan,
The ground such as Fujian, Taiwan, Guangdong, Guangxi, Sichuan, Guizhou, Shaanxi.Produce the ground such as Guangdong, Guangxi.
Flos Tagetis Erectae is the inflorescence of feverfew Flos Tagetis Erectae.Pluck between summer, autumn.Former plant Flos Tagetis Erectae is annual herb；Sturdy directly
Vertical, high about 60 casket rice, what entirety was rubbed has corrupt abnormal smells from the patient.Leaf to life, pinniform drastic crack, sliver square is circular or lanceolar, long 1.2～
2.5 centimetres, edge has sawtooth, proximal edge to have several pieces of big bodies of gland, and the tip of some sliver or increment have a long awns.Head inflorescence list
Raw, Yellow-to-orange, diameter 5～10 centimetres；Common peduncle is sturdy；Involucre mitriform, tooth extends；Ligulate flower is most, has long handle,
The outer outside warp of row tongue piece.Achene is linear, titled with 1～2 piece of long aristiform and 2～3 pieces of short and blunt scales.Autumn at florescence, winter.
There is cultivation China various places.
Have not yet to see the application in terms for the treatment of Yak calf diarrhoea of Fructus Sapindi Mukouossi and Flos Tagetis Erectae.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine soapberry extract treating Yak calf diarrhoea, Yak calf is suffered from diarrhoea by this extract
There is obvious therapeutical effect.
Above-mentioned purpose is achieved by following technical solution:
A kind of Chinese medicine soapberry extract treating Yak calf diarrhoea, is prepared via a method which: (a) by dry Fructus Sapindi Mukouossi and
Flos Tagetis Erectae mixes, and pulverizes, and with 85～95% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol without alcohol taste and carry
Take concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water successively
Saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；
C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 10～20% ethanol
Rinse 8～12 column volumes and remove big polar components, then with 85～95% ethanol elution 14～16 column volumes, collection 85～95%
Ethanol elution, concentrating under reduced pressure；Wherein, Flos Tagetis Erectae accounts for the 5%～15% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Further, described Chinese medicine soapberry extract is prepared via a method which: (a) is by dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae
Mixing, pulverizes, with 90% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 10 column volumes of 15% alcohol flushing
Remove big polar component, then with 15 column volumes of 90% ethanol elution, collect 90% ethanol elution, concentrating under reduced pressure；Wherein,
Flos Tagetis Erectae accounts for the 10% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Further, described Chinese medicine soapberry extract is prepared via a method which: (a) is by dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae
Mixing, pulverizes, with 85% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 12 column volumes of 10% alcohol flushing
Remove big polar component, then with 16 column volumes of 85% ethanol elution, collect 85% ethanol elution, concentrating under reduced pressure；Wherein,
Flos Tagetis Erectae accounts for the 5% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Further, described Chinese medicine soapberry extract is prepared via a method which: (a) is by dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae
Mixing, pulverizes, with 95% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses petroleum ether, ethyl acetate and water saturated positive fourth successively
Alcohol extracts, and concentrating under reduced pressure respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol
Extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first with 8 column volumes of 20% alcohol flushing
Remove big polar component, then with 14 column volumes of 95% ethanol elution, collect 95% ethanol elution, concentrating under reduced pressure；Wherein,
Flos Tagetis Erectae accounts for the 15% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
The application in terms for the treatment of Yak calf diarrhoea of the above-mentioned Chinese medicine soapberry extract.
Advantages of the present invention:
The Chinese medicine soapberry extract preparation method of the treatment Yak calf diarrhoea that the present invention provides is simple, can industrialized production；This carries
Take thing and can effectively treat Yak calf diarrhoea.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of soapberry extract
A Fructus Sapindi Mukouossi and Flos Tagetis Erectae (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 90% ethanol solution hot reflux
Extract (20L × 3 time), united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) institute
Ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and
Water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and
N-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first
Remove big polar component with 10 column volumes of 15% alcohol flushing, then with 15 column volumes of 90% ethanol elution, collect 90% second
Alcohol eluen, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of soapberry extract
A Fructus Sapindi Mukouossi and Flos Tagetis Erectae (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 85% ethanol solution hot reflux
Extract (20L × 3 time), united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) institute
Ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and
Water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and
N-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first
Remove big polar component with 12 column volumes of 10% alcohol flushing, then with 16 column volumes of 85% ethanol elution, collect 85% second
Alcohol eluen, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of soapberry extract
A Fructus Sapindi Mukouossi and Flos Tagetis Erectae (accounting for gross weight 15%) that 5kg is dried by () mix, and pulverize, with 95% ethanol solution hot reflux
Extract, united extraction liquid (20L × 3 time), be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) institute
Ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and
Water saturated n-butyl alcohol (3L × 3 time) extract, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and
N-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first
Remove big polar component with 8 column volumes of 20% alcohol flushing, then with 14 column volumes of 95% ethanol elution, collect 95% second
Alcohol eluen, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, without Flos Tagetis Erectae
A Fructus Sapindi Mukouossi that 5kg is dried by () is pulverized, with 90% ethanol solution circumfluence distillation (20L × 3 time), united extraction
Liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；(b) by step (a) gained ethanol extraction concentrated solution dilute with water (3L),
Successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), subtract
Pressure concentrates, and respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；(c) n-butyl alcohol extract water
Dissolve, filter, with AB-8 type macroporous resin enrichment active component, first remove big polarity with 10 column volumes of 15% alcohol flushing
Composition, then with 15 column volumes of 90% ethanol elution, collects 90% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 5: the contrast of embodiment 1, Flos Tagetis Erectae accounts for the 20% of gross weight
A Fructus Sapindi Mukouossi and Flos Tagetis Erectae (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 90% ethanol solution hot reflux
Extract (20L × 3 time), united extraction liquid, be concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) institute
Ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and
Water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and
N-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first
Remove big polar component with 10 column volumes of 15% alcohol flushing, then with 15 column volumes of 90% ethanol elution, collect 90% second
Alcohol eluen, concentrating under reduced pressure and get final product.
Embodiment 6: the therapeutical effect to Yak calf diarrhoea
One, materials and methods
1, material: embodiment 1～5 is shown in the preparation of extract.
2, method
2.1 experimental animals and packet
Experimental animal is 60 diarrhoea wheat low-lying areas Yak calf (being provided by Sichuan Province's stud farm) at 2 randomly selected～3 monthly ages.Its
In, as a control group, 50 diarrhoea wheat low-lying area Yak calf are randomly divided into test group 1～5 to 10 diarrhoea wheat low-lying area Yak calf, often organize 10
Only.Matched group uses Basic drawing to feed.Test group 1～5 adds the reality of 800mg/kg respectively on the basis of Basic drawing respectively
Execute the extract 1～5 of example 1～5 preparation.
2.2 method for breeding
5 days test periods.Use routine to raise and immunity during whole test, free choice feeding, freely drink water.
2.3 efficacy determination indexs
Cure rate: during test, after being administered, yak symptom of diarrhea disappears, defecation frequency, feces character, spirit, sign
Recover normal, it is determined that for curing.Cure number and account for the percentage ratio i.e. cure rate of test sum.
Effective percentage: during test, after being administered, yak diarrhoea alleviates or disappears, defecation frequency, feces character, spirit, body
Levy and taken a turn for the better, it is determined that for effectively.Significant figure accounts for the percentage ratio i.e. effective percentage of test sum.
Inefficiency: during test, after being administered, yak symptom of diarrhea does not alleviates or increases the weight of or dead, it is determined that for invalid.Invalid
Number accounts for the percentage ratio i.e. inefficiency of test sum.
Two, result
The results are shown in Table 1.
As shown in Table 1, the therapeutical effect of test group 1～3 pairs of Yak calf diarrhoea is significantly higher than matched group, and difference has significance (P
＜ 0.05)；The therapeutical effect of test group 4 and 5 pairs of Yak calf diarrhoea there was no significant difference (P ＞ 0.05) with matched group.
The therapeutical effect that Yak calf is suffered from diarrhoea by table 1 extract
| Group | Quantity | Inefficiency (invalid number) | Cure rate (healing number) | Effective percentage (significant figure) |
| Matched group | 10 | 90% (9) | - | 10% (1) |
| Test group 1 | 10 | 0(0) | 90% (9) | 100% (10) |
| Test group 2 | 10 | 0(0) | 80% (8) | 100% (10) |
| Test group 3 | 10 | 0(0) | 80% (8) | 100% (10) |
| Test group 4 | 10 | 80% (8) | 0(0) | 20% (2) |
| Test group 5 | 10 | 80% (8) | 0(0) | 20% (2) |
Result shows, the extract that the present invention provides has significant therapeutic effect to Yak calf diarrhoea.
The above-mentioned effect of extract of the present invention is relevant with the addition whether adding Flos Tagetis Erectae and Flos Tagetis Erectae.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (5)
1. the Chinese medicine soapberry extract treating Yak calf diarrhoea, it is characterised in that be prepared via a method which: (a)
Dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae are mixed, pulverizes, with 85～95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into
Taste without alcohol obtains ethanol extraction concentrated solution；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, uses oil successively
Ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract
And n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component,
First with 10～20% alcohol flushing 8～12 column volumes remove big polar components, then with 85～95% ethanol elution 14～16 cylinders
Long-pending, collection 85～95% ethanol elution, concentrating under reduced pressure；Wherein, Flos Tagetis Erectae accounts for the 5%～15% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Chinese medicine soapberry extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae are mixed, pulverizes, with 90% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into nothing
Alcohol taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
15% alcohol flushing 10 column volume removes big polar component, then with 15 column volumes of 90% ethanol elution, collects 90% ethanol
Eluent, concentrating under reduced pressure；Wherein, Flos Tagetis Erectae accounts for the 10% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Chinese medicine soapberry extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae are mixed, pulverizes, with 85% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into nothing
Alcohol taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
10% alcohol flushing 12 column volume removes big polar component, then with 16 column volumes of 85% ethanol elution, collects 85% ethanol
Eluent, concentrating under reduced pressure；Wherein, Flos Tagetis Erectae accounts for the 5% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
Chinese medicine soapberry extract the most according to claim 1, it is characterised in that be prepared via a method which: (a)
Dry Fructus Sapindi Mukouossi and Flos Tagetis Erectae are mixed, pulverizes, with 95% ethanol solution circumfluence distillation, united extraction liquid, be concentrated into nothing
Alcohol taste obtains ethanol extraction concentrated solution；(b) by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether,
Ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtain petroleum ether extract, acetic acid ethyl ester extract and just
Butanol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first uses
20% alcohol flushing 8 column volume removes big polar component, then with 14 column volumes of 95% ethanol elution, collects 95% ethanol and wash
De-liquid, concentrating under reduced pressure；Wherein, Flos Tagetis Erectae accounts for the 15% of Fructus Sapindi Mukouossi and Flos Tagetis Erectae gross weight.
5. Claims 1 to 4 arbitrary described Chinese medicine soapberry extract application in terms for the treatment of Yak calf diarrhoea.
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| CN102228656A (en) * | 2011-06-20 | 2011-11-02 | 施瑞客（天津）生物技术有限公司 | Chinese medicinal composition for treating bovine diarrhoea |
| CN105147907A (en) * | 2015-10-22 | 2015-12-16 | 淄博夸克医药技术有限公司 | Dasheen flower extract, as well as preparation method and medicinal application thereof |
| CN105534814A (en) * | 2016-01-19 | 2016-05-04 | 郭根松 | Plant-extract-based freckle removing and whitening cosmetic |
| CN105560669A (en) * | 2016-03-22 | 2016-05-11 | 吴正锋 | Traditional Chinese medicine teasel root extract for improving reproductive performance of cows |
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