CN105707489A - Traditional Chinese medicine scrophularia ningpoensis extract for improving productivity and muscle quality of pork pigs - Google Patents
Traditional Chinese medicine scrophularia ningpoensis extract for improving productivity and muscle quality of pork pigs Download PDFInfo
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- CN105707489A CN105707489A CN201610163907.9A CN201610163907A CN105707489A CN 105707489 A CN105707489 A CN 105707489A CN 201610163907 A CN201610163907 A CN 201610163907A CN 105707489 A CN105707489 A CN 105707489A
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Abstract
The invention discloses traditional Chinese medicine scrophularia ningpoensis extract for improving productivity and muscle quality of pork pigs. The method for preparing the scrophularia ningpoensis extract comprises the steps that a, dry scrophularia ningpoensis and biond magnolia flowers are mixed, smashed and extracted with a 70-80% ethanol solution in a heat reflux mode, and extracting solutions are combined and concentrated to be free of alcohol flavor to obtain an ethanol extraction concentrated solution; b, the ethanol extraction concentrated solution obtained in the step a is diluted with water, extracted with petroleum ether, ethyl acetate and water saturated n-butyl alcohol in sequence, and concentrated in a pressure reduction mode to obtain petroleum ether extract, ethyl acetate extract and n-butyl extract; c, n-butyl extract is dissolved with water and filtered, D101-type macroporous resin is used for enriching active ingredients, 5-15% ethyl alcohol is used for flushing 6-10 column volume to remove large polarity ingredients, then 70-80% ethyl alcohol is used for eluting 8-12 column volume, 70-80% ethanol eluant is collected, and pressure-reduction concentrating is carried out; the biond magnolia flowers account for 5-15% of the total weight of scrophularia ningpoensis and the biond magnolia flowers.
Description
Technical field
The invention belongs to animal husbandry, relate to veterinary drug, be specifically related to a kind of Studying Chinese Crude Drug Figwort root extract improving barren sow production performance and meat quality, this extract can significantly improve barren sow and produce performance and meat quality.
Background technology
Studying Chinese Crude Drug Figwort root is the root of goatweed Radix Scrophulariae.Excavate in the Beginning of Winter of around, remove stem, leaf, fibrous root, outwash silt, tan by the sun 5～6 days, and often stir, Caulis et Folium Oryzae antifreeze (enduring cold then hollow) must be added a cover every night, shine to time half-dried, pile up 2～3 days, make internal blackening then row Exposure to Sunlight, and repeatedly piling, shining, until being completely dried.Overcast and rainy take oven drying method.The easy counter tide of this product, should store in aeration-drying place, it is prevented that mildews and damages by worms.Former plant Radix Scrophulariae is perennial herb, high 60～120 centimetres.Root is cylindrical, long 5～12 centimetres, diameter 1.5～3 centimetres, the normal bifurcated in bottom, crust ash yellowish-brown.Stem is upright, four shapes, smooth or have gland shape pubescence.Leaf is to life；Petiole length 0.5～2 centimetre；Blade is avette or ovum shape oval, and long 7～20 centimetres, wide 3.5～12 centimetres, tip is tapering, basal circular or closely cut shape, and edge tool crenation has sparse scattered fine, soft fur below.Cyme is evacuated and is carried out, in coniform；Hua Gengshi 1～3 casket rice, inflorescence and bennet have obvious glandular hair；Sepal 5 splits, oval, and tip is blunt, and there is gland shape fine, soft fur outside；Corolla mulberry, the oblique gyalectiform in pipe portion, be about 8 millimeters, have 5 slivers, above 2 split longer and big, side 2 sliver takes second place, below sliver minimum；Stamen 4, the last 2, separately there is 1 piece of stamen degenerated, in flakey, adhesion is on corolla pipe；Floral disc is obvious；Ovary is upper, and Room 2, style is elongated.Capsule oval, the short point of tip, dark green or dirty-green, it is about 8 millimeters, calyx harbors.7～August of florescence.Fruit phase 8～JIUYUE.It is grown in hillside sylvan life.The ground such as distribution Anhui, Jiangsu, Zhejiang, Fujian, Jiangxi, Hunan, Hubei, Guizhou, Shaanxi.There is a large amount of cultivation in Zhejiang, and also there is cultivation other various places.Main product Zhejiang, Sichuan, Hubei.Additionally, the ground such as Guizhou, Hunan, Jiangxi also produce.Big with Zhejiang yield, quality is good.
Chinese medicinal magnolia flower is the alabastrum of Magnoliacea plant Flos Magnoliae.It is typically in early spring alabastrum to pluck when not putting, cuts discarding stalk, dry.Former plant Flos Magnoliae is machaka, high 3～4 meters.Dried bark canescence；Sprig puce, smooth without hair, the vertical oblong hole skin of tool, shallow brown in vain；Pushing up raw hibernaculum avette, long 1～1.5 centimetre, by light gray green thin,tough silk hair, axillalry bud is little, long 2～3 millimeters.Leaf alternate, has short handle, and handle is long 1.5-2 centimetre, without hair, sometimes slightly has undercoat；Blade is oval or the shape of falling ovum is oval, and long 10～16 centimetres, wide 5～8.5 centimetres, tip is tapering, basal circular, or in circle wedge shape, Quan Yuan, the equal Glabrous in two sides, extremely rare undercoat is sometimes had in leaf margin place, surface is green, back side light green color, and master pulse protrudes.Open before Hua Yuye, or closely open simultaneously, single, it is born in sprig top；3, calyx, green, ovum shape lanceolar, it is about 1/4～1/3 into petal, generally early de-；6, corolla, outside aubergine, inner face white, obovate, long 8 cm, stamen is most, and helical arrangement, flower pesticide is linear, and filigree is short；Carpel majority separates, and also helical arrangement, style is short and small tapering.Fruit oblong, sometimes slightly bent.2～May of florescence.It is grown on warmer area.Former distribution Hubei, Anhui, Zhejiang, Fujian one are with, present wild less, and in Shandong, Sichuan, Jiangxi, Hubei, Yunnan, Southern Shaanxi, the ground such as Henan extensively cultivates.
Summary of the invention
It is an object of the invention to provide a kind of Studying Chinese Crude Drug Figwort root extract improving barren sow production performance and meat quality, this extract can significantly improve barren sow and produce performance and meat quality.
Above-mentioned purpose is achieved by following technical solution:
A kind of Studying Chinese Crude Drug Figwort root extract improving barren sow production performance and meat quality, it is prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 70～80% alcoholic solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6～10 column volumes of 5～15% alcohol flushing, again with 8～12 column volumes of 70～80% ethanol elution, collect 70～80% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 5%～15% of Radix Scrophulariae and Flos Magnoliae gross weight.
Further, described Studying Chinese Crude Drug Figwort root extract is prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 75% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 10% of Radix Scrophulariae and Flos Magnoliae gross weight.
Further, described Studying Chinese Crude Drug Figwort root extract is prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 70% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 5% of Radix Scrophulariae and Flos Magnoliae gross weight.
Further, described Studying Chinese Crude Drug Figwort root extract is prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 15% of Radix Scrophulariae and Flos Magnoliae gross weight.
The application in improving barren sow and producing performance and meat quality of the above-mentioned Studying Chinese Crude Drug Figwort root extract.
Advantages of the present invention:
The Studying Chinese Crude Drug Figwort root method for preparing extractive that raising barren sow provided by the invention produces performance and meat quality is simple, can industrialized production；This extract can significantly improve barren sow and produce performance and meat quality, pure natural, safely and effectively.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of Radix Scrophulariae extract
A Radix Scrophulariae and Flos Magnoliae (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, again with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 2: the preparation of Radix Scrophulariae extract
A Radix Scrophulariae and Flos Magnoliae (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 70% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, again with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 3: the preparation of Radix Scrophulariae extract
A Radix Scrophulariae and Flos Magnoliae (accounting for gross weight 15%) that 5kg is dried by () mix, and pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid (20L × 3 time), are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), extract with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) successively, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, again with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure and get final product.
Embodiment 4: the contrast of embodiment 1, without Flos Magnoliae
A Radix Scrophulariae mixing that 5kg is dried by (), pulverizes, with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure.
Embodiment 5: the contrast of embodiment 1, Flos Magnoliae accounts for the 20% of gross weight
A Radix Scrophulariae and Flos Magnoliae (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 75% alcoholic solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure.
Embodiment 6: the impact on Performance of Finishing Pigs and meat quality
One, materials and methods
1, material: embodiment 1～5 is shown in the preparation of extract, prepares Radix Scrophulariae extract 1～5 respectively.
2, method
2.1 experimental animals and packet
Consistent according to parity, nutriture, body weight are close, castrate the principle of male and female half and half, select DLY three way cross nursery pig 60, are randomly divided into 6 groups, often group 10, carries out feeding until delivering for sale.Matched group adopts general material of fattening, and test group 1～5 adds the Radix Scrophulariae extract 1～5 of 250g/t respectively respectively on the basis fatten material.
2.2 method for breeding
Test first carries out 1 week preliminary trial period, and preliminary trial period unifies anthelmintic, after prerun 1 week, and beginning formal test, test pig feeds in units of hurdle, until delivering for sale, free choice feeding and drinking-water.Record feed consumption rate, health status etc..
2.3 test indexs and method
According to body weight after having tested, often group randomly chooses 22 mothers, butchers.Measure the Antioxidant Indexes in meat and muscle.
Growth performance: statistics initial weight, end weight, Average weight increasing a day (ADG) and feed-weight ratio.
Meat Quality: pH, it is waterpower, yellowish pink, drip loss, marble grain scoring etc., is measured according to the main meat quality determination techniques specification of NY-T821-2004.Take psoas major and measure tenderness.
Antioxidant Indexes in muscle: take eye muscle sample in butchering rear 12h-15 DEG C of quick-freezings, go breast section meat sample 100g homogenate after defrosting, glutathion peroxidase (GSH-PX), superoxide dismutase (SOD), malonaldehyde (MDA) mensuration all adopt Nanjing to build up Bioengineering Research Institute's biochemical reagents box description to carry out.
3, statistical analysis
Test data adopts SPSS20.0 software to carry out variance analysis, and the significance of difference carries out Duncans multiple comparisons, and in table, numeric representation is Mean ± SD.
Two, result
1, the extract impact on Performance of Finishing Pigs in diet
As shown in Table 1, the end weight of test group 1～3 and Average weight increasing a day are above matched group, and feed-weight ratio is below matched group, significant difference (P < 0.05) compared with matched group；The end weight of test group 4 and 5 and Average weight increasing a day there was no significant difference (P > 0.05) with matched group, and feed-weight ratio and matched group there was no significant difference (P > 0.05).
The impact on Performance of Finishing Pigs of table 1 extract
2, the extract impact on meat quality in diet
As shown in Table 2, test group 1～3 with matched group compare yellowish pink, be waterpower, drip loss in be respectively provided with significant difference (P < 0.05), difference inconspicuous (P > 0.05) in tenderness and marble grain scoring；Test group 4 with 5 with matched group compare yellowish pink, be waterpower, drip loss, tenderness and marble grain scoring in equal difference inconspicuous (P > 0.05).
The impact on meat quality of table 2 extract
3, in diet extract on the impact of antioxidation in growing and fattening pigs muscle
As shown in Table 3, test group 1～3 is compared Antioxidant Indexes with matched group and is all significantly improved (P < 0.05)；Test group 4 with 5 with matched group difference (P > 0.05) of comparing that there are no significant.
Table 3 extract is on the impact of antioxidation in growing and fattening pigs muscle
It is shown that Radix Scrophulariae extract provided by the invention improves antioxidation in Performance of Finishing Pigs, meat quality and growing and fattening pigs muscle.The decline of meat quality, mostly due to after butchering, a large amount of lipid peroxide generation oxidation reactions in muscle, destroy cell membrane, thus cause pH to decline, yellowish pink thin out and be degradation under waterpower.And extract provided by the invention can enter internal scavenging free radicals, reducing peroxide generation oxidation reaction, protecting muscle cell integrity, thus improving meat quality.
The above-mentioned effect of extract of the present invention is relevant with the addition whether adding Flos Magnoliae and Flos Magnoliae.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.It will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from essence and the protection domain of technical solution of the present invention.
Claims (5)
1. the Studying Chinese Crude Drug Figwort root extract improving barren sow production performance and meat quality, it is characterized in that, it is prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 70～80% alcoholic solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6～10 column volumes of 5～15% alcohol flushing, again with 8～12 column volumes of 70～80% ethanol elution, collect 70～80% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 5%～15% of Radix Scrophulariae and Flos Magnoliae gross weight.
2. Studying Chinese Crude Drug Figwort root extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 75% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 10% alcohol flushing, then with 10 column volumes of 75% ethanol elution, collect 75% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 10% of Radix Scrophulariae and Flos Magnoliae gross weight.
3. Studying Chinese Crude Drug Figwort root extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 70% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 5% alcohol flushing, then with 12 column volumes of 70% ethanol elution, collect 70% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 5% of Radix Scrophulariae and Flos Magnoliae gross weight.
4. Studying Chinese Crude Drug Figwort root extract according to claim 1, it is characterised in that be prepared via a method which: dry Radix Scrophulariae and Flos Magnoliae are mixed by (a), pulverize, with 80% alcoholic solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with D101 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 15% alcohol flushing, then with 8 column volumes of 80% ethanol elution, collect 80% ethanol elution, concentrating under reduced pressure；Wherein, Flos Magnoliae accounts for the 15% of Radix Scrophulariae and Flos Magnoliae gross weight.
5. Claims 1 to 4 arbitrary described Studying Chinese Crude Drug Figwort root extract application in improving barren sow and producing performance and meat quality.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115487242A (en) * | 2022-11-17 | 2022-12-20 | 云南英格生物技术有限公司 | Radix scrophulariae extract and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115487242A (en) * | 2022-11-17 | 2022-12-20 | 云南英格生物技术有限公司 | Radix scrophulariae extract and preparation method and application thereof |
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Application publication date: 20160629 |